pycom520g00640

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0010272
Physical Location & Seq
Forward (+)
554172 .. 559205
5034 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00640.8

Sequence Viewer

Length: 1380 bp
ATGGTGATGATCCGCAGTCTCAGTCGCAGTCTCGGTCGCAGTGCCATCGCCAAAACACTCCTCTCGCCATCAAATTCAAGGCCCTCTTCTTCTTCATCCTCTGCCCATTCTGCTATTCCGTTGTTGGCGCCGTTAGCGGGTCAACGCCTACGCAACAACTCTGCGGCATTTGAACCCGAATTCAGAGATACCACCACCAGCACCACCAGATGCCTTCACTCCAACGCCTCGCTTGGCGGCGGTGGCGGACTGCCTTCCTCCATGAGGGCAGCTGTCTTCAGGGAACCCAACAAACCTCTCACCTTCGAGGAATTTCACATCCCTCGCCCCAAATCCGGTGAACTTCTCATCAAAACCAAGGCATGCGGAGTTTGCCACTCTGATCTCCATGTTATGAAAGGCGAGCTTCCATTTGCTAGCCCATGTGTTGTGGGACATGAGATAACTGGTGAGGTTGTCGAACATGGACCGGCCACAGACACCAAAACTATAGCAAGATTACCAATTGGATCTCGAGTTGTTGGAGCTTTCATCATGCCTTGTGGCAACTGTTCCTACTGTTCAAAGGGCCACGATGATCTATGCGAGGATTTCTTTGCTTATAACCGTGCAAAAGGAACTCTTTATGATGGTGAAACTCGGCTGTTCCTCCGCAGCAGTGGAGAACCAGTATACATGTATAGTATGGGAGGCCTTGCTGAATACTGTGTTGTGCCGGCAAATGCATTGACTATTTTGCCCGAGTCATTGCCATACATCGAGTCTGCAGTTTTAGGTTGTGCAGTGTTTACCGCTTATGGTACTATGGCTAATGCGGCTCAGGTGAAGCCTGGGGATTCAGTTGCTGTGATTGGAATTGGGGGTGTAGGGTCAAGCTGTTTGCAGATAGCAAGAGCATTTGGTGCCTCTGATATTATCGCTGTGGATGTTCAAGATGAAAAACTGCAGAAAGCCAAGATATTTGGAGCTACGCATGCTGTAAATGCAGCTAAGGAAGATGCTGTTGGCAAAATAATGGAAATAACTGGTGGAAAGGGTGTGGACATCGCTGTGGAAGCCCTGGGAAGGCCGCAGACATTTTTGCAATGTGTGCAAAGTGTGAAAGATGGAGGAAAAGCTGTAATGGTTGGGCTTTCACAAGCTGGTGCAGTTGGGGAAGTAGATATAAACCGTCTTGTCCGTAGAAAGATACAAATCATTGGATCTTACGGAGGGAGGGCAAGGCAGGATCTGCCAAAGTTGGTCAGATTGGCAGAATCAGGCGTATTTGATCTAGCTAATGCTGTTTCTAGAAGGTACAAATTTGAGGAGAGTAACGAAGCATTCCAGGAACTCAATAAGGGAAGCATTGTTGGTCGGGCAGTGGTTGAGATAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

460

Amino Acids

48.56

Weight (kDa)

8.53

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccB1I GGYRCC 2 cut(s) 127, 902
AccI GTMKAC 1 cut(s) 672
AclWI GGATC 4 cut(s) 4, 517, 1210, 1236
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 4 cut(s) 73, 179, 311, 1301
AcuI CTGAAG 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 2 cut(s) 802, 1298
AfiI CCNNNNNNNGG 4 cut(s) 137, 264, 335, 1065
AflIII ACRYGT 1 cut(s) 675
AgsI TTSAA 4 cut(s) 78, 173, 564, 932
AjnI CCWGG 3 cut(s) 829, 1059, 1326
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
AluBI AGCT 9 cut(s) 272, 406, 527, 876, 968, 989, 1118, 1142, 1277
AluI AGCT 9 cut(s) 272, 406, 527, 876, 968, 989, 1118, 1142, 1277
Alw26I GTCTC 2 cut(s) 23, 35
AlwI GGATC 4 cut(s) 4, 517, 1210, 1236
AlwNI CAGNNNCTG 2 cut(s) 845, 1231
Ama87I CYCGRG 2 cut(s) 513, 740
AoxI GGCC 5 cut(s) 80, 471, 568, 691, 1067
ApeKI GCWGC 3 cut(s) 269, 654, 986
ApoI RAATTY 4 cut(s) 73, 179, 311, 1301
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 3 cut(s) 81, 467, 568
AsuHPI GGTGA 6 cut(s) 16, 292, 350, 461, 644, 835
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 2 cut(s) 513, 740
AvaII GGWCC 1 cut(s) 467
BanI GGYRCC 2 cut(s) 127, 902
BbsI GAAGAC 1 cut(s) 268
BbvI GCAGC 3 cut(s) 281, 666, 998
BccI CCATC 4 cut(s) 53, 76, 623, 1100
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 3 cut(s) 831, 1061, 1328
BcoDI GTCTC 2 cut(s) 23, 35
BfaI CTAG 3 cut(s) 417, 1274, 1290
BfmI CTRYAG 3 cut(s) 489, 765, 944
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 7 cut(s) 165, 238, 270, 655, 816, 987, 1070
BlsI GCNGC 7 cut(s) 166, 239, 271, 656, 817, 988, 1071
Bme1390I CCNGG 3 cut(s) 831, 1061, 1328
Bme18I GGWCC 1 cut(s) 467
BmeT110I CYCGRG 2 cut(s) 513, 740
BmgT120I GGNCC 3 cut(s) 81, 467, 568
BmiI GGNNCC 3 cut(s) 129, 285, 904
BmrFI CCNGG 3 cut(s) 831, 1061, 1328
BmsI GCATC 2 cut(s) 200, 988
BmtI GCTAGC 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 268
Bpu10I CCTNAGC 2 cut(s) 819, 990
BsaBI GATNNNNATC 1 cut(s) 1193
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 357, 830, 1059, 1060
BsaWI WCCGGW 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 264, 335, 1065
Bse118I RCCGGY 2 cut(s) 469, 715
Bse1I ACTGG 3 cut(s) 451, 668, 1030
Bse3DI GCAATG 2 cut(s) 746, 1091
Bse8I GATNNNNATC 1 cut(s) 1193
BseBI CCWGG 3 cut(s) 831, 1061, 1328
BseDI CCNNGG 4 cut(s) 357, 830, 1059, 1060
BseGI GGATG 3 cut(s) 95, 318, 931
BseJI GATNNNNATC 1 cut(s) 1193
BseLI CCNNNNNNNGG 4 cut(s) 137, 264, 335, 1065
BseMI GCAATG 2 cut(s) 746, 1091
BseMII CTCAG 2 cut(s) 34, 833
BseNI ACTGG 3 cut(s) 451, 668, 1030
BseRI GAGGAG 2 cut(s) 50, 1322
BseXI GCAGC 3 cut(s) 281, 666, 998
BsgI GTGCAG 2 cut(s) 801, 1167
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 5 cut(s) 82, 473, 570, 693, 1069
BshNI GGYRCC 2 cut(s) 127, 902
BsiEI CGRYCG 1 cut(s) 37
BsiHKCI CYCGRG 2 cut(s) 513, 740
BsiSI CCGG 3 cut(s) 336, 470, 716
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 4 cut(s) 137, 264, 335, 1065
BsmAI GTCTC 2 cut(s) 23, 35
BsmFI GGGAC 1 cut(s) 447
BsmI GAATGC 1 cut(s) 1322
BsnI GGCC 5 cut(s) 82, 473, 570, 693, 1069
BsoBI CYCGRG 2 cut(s) 513, 740
Bsp143I GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
BspANI GGCC 5 cut(s) 82, 473, 570, 693, 1069
BspCNI CTCAG 2 cut(s) 33, 832
BspLI GGNNCC 3 cut(s) 129, 285, 904
BspMAI CTGCAG 2 cut(s) 769, 948
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 4 cut(s) 4, 517, 1210, 1236
BspT107I GGYRCC 2 cut(s) 127, 902
BsrDI GCAATG 2 cut(s) 746, 1091
BsrFI RCCGGY 2 cut(s) 469, 715
BsrI ACTGG 3 cut(s) 451, 668, 1030
BssAI RCCGGY 2 cut(s) 469, 715
BssECI CCNNGG 4 cut(s) 357, 830, 1059, 1060
BssMI GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
BssNAI GTATAC 1 cut(s) 673
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 357
Bst1107I GTATAC 1 cut(s) 673
Bst2UI CCWGG 3 cut(s) 831, 1061, 1328
Bst4CI ACNGT 5 cut(s) 551, 560, 608, 707, 1172
Bst6I CTCTTC 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 128
BstAPI GCANNNNNTGC 3 cut(s) 902, 1090, 1231
BstC8I GCNNGC 5 cut(s) 364, 404, 418, 717, 975
BstDEI CTNAG 3 cut(s) 20, 819, 990
BstENI CCTNNNNNAGG 1 cut(s) 262
BstF5I GGATG 3 cut(s) 95, 318, 931
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 7 cut(s) 12, 385, 512, 580, 1205, 1231, 1273
BstMAI GTCTC 2 cut(s) 23, 35
BstMBI GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
BstMCI CGRYCG 1 cut(s) 37
BstNI CCWGG 3 cut(s) 831, 1061, 1328
BstNSI RCATGY 3 cut(s) 366, 679, 977
BstSCI CCNGG 3 cut(s) 829, 1059, 1326
BstSFI CTRYAG 3 cut(s) 489, 765, 944
BstV1I GCAGC 3 cut(s) 281, 666, 998
BstV2I GAAGAC 1 cut(s) 268
BstX2I RGATCY 3 cut(s) 509, 1202, 1228
BstYI RGATCY 3 cut(s) 509, 1202, 1228
BstZ17I GTATAC 1 cut(s) 673
BsuRI GGCC 5 cut(s) 82, 473, 570, 693, 1069
BtgZI GCGATG 2 cut(s) 31, 1030
BtsCI GGATG 3 cut(s) 95, 318, 931
BtsI GCAGTG 4 cut(s) 46, 664, 789, 1368
BtsIMutI CAGTG 4 cut(s) 46, 664, 789, 1368
Cac8I GCNNGC 5 cut(s) 364, 404, 418, 717, 975
CaiI CAGNNNCTG 2 cut(s) 845, 1231
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 2 cut(s) 469, 715
Cfr13I GGNCC 3 cut(s) 81, 467, 568
Csp6I GTAC 2 cut(s) 801, 1297
CviAII CATG 9 cut(s) 262, 363, 389, 423, 437, 464, 535, 676, 974
CviQI GTAC 2 cut(s) 801, 1297
DdeI CTNAG 3 cut(s) 20, 819, 990
DinI GGCGCC 1 cut(s) 129
DpnI GATC 7 cut(s) 11, 384, 511, 579, 1204, 1230, 1272
DpnII GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 262
Eco88I CYCGRG 2 cut(s) 513, 740
EcoNI CCTNNNNNAGG 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 829, 1059, 1326
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 727
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 9 cut(s) 265, 366, 392, 426, 440, 467, 538, 679, 977
FalI AAGNNNNNCTT 6 cut(s) 70, 102, 390, 422, 606, 638
FaqI GGGAC 1 cut(s) 447
FatI CATG 9 cut(s) 261, 362, 388, 422, 436, 463, 534, 675, 973
FauI CCCGC 1 cut(s) 130
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 7 cut(s) 165, 238, 270, 655, 816, 987, 1070
FokI GGATG 3 cut(s) 82, 305, 938
Fsp4HI GCNGC 7 cut(s) 165, 238, 270, 655, 816, 987, 1070
FspBI CTAG 3 cut(s) 417, 1274, 1290
GlaI GCGC 1 cut(s) 129
GluI GCNGC 7 cut(s) 165, 238, 270, 655, 816, 987, 1070
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 5 cut(s) 82, 473, 570, 693, 1069
HapII CCGG 3 cut(s) 336, 470, 716
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 9 cut(s) 265, 366, 392, 426, 440, 467, 538, 679, 977
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 4 cut(s) 743, 761, 836, 1256
HpaII CCGG 3 cut(s) 336, 470, 716
HphI GGTGA 6 cut(s) 16, 292, 350, 461, 644, 835
Hpy166II GTNNAC 5 cut(s) 143, 341, 673, 789, 1042
Hpy188I TCNGA 4 cut(s) 185, 382, 910, 1247
Hpy188III TCNNGA 3 cut(s) 513, 932, 1290
Hpy8I GTNNAC 5 cut(s) 143, 341, 673, 789, 1042
HpyAV CCTTC 5 cut(s) 224, 264, 313, 1059, 1287
HpyCH4III ACNGT 5 cut(s) 551, 560, 608, 707, 1172
HpyF3I CTNAG 3 cut(s) 20, 819, 990
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 9 cut(s) 265, 366, 392, 426, 440, 467, 538, 679, 977
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 715
KroNI GCCGGC 1 cut(s) 717
Kzo9I GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
LmnI GCTCC 2 cut(s) 524, 965
Lsp1109I GCAGC 3 cut(s) 281, 666, 998
LweI GCATC 2 cut(s) 200, 988
MaeI CTAG 3 cut(s) 417, 1274, 1290
MaeIII GTNAC 1 cut(s) 1313
MalI GATC 7 cut(s) 11, 384, 511, 579, 1204, 1230, 1272
MboI GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
MboII GAAGA 5 cut(s) 78, 81, 84, 268, 1007
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 3 cut(s) 509, 1202, 1228
MluCI AATT 6 cut(s) 73, 179, 311, 504, 855, 1301
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 2 cut(s) 752, 770
MmeI TCCRAC 2 cut(s) 246, 502
Mph1103I ATGCAT 1 cut(s) 727
MroNI GCCGGC 1 cut(s) 715
MslI CAYNNNNRTG 1 cut(s) 1049
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 3 cut(s) 336, 470, 716
MspR9I CCNGG 3 cut(s) 831, 1061, 1328
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 3 cut(s) 831, 1061, 1328
NaeI GCCGGC 1 cut(s) 717
NarI GGCGCC 1 cut(s) 128
NdeII GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
NgoMIV GCCGGC 1 cut(s) 715
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 9 cut(s) 265, 366, 392, 426, 440, 467, 538, 679, 977
NlaIV GGNNCC 3 cut(s) 129, 285, 904
NmeAIII GCCGAG 1 cut(s) 619
NsiI ATGCAT 1 cut(s) 727
NspI RCATGY 3 cut(s) 366, 679, 977
PaeI GCATGC 2 cut(s) 366, 977
PaeR7I CTCGAG 1 cut(s) 513
PasI CCCWGGG 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 693
PciI ACATGT 1 cut(s) 675
PctI GAATGC 1 cut(s) 1322
PdiI GCCGGC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 836, 1256
PfoI TCCNGGA 1 cut(s) 1326
PkrI GCNGC 7 cut(s) 166, 239, 271, 656, 817, 988, 1071
PleI GAGTC 2 cut(s) 751, 769
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 2 cut(s) 751, 769
PscI ACATGT 1 cut(s) 675
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 3 cut(s) 829, 1059, 1326
PspGI CCWGG 3 cut(s) 829, 1059, 1326
PspN4I GGNNCC 3 cut(s) 129, 285, 904
PspPI GGNCC 3 cut(s) 81, 467, 568
PstI CTGCAG 2 cut(s) 769, 948
PstNI CAGNNNCTG 2 cut(s) 845, 1231
PsuI RGATCY 3 cut(s) 509, 1202, 1228
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 2 cut(s) 802, 1298
RsaNI GTAC 2 cut(s) 801, 1297
RseI CAYNNNNRTG 1 cut(s) 1049
SatI GCNGC 7 cut(s) 165, 238, 270, 655, 816, 987, 1070
Sau3AI GATC 7 cut(s) 9, 382, 509, 577, 1202, 1228, 1270
Sau96I GGNCC 3 cut(s) 81, 467, 568
SchI GAGTC 2 cut(s) 752, 770
ScrFI CCNGG 3 cut(s) 831, 1061, 1328
SfaNI GCATC 2 cut(s) 200, 988
SfcI CTRYAG 3 cut(s) 489, 765, 944
SfoI GGCGCC 1 cut(s) 129
Sfr274I CTCGAG 1 cut(s) 513
SinI GGWCC 1 cut(s) 467
SlaI CTCGAG 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 1049
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
SphI GCATGC 2 cut(s) 366, 977
Sse9I AATT 6 cut(s) 73, 179, 311, 504, 855, 1301
SseBI AGGCCT 1 cut(s) 693
SspDI GGCGCC 1 cut(s) 127
SspMI CTAG 3 cut(s) 417, 1274, 1290
StuI AGGCCT 1 cut(s) 693
StyD4I CCNGG 3 cut(s) 829, 1059, 1326
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 5 cut(s) 551, 560, 608, 707, 1172
TaqI TCGA 4 cut(s) 306, 459, 514, 759
TaqII GACCGA 1 cut(s) 23
TasI AATT 6 cut(s) 73, 179, 311, 504, 855, 1301
TauI GCSGC 4 cut(s) 167, 240, 818, 1072
TfiI GAWTC 2 cut(s) 836, 1256
TscAI CASTG 4 cut(s) 46, 664, 789, 1368
TseI GCWGC 3 cut(s) 269, 654, 986
TspDTI ATGAA 4 cut(s) 84, 410, 520, 951
TspGWI ACGGA 3 cut(s) 108, 1169, 1224
TspRI CASTG 4 cut(s) 46, 664, 789, 1368
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 262
XapI RAATTY 4 cut(s) 73, 179, 311, 1301
XbaI TCTAGA 1 cut(s) 1289
XceI RCATGY 3 cut(s) 366, 679, 977
XhoI CTCGAG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 672
XspI CTAG 3 cut(s) 417, 1274, 1290
Zsp2I ATGCAT 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.