MD00G1129200.v1.1

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010272
Physical Location & Seq
Reverse (-)
27490320 .. 27496062
5743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1129200.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGTGATGATCCGCAATCTCAGTCGCAGTCTCAGTCGCAGTGCCATCGCCAAAACACTCCTCTCGCCATCAAATTCAAGGCCCTCTTCCCATTCTGCTATTCCGTTGTTGGCGCCGTTGGCGGGTCAACGCCTACGCAACAACTCTGCCGCATTTGAACCCGAATTCAGAGATACCACCACCACCAGATGCCTTCACTCGCTTGGCGGCGGTGGCGGACTGCCTTCCTCCATGAGGGCAGCTGTCTTCAGGGAACCCAACAAACCTCTCACCTTCGAGGAATTTCACATCCCTCGCCCCAAATCCGGTGAACTTCTCATCAAAACCAAGGCATGCGGAGTTTGCCACTCTGATCTCCATGTTATGAAGGGCGAGCTTCCATTTGCTAGCCCATGTGTTGTGGGACATGAGATAACTGGTGAGGTTGTCGAACATGGACCGGCCACAGACACCAAAACTAAAGCAAGATTACCAATTGGATCTCGAGTTGTTGGAGCTTTCATCATGCCTTGCGGCAACTGTTCCTACTGTTCAAAGGGCCATGATGATCTATGCGAGGATTTCTTTGCCTATAACCGTGCAAAAGGAACTCTTTATGATGGTGAAACTCGGCTGTTCCTCCGCAGCAGTGGAAAACCAGTATACATGTATAGTATGGGAGGCCTTGCTGAATACTGTATTGTGCCGGCAAATGCATTGACTATTTTGCCCGAGTCATTGCCATACATCGAGTCTGCAGTTTTAGGTTGTGCAGTGTTTACTGCTTATGGTACTATGGCTAATGCGGCTCAGGTGAAGCCTGGGGATTCAGTTGCTGTGATCGGAATTGGGGGTGTAGGGTCAAGCTGTTTGCAGATAGCAAGAGCATTTGGTGCCTCTGATATTATCGCTGTGGATGTTCAAGATGAAAAACTGCAGAAAGCCAAGATATTTGGAGCTACGCATGCTGTAAATGCAGCTAAAGAAGATGCTGTTGCCAAAATAATGGAAATAACTGGTGGAAAGGGTGTGGACATCGCTGTGGAAGCCCTGGGAAGGCCGCAGACATTTTTGCAATGTGTGCAAAGTGTGAAAGATGGAGGAAAAGCTGTAATGGTTGGGCTTTCACAAGCTGGTGCAGTTGGGGAAGTAGATATAAACCGTCTTGTCCGTAGAAAGATACAAATCATTGGATCTTACGGAGGGAGGGCAAGGCAGGATCTGCCAAAGTTGGTCAGATTGGCAGAATCAGGCATATTTGATCTAGCTAATGCTGTTTCTAGAAGGTACAAGTTTGAGGAGAGTAACGAAGCATTCCAGGAACTCAATAAGGGAAGCATTGTTGGTCGGGCAGTGGTTGAGATAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

450

Amino Acids

47.79

Weight (kDa)

8.83

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 102 - 237 4e-24 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 263 - 436 3.4e-07 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 278 - 409 1.4e-33 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 311 - 446 9.2e-13 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 872
AccI GTMKAC 1 cut(s) 642
AclWI GGATC 4 cut(s) 4, 487, 1180, 1206
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 3 cut(s) 73, 164, 281
AcuI CTGAAG 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 772, 1268
AfiI CCNNNNNNNGG 4 cut(s) 122, 234, 305, 1035
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 4 cut(s) 78, 158, 534, 902
AjnI CCWGG 3 cut(s) 799, 1029, 1296
AluBI AGCT 9 cut(s) 242, 376, 497, 846, 938, 959, 1088, 1112, 1247
AluI AGCT 9 cut(s) 242, 376, 497, 846, 938, 959, 1088, 1112, 1247
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 4 cut(s) 4, 487, 1180, 1206
AlwNI CAGNNNCTG 2 cut(s) 815, 1201
Ama87I CYCGRG 2 cut(s) 483, 710
AoxI GGCC 5 cut(s) 80, 441, 538, 661, 1037
ApeKI GCWGC 3 cut(s) 239, 624, 956
ApoI RAATTY 3 cut(s) 73, 164, 281
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 3 cut(s) 81, 437, 538
AsuHPI GGTGA 6 cut(s) 16, 262, 320, 431, 614, 805
AsuNHI GCTAGC 1 cut(s) 386
AvaI CYCGRG 2 cut(s) 483, 710
AvaII GGWCC 1 cut(s) 437
BanI GGYRCC 2 cut(s) 112, 872
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 3 cut(s) 251, 636, 968
BccI CCATC 4 cut(s) 53, 76, 593, 1070
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 3 cut(s) 801, 1031, 1298
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 3 cut(s) 387, 1244, 1260
BfmI CTRYAG 2 cut(s) 735, 914
BfoI RGCGCY 1 cut(s) 116
BisI GCNGC 8 cut(s) 150, 208, 240, 514, 625, 786, 957, 1040
BlsI GCNGC 8 cut(s) 151, 209, 241, 515, 626, 787, 958, 1041
Bme1390I CCNGG 3 cut(s) 801, 1031, 1298
Bme18I GGWCC 1 cut(s) 437
BmeT110I CYCGRG 2 cut(s) 483, 710
BmgT120I GGNCC 3 cut(s) 81, 437, 538
BmiI GGNNCC 3 cut(s) 114, 255, 874
BmrFI CCNGG 3 cut(s) 801, 1031, 1298
BmsI GCATC 2 cut(s) 179, 958
BmtI GCTAGC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 789
BsaBI GATNNNNATC 1 cut(s) 1163
BsaHI GRCGYC 1 cut(s) 113
BsaJI CCNNGG 4 cut(s) 327, 800, 1029, 1030
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 234, 305, 1035
Bse118I RCCGGY 2 cut(s) 439, 685
Bse1I ACTGG 3 cut(s) 421, 638, 1000
Bse3DI GCAATG 2 cut(s) 716, 1061
Bse8I GATNNNNATC 1 cut(s) 1163
BseBI CCWGG 3 cut(s) 801, 1031, 1298
BseDI CCNNGG 4 cut(s) 327, 800, 1029, 1030
BseGI GGATG 2 cut(s) 288, 901
BseJI GATNNNNATC 1 cut(s) 1163
BseLI CCNNNNNNNGG 4 cut(s) 122, 234, 305, 1035
BseMI GCAATG 2 cut(s) 716, 1061
BseMII CTCAG 3 cut(s) 34, 46, 803
BseNI ACTGG 3 cut(s) 421, 638, 1000
BseRI GAGGAG 2 cut(s) 50, 1292
BseXI GCAGC 3 cut(s) 251, 636, 968
BsgI GTGCAG 2 cut(s) 771, 1137
BshFI GGCC 5 cut(s) 82, 443, 540, 663, 1039
BshNI GGYRCC 2 cut(s) 112, 872
BsiHKCI CYCGRG 2 cut(s) 483, 710
BsiSI CCGG 3 cut(s) 306, 440, 686
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 4 cut(s) 122, 234, 305, 1035
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 1292
BsnI GGCC 5 cut(s) 82, 443, 540, 663, 1039
BsoBI CYCGRG 2 cut(s) 483, 710
Bsp143I GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
BspANI GGCC 5 cut(s) 82, 443, 540, 663, 1039
BspCNI CTCAG 3 cut(s) 33, 45, 802
BspLI GGNNCC 3 cut(s) 114, 255, 874
BspMAI CTGCAG 2 cut(s) 739, 918
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 4 cut(s) 4, 487, 1180, 1206
BspT107I GGYRCC 2 cut(s) 112, 872
BsrDI GCAATG 2 cut(s) 716, 1061
BsrFI RCCGGY 2 cut(s) 439, 685
BsrI ACTGG 3 cut(s) 421, 638, 1000
BssAI RCCGGY 2 cut(s) 439, 685
BssECI CCNNGG 4 cut(s) 327, 800, 1029, 1030
BssMI GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
BssNAI GTATAC 1 cut(s) 643
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 327
Bst1107I GTATAC 1 cut(s) 643
Bst2UI CCWGG 3 cut(s) 801, 1031, 1298
Bst4CI ACNGT 5 cut(s) 521, 530, 578, 677, 1142
Bst6I CTCTTC 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 113
BstAPI GCANNNNNTGC 3 cut(s) 872, 1060, 1201
BstC8I GCNNGC 5 cut(s) 334, 374, 388, 687, 945
BstDEI CTNAG 3 cut(s) 20, 32, 789
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 288, 901
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 8 cut(s) 12, 355, 482, 550, 822, 1175, 1201, 1243
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
BstNI CCWGG 3 cut(s) 801, 1031, 1298
BstNSI RCATGY 3 cut(s) 336, 649, 947
BstSCI CCNGG 3 cut(s) 799, 1029, 1296
BstSFI CTRYAG 2 cut(s) 735, 914
BstV1I GCAGC 3 cut(s) 251, 636, 968
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 479, 1172, 1198
BstXI CCANNNNNNTGG 1 cut(s) 985
BstYI RGATCY 3 cut(s) 479, 1172, 1198
BstZ17I GTATAC 1 cut(s) 643
BsuRI GGCC 5 cut(s) 82, 443, 540, 663, 1039
BtgZI GCGATG 2 cut(s) 31, 1000
BtsCI GGATG 2 cut(s) 288, 901
BtsI GCAGTG 4 cut(s) 46, 634, 759, 1338
BtsIMutI CAGTG 4 cut(s) 46, 634, 759, 1338
Cac8I GCNNGC 5 cut(s) 334, 374, 388, 687, 945
CaiI CAGNNNCTG 2 cut(s) 815, 1201
CfoI GCGC 1 cut(s) 115
Cfr10I RCCGGY 2 cut(s) 439, 685
Cfr13I GGNCC 3 cut(s) 81, 437, 538
Csp6I GTAC 2 cut(s) 771, 1267
CviQI GTAC 2 cut(s) 771, 1267
DdeI CTNAG 3 cut(s) 20, 32, 789
DinI GGCGCC 1 cut(s) 114
DpnI GATC 8 cut(s) 11, 354, 481, 549, 821, 1174, 1200, 1242
DpnII GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 327
Eco147I AGGCCT 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 232
Eco88I CYCGRG 2 cut(s) 483, 710
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 3 cut(s) 799, 1029, 1296
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 697
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 327
FalI AAGNNNNNCTT 4 cut(s) 70, 102, 576, 608
FaqI GGGAC 1 cut(s) 417
FauI CCCGC 1 cut(s) 115
FblI GTMKAC 1 cut(s) 642
Fnu4HI GCNGC 8 cut(s) 150, 208, 240, 514, 625, 786, 957, 1040
FokI GGATG 2 cut(s) 275, 908
Fsp4HI GCNGC 8 cut(s) 150, 208, 240, 514, 625, 786, 957, 1040
FspBI CTAG 3 cut(s) 387, 1244, 1260
GlaI GCGC 1 cut(s) 114
GluI GCNGC 8 cut(s) 150, 208, 240, 514, 625, 786, 957, 1040
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 5 cut(s) 82, 443, 540, 663, 1039
HapII CCGG 3 cut(s) 306, 440, 686
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HinfI GANTC 4 cut(s) 713, 731, 806, 1226
HpaII CCGG 3 cut(s) 306, 440, 686
HphI GGTGA 6 cut(s) 16, 262, 320, 431, 614, 805
Hpy166II GTNNAC 5 cut(s) 128, 311, 643, 759, 1012
Hpy188I TCNGA 5 cut(s) 170, 352, 824, 880, 1217
Hpy188III TCNNGA 3 cut(s) 483, 902, 1260
Hpy8I GTNNAC 5 cut(s) 128, 311, 643, 759, 1012
HpyAV CCTTC 6 cut(s) 203, 234, 283, 361, 1029, 1257
HpyCH4III ACNGT 5 cut(s) 521, 530, 578, 677, 1142
HpyF3I CTNAG 3 cut(s) 20, 32, 789
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 1 cut(s) 113
KasI GGCGCC 1 cut(s) 112
KroI GCCGGC 1 cut(s) 685
KroNI GCCGGC 1 cut(s) 687
Kzo9I GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
LmnI GCTCC 2 cut(s) 494, 935
Lsp1109I GCAGC 3 cut(s) 251, 636, 968
LweI GCATC 2 cut(s) 179, 958
MaeI CTAG 3 cut(s) 387, 1244, 1260
MaeIII GTNAC 1 cut(s) 1283
MalI GATC 8 cut(s) 11, 354, 481, 549, 821, 1174, 1200, 1242
MboI GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
MboII GAAGA 3 cut(s) 78, 238, 977
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 3 cut(s) 479, 1172, 1198
MluCI AATT 5 cut(s) 73, 164, 281, 474, 825
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 2 cut(s) 722, 740
MmeI TCCRAC 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 697
MroNI GCCGGC 1 cut(s) 685
MslI CAYNNNNRTG 1 cut(s) 1019
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 3 cut(s) 306, 440, 686
MspR9I CCNGG 3 cut(s) 801, 1031, 1298
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 1292
MvaI CCWGG 3 cut(s) 801, 1031, 1298
NaeI GCCGGC 1 cut(s) 687
NarI GGCGCC 1 cut(s) 113
NdeII GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
NgoMIV GCCGGC 1 cut(s) 685
NheI GCTAGC 1 cut(s) 386
NlaIV GGNNCC 3 cut(s) 114, 255, 874
NmeAIII GCCGAG 1 cut(s) 589
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 3 cut(s) 336, 649, 947
PaeI GCATGC 2 cut(s) 336, 947
PaeR7I CTCGAG 1 cut(s) 483
PasI CCCWGGG 1 cut(s) 1030
PceI AGGCCT 1 cut(s) 663
PciI ACATGT 1 cut(s) 645
PctI GAATGC 1 cut(s) 1292
PdiI GCCGGC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 806, 1226
PfoI TCCNGGA 1 cut(s) 1296
PkrI GCNGC 8 cut(s) 151, 209, 241, 515, 626, 787, 958, 1041
PleI GAGTC 2 cut(s) 721, 739
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 2 cut(s) 721, 739
PscI ACATGT 1 cut(s) 645
Psp6I CCWGG 3 cut(s) 799, 1029, 1296
PspGI CCWGG 3 cut(s) 799, 1029, 1296
PspN4I GGNNCC 3 cut(s) 114, 255, 874
PspPI GGNCC 3 cut(s) 81, 437, 538
PstI CTGCAG 2 cut(s) 739, 918
PstNI CAGNNNCTG 2 cut(s) 815, 1201
PsuI RGATCY 3 cut(s) 479, 1172, 1198
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 2 cut(s) 772, 1268
RsaNI GTAC 2 cut(s) 771, 1267
RseI CAYNNNNRTG 1 cut(s) 1019
SatI GCNGC 8 cut(s) 150, 208, 240, 514, 625, 786, 957, 1040
Sau3AI GATC 8 cut(s) 9, 352, 479, 547, 819, 1172, 1198, 1240
Sau96I GGNCC 3 cut(s) 81, 437, 538
SchI GAGTC 2 cut(s) 722, 740
ScrFI CCNGG 3 cut(s) 801, 1031, 1298
SfaNI GCATC 2 cut(s) 179, 958
SfcI CTRYAG 2 cut(s) 735, 914
SfoI GGCGCC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 483
SinI GGWCC 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 1019
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SphI GCATGC 2 cut(s) 336, 947
Sse9I AATT 5 cut(s) 73, 164, 281, 474, 825
SseBI AGGCCT 1 cut(s) 663
SspDI GGCGCC 1 cut(s) 112
SspMI CTAG 3 cut(s) 387, 1244, 1260
StuI AGGCCT 1 cut(s) 663
StyD4I CCNGG 3 cut(s) 799, 1029, 1296
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 5 cut(s) 521, 530, 578, 677, 1142
TaqI TCGA 4 cut(s) 276, 429, 484, 729
TasI AATT 5 cut(s) 73, 164, 281, 474, 825
TauI GCSGC 5 cut(s) 152, 210, 516, 788, 1042
TfiI GAWTC 2 cut(s) 806, 1226
TscAI CASTG 4 cut(s) 46, 634, 759, 1338
TseI GCWGC 3 cut(s) 239, 624, 956
TspDTI ATGAA 3 cut(s) 380, 490, 921
TspGWI ACGGA 3 cut(s) 93, 1139, 1194
TspRI CASTG 4 cut(s) 46, 634, 759, 1338
VpaK11BI GGWCC 1 cut(s) 437
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 3 cut(s) 73, 164, 281
XbaI TCTAGA 1 cut(s) 1259
XceI RCATGY 3 cut(s) 336, 649, 947
XhoI CTCGAG 1 cut(s) 483
XmiI GTMKAC 1 cut(s) 642
XspI CTAG 3 cut(s) 387, 1244, 1260
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.