RLG00000018760

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010272
Physical Location & Seq
Reverse (-)
35509638 .. 35516136
6499 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018760

Sequence Viewer

Length: 1119 bp
ATGAGAGCAGCCGTCTTCTGGGAACCAAACAAACCTCTCTCCATTGAAGAATTCCACATCCCTCGCCCCAAATCCGGTGAAATTCTCATCAAAACCAAGGCCTGTGGAGTCTGCCATTCTGATCTCCATGTTTTGAAGGGTGAGCTTCCATTTGCTAGCCCTTGTGTCGTGGGGCACGAGATTACTGGCGAGGTCGTCGAACATGGACCCGCTACAGACAAAAAGATCACTGAAAGATTACCGATTGGATCTAATGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTTCTTCTGTTCAAAGGGCCACGATGATTTATGTGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGCAGCAGTGGAAAACCAGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGGATTGGCTATTTTACCGAAGTCATTGCCATATACAGAGTCTGCCATTTTAGGCTGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGCGGCTGAGGTTCATCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGTGTTGGATCAAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGACATTATTGCAGTCGATATTCAAGACGACAAACTGCAGAAAGCTAAGATATTTGGTGCTACTCATGTTATAAATGCGGCTAAAGAAGATGCTGTTGAAAAATTATTGGAAATAACTGGTGGGAAAGGTGTGGACGTTGCTGTGGAAGCCCTGGGAAAACCACAGACCTTTTTGCAATGCACGCAAAGTGTGAGAGATGGAGGAAAAGCTGTCTTCATCGGGCTTACAGCAGCCGGAGCAATTGGGGAAGTAGATATTAACCGTCTTGTTCGTAGAAAGATAAAAATCATTGGATCATACGGAGGAAGGGCAAGGCAGGATCTTCCAAAGATAGTTAGACTAGCAGAAAGTGGTATATTTGATCTCAACAGTGCTGTTTCAAGAAGATACAAATTCGAGGAGTCTGACACTGCATTCCAGGATCTCAACCAGGGAAAAATTGTTGGTCGGGCAGTGGTTGAGATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

373

Amino Acids

39.73

Weight (kDa)

6.76

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 25 - 160 5.3e-23 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 183 - 359 1.2e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 201 - 332 3.5e-33 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 234 - 369 1.6e-14 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 1 cut(s) 411
AciI CCGC 4 cut(s) 210, 391, 554, 725
AclWI GGATC 5 cut(s) 256, 616, 949, 975, 1077
AcsI RAATTY 3 cut(s) 50, 81, 1040
AfaI GTAC 2 cut(s) 443, 453
AfiI CCNNNNNNNGG 3 cut(s) 18, 74, 461
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 6 cut(s) 47, 136, 303, 671, 746, 1029
AjnI CCWGG 4 cut(s) 568, 798, 1065, 1077
AluBI AGCT 6 cut(s) 145, 266, 288, 615, 692, 857
AluI AGCT 6 cut(s) 145, 266, 288, 615, 692, 857
AlwI GGATC 5 cut(s) 256, 616, 949, 975, 1077
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 3 cut(s) 99, 307, 430
ApeKI GCWGC 3 cut(s) 8, 393, 878
ApoI RAATTY 3 cut(s) 50, 81, 1040
AspS9I GGNCC 2 cut(s) 206, 307
AsuHPI GGTGA 3 cut(s) 89, 152, 383
AsuNHI GCTAGC 1 cut(s) 155
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 641
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 2 cut(s) 7, 853
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 3 cut(s) 20, 405, 890
BccI CCATC 2 cut(s) 362, 839
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 638, 672
BciT130I CCWGG 4 cut(s) 570, 800, 1067, 1079
BfaI CTAG 2 cut(s) 156, 989
BfmI CTRYAG 3 cut(s) 213, 620, 683
BisI GCNGC 5 cut(s) 9, 394, 555, 726, 879
BlsI GCNGC 5 cut(s) 10, 395, 556, 727, 880
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 4 cut(s) 570, 800, 1067, 1079
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 206, 307
BmiI GGNNCC 4 cut(s) 24, 208, 358, 643
BmrFI CCNGG 4 cut(s) 570, 800, 1067, 1079
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 727
BmtI GCTAGC 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 7, 853
Bpu10I CCTNAGC 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 932
BsaJI CCNNGG 5 cut(s) 96, 569, 798, 799, 1078
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 74, 461
Bse1I ACTGG 4 cut(s) 190, 407, 601, 769
Bse3DI GCAATG 2 cut(s) 485, 830
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 4 cut(s) 570, 800, 1067, 1079
BseDI CCNNGG 5 cut(s) 96, 569, 798, 799, 1078
BseGI GGATG 2 cut(s) 57, 565
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 3 cut(s) 18, 74, 461
BseMI GCAATG 2 cut(s) 485, 830
BseMII CTCAG 2 cut(s) 429, 549
BseNI ACTGG 4 cut(s) 190, 407, 601, 769
BseRI GAGGAG 1 cut(s) 1061
BseSI GKGCMC 1 cut(s) 177
BseXI GCAGC 3 cut(s) 20, 405, 890
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 3 cut(s) 101, 309, 432
BshNI GGYRCC 1 cut(s) 641
BsiSI CCGG 2 cut(s) 75, 882
BslI CCNNNNNNNGG 3 cut(s) 18, 74, 461
BsmI GAATGC 1 cut(s) 1061
BsnI GGCC 3 cut(s) 101, 309, 432
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
BspACI CCGC 4 cut(s) 210, 391, 554, 725
BspANI GGCC 3 cut(s) 101, 309, 432
BspCNI CTCAG 2 cut(s) 430, 550
BspLI GGNNCC 4 cut(s) 24, 208, 358, 643
BspMAI CTGCAG 2 cut(s) 624, 687
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 5 cut(s) 256, 616, 949, 975, 1077
BspT107I GGYRCC 1 cut(s) 641
BsrDI GCAATG 2 cut(s) 485, 830
BsrI ACTGG 4 cut(s) 190, 407, 601, 769
BssECI CCNNGG 5 cut(s) 96, 569, 798, 799, 1078
BssMI GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
BssNAI GTATAC 1 cut(s) 412
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 96
Bst1107I GTATAC 1 cut(s) 412
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 4 cut(s) 570, 800, 1067, 1079
Bst4CI ACNGT 4 cut(s) 347, 446, 911, 1019
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 157, 830
BstDEI CTNAG 3 cut(s) 438, 558, 693
BstF5I GGATG 2 cut(s) 57, 565
BstKTI GATC 8 cut(s) 124, 228, 251, 611, 944, 970, 1012, 1072
BstMBI GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
BstMWI GCNNNNNNNGC 6 cut(s) 539, 554, 641, 794, 829, 884
BstNI CCWGG 4 cut(s) 570, 800, 1067, 1079
BstNSI RCATGY 2 cut(s) 418, 554
BstSCI CCNGG 4 cut(s) 568, 798, 1065, 1077
BstSFI CTRYAG 3 cut(s) 213, 620, 683
BstSLI GKGCMC 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 20, 405, 890
BstV2I GAAGAC 2 cut(s) 7, 853
BstX2I RGATCY 3 cut(s) 248, 967, 1069
BstYI RGATCY 3 cut(s) 248, 967, 1069
BstZ17I GTATAC 1 cut(s) 412
BsuRI GGCC 3 cut(s) 101, 309, 432
BtsCI GGATG 2 cut(s) 57, 565
BtsI GCAGTG 3 cut(s) 403, 1056, 1107
BtsIMutI CAGTG 5 cut(s) 228, 403, 1024, 1056, 1107
Cac8I GCNNGC 2 cut(s) 157, 830
CaiI CAGNNNCTG 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 206, 307
Csp6I GTAC 2 cut(s) 442, 452
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 6 cut(s) 128, 203, 274, 415, 551, 713
CviQI GTAC 2 cut(s) 442, 452
DdeI CTNAG 3 cut(s) 438, 558, 693
DpnI GATC 8 cut(s) 123, 227, 250, 610, 943, 969, 1011, 1071
DpnII GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
Eco130I CCWWGG 1 cut(s) 96
Eco147I AGGCCT 2 cut(s) 101, 432
Eco47I GGWCC 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 4 cut(s) 568, 798, 1065, 1077
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 6 cut(s) 131, 206, 277, 418, 554, 716
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FatI CATG 6 cut(s) 127, 202, 273, 414, 550, 712
FauI CCCGC 1 cut(s) 217
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 5 cut(s) 9, 394, 555, 726, 879
FokI GGATG 2 cut(s) 44, 552
Fsp4HI GCNGC 5 cut(s) 9, 394, 555, 726, 879
FspBI CTAG 2 cut(s) 156, 989
GluI GCNGC 5 cut(s) 9, 394, 555, 726, 879
HaeIII GGCC 3 cut(s) 101, 309, 432
HapII CCGG 2 cut(s) 75, 882
Hin1II CATG 6 cut(s) 131, 206, 277, 418, 554, 716
HinfI GANTC 4 cut(s) 108, 500, 575, 1049
HpaII CCGG 2 cut(s) 75, 882
HphI GGTGA 3 cut(s) 89, 152, 383
Hpy166II GTNNAC 2 cut(s) 412, 781
Hpy188I TCNGA 3 cut(s) 121, 649, 1054
Hpy188III TCNNGA 2 cut(s) 671, 1029
Hpy8I GTNNAC 2 cut(s) 412, 781
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 2 cut(s) 130, 948
HpyCH4III ACNGT 4 cut(s) 347, 446, 911, 1019
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 7 cut(s) 521, 622, 659, 685, 823, 828, 1061
HpyF10VI GCNNNNNNNGC 6 cut(s) 539, 554, 641, 794, 829, 884
HpyF3I CTNAG 3 cut(s) 438, 558, 693
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 6 cut(s) 131, 206, 277, 418, 554, 716
Kzo9I GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
LmnI GCTCC 2 cut(s) 263, 884
Lsp1109I GCAGC 3 cut(s) 20, 405, 890
LweI GCATC 1 cut(s) 727
MaeI CTAG 2 cut(s) 156, 989
MaeII ACGT 1 cut(s) 783
MalI GATC 8 cut(s) 123, 227, 250, 610, 943, 969, 1011, 1071
MboI GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
MboII GAAGA 7 cut(s) 7, 59, 286, 746, 853, 962, 1044
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 3 cut(s) 248, 967, 1069
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 6 cut(s) 50, 81, 749, 888, 1040, 1086
MlyI GAGTC 3 cut(s) 117, 509, 1058
MmeI TCCRAC 3 cut(s) 241, 586, 672
MseI TTAA 1 cut(s) 906
MspI CCGG 2 cut(s) 75, 882
MspR9I CCNGG 4 cut(s) 570, 800, 1067, 1079
MunI CAATTG 1 cut(s) 888
Mva1269I GAATGC 1 cut(s) 1061
MvaI CCWGG 4 cut(s) 570, 800, 1067, 1079
MwoI GCNNNNNNNGC 6 cut(s) 539, 554, 641, 794, 829, 884
NdeII GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 6 cut(s) 131, 206, 277, 418, 554, 716
NlaIV GGNNCC 4 cut(s) 24, 208, 358, 643
NspI RCATGY 2 cut(s) 418, 554
PasI CCCWGGG 1 cut(s) 799
PceI AGGCCT 2 cut(s) 101, 432
PciI ACATGT 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 1061
PfeI GAWTC 1 cut(s) 575
PflMI CCANNNNNTGG 1 cut(s) 461
PfoI TCCNGGA 1 cut(s) 1065
PkrI GCNGC 5 cut(s) 10, 395, 556, 727, 880
PleI GAGTC 3 cut(s) 116, 508, 1057
PpsI GAGTC 3 cut(s) 116, 508, 1057
PscI ACATGT 1 cut(s) 414
PsiI TTATAA 1 cut(s) 719
Psp6I CCWGG 4 cut(s) 568, 798, 1065, 1077
PspGI CCWGG 4 cut(s) 568, 798, 1065, 1077
PspN4I GGNNCC 4 cut(s) 24, 208, 358, 643
PspPI GGNCC 2 cut(s) 206, 307
PstI CTGCAG 2 cut(s) 624, 687
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 3 cut(s) 248, 967, 1069
RsaI GTAC 2 cut(s) 443, 453
RsaNI GTAC 2 cut(s) 442, 452
SaqAI TTAA 1 cut(s) 906
SatI GCNGC 5 cut(s) 9, 394, 555, 726, 879
Sau3AI GATC 8 cut(s) 121, 225, 248, 608, 941, 967, 1009, 1069
Sau96I GGNCC 2 cut(s) 206, 307
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 3 cut(s) 117, 509, 1058
ScrFI CCNGG 4 cut(s) 570, 800, 1067, 1079
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 1 cut(s) 727
SfcI CTRYAG 3 cut(s) 213, 620, 683
SinI GGWCC 1 cut(s) 206
Sse9I AATT 6 cut(s) 50, 81, 749, 888, 1040, 1086
SseBI AGGCCT 2 cut(s) 101, 432
SsiI CCGC 4 cut(s) 210, 391, 554, 725
SspMI CTAG 2 cut(s) 156, 989
StuI AGGCCT 2 cut(s) 101, 432
StyD4I CCNGG 4 cut(s) 568, 798, 1065, 1077
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 4 cut(s) 347, 446, 911, 1019
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 198, 663, 1044
TasI AATT 6 cut(s) 50, 81, 749, 888, 1040, 1086
TatI WGTACW 1 cut(s) 441
TauI GCSGC 2 cut(s) 557, 728
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 1 cut(s) 906
Tru9I TTAA 1 cut(s) 906
TscAI CASTG 5 cut(s) 235, 403, 1024, 1063, 1107
TseI GCWGC 3 cut(s) 8, 393, 878
TspDTI ATGAA 2 cut(s) 554, 853
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 5 cut(s) 235, 403, 1024, 1063, 1107
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 3 cut(s) 50, 81, 1040
XceI RCATGY 2 cut(s) 418, 554
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 2 cut(s) 156, 989
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.