Rh2DG329500

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010272
Physical Location & Seq
Reverse (-)
41734908 .. 41741404
6497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG329500.1

Sequence Viewer

Length: 1062 bp
ATGATGATCCGCAGTGCCATCGCCAGAAGACTTTCCTCAAAGCCAACCTCGTCTTCATCTTCTTATGCTTGGTTGAGCACCACCACTAGTACGACGTCGTTTGACTTGGTTGATCAAAGCCTCCATGGGAATCATCAGAAGAGGAGGAGCAGCACGACCTCATTACCTGTTGGGCTGCCTTCTTACATGAGAGCAGCCGTCTTCTGGGAACCAAACAGACCTCTCTCCATTGAAGAATTCCACATCCCTCGCCCCAAATCCGGTGAAATTCTCATCAAAACCAAGGCCTGTGGAGTCTGCCATTCTGATCTCCATGTTTTGAAGGGTGAGCTTCCATTCGCTAGCCCTTGTGTCGTGGGCCACGAGATTACTGGTGAGGTCGTCGAACATGGACCCGCTACAGACAAAAAGATCACAGAAAGATTACCGATTGGATCTAATGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTTCTTCTGTTCAAAGGGCCACGACGATTTATGTGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGCAGCAGTGGAAAACCCGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGGATTGGCTATTTTACCAAAGTCATTGCCATATACAGAGTCTGCCATTTTAGGCTGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGCGGCTGAGGTTCGTCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGTGTTGGATCCAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGACATTATTGCAGTCGATATTCAAGACGACAAACTGCAGAAAGCTAAGATATTTGGTGCTACTCATGTTATAAATGCAGCTAAAGAAGATGCTGTTGAAAAATTATTGGGCTATAGAACCACTTGTAGTCCTTCCTGGAGAAGTACCAGCAGGTTCTTCCTCAAGAACGCTCCAAGTGAAGATGCAGGAAGCACTTGGATTTGTAAAGAAAAATATTTCTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

353

Amino Acids

38.22

Weight (kDa)

8.38

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 87 - 222 4.8e-23 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 263 - 313 3.6e-12 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AatII GACGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 975
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 647
AccI GTMKAC 1 cut(s) 597
AciI CCGC 4 cut(s) 10, 396, 577, 740
AclWI GGATC 3 cut(s) 442, 789, 802
AcsI RAATTY 2 cut(s) 236, 267
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 4 cut(s) 91, 629, 639, 979
AfiI CCNNNNNNNGG 3 cut(s) 204, 260, 647
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 5 cut(s) 233, 322, 489, 857, 932
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 2 cut(s) 754, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 6 cut(s) 331, 452, 474, 801, 878, 914
AluI AGCT 6 cut(s) 331, 452, 474, 801, 878, 914
Alw21I GWGCWC 1 cut(s) 80
AlwI GGATC 3 cut(s) 442, 789, 802
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 285, 358, 493, 616
ApeKI GCWGC 5 cut(s) 150, 175, 194, 579, 911
ApoI RAATTY 2 cut(s) 236, 267
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 358, 392, 493
AsuHPI GGTGA 4 cut(s) 275, 338, 386, 569
AsuNHI GCTAGC 1 cut(s) 341
AvaII GGWCC 1 cut(s) 392
BamHI GGATCC 1 cut(s) 794
BanI GGYRCC 1 cut(s) 827
BauI CACGAG 1 cut(s) 362
BbsI GAAGAC 3 cut(s) 34, 45, 193
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 5 cut(s) 162, 162, 206, 591, 923
BccI CCATC 2 cut(s) 26, 548
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 3 cut(s) 35, 824, 858
BciT130I CCWGG 2 cut(s) 756, 970
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 2 cut(s) 87, 342
BfmI CTRYAG 4 cut(s) 399, 806, 869, 946
BfuAI ACCTGC 1 cut(s) 975
BisI GCNGC 6 cut(s) 151, 176, 195, 580, 741, 912
BlsI GCNGC 6 cut(s) 152, 177, 196, 581, 742, 913
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 2 cut(s) 756, 970
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 358, 392, 493
BmiI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BmrFI CCNGG 2 cut(s) 756, 970
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 2 cut(s) 913, 1006
BmtI GCTAGC 1 cut(s) 345
BmuI ACTGGG 1 cut(s) 792
BpiI GAAGAC 3 cut(s) 34, 45, 193
BpmI CTGGAG 1 cut(s) 991
Bpu10I CCTNAGC 1 cut(s) 744
BpuEI CTTGAG 1 cut(s) 980
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 124, 282, 755
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 260, 647
Bse1I ACTGG 2 cut(s) 376, 787
Bse3DI GCAATG 1 cut(s) 671
BseBI CCWGG 2 cut(s) 756, 970
BseDI CCNNGG 3 cut(s) 124, 282, 755
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 615, 735
BseNI ACTGG 2 cut(s) 376, 787
BseRI GAGGAG 2 cut(s) 157, 160
BseXI GCAGC 5 cut(s) 162, 162, 206, 591, 923
BsgI GTGCAG 1 cut(s) 726
BshFI GGCC 4 cut(s) 287, 360, 495, 618
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BsnI GGCC 4 cut(s) 287, 360, 495, 618
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 6 cut(s) 6, 112, 307, 411, 434, 794
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 4 cut(s) 10, 396, 577, 740
BspANI GGCC 4 cut(s) 287, 360, 495, 618
BspCNI CTCAG 2 cut(s) 616, 736
BspLI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BspMAI CTGCAG 2 cut(s) 810, 873
BspMI ACCTGC 1 cut(s) 975
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 442, 789, 802
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 376, 787
BssECI CCNNGG 3 cut(s) 124, 282, 755
BssMI GATC 6 cut(s) 6, 112, 307, 411, 434, 794
BssNAI GTATAC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 2 cut(s) 124, 282
Bst1107I GTATAC 1 cut(s) 598
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 756, 970
Bst4CI ACNGT 2 cut(s) 533, 632
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 624, 744, 879
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 243
BstKTI GATC 6 cut(s) 9, 115, 310, 414, 437, 797
BstMBI GATC 6 cut(s) 6, 112, 307, 411, 434, 794
BstMWI GCNNNNNNNGC 3 cut(s) 725, 740, 827
BstNI CCWGG 2 cut(s) 756, 970
BstNSI RCATGY 2 cut(s) 604, 740
BstSCI CCNGG 2 cut(s) 754, 968
BstSFI CTRYAG 4 cut(s) 399, 806, 869, 946
BstV1I GCAGC 5 cut(s) 162, 162, 206, 591, 923
BstV2I GAAGAC 3 cut(s) 34, 45, 193
BstX2I RGATCY 2 cut(s) 434, 794
BstYI RGATCY 2 cut(s) 434, 794
BstZ17I GTATAC 1 cut(s) 598
BsuRI GGCC 4 cut(s) 287, 360, 495, 618
BtgI CCRYGG 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 4
BtsCI GGATG 1 cut(s) 243
BtsI GCAGTG 2 cut(s) 19, 589
BtsIMutI CAGTG 2 cut(s) 19, 589
BveI ACCTGC 1 cut(s) 975
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 689
Cfr13I GGNCC 3 cut(s) 358, 392, 493
Csp6I GTAC 4 cut(s) 90, 628, 638, 978
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 8 cut(s) 125, 187, 314, 389, 460, 601, 737, 899
CviQI GTAC 4 cut(s) 90, 628, 638, 978
DdeI CTNAG 3 cut(s) 624, 744, 879
DpnI GATC 6 cut(s) 8, 114, 309, 413, 436, 796
DpnII GATC 6 cut(s) 6, 112, 307, 411, 434, 794
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 2 cut(s) 124, 282
Eco147I AGGCCT 2 cut(s) 287, 618
Eco47I GGWCC 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 754, 968
EcoT14I CCWWGG 2 cut(s) 124, 282
ErhI CCWWGG 2 cut(s) 124, 282
FaeI CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 8 cut(s) 124, 186, 313, 388, 459, 600, 736, 898
FauI CCCGC 1 cut(s) 403
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 6 cut(s) 151, 176, 195, 580, 741, 912
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 6 cut(s) 151, 176, 195, 580, 741, 912
FspBI CTAG 2 cut(s) 87, 342
GluI GCNGC 6 cut(s) 151, 176, 195, 580, 741, 912
GsuI CTGGAG 1 cut(s) 991
HaeIII GGCC 4 cut(s) 287, 360, 495, 618
HapII CCGG 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
HinfI GANTC 4 cut(s) 130, 294, 686, 761
HpaII CCGG 1 cut(s) 261
HphI GGTGA 4 cut(s) 275, 338, 386, 569
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 3 cut(s) 138, 307, 835
Hpy188III TCNNGA 2 cut(s) 857, 997
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 4 cut(s) 97, 100, 386, 503
HpyAV CCTTC 3 cut(s) 189, 316, 975
HpyCH4III ACNGT 2 cut(s) 533, 632
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 6 cut(s) 707, 808, 845, 871, 911, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 725, 740, 827
HpyF3I CTNAG 3 cut(s) 624, 744, 879
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 6 cut(s) 6, 112, 307, 411, 434, 794
LmnI GCTCC 3 cut(s) 147, 449, 1009
Lsp1109I GCAGC 5 cut(s) 162, 162, 206, 591, 923
LweI GCATC 2 cut(s) 913, 1006
MaeI CTAG 2 cut(s) 87, 342
MaeII ACGT 1 cut(s) 95
MalI GATC 6 cut(s) 8, 114, 309, 413, 436, 796
MboI GATC 6 cut(s) 6, 112, 307, 411, 434, 794
MflI RGATCY 2 cut(s) 434, 794
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 3 cut(s) 236, 267, 935
MlyI GAGTC 2 cut(s) 303, 695
MmeI TCCRAC 3 cut(s) 427, 772, 858
MroXI GAANNNNTTC 1 cut(s) 31
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 2 cut(s) 756, 970
MvaI CCWGG 2 cut(s) 756, 970
MwoI GCNNNNNNNGC 3 cut(s) 725, 740, 827
NcoI CCATGG 1 cut(s) 124
NdeII GATC 6 cut(s) 6, 112, 307, 411, 434, 794
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
NlaIV GGNNCC 5 cut(s) 210, 394, 544, 796, 829
NspI RCATGY 2 cut(s) 604, 740
PceI AGGCCT 2 cut(s) 287, 618
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 130, 761
PflMI CCANNNNNTGG 1 cut(s) 647
PfoI TCCNGGA 1 cut(s) 968
PkrI GCNGC 6 cut(s) 152, 177, 196, 581, 742, 913
PleI GAGTC 2 cut(s) 302, 694
PpsI GAGTC 2 cut(s) 302, 694
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 905
Psp6I CCWGG 2 cut(s) 754, 968
PspGI CCWGG 2 cut(s) 754, 968
PspN4I GGNNCC 5 cut(s) 210, 394, 544, 796, 829
PspPI GGNCC 3 cut(s) 358, 392, 493
PstI CTGCAG 2 cut(s) 810, 873
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 434, 794
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 4 cut(s) 91, 629, 639, 979
RsaNI GTAC 4 cut(s) 90, 628, 638, 978
SatI GCNGC 6 cut(s) 151, 176, 195, 580, 741, 912
Sau3AI GATC 6 cut(s) 6, 112, 307, 411, 434, 794
Sau96I GGNCC 3 cut(s) 358, 392, 493
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 2 cut(s) 303, 695
ScrFI CCNGG 2 cut(s) 756, 970
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 2 cut(s) 913, 1006
SfcI CTRYAG 4 cut(s) 399, 806, 869, 946
SinI GGWCC 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 3 cut(s) 236, 267, 935
SseBI AGGCCT 2 cut(s) 287, 618
SsiI CCGC 4 cut(s) 10, 396, 577, 740
SspI AATATT 1 cut(s) 1049
SspMI CTAG 2 cut(s) 87, 342
StuI AGGCCT 2 cut(s) 287, 618
StyD4I CCNGG 2 cut(s) 754, 968
StyI CCWWGG 2 cut(s) 124, 282
TaaI ACNGT 2 cut(s) 533, 632
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 384, 849
TasI AATT 3 cut(s) 236, 267, 935
TatI WGTACW 1 cut(s) 627
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 2 cut(s) 130, 761
TscAI CASTG 2 cut(s) 19, 589
TseI GCWGC 5 cut(s) 150, 175, 194, 579, 911
TspDTI ATGAA 1 cut(s) 45
TspRI CASTG 2 cut(s) 19, 589
Van91I CCANNNNNTGG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 2 cut(s) 236, 267
XceI RCATGY 2 cut(s) 604, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 2 cut(s) 87, 342
ZraI GACGTC 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.