Rh2BG313600

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010272
Physical Location & Seq
Reverse (-)
36903534 .. 36910107
6574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG313600.1

Sequence Viewer

Length: 948 bp
ATGATGATCCGCAGTGCCATCGCCAGAAGACTTTCCTCAAAGCCAACCTCGTCTTCATCTTCTTATGCTTGGTTGACCGCCACCACTAGTACGACGTCGTTTGAGTTGGTTGATCAAAGCCTCCATGGGAATCATCAGAAGAGGAGGAGCAGCACGAGCTCATTACCTGTTGGGCTGCCTTCTTACATGAGAGCAGCCGTCTTCTGGGAACCAAACAAGCCTCTCTCCATTGAAGAATTCCACATCCCTCGCCCCAAATCCGGTGAAATTCTCATCAAAACCAAGGCCTGTGGAGTCTGCCATTCTGATCTCCATGTTTTGAAGGGTGAGCTTCCATTCGCTAGCCCTTGTGTCGTGGGCCACGAGATTACTGGGGAGGTTGTCGAACATGGACCCGCTACGGACAAAAAGATCACAGAAAGATTACCGATCGGATCTAATGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTTCTTCTGTTCAAAGGGCCACGACGATTTATGTGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGGAGCAGTGGAAAACCCGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGGATTGGCTATTTTACCGAAGTCATTGCCATATACAGAGTCTGCCATTTTAGGCTGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGCGGCTGAGGTTCGTCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGTGTTGGATCCAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGACATTATTGCAGTCGATATTCAAGACGACAAACTGCAGAAAGCTAAGATATTTGGTGCTACTCATGTTATAAATGCGGCTAAAGAAGATGCTGTTGAAAAATTATTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

315

Amino Acids

33.69

Weight (kDa)

7.58

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 87 - 222 3.7e-23 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 263 - 313 4.7e-12 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AatII GACGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 647
AccI GTMKAC 1 cut(s) 597
AccIII TCCGGA 1 cut(s) 576
AciI CCGC 5 cut(s) 10, 78, 396, 740, 911
AclWI GGATC 3 cut(s) 442, 789, 802
AcsI RAATTY 2 cut(s) 236, 267
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 91, 629, 639
AfiI CCNNNNNNNGG 3 cut(s) 204, 260, 647
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 5 cut(s) 233, 322, 489, 857, 932
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 6 cut(s) 159, 331, 452, 474, 801, 878
AluI AGCT 6 cut(s) 159, 331, 452, 474, 801, 878
Alw21I GWGCWC 1 cut(s) 161
AlwI GGATC 3 cut(s) 442, 789, 802
AlwNI CAGNNNCTG 1 cut(s) 689
Aor13HI TCCGGA 1 cut(s) 576
AoxI GGCC 4 cut(s) 285, 358, 493, 616
ApeKI GCWGC 3 cut(s) 150, 175, 194
ApoI RAATTY 2 cut(s) 236, 267
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 358, 392, 493
AsuHPI GGTGA 3 cut(s) 275, 338, 569
AsuNHI GCTAGC 1 cut(s) 341
AvaII GGWCC 1 cut(s) 392
BamHI GGATCC 1 cut(s) 794
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 161
BauI CACGAG 2 cut(s) 154, 362
BbsI GAAGAC 3 cut(s) 34, 45, 193
Bbv12I GWGCWC 1 cut(s) 161
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 3 cut(s) 162, 162, 206
BccI CCATC 2 cut(s) 26, 548
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 5 cut(s) 35, 655, 689, 824, 858
BciT130I CCWGG 1 cut(s) 756
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 2 cut(s) 87, 342
BfmI CTRYAG 2 cut(s) 806, 869
BisI GCNGC 5 cut(s) 151, 176, 195, 741, 912
BlsI GCNGC 5 cut(s) 152, 177, 196, 742, 913
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 358, 392, 493
BmiI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BmrFI CCNGG 1 cut(s) 756
BmrI ACTGGG 2 cut(s) 381, 792
BmsI GCATC 1 cut(s) 913
BmtI GCTAGC 1 cut(s) 345
BmuI ACTGGG 2 cut(s) 381, 792
BpiI GAAGAC 3 cut(s) 34, 45, 193
Bpu10I CCTNAGC 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 124, 282, 755
BsaWI WCCGGW 2 cut(s) 260, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 260, 647
Bse1I ACTGG 2 cut(s) 376, 787
Bse3DI GCAATG 1 cut(s) 671
BseAI TCCGGA 1 cut(s) 576
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 124, 282, 755
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 615, 735
BseNI ACTGG 2 cut(s) 376, 787
BseRI GAGGAG 2 cut(s) 157, 160
BseXI GCAGC 3 cut(s) 162, 162, 206
BsgI GTGCAG 1 cut(s) 726
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 287, 360, 495, 618
BshNI GGYRCC 1 cut(s) 827
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 2 cut(s) 261, 577
BslI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BsnI GGCC 4 cut(s) 287, 360, 495, 618
Bsp1286I GDGCHC 1 cut(s) 161
Bsp13I TCCGGA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 5 cut(s) 10, 78, 396, 740, 911
BspANI GGCC 4 cut(s) 287, 360, 495, 618
BspCNI CTCAG 2 cut(s) 616, 736
BspEI TCCGGA 1 cut(s) 576
BspLI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BspMAI CTGCAG 2 cut(s) 810, 873
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 442, 789, 802
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 376, 787
BssECI CCNNGG 3 cut(s) 124, 282, 755
BssMI GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
BssNAI GTATAC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 2 cut(s) 154, 362
BssT1I CCWWGG 2 cut(s) 124, 282
Bst1107I GTATAC 1 cut(s) 598
Bst2BI CACGAG 2 cut(s) 154, 362
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 2 cut(s) 533, 632
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 624, 744, 879
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 243
BstKTI GATC 7 cut(s) 9, 115, 310, 414, 432, 437, 797
BstMBI GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 4 cut(s) 156, 725, 740, 827
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 2 cut(s) 604, 740
BstSCI CCNGG 1 cut(s) 754
BstSFI CTRYAG 2 cut(s) 806, 869
BstV1I GCAGC 3 cut(s) 162, 162, 206
BstV2I GAAGAC 3 cut(s) 34, 45, 193
BstX2I RGATCY 2 cut(s) 434, 794
BstYI RGATCY 2 cut(s) 434, 794
BstZ17I GTATAC 1 cut(s) 598
BsuRI GGCC 4 cut(s) 287, 360, 495, 618
BtgI CCRYGG 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 4
BtsCI GGATG 1 cut(s) 243
BtsI GCAGTG 2 cut(s) 19, 589
BtsIMutI CAGTG 2 cut(s) 19, 589
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 689
Cfr13I GGNCC 3 cut(s) 358, 392, 493
Csp6I GTAC 3 cut(s) 90, 628, 638
CspCI CAANNNNNGTGG 2 cut(s) 271, 306
CviAII CATG 8 cut(s) 125, 187, 314, 389, 460, 601, 737, 899
CviQI GTAC 3 cut(s) 90, 628, 638
DdeI CTNAG 3 cut(s) 624, 744, 879
DpnI GATC 7 cut(s) 8, 114, 309, 413, 431, 436, 796
DpnII GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 124, 282
Eco147I AGGCCT 2 cut(s) 287, 618
Eco24I GRGCYC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 159
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 754
EcoT14I CCWWGG 2 cut(s) 124, 282
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 2 cut(s) 124, 282
FaeI CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 8 cut(s) 124, 186, 313, 388, 459, 600, 736, 898
FauI CCCGC 1 cut(s) 403
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 151, 176, 195, 741, 912
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 151, 176, 195, 741, 912
FspBI CTAG 2 cut(s) 87, 342
GluI GCNGC 5 cut(s) 151, 176, 195, 741, 912
HaeIII GGCC 4 cut(s) 287, 360, 495, 618
HapII CCGG 2 cut(s) 261, 577
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 4 cut(s) 130, 294, 686, 761
HpaII CCGG 2 cut(s) 261, 577
HphI GGTGA 3 cut(s) 275, 338, 569
Hpy166II GTNNAC 2 cut(s) 75, 598
Hpy188I TCNGA 4 cut(s) 138, 307, 434, 835
Hpy188III TCNNGA 2 cut(s) 577, 857
Hpy8I GTNNAC 2 cut(s) 75, 598
Hpy99I CGWCG 3 cut(s) 97, 100, 503
HpyAV CCTTC 2 cut(s) 189, 316
HpyCH4III ACNGT 2 cut(s) 533, 632
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 4 cut(s) 707, 808, 845, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 725, 740, 827
HpyF3I CTNAG 3 cut(s) 624, 744, 879
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
Kpn2I TCCGGA 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
LmnI GCTCC 3 cut(s) 147, 449, 579
Lsp1109I GCAGC 3 cut(s) 162, 162, 206
LweI GCATC 1 cut(s) 913
MaeI CTAG 2 cut(s) 87, 342
MaeII ACGT 1 cut(s) 95
MalI GATC 7 cut(s) 8, 114, 309, 413, 431, 436, 796
MboI GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
MboII GAAGA 8 cut(s) 39, 45, 51, 151, 193, 245, 472, 932
MflI RGATCY 2 cut(s) 434, 794
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 3 cut(s) 236, 267, 935
MlyI GAGTC 2 cut(s) 303, 695
MmeI TCCRAC 3 cut(s) 427, 772, 858
MroI TCCGGA 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 31
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 2 cut(s) 261, 577
MspR9I CCNGG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 4 cut(s) 156, 725, 740, 827
NcoI CCATGG 1 cut(s) 124
NdeII GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
NlaIV GGNNCC 5 cut(s) 210, 394, 544, 796, 829
NspI RCATGY 2 cut(s) 604, 740
PceI AGGCCT 2 cut(s) 287, 618
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 130, 761
PflMI CCANNNNNTGG 1 cut(s) 647
PkrI GCNGC 5 cut(s) 152, 177, 196, 742, 913
Ple19I CGATCG 1 cut(s) 432
PleI GAGTC 2 cut(s) 302, 694
PpsI GAGTC 2 cut(s) 302, 694
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 5 cut(s) 210, 394, 544, 796, 829
PspPI GGNCC 3 cut(s) 358, 392, 493
PstI CTGCAG 2 cut(s) 810, 873
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 434, 794
PvuI CGATCG 1 cut(s) 432
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 3 cut(s) 91, 629, 639
RsaNI GTAC 3 cut(s) 90, 628, 638
SacI GAGCTC 1 cut(s) 161
SatI GCNGC 5 cut(s) 151, 176, 195, 741, 912
Sau3AI GATC 7 cut(s) 6, 112, 307, 411, 429, 434, 794
Sau96I GGNCC 3 cut(s) 358, 392, 493
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 2 cut(s) 303, 695
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 1 cut(s) 161
SfaNI GCATC 1 cut(s) 913
SfcI CTRYAG 2 cut(s) 806, 869
SinI GGWCC 1 cut(s) 392
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 3 cut(s) 236, 267, 935
SseBI AGGCCT 2 cut(s) 287, 618
SsiI CCGC 5 cut(s) 10, 78, 396, 740, 911
SspMI CTAG 2 cut(s) 87, 342
SstI GAGCTC 1 cut(s) 161
StuI AGGCCT 2 cut(s) 287, 618
StyD4I CCNGG 1 cut(s) 754
StyI CCWWGG 2 cut(s) 124, 282
TaaI ACNGT 2 cut(s) 533, 632
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 384, 849
TasI AATT 3 cut(s) 236, 267, 935
TatI WGTACW 1 cut(s) 627
TauI GCSGC 2 cut(s) 743, 914
TfiI GAWTC 2 cut(s) 130, 761
TscAI CASTG 2 cut(s) 19, 589
TseI GCWGC 3 cut(s) 150, 175, 194
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 19, 589
Van91I CCANNNNNTGG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 2 cut(s) 236, 267
XceI RCATGY 2 cut(s) 604, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 2 cut(s) 87, 342
ZraI GACGTC 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.