Rroxscaffold_2G00120540

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010272
Physical Location & Seq
Forward (+)
52847247 .. 52854331
7085 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00120540.1

Sequence Viewer

Length: 1317 bp
ATGATGATGATGATCCGCAGTGCCATCGCCAGAAGACTTTCCTCAAAGCCAGCCTCGTCTTCTTCTTATTATGCTTGGTTGAGTACCACCACCACCACCACCACTAGTACGACGTCGTTTGAGTTGGTTGATCAAAGCCTCCATGGGAATCATCAGAAGAGGAGGAGCAGCTCATTGCCTGTTGGGCTGCCCTCTTACATGAGAGCAGCTGTCTTCTGGGAACCAAACAAACCTCTCTCCATTGAAGAATTCCACATCCCTCGCCCCAAATCCGGTGAAATTCTCATCAAAACCAAGGCCTGTGGAGTCTGCCATTCTGATCTCCATGTTTTGAAGGGTGAGCTTCCATTTGCTAGCCCTTGTGTCGTGGGCCACGAGATTACTGGCGAGGTTGTCGAACATGGACCCGCTACAGACAAAAAGATCACAGAAAGATTACCGATTGGATCTAATGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTTCTTCTGTTCAAAGGGCCACGACGATTTATGTGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGTAGCAGTGGAAAACCCGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGGATTGGCTATTTTGCCAAAGTCATTGCCATATACAGAGTCTGCCATTTTAGGCTGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGCGGCTGAGGTTCGTCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGTGTTGGATCCAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGACATTATTGCCGTCGATATTCAAGACGACAAACTGCAGAAAGCTAAGATATTTGGTGCTACTCATGTTATAAATGCGGCTAAAGAAGATGCTGTTGAAAAATTATTGGAAATAACTGGTGGGAAAGGTGTGGACGTTGCTGTGGAAGCCCTGGGAAAACCACAGACCTTTTTGCAATGCACACAAAGTGTGAGAGATGGAGGAAAAGCTGTCTTCATCGGGCTTACAGCAGCCGGAGCAATTGGGGAAGTAGATATTAACCGTCTTGTTCGTAGAAAGATAAAAATCATTGGATCATACGGAGGAAGGGCAAGGCAGGATCTTCCAAAGATAATTAGACTAGCAGAAAGTGGTATATTTGATCTCACCAGTGCTGTTTCAAGAAGATACAAATTCGAGGAGTCTGACACTGCATTCCAGGATCTCAACCAGGGAAAAATTGTTGGTCGGGCAGTGGTTGAGATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

438

Amino Acids

47.04

Weight (kDa)

8.53

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 91 - 226 7.7e-23 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 249 - 425 2.8e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 267 - 398 3.3e-33 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 300 - 435 2.8e-14 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 917
AatII GACGTC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 659
AccI GTMKAC 1 cut(s) 609
AciI CCGC 4 cut(s) 16, 408, 752, 923
AclWI GGATC 7 cut(s) 7, 454, 801, 814, 1147, 1173, 1275
AcsI RAATTY 3 cut(s) 248, 279, 1238
AcyI GRCGYC 1 cut(s) 113
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 4 cut(s) 85, 109, 641, 651
AfiI CCNNNNNNNGG 2 cut(s) 272, 659
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 6 cut(s) 245, 334, 501, 869, 944, 1227
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 4 cut(s) 766, 996, 1263, 1275
AluBI AGCT 8 cut(s) 171, 209, 343, 464, 486, 813, 890, 1055
AluI AGCT 8 cut(s) 171, 209, 343, 464, 486, 813, 890, 1055
AlwI GGATC 7 cut(s) 7, 454, 801, 814, 1147, 1173, 1275
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 4 cut(s) 297, 370, 505, 628
ApeKI GCWGC 4 cut(s) 168, 187, 206, 1076
ApoI RAATTY 3 cut(s) 248, 279, 1238
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 3 cut(s) 370, 404, 505
AsuHPI GGTGA 4 cut(s) 287, 350, 581, 1204
AsuNHI GCTAGC 1 cut(s) 353
AvaII GGWCC 1 cut(s) 404
BamHI GGATCC 1 cut(s) 806
BanI GGYRCC 1 cut(s) 839
BauI CACGAG 1 cut(s) 374
BbsI GAAGAC 4 cut(s) 40, 51, 205, 1051
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 4 cut(s) 174, 180, 218, 1088
BccI CCATC 3 cut(s) 32, 560, 1037
BceAI ACGGC 1 cut(s) 842
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 836, 870
BciT130I CCWGG 4 cut(s) 768, 998, 1265, 1277
BclI TGATCA 1 cut(s) 130
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 3 cut(s) 105, 354, 1187
BfmI CTRYAG 3 cut(s) 411, 818, 881
BglI GCCNNNNNGGC 1 cut(s) 184
BisI GCNGC 6 cut(s) 169, 188, 207, 753, 924, 1077
BlsI GCNGC 6 cut(s) 170, 189, 208, 754, 925, 1078
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 4 cut(s) 768, 998, 1265, 1277
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 370, 404, 505
BmiI GGNNCC 5 cut(s) 222, 406, 556, 808, 841
BmrFI CCNGG 4 cut(s) 768, 998, 1265, 1277
BmrI ACTGGG 1 cut(s) 804
BmsI GCATC 1 cut(s) 925
BmtI GCTAGC 1 cut(s) 357
BmuI ACTGGG 1 cut(s) 804
BpiI GAAGAC 4 cut(s) 40, 51, 205, 1051
Bpu10I CCTNAGC 1 cut(s) 756
BsaBI GATNNNNATC 2 cut(s) 11, 1130
BsaHI GRCGYC 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 142, 294, 767, 996, 997, 1276
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 659
Bse1I ACTGG 4 cut(s) 388, 799, 967, 1215
Bse3DI GCAATG 3 cut(s) 173, 683, 1028
Bse8I GATNNNNATC 2 cut(s) 11, 1130
BseBI CCWGG 4 cut(s) 768, 998, 1265, 1277
BseDI CCNNGG 6 cut(s) 142, 294, 767, 996, 997, 1276
BseGI GGATG 1 cut(s) 255
BseJI GATNNNNATC 2 cut(s) 11, 1130
BseLI CCNNNNNNNGG 2 cut(s) 272, 659
BseMI GCAATG 3 cut(s) 173, 683, 1028
BseMII CTCAG 2 cut(s) 627, 747
BseNI ACTGG 4 cut(s) 388, 799, 967, 1215
BseRI GAGGAG 3 cut(s) 175, 178, 1259
BseXI GCAGC 4 cut(s) 174, 180, 218, 1088
BsgI GTGCAG 1 cut(s) 738
BshFI GGCC 4 cut(s) 299, 372, 507, 630
BshNI GGYRCC 1 cut(s) 839
BsiSI CCGG 2 cut(s) 273, 1080
BslI CCNNNNNNNGG 2 cut(s) 272, 659
BsmI GAATGC 1 cut(s) 1259
BsnI GGCC 4 cut(s) 299, 372, 507, 630
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 4 cut(s) 16, 408, 752, 923
BspANI GGCC 4 cut(s) 299, 372, 507, 630
BspCNI CTCAG 2 cut(s) 628, 748
BspLI GGNNCC 5 cut(s) 222, 406, 556, 808, 841
BspMAI CTGCAG 2 cut(s) 822, 885
BspOI GCTAGC 1 cut(s) 357
BspPI GGATC 7 cut(s) 7, 454, 801, 814, 1147, 1173, 1275
BspT107I GGYRCC 1 cut(s) 839
BsrDI GCAATG 3 cut(s) 173, 683, 1028
BsrI ACTGG 4 cut(s) 388, 799, 967, 1215
BssECI CCNNGG 6 cut(s) 142, 294, 767, 996, 997, 1276
BssNAI GTATAC 1 cut(s) 610
BssNI GRCGYC 1 cut(s) 113
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 2 cut(s) 142, 294
Bst1107I GTATAC 1 cut(s) 610
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 4 cut(s) 768, 998, 1265, 1277
Bst4CI ACNGT 3 cut(s) 545, 644, 1109
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 839
BstC8I GCNNGC 2 cut(s) 51, 355
BstDEI CTNAG 3 cut(s) 636, 756, 891
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 255
BstMWI GCNNNNNNNGC 6 cut(s) 184, 737, 752, 839, 992, 1082
BstNI CCWGG 4 cut(s) 768, 998, 1265, 1277
BstNSI RCATGY 2 cut(s) 616, 752
BstSCI CCNGG 4 cut(s) 766, 996, 1263, 1275
BstSFI CTRYAG 3 cut(s) 411, 818, 881
BstV1I GCAGC 4 cut(s) 174, 180, 218, 1088
BstV2I GAAGAC 4 cut(s) 40, 51, 205, 1051
BstX2I RGATCY 4 cut(s) 446, 806, 1165, 1267
BstYI RGATCY 4 cut(s) 446, 806, 1165, 1267
BstZ17I GTATAC 1 cut(s) 610
BsuRI GGCC 4 cut(s) 299, 372, 507, 630
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 10
BtsCI GGATG 1 cut(s) 255
BtsI GCAGTG 4 cut(s) 25, 601, 1254, 1305
BtsIMutI CAGTG 5 cut(s) 25, 601, 1222, 1254, 1305
Cac8I GCNNGC 2 cut(s) 51, 355
CaiI CAGNNNCTG 1 cut(s) 701
Cfr13I GGNCC 3 cut(s) 370, 404, 505
Csp6I GTAC 4 cut(s) 84, 108, 640, 650
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 8 cut(s) 143, 199, 326, 401, 472, 613, 749, 911
CviQI GTAC 4 cut(s) 84, 108, 640, 650
DdeI CTNAG 3 cut(s) 636, 756, 891
DraIII CACNNNGTG 1 cut(s) 1034
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 142, 294
Eco147I AGGCCT 2 cut(s) 299, 630
Eco47I GGWCC 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 4 cut(s) 766, 996, 1263, 1275
EcoT14I CCWWGG 2 cut(s) 142, 294
ErhI CCWWGG 2 cut(s) 142, 294
FaeI CATG 8 cut(s) 146, 202, 329, 404, 475, 616, 752, 914
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FatI CATG 8 cut(s) 142, 198, 325, 400, 471, 612, 748, 910
FauI CCCGC 1 cut(s) 415
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 6 cut(s) 169, 188, 207, 753, 924, 1077
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 6 cut(s) 169, 188, 207, 753, 924, 1077
FspBI CTAG 3 cut(s) 105, 354, 1187
GluI GCNGC 6 cut(s) 169, 188, 207, 753, 924, 1077
HaeIII GGCC 4 cut(s) 299, 372, 507, 630
HapII CCGG 2 cut(s) 273, 1080
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 8 cut(s) 146, 202, 329, 404, 475, 616, 752, 914
HinfI GANTC 5 cut(s) 148, 306, 698, 773, 1247
HpaII CCGG 2 cut(s) 273, 1080
HphI GGTGA 4 cut(s) 287, 350, 581, 1204
Hpy166II GTNNAC 2 cut(s) 610, 979
Hpy188I TCNGA 4 cut(s) 156, 319, 847, 1252
Hpy188III TCNNGA 2 cut(s) 869, 1227
Hpy8I GTNNAC 2 cut(s) 610, 979
Hpy99I CGWCG 4 cut(s) 115, 118, 515, 863
HpyAV CCTTC 2 cut(s) 328, 1146
HpyCH4III ACNGT 3 cut(s) 545, 644, 1109
HpyCH4IV ACGT 2 cut(s) 113, 981
HpyCH4V TGCA 6 cut(s) 719, 820, 883, 1021, 1026, 1259
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 737, 752, 839, 992, 1082
HpyF3I CTNAG 3 cut(s) 636, 756, 891
HpySE526I ACGT 2 cut(s) 113, 981
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 8 cut(s) 146, 202, 329, 404, 475, 616, 752, 914
Ksp22I TGATCA 1 cut(s) 130
LmnI GCTCC 3 cut(s) 165, 461, 1082
Lsp1109I GCAGC 4 cut(s) 174, 180, 218, 1088
LweI GCATC 1 cut(s) 925
MaeI CTAG 3 cut(s) 105, 354, 1187
MaeII ACGT 2 cut(s) 113, 981
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 4 cut(s) 446, 806, 1165, 1267
MluCI AATT 7 cut(s) 248, 279, 947, 1086, 1179, 1238, 1284
MlyI GAGTC 3 cut(s) 315, 707, 1256
MmeI TCCRAC 3 cut(s) 439, 784, 870
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 1 cut(s) 1104
MspA1I CMGCKG 2 cut(s) 209, 813
MspI CCGG 2 cut(s) 273, 1080
MspR9I CCNGG 4 cut(s) 768, 998, 1265, 1277
MunI CAATTG 1 cut(s) 1086
Mva1269I GAATGC 1 cut(s) 1259
MvaI CCWGG 4 cut(s) 768, 998, 1265, 1277
MwoI GCNNNNNNNGC 6 cut(s) 184, 737, 752, 839, 992, 1082
NcoI CCATGG 1 cut(s) 142
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 8 cut(s) 146, 202, 329, 404, 475, 616, 752, 914
NlaIV GGNNCC 5 cut(s) 222, 406, 556, 808, 841
NspI RCATGY 2 cut(s) 616, 752
PasI CCCWGGG 1 cut(s) 997
PceI AGGCCT 2 cut(s) 299, 630
PciI ACATGT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 1259
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 2 cut(s) 148, 773
PflMI CCANNNNNTGG 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 1263
PkrI GCNGC 6 cut(s) 170, 189, 208, 754, 925, 1078
PleI GAGTC 3 cut(s) 314, 706, 1255
PpsI GAGTC 3 cut(s) 314, 706, 1255
PscI ACATGT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 917
Psp6I CCWGG 4 cut(s) 766, 996, 1263, 1275
PspGI CCWGG 4 cut(s) 766, 996, 1263, 1275
PspN4I GGNNCC 5 cut(s) 222, 406, 556, 808, 841
PspPI GGNCC 3 cut(s) 370, 404, 505
PstI CTGCAG 2 cut(s) 822, 885
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 4 cut(s) 446, 806, 1165, 1267
PvuII CAGCTG 2 cut(s) 209, 813
RsaI GTAC 4 cut(s) 85, 109, 641, 651
RsaNI GTAC 4 cut(s) 84, 108, 640, 650
SaqAI TTAA 1 cut(s) 1104
SatI GCNGC 6 cut(s) 169, 188, 207, 753, 924, 1077
Sau96I GGNCC 3 cut(s) 370, 404, 505
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 3 cut(s) 315, 707, 1256
ScrFI CCNGG 4 cut(s) 768, 998, 1265, 1277
SfaNI GCATC 1 cut(s) 925
SfcI CTRYAG 3 cut(s) 411, 818, 881
SinI GGWCC 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 7 cut(s) 248, 279, 947, 1086, 1179, 1238, 1284
SseBI AGGCCT 2 cut(s) 299, 630
SsiI CCGC 4 cut(s) 16, 408, 752, 923
SspMI CTAG 3 cut(s) 105, 354, 1187
StuI AGGCCT 2 cut(s) 299, 630
StyD4I CCNGG 4 cut(s) 766, 996, 1263, 1275
StyI CCWWGG 2 cut(s) 142, 294
TaaI ACNGT 3 cut(s) 545, 644, 1109
TaiI ACGT 2 cut(s) 116, 984
TaqI TCGA 3 cut(s) 396, 861, 1242
TasI AATT 7 cut(s) 248, 279, 947, 1086, 1179, 1238, 1284
TatI WGTACW 1 cut(s) 639
TauI GCSGC 2 cut(s) 755, 926
TfiI GAWTC 2 cut(s) 148, 773
Tru1I TTAA 1 cut(s) 1104
Tru9I TTAA 1 cut(s) 1104
TscAI CASTG 5 cut(s) 25, 601, 1222, 1261, 1305
TseI GCWGC 4 cut(s) 168, 187, 206, 1076
TspDTI ATGAA 1 cut(s) 1051
TspGWI ACGGA 2 cut(s) 578, 1161
TspRI CASTG 5 cut(s) 25, 601, 1222, 1261, 1305
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 3 cut(s) 248, 279, 1238
XceI RCATGY 2 cut(s) 616, 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 3 cut(s) 105, 354, 1187
ZraI GACGTC 1 cut(s) 114
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.