Rw2G024500

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010272
Physical Location & Seq
Reverse (-)
36159724 .. 36165400
5677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024500.1

Sequence Viewer

Length: 1305 bp
ATGATGATCCGCAGTGCCATCGCCAGAAGACTTTCCTCAAAGCCAACCTCGTCTTCATCTTCTTATGCTTGGTTGACCGCCACCACTAGTACGACGTCGTTTGAGTTGGTTGATCAAAGCCTCCATGGGAATCATCAGAAGAGGAGGAGCAGCACGACCTCATTACCTGTTGGGCTGCCTTCTTACATGAGAGCAGCCGTCTTCTGGGAACCAAACAAGCCTCTCTCCATTGAAGAATTCCACATCCCTCGCCCCAAATCCGGTGAAATTCTCATCAAAACCAAGGCCTGCGGAGTCTGCCATTCTGATCTCCATGTTTTGAAGGGTGAGCTTCCATTCGCTAGCCCTTGTGTCGTGGGCCACGAGATTACTGGTGAGGTCGTCGAACATGGACCCGCTACAGACAAAAAGATCACAGAAAGATTACCGATCGGATCTAATGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTTCTTCTGTTCAAAGGGCCACGACGATTTATGTGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGGAGCAGTGGAAAACCCGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGGATTGGCTATTTTACCGAAGTCATTGCCATATACAGAGTCTGCCATTTTAGGCTGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGGGGCTGAGGTTCGTCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGTGTTGGATCCAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGACATTATTGCAGTCGATATTCAAGACGACAAACTGCAGAAAGCTAAGATATTTGGTGCTACTCATGTTATAAATGCGGCTAAAGAAGATGCTGTTGAAAAATTATTGGAAATAACTGGTGGGAAAGGTGTGGACGTTGCTGTGGAAGCCCTGGGAAAACCACAGACCTTTTTGCAATGCACGCAAAGTGTGAGAGATGGAGGAAAAGCTGTCTTCATCGGGCTTACAGCAGCCGGAGCAATTGGAGAAGTAGATATTAACCGTCTTGTTCGTAGAAAGATAAAAATAATTGGATCATACGGAGGAAGGGCAAGGCAGGATCTTCCAAAGATAGTTAGACTAGCAGAAAGTGGTATATTTGATCTCACCAGTGCTGTTTCAAGAAAATACAAATTCGAGGAGTCTGACACTGCATTCCAGGATCTCAACCAGGGAAAAATTGTTGGTCGGGCAGTGGTTGAGATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

434

Amino Acids

46.45

Weight (kDa)

8.53

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 87 - 222 7.5e-23 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 248 - 421 7.5e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 263 - 394 4.8e-33 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 296 - 431 3.3e-14 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AatII GACGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 647
AccI GTMKAC 1 cut(s) 597
AccIII TCCGGA 1 cut(s) 576
AciI CCGC 5 cut(s) 10, 78, 291, 396, 911
AclWI GGATC 6 cut(s) 442, 789, 802, 1135, 1161, 1263
AcsI RAATTY 3 cut(s) 236, 267, 1226
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 91, 629, 639
AfiI CCNNNNNNNGG 3 cut(s) 204, 260, 647
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 6 cut(s) 233, 322, 489, 857, 932, 1215
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 4 cut(s) 754, 984, 1251, 1263
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 6 cut(s) 331, 452, 474, 801, 878, 1043
AluI AGCT 6 cut(s) 331, 452, 474, 801, 878, 1043
AlwI GGATC 6 cut(s) 442, 789, 802, 1135, 1161, 1263
AlwNI CAGNNNCTG 1 cut(s) 689
Aor13HI TCCGGA 1 cut(s) 576
AoxI GGCC 4 cut(s) 285, 358, 493, 616
ApeKI GCWGC 4 cut(s) 150, 175, 194, 1064
ApoI RAATTY 3 cut(s) 236, 267, 1226
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 358, 392, 493
AsuHPI GGTGA 5 cut(s) 275, 338, 386, 569, 1192
AsuNHI GCTAGC 1 cut(s) 341
AvaII GGWCC 1 cut(s) 392
BamHI GGATCC 1 cut(s) 794
BanI GGYRCC 1 cut(s) 827
BauI CACGAG 1 cut(s) 362
BbsI GAAGAC 4 cut(s) 34, 45, 193, 1039
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 4 cut(s) 162, 162, 206, 1076
BccI CCATC 3 cut(s) 26, 548, 1025
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 5 cut(s) 35, 655, 689, 824, 858
BciT130I CCWGG 4 cut(s) 756, 986, 1253, 1265
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 3 cut(s) 87, 342, 1175
BfmI CTRYAG 3 cut(s) 399, 806, 869
BisI GCNGC 5 cut(s) 151, 176, 195, 912, 1065
BlsI GCNGC 5 cut(s) 152, 177, 196, 913, 1066
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 4 cut(s) 756, 986, 1253, 1265
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 358, 392, 493
BmiI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BmrFI CCNGG 4 cut(s) 756, 986, 1253, 1265
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 1 cut(s) 913
BmtI GCTAGC 1 cut(s) 345
BmuI ACTGGG 1 cut(s) 792
BpiI GAAGAC 4 cut(s) 34, 45, 193, 1039
Bpu10I CCTNAGC 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 6 cut(s) 124, 282, 755, 984, 985, 1264
BsaWI WCCGGW 2 cut(s) 260, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 260, 647
Bse1I ACTGG 4 cut(s) 376, 787, 955, 1203
Bse3DI GCAATG 2 cut(s) 671, 1016
BseAI TCCGGA 1 cut(s) 576
BseBI CCWGG 4 cut(s) 756, 986, 1253, 1265
BseDI CCNNGG 6 cut(s) 124, 282, 755, 984, 985, 1264
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BseMI GCAATG 2 cut(s) 671, 1016
BseMII CTCAG 2 cut(s) 615, 735
BseNI ACTGG 4 cut(s) 376, 787, 955, 1203
BseRI GAGGAG 3 cut(s) 157, 160, 1247
BseXI GCAGC 4 cut(s) 162, 162, 206, 1076
BsgI GTGCAG 1 cut(s) 726
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 287, 360, 495, 618
BshNI GGYRCC 1 cut(s) 827
BsiEI CGRYCG 1 cut(s) 432
BsiSI CCGG 3 cut(s) 261, 577, 1068
BslI CCNNNNNNNGG 3 cut(s) 204, 260, 647
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 4 cut(s) 287, 360, 495, 618
Bsp13I TCCGGA 1 cut(s) 576
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 5 cut(s) 10, 78, 291, 396, 911
BspANI GGCC 4 cut(s) 287, 360, 495, 618
BspCNI CTCAG 2 cut(s) 616, 736
BspEI TCCGGA 1 cut(s) 576
BspLI GGNNCC 5 cut(s) 210, 394, 544, 796, 829
BspMAI CTGCAG 2 cut(s) 810, 873
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 6 cut(s) 442, 789, 802, 1135, 1161, 1263
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 2 cut(s) 671, 1016
BsrI ACTGG 4 cut(s) 376, 787, 955, 1203
BssECI CCNNGG 6 cut(s) 124, 282, 755, 984, 985, 1264
BssNAI GTATAC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 2 cut(s) 124, 282
Bst1107I GTATAC 1 cut(s) 598
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 4 cut(s) 756, 986, 1253, 1265
Bst4CI ACNGT 3 cut(s) 533, 632, 1097
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 289, 343, 1016
BstDEI CTNAG 3 cut(s) 624, 744, 879
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 243
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 7 cut(s) 297, 725, 740, 827, 980, 1015, 1070
BstNI CCWGG 4 cut(s) 756, 986, 1253, 1265
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 4 cut(s) 754, 984, 1251, 1263
BstSFI CTRYAG 3 cut(s) 399, 806, 869
BstV1I GCAGC 4 cut(s) 162, 162, 206, 1076
BstV2I GAAGAC 4 cut(s) 34, 45, 193, 1039
BstX2I RGATCY 4 cut(s) 434, 794, 1153, 1255
BstYI RGATCY 4 cut(s) 434, 794, 1153, 1255
BstZ17I GTATAC 1 cut(s) 598
BsuRI GGCC 4 cut(s) 287, 360, 495, 618
BtgI CCRYGG 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 4
BtsCI GGATG 1 cut(s) 243
BtsI GCAGTG 4 cut(s) 19, 589, 1242, 1293
BtsIMutI CAGTG 5 cut(s) 19, 589, 1210, 1242, 1293
Cac8I GCNNGC 3 cut(s) 289, 343, 1016
CaiI CAGNNNCTG 1 cut(s) 689
Cfr13I GGNCC 3 cut(s) 358, 392, 493
Csp6I GTAC 3 cut(s) 90, 628, 638
CviAII CATG 8 cut(s) 125, 187, 314, 389, 460, 601, 737, 899
CviQI GTAC 3 cut(s) 90, 628, 638
DdeI CTNAG 3 cut(s) 624, 744, 879
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 2 cut(s) 124, 282
Eco147I AGGCCT 2 cut(s) 287, 618
Eco47I GGWCC 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 4 cut(s) 754, 984, 1251, 1263
EcoT14I CCWWGG 2 cut(s) 124, 282
ErhI CCWWGG 2 cut(s) 124, 282
FaeI CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 8 cut(s) 124, 186, 313, 388, 459, 600, 736, 898
FauI CCCGC 1 cut(s) 403
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 151, 176, 195, 912, 1065
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 151, 176, 195, 912, 1065
FspBI CTAG 3 cut(s) 87, 342, 1175
GluI GCNGC 5 cut(s) 151, 176, 195, 912, 1065
HaeIII GGCC 4 cut(s) 287, 360, 495, 618
HapII CCGG 3 cut(s) 261, 577, 1068
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 5 cut(s) 130, 294, 686, 761, 1235
HpaII CCGG 3 cut(s) 261, 577, 1068
HphI GGTGA 5 cut(s) 275, 338, 386, 569, 1192
Hpy166II GTNNAC 3 cut(s) 75, 598, 967
Hpy188I TCNGA 5 cut(s) 138, 307, 434, 835, 1240
Hpy188III TCNNGA 3 cut(s) 577, 857, 1215
Hpy8I GTNNAC 3 cut(s) 75, 598, 967
Hpy99I CGWCG 4 cut(s) 97, 100, 386, 503
HpyAV CCTTC 3 cut(s) 189, 316, 1134
HpyCH4III ACNGT 3 cut(s) 533, 632, 1097
HpyCH4IV ACGT 2 cut(s) 95, 969
HpyCH4V TGCA 7 cut(s) 707, 808, 845, 871, 1009, 1014, 1247
HpyF10VI GCNNNNNNNGC 7 cut(s) 297, 725, 740, 827, 980, 1015, 1070
HpyF3I CTNAG 3 cut(s) 624, 744, 879
HpySE526I ACGT 2 cut(s) 95, 969
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
Kpn2I TCCGGA 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 112
LmnI GCTCC 4 cut(s) 147, 449, 579, 1070
Lsp1109I GCAGC 4 cut(s) 162, 162, 206, 1076
LweI GCATC 1 cut(s) 913
MaeI CTAG 3 cut(s) 87, 342, 1175
MaeII ACGT 2 cut(s) 95, 969
MfeI CAATTG 1 cut(s) 1074
MflI RGATCY 4 cut(s) 434, 794, 1153, 1255
MluCI AATT 7 cut(s) 236, 267, 935, 1074, 1122, 1226, 1272
MlyI GAGTC 3 cut(s) 303, 695, 1244
MmeI TCCRAC 3 cut(s) 427, 772, 858
MroI TCCGGA 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 31
MseI TTAA 1 cut(s) 1092
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 3 cut(s) 261, 577, 1068
MspR9I CCNGG 4 cut(s) 756, 986, 1253, 1265
MunI CAATTG 1 cut(s) 1074
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 4 cut(s) 756, 986, 1253, 1265
MwoI GCNNNNNNNGC 7 cut(s) 297, 725, 740, 827, 980, 1015, 1070
NcoI CCATGG 1 cut(s) 124
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 8 cut(s) 128, 190, 317, 392, 463, 604, 740, 902
NlaIV GGNNCC 5 cut(s) 210, 394, 544, 796, 829
NspI RCATGY 1 cut(s) 604
PasI CCCWGGG 1 cut(s) 985
PceI AGGCCT 2 cut(s) 287, 618
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 1247
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 130, 761
PflMI CCANNNNNTGG 1 cut(s) 647
PfoI TCCNGGA 1 cut(s) 1251
PkrI GCNGC 5 cut(s) 152, 177, 196, 913, 1066
Ple19I CGATCG 1 cut(s) 432
PleI GAGTC 3 cut(s) 302, 694, 1243
PpsI GAGTC 3 cut(s) 302, 694, 1243
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 905
Psp6I CCWGG 4 cut(s) 754, 984, 1251, 1263
PspGI CCWGG 4 cut(s) 754, 984, 1251, 1263
PspN4I GGNNCC 5 cut(s) 210, 394, 544, 796, 829
PspPI GGNCC 3 cut(s) 358, 392, 493
PstI CTGCAG 2 cut(s) 810, 873
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 4 cut(s) 434, 794, 1153, 1255
PvuI CGATCG 1 cut(s) 432
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 3 cut(s) 91, 629, 639
RsaNI GTAC 3 cut(s) 90, 628, 638
SaqAI TTAA 1 cut(s) 1092
SatI GCNGC 5 cut(s) 151, 176, 195, 912, 1065
Sau96I GGNCC 3 cut(s) 358, 392, 493
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 3 cut(s) 303, 695, 1244
ScrFI CCNGG 4 cut(s) 756, 986, 1253, 1265
SfaNI GCATC 1 cut(s) 913
SfcI CTRYAG 3 cut(s) 399, 806, 869
SinI GGWCC 1 cut(s) 392
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 7 cut(s) 236, 267, 935, 1074, 1122, 1226, 1272
SseBI AGGCCT 2 cut(s) 287, 618
SsiI CCGC 5 cut(s) 10, 78, 291, 396, 911
SspMI CTAG 3 cut(s) 87, 342, 1175
StuI AGGCCT 2 cut(s) 287, 618
StyD4I CCNGG 4 cut(s) 754, 984, 1251, 1263
StyI CCWWGG 2 cut(s) 124, 282
TaaI ACNGT 3 cut(s) 533, 632, 1097
TaiI ACGT 2 cut(s) 98, 972
TaqI TCGA 3 cut(s) 384, 849, 1230
TasI AATT 7 cut(s) 236, 267, 935, 1074, 1122, 1226, 1272
TatI WGTACW 1 cut(s) 627
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 2 cut(s) 130, 761
Tru1I TTAA 1 cut(s) 1092
Tru9I TTAA 1 cut(s) 1092
TscAI CASTG 5 cut(s) 19, 589, 1210, 1249, 1293
TseI GCWGC 4 cut(s) 150, 175, 194, 1064
TspDTI ATGAA 2 cut(s) 45, 1039
TspGWI ACGGA 1 cut(s) 1149
TspRI CASTG 5 cut(s) 19, 589, 1210, 1249, 1293
Van91I CCANNNNNTGG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 3 cut(s) 236, 267, 1226
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 3 cut(s) 87, 342, 1175
ZraI GACGTC 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.