FvH4_1g25640

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005060
Physical Location & Seq
Reverse (-)
17483641 .. 17492729
9089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25640.t5

Sequence Viewer

Length: 1242 bp
ATGTGGACGACGGCGATTTTCAATTCGAAATCGGCGTTGTTACAGTTGGCGACACGGCGGCTGAGCACCGGGCCAGGGAAGAGAGTCGCCGCAGTTTGGGGGAACGGAGACTACGGGAGGCTCGGATTAGGGAGCTTGGACTCTCAGTGGAGCCCTACCCCTCTTCTCTGCTCTGCGTTTCGGGACCAAAGCCTCCAAGCCATTGCTTGCGGCGGAGCTCACACTCTGTTCTTGACAGACGGCGGCCGGGTTTTCGCCACCGGGCTTAATGATTTCGGACAGCTTGGGGTCTCCAGTGATGTGCCTTATTCCACTGAACCGGCGGAGGTATCGGGAATCGACAAGCAAGTTGTGCAAATTTCGACTGGATATTATCACTCTTGTGCTGTTACAGATGGAGAGTTATACATGTGGGGGAAGAACTCAAATGGGCAGCTTGGCCTCGGAAAGAGGGGAGCAAAAGCAGTTTCTATTCCAACAAAAGTTGAATGCTTAGCTGGAATGACCATTAGGATGGCAGCTTTGGGATCAGAGCACTCGGTGGCTGTCACTGATGGAGGTGAGTGTTTAAGTTGGGGAGAAGGGTCTGGAAGACTTGGTCATGCCCCTGAATCAGGCATTTTAGGAATTTTCAGAAGTACCAGTGAATATACACCAAGGCTTATCAAGAAACTTGAGGGGACCAAAGTTATAAAAGTTGCTGCTGGATTGCTGCATTCAGCATGTGTTGATGAAAATGGATCTGTATTCGTATTTGGTGAAAAAGCACTGGACAAAATGGGGAATGCCAAGGAAAAGAATGGAAATGCACCGTCTGTGATTGGCAAACTGCCATTTTCGGAAGAAGTCTCATGCGGTGGCTATCACACATGTGCCATAACAAGTGGTGGAGAACTCTACAGTTGGGGTTCAAATGAAAATGGTTGCCTTGGTATTGGTACCACAGATGTATTTCATCTGCCTGAAAGAGTTCAAGGTCCATTCTTGAAATCTGCTGTTGTCAAGGTATCTTGTGGTTGGAAGCATACAGCAGCAATTTCTGGACGCAATATCTTCACATGGGGTTGGGGAGGTTCGCACGGAACATTTTCTGAAGAAGGGCATTCTTCTGGAGGACAACTGGGTCACGGAACTGATGTTGACTACGTAAAACCTACAAGGGTTAAGTTCGGGGAGAATTTGAAAGCTTTACATATATCATGTGGGTTCAATCACACTGGTGCCATACTTGAATACGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

414

Amino Acids

43.42

Weight (kDa)

7.18

Isoelectric Point (pI)

31.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 31 - 411 1.1e-83 RCC1-like domain
RCC1 PF00415 33 - 78 9.6e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 67 - 94 1.2e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 82 - 130 1.9e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 117 - 150 1.5e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 137 - 188 1.7e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 192 - 248 1.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 288 - 314 5.8e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 302 - 351 9.2e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 373 - 414 2.7e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
AasI GACNNNNNNGTC 1 cut(s) 1122
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 2 cut(s) 938, 1220
AciI CCGC 7 cut(s) 58, 90, 210, 213, 243, 323, 855
AclWI GGATC 2 cut(s) 535, 748
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 3 cut(s) 357, 627, 1177
AcuI CTGAAG 1 cut(s) 1113
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 2 cut(s) 640, 940
AfiI CCNNNNNNNGG 3 cut(s) 75, 96, 614
AflIII ACRYGT 2 cut(s) 408, 869
AgsI TTSAA 8 cut(s) 22, 488, 912, 974, 988, 1183, 1210, 1232
AjnI CCWGG 1 cut(s) 73
AleI CACNNNNGTG 3 cut(s) 381, 870, 1218
AluBI AGCT 7 cut(s) 135, 218, 283, 436, 497, 521, 1187
AluI AGCT 7 cut(s) 135, 218, 283, 436, 497, 521, 1187
Alw21I GWGCWC 3 cut(s) 68, 220, 537
Alw26I GTCTC 3 cut(s) 102, 295, 853
AlwI GGATC 2 cut(s) 535, 748
AoxI GGCC 3 cut(s) 71, 244, 439
ApeKI GCWGC 5 cut(s) 433, 518, 701, 712, 1031
ApoI RAATTY 3 cut(s) 357, 627, 1177
Asp700I GAANNNNTTC 2 cut(s) 969, 1087
Asp718I GGTACC 1 cut(s) 938
AspS9I GGNCC 4 cut(s) 71, 184, 681, 977
AsuC2I CCSGG 3 cut(s) 70, 248, 262
AsuHPI GGTGA 2 cut(s) 572, 770
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 3 cut(s) 184, 681, 977
BanI GGYRCC 2 cut(s) 938, 1220
BanII GRGCYC 2 cut(s) 155, 220
BbsI GAAGAC 1 cut(s) 598
Bbv12I GWGCWC 3 cut(s) 68, 220, 537
BbvI GCAGC 5 cut(s) 445, 530, 688, 699, 1043
BccI CCATC 3 cut(s) 389, 508, 548
BceAI ACGGC 3 cut(s) 27, 71, 256
BciT130I CCWGG 1 cut(s) 75
BcnI CCSGG 3 cut(s) 70, 248, 262
BcoDI GTCTC 3 cut(s) 102, 295, 853
BfmI CTRYAG 1 cut(s) 898
BisI GCNGC 9 cut(s) 59, 90, 211, 244, 434, 519, 702, 713, 1032
BlpI GCTNAGC 2 cut(s) 62, 493
BlsI GCNGC 9 cut(s) 60, 91, 212, 245, 435, 520, 703, 714, 1033
Bme1390I CCNGG 4 cut(s) 70, 75, 248, 262
Bme18I GGWCC 3 cut(s) 184, 681, 977
BmgT120I GGNCC 4 cut(s) 71, 184, 681, 977
BmiI GGNNCC 5 cut(s) 152, 185, 682, 940, 1222
BmrFI CCNGG 4 cut(s) 70, 75, 248, 262
BmrI ACTGGG 1 cut(s) 1130
BmuI ACTGGG 1 cut(s) 1130
BpiI GAAGAC 1 cut(s) 598
BpmI CTGGAG 2 cut(s) 277, 1131
Bpu1102I GCTNAGC 2 cut(s) 62, 493
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 3 cut(s) 70, 248, 262
BsaAI YACGTR 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 5 cut(s) 74, 442, 656, 789, 928
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 96, 614
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 6 cut(s) 294, 370, 642, 774, 1125, 1222
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 74, 442, 656, 789, 928
BseGI GGATG 1 cut(s) 519
BseLI CCNNNNNNNGG 3 cut(s) 75, 96, 614
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 2 cut(s) 53, 158
BseNI ACTGG 6 cut(s) 294, 370, 642, 774, 1125, 1222
BseX3I CGGCCG 1 cut(s) 244
BseXI GCAGC 5 cut(s) 445, 530, 688, 699, 1043
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 3 cut(s) 73, 246, 441
BshNI GGYRCC 2 cut(s) 938, 1220
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 3 cut(s) 68, 220, 537
BsiSI CCGG 4 cut(s) 69, 247, 261, 320
BslFI GGGAC 2 cut(s) 197, 694
BslI CCNNNNNNNGG 3 cut(s) 75, 96, 614
BsmAI GTCTC 3 cut(s) 102, 295, 853
BsmFI GGGAC 2 cut(s) 197, 694
BsmI GAATGC 4 cut(s) 494, 715, 790, 1102
BsnI GGCC 3 cut(s) 73, 246, 441
Bso31I GGTCTC 1 cut(s) 295
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 4 cut(s) 68, 155, 220, 537
Bsp143I GATC 2 cut(s) 527, 740
Bsp1720I GCTNAGC 2 cut(s) 62, 493
BspACI CCGC 7 cut(s) 58, 90, 210, 213, 243, 323, 855
BspANI GGCC 3 cut(s) 73, 246, 441
BspCNI CTCAG 2 cut(s) 54, 157
BspLI GGNNCC 5 cut(s) 152, 185, 682, 940, 1222
BspPI GGATC 2 cut(s) 535, 748
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 2 cut(s) 938, 1220
BspTNI GGTCTC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 6 cut(s) 294, 370, 642, 774, 1125, 1222
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 5 cut(s) 74, 442, 656, 789, 928
BssMI GATC 2 cut(s) 527, 740
BssT1I CCWWGG 3 cut(s) 656, 789, 928
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 3 cut(s) 45, 813, 902
Bst6I CTCTTC 2 cut(s) 74, 168
BstAPI GCANNNNNTGC 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 1147
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 3 cut(s) 62, 144, 493
BstENI CCTNNNNNAGG 1 cut(s) 612
BstF5I GGATG 1 cut(s) 519
BstKTI GATC 2 cut(s) 530, 743
BstMAI GTCTC 3 cut(s) 102, 295, 853
BstMBI GATC 2 cut(s) 527, 740
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 3 cut(s) 412, 726, 873
BstSCI CCNGG 4 cut(s) 68, 73, 246, 260
BstSFI CTRYAG 1 cut(s) 898
BstSNI TACGTA 1 cut(s) 1147
BstV1I GCAGC 5 cut(s) 445, 530, 688, 699, 1043
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 740
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 1 cut(s) 740
BstZI CGGCCG 1 cut(s) 244
BsuRI GGCC 3 cut(s) 73, 246, 441
BtsCI GGATG 1 cut(s) 519
BtsIMutI CAGTG 7 cut(s) 152, 301, 312, 549, 649, 767, 1215
Cac8I GCNNGC 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 4 cut(s) 71, 184, 681, 977
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 2 cut(s) 639, 939
CviAII CATG 7 cut(s) 409, 602, 723, 852, 870, 1059, 1200
CviQI GTAC 2 cut(s) 639, 939
DdeI CTNAG 3 cut(s) 62, 144, 493
DpnI GATC 2 cut(s) 529, 742
DpnII GATC 2 cut(s) 527, 740
DraIII CACNNNGTG 1 cut(s) 541
DrdI GACNNNNNNGTC 1 cut(s) 1122
DseDI GACNNNNNNGTC 1 cut(s) 1122
EaeI YGGCCR 1 cut(s) 244
EagI CGGCCG 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 74, 168
EarI CTCTTC 2 cut(s) 74, 168
EciI GGCGGA 2 cut(s) 228, 338
Ecl136II GAGCTC 1 cut(s) 218
EclXI CGGCCG 1 cut(s) 244
Eco105I TACGTA 1 cut(s) 1147
Eco130I CCWWGG 3 cut(s) 656, 789, 928
Eco24I GRGCYC 2 cut(s) 155, 220
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 3 cut(s) 184, 681, 977
Eco52I CGGCCG 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 1113
EcoICRI GAGCTC 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 612
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 3 cut(s) 656, 789, 928
EcoT38I GRGCYC 2 cut(s) 155, 220
ErhI CCWWGG 3 cut(s) 656, 789, 928
FaeI CATG 7 cut(s) 412, 605, 726, 855, 873, 1062, 1203
FaqI GGGAC 2 cut(s) 197, 694
FatI CATG 7 cut(s) 408, 601, 722, 851, 869, 1058, 1199
Fnu4HI GCNGC 9 cut(s) 59, 90, 211, 244, 434, 519, 702, 713, 1032
FokI GGATG 1 cut(s) 526
FriOI GRGCYC 2 cut(s) 155, 220
Fsp4HI GCNGC 9 cut(s) 59, 90, 211, 244, 434, 519, 702, 713, 1032
GluI GCNGC 9 cut(s) 59, 90, 211, 244, 434, 519, 702, 713, 1032
GsuI CTGGAG 2 cut(s) 277, 1131
HaeIII GGCC 3 cut(s) 73, 246, 441
HapII CCGG 4 cut(s) 69, 247, 261, 320
HgaI GACGC 1 cut(s) 1053
Hin1II CATG 7 cut(s) 412, 605, 726, 855, 873, 1062, 1203
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HindIII AAGCTT 1 cut(s) 1185
HinfI GANTC 4 cut(s) 84, 140, 336, 611
HpaII CCGG 4 cut(s) 69, 247, 261, 320
HphI GGTGA 2 cut(s) 572, 770
Hpy166II GTNNAC 2 cut(s) 6, 1141
Hpy188I TCNGA 7 cut(s) 125, 278, 446, 532, 635, 841, 1093
Hpy188III TCNNGA 8 cut(s) 182, 232, 333, 588, 667, 985, 1041, 1110
Hpy8I GTNNAC 2 cut(s) 6, 1141
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 575, 1091
HpyCH4III ACNGT 3 cut(s) 45, 813, 902
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 3 cut(s) 355, 715, 809
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 3 cut(s) 62, 144, 493
HpySE526I ACGT 1 cut(s) 1146
Hsp92II CATG 7 cut(s) 412, 605, 726, 855, 873, 1062, 1203
KpnI GGTACC 1 cut(s) 942
Kzo9I GATC 2 cut(s) 527, 740
LmnI GCTCC 4 cut(s) 132, 150, 215, 455
Lsp1109I GCAGC 5 cut(s) 445, 530, 688, 699, 1043
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 4 cut(s) 39, 388, 547, 1124
MalI GATC 2 cut(s) 529, 742
MboI GATC 2 cut(s) 527, 740
MboII GAAGA 8 cut(s) 91, 155, 430, 603, 854, 1045, 1098, 1106
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 4 cut(s) 68, 155, 220, 537
MluCI AATT 5 cut(s) 22, 357, 627, 1035, 1177
MlyI GAGTC 2 cut(s) 93, 134
MmeI TCCRAC 2 cut(s) 500, 998
MroXI GAANNNNTTC 2 cut(s) 969, 1087
MseI TTAA 3 cut(s) 267, 569, 1164
MslI CAYNNNNRTG 4 cut(s) 381, 512, 870, 1218
MspI CCGG 4 cut(s) 69, 247, 261, 320
MspR9I CCNGG 4 cut(s) 70, 75, 248, 262
Mva1269I GAATGC 4 cut(s) 494, 715, 790, 1102
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 352
NciI CCSGG 3 cut(s) 70, 248, 262
NdeII GATC 2 cut(s) 527, 740
NlaIII CATG 7 cut(s) 412, 605, 726, 855, 873, 1062, 1203
NlaIV GGNNCC 5 cut(s) 152, 185, 682, 940, 1222
NmuCI GTSAC 2 cut(s) 547, 1124
NspI RCATGY 3 cut(s) 412, 726, 873
NspV TTCGAA 1 cut(s) 26
OliI CACNNNNGTG 3 cut(s) 381, 870, 1218
PciI ACATGT 2 cut(s) 408, 869
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PctI GAATGC 4 cut(s) 494, 715, 790, 1102
PdmI GAANNNNTTC 2 cut(s) 969, 1087
PfeI GAWTC 2 cut(s) 336, 611
PflFI GACNNNGTC 1 cut(s) 597
PkrI GCNGC 9 cut(s) 60, 91, 212, 245, 435, 520, 703, 714, 1033
PleI GAGTC 2 cut(s) 92, 134
PpsI GAGTC 2 cut(s) 92, 134
Ppu21I YACGTR 1 cut(s) 1147
PscI ACATGT 2 cut(s) 408, 869
PsiI TTATAA 1 cut(s) 692
Psp124BI GAGCTC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 5 cut(s) 152, 185, 682, 940, 1222
PspPI GGNCC 4 cut(s) 71, 184, 681, 977
PsrI GAACNNNNNNTAC 2 cut(s) 95, 127
PsuI RGATCY 1 cut(s) 740
PsyI GACNNNGTC 1 cut(s) 597
RsaI GTAC 2 cut(s) 640, 940
RsaNI GTAC 2 cut(s) 639, 939
RseI CAYNNNNRTG 4 cut(s) 381, 512, 870, 1218
SacI GAGCTC 1 cut(s) 220
SaqAI TTAA 3 cut(s) 267, 569, 1164
SatI GCNGC 9 cut(s) 59, 90, 211, 244, 434, 519, 702, 713, 1032
Sau3AI GATC 2 cut(s) 527, 740
Sau96I GGNCC 4 cut(s) 71, 184, 681, 977
SchI GAGTC 2 cut(s) 93, 134
ScrFI CCNGG 4 cut(s) 70, 75, 248, 262
SduI GDGCHC 4 cut(s) 68, 155, 220, 537
SfcI CTRYAG 1 cut(s) 898
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 3 cut(s) 184, 681, 977
SmiMI CAYNNNNRTG 4 cut(s) 381, 512, 870, 1218
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SnaBI TACGTA 1 cut(s) 1147
Sse9I AATT 5 cut(s) 22, 357, 627, 1035, 1177
SsiI CCGC 7 cut(s) 58, 90, 210, 213, 243, 323, 855
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 4 cut(s) 68, 73, 246, 260
StyI CCWWGG 3 cut(s) 656, 789, 928
TaaI ACNGT 3 cut(s) 45, 813, 902
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 3 cut(s) 26, 339, 362
TasI AATT 5 cut(s) 22, 357, 627, 1035, 1177
TauI GCSGC 4 cut(s) 61, 92, 213, 246
TfiI GAWTC 2 cut(s) 336, 611
Tru1I TTAA 3 cut(s) 267, 569, 1164
Tru9I TTAA 3 cut(s) 267, 569, 1164
TscAI CASTG 7 cut(s) 152, 301, 319, 556, 649, 774, 1222
TseFI GTSAC 2 cut(s) 547, 1124
TseI GCWGC 5 cut(s) 433, 518, 701, 712, 1031
Tsp45I GTSAC 2 cut(s) 547, 1124
TspDTI ATGAA 3 cut(s) 747, 930, 944
TspGWI ACGGA 3 cut(s) 120, 1095, 1143
TspRI CASTG 7 cut(s) 152, 301, 319, 556, 649, 774, 1222
Tth111I GACNNNGTC 1 cut(s) 597
VpaK11BI GGWCC 3 cut(s) 184, 681, 977
XagI CCTNNNNNAGG 1 cut(s) 612
XapI RAATTY 3 cut(s) 357, 627, 1177
XceI RCATGY 3 cut(s) 412, 726, 873
XmnI GAANNNNTTC 2 cut(s) 969, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.