Rroxscaffold_2G00120200

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005060
Physical Location & Seq
Forward (+)
52315487 .. 52325118
9632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120200.1

Sequence Viewer

Length: 1323 bp
ATGACGATGATGGCGAACTTGAATTCGAAATTAGGGTTGTTCCGATTAGGGACACGGTGGCTGAGCAGTACTGGGAGTGGGCCAGGGAAGAGGGTCGCGGCGGTTTGGGGCAATGGAGACTACGGGAGGCTCGGATTAGGGAGCTTGGACTCTCAGTGGAGCCCTACCCCTCTGCTCTGCTCTGCATTTCAGGACCAAAGCCTTGAAGCCGTTGCTTGCGGCGGAGCTCACACTCTGTTCTTGACCCAAGCTGGCCGGGTTTTCGCCACCGGCCTAAATGATTTCGGACAGCTTGGGGCCTCCGCTGATGTGCATTATGCTACTGAGCCGGTGGAGGTATCGGGAATCAACAAGCCAGTTGTGCAAATTTCCGCTGGATATTATCATTCTGGTGCTGTTACAGAAGATGGAGAGTTATACATGTGGGGGAAGAACTCATATGGGCAGCTTGGCCTTGGAAAGAGAGGAGCAAAAACAGTTCCTGCACCAGCAAAAGTTGAATGCTTGGCTGGAATCACCATTAGGATGGTAGCTTTGGGATCAGAGCACTCAGTGGCTGTTACTGATGGAGGTGAGTCCTTAAGTTGGGGAGGAGGTTCTGGAAGACTTGGTCATGGCCCTGAATCAGGCATTTTAGGAATTTTCAAAAGTACCAGTGAATATACACCAAGGCTTATCAAGAAGCTTGAGGGGACCAAAGTCAAAAAAGTTGCTGCTGGATTGCTGCATTCAGCATGCGTTGACGAAAATGGATCTGTATTCGTATTTGGTGAAAGAGCAGTAGATAGAATGGGGAGTTTAAAGAGGAAGAATGGAACTGCACTGTCCATGATCAGCAGACTGCCCTGCTCAGAAGAAGTTTCATGCGGTGGCTATCACACATGCGTCATAACAAGTGGTGGAGAACTCTACAGTTGGGGTTCAAATGAAAATGGTTGCCTTGGTATTGGGTATGTCCACTTGACAACTGTTTATTTAGTTACTGCTTTAAAGGTCAATTTTAGACTCTTAGTTTGCCGTACTACTGATGTACTTCATCTGCCGGAAAGAGTTCAAGGTCCATTCTTGAAATCTGCAGTTATCAAGGTATCTTGTGGTTGGAAGCATACAGCAGCAATTTCTGAAGGCAATATCTTCACATGGGGTTGGGGAGGTTCACATGGAACATTTTCTGAAGAAGGGCATTCTTCTGGAGGACAACTGGGTCAGGGAACTGATGTTGACTACATAAAACCTACGAGGGTCAAGTTTGGGGAGAATTTGAAAGCTTTACAGATATCATGTGGGTTCAATCACACTGGTGCCATACTAGAATACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

440

Amino Acids

46.45

Weight (kDa)

8.4

Isoelectric Point (pI)

26.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 34 - 433 4.2e-82 RCC1-like domain
RCC1 PF00415 36 - 81 2.7e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 70 - 97 1e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 85 - 133 5.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 120 - 149 1e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 136 - 187 2.5e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 194 - 247 7.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 287 - 313 1.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 301 - 373 3e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 395 - 436 1.9e-07 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1203
AccB1I GGYRCC 1 cut(s) 1301
AccII CGCG 1 cut(s) 98
AciI CCGC 7 cut(s) 98, 101, 219, 222, 303, 372, 867
AclWI GGATC 2 cut(s) 547, 760
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 4 cut(s) 22, 366, 639, 1258
AcuI CTGAAG 2 cut(s) 1143, 1194
AdeI CACNNNGTG 1 cut(s) 553
AfaI GTAC 4 cut(s) 70, 652, 1021, 1032
AfiI CCNNNNNNNGG 2 cut(s) 585, 626
AflII CTTAAG 1 cut(s) 580
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 9 cut(s) 22, 206, 500, 646, 924, 1055, 1069, 1264, 1291
AjnI CCWGG 1 cut(s) 82
AleI CACNNNNGTG 1 cut(s) 1299
AluBI AGCT 8 cut(s) 144, 227, 251, 292, 448, 533, 685, 1268
AluI AGCT 8 cut(s) 144, 227, 251, 292, 448, 533, 685, 1268
Alw21I GWGCWC 2 cut(s) 229, 549
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 2 cut(s) 547, 760
AlwNI CAGNNNCTG 2 cut(s) 482, 557
AoxI GGCC 6 cut(s) 80, 253, 271, 297, 451, 616
ApeKI GCWGC 4 cut(s) 445, 713, 724, 1112
ApoI RAATTY 4 cut(s) 22, 366, 639, 1258
ArsI GACNNNNNNTTYG 2 cut(s) 996, 1028
Asp700I GAANNNNTTC 2 cut(s) 1050, 1168
AspS9I GGNCC 6 cut(s) 80, 193, 297, 617, 693, 1058
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 3 cut(s) 508, 584, 782
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 3 cut(s) 193, 693, 1058
BanI GGYRCC 1 cut(s) 1301
BanII GRGCYC 2 cut(s) 164, 229
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 2 cut(s) 229, 549
BbvI GCAGC 4 cut(s) 457, 700, 711, 1124
BccI CCATC 4 cut(s) 4, 401, 520, 560
BceAI ACGGC 2 cut(s) 194, 1002
BciT130I CCWGG 1 cut(s) 84
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 1310
BfmI CTRYAG 2 cut(s) 910, 1074
BfrI CTTAAG 1 cut(s) 580
BisI GCNGC 6 cut(s) 99, 220, 446, 714, 725, 1113
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 6 cut(s) 100, 221, 447, 715, 726, 1114
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 2 cut(s) 84, 257
Bme18I GGWCC 3 cut(s) 193, 693, 1058
BmgT120I GGNCC 6 cut(s) 80, 193, 297, 617, 693, 1058
BmiI GGNNCC 4 cut(s) 161, 298, 694, 1303
BmrFI CCNGG 2 cut(s) 84, 257
BmrI ACTGGG 2 cut(s) 81, 1211
BmuI ACTGGG 2 cut(s) 81, 1211
BoxI GACNNNNGTC 1 cut(s) 698
BpiI GAAGAC 1 cut(s) 610
BpmI CTGGAG 1 cut(s) 1212
Bpu1102I GCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 257
BsaJI CCNNGG 4 cut(s) 83, 454, 668, 940
Bsc4I CCNNNNNNNGG 2 cut(s) 585, 626
Bse118I RCCGGY 2 cut(s) 269, 328
Bse1I ACTGG 5 cut(s) 76, 356, 654, 1206, 1303
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 4 cut(s) 83, 454, 668, 940
BseGI GGATG 1 cut(s) 531
BseLI CCNNNNNNNGG 2 cut(s) 585, 626
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 5 cut(s) 53, 167, 315, 564, 864
BseNI ACTGG 5 cut(s) 76, 356, 654, 1206, 1303
BseRI GAGGAG 2 cut(s) 480, 606
BseXI GCAGC 4 cut(s) 457, 700, 711, 1124
BsgI GTGCAG 2 cut(s) 468, 804
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 6 cut(s) 82, 255, 273, 299, 453, 618
BshNI GGYRCC 1 cut(s) 1301
BsiHKAI GWGCWC 2 cut(s) 229, 549
BsiSI CCGG 4 cut(s) 256, 270, 329, 1043
BslFI GGGAC 2 cut(s) 64, 706
BslI CCNNNNNNNGG 2 cut(s) 585, 626
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 64, 706
BsmI GAATGC 3 cut(s) 506, 727, 1183
BsnI GGCC 6 cut(s) 82, 255, 273, 299, 453, 618
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 164, 229, 549
Bsp143I GATC 3 cut(s) 539, 752, 831
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 7 cut(s) 98, 101, 219, 222, 303, 372, 867
BspANI GGCC 6 cut(s) 82, 255, 273, 299, 453, 618
BspCNI CTCAG 5 cut(s) 54, 166, 316, 563, 863
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 4 cut(s) 161, 298, 694, 1303
BspMAI CTGCAG 1 cut(s) 1078
BspPI GGATC 2 cut(s) 547, 760
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1301
BspTI CTTAAG 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 2 cut(s) 269, 328
BsrI ACTGG 5 cut(s) 76, 356, 654, 1206, 1303
BssAI RCCGGY 2 cut(s) 269, 328
BssECI CCNNGG 4 cut(s) 83, 454, 668, 940
BssMI GATC 3 cut(s) 539, 752, 831
BssT1I CCWWGG 3 cut(s) 454, 668, 940
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 5 cut(s) 57, 478, 825, 914, 970
Bst6I CTCTTC 1 cut(s) 83
BstAFI CTTAAG 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 217, 253, 736
BstDEI CTNAG 6 cut(s) 62, 153, 324, 550, 850, 1009
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 1 cut(s) 531
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 3 cut(s) 542, 755, 834
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 3 cut(s) 539, 752, 831
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 3 cut(s) 424, 738, 885
BstPAI GACNNNNGTC 1 cut(s) 698
BstSCI CCNGG 2 cut(s) 82, 255
BstSFI CTRYAG 2 cut(s) 910, 1074
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 4 cut(s) 457, 700, 711, 1124
BstV2I GAAGAC 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 752
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 1 cut(s) 752
BsuRI GGCC 6 cut(s) 82, 255, 273, 299, 453, 618
BtsCI GGATG 1 cut(s) 531
BtsIMutI CAGTG 5 cut(s) 161, 558, 661, 821, 1296
Cac8I GCNNGC 3 cut(s) 217, 253, 736
CaiI CAGNNNCTG 2 cut(s) 482, 557
Cfr10I RCCGGY 2 cut(s) 269, 328
Cfr13I GGNCC 6 cut(s) 80, 193, 297, 617, 693, 1058
CseI GACGC 1 cut(s) 874
Csp6I GTAC 4 cut(s) 69, 651, 1020, 1031
CviAII CATG 9 cut(s) 421, 614, 735, 829, 864, 882, 1140, 1160, 1281
CviQI GTAC 4 cut(s) 69, 651, 1020, 1031
DdeI CTNAG 6 cut(s) 62, 153, 324, 550, 850, 1009
DpnI GATC 3 cut(s) 541, 754, 833
DpnII GATC 3 cut(s) 539, 752, 831
DraI TTTAAA 2 cut(s) 801, 990
DraIII CACNNNGTG 1 cut(s) 553
DrdI GACNNNNNNGTC 1 cut(s) 1203
DseDI GACNNNNNNGTC 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 3 cut(s) 454, 668, 940
Eco24I GRGCYC 2 cut(s) 164, 229
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 3 cut(s) 193, 693, 1058
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 1143, 1194
EcoICRI GAGCTC 1 cut(s) 227
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 82
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 3 cut(s) 454, 668, 940
EcoT38I GRGCYC 2 cut(s) 164, 229
ErhI CCWWGG 3 cut(s) 454, 668, 940
FaeI CATG 9 cut(s) 424, 617, 738, 832, 867, 885, 1143, 1163, 1284
FaqI GGGAC 2 cut(s) 64, 706
FatI CATG 9 cut(s) 420, 613, 734, 828, 863, 881, 1139, 1159, 1280
FauNDI CATATG 1 cut(s) 439
FbaI TGATCA 1 cut(s) 831
Fnu4HI GCNGC 6 cut(s) 99, 220, 446, 714, 725, 1113
FokI GGATG 1 cut(s) 538
FriOI GRGCYC 2 cut(s) 164, 229
Fsp4HI GCNGC 6 cut(s) 99, 220, 446, 714, 725, 1113
FspBI CTAG 1 cut(s) 1310
GluI GCNGC 6 cut(s) 99, 220, 446, 714, 725, 1113
GsuI CTGGAG 1 cut(s) 1212
HaeIII GGCC 6 cut(s) 82, 255, 273, 299, 453, 618
HapII CCGG 4 cut(s) 256, 270, 329, 1043
HgaI GACGC 1 cut(s) 874
Hin1II CATG 9 cut(s) 424, 617, 738, 832, 867, 885, 1143, 1163, 1284
HincII GTYRAC 2 cut(s) 742, 1222
HindII GTYRAC 2 cut(s) 742, 1222
HindIII AAGCTT 2 cut(s) 683, 1266
HinfI GANTC 6 cut(s) 149, 345, 513, 575, 623, 1005
HpaII CCGG 4 cut(s) 256, 270, 329, 1043
HphI GGTGA 3 cut(s) 508, 584, 782
Hpy166II GTNNAC 4 cut(s) 742, 958, 1157, 1222
Hpy188I TCNGA 7 cut(s) 44, 134, 287, 544, 853, 1123, 1174
Hpy188III TCNNGA 7 cut(s) 191, 241, 342, 600, 679, 1066, 1191
Hpy8I GTNNAC 4 cut(s) 742, 958, 1157, 1222
HpyAV CCTTC 2 cut(s) 1118, 1172
HpyCH4III ACNGT 5 cut(s) 57, 478, 825, 914, 970
HpyCH4V TGCA 7 cut(s) 185, 313, 364, 485, 727, 821, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 6 cut(s) 62, 153, 324, 550, 850, 1009
Hsp92II CATG 9 cut(s) 424, 617, 738, 832, 867, 885, 1143, 1163, 1284
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 3 cut(s) 539, 752, 831
LmnI GCTCC 4 cut(s) 141, 159, 224, 467
Lsp1109I GCAGC 4 cut(s) 457, 700, 711, 1124
MaeI CTAG 1 cut(s) 1310
MaeIII GTNAC 3 cut(s) 397, 559, 979
MalI GATC 3 cut(s) 541, 754, 833
MboI GATC 3 cut(s) 539, 752, 831
MboII GAAGA 9 cut(s) 100, 416, 442, 615, 820, 866, 1126, 1179, 1187
MflI RGATCY 1 cut(s) 752
MhlI GDGCHC 3 cut(s) 164, 229, 549
MluCI AATT 7 cut(s) 22, 29, 366, 639, 997, 1116, 1258
MlyI GAGTC 3 cut(s) 143, 584, 999
MmeI TCCRAC 1 cut(s) 1079
MroXI GAANNNNTTC 2 cut(s) 1050, 1168
MseI TTAA 3 cut(s) 581, 800, 989
MslI CAYNNNNRTG 3 cut(s) 390, 524, 1299
MspA1I CMGCKG 2 cut(s) 305, 374
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 4 cut(s) 256, 270, 329, 1043
MspR9I CCNGG 2 cut(s) 84, 257
Mva1269I GAATGC 3 cut(s) 506, 727, 1183
MvaI CCWGG 1 cut(s) 84
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 257
NdeI CATATG 1 cut(s) 439
NdeII GATC 3 cut(s) 539, 752, 831
NlaIII CATG 9 cut(s) 424, 617, 738, 832, 867, 885, 1143, 1163, 1284
NlaIV GGNNCC 4 cut(s) 161, 298, 694, 1303
NspI RCATGY 3 cut(s) 424, 738, 885
NspV TTCGAA 1 cut(s) 26
OliI CACNNNNGTG 1 cut(s) 1299
PaeI GCATGC 1 cut(s) 738
PciI ACATGT 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 3 cut(s) 506, 727, 1183
PdmI GAANNNNTTC 2 cut(s) 1050, 1168
PfeI GAWTC 3 cut(s) 345, 513, 623
PflFI GACNNNGTC 1 cut(s) 609
PkrI GCNGC 6 cut(s) 100, 221, 447, 715, 726, 1114
PleI GAGTC 3 cut(s) 143, 583, 999
PpsI GAGTC 3 cut(s) 143, 583, 999
PscI ACATGT 1 cut(s) 420
PshAI GACNNNNGTC 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 4 cut(s) 161, 298, 694, 1303
PspPI GGNCC 6 cut(s) 80, 193, 297, 617, 693, 1058
PstI CTGCAG 1 cut(s) 1078
PstNI CAGNNNCTG 2 cut(s) 482, 557
PsuI RGATCY 1 cut(s) 752
PsyI GACNNNGTC 1 cut(s) 609
RsaI GTAC 4 cut(s) 70, 652, 1021, 1032
RsaNI GTAC 4 cut(s) 69, 651, 1020, 1031
RseI CAYNNNNRTG 3 cut(s) 390, 524, 1299
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 3 cut(s) 581, 800, 989
SatI GCNGC 6 cut(s) 99, 220, 446, 714, 725, 1113
Sau3AI GATC 3 cut(s) 539, 752, 831
Sau96I GGNCC 6 cut(s) 80, 193, 297, 617, 693, 1058
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 3 cut(s) 143, 584, 999
ScrFI CCNGG 2 cut(s) 84, 257
SduI GDGCHC 3 cut(s) 164, 229, 549
SfcI CTRYAG 2 cut(s) 910, 1074
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 3 cut(s) 193, 693, 1058
SmiMI CAYNNNNRTG 3 cut(s) 390, 524, 1299
SmlI CTYRAG 2 cut(s) 580, 686
SmoI CTYRAG 2 cut(s) 580, 686
SphI GCATGC 1 cut(s) 738
Sse9I AATT 7 cut(s) 22, 29, 366, 639, 997, 1116, 1258
SsiI CCGC 7 cut(s) 98, 101, 219, 222, 303, 372, 867
SspMI CTAG 1 cut(s) 1310
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 82, 255
StyI CCWWGG 3 cut(s) 454, 668, 940
TaaI ACNGT 5 cut(s) 57, 478, 825, 914, 970
TaqI TCGA 1 cut(s) 26
TasI AATT 7 cut(s) 22, 29, 366, 639, 997, 1116, 1258
TatI WGTACW 2 cut(s) 68, 1030
TauI GCSGC 2 cut(s) 101, 222
TfiI GAWTC 3 cut(s) 345, 513, 623
Tru1I TTAA 3 cut(s) 581, 800, 989
Tru9I TTAA 3 cut(s) 581, 800, 989
TscAI CASTG 5 cut(s) 161, 558, 661, 828, 1303
TseI GCWGC 4 cut(s) 445, 713, 724, 1112
TspDTI ATGAA 3 cut(s) 852, 942, 1025
TspRI CASTG 5 cut(s) 161, 558, 661, 828, 1303
Tth111I GACNNNGTC 1 cut(s) 609
Vha464I CTTAAG 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 193, 693, 1058
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 4 cut(s) 22, 366, 639, 1258
XceI RCATGY 3 cut(s) 424, 738, 885
XmnI GAANNNNTTC 2 cut(s) 1050, 1168
XspI CTAG 1 cut(s) 1310
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.