MD00G1127500.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005060
Physical Location & Seq
Reverse (-)
27030269 .. 27041724
11456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1127500.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGTGGAGGACGATGAGAATGAAATTGGGGGGTACAGGCGGCGGAAGAAGGTGGTTGAGCAGTCGGAGTGATTGGGGGAAGGGGAAGAGGATCGCGGCGGTGTGGGGGAACGGGGACTACGGGAGGCTTGGGCTCGGTAGCCTGGGCTCTCAGTGGAAGCCTGCCCCTTTGCGTTCGTCTGCCTTTGGCGACCAATCCCTTGAAGCCATAGCTTGCGGCGGCGCTCACACTCTCTTCTTAACGGGCAGCGGCCGCCTTTACGCCACCGGTCTCAATGATTTTGGACAGCTTGGAGTTGGGGATTCTCATGATTTAACTTACACTTGTAAGCCCTTAGAGGTAACTGGAATTGGCAAGGAAATTGTTCGAATTTCGGCCGGTTACTACCACTCTTGTGCTATTACAGAAGATGGAGAATTGTACATGTGGGGGAAGAACTCAAATGGGCAGCTTGGTCTTGGAAAAAGGGCTGCAGGGCTAATTTCTGTACCAACCAAAGTTGAATGCTTGGCTGGAATCACCATTAATACAGCAGCTTTGGGATCAGAGCACTCAGTGGCTGTTACTGATGGAGGTGAGTCCTTAAGTTGGGGAGGAGGGTCTGGAAGACTTGGTCATGGCCACGAATCAGGAATTTTAGGAATTCTCAGAAGTACCAGCGAATATACACCACGACTTATCAAGAAACTTGAGGGGATCAAGGTTAAAGAGGTTGCTGCTGGATTGCTGCAATCAGCATGCATTGATGACAATGGGTCCGTATTCATGTTTGGTGAAAGAACAGTAGATAAATTGTGGTCTGGAAAGGGGAACAATGCAACAACACCATCCATGATCAGCAAACTGCCATGCTCCAAAGAAGTTGCTTGTGGTGGCTATCACACGTGTGTCTTAACAAGTGGTGGAGAACTGTACAGTTGGGGATCAAATGAAAATGGGTGCCTTGGCATTGGTACGACAGACGTTTTTCACCACCCAGAAAGAGTTCAGGGACCTTTTTTAAAATCCCCGGTTGTCAAGGTATCGTGCGGTTGGAAGCATACAGCAGCAATTTCTGAAGGCAATATTTATACATGGGGTTGGGGAGGTTCCCTTGGAACATTTTCCGAAGATGGACATTCTTCTGGAGGACAACTGGGTCATGGAACTGATGTTGACTGCATAAAACCTGCAATGGTTACGTTCGGGCAGAATGTGAAAGCACTACAAATATCATGTGGATTCAATCATACTGGTGCCATACTTGAATACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

419

Amino Acids

43.93

Weight (kDa)

8.2

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 33 - 411 2.6e-82 RCC1-like domain
RCC1 PF00415 35 - 80 1.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 69 - 96 2.6e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 83 - 134 5.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 121 - 150 5.7e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 137 - 188 6.2e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 287 - 314 2.1e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 302 - 351 1.9e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 373 - 414 1.6e-06 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1137
Acc36I ACCTGC 1 cut(s) 1177
AccB1I GGYRCC 2 cut(s) 939, 1235
AccII CGCG 1 cut(s) 95
AciI CCGC 9 cut(s) 39, 42, 95, 98, 216, 219, 249, 253, 1029
AclWI GGATC 4 cut(s) 98, 550, 704, 931
AcoI YGGCCR 3 cut(s) 250, 375, 619
AcsI RAATTY 3 cut(s) 369, 633, 642
AcuI CTGAAG 1 cut(s) 1077
AcvI CACGTG 1 cut(s) 885
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 6 cut(s) 34, 422, 489, 655, 914, 955
AfiI CCNNNNNNNGG 1 cut(s) 588
AflII CTTAAG 1 cut(s) 583
AflIII ACRYGT 3 cut(s) 423, 882, 884
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 4 cut(s) 203, 503, 1225, 1247
AhdI GACNNNNNGTC 1 cut(s) 754
AjnI CCWGG 1 cut(s) 141
AleI CACNNNNGTG 2 cut(s) 393, 885
AluBI AGCT 4 cut(s) 212, 289, 451, 536
AluI AGCT 4 cut(s) 212, 289, 451, 536
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 4 cut(s) 98, 550, 704, 931
AlwNI CAGNNNCTG 1 cut(s) 560
AoxI GGCC 3 cut(s) 250, 375, 619
ApeKI GCWGC 7 cut(s) 246, 448, 470, 533, 716, 727, 1046
ApoI RAATTY 3 cut(s) 369, 633, 642
AseI ATTAAT 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 266
Asp700I GAANNNNTTC 3 cut(s) 363, 984, 1102
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 756, 992
AsuC2I CCSGG 1 cut(s) 1010
AsuHPI GGTGA 4 cut(s) 511, 587, 785, 962
AsuII TTCGAA 1 cut(s) 367
AvaII GGWCC 2 cut(s) 756, 992
BalI TGGCCA 1 cut(s) 621
BanI GGYRCC 2 cut(s) 939, 1235
BanII GRGCYC 2 cut(s) 135, 149
BbrPI CACGTG 1 cut(s) 885
BbsI GAAGAC 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 7 cut(s) 258, 457, 460, 545, 703, 714, 1058
BccI CCATC 4 cut(s) 404, 563, 835, 1106
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 834
BcnI CCSGG 1 cut(s) 1010
BcoDI GTCTC 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 583
BfuAI ACCTGC 1 cut(s) 1177
Bme1390I CCNGG 2 cut(s) 143, 1010
Bme18I GGWCC 2 cut(s) 756, 992
BmeRI GACNNNNNGTC 1 cut(s) 754
BmgT120I GGNCC 2 cut(s) 756, 992
BmiI GGNNCC 5 cut(s) 757, 941, 993, 1090, 1237
BmrFI CCNGG 2 cut(s) 143, 1010
BmrI ACTGGG 1 cut(s) 1145
BmuI ACTGGG 1 cut(s) 1145
BpiI GAAGAC 1 cut(s) 613
BpmI CTGGAG 1 cut(s) 1146
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 710
BpuMI CCSGG 1 cut(s) 1010
BsaAI YACGTR 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 142, 943, 1008, 1093
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 2 cut(s) 266, 377
Bse1I ACTGG 3 cut(s) 349, 1140, 1237
Bse3DI GCAATG 1 cut(s) 1179
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 4 cut(s) 142, 943, 1008, 1093
BseGI GGATG 1 cut(s) 827
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMI GCAATG 1 cut(s) 1179
BseMII CTCAG 3 cut(s) 164, 567, 661
BseNI ACTGG 3 cut(s) 349, 1140, 1237
BseRI GAGGAG 1 cut(s) 609
BseX3I CGGCCG 2 cut(s) 250, 375
BseXI GCAGC 7 cut(s) 258, 457, 460, 545, 703, 714, 1058
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 2 cut(s) 253, 378
BshFI GGCC 3 cut(s) 252, 377, 621
BshNI GGYRCC 2 cut(s) 939, 1235
BshTI ACCGGT 1 cut(s) 266
BsiEI CGRYCG 2 cut(s) 253, 378
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 3 cut(s) 267, 378, 1010
BslFI GGGAC 2 cut(s) 128, 1005
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 128, 1005
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 3 cut(s) 252, 377, 621
Bso31I GGTCTC 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 3 cut(s) 135, 149, 552
Bsp1407I TGTACA 2 cut(s) 420, 912
Bsp143I GATC 5 cut(s) 90, 542, 696, 834, 923
BspACI CCGC 9 cut(s) 39, 42, 95, 98, 216, 219, 249, 253, 1029
BspANI GGCC 3 cut(s) 252, 377, 621
BspCNI CTCAG 3 cut(s) 163, 566, 660
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 5 cut(s) 757, 941, 993, 1090, 1237
BspMAI CTGCAG 1 cut(s) 475
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 4 cut(s) 98, 550, 704, 931
BspT104I TTCGAA 1 cut(s) 367
BspT107I GGYRCC 2 cut(s) 939, 1235
BspTI CTTAAG 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 1179
BsrFI RCCGGY 2 cut(s) 266, 377
BsrGI TGTACA 2 cut(s) 420, 912
BsrI ACTGG 3 cut(s) 349, 1140, 1237
BssAI RCCGGY 2 cut(s) 266, 377
BssECI CCNNGG 4 cut(s) 142, 943, 1008, 1093
BssMI GATC 5 cut(s) 90, 542, 696, 834, 923
BssT1I CCWWGG 2 cut(s) 943, 1093
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 3 cut(s) 784, 912, 917
Bst6I CTCTTC 2 cut(s) 80, 239
BstAFI CTTAAG 1 cut(s) 583
BstAUI TGTACA 2 cut(s) 420, 912
BstBAI YACGTR 1 cut(s) 885
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 3 cut(s) 162, 214, 739
BstDEI CTNAG 4 cut(s) 150, 334, 553, 647
BstF5I GGATG 1 cut(s) 827
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 5 cut(s) 93, 545, 699, 837, 926
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 5 cut(s) 90, 542, 696, 834, 923
BstMCI CGRYCG 2 cut(s) 253, 378
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 2 cut(s) 427, 741
BstSCI CCNGG 2 cut(s) 141, 1008
BstSFI CTRYAG 1 cut(s) 471
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 7 cut(s) 258, 457, 460, 545, 703, 714, 1058
BstV2I GAAGAC 1 cut(s) 613
BstZI CGGCCG 2 cut(s) 250, 375
BsuRI GGCC 3 cut(s) 252, 377, 621
BtsCI GGATG 1 cut(s) 827
BtsIMutI CAGTG 2 cut(s) 158, 561
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 3 cut(s) 162, 214, 739
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 307
CciNI GCGGCCGC 1 cut(s) 250
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 2 cut(s) 266, 377
Cfr13I GGNCC 2 cut(s) 756, 992
Csp6I GTAC 6 cut(s) 33, 421, 488, 654, 913, 954
CspAI ACCGGT 1 cut(s) 266
CviQI GTAC 6 cut(s) 33, 421, 488, 654, 913, 954
DdeI CTNAG 4 cut(s) 150, 334, 553, 647
DpnI GATC 5 cut(s) 92, 544, 698, 836, 925
DpnII GATC 5 cut(s) 90, 542, 696, 834, 923
DraI TTTAAA 1 cut(s) 1002
DraIII CACNNNGTG 1 cut(s) 556
DrdI GACNNNNNNGTC 1 cut(s) 1137
DriI GACNNNNNGTC 1 cut(s) 754
DseDI GACNNNNNNGTC 1 cut(s) 1137
EaeI YGGCCR 3 cut(s) 250, 375, 619
EagI CGGCCG 2 cut(s) 250, 375
Eam1104I CTCTTC 2 cut(s) 80, 239
Eam1105I GACNNNNNGTC 1 cut(s) 754
EarI CTCTTC 2 cut(s) 80, 239
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 2 cut(s) 250, 375
Eco130I CCWWGG 2 cut(s) 943, 1093
Eco24I GRGCYC 2 cut(s) 135, 149
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 756, 992
Eco52I CGGCCG 2 cut(s) 250, 375
Eco57I CTGAAG 1 cut(s) 1077
Eco72I CACGTG 1 cut(s) 885
EcoO109I RGGNCCY 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 943, 1093
EcoT22I ATGCAT 1 cut(s) 743
EcoT38I GRGCYC 2 cut(s) 135, 149
ErhI CCWWGG 2 cut(s) 943, 1093
FaqI GGGAC 2 cut(s) 128, 1005
FbaI TGATCA 1 cut(s) 834
FokI GGATG 1 cut(s) 814
FriOI GRGCYC 2 cut(s) 135, 149
GlaI GCGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 1146
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 252, 377, 621
HapII CCGG 3 cut(s) 267, 378, 1010
HhaI GCGC 1 cut(s) 224
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HinfI GANTC 5 cut(s) 302, 516, 578, 626, 1221
HpaII CCGG 3 cut(s) 267, 378, 1010
HphI GGTGA 4 cut(s) 511, 587, 785, 962
Hpy166II GTNNAC 1 cut(s) 1156
Hpy188I TCNGA 5 cut(s) 66, 547, 650, 1057, 1108
Hpy188III TCNNGA 6 cut(s) 308, 603, 630, 682, 801, 1125
Hpy8I GTNNAC 1 cut(s) 1156
HpyAV CCTTC 3 cut(s) 42, 73, 1052
HpyCH4III ACNGT 3 cut(s) 784, 912, 917
HpyCH4IV ACGT 3 cut(s) 884, 963, 1181
HpyCH4V TGCA 6 cut(s) 473, 730, 741, 818, 1161, 1172
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 4 cut(s) 150, 334, 553, 647
HpySE526I ACGT 3 cut(s) 884, 963, 1181
HspAI GCGC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 5 cut(s) 90, 542, 696, 834, 923
LmnI GCTCC 1 cut(s) 857
Lsp1109I GCAGC 7 cut(s) 258, 457, 460, 545, 703, 714, 1058
MaeII ACGT 3 cut(s) 884, 963, 1181
MaeIII GTNAC 4 cut(s) 340, 380, 562, 1177
MalI GATC 5 cut(s) 92, 544, 698, 836, 925
MboI GATC 5 cut(s) 90, 542, 696, 834, 923
MboII GAAGA 8 cut(s) 57, 97, 226, 419, 445, 618, 1113, 1121
MhlI GDGCHC 3 cut(s) 135, 149, 552
MlsI TGGCCA 1 cut(s) 621
MluNI TGGCCA 1 cut(s) 621
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 2 cut(s) 44, 1013
Mox20I TGGCCA 1 cut(s) 621
Mph1103I ATGCAT 1 cut(s) 743
MroXI GAANNNNTTC 3 cut(s) 363, 984, 1102
MscI TGGCCA 1 cut(s) 621
MseI TTAA 7 cut(s) 239, 314, 525, 584, 705, 893, 1001
MslI CAYNNNNRTG 3 cut(s) 393, 885, 1233
Msp20I TGGCCA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 3 cut(s) 267, 378, 1010
MspR9I CCNGG 2 cut(s) 143, 1010
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 252
NciI CCSGG 1 cut(s) 1010
NdeII GATC 5 cut(s) 90, 542, 696, 834, 923
NlaIV GGNNCC 5 cut(s) 757, 941, 993, 1090, 1237
NotI GCGGCCGC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 743
NspI RCATGY 2 cut(s) 427, 741
NspV TTCGAA 1 cut(s) 367
OliI CACNNNNGTG 2 cut(s) 393, 885
PaeI GCATGC 1 cut(s) 741
PagI TCATGA 1 cut(s) 307
PciI ACATGT 1 cut(s) 423
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 3 cut(s) 363, 984, 1102
PfeI GAWTC 4 cut(s) 302, 516, 626, 1221
PflFI GACNNNGTC 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 266
PleI GAGTC 1 cut(s) 586
PmaCI CACGTG 1 cut(s) 885
PmlI CACGTG 1 cut(s) 885
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 885
PpuMI RGGWCCY 1 cut(s) 992
PscI ACATGT 1 cut(s) 423
PshBI ATTAAT 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 1 cut(s) 141
PspCI CACGTG 1 cut(s) 885
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 5 cut(s) 757, 941, 993, 1090, 1237
PspPI GGNCC 2 cut(s) 756, 992
PspPPI RGGWCCY 1 cut(s) 992
PsrI GAACNNNNNNTAC 2 cut(s) 101, 133
PstI CTGCAG 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 560
PsyI GACNNNGTC 1 cut(s) 612
RsaI GTAC 6 cut(s) 34, 422, 489, 655, 914, 955
RsaNI GTAC 6 cut(s) 33, 421, 488, 654, 913, 954
RseI CAYNNNNRTG 3 cut(s) 393, 885, 1233
SaqAI TTAA 7 cut(s) 239, 314, 525, 584, 705, 893, 1001
Sau3AI GATC 5 cut(s) 90, 542, 696, 834, 923
Sau96I GGNCC 2 cut(s) 756, 992
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 2 cut(s) 143, 1010
SduI GDGCHC 3 cut(s) 135, 149, 552
SfcI CTRYAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 367
SinI GGWCC 2 cut(s) 756, 992
SmiMI CAYNNNNRTG 3 cut(s) 393, 885, 1233
SmlI CTYRAG 2 cut(s) 583, 689
SmoI CTYRAG 2 cut(s) 583, 689
SphI GCATGC 1 cut(s) 741
SsiI CCGC 9 cut(s) 39, 42, 95, 98, 216, 219, 249, 253, 1029
SspI AATATT 1 cut(s) 1066
StyD4I CCNGG 2 cut(s) 141, 1008
StyI CCWWGG 2 cut(s) 943, 1093
TaaI ACNGT 3 cut(s) 784, 912, 917
TaiI ACGT 3 cut(s) 887, 966, 1184
TaqI TCGA 1 cut(s) 367
TatI WGTACW 2 cut(s) 420, 912
TauI GCSGC 6 cut(s) 42, 98, 219, 222, 252, 255
TfiI GAWTC 4 cut(s) 302, 516, 626, 1221
Tru1I TTAA 7 cut(s) 239, 314, 525, 584, 705, 893, 1001
Tru9I TTAA 7 cut(s) 239, 314, 525, 584, 705, 893, 1001
TscAI CASTG 2 cut(s) 158, 561
TseI GCWGC 7 cut(s) 246, 448, 470, 533, 716, 727, 1046
TspDTI ATGAA 3 cut(s) 35, 754, 945
TspGWI ACGGA 1 cut(s) 748
TspRI CASTG 2 cut(s) 158, 561
Tth111I GACNNNGTC 1 cut(s) 612
Vha464I CTTAAG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 756, 992
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 3 cut(s) 369, 633, 642
XceI RCATGY 2 cut(s) 427, 741
XmnI GAANNNNTTC 3 cut(s) 363, 984, 1102
Zsp2I ATGCAT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.