FvH4_1g27810

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002535
Physical Location & Seq
Forward (+)
19380835 .. 19383962
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27810.t1

Sequence Viewer

Length: 813 bp
ATGAATAACCGAGTCCTCTTGCTTGGAGTTGGCACCACCATTCGCGCTAATCGCTGCTTCTTCCCACACCGCTTCTTCACTTGCAGGACCATTCAGATGGAAACCCGTAAGGTTGAGGTGGTGGTGGCCGACCCTCACCTGTTGGATGCCAAAATTGCCGCCATTCGGAATGCGGGTCCCCCCAAACTTAAGGTTATAGCAGATTTTGATGCCACACTGACCAAGTACAGGGTCAATGGCTGTCGGGCACAAAGCAGTCATGGTCTTTTGCAACAGGAGAATCCTGAATATGATAACAAGAGGCGTCAGTTATACGAATACTATCATCCGTTAGAGTTTTCCCCAACTATTCCAATTCAGCAGAAAACTCTGCTTACGGAAGAATGGCAAGTTTTACCATACACTGATTATTTCCCACTTGCAGAAAAGAGACATTCCCGTTCTTATATTTCTGCAGGACTTGCGGATATCATTGAGGAGGTCCTGAGGCAGAAAGTTCACAGAGTATTCAAAAATGTGAAGATCGTCTCCAACAGGATGGTATTTGATGAAAACGGCCGCCTTGTATCTTTTAAAGGGAAGACGATACATAGTCTGAATAAAAATGAGCATGCTCTTGATATGGCTGCTCCACTTCATGAGCAGCTAGGTGATTCTGACGGACCAACTTATGAGAATGTGTCAGTCAAGACAAGAAGAAATATTATTCTTTTGGGTGATCATATTGGTGACCTGGGGATGTCTGATGGTTTGAACTATGAGAATAGAATTTCTGTGGGATTTCTGGTCCTGGAAATGGGATCTAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

271

Amino Acids

30.85

Weight (kDa)

9.02

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 72 - 129 4.6e-13 Pyrimidine 5'-nucleotidase (UMPH-1)
UMPH-1 PF05822 150 - 263 1.8e-35 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccII CGCG 1 cut(s) 45
AciI CCGC 5 cut(s) 70, 159, 173, 464, 559
AclWI GGATC 1 cut(s) 808
AcoI YGGCCR 2 cut(s) 126, 556
AcsI RAATTY 1 cut(s) 768
AcyI GRCGYC 1 cut(s) 304
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 165, 228, 796
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 2 cut(s) 511, 754
AjnI CCWGG 2 cut(s) 732, 789
AluBI AGCT 1 cut(s) 646
AluI AGCT 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 424, 532
AlwI GGATC 1 cut(s) 808
AoxI GGCC 2 cut(s) 126, 556
ApeKI GCWGC 3 cut(s) 54, 626, 643
ApoI RAATTY 1 cut(s) 768
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 5 cut(s) 87, 176, 481, 662, 787
AsuHPI GGTGA 4 cut(s) 128, 662, 728, 740
AvaII GGWCC 5 cut(s) 87, 176, 481, 662, 787
AxyI CCTNAGG 1 cut(s) 485
BaeGI GKGCMC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 41, 613, 655
BccI CCATC 3 cut(s) 91, 532, 740
BceAI ACGGC 1 cut(s) 571
BciT130I CCWGG 2 cut(s) 734, 791
BclI TGATCA 1 cut(s) 718
BcoDI GTCTC 2 cut(s) 424, 532
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 453
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 5 cut(s) 55, 159, 559, 627, 644
BlsI GCNGC 5 cut(s) 56, 160, 560, 628, 645
Bme1390I CCNGG 2 cut(s) 734, 791
Bme18I GGWCC 5 cut(s) 87, 176, 481, 662, 787
BmgT120I GGNCC 5 cut(s) 87, 176, 481, 662, 787
BmiI GGNNCC 3 cut(s) 34, 177, 178
BmrFI CCNGG 2 cut(s) 734, 791
BmsI GCATC 2 cut(s) 136, 199
BpiI GAAGAC 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 733
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 228, 796
Bse21I CCTNAGG 1 cut(s) 485
BseBI CCWGG 2 cut(s) 734, 791
BseDI CCNNGG 1 cut(s) 733
BseGI GGATG 4 cut(s) 151, 325, 543, 744
BseLI CCNNNNNNNGG 3 cut(s) 165, 228, 796
BseMII CTCAG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 250
BseX3I CGGCCG 1 cut(s) 556
BseXI GCAGC 3 cut(s) 41, 613, 655
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 2 cut(s) 128, 558
BshNI GGYRCC 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 559
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 165, 228, 796
BsmAI GTCTC 2 cut(s) 424, 532
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 2 cut(s) 128, 558
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 3 cut(s) 522, 718, 800
BspACI CCGC 5 cut(s) 70, 159, 173, 464, 559
BspANI GGCC 2 cut(s) 128, 558
BspCNI CTCAG 1 cut(s) 477
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 3 cut(s) 34, 177, 178
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 733
BssMI GATC 3 cut(s) 522, 718, 800
BssNI GRCGYC 1 cut(s) 304
Bst2UI CCWGG 2 cut(s) 734, 791
BstACI GRCGYC 1 cut(s) 304
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 1 cut(s) 485
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 4 cut(s) 151, 325, 543, 744
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 3 cut(s) 525, 721, 803
BstMAI GTCTC 2 cut(s) 424, 532
BstMBI GATC 3 cut(s) 522, 718, 800
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 3 cut(s) 51, 155, 461
BstNI CCWGG 2 cut(s) 734, 791
BstNSI RCATGY 1 cut(s) 614
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 732, 789
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 41, 613, 655
BstV2I GAAGAC 1 cut(s) 587
BstX2I RGATCY 1 cut(s) 800
BstXI CCANNNNNNTGG 2 cut(s) 97, 538
BstYI RGATCY 1 cut(s) 800
BstZI CGGCCG 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 485
BsuRI GGCC 2 cut(s) 128, 558
BtsCI GGATG 4 cut(s) 151, 325, 543, 744
BtsIMutI CAGTG 2 cut(s) 215, 402
Cac8I GCNNGC 1 cut(s) 612
CciI TCATGA 1 cut(s) 637
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 5 cut(s) 87, 176, 481, 662, 787
CseI GACGC 1 cut(s) 293
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 260, 611, 638
CviJI RGCY 5 cut(s) 128, 240, 558, 626, 646
CviKI_1 RGCY 5 cut(s) 128, 240, 558, 626, 646
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 485
DpnI GATC 3 cut(s) 524, 720, 802
DpnII GATC 3 cut(s) 522, 718, 800
DraI TTTAAA 1 cut(s) 574
EaeI YGGCCR 2 cut(s) 126, 556
EagI CGGCCG 1 cut(s) 556
EclXI CGGCCG 1 cut(s) 556
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 5 cut(s) 87, 176, 481, 662, 787
Eco52I CGGCCG 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 485
Eco91I GGTNACC 1 cut(s) 728
EcoO109I RGGNCCY 2 cut(s) 176, 481
EcoO65I GGTNACC 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 732, 789
EcoRV GATATC 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 3 cut(s) 263, 614, 641
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 259, 610, 637
FauI CCCGC 1 cut(s) 166
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 5 cut(s) 55, 159, 559, 627, 644
FokI GGATG 4 cut(s) 158, 312, 550, 751
Fsp4HI GCNGC 5 cut(s) 55, 159, 559, 627, 644
FspBI CTAG 1 cut(s) 647
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 55, 159, 559, 627, 644
HaeIII GGCC 2 cut(s) 128, 558
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 304
Hin1II CATG 3 cut(s) 263, 614, 641
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 3 cut(s) 12, 280, 653
HphI GGTGA 4 cut(s) 128, 662, 728, 740
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 5 cut(s) 96, 168, 597, 658, 745
Hpy188III TCNNGA 5 cut(s) 284, 484, 617, 638, 688
Hpy8I GTNNAC 1 cut(s) 499
HpyCH4V TGCA 4 cut(s) 84, 271, 422, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 155, 461
HpyF3I CTNAG 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 304
Hsp92II CATG 3 cut(s) 263, 614, 641
HspAI GCGC 1 cut(s) 45
KflI GGGWCCC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 3 cut(s) 522, 718, 800
LmnI GCTCC 1 cut(s) 634
Lsp1109I GCAGC 3 cut(s) 41, 613, 655
LweI GCATC 2 cut(s) 136, 199
MaeI CTAG 1 cut(s) 647
MaeIII GTNAC 1 cut(s) 728
MalI GATC 3 cut(s) 524, 720, 802
MboI GATC 3 cut(s) 522, 718, 800
MboII GAAGA 6 cut(s) 52, 67, 392, 532, 592, 708
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 3 cut(s) 153, 354, 768
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 2 cut(s) 123, 555
MnlI CCTC 7 cut(s) 26, 109, 144, 294, 469, 472, 480
MseI TTAA 2 cut(s) 189, 573
MslI CAYNNNNRTG 2 cut(s) 95, 726
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 734, 791
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 2 cut(s) 734, 791
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 51, 155, 461
NdeII GATC 3 cut(s) 522, 718, 800
NlaIII CATG 3 cut(s) 263, 614, 641
NlaIV GGNNCC 3 cut(s) 34, 177, 178
NmuCI GTSAC 1 cut(s) 728
NspI RCATGY 1 cut(s) 614
PaeI GCATGC 1 cut(s) 614
PagI TCATGA 1 cut(s) 637
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 280, 653
PfoI TCCNGGA 1 cut(s) 789
PkrI GCNGC 5 cut(s) 56, 160, 560, 628, 645
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 2 cut(s) 176, 481
Psp5II RGGWCCY 2 cut(s) 176, 481
Psp6I CCWGG 2 cut(s) 732, 789
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 2 cut(s) 732, 789
PspN4I GGNNCC 3 cut(s) 34, 177, 178
PspPI GGNCC 5 cut(s) 87, 176, 481, 662, 787
PspPPI RGGWCCY 2 cut(s) 176, 481
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 800
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 2 cut(s) 95, 726
SaqAI TTAA 2 cut(s) 189, 573
SatI GCNGC 5 cut(s) 55, 159, 559, 627, 644
Sau3AI GATC 3 cut(s) 522, 718, 800
Sau96I GGNCC 5 cut(s) 87, 176, 481, 662, 787
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 2 cut(s) 734, 791
SduI GDGCHC 1 cut(s) 250
SetI ASST 8 cut(s) 114, 120, 141, 195, 483, 648, 652, 735
SfaNI GCATC 2 cut(s) 136, 199
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 5 cut(s) 87, 176, 481, 662, 787
SmiMI CAYNNNNRTG 2 cut(s) 95, 726
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 614
Sse9I AATT 3 cut(s) 153, 354, 768
SsiI CCGC 5 cut(s) 70, 159, 173, 464, 559
SspI AATATT 1 cut(s) 703
SspMI CTAG 1 cut(s) 647
StyD4I CCNGG 2 cut(s) 732, 789
TasI AATT 3 cut(s) 153, 354, 768
TatI WGTACW 1 cut(s) 225
TauI GCSGC 2 cut(s) 161, 561
TfiI GAWTC 2 cut(s) 280, 653
Tru1I TTAA 2 cut(s) 189, 573
Tru9I TTAA 2 cut(s) 189, 573
TscAI CASTG 2 cut(s) 222, 409
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 3 cut(s) 54, 626, 643
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 3 cut(s) 17, 564, 626
TspGWI ACGGA 3 cut(s) 318, 392, 675
TspRI CASTG 2 cut(s) 222, 409
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 5 cut(s) 87, 176, 481, 662, 787
XapI RAATTY 1 cut(s) 768
XceI RCATGY 1 cut(s) 614
XspI CTAG 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.