RchiOBHm_Chr1g0332191

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002535
Physical Location & Seq
Forward (+)
23286208 .. 23291884
5677 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56111

Sequence Viewer

Length: 1014 bp
ATGAGTAATCGCCTCCTCTTCGTTGGCACCACCATTTGCTCTAATCGCTGCTTCATCCCACACCACTTCACTTCCAGGAGCTGCTTTTGTAGTGGTACCTTGAGAAATCAGACTAACATGGAATCCGATGAGGTTGTGGTGGTGGCTCACCCTCACCTTTTGGATACCAAAATTGCTGCTATTCGGAATGCGGGTCCCCCCAAACTCCAGGTTATAGCAGATTTTGATGCCACACTCACAAAGTACAGAGTGAATGGTTCTCGGGGACAAAGCAGTCATGGCCTTTTGCAACAGGAGAATCCTGAATATAATAACAAAAGGCGGGAGCTATTCGAATACTATCATCCATTAGAGTTCTCGCCAGCTATTCCAATTGAACAAAAAACTAAGCTTATGGAGGAATGGTGGGGAAAGACTCATTCTCTTTTGATTGAGGGAGGCCTTACCTATGATGGGATAAGGCAATCCGTTGCTGATTCCACAATTGCTTTTAGGGAGGGTGTTGTTGAATTATTTGAGTTTTTAGAGAAAAGAGACATTCCTGTTCTTATATTTTCAGCAGGACTTGCAGATATCATAGAGGAGGTCCTGAGGCAGAAAGTTCACAGATTGTTTAAAAATGTGAAGATCGTCTCCAATAGGATGGTATTTGATGATAACAGCCACCTTGTATCGTTTAAAGGGAAGACGATTCATAGTCTAAATAAAAATGAGCACGCTCTTGATATGGCTGCTCCACTTCATGAGCAATTAGGTGATTTAGATGGGCCAACTTATGAGAATGCGTCAGTCAAGACAAGAAGAAATGTCATTCTTCTGGGCGATCATATTGGTGACCTGCGAATGTCTGATGGTTTGAACTATGAGAATAGAATTTCCGTGGGATTTCTGAATGACAATGTTGAGAATTCTATTGATAGCTACCGCGAAGCCTTTGACGTTGTTTACCTGAATGATGCACCCATGTGGGGAGTAGTAAAGCTTGTTTCACAACTATGTCCTGGAGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

337

Amino Acids

38.1

Weight (kDa)

5.91

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 78 - 332 7.7e-92 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 846
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 26, 95
AccII CGCG 1 cut(s) 927
AciI CCGC 3 cut(s) 191, 322, 925
AcsI RAATTY 2 cut(s) 873, 907
AfaI GTAC 2 cut(s) 97, 245
AfiI CCNNNNNNNGG 2 cut(s) 453, 968
AgsI TTSAA 3 cut(s) 377, 509, 859
AjnI CCWGG 3 cut(s) 74, 207, 1000
AleI CACNNNNGTG 1 cut(s) 964
AluBI AGCT 6 cut(s) 81, 328, 365, 391, 921, 982
AluI AGCT 6 cut(s) 81, 328, 365, 391, 921, 982
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 2 cut(s) 528, 637
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 3 cut(s) 280, 439, 767
ApeKI GCWGC 4 cut(s) 48, 81, 176, 731
ApoI RAATTY 2 cut(s) 873, 907
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 194, 586, 767
AsuHPI GGTGA 4 cut(s) 140, 146, 767, 845
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 194, 586
AxyI CCTNAGG 1 cut(s) 590
BanI GGYRCC 2 cut(s) 26, 95
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 4 cut(s) 35, 68, 163, 718
BccI CCATC 4 cut(s) 446, 637, 758, 845
BciT130I CCWGG 3 cut(s) 76, 209, 1002
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 528, 637
BfuAI ACCTGC 1 cut(s) 846
BfuI GTATCC 1 cut(s) 157
BisI GCNGC 4 cut(s) 49, 82, 177, 732
BlsI GCNGC 4 cut(s) 50, 83, 178, 733
Bme1390I CCNGG 3 cut(s) 76, 209, 1002
Bme18I GGWCC 2 cut(s) 194, 586
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 194, 586, 767
BmiI GGNNCC 4 cut(s) 28, 97, 195, 196
BmrFI CCNGG 3 cut(s) 76, 209, 1002
BmsI GCATC 2 cut(s) 217, 946
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 191
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 879
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 968
Bse21I CCTNAGG 1 cut(s) 590
BseBI CCWGG 3 cut(s) 76, 209, 1002
BseDI CCNNGG 1 cut(s) 879
BseGI GGATG 3 cut(s) 54, 343, 648
BseLI CCNNNNNNNGG 2 cut(s) 453, 968
BseMII CTCAG 1 cut(s) 581
BseRI GAGGAG 2 cut(s) 5, 596
BseXI GCAGC 4 cut(s) 35, 68, 163, 718
Bsh1236I CGCG 1 cut(s) 927
BshFI GGCC 3 cut(s) 282, 441, 769
BshNI GGYRCC 2 cut(s) 26, 95
BsiHKAI GWGCWC 1 cut(s) 717
BsiHKCI CYCGRG 1 cut(s) 261
BslFI GGGAC 2 cut(s) 180, 279
BslI CCNNNNNNNGG 2 cut(s) 453, 968
BsmAI GTCTC 2 cut(s) 528, 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 180, 279
BsmI GAATGC 2 cut(s) 193, 787
BsnI GGCC 3 cut(s) 282, 441, 769
BsoBI CYCGRG 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 2 cut(s) 627, 823
BspACI CCGC 3 cut(s) 191, 322, 925
BspANI GGCC 3 cut(s) 282, 441, 769
BspCNI CTCAG 1 cut(s) 582
BspFNI CGCG 1 cut(s) 927
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 4 cut(s) 28, 97, 195, 196
BspMI ACCTGC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 2 cut(s) 26, 95
BssECI CCNNGG 1 cut(s) 879
BssMI GATC 2 cut(s) 627, 823
Bst2UI CCWGG 3 cut(s) 76, 209, 1002
Bst6I CTCTTC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 363, 717
BstDEI CTNAG 2 cut(s) 387, 590
BstDSI CCRYGG 1 cut(s) 879
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 3 cut(s) 54, 343, 648
BstFNI CGCG 1 cut(s) 927
BstKTI GATC 2 cut(s) 630, 826
BstMAI GTCTC 2 cut(s) 528, 637
BstMBI GATC 2 cut(s) 627, 823
BstMWI GCNNNNNNNGC 3 cut(s) 45, 279, 566
BstNI CCWGG 3 cut(s) 76, 209, 1002
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 3 cut(s) 74, 207, 1000
BstUI CGCG 1 cut(s) 927
BstV1I GCAGC 4 cut(s) 35, 68, 163, 718
BstV2I GAAGAC 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 590
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 3 cut(s) 282, 441, 769
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 3 cut(s) 54, 343, 648
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 2 cut(s) 363, 717
CaiI CAGNNNCTG 1 cut(s) 81
CciI TCATGA 1 cut(s) 742
Cfr13I GGNCC 3 cut(s) 194, 586, 767
CseI GACGC 1 cut(s) 774
Csp6I GTAC 2 cut(s) 96, 244
CviAII CATG 4 cut(s) 118, 278, 743, 964
CviQI GTAC 2 cut(s) 96, 244
DdeI CTNAG 2 cut(s) 387, 590
DpnI GATC 2 cut(s) 629, 825
DpnII GATC 2 cut(s) 627, 823
DraI TTTAAA 2 cut(s) 616, 679
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco147I AGGCCT 1 cut(s) 441
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 194, 586
Eco81I CCTNAGG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 833
EcoO109I RGGNCCY 2 cut(s) 194, 586
EcoO65I GGTNACC 1 cut(s) 833
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 3 cut(s) 74, 207, 1000
EcoRV GATATC 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 4 cut(s) 121, 281, 746, 967
FaqI GGGAC 2 cut(s) 180, 279
FatI CATG 4 cut(s) 117, 277, 742, 963
FauI CCCGC 2 cut(s) 184, 315
Fnu4HI GCNGC 4 cut(s) 49, 82, 177, 732
FokI GGATG 3 cut(s) 41, 330, 655
Fsp4HI GCNGC 4 cut(s) 49, 82, 177, 732
GluI GCNGC 4 cut(s) 49, 82, 177, 732
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 3 cut(s) 282, 441, 769
HgaI GACGC 1 cut(s) 774
Hin1II CATG 4 cut(s) 121, 281, 746, 967
HindIII AAGCTT 2 cut(s) 389, 980
HinfI GANTC 5 cut(s) 122, 298, 415, 476, 691
HphI GGTGA 4 cut(s) 140, 146, 767, 845
Hpy166II GTNNAC 2 cut(s) 604, 946
Hpy188I TCNGA 5 cut(s) 111, 127, 186, 850, 891
Hpy188III TCNNGA 5 cut(s) 302, 589, 722, 743, 793
Hpy8I GTNNAC 2 cut(s) 604, 946
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 3 cut(s) 289, 569, 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 279, 566
HpyF3I CTNAG 2 cut(s) 387, 590
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 4 cut(s) 121, 281, 746, 967
KflI GGGWCCC 1 cut(s) 194
KpnI GGTACC 1 cut(s) 99
Kzo9I GATC 2 cut(s) 627, 823
LmnI GCTCC 3 cut(s) 78, 325, 739
Lsp1109I GCAGC 4 cut(s) 35, 68, 163, 718
LweI GCATC 2 cut(s) 217, 946
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 1 cut(s) 833
MalI GATC 2 cut(s) 629, 825
MboI GATC 2 cut(s) 627, 823
MboII GAAGA 5 cut(s) 10, 637, 697, 806, 813
MfeI CAATTG 2 cut(s) 372, 483
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
MlyI GAGTC 1 cut(s) 409
MseI TTAA 2 cut(s) 615, 678
MslI CAYNNNNRTG 3 cut(s) 831, 964, 994
MspR9I CCNGG 3 cut(s) 76, 209, 1002
MunI CAATTG 2 cut(s) 372, 483
Mva1269I GAATGC 2 cut(s) 193, 787
MvaI CCWGG 3 cut(s) 76, 209, 1002
MvnI CGCG 1 cut(s) 927
MwoI GCNNNNNNNGC 3 cut(s) 45, 279, 566
NdeII GATC 2 cut(s) 627, 823
NlaIII CATG 4 cut(s) 121, 281, 746, 967
NlaIV GGNNCC 4 cut(s) 28, 97, 195, 196
NmuCI GTSAC 1 cut(s) 833
NspV TTCGAA 1 cut(s) 333
OliI CACNNNNGTG 1 cut(s) 964
PagI TCATGA 1 cut(s) 742
PceI AGGCCT 1 cut(s) 441
PctI GAATGC 2 cut(s) 193, 787
PfeI GAWTC 4 cut(s) 122, 298, 476, 691
PfoI TCCNGGA 2 cut(s) 74, 1000
PkrI GCNGC 4 cut(s) 50, 83, 178, 733
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PpuMI RGGWCCY 2 cut(s) 194, 586
Psp5II RGGWCCY 2 cut(s) 194, 586
Psp6I CCWGG 3 cut(s) 74, 207, 1000
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 74, 207, 1000
PspN4I GGNNCC 4 cut(s) 28, 97, 195, 196
PspPI GGNCC 3 cut(s) 194, 586, 767
PspPPI RGGWCCY 2 cut(s) 194, 586
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 2 cut(s) 97, 245
RsaNI GTAC 2 cut(s) 96, 244
RseI CAYNNNNRTG 3 cut(s) 831, 964, 994
SaqAI TTAA 2 cut(s) 615, 678
SatI GCNGC 4 cut(s) 49, 82, 177, 732
Sau3AI GATC 2 cut(s) 627, 823
Sau96I GGNCC 3 cut(s) 194, 586, 767
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 3 cut(s) 76, 209, 1002
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 2 cut(s) 217, 946
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 2 cut(s) 194, 586
SmiMI CAYNNNNRTG 3 cut(s) 831, 964, 994
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 3 cut(s) 191, 322, 925
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 3 cut(s) 74, 207, 1000
TaiI ACGT 1 cut(s) 942
TaqI TCGA 1 cut(s) 333
TasI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 4 cut(s) 122, 298, 476, 691
Tru1I TTAA 2 cut(s) 615, 678
Tru9I TTAA 2 cut(s) 615, 678
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 4 cut(s) 48, 81, 176, 731
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 3 cut(s) 43, 683, 731
TspGWI ACGGA 2 cut(s) 457, 868
VpaK11BI GGWCC 2 cut(s) 194, 586
XapI RAATTY 2 cut(s) 873, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.