Rh1AG122400

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002535
Physical Location & Seq
Forward (+)
22900389 .. 22904581
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG122400.1

Sequence Viewer

Length: 1152 bp
ATGAGTAATCGCCTCCTCTTCGTTGGCACCACCATTTGCTCTAATCGCTGCTTCATCCCACACCACTTCACTTCCAGGACCTGCTTTTGTAGTGGTACCTTGAGAAATCAGACTAACATGGAATCCGATGAGGTTGTGGTGGTGGCTCACCCTCACCTTTTGGATACCAAAATTGCTGCTATTCGGAATGCGGGCCCCCCCAAACTCCAGGTTATAGCAGATTTTGATGCCACACTCACAAAGTACAGAGTGAATGGTTCTCGGGGACAAAGCAGTCATGGCCTTTTGCAACAGGAGAATCCTGAATATAATAACAAAAGGCGGGAGCTATTCGAATACTATCATCCATTAGAGTTCTCGCCAGCTATTCCAATTGAACAAAAAACTAAGCTTATGGAAGAATGGTGGGGAAAGACTCATTCTCTTTTGATTGAGGGAGGCCTTACCTATGATGGGATAAGGCAATCCGTTGCTGATTCCACAATTGCTTTTAGGGAGGGTGTTGTTGAATTATTTGAGTTTTTAGAGAAAAGAGACATTCCTGTTCTTATATTTTCTGCAGGACTTGCAGATATCATAGAGGAGGTCCTGAGGCAGAAAGTTCACAGATTGTTTAAAAATGTGAAGATCGTCTCCAATAGGATGGTATTTGATGATAACAGCCACCTTGTATCGTTTAAAGGGAAGACGATTCATAGTCTAAATAAAAATGAGCACGCTCTTGATATGGCTGCTCCACTTCATGAGCAATTAGGTGATTTAGATGGGCCAACTTATGAGAATGCGTCAGTCAAGACAAGAAGAAATGTCATTCTTCTGGGCGATCATATTGGTGACCTGGGAATGTCTGATGGTTTGAACTATGAGAATAGAATTTCCGTGGGATTTCTGTTGTGGCACACACATAAGGTAAGTTTTACAGAGATTATATTTGATGTGGTCTGGGTGATGAACTTAACTACAAGTTCATGGAAGCTACTTTGTCACTTAGTCTGGCTGTCAAAAATCACACATGCAGCAGGCTTGGACAGATTTTCCCTGGATGAACAACAGTATCGACTATCAAAGGTGAAGAGCATGGTTGTGGTGCTCTCCAAGGATTCTATAATGATCTGTAATTGCTCATGGAAGCAAGAAAGGCTGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

383

Amino Acids

43.67

Weight (kDa)

6.99

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 78 - 317 2e-78 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 89
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 26, 95
AciI CCGC 2 cut(s) 191, 322
AcsI RAATTY 1 cut(s) 873
AfaI GTAC 2 cut(s) 97, 245
AfiI CCNNNNNNNGG 1 cut(s) 453
AgsI TTSAA 3 cut(s) 377, 509, 859
AjnI CCWGG 4 cut(s) 74, 207, 837, 1038
AluBI AGCT 5 cut(s) 328, 365, 391, 976, 1149
AluI AGCT 5 cut(s) 328, 365, 391, 976, 1149
Alw21I GWGCWC 2 cut(s) 717, 1092
Alw26I GTCTC 2 cut(s) 528, 637
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 4 cut(s) 193, 280, 439, 767
ApaI GGGCCC 1 cut(s) 197
ApeKI GCWGC 5 cut(s) 48, 176, 731, 1016, 1146
ApoI RAATTY 1 cut(s) 873
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 5 cut(s) 78, 193, 194, 586, 767
AsuHPI GGTGA 6 cut(s) 140, 146, 767, 845, 958, 1081
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 78, 586
AxyI CCTNAGG 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 197
BaeI ACNNNNGTAYC 2 cut(s) 1037, 1070
BanI GGYRCC 2 cut(s) 26, 95
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 2 cut(s) 717, 1092
BbvI GCAGC 4 cut(s) 35, 163, 718, 1028
BccI CCATC 4 cut(s) 446, 637, 758, 845
BciT130I CCWGG 4 cut(s) 76, 209, 839, 1040
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 528, 637
BfaI CTAG 1 cut(s) 1150
BfmI CTRYAG 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 157
BisI GCNGC 5 cut(s) 49, 177, 732, 1017, 1147
BlpI GCTNAGC 1 cut(s) 1142
BlsI GCNGC 5 cut(s) 50, 178, 733, 1018, 1148
Bme1390I CCNGG 4 cut(s) 76, 209, 839, 1040
Bme18I GGWCC 2 cut(s) 78, 586
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 5 cut(s) 78, 193, 194, 586, 767
BmiI GGNNCC 4 cut(s) 28, 97, 195, 196
BmrFI CCNGG 4 cut(s) 76, 209, 839, 1040
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 1142
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 121
BsaJI CCNNGG 4 cut(s) 838, 879, 1038, 1095
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse21I CCTNAGG 1 cut(s) 590
BseBI CCWGG 4 cut(s) 76, 209, 839, 1040
BseDI CCNNGG 4 cut(s) 838, 879, 1038, 1095
BseGI GGATG 4 cut(s) 54, 343, 648, 1048
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 581, 1133
BseRI GAGGAG 2 cut(s) 5, 596
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 4 cut(s) 35, 163, 718, 1028
BshFI GGCC 4 cut(s) 195, 282, 441, 769
BshNI GGYRCC 2 cut(s) 26, 95
BsiHKAI GWGCWC 2 cut(s) 717, 1092
BsiHKCI CYCGRG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 2 cut(s) 528, 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 2 cut(s) 193, 787
BsnI GGCC 4 cut(s) 195, 282, 441, 769
BsoBI CYCGRG 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 333
Bsp120I GGGCCC 1 cut(s) 193
Bsp1286I GDGCHC 3 cut(s) 197, 717, 1092
Bsp143I GATC 3 cut(s) 627, 823, 1110
Bsp1720I GCTNAGC 1 cut(s) 1142
BspACI CCGC 2 cut(s) 191, 322
BspANI GGCC 4 cut(s) 195, 282, 441, 769
BspCNI CTCAG 2 cut(s) 582, 1134
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 4 cut(s) 28, 97, 195, 196
BspMAI CTGCAG 1 cut(s) 562
BspMI ACCTGC 1 cut(s) 89
BspQI GCTCTTC 1 cut(s) 1067
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 2 cut(s) 26, 95
BssECI CCNNGG 4 cut(s) 838, 879, 1038, 1095
BssMI GATC 3 cut(s) 627, 823, 1110
BssT1I CCWWGG 1 cut(s) 1095
Bst2UI CCWGG 4 cut(s) 76, 209, 839, 1040
Bst4CI ACNGT 1 cut(s) 1053
Bst6I CTCTTC 2 cut(s) 23, 1067
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 4 cut(s) 193, 363, 717, 1021
BstDEI CTNAG 4 cut(s) 387, 590, 988, 1142
BstDSI CCRYGG 1 cut(s) 879
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 4 cut(s) 54, 343, 648, 1048
BstKTI GATC 3 cut(s) 630, 826, 1113
BstMAI GTCTC 2 cut(s) 528, 637
BstMBI GATC 3 cut(s) 627, 823, 1110
BstMWI GCNNNNNNNGC 4 cut(s) 45, 279, 566, 1138
BstNI CCWGG 4 cut(s) 76, 209, 839, 1040
BstNSI RCATGY 1 cut(s) 1016
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 4 cut(s) 74, 207, 837, 1038
BstSFI CTRYAG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 197
BstV1I GCAGC 4 cut(s) 35, 163, 718, 1028
BstV2I GAAGAC 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 590
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 4 cut(s) 195, 282, 441, 769
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 4 cut(s) 54, 343, 648, 1048
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 4 cut(s) 193, 363, 717, 1021
CaiI CAGNNNCTG 1 cut(s) 81
CciI TCATGA 1 cut(s) 742
Cfr13I GGNCC 5 cut(s) 78, 193, 194, 586, 767
CseI GACGC 1 cut(s) 774
Csp6I GTAC 2 cut(s) 96, 244
CviAII CATG 7 cut(s) 118, 278, 743, 969, 1013, 1078, 1125
CviQI GTAC 2 cut(s) 96, 244
DdeI CTNAG 4 cut(s) 387, 590, 988, 1142
DpnI GATC 3 cut(s) 629, 825, 1112
DpnII GATC 3 cut(s) 627, 823, 1110
DraI TTTAAA 2 cut(s) 616, 679
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 23, 1067
EarI CTCTTC 2 cut(s) 23, 1067
Eco130I CCWWGG 1 cut(s) 1095
Eco147I AGGCCT 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 197
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 78, 586
Eco81I CCTNAGG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 833
EcoO109I RGGNCCY 3 cut(s) 78, 194, 586
EcoO65I GGTNACC 1 cut(s) 833
EcoRII CCWGG 4 cut(s) 74, 207, 837, 1038
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 1095
EcoT38I GRGCYC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 1095
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 7 cut(s) 121, 281, 746, 972, 1016, 1081, 1128
FaqI GGGAC 1 cut(s) 279
FatI CATG 7 cut(s) 117, 277, 742, 968, 1012, 1077, 1124
FauI CCCGC 2 cut(s) 184, 315
Fnu4HI GCNGC 5 cut(s) 49, 177, 732, 1017, 1147
FokI GGATG 4 cut(s) 41, 330, 655, 1055
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 5 cut(s) 49, 177, 732, 1017, 1147
FspBI CTAG 1 cut(s) 1150
GluI GCNGC 5 cut(s) 49, 177, 732, 1017, 1147
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 4 cut(s) 195, 282, 441, 769
HgaI GACGC 1 cut(s) 774
Hin1II CATG 7 cut(s) 121, 281, 746, 972, 1016, 1081, 1128
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 6 cut(s) 122, 298, 415, 476, 691, 1100
HphI GGTGA 6 cut(s) 140, 146, 767, 845, 958, 1081
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 4 cut(s) 111, 127, 186, 850
Hpy188III TCNNGA 5 cut(s) 302, 589, 722, 743, 793
Hpy8I GTNNAC 1 cut(s) 604
HpyCH4III ACNGT 1 cut(s) 1053
HpyCH4V TGCA 4 cut(s) 289, 560, 569, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 279, 566, 1138
HpyF3I CTNAG 4 cut(s) 387, 590, 988, 1142
Hsp92II CATG 7 cut(s) 121, 281, 746, 972, 1016, 1081, 1128
KpnI GGTACC 1 cut(s) 99
Kzo9I GATC 3 cut(s) 627, 823, 1110
LguI GCTCTTC 1 cut(s) 1067
LmnI GCTCC 2 cut(s) 325, 739
Lsp1109I GCAGC 4 cut(s) 35, 163, 718, 1028
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 1150
MaeIII GTNAC 2 cut(s) 833, 983
MalI GATC 3 cut(s) 629, 825, 1112
MboI GATC 3 cut(s) 627, 823, 1110
MboII GAAGA 7 cut(s) 10, 410, 637, 697, 806, 813, 1084
MfeI CAATTG 2 cut(s) 372, 483
MhlI GDGCHC 3 cut(s) 197, 717, 1092
MluCI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 1117
MlyI GAGTC 1 cut(s) 409
MseI TTAA 3 cut(s) 615, 678, 956
MslI CAYNNNNRTG 2 cut(s) 831, 1082
MspR9I CCNGG 4 cut(s) 76, 209, 839, 1040
MunI CAATTG 2 cut(s) 372, 483
Mva1269I GAATGC 2 cut(s) 193, 787
MvaI CCWGG 4 cut(s) 76, 209, 839, 1040
MwoI GCNNNNNNNGC 4 cut(s) 45, 279, 566, 1138
NdeII GATC 3 cut(s) 627, 823, 1110
NlaIII CATG 7 cut(s) 121, 281, 746, 972, 1016, 1081, 1128
NlaIV GGNNCC 4 cut(s) 28, 97, 195, 196
NmuCI GTSAC 2 cut(s) 833, 983
NspI RCATGY 1 cut(s) 1016
NspV TTCGAA 1 cut(s) 333
PagI TCATGA 1 cut(s) 742
PceI AGGCCT 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 1067
PctI GAATGC 2 cut(s) 193, 787
PfeI GAWTC 5 cut(s) 122, 298, 476, 691, 1100
PfoI TCCNGGA 1 cut(s) 74
PkrI GCNGC 5 cut(s) 50, 178, 733, 1018, 1148
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PpuMI RGGWCCY 2 cut(s) 78, 586
Psp5II RGGWCCY 2 cut(s) 78, 586
Psp6I CCWGG 4 cut(s) 74, 207, 837, 1038
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 4 cut(s) 74, 207, 837, 1038
PspN4I GGNNCC 4 cut(s) 28, 97, 195, 196
PspOMI GGGCCC 1 cut(s) 193
PspPI GGNCC 5 cut(s) 78, 193, 194, 586, 767
PspPPI RGGWCCY 2 cut(s) 78, 586
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 2 cut(s) 97, 245
RsaNI GTAC 2 cut(s) 96, 244
RseI CAYNNNNRTG 2 cut(s) 831, 1082
SapI GCTCTTC 1 cut(s) 1067
SaqAI TTAA 3 cut(s) 615, 678, 956
SatI GCNGC 5 cut(s) 49, 177, 732, 1017, 1147
Sau3AI GATC 3 cut(s) 627, 823, 1110
Sau96I GGNCC 5 cut(s) 78, 193, 194, 586, 767
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 4 cut(s) 76, 209, 839, 1040
SduI GDGCHC 3 cut(s) 197, 717, 1092
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 2 cut(s) 78, 586
SmiMI CAYNNNNRTG 2 cut(s) 831, 1082
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 1117
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 2 cut(s) 191, 322
SspMI CTAG 1 cut(s) 1150
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 4 cut(s) 74, 207, 837, 1038
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 1 cut(s) 1053
TaqI TCGA 2 cut(s) 333, 1057
TasI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 1117
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 5 cut(s) 122, 298, 476, 691, 1100
Tru1I TTAA 3 cut(s) 615, 678, 956
Tru9I TTAA 3 cut(s) 615, 678, 956
TseFI GTSAC 2 cut(s) 833, 983
TseI GCWGC 5 cut(s) 48, 176, 731, 1016, 1146
Tsp45I GTSAC 2 cut(s) 833, 983
TspDTI ATGAA 6 cut(s) 43, 683, 731, 957, 965, 1059
TspGWI ACGGA 2 cut(s) 457, 868
VpaK11BI GGWCC 2 cut(s) 78, 586
XapI RAATTY 1 cut(s) 873
XceI RCATGY 1 cut(s) 1016
XspI CTAG 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.