MD07G1062700.v1.1

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002535
Physical Location & Seq
Forward (+)
5883892 .. 5887920
4029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1062700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGAGCATTAATCGTCTCCAAAGCATCCTCCTTCTCGGCACCACCCCCCCCCACCGCCGCTTGCTTCCACCCCACTCGTTTATTTCCAGGGCTTGGTTTTGTAGCGGCAGAAGCTTCAGAAATCCGATCCACATGGAAACCGATGCTTCTTGTGACGCGGCGGTTGTGGGTGACTCCGACCTCCTGCTCAAGAAAATTGATGCTATTCGCGATGCCGGTCCCGCCAAACTTCAGGTAATAGCAGATTTTGATGCCACCTTAACCAGGTACCGGGTCAATGGTTGCCGGGGACAAAGTAGTCATGGCCTTTTGCAGCAGGAGAATCCGGAATATGATAGGAAAAGACAGGAATTGTATGAACATTATCATCCGTTAGAGTTCTCCCCGACAATTCCACTTGAAGAGAAAACTAAGCTTATGGAAGAATGGTGGGGGAGAACTCATGGTCTCTTAGTTGAGGGTGGCGTTACATATGATGGAATAAAGCAATCTGTTGCTGACTCCACGATTGCTTTTAGGGAGGGTGTTGTAGAACTATTTGAGTTTTTGGAGGAAAGAGACATCCCTGTTCTTATATTTTCTGCGGGACTTGCTGATATCATAGAGGAGGTCATGCGGCAGAAGGTTCACAGACTTTTCAAAAATGTGAAGATTGTTTCCAATCGGATGGAATTTGATGATAACGGTCGCCTCTTATCTTTCAGAGGGAAGACGATTCATAGTTTGAATAAAAATGAGCATGCTCTTGATATGGCCGGTCCCCTTCATGATCGATTAGGTGATGTAGACGGGCCACCTTATGAGAATGCCTCAGTCAAGACCAGGTTAAATGTCCTGCTTCTGGGTGATCACATTGGTGACCTTGGAATGTCTGACGGTTTGAACTATGAGAATAGAATATCCGTGGGATTTCTGAATGACAATGTGGAGAACTCTCTGGATGGCTATCGAAAAGCCTTTGATATGGTTTACCTGAATGATGCACCCATGTGGGGAGTCGTAAAGCTTGTTTCGCAACTTTGTCCAGTTGGAGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

347

Amino Acids

38.99

Weight (kDa)

5.58

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 86 - 340 9.1e-91 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 297
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 2 cut(s) 38, 267
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 1 cut(s) 786
AccII CGCG 2 cut(s) 158, 210
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 8 cut(s) 55, 58, 105, 158, 161, 222, 584, 616
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 2 cut(s) 100, 215
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 6 cut(s) 93, 264, 270, 841, 992, 1031
AgsI TTSAA 4 cut(s) 401, 640, 727, 883
AjnI CCWGG 3 cut(s) 86, 263, 821
AleI CACNNNNGTG 2 cut(s) 855, 988
AluBI AGCT 3 cut(s) 114, 415, 1006
AluI AGCT 3 cut(s) 114, 415, 1006
Alw26I GTCTC 3 cut(s) 20, 452, 552
AlwI GGATC 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 3 cut(s) 304, 753, 791
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 1 cut(s) 671
AseI ATTAAT 1 cut(s) 9
Asp718I GGTACC 1 cut(s) 267
AspS9I GGNCC 3 cut(s) 218, 758, 791
AsuC2I CCSGG 2 cut(s) 272, 287
AsuHPI GGTGA 4 cut(s) 182, 791, 857, 869
AvaII GGWCC 2 cut(s) 218, 758
BanI GGYRCC 2 cut(s) 38, 267
BbsI GAAGAC 1 cut(s) 716
BbvI GCAGC 1 cut(s) 325
BccI CCATC 3 cut(s) 470, 661, 935
BciT130I CCWGG 3 cut(s) 88, 265, 823
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 2 cut(s) 272, 287
BcoDI GTCTC 3 cut(s) 20, 452, 552
BisI GCNGC 5 cut(s) 58, 106, 159, 314, 617
BlsI GCNGC 5 cut(s) 59, 107, 160, 315, 618
Bme1390I CCNGG 5 cut(s) 88, 265, 272, 287, 823
Bme18I GGWCC 2 cut(s) 218, 758
BmgT120I GGNCC 3 cut(s) 218, 758, 791
BmiI GGNNCC 4 cut(s) 40, 220, 269, 760
BmrFI CCNGG 5 cut(s) 88, 265, 272, 287, 823
BmsI GCATC 6 cut(s) 33, 133, 190, 202, 241, 970
BpiI GAAGAC 1 cut(s) 716
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 272, 287
Bsa29I ATCGAT 1 cut(s) 772
BsaBI GATNNNNATC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 87, 286, 862, 903
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 264, 270, 841, 992, 1031
Bse118I RCCGGY 2 cut(s) 215, 755
Bse1I ACTGG 1 cut(s) 1025
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 3 cut(s) 88, 265, 823
BseCI ATCGAT 1 cut(s) 772
BseDI CCNNGG 4 cut(s) 87, 286, 862, 903
BseGI GGATG 5 cut(s) 24, 367, 561, 672, 946
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 6 cut(s) 93, 264, 270, 841, 992, 1031
BseMII CTCAG 1 cut(s) 825
BseNI ACTGG 1 cut(s) 1025
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 1 cut(s) 325
Bsh1236I CGCG 2 cut(s) 158, 210
Bsh1285I CGRYCG 1 cut(s) 688
BshFI GGCC 3 cut(s) 306, 755, 793
BshNI GGYRCC 2 cut(s) 38, 267
BshVI ATCGAT 1 cut(s) 772
BsiEI CGRYCG 1 cut(s) 688
BsiSI CCGG 5 cut(s) 216, 271, 286, 326, 756
BslFI GGGAC 4 cut(s) 204, 303, 600, 744
BslI CCNNNNNNNGG 6 cut(s) 93, 264, 270, 841, 992, 1031
BsmAI GTCTC 3 cut(s) 20, 452, 552
BsmBI CGTCTC 1 cut(s) 20
BsmFI GGGAC 4 cut(s) 204, 303, 600, 744
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 3 cut(s) 306, 755, 793
Bso31I GGTCTC 1 cut(s) 452
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 3 cut(s) 126, 769, 847
Bsp68I TCGCGA 1 cut(s) 210
BspACI CCGC 8 cut(s) 55, 58, 105, 158, 161, 222, 584, 616
BspANI GGCC 3 cut(s) 306, 755, 793
BspCNI CTCAG 1 cut(s) 824
BspDI ATCGAT 1 cut(s) 772
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 2 cut(s) 158, 210
BspHI TCATGA 1 cut(s) 766
BspLI GGNNCC 4 cut(s) 40, 220, 269, 760
BspPI GGATC 1 cut(s) 121
BspT107I GGYRCC 2 cut(s) 38, 267
BspTNI GGTCTC 1 cut(s) 452
BsrFI RCCGGY 2 cut(s) 215, 755
BsrI ACTGG 1 cut(s) 1025
BssAI RCCGGY 2 cut(s) 215, 755
BssECI CCNNGG 4 cut(s) 87, 286, 862, 903
BssMI GATC 3 cut(s) 126, 769, 847
BssT1I CCWWGG 1 cut(s) 862
Bst2UI CCWGG 3 cut(s) 88, 265, 823
Bst4CI ACNGT 2 cut(s) 686, 878
Bst6I CTCTTC 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 62, 741
BstDEI CTNAG 3 cut(s) 411, 451, 811
BstDSI CCRYGG 1 cut(s) 903
BstEII GGTNACC 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 262
BstF5I GGATG 5 cut(s) 24, 367, 561, 672, 946
BstFNI CGCG 2 cut(s) 158, 210
BstKTI GATC 3 cut(s) 129, 772, 850
BstMAI GTCTC 3 cut(s) 20, 452, 552
BstMBI GATC 3 cut(s) 126, 769, 847
BstMCI CGRYCG 1 cut(s) 688
BstMWI GCNNNNNNNGC 4 cut(s) 111, 221, 590, 1012
BstNI CCWGG 3 cut(s) 88, 265, 823
BstNSI RCATGY 1 cut(s) 743
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 5 cut(s) 86, 263, 270, 285, 821
BstUI CGCG 2 cut(s) 158, 210
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 716
BstXI CCANNNNNNTGG 1 cut(s) 667
Bsu15I ATCGAT 1 cut(s) 772
BsuRI GGCC 3 cut(s) 306, 755, 793
BsuTUI ATCGAT 1 cut(s) 772
BtgI CCRYGG 1 cut(s) 903
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 5 cut(s) 24, 367, 561, 672, 946
BtuMI TCGCGA 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 62, 741
CciI TCATGA 1 cut(s) 766
Cfr10I RCCGGY 2 cut(s) 215, 755
Cfr13I GGNCC 3 cut(s) 218, 758, 791
ClaI ATCGAT 1 cut(s) 772
CseI GACGC 1 cut(s) 164
CsiI ACCWGGT 2 cut(s) 263, 821
Csp6I GTAC 1 cut(s) 268
CviAII CATG 7 cut(s) 133, 302, 443, 613, 740, 767, 988
CviJI RGCY 9 cut(s) 92, 114, 306, 415, 755, 793, 945, 956, 1006
CviKI_1 RGCY 9 cut(s) 92, 114, 306, 415, 755, 793, 945, 956, 1006
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 411, 451, 811
DpnI GATC 3 cut(s) 128, 771, 849
DpnII GATC 3 cut(s) 126, 769, 847
DrdI GACNNNNNNGTC 1 cut(s) 297
DseDI GACNNNNNNGTC 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 753
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 862
Eco31I GGTCTC 1 cut(s) 452
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 218, 758
Eco57I CTGAAG 2 cut(s) 100, 215
Eco91I GGTNACC 1 cut(s) 857
EcoNI CCTNNNNNAGG 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 857
EcoRII CCWGG 3 cut(s) 86, 263, 821
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 862
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 7 cut(s) 136, 305, 446, 616, 743, 770, 991
FaqI GGGAC 4 cut(s) 204, 303, 600, 744
FatI CATG 7 cut(s) 132, 301, 442, 612, 739, 766, 987
FauI CCCGC 2 cut(s) 229, 577
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 58, 106, 159, 314, 617
FokI GGATG 5 cut(s) 11, 354, 548, 679, 953
Fsp4HI GCNGC 5 cut(s) 58, 106, 159, 314, 617
GluI GCNGC 5 cut(s) 58, 106, 159, 314, 617
HaeIII GGCC 3 cut(s) 306, 755, 793
HapII CCGG 5 cut(s) 216, 271, 286, 326, 756
HgaI GACGC 1 cut(s) 164
Hin1II CATG 7 cut(s) 136, 305, 446, 616, 743, 770, 991
HindIII AAGCTT 3 cut(s) 112, 413, 1004
HinfI GANTC 5 cut(s) 173, 322, 500, 715, 996
HpaII CCGG 5 cut(s) 216, 271, 286, 326, 756
HphI GGTGA 4 cut(s) 182, 791, 857, 869
Hpy166II GTNNAC 3 cut(s) 628, 787, 970
Hpy188I TCNGA 7 cut(s) 119, 126, 178, 666, 704, 874, 915
Hpy188III TCNNGA 7 cut(s) 190, 209, 326, 746, 767, 817, 938
Hpy8I GTNNAC 3 cut(s) 628, 787, 970
HpyAV CCTTC 3 cut(s) 41, 616, 773
HpyCH4III ACNGT 2 cut(s) 686, 878
HpyCH4V TGCA 2 cut(s) 313, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 221, 590, 1012
HpyF3I CTNAG 3 cut(s) 411, 451, 811
Hsp92II CATG 7 cut(s) 136, 305, 446, 616, 743, 770, 991
Kpn2I TCCGGA 1 cut(s) 325
KpnI GGTACC 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 3 cut(s) 126, 769, 847
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 6 cut(s) 33, 133, 190, 202, 241, 970
MabI ACCWGGT 2 cut(s) 263, 821
MaeIII GTNAC 4 cut(s) 152, 170, 466, 857
MalI GATC 3 cut(s) 128, 771, 849
MboI GATC 3 cut(s) 126, 769, 847
MboII GAAGA 4 cut(s) 413, 434, 661, 721
MluCI AATT 4 cut(s) 195, 350, 390, 671
MlyI GAGTC 3 cut(s) 167, 494, 1005
MmeI TCCRAC 2 cut(s) 201, 1009
MroI TCCGGA 1 cut(s) 325
MseI TTAA 3 cut(s) 9, 260, 827
MslI CAYNNNNRTG 2 cut(s) 855, 988
MspI CCGG 5 cut(s) 216, 271, 286, 326, 756
MspR9I CCNGG 5 cut(s) 88, 265, 272, 287, 823
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 3 cut(s) 88, 265, 823
MvnI CGCG 2 cut(s) 158, 210
MwoI GCNNNNNNNGC 4 cut(s) 111, 221, 590, 1012
NciI CCSGG 2 cut(s) 272, 287
NdeI CATATG 1 cut(s) 472
NdeII GATC 3 cut(s) 126, 769, 847
NlaIII CATG 7 cut(s) 136, 305, 446, 616, 743, 770, 991
NlaIV GGNNCC 4 cut(s) 40, 220, 269, 760
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 3 cut(s) 152, 170, 857
NruI TCGCGA 1 cut(s) 210
NspI RCATGY 1 cut(s) 743
OliI CACNNNNGTG 2 cut(s) 855, 988
PaeI GCATGC 1 cut(s) 743
PagI TCATGA 1 cut(s) 766
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 2 cut(s) 322, 715
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 5 cut(s) 59, 107, 160, 315, 618
PleI GAGTC 3 cut(s) 167, 494, 1004
PpsI GAGTC 3 cut(s) 167, 494, 1004
PshBI ATTAAT 1 cut(s) 9
Psp6I CCWGG 3 cut(s) 86, 263, 821
PspEI GGTNACC 1 cut(s) 857
PspGI CCWGG 3 cut(s) 86, 263, 821
PspN4I GGNNCC 4 cut(s) 40, 220, 269, 760
PspPI GGNCC 3 cut(s) 218, 758, 791
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 2 cut(s) 855, 988
SaqAI TTAA 3 cut(s) 9, 260, 827
SatI GCNGC 5 cut(s) 58, 106, 159, 314, 617
Sau3AI GATC 3 cut(s) 126, 769, 847
Sau96I GGNCC 3 cut(s) 218, 758, 791
SchI GAGTC 3 cut(s) 167, 494, 1005
ScrFI CCNGG 5 cut(s) 88, 265, 272, 287, 823
SexAI ACCWGGT 2 cut(s) 263, 821
SfaNI GCATC 6 cut(s) 33, 133, 190, 202, 241, 970
SinI GGWCC 2 cut(s) 218, 758
SmiMI CAYNNNNRTG 2 cut(s) 855, 988
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SphI GCATGC 1 cut(s) 743
Sse9I AATT 4 cut(s) 195, 350, 390, 671
SsiI CCGC 8 cut(s) 55, 58, 105, 158, 161, 222, 584, 616
StyD4I CCNGG 5 cut(s) 86, 263, 270, 285, 821
StyI CCWWGG 1 cut(s) 862
TaaI ACNGT 2 cut(s) 686, 878
TaqI TCGA 2 cut(s) 772, 949
TasI AATT 4 cut(s) 195, 350, 390, 671
TauI GCSGC 4 cut(s) 60, 108, 161, 619
TfiI GAWTC 2 cut(s) 322, 715
Tru1I TTAA 3 cut(s) 9, 260, 827
Tru9I TTAA 3 cut(s) 9, 260, 827
TseFI GTSAC 3 cut(s) 152, 170, 857
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 3 cut(s) 152, 170, 857
TspDTI ATGAA 3 cut(s) 372, 707, 755
TspGWI ACGGA 2 cut(s) 360, 892
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 2 cut(s) 218, 758
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 262
XapI RAATTY 1 cut(s) 671
XceI RCATGY 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.