FvH4_7g05460

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002535
Physical Location & Seq
Forward (+)
5754857 .. 5760961
6105 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05460.t2

Sequence Viewer

Length: 738 bp
ATGGCTGTCGGGGACAAAGAGAATCCTGAATATGATAACAAGAGGCGTCAGTTATACGAATACTATCATCCGTTAGAGTTTTCCCCAACTATTCCAATTCAGCAGAAAACTCTGCTTATGGAAGAATGGTGGGGAAAAACTCATTCTCTTTTGGTTGAAGGAGGCGTTACGTATGATGGGATAAAGCAATCTGTTGCTGATTCAGCAATCTCTTTTAGGGAGGGTGTTGTTGAATTATTTGAGTTTTTAGAGAAAAGAGACATTCCCGTTCTTATATTTTCTGCAGGACTTGCGGATATCATTGAGGAGGTCCTGAGGCAGAAAGTTCACAGATTATTCAAAAATGTGAAGATCGTCTCCAACAGGATGGTCTTTGATGAAAACGGCCGCCTTGTATCTTTTAAAGGGAAGACGATACATAGTCTGAATAAAAATGAGCATGCTCTTGATATGGCTGCTCCACTTCATGAGCAATTAGGTGATTCAGACGGACCAACTTATGAGAATGTGTTAGTCAAGACAAGAAGAAATATTATTCTTTTGGGTGATCATATTGGTGACCTGGGGATGTCTGATGGTTTGAACTATGAGAATAGAATTTCCGTGGGATTTCTGAATGACAATGTTAAGAATTCCATTGATAGTTACAGCAAAGCCTTTGATGTTGTTTACCTGAATGATGCACCCATGTGGGGAGTAGTAAAGCTTGTTTCAGAACTATGTCCTGGAGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

246

Amino Acids

27.79

Weight (kDa)

5.14

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 72 - 326 2.2e-90 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 293, 388
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 2 cut(s) 597, 631
AcyI GRCGYC 1 cut(s) 46
AfiI CCNNNNNNNGG 1 cut(s) 692
AgsI TTSAA 4 cut(s) 158, 233, 340, 583
AjnI CCWGG 2 cut(s) 561, 724
AleI CACNNNNGTG 1 cut(s) 688
AluBI AGCT 1 cut(s) 706
AluI AGCT 1 cut(s) 706
Alw26I GTCTC 2 cut(s) 252, 361
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 2 cut(s) 597, 631
AspS9I GGNCC 2 cut(s) 310, 491
AsuHPI GGTGA 3 cut(s) 491, 557, 569
AvaII GGWCC 2 cut(s) 310, 491
AxyI CCTNAGG 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 442
BccI CCATC 3 cut(s) 170, 361, 569
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 2 cut(s) 563, 726
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 252, 361
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 2 cut(s) 388, 456
BlsI GCNGC 2 cut(s) 389, 457
Bme1390I CCNGG 2 cut(s) 563, 726
Bme18I GGWCC 2 cut(s) 310, 491
BmgT120I GGNCC 2 cut(s) 310, 491
BmrFI CCNGG 2 cut(s) 563, 726
BmsI GCATC 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 562, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 692
Bse21I CCTNAGG 1 cut(s) 314
BseBI CCWGG 2 cut(s) 563, 726
BseDI CCNNGG 2 cut(s) 562, 603
BseGI GGATG 3 cut(s) 67, 372, 573
BseLI CCNNNNNNNGG 1 cut(s) 692
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 320
BseX3I CGGCCG 1 cut(s) 385
BseXI GCAGC 1 cut(s) 442
Bsh1285I CGRYCG 1 cut(s) 388
BshFI GGCC 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 692
BsmAI GTCTC 2 cut(s) 252, 361
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 351, 547
BspACI CCGC 2 cut(s) 293, 388
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 562, 603
BssMI GATC 2 cut(s) 351, 547
BssNI GRCGYC 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 563, 726
BstACI GRCGYC 1 cut(s) 46
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 603
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 3 cut(s) 67, 372, 573
BstKTI GATC 2 cut(s) 354, 550
BstMAI GTCTC 2 cut(s) 252, 361
BstMBI GATC 2 cut(s) 351, 547
BstMCI CGRYCG 1 cut(s) 388
BstMWI GCNNNNNNNGC 2 cut(s) 203, 290
BstNI CCWGG 2 cut(s) 563, 726
BstNSI RCATGY 1 cut(s) 443
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 561, 724
BstSFI CTRYAG 1 cut(s) 282
BstSNI TACGTA 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 442
BstV2I GAAGAC 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 367
BstZI CGGCCG 1 cut(s) 385
Bsu36I CCTNAGG 1 cut(s) 314
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 3 cut(s) 67, 372, 573
Cac8I GCNNGC 1 cut(s) 441
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 2 cut(s) 310, 491
CseI GACGC 1 cut(s) 35
CviAII CATG 3 cut(s) 440, 467, 688
CviJI RGCY 5 cut(s) 5, 387, 455, 656, 706
CviKI_1 RGCY 5 cut(s) 5, 387, 455, 656, 706
DdeI CTNAG 1 cut(s) 314
DpnI GATC 2 cut(s) 353, 549
DpnII GATC 2 cut(s) 351, 547
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 385
EagI CGGCCG 1 cut(s) 385
EclXI CGGCCG 1 cut(s) 385
Eco105I TACGTA 1 cut(s) 171
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 310, 491
Eco52I CGGCCG 1 cut(s) 385
Eco81I CCTNAGG 1 cut(s) 314
Eco91I GGTNACC 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 561, 724
EcoRV GATATC 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 3 cut(s) 443, 470, 691
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 439, 466, 687
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 388, 456
FokI GGATG 3 cut(s) 54, 379, 580
Fsp4HI GCNGC 2 cut(s) 388, 456
GluI GCNGC 2 cut(s) 388, 456
HaeIII GGCC 1 cut(s) 387
HgaI GACGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 3 cut(s) 443, 470, 691
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 22, 200, 482
HphI GGTGA 3 cut(s) 491, 557, 569
Hpy166II GTNNAC 2 cut(s) 328, 670
Hpy188I TCNGA 5 cut(s) 426, 487, 574, 615, 715
Hpy188III TCNNGA 5 cut(s) 26, 313, 446, 467, 517
Hpy8I GTNNAC 2 cut(s) 328, 670
HpyAV CCTTC 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 2 cut(s) 284, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 290
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 3 cut(s) 443, 470, 691
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 2 cut(s) 351, 547
LmnI GCTCC 1 cut(s) 463
LpnPI CCDG 8 cut(s) 39, 270, 326, 349, 548, 575, 686, 711
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 1 cut(s) 670
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 166, 557, 644
MalI GATC 2 cut(s) 353, 549
MboI GATC 2 cut(s) 351, 547
MboII GAAGA 4 cut(s) 134, 361, 421, 537
MluCI AATT 5 cut(s) 96, 233, 473, 597, 631
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 6 cut(s) 36, 155, 214, 298, 301, 309
MseI TTAA 2 cut(s) 402, 627
MslI CAYNNNNRTG 2 cut(s) 555, 688
MspR9I CCNGG 2 cut(s) 563, 726
MvaI CCWGG 2 cut(s) 563, 726
MwoI GCNNNNNNNGC 2 cut(s) 203, 290
NdeII GATC 2 cut(s) 351, 547
NlaIII CATG 3 cut(s) 443, 470, 691
NmuCI GTSAC 1 cut(s) 557
NspI RCATGY 1 cut(s) 443
OliI CACNNNNGTG 1 cut(s) 688
PaeI GCATGC 1 cut(s) 443
PagI TCATGA 1 cut(s) 466
PfeI GAWTC 3 cut(s) 22, 200, 482
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 2 cut(s) 389, 457
Ppu21I YACGTR 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 561, 724
PspEI GGTNACC 1 cut(s) 557
PspGI CCWGG 2 cut(s) 561, 724
PspPI GGNCC 2 cut(s) 310, 491
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 286
RseI CAYNNNNRTG 2 cut(s) 555, 688
SaqAI TTAA 2 cut(s) 402, 627
SatI GCNGC 2 cut(s) 388, 456
Sau3AI GATC 2 cut(s) 351, 547
Sau96I GGNCC 2 cut(s) 310, 491
ScrFI CCNGG 2 cut(s) 563, 726
SetI ASST 6 cut(s) 173, 312, 481, 564, 675, 708
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 1 cut(s) 282
SinI GGWCC 2 cut(s) 310, 491
SmiMI CAYNNNNRTG 2 cut(s) 555, 688
SnaBI TACGTA 1 cut(s) 171
SphI GCATGC 1 cut(s) 443
Sse9I AATT 5 cut(s) 96, 233, 473, 597, 631
SsiI CCGC 2 cut(s) 293, 388
SspI AATATT 1 cut(s) 532
StyD4I CCNGG 2 cut(s) 561, 724
TaiI ACGT 1 cut(s) 173
TasI AATT 5 cut(s) 96, 233, 473, 597, 631
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 3 cut(s) 22, 200, 482
Tru1I TTAA 2 cut(s) 402, 627
Tru9I TTAA 2 cut(s) 402, 627
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 393, 455
TspGWI ACGGA 3 cut(s) 60, 504, 592
VpaK11BI GGWCC 2 cut(s) 310, 491
XapI RAATTY 2 cut(s) 597, 631
XceI RCATGY 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.