FvH4_1g28480

CS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011066
Physical Location & Seq
Reverse (-)
20537484 .. 20539783
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28480.t1

Sequence Viewer

Length: 462 bp
ATGAGTCGGCACCCGGAGGTTCTATGGGCGCAGCGGTCCGATAAAGTATACCTTACGGTGGCGTTACCGGATGCGAAAGACATTAACGTCAAGTGTGAGGCTGAAGGCTTGTTCTCCTTCTCCGCCACCGGACTCCAAGGCCACCCTTTTCACTTCACTCTCGACCTTTACGGCCAAATACAACCCCAGGGTTGTAAGACTAAGGCCGGCTCAAGGAACATCCTCTGCTCCATCCAGAAACAACACAAAGGTTGGTGGAAACGACTTCTCAAAACCCAAGAAAAACCTGCCCCTTACATTAAGGTCGATTGGAACAATTGGTGCGACGAGGACGACGAACAAGACTCTACTTCTGATTTGGCCTCAGATGATGATGATGCTACGATCGGTGAAGATGATGAAAGCAGTGAAGATGAAGGAATGCTCTATCTGCCCGACCTCGAGAAGGCAAGAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko01100 map00590 map01100
Pfam Domains
Protein Families
CS

Protein Analysis

154

Amino Acids

17.42

Weight (kDa)

4.79

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 6 - 80 2e-08 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03773 AT3G03773 AT3G03773
fragaria_vesca FvH4_1g28480
malus_domestica MD12G1008700.v1.1
prunus_persica Prupe.7G010500_v2.0.a1
pyrus_communis pycom12g00650
rosa_chinensis RchiOBHm_Chr3g0496351
rosa_laevigata RLG00000022529
rosa_multiflora Rmu_co8304491.1_g000001 Rmu_sc0002461.1_g000005
rosa_roxburghii Rroxscaffold_6G00389260
rosa_rugosa Rorug03G0281700 Rorug03G0281800 Rorug03G0281900
rosa_samantha Rh3AG328500 Rh3BG364600 Rh3CG361600 Rh3DG364600
rosa_wichuraiana Rw3G028760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 9
AccI GTMKAC 1 cut(s) 48
AciI CCGC 2 cut(s) 34, 123
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 58, 213, 445
AjnI CCWGG 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 4 cut(s) 139, 172, 204, 360
ApeKI GCWGC 1 cut(s) 31
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 36
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 36
BanI GGYRCC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 188
BcnI CCSGG 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 295
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 2 cut(s) 14, 188
Bme18I GGWCC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 11
BmrFI CCNGG 2 cut(s) 14, 188
BmsI GCATC 2 cut(s) 61, 367
BpuEI CTTGAG 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 136, 186, 187
BsaWI WCCGGW 2 cut(s) 67, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 213, 445
Bse118I RCCGGY 1 cut(s) 206
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 136, 186, 187
BseGI GGATG 3 cut(s) 76, 219, 231
BseLI CCNNNNNNNGG 3 cut(s) 58, 213, 445
BseMII CTCAG 1 cut(s) 378
BseXI GCAGC 1 cut(s) 43
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 4 cut(s) 141, 174, 206, 362
BshNI GGYRCC 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 4 cut(s) 14, 68, 129, 207
BslI CCNNNNNNNGG 3 cut(s) 58, 213, 445
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 4 cut(s) 141, 174, 206, 362
BsoBI CYCGRG 1 cut(s) 440
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 2 cut(s) 34, 123
BspANI GGCC 4 cut(s) 141, 174, 206, 362
BspCNI CTCAG 1 cut(s) 377
BspLI GGNNCC 1 cut(s) 11
BspMI ACCTGC 1 cut(s) 295
BspT107I GGYRCC 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 136, 186, 187
BssMI GATC 1 cut(s) 384
BssNAI GTATAC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 136
Bst1107I GTATAC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 201, 364
BstENI CCTNNNNNAGG 1 cut(s) 443
BstF5I GGATG 3 cut(s) 76, 219, 231
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 1 cut(s) 387
BstMBI GATC 1 cut(s) 384
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 12, 186
BstV1I GCAGC 1 cut(s) 43
BstZ17I GTATAC 1 cut(s) 49
BsuRI GGCC 4 cut(s) 141, 174, 206, 362
BtsCI GGATG 3 cut(s) 76, 219, 231
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 36
CspI CGGWCCG 1 cut(s) 36
CviJI RGCY 7 cut(s) 101, 108, 141, 174, 206, 210, 362
CviKI_1 RGCY 7 cut(s) 101, 108, 141, 174, 206, 210, 362
DdeI CTNAG 2 cut(s) 201, 364
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 172
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaiI YATR 2 cut(s) 25, 49
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 3 cut(s) 83, 206, 218
Fsp4HI GCNGC 1 cut(s) 32
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 32
HaeIII GGCC 4 cut(s) 141, 174, 206, 362
HapII CCGG 4 cut(s) 14, 68, 129, 207
HhaI GCGC 1 cut(s) 31
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HinfI GANTC 3 cut(s) 4, 132, 344
HpaII CCGG 4 cut(s) 14, 68, 129, 207
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 40, 355, 367
Hpy188III TCNNGA 3 cut(s) 161, 235, 442
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 2 cut(s) 329, 338
HpyAV CCTTC 4 cut(s) 98, 127, 410, 439
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 2 cut(s) 201, 364
HpySE526I ACGT 1 cut(s) 87
HspAI GCGC 1 cut(s) 29
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 8 cut(s) 27, 81, 142, 173, 200, 220, 248, 300
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 2 cut(s) 61, 367
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 63
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 2 cut(s) 404, 422
MfeI CAATTG 1 cut(s) 316
MluCI AATT 1 cut(s) 316
MlyI GAGTC 3 cut(s) 13, 126, 338
MnlI CCTC 6 cut(s) 10, 91, 233, 322, 373, 449
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 2 cut(s) 84, 300
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 4 cut(s) 14, 68, 129, 207
MspR9I CCNGG 2 cut(s) 14, 188
MunI CAATTG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 430
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 14
NdeII GATC 1 cut(s) 384
NgoMIV GCCGGC 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 440
PasI CCCWGGG 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 33
Ple19I CGATCG 1 cut(s) 387
PleI GAGTC 3 cut(s) 12, 126, 338
PpsI GAGTC 3 cut(s) 12, 126, 338
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 36
PvuI CGATCG 1 cut(s) 387
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 84, 300
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 1 cut(s) 36
SchI GAGTC 3 cut(s) 13, 126, 338
ScrFI CCNGG 2 cut(s) 14, 188
SetI ASST 8 cut(s) 21, 54, 90, 168, 253, 289, 306, 441
SfaNI GCATC 2 cut(s) 61, 367
Sfr274I CTCGAG 1 cut(s) 440
SinI GGWCC 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 211, 440
SmoI CTYRAG 2 cut(s) 211, 440
Sse9I AATT 1 cut(s) 316
SsiI CCGC 2 cut(s) 34, 123
StyD4I CCNGG 2 cut(s) 12, 186
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 90
TaqI TCGA 3 cut(s) 162, 306, 441
TasI AATT 1 cut(s) 316
Tru1I TTAA 2 cut(s) 84, 300
Tru9I TTAA 2 cut(s) 84, 300
TscAI CASTG 1 cut(s) 412
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 414, 429
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 36
XagI CCTNNNNNAGG 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.