RLG00000022529

CS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011066
Physical Location & Seq
Reverse (-)
2900450 .. 2902697
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022529

Sequence Viewer

Length: 480 bp
ATGAGTCGGCACCCGGAGGTTCTTTGGGCGCAGCGGTCCGATAAAGTATACCTGACGGTGGCGTTACCGGATGCGAAAGACATAAGCGTCAAGTGTGAGCCCGAGGGCTTGTTCGCCTTCTCCGCCACCGGAGTCCAAGGAGAGTCCTTTCACTTCACTTTGGAGCTCTACGGACAGATACTACCTCAGGGTTGTAAAACTAAGGCTGGCTCAAGGAACATCCTCTGCTCCATCCAGAAACTACACAAAGCTTGGTGGCAACGACTTCTCAAGACCCAAGAAAAACCTGCCCCTTACATTAAGGTCGATTGGAACAATTGGTGCGACGAGGAGGACGACGAAATAGACTCCACTTCTAAATTGGCCTCTGATGATGATGATGATGATGATGATGCTGCGAATATCGGTGAAGATGATGGAAGCAGCGAAGATGAAGGAATGCTCTATCTTCCCGACCTGGAGAAGGCAAGAAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko01100 map00590 map01100
Pfam Domains
Protein Families
CS

Protein Analysis

160

Amino Acids

17.97

Weight (kDa)

4.52

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 6 - 80 6.5e-08 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03773 AT3G03773 AT3G03773
fragaria_vesca FvH4_1g28480
malus_domestica MD12G1008700.v1.1
prunus_persica Prupe.7G010500_v2.0.a1
pyrus_communis pycom12g00650
rosa_chinensis RchiOBHm_Chr3g0496351
rosa_laevigata RLG00000022529
rosa_multiflora Rmu_co8304491.1_g000001 Rmu_sc0002461.1_g000005
rosa_roxburghii Rroxscaffold_6G00389260
rosa_rugosa Rorug03G0281700 Rorug03G0281800 Rorug03G0281900
rosa_samantha Rh3AG328500 Rh3BG364600 Rh3CG361600 Rh3DG364600
rosa_wichuraiana Rw3G028760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 9
AccI GTMKAC 1 cut(s) 48
AciI CCGC 2 cut(s) 34, 123
AfiI CCNNNNNNNGG 2 cut(s) 58, 463
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 2 cut(s) 166, 251
AluI AGCT 2 cut(s) 166, 251
Alw21I GWGCWC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 3 cut(s) 31, 395, 423
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 36
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 1 cut(s) 36
AxyI CCTNAGG 1 cut(s) 186
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 2 cut(s) 102, 168
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 3 cut(s) 43, 382, 435
BccI CCATC 2 cut(s) 239, 410
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 295
BisI GCNGC 3 cut(s) 32, 396, 424
BlsI GCNGC 3 cut(s) 33, 397, 425
Bme1390I CCNGG 2 cut(s) 14, 458
Bme18I GGWCC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 11
BmrFI CCNGG 2 cut(s) 14, 458
BmsI GCATC 2 cut(s) 61, 382
BpmI CTGGAG 1 cut(s) 479
BpuEI CTTGAG 2 cut(s) 196, 254
BpuMI CCSGG 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 102, 136
BsaWI WCCGGW 2 cut(s) 67, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 463
Bse21I CCTNAGG 1 cut(s) 186
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 102, 136
BseGI GGATG 3 cut(s) 76, 219, 231
BseLI CCNNNNNNNGG 2 cut(s) 58, 463
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 43, 382, 435
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 3 cut(s) 14, 68, 129
BslI CCNNNNNNNGG 2 cut(s) 58, 463
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 102, 168
BspACI CCGC 2 cut(s) 34, 123
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 11
BspMI ACCTGC 1 cut(s) 295
BspT107I GGYRCC 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 102, 136
BssNAI GTATAC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 136
Bst1107I GTATAC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 186, 201
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 3 cut(s) 76, 219, 231
BstHHI GCGC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 12, 456
BstV1I GCAGC 3 cut(s) 43, 382, 435
BstZ17I GTATAC 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 365
BtsCI GGATG 3 cut(s) 76, 219, 231
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 36
CseI GACGC 1 cut(s) 76
CspI CGGWCCG 1 cut(s) 36
CviJI RGCY 7 cut(s) 100, 108, 166, 206, 210, 251, 365
CviKI_1 RGCY 7 cut(s) 100, 108, 166, 206, 210, 251, 365
DdeI CTNAG 2 cut(s) 186, 201
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 102, 168
Eco47I GGWCC 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 102, 168
ErhI CCWWGG 1 cut(s) 136
FaiI YATR 2 cut(s) 49, 83
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 32, 396, 424
FokI GGATG 3 cut(s) 83, 206, 218
FriOI GRGCYC 2 cut(s) 102, 168
Fsp4HI GCNGC 3 cut(s) 32, 396, 424
GlaI GCGC 1 cut(s) 30
GluI GCNGC 3 cut(s) 32, 396, 424
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 1 cut(s) 365
HapII CCGG 3 cut(s) 14, 68, 129
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 31
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 4 cut(s) 4, 132, 143, 347
HpaII CCGG 3 cut(s) 14, 68, 129
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 40, 370
Hpy188III TCNNGA 3 cut(s) 235, 271, 452
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 2 cut(s) 329, 341
HpyAV CCTTC 3 cut(s) 127, 428, 457
HpyCH4III ACNGT 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 2 cut(s) 186, 201
HspAI GCGC 1 cut(s) 29
LmnI GCTCC 2 cut(s) 163, 233
Lsp1109I GCAGC 3 cut(s) 43, 382, 435
LweI GCATC 2 cut(s) 61, 382
MaeIII GTNAC 1 cut(s) 63
MboII GAAGA 3 cut(s) 422, 440, 440
MfeI CAATTG 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 102, 168
MluCI AATT 2 cut(s) 316, 359
MlyI GAGTC 4 cut(s) 13, 141, 152, 341
MnlI CCTC 7 cut(s) 10, 97, 195, 233, 322, 325, 376
MseI TTAA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 3 cut(s) 14, 68, 129
MspR9I CCNGG 2 cut(s) 14, 458
MunI CAATTG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 122
NciI CCSGG 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 1 cut(s) 444
PkrI GCNGC 3 cut(s) 33, 397, 425
PleI GAGTC 4 cut(s) 12, 140, 151, 341
PpsI GAGTC 4 cut(s) 12, 140, 151, 341
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 1 cut(s) 11
PspPI GGNCC 1 cut(s) 36
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 3 cut(s) 32, 396, 424
Sau96I GGNCC 1 cut(s) 36
SchI GAGTC 4 cut(s) 13, 141, 152, 341
ScrFI CCNGG 2 cut(s) 14, 458
SduI GDGCHC 2 cut(s) 102, 168
SetI ASST 8 cut(s) 21, 54, 168, 187, 253, 289, 306, 459
SfaNI GCATC 2 cut(s) 61, 382
SinI GGWCC 1 cut(s) 36
SmlI CTYRAG 2 cut(s) 211, 269
SmoI CTYRAG 2 cut(s) 211, 269
Sse9I AATT 2 cut(s) 316, 359
SsiI CCGC 2 cut(s) 34, 123
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 12, 456
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 1 cut(s) 306
TasI AATT 2 cut(s) 316, 359
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseI GCWGC 3 cut(s) 31, 395, 423
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 36
XagI CCTNNNNNAGG 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.