MD12G1008700.v1.1

CS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011066
Physical Location & Seq
Reverse (-)
933240 .. 936166
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1008700.v1.1.491

Sequence Viewer

Length: 462 bp
ATGAGTGGGACCAGTCGGCACCCGGAGGTTCTGTGGGCGCAGCGCTCCGACAAGGTGTACCTGACGGTGGCGCTACCGGACGCCAAAGACATCACCGTCAAGTGCGAGCCTGAGGGATTATTCAGCTTCTCCGCCGTGGGAGTCCAAGGCGAGCCCTTTCACTTCACTCTCGAGCTGTACGGTCCAATAACCCCTGACGGCTGCAAAACCAGAACTGGGTTGCGGAACATTCTCTGCTCGATCCAGAAGGAGCAGAGAGGTTGGTGGAAGCGGTTGCTGAAGACGGAAGAGAAGCCGGCGCCCTACATTAAAGTTGATTGGAACAAATGGTGCGATGAGGACGAGGAAGCTGATTTGGCCCCCGATGATGATGATGATGATGCGTATGTTGGTCAAGATGGTGGAAGCAGTGACGACGAAGGAATGCTCTATCTTCCGGACCTGGAGAAGGCGAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko01100 map00590 map01100
Pfam Domains
Protein Families
CS

Protein Analysis

154

Amino Acids

17.3

Weight (kDa)

4.56

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 9 - 83 2.9e-08 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03773 AT3G03773 AT3G03773
fragaria_vesca FvH4_1g28480
malus_domestica MD12G1008700.v1.1
prunus_persica Prupe.7G010500_v2.0.a1
pyrus_communis pycom12g00650
rosa_chinensis RchiOBHm_Chr3g0496351
rosa_laevigata RLG00000022529
rosa_multiflora Rmu_co8304491.1_g000001 Rmu_sc0002461.1_g000005
rosa_roxburghii Rroxscaffold_6G00389260
rosa_rugosa Rorug03G0281700 Rorug03G0281800 Rorug03G0281900
rosa_samantha Rh3AG328500 Rh3BG364600 Rh3CG361600 Rh3DG364600
rosa_wichuraiana Rw3G028760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 18, 298
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 3 cut(s) 132, 223, 271
AclWI GGATC 1 cut(s) 235
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 2 cut(s) 81, 299
AfaI GTAC 2 cut(s) 59, 179
AfeI AGCGCT 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 67, 216, 448
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 3 cut(s) 126, 175, 350
AluI AGCT 3 cut(s) 126, 175, 350
AlwI GGATC 1 cut(s) 235
Ama87I CYCGRG 1 cut(s) 170
Aor13HI TCCGGA 1 cut(s) 436
Aor51HI AGCGCT 1 cut(s) 44
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 2 cut(s) 40, 201
AspLEI GCGC 4 cut(s) 40, 45, 73, 301
AspS9I GGNCC 4 cut(s) 9, 182, 358, 439
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 3 cut(s) 9, 182, 439
AxyI CCTNAGG 1 cut(s) 111
BanI GGYRCC 2 cut(s) 18, 298
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 52, 188
BccI CCATC 1 cut(s) 392
BceAI ACGGC 2 cut(s) 119, 214
BciT130I CCWGG 1 cut(s) 443
BcnI CCSGG 1 cut(s) 23
BfoI RGCGCY 3 cut(s) 46, 74, 302
BisI GCNGC 2 cut(s) 41, 202
BlsI GCNGC 2 cut(s) 42, 203
Bme1390I CCNGG 2 cut(s) 23, 443
Bme18I GGWCC 3 cut(s) 9, 182, 439
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 4 cut(s) 9, 182, 358, 439
BmiI GGNNCC 4 cut(s) 10, 20, 300, 360
BmrFI CCNGG 2 cut(s) 23, 443
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 1 cut(s) 370
BmuI ACTGGG 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 23
BsaHI GRCGYC 2 cut(s) 81, 299
BsaJI CCNNGG 2 cut(s) 135, 145
BsaWI WCCGGW 2 cut(s) 76, 436
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 216, 448
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 12, 220
Bse21I CCTNAGG 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 135, 145
BseLI CCNNNNNNNGG 3 cut(s) 67, 216, 448
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 12, 220
BseXI GCAGC 2 cut(s) 52, 188
BshFI GGCC 1 cut(s) 359
BshNI GGYRCC 2 cut(s) 18, 298
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 4 cut(s) 23, 77, 296, 437
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 3 cut(s) 67, 216, 448
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 1 cut(s) 240
BspACI CCGC 3 cut(s) 132, 223, 271
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 1 cut(s) 103
BspEI TCCGGA 1 cut(s) 436
BspLI GGNNCC 4 cut(s) 10, 20, 300, 360
BspPI GGATC 1 cut(s) 235
BspT107I GGYRCC 2 cut(s) 18, 298
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 2 cut(s) 12, 220
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 135, 145
BssMI GATC 1 cut(s) 240
BssNI GRCGYC 2 cut(s) 81, 299
BssT1I CCWWGG 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 67, 97, 182
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 2 cut(s) 81, 299
BstC8I GCNNGC 3 cut(s) 107, 152, 297
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 1 cut(s) 135
BstENI CCTNNNNNAGG 1 cut(s) 446
BstH2I RGCGCY 3 cut(s) 46, 74, 302
BstHHI GCGC 4 cut(s) 40, 45, 73, 301
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 21, 441
BstV1I GCAGC 2 cut(s) 52, 188
BstV2I GAAGAC 1 cut(s) 287
Bsu36I CCTNAGG 1 cut(s) 111
BsuRI GGCC 1 cut(s) 359
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 348
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 107, 152, 297
CfoI GCGC 4 cut(s) 40, 45, 73, 301
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 4 cut(s) 9, 182, 358, 439
CseI GACGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 58, 178
CviJI RGCY 8 cut(s) 109, 126, 154, 175, 201, 295, 350, 359
CviKI_1 RGCY 8 cut(s) 109, 126, 154, 175, 201, 295, 350, 359
CviQI GTAC 2 cut(s) 58, 178
DdeI CTNAG 1 cut(s) 111
DinI GGCGCC 1 cut(s) 300
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 3 cut(s) 9, 182, 439
Eco47III AGCGCT 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 145
FaiI YATR 1 cut(s) 387
FaqI GGGAC 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 41, 202
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 2 cut(s) 41, 202
GlaI GCGC 4 cut(s) 39, 44, 72, 300
GluI GCNGC 2 cut(s) 41, 202
HaeII RGCGCY 3 cut(s) 46, 74, 302
HaeIII GGCC 1 cut(s) 359
HapII CCGG 4 cut(s) 23, 77, 296, 437
HgaI GACGC 1 cut(s) 89
HhaI GCGC 4 cut(s) 40, 45, 73, 301
Hin1I GRCGYC 2 cut(s) 81, 299
Hin6I GCGC 4 cut(s) 38, 43, 71, 299
HinP1I GCGC 4 cut(s) 38, 43, 71, 299
HinfI GANTC 1 cut(s) 141
HpaII CCGG 4 cut(s) 23, 77, 296, 437
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 49
Hpy188III TCNNGA 4 cut(s) 170, 244, 395, 437
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 3 cut(s) 241, 413, 442
HpyCH4III ACNGT 3 cut(s) 67, 97, 182
HpyCH4V TGCA 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 1 cut(s) 111
Hsp92I GRCGYC 2 cut(s) 81, 299
HspAI GCGC 4 cut(s) 38, 43, 71, 299
KasI GGCGCC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 436
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 2 cut(s) 50, 250
Lsp1109I GCAGC 2 cut(s) 52, 188
LweI GCATC 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 410
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MboII GAAGA 3 cut(s) 292, 299, 425
MhlI GDGCHC 1 cut(s) 156
Mly113I GGCGCC 1 cut(s) 299
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 5 cut(s) 19, 106, 251, 331, 337
MroI TCCGGA 1 cut(s) 436
MroNI GCCGGC 1 cut(s) 295
MseI TTAA 1 cut(s) 309
MspI CCGG 4 cut(s) 23, 77, 296, 437
MspR9I CCNGG 2 cut(s) 23, 443
MteI GCGCNGCGC 1 cut(s) 41
Mva1269I GAATGC 1 cut(s) 429
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 356
NaeI GCCGGC 1 cut(s) 297
NarI GGCGCC 1 cut(s) 299
NciI CCSGG 1 cut(s) 23
NdeII GATC 1 cut(s) 240
NgoMIV GCCGGC 1 cut(s) 295
NlaIV GGNNCC 4 cut(s) 10, 20, 300, 360
NmuCI GTSAC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 170
PctI GAATGC 1 cut(s) 429
PdiI GCCGGC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 42, 203
PleI GAGTC 1 cut(s) 149
PluTI GGCGCC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 4 cut(s) 10, 20, 300, 360
PspPI GGNCC 4 cut(s) 9, 182, 358, 439
RsaI GTAC 2 cut(s) 59, 179
RsaNI GTAC 2 cut(s) 58, 178
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 41, 202
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 4 cut(s) 9, 182, 358, 439
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 2 cut(s) 23, 443
SduI GDGCHC 1 cut(s) 156
SetI ASST 8 cut(s) 30, 57, 63, 128, 177, 262, 352, 444
SfaNI GCATC 1 cut(s) 370
SfoI GGCGCC 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 3 cut(s) 9, 182, 439
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SsiI CCGC 3 cut(s) 132, 223, 271
SspDI GGCGCC 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 21, 441
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 3 cut(s) 67, 97, 182
TaqI TCGA 2 cut(s) 171, 239
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 2 cut(s) 40, 201
Tsp45I GTSAC 1 cut(s) 410
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 9, 182, 439
XagI CCTNNNNNAGG 1 cut(s) 446
XhoI CTCGAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.