Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009188

Physical Location & Seq

Genomic Coordinates Forward (+)

22108635 .. 22120594

Gene Structure 11960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g29260.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2874 bp

ATGGTACAACTCAAGTCCAGCAAACCGCTGAGTGTGAGCTTCAGAATCCCGTATTACACTCAGTGGGGCCAGAGCCTGGTGGTGTGCGGCTCCGAACCGGTGCTGGGTTCATGGAATGTGAAGAGGGGTCTGCAGTTGAGTCCTGTTCATCAGGGTGATGAGCTCATATGGTTCGGCACCCTCTCTATCCCCAAAGGCTTTGGCCCATGCGAATACAGTTACTACGTTGTGGATGATGACAGAAATGTTGTGCGATGGGAGATGGGAAAAAAGCGTCGCCTTCTGTTGCCTCAGACCTTTGAGGGAGGAGAGCAGCTTCACTTGCATGACTTTTGGCAGGTTGGTGCCGATGCACTTCCTTTCAAAAGTGCCTTCAAGGACGTCGTCTTTCGTAGAGAGTGCACTTTGGAGATTGAGAAACCTCTTGGGCTCATCCAAAATTCATTGCAGAATGATGTTTGCTGTCCAAATTTACAAGAAGGAACACCGATTTACATTATTGGTAGTTGCTCAAAGCTAGGGAATTGGAAGGCGCAGGATGGGCTTAAACTATTTTATGCTGGTGATTCAACTTGGCATGCAGACTGTGTCTTGCCCAAAGGGGACTTCCCGATAAAATATAAGTACTGTAAATGTAGCAAAGGAGGAAACATTTCTTCTGAAACTGGTCCAAACCGCGAAATTGCTCTCGACTCGTCAATTACTGAACCGAGATATCTATTCCGTTCAGATGGCATGTTGCAAGAACTGCCTTGGCGGGGTGCTGGTGTTGCAATCCCAATGTTCTCTGTTAGATCAGAAACTGATCTTGGTGTCGGAGAGTTTCTTGACCTGAAATTACTTGCTGATTGGGCTGTAGAGTCTGGCTTTCATTTAGTTCAGCTCTTACCCATAAATGACACATCTGTGTATGGAATGTGGTGGGATTCATATCCTTACAGCTCACTTTCAGTATCTGCCTTGCATCCTTTATACTTGAGAGTACAGGCACTTTCAGAAAACATCCCCATGGATATCAAGAAGGCAAAAGAAGAATTGGACGGAAAGGATGTTGATTACGAGGCTACCTTGATCACTAAACTCTCAATTGGAAAGAAAATATTTGCCCAAGAGAAGGACAAGATTCTCACCTCCAATTCCTTTCAGAATTTTTTTTCTGAGAATCAGGAATGGTTAAAACCCTATGCGGCCTTCTGTTTTTTGCGGGACTTCTTTGAAACATCAGATCACAGCCAATGGGGTCGGTTTTCCCAGTTTTCAATAGAAAAGCTAGAGAAACTCATCTCGAAAGACAGCATTCACTATGGTGTAATTTGCTTCCATTATTACATCCAATATCATTTGCACATGCAGCTGTCGGAAGCTGCAGAATATGCAAGAAAGAAAGGAGTAATATTGAAAGGAGATCTGCCTATAGGTGTCGGAAGAAACAGTGTAGATACATGGGTCTATCCAAATTTGTTTCGCATGAACACTTCTACTGGAGCTCCTCCAGATTATTTTGATAAAAATGGTCAGAACTGGGGTTTCCCTACTTACAATTGGGAAGAGATGGCGAAAGACAACTATGCCTGGTGGCGTGCTCGATTGACACAGATGGCAAAATACTTTACAGCTTACCGGATTGACCACATATTGGGGTTCTTTCGCATCTGGGAACTCCCAGAGCATGCCATGACTGGTCTCGTTGGAAAATTTCGACCTTCCATTCCTCTAAGTCAGGAAGAACTTGAAAGAGATGGCATATGGGACTTTGATCGCTTGGCTCGTCCATATATCCCACAGGAACTTTTACAGATAAAGTTTGGAGATTCATGGACTTTTATTGCTTCAAGTTTCCTGAATGAATATCAGAAGAACCGCTATGAGTTTAAGGAGGACTGCAACACTGAGAAAAAAATTGCTTCCAAGCTGAAGTCATTTTCAGGAAGATCTTTGTTGCAGAATGAAGATCATATTAGACAGGAGCTCTTTGATATTTTACAGAATATAGTTCTGATAAGAGATCCAGAAAATCCGAGGAACTTCTATCCTCGCTTCAATCTTGAAGAGACTTCAAGTTTTAAGGATTTGGATGATCACTGCAAGAATGTACTGAAACGGCTATACTATGATTACTACTTTCATCGTCAAGAAATTCTTTGGCGTGAAAATGCATTGAAGACACTGCCTGCTCTGTTGAACTCATCTGATATGCTAGCCTGTGGGGAAGATCTGGGGCTTATTCCTTCTTGTGTTTATCCTGTGATGCAAGAACTGGGATTAATAGGTTTACGCATTCAACGTATGCCTAGTGAACCTGGTCTGGAGTTTGGTATCCCTTCTCAATACAGTTATATGACGGTGTGCGCTCCATCATGCCATGACTGTTCAACGTTACGTGCTTGGTGGGAAGAAGATGAAGAAAGGAGGCAGCGATATTTTAGCAGCATGGTGGGATCTGACTTGTTGCCACCGAGTCGCTGTGTTCCAGAGATTGCAAATTTCATCATACGGCAACATTTTGAAGCTCCATCAATGTGGGCAATTTTCCCTCTTCAGGATTTGTTGATATTGAAAGAAGAATACACAACACGCCCTGCAAAAGAGGAGACAATCAATGACCCCACAAATCCGAAGCATTATTGGAGATACCGTGTGCATGTGACATTGGAGGCTTTGATCAAGGACAAAGAGCTCACATCAATCATCAAAGACCTCGTGCTTGGAAGTGGAAGATCGCATCCGGGGAAACATGCTGAGAAACAAGTGATTCCGGAATCAGCTATTGCAACCACAGAGAAAAAGCAGATTGCCAGCGGCAAGGAAAAGGCTAACGTTGCAACTCCACTTAATGGAGTTCCGCGGAAGGAGGCTGTGGCTGTTCCTAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0010297 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0030246 GO:0030247 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

CBM_20 Glyco_hydro_77

Protein Analysis

958

Amino Acids

110.28

Weight (kDa)

6.06

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	11 - 100	5.3e-21	Starch binding domain
CBM_20	PF00686	156 - 242	2.8e-14	Starch binding domain
Glyco_hydro_77	PF02446	267 - 358	3e-18	4-alpha-glucanotransferase
Glyco_hydro_77	PF02446	363 - 820	3.1e-98	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40840
fragaria_vesca	FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260
malus_domestica	MD00G1025200.v1.1
prunus_persica	Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1
pyrus_communis	pycom12g09050
rosa_chinensis	RchiOBHm_Chr3g0494731
rosa_laevigata	RLG00000022663
rosa_multiflora	Rmu_sc0000198.1_g000016
rosa_roxburghii	Rroxscaffold_6G00390950
rosa_rugosa	Rorug03G0268100
rosa_samantha	Rh3AG316600 Rh3BG351900 Rh3CG349000 Rh3DG351500
rosa_wichuraiana	Rw3G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	384
Acc36I	ACCTGC	1 cut(s)	328
AccB1I	GGYRCC	2 cut(s)	176, 344
AccB7I	CCANNNNNTGG	3 cut(s)	76, 1636, 2834
AccII	CGCG	2 cut(s)	678, 2845
AccIII	TCCGGA	1 cut(s)	2755
AclI	AACGTT	2 cut(s)	2375, 2817
AclWI	GGATC	2 cut(s)	2000, 2446
AcsI	RAATTY	7 cut(s)	439, 469, 1147, 1456, 1694, 2136, 2482
AcuI	CTGAAG	3 cut(s)	25, 1934, 2522
AcyI	GRCGYC	1 cut(s)	381
AdeI	CACNNNGTG	1 cut(s)	63
AfaI	GTAC	4 cut(s)	6, 626, 984, 2094
AfiI	CCNNNNNNNGG	7 cut(s)	76, 104, 758, 1114, 1636, 2539, 2834
AgeI	ACCGGT	1 cut(s)	97
AjnI	CCWGG	3 cut(s)	75, 1571, 2299
AjuI	GAANNNNNNNTTGG	4 cut(s)	590, 622, 792, 824
AleI	CACNNNNGTG	2 cut(s)	905, 1305
Alw21I	GWGCWC	6 cut(s)	165, 404, 1489, 1585, 1971, 2679
Alw26I	GTCTC	3 cut(s)	1688, 2045, 2585
Alw44I	GTGCAC	1 cut(s)	400
AlwI	GGATC	2 cut(s)	2000, 2446
AlwNI	CAGNNNCTG	3 cut(s)	76, 803, 956
Aor13HI	TCCGGA	1 cut(s)	2755
AoxI	GGCC	3 cut(s)	67, 202, 1188
ApaLI	GTGCAC	1 cut(s)	400
ApeKI	GCWGC	5 cut(s)	313, 1351, 1364, 2413, 2427
ApoI	RAATTY	7 cut(s)	439, 469, 1147, 1456, 1694, 2136, 2482
AseI	ATTAAT	1 cut(s)	2264
AsiGI	ACCGGT	1 cut(s)	97
Asp700I	GAANNNNTTC	1 cut(s)	652
AspLEI	GCGC	2 cut(s)	535, 2351
AspS9I	GGNCC	3 cut(s)	67, 203, 668
AsuC2I	CCSGG	1 cut(s)	2727
AsuHPI	GGTGA	3 cut(s)	167, 575, 1120
AsuNHI	GCTAGC	1 cut(s)	2197
AvaII	GGWCC	1 cut(s)	668
BaeGI	GKGCMC	1 cut(s)	404
BaeI	ACNNNNGTAYC	2 cut(s)	1431, 1464
BanI	GGYRCC	2 cut(s)	176, 344
BanII	GRGCYC	5 cut(s)	165, 432, 1489, 1971, 2679
BauI	CACGAG	1 cut(s)	2699
BbsI	GAAGAC	1 cut(s)	2168
Bbv12I	GWGCWC	6 cut(s)	165, 404, 1489, 1585, 1971, 2679
BbvI	GCAGC	5 cut(s)	325, 1351, 1363, 2425, 2439
BccI	CCATC	9 cut(s)	249, 256, 533, 725, 1546, 1591, 1733, 2362, 2521
BceAI	ACGGC	2 cut(s)	2117, 2510
BciT130I	CCWGG	3 cut(s)	77, 1573, 2301
BciVI	GTATCC	1 cut(s)	2327
BclI	TGATCA	3 cut(s)	1071, 2077, 2661
BcnI	CCSGG	1 cut(s)	2727
BcoDI	GTCTC	3 cut(s)	1688, 2045, 2585
BfaI	CTAG	4 cut(s)	518, 1271, 2198, 2292
BfmI	CTRYAG	4 cut(s)	131, 855, 1365, 1413
BfuAI	ACCTGC	1 cut(s)	328
BfuI	GTATCC	1 cut(s)	2327
BglII	AGATCT	3 cut(s)	1405, 1931, 2212
BisI	GCNGC	8 cut(s)	88, 314, 1188, 1352, 1365, 2414, 2428, 2800
BlsI	GCNGC	8 cut(s)	89, 315, 1189, 1353, 1366, 2415, 2429, 2801
BmcAI	AGTACT	1 cut(s)	626
Bme1390I	CCNGG	4 cut(s)	77, 1573, 2301, 2727
Bme18I	GGWCC	1 cut(s)	668
BmgT120I	GGNCC	3 cut(s)	67, 203, 668
BmiI	GGNNCC	4 cut(s)	68, 91, 178, 346
BmrFI	CCNGG	4 cut(s)	77, 1573, 2301, 2727
BmrI	ACTGGG	3 cut(s)	1246, 1531, 2267
BmsI	GCATC	5 cut(s)	340, 973, 1659, 2238, 2731
BmtI	GCTAGC	1 cut(s)	2201
BmuI	ACTGGG	3 cut(s)	1246, 1531, 2267
BpiI	GAAGAC	1 cut(s)	2168
BpmI	CTGGAG	3 cut(s)	1476, 1503, 2327
BpuEI	CTTGAG	1 cut(s)	997
BpuMI	CCSGG	1 cut(s)	2727
BsaAI	YACGTR	1 cut(s)	2381
BsaBI	GATNNNNATC	1 cut(s)	930
BsaHI	GRCGYC	1 cut(s)	381
BsaI	GGTCTC	1 cut(s)	1688
BsaJI	CCNNGG	5 cut(s)	752, 1008, 2018, 2726, 2843
BsaWI	WCCGGW	3 cut(s)	97, 1620, 2755
BsaXI	ACNNNNNCTCC	4 cut(s)	1471, 1501, 2620, 2650
Bsc4I	CCNNNNNNNGG	7 cut(s)	76, 104, 758, 1114, 1636, 2539, 2834
Bse118I	RCCGGY	1 cut(s)	97
Bse1I	ACTGG	6 cut(s)	670, 1252, 1486, 1526, 1684, 2262
Bse3DI	GCAATG	1 cut(s)	443
Bse8I	GATNNNNATC	1 cut(s)	930
BseAI	TCCGGA	1 cut(s)	2755
BseBI	CCWGG	3 cut(s)	77, 1573, 2301
BseDI	CCNNGG	5 cut(s)	752, 1008, 2018, 2726, 2843
BseGI	GGATG	9 cut(s)	238, 432, 544, 964, 1002, 1054, 1329, 2080, 2722
BseJI	GATNNNNATC	1 cut(s)	930
BseLI	CCNNNNNNNGG	7 cut(s)	76, 104, 758, 1114, 1636, 2539, 2834
BseMI	GCAATG	1 cut(s)	443
BseMII	CTCAG	6 cut(s)	20, 74, 305, 1149, 1881, 2730
BseNI	ACTGG	6 cut(s)	670, 1252, 1486, 1526, 1684, 2262
BseRI	GAGGAG	3 cut(s)	321, 1479, 2603
BseSI	GKGCMC	1 cut(s)	404
BseXI	GCAGC	5 cut(s)	325, 1351, 1363, 2425, 2439
BseYI	CCCAGC	1 cut(s)	103
Bsh1236I	CGCG	2 cut(s)	678, 2845
BshFI	GGCC	3 cut(s)	69, 204, 1190
BshNI	GGYRCC	2 cut(s)	176, 344
BshTI	ACCGGT	1 cut(s)	97
BsiHKAI	GWGCWC	6 cut(s)	165, 404, 1489, 1585, 1971, 2679
BsiSI	CCGG	4 cut(s)	98, 1621, 2726, 2756
BslFI	GGGAC	3 cut(s)	617, 1220, 1763
BslI	CCNNNNNNNGG	7 cut(s)	76, 104, 758, 1114, 1636, 2539, 2834
BsmAI	GTCTC	3 cut(s)	1688, 2045, 2585
BsmFI	GGGAC	3 cut(s)	617, 1220, 1763
BsmI	GAATGC	2 cut(s)	1296, 2277
BsnI	GGCC	3 cut(s)	69, 204, 1190
Bso31I	GGTCTC	1 cut(s)	1688
Bsp1286I	GDGCHC	7 cut(s)	165, 404, 432, 1489, 1585, 1971, 2679
Bsp13I	TCCGGA	1 cut(s)	2755
Bsp19I	CCATGG	1 cut(s)	1008
BspANI	GGCC	3 cut(s)	69, 204, 1190
BspCNI	CTCAG	6 cut(s)	21, 73, 304, 1150, 1882, 2731
BspEI	TCCGGA	1 cut(s)	2755
BspFNI	CGCG	2 cut(s)	678, 2845
BspLI	GGNNCC	4 cut(s)	68, 91, 178, 346
BspMAI	CTGCAG	2 cut(s)	135, 1369
BspMI	ACCTGC	1 cut(s)	328
BspOI	GCTAGC	1 cut(s)	2201
BspPI	GGATC	2 cut(s)	2000, 2446
BspT107I	GGYRCC	2 cut(s)	176, 344
BspTNI	GGTCTC	1 cut(s)	1688
BsrDI	GCAATG	1 cut(s)	443
BsrFI	RCCGGY	1 cut(s)	97
BsrI	ACTGG	6 cut(s)	670, 1252, 1486, 1526, 1684, 2262
BssAI	RCCGGY	1 cut(s)	97
BssECI	CCNNGG	5 cut(s)	752, 1008, 2018, 2726, 2843
BssNI	GRCGYC	1 cut(s)	381
BssSI	CACGAG	1 cut(s)	2699
BssT1I	CCWWGG	2 cut(s)	752, 1008
Bst2BI	CACGAG	1 cut(s)	2699
Bst2UI	CCWGG	3 cut(s)	77, 1573, 2301
Bst4CI	ACNGT	8 cut(s)	218, 587, 629, 1433, 2333, 2344, 2369, 2636
Bst6I	CTCTTC	4 cut(s)	116, 1542, 2043, 2541
BstACI	GRCGYC	1 cut(s)	381
BstAPI	GCANNNNNTGC	2 cut(s)	748, 1373
BstBAI	YACGTR	1 cut(s)	2381
BstC8I	GCNNGC	6 cut(s)	579, 1581, 1671, 2172, 2199, 2797
BstDEI	CTNAG	7 cut(s)	29, 60, 291, 1158, 1715, 1890, 2739
BstDSI	CCRYGG	2 cut(s)	1008, 2843
BstF5I	GGATG	9 cut(s)	238, 432, 544, 964, 1002, 1054, 1329, 2080, 2722
BstFNI	CGCG	2 cut(s)	678, 2845
BstHHI	GCGC	2 cut(s)	535, 2351
BstMAI	GTCTC	3 cut(s)	1688, 2045, 2585
BstMWI	GCNNNNNNNGC	8 cut(s)	322, 541, 748, 770, 851, 1351, 1373, 2819
BstNI	CCWGG	3 cut(s)	77, 1573, 2301
BstNSI	RCATGY	6 cut(s)	581, 739, 1351, 1673, 2645, 2738
BstSCI	CCNGG	4 cut(s)	75, 1571, 2299, 2725
BstSFI	CTRYAG	4 cut(s)	131, 855, 1365, 1413
BstSLI	GKGCMC	1 cut(s)	404
BstUI	CGCG	2 cut(s)	678, 2845
BstV1I	GCAGC	5 cut(s)	325, 1351, 1363, 2425, 2439
BstV2I	GAAGAC	1 cut(s)	2168
BstX2I	RGATCY	5 cut(s)	1405, 1931, 2005, 2212, 2438
BstXI	CCANNNNNNTGG	1 cut(s)	2520
BstYI	RGATCY	5 cut(s)	1405, 1931, 2005, 2212, 2438
BsuI	GTATCC	1 cut(s)	2327
BsuRI	GGCC	3 cut(s)	69, 204, 1190
BtgI	CCRYGG	2 cut(s)	1008, 2843
BtgZI	GCGATG	1 cut(s)	268
BtsCI	GGATG	9 cut(s)	238, 432, 544, 964, 1002, 1054, 1329, 2080, 2722
BtsI	GCAGTG	2 cut(s)	2080, 2165
BtsIMutI	CAGTG	5 cut(s)	68, 1438, 1887, 2080, 2165
BveI	ACCTGC	1 cut(s)	328
Cac8I	GCNNGC	6 cut(s)	579, 1581, 1671, 2172, 2199, 2797
CaiI	CAGNNNCTG	3 cut(s)	76, 803, 956
CfoI	GCGC	2 cut(s)	535, 2351
Cfr10I	RCCGGY	1 cut(s)	97
Cfr13I	GGNCC	3 cut(s)	67, 203, 668
Cfr42I	CCGCGG	1 cut(s)	2846
CseI	GACGC	1 cut(s)	263
CsiI	ACCWGGT	1 cut(s)	2299
Csp6I	GTAC	4 cut(s)	5, 625, 983, 2093
CspAI	ACCGGT	1 cut(s)	97
CviQI	GTAC	4 cut(s)	5, 625, 983, 2093
DdeI	CTNAG	7 cut(s)	29, 60, 291, 1158, 1715, 1890, 2739
DraIII	CACNNNGTG	1 cut(s)	63
Eam1104I	CTCTTC	4 cut(s)	116, 1542, 2043, 2541
EarI	CTCTTC	4 cut(s)	116, 1542, 2043, 2541
Ecl136II	GAGCTC	4 cut(s)	163, 1487, 1969, 2677
Eco130I	CCWWGG	2 cut(s)	752, 1008
Eco24I	GRGCYC	5 cut(s)	165, 432, 1489, 1971, 2679
Eco31I	GGTCTC	1 cut(s)	1688
Eco32I	GATATC	2 cut(s)	716, 1015
Eco47I	GGWCC	1 cut(s)	668
Eco53kI	GAGCTC	4 cut(s)	163, 1487, 1969, 2677
Eco57I	CTGAAG	3 cut(s)	25, 1934, 2522
EcoICRI	GAGCTC	4 cut(s)	163, 1487, 1969, 2677
EcoRII	CCWGG	3 cut(s)	75, 1571, 2299
EcoRV	GATATC	2 cut(s)	716, 1015
EcoT14I	CCWWGG	2 cut(s)	752, 1008
EcoT22I	ATGCAT	1 cut(s)	2158
EcoT38I	GRGCYC	5 cut(s)	165, 432, 1489, 1971, 2679
ErhI	CCWWGG	2 cut(s)	752, 1008
FalI	AAGNNNNNCTT	2 cut(s)	2124, 2156
FaqI	GGGAC	3 cut(s)	617, 1220, 1763
FauI	CCCGC	2 cut(s)	750, 1197
FauNDI	CATATG	2 cut(s)	167, 1745
FbaI	TGATCA	3 cut(s)	1071, 2077, 2661
Fnu4HI	GCNGC	8 cut(s)	88, 314, 1188, 1352, 1365, 2414, 2428, 2800
FokI	GGATG	9 cut(s)	245, 419, 551, 951, 989, 1061, 1316, 2087, 2709
FriOI	GRGCYC	5 cut(s)	165, 432, 1489, 1971, 2679
Fsp4HI	GCNGC	8 cut(s)	88, 314, 1188, 1352, 1365, 2414, 2428, 2800
FspBI	CTAG	4 cut(s)	518, 1271, 2198, 2292
GlaI	GCGC	2 cut(s)	534, 2350
GluI	GCNGC	8 cut(s)	88, 314, 1188, 1352, 1365, 2414, 2428, 2800
GsaI	CCCAGC	1 cut(s)	107
GsuI	CTGGAG	3 cut(s)	1476, 1503, 2327
HaeIII	GGCC	3 cut(s)	69, 204, 1190
HapII	CCGG	4 cut(s)	98, 1621, 2726, 2756
HgaI	GACGC	1 cut(s)	263
HhaI	GCGC	2 cut(s)	535, 2351
Hin1I	GRCGYC	1 cut(s)	381
Hin6I	GCGC	2 cut(s)	533, 2349
HinP1I	GCGC	2 cut(s)	533, 2349
HpaII	CCGG	4 cut(s)	98, 1621, 2726, 2756
HphI	GGTGA	3 cut(s)	167, 575, 1120
Hpy166II	GTNNAC	3 cut(s)	402, 2273, 2297
Hpy8I	GTNNAC	3 cut(s)	402, 2273, 2297
Hpy99I	CGWCG	2 cut(s)	279, 386
HpyCH4III	ACNGT	8 cut(s)	218, 587, 629, 1433, 2333, 2344, 2369, 2636
HpyCH4IV	ACGT	6 cut(s)	225, 381, 2284, 2375, 2380, 2817
HpyF10VI	GCNNNNNNNGC	8 cut(s)	322, 541, 748, 770, 851, 1351, 1373, 2819
HpyF3I	CTNAG	7 cut(s)	29, 60, 291, 1158, 1715, 1890, 2739
HpySE526I	ACGT	6 cut(s)	225, 381, 2284, 2375, 2380, 2817
Hsp92I	GRCGYC	1 cut(s)	381
HspAI	GCGC	2 cut(s)	533, 2349
Kpn2I	TCCGGA	1 cut(s)	2755
Ksp22I	TGATCA	3 cut(s)	1071, 2077, 2661
KspI	CCGCGG	1 cut(s)	2846
LmnI	GCTCC	6 cut(s)	95, 1484, 1492, 1966, 2356, 2515
Lsp1109I	GCAGC	5 cut(s)	325, 1351, 1363, 2425, 2439
LweI	GCATC	5 cut(s)	340, 973, 1659, 2238, 2731
MabI	ACCWGGT	1 cut(s)	2299
MaeI	CTAG	4 cut(s)	518, 1271, 2198, 2292
MaeII	ACGT	6 cut(s)	225, 381, 2284, 2375, 2380, 2817
MaeIII	GTNAC	3 cut(s)	218, 2376, 2644
MfeI	CAATTG	2 cut(s)	1086, 1540
MflI	RGATCY	5 cut(s)	1405, 1931, 2005, 2212, 2438
MhlI	GDGCHC	7 cut(s)	165, 404, 432, 1489, 1585, 1971, 2679
MlyI	GAGTC	4 cut(s)	148, 686, 869, 2467
MmeI	TCCRAC	4 cut(s)	796, 1338, 1402, 1669
Mph1103I	ATGCAT	1 cut(s)	2158
MroI	TCCGGA	1 cut(s)	2755
MroXI	GAANNNNTTC	1 cut(s)	652
MseI	TTAA	6 cut(s)	546, 1175, 1872, 2064, 2264, 2832
MslI	CAYNNNNRTG	6 cut(s)	153, 324, 905, 1007, 1305, 2518
MspA1I	CMGCKG	4 cut(s)	28, 1354, 2799, 2845
MspI	CCGG	4 cut(s)	98, 1621, 2726, 2756
MspR9I	CCNGG	4 cut(s)	77, 1573, 2301, 2727
MunI	CAATTG	2 cut(s)	1086, 1540
Mva1269I	GAATGC	2 cut(s)	1296, 2277
MvaI	CCWGG	3 cut(s)	77, 1573, 2301
MvnI	CGCG	2 cut(s)	678, 2845
MwoI	GCNNNNNNNGC	8 cut(s)	322, 541, 748, 770, 851, 1351, 1373, 2819
NciI	CCSGG	1 cut(s)	2727
NcoI	CCATGG	1 cut(s)	1008
NdeI	CATATG	2 cut(s)	167, 1745
NheI	GCTAGC	1 cut(s)	2197
NlaIV	GGNNCC	4 cut(s)	68, 91, 178, 346
NmuCI	GTSAC	1 cut(s)	2644
NsiI	ATGCAT	1 cut(s)	2158
NspI	RCATGY	6 cut(s)	581, 739, 1351, 1673, 2645, 2738
OliI	CACNNNNGTG	2 cut(s)	905, 1305
PaeI	GCATGC	2 cut(s)	581, 1673
PcsI	WCGNNNNNNNCGW	2 cut(s)	1765, 2281
PctI	GAATGC	2 cut(s)	1296, 2277
PdmI	GAANNNNTTC	1 cut(s)	652
PfeI	GAWTC	8 cut(s)	45, 566, 926, 1123, 1162, 1811, 2752, 2759
PflFI	GACNNNGTC	2 cut(s)	383, 587
PflMI	CCANNNNNTGG	3 cut(s)	76, 1636, 2834
PinAI	ACCGGT	1 cut(s)	97
PkrI	GCNGC	8 cut(s)	89, 315, 1189, 1353, 1366, 2415, 2429, 2801
PleI	GAGTC	4 cut(s)	147, 686, 868, 2466
PpsI	GAGTC	4 cut(s)	147, 686, 868, 2466
Ppu21I	YACGTR	1 cut(s)	2381
PshBI	ATTAAT	1 cut(s)	2264
Psp124BI	GAGCTC	4 cut(s)	165, 1489, 1971, 2679
Psp1406I	AACGTT	2 cut(s)	2375, 2817
Psp6I	CCWGG	3 cut(s)	75, 1571, 2299
PspFI	CCCAGC	1 cut(s)	103
PspGI	CCWGG	3 cut(s)	75, 1571, 2299
PspN4I	GGNNCC	4 cut(s)	68, 91, 178, 346
PspPI	GGNCC	3 cut(s)	67, 203, 668
PstI	CTGCAG	2 cut(s)	135, 1369
PstNI	CAGNNNCTG	3 cut(s)	76, 803, 956
PsuI	RGATCY	5 cut(s)	1405, 1931, 2005, 2212, 2438
PsyI	GACNNNGTC	2 cut(s)	383, 587
PvuII	CAGCTG	1 cut(s)	1354
RsaI	GTAC	4 cut(s)	6, 626, 984, 2094
RsaNI	GTAC	4 cut(s)	5, 625, 983, 2093
RseI	CAYNNNNRTG	6 cut(s)	153, 324, 905, 1007, 1305, 2518
SacI	GAGCTC	4 cut(s)	165, 1489, 1971, 2679
SacII	CCGCGG	1 cut(s)	2846
SaqAI	TTAA	6 cut(s)	546, 1175, 1872, 2064, 2264, 2832
SatI	GCNGC	8 cut(s)	88, 314, 1188, 1352, 1365, 2414, 2428, 2800
Sau96I	GGNCC	3 cut(s)	67, 203, 668
ScaI	AGTACT	1 cut(s)	626
SchI	GAGTC	4 cut(s)	148, 686, 869, 2467
ScrFI	CCNGG	4 cut(s)	77, 1573, 2301, 2727
SduI	GDGCHC	7 cut(s)	165, 404, 432, 1489, 1585, 1971, 2679
SexAI	ACCWGGT	1 cut(s)	2299
SfaNI	GCATC	5 cut(s)	340, 973, 1659, 2238, 2731
SfcI	CTRYAG	4 cut(s)	131, 855, 1365, 1413
Sfr303I	CCGCGG	1 cut(s)	2846
SgrBI	CCGCGG	1 cut(s)	2846
SinI	GGWCC	1 cut(s)	668
SmiMI	CAYNNNNRTG	6 cut(s)	153, 324, 905, 1007, 1305, 2518
SmlI	CTYRAG	2 cut(s)	11, 976
SmoI	CTYRAG	2 cut(s)	11, 976
SphI	GCATGC	2 cut(s)	581, 1673
SspI	AATATT	2 cut(s)	1101, 1395
SspMI	CTAG	4 cut(s)	518, 1271, 2198, 2292
SstI	GAGCTC	4 cut(s)	165, 1489, 1971, 2679
StyD4I	CCNGG	4 cut(s)	75, 1571, 2299, 2725
StyI	CCWWGG	2 cut(s)	752, 1008
TaaI	ACNGT	8 cut(s)	218, 587, 629, 1433, 2333, 2344, 2369, 2636
TaiI	ACGT	6 cut(s)	228, 384, 2287, 2378, 2383, 2820
TaqI	TCGA	4 cut(s)	690, 1286, 1585, 1699
TatI	WGTACW	3 cut(s)	624, 982, 2092
TauI	GCSGC	3 cut(s)	90, 1190, 2802
TfiI	GAWTC	8 cut(s)	45, 566, 926, 1123, 1162, 1811, 2752, 2759
Tru1I	TTAA	6 cut(s)	546, 1175, 1872, 2064, 2264, 2832
Tru9I	TTAA	6 cut(s)	546, 1175, 1872, 2064, 2264, 2832
TscAI	CASTG	5 cut(s)	68, 1438, 1894, 2087, 2172
TseFI	GTSAC	1 cut(s)	2644
TseI	GCWGC	5 cut(s)	313, 1351, 1364, 2413, 2427
Tsp45I	GTSAC	1 cut(s)	2644
TspGWI	ACGGA	2 cut(s)	713, 1056
TspRI	CASTG	5 cut(s)	68, 1438, 1894, 2087, 2172
Tth111I	GACNNNGTC	2 cut(s)	383, 587
Van91I	CCANNNNNTGG	3 cut(s)	76, 1636, 2834
VneI	GTGCAC	1 cut(s)	400
VpaK11BI	GGWCC	1 cut(s)	668
VspI	ATTAAT	1 cut(s)	2264
XapI	RAATTY	7 cut(s)	439, 469, 1147, 1456, 1694, 2136, 2482
XceI	RCATGY	6 cut(s)	581, 739, 1351, 1673, 2645, 2738
XmnI	GAANNNNTTC	1 cut(s)	652
XspI	CTAG	4 cut(s)	518, 1271, 2198, 2292
ZraI	GACGTC	1 cut(s)	382
ZrmI	AGTACT	1 cut(s)	626
Zsp2I	ATGCAT	1 cut(s)	2158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.