Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0009188

Physical Location & Seq

Genomic Coordinates Reverse (-)

7003072 .. 7016888

Gene Structure 13817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022663

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGGTAGAATTGGGATTGAGCAAACCGGTGAGTGTGAGCTTCAGAATACCATATTACACACAGTGGGGCCAAAGCCTGGTGGTGTGCGGCTCCGAACCGGTGCTTGGTTCATGGAATGTCAAGAGGGGTCTGCTGTTGACTCCTGTTCACCAGGGTGATGAGCTCATATGGTTTGGCACCATTTCTGTCCCCAAAGGGTTTGGATCGTCGTGCGAGTACAGTTACTACGTTGTGGATGATGATCGAAACGTTCTTCGATCGGAGATGGGAAACAAGCGTCGGCTTCTTTTGCCTCAGGCCATTCAGGAAGGAGACCTGCTTCACTTCCATGACTTTTGGCAGGTTGGTGCCGATGCAATTCCTTTCAGAAGTGCCTTCAAGGACGTCATCTTTCGTAGAGAGCAGAGTTTGGAGGTTGAGAAACCTCTAGGAGTCATCCAAAATTCACTGCAGAATCATGATTCTGTTCTCGTCCGTTTCAAAATTTGCTGTCCAAATATAGAAGAAGGAACGTCGATTTACATTACTGGTAGTACCTCAAAGTTGGGGAATTGGAAGGCACAGGATGGGCTTAAACTGAGCTATTCTGGTGAATCTACTTGGCATGGAGACTGTTACTGTAAATATAGCAAAGGAGGAAACATTTCTTCTGAAACTGGTCCAAACCGCGAAATTGCTCTCGACTCGTTAAGTACAGAACCAAGATACCTTTTCCGTTCAGATGGCATGTTGCGAGAAATGCCCTGGCGGGGGGCTGGTGTTGCAATCCCAATGTTCTCTGTTAGATCAGAAACTGATCTTGGTGTTGGAGAGTTTCTTGACCTGAAATTACTTGTTGACTGGGCTGCAGAGTCTGGCTTTCATTTAGTTCAGCTTTTACCCATTAATGATACATCCGTGTATGGAATGTGGTGGGATTCATATCCTTACAGTTCACTTTCTGTATATGCATTGCATCCTTTATACTTGAGAGTACAGGCACTTTCAGAAAACATCCCCAAGGATATCAAGGTAGAGATTCAGAAGGCAAAAGAAGAATTGGAGGGAAAGGATGTTGATTACGAGGCTACCTTGACCACTAAACTCTCAATTGCAAAGAAAATATTTGCCCAAGAGAAGGATAAGATTCTCAGCTCCAATTCGTTACAGAATTTTTTCTCTGAGAATCAGGAATGGTTAAAACCCTATGCGGCCTTCTGTTTTTTGCGGGACTTCTTTGAAACATCAGATCACAGTCAATGGGGTCGGTTTTCCCAGTTTTCAATAGAAAAGCTAGAGAAACTCATCTCTAAAGACAGCATTCACTATCGTGAAATTTGCTTCCATTATTACATCCAATTTCATTTGCATATGCAGCTGTCGGAAGCTGCAGAATATGCAAGAAAGAAAGGAGTAGTATTGAAAGGAGACTTGCCTATTGGTGTCGGTAGAGACAGTGTAGATACATGGGTCTATCCAAATCTGTTTCGCATGAACACATCTACTGGAGCTCCTCCGGATTATTTTGATAAAAATGGGCAGAACTGGGGTTTCCCTACTTACAATTGGGATGAGATGGCCAAAGACAATTATGCCTGGTGGCGTGCTCGATTGACACAGATGGCAAAATATTTTACAGCTTACCGGATTGACCATATATTGGGGTTCTTTCGTATCTGGGAACTCCCAGAGCATGCCATTACTGGTCTCGTTGGAAAATTTCGTCCTTCCATTCCTCTGAGTCAGGAAGAACTTGAAAGAGAGAGCATATGGGACTTTGATCGCTTGGCTCATCCATATATCCCACAGGAAATTTTACAGATTAAGTTTGGAGATTCATGGACTTTTATTGCTTCGAGTTTTCTGAATGAATATCAGAAGAACCGCTATGAGTTTAAGGAAGACTGCAACACAGAGAAAAAAATTGCTTCCAAGCTGAAGTCATTTTCAGGAAGATTGTTGTTGCAGAATGAAGACCACATTAGGCATGAGCTCTTTGATATTTTACAGAATATAGTTCTGATAAGAGATCCAGAAAATCCAAGGAACTTCTATCCTCGCTTCAATCTCGAAGAGACTTCAAGTTTTAAGGATTTGGATGATCACTGCAAGAATGTACTGAAACGGTTATACTATGATTACTACTTCCATCGACAAGAAAATCTTTGGCGTCAAAATGCATTGAAGACACTGCCTGCTCTGCTGAACTCGTCAGATATGCTAGCTTGTGGGGAAGATCTGGGGCTTATTCCTTCTTGTGTTTATCCTGTGATGCAAGAACTGGGATTAATAGGTTTACGCATTCAACGTATGCCTAGTGAGCCTGATCTGGAGTTTGGTATCCCTTCTCAATACAATTATATGACAGTGTGTGCTCCATCATGCCATGACTGTTCAACATTGCGTGCTTGGTGGGAAGAAGATGAAGAAAGGAGGCAGCGATTTTTTAGCAGTATGGTGGGATCTGACTTGTTGCCACCCAGTTGCTGTGTTCCAGAGATTGCAAATTTCATCTTAAGGCAACATTTTGAAGCTCCATCAATGTGGGCAATTTTTCCTCTTCAGGATTTGTTGATTTTGAAAGAAGAATACACAACACGCCCTGCAAGAGAGGAGACAATCAATGACCCGACAAATCCGAAGCATTATTGGAGATACCGTGTACATGTGACATTGGAGGCTTTGATCAAGGATAAAGAGCTCACATCGATCATCAAAGACCTCGTGCGTGGAAGTGGAAGATCACATCCGGGGAAACATGCTGAGAAACAAGTGATTCAGGGATCACCTGTTGCAACCACAGAGAAGAAGCAGATTGCCAGCAGCAAAGAAAAGGCTCAAGAGGCAACTCCACTGAACGGAGTACCGCGGAAGGAGGCTGTTGCTGTTCCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0010297 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0030246 GO:0030247 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

CBM_20 Glyco_hydro_77

Protein Analysis

959

Amino Acids

110.63

Weight (kDa)

5.89

Isoelectric Point (pI)

43.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	10 - 102	3.6e-19	Starch binding domain
CBM_20	PF00686	156 - 204	1.1e-07	Starch binding domain
Glyco_hydro_77	PF02446	256 - 883	2.4e-144	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40840
fragaria_vesca	FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260
malus_domestica	MD00G1025200.v1.1
prunus_persica	Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1
pyrus_communis	pycom12g09050
rosa_chinensis	RchiOBHm_Chr3g0494731
rosa_laevigata	RLG00000022663
rosa_multiflora	Rmu_sc0000198.1_g000016
rosa_roxburghii	Rroxscaffold_6G00390950
rosa_rugosa	Rorug03G0268100
rosa_samantha	Rh3AG316600 Rh3BG351900 Rh3CG349000 Rh3DG351500
rosa_wichuraiana	Rw3G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	387
Acc36I	ACCTGC	2 cut(s)	324, 331
AccB1I	GGYRCC	2 cut(s)	176, 347
AccB7I	CCANNNNNTGG	2 cut(s)	76, 1639
AccII	CGCG	2 cut(s)	669, 2848
AccIII	TCCGGA	1 cut(s)	1495
AciI	CCGC	8 cut(s)	87, 667, 748, 1190, 1207, 1864, 2846, 2848
AclI	AACGTT	1 cut(s)	249
AclWI	GGATC	4 cut(s)	211, 2003, 2449, 2770
AcoI	YGGCCR	1 cut(s)	1557
AcsI	RAATTY	7 cut(s)	442, 483, 1150, 1314, 1697, 1791, 2485
AcuI	CTGAAG	3 cut(s)	25, 1937, 2525
AcyI	GRCGYC	2 cut(s)	384, 2149
AdeI	CACNNNGTG	1 cut(s)	63
AfaI	GTAC	7 cut(s)	218, 535, 694, 975, 2097, 2643, 2844
AfiI	CCNNNNNNNGG	7 cut(s)	76, 104, 749, 750, 1117, 1639, 2837
AflII	CTTAAG	1 cut(s)	2494
AflIII	ACRYGT	1 cut(s)	2644
AgeI	ACCGGT	2 cut(s)	25, 97
AjnI	CCWGG	4 cut(s)	75, 150, 743, 1574
AjuI	GAANNNNNNNTTGG	4 cut(s)	87, 119, 783, 815
AleI	CACNNNNGTG	2 cut(s)	153, 1308
Alw21I	GWGCWC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
Alw26I	GTCTC	7 cut(s)	306, 603, 1401, 1425, 1691, 2048, 2588
AlwI	GGATC	4 cut(s)	211, 2003, 2449, 2770
AlwNI	CAGNNNCTG	3 cut(s)	794, 854, 2466
Aor13HI	TCCGGA	1 cut(s)	1495
AoxI	GGCC	4 cut(s)	67, 297, 1191, 1557
ApeKI	GCWGC	5 cut(s)	845, 1354, 1367, 2416, 2802
ApoI	RAATTY	7 cut(s)	442, 483, 1150, 1314, 1697, 1791, 2485
AseI	ATTAAT	2 cut(s)	885, 2267
AsiGI	ACCGGT	2 cut(s)	25, 97
Asp700I	GAANNNNTTC	2 cut(s)	643, 1154
AspS9I	GGNCC	2 cut(s)	67, 659
AsuC2I	CCSGG	1 cut(s)	2730
AsuHPI	GGTGA	5 cut(s)	40, 140, 167, 602, 2757
AsuNHI	GCTAGC	1 cut(s)	2200
AvaII	GGWCC	1 cut(s)	659
AxyI	CCTNAGG	1 cut(s)	294
BaeI	ACNNNNGTAYC	4 cut(s)	882, 915, 1434, 1467
BalI	TGGCCA	1 cut(s)	1559
BanI	GGYRCC	2 cut(s)	176, 347
BanII	GRGCYC	4 cut(s)	165, 1492, 1974, 2682
BauI	CACGAG	1 cut(s)	2702
BbsI	GAAGAC	3 cut(s)	1887, 1959, 2171
Bbv12I	GWGCWC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
BbvI	GCAGC	5 cut(s)	832, 1354, 1366, 2428, 2814
BccI	CCATC	8 cut(s)	259, 560, 716, 1549, 1594, 2136, 2365, 2524
BciT130I	CCWGG	4 cut(s)	77, 152, 745, 1576
BciVI	GTATCC	1 cut(s)	2330
BclI	TGATCA	2 cut(s)	2080, 2664
BcnI	CCSGG	1 cut(s)	2730
BcoDI	GTCTC	7 cut(s)	306, 603, 1401, 1425, 1691, 2048, 2588
BfaI	CTAG	4 cut(s)	428, 1274, 2201, 2295
BfmI	CTRYAG	3 cut(s)	449, 846, 1368
BfrI	CTTAAG	1 cut(s)	2494
BfuAI	ACCTGC	2 cut(s)	324, 331
BfuI	GTATCC	1 cut(s)	2330
BglII	AGATCT	1 cut(s)	2215
BisI	GCNGC	7 cut(s)	88, 846, 1191, 1355, 1368, 2417, 2803
BlsI	GCNGC	7 cut(s)	89, 847, 1192, 1356, 1369, 2418, 2804
Bme1390I	CCNGG	5 cut(s)	77, 152, 745, 1576, 2730
Bme18I	GGWCC	1 cut(s)	659
BmgT120I	GGNCC	2 cut(s)	67, 659
BmiI	GGNNCC	4 cut(s)	68, 91, 178, 349
BmrFI	CCNGG	5 cut(s)	77, 152, 745, 1576, 2730
BmrI	ACTGGG	5 cut(s)	850, 1249, 1534, 2270, 2454
BmsI	GCATC	3 cut(s)	343, 964, 2241
BmtI	GCTAGC	1 cut(s)	2204
BmuI	ACTGGG	5 cut(s)	850, 1249, 1534, 2270, 2454
BpiI	GAAGAC	3 cut(s)	1887, 1959, 2171
BpmI	CTGGAG	2 cut(s)	1506, 2330
BpuEI	CTTGAG	2 cut(s)	988, 2802
BpuMI	CCSGG	1 cut(s)	2730
Bsa29I	ATCGAT	1 cut(s)	2687
BsaBI	GATNNNNATC	2 cut(s)	240, 921
BsaHI	GRCGYC	2 cut(s)	384, 2149
BsaI	GGTCTC	2 cut(s)	306, 1691
BsaJI	CCNNGG	6 cut(s)	151, 743, 999, 2021, 2729, 2846
BsaWI	WCCGGW	4 cut(s)	25, 97, 1495, 1623
BsaXI	ACNNNNNCTCC	4 cut(s)	1474, 1504, 2623, 2653
Bsc4I	CCNNNNNNNGG	7 cut(s)	76, 104, 749, 750, 1117, 1639, 2837
Bse118I	RCCGGY	2 cut(s)	25, 97
Bse1I	ACTGG	9 cut(s)	532, 661, 845, 1255, 1489, 1529, 1687, 2265, 2460
Bse21I	CCTNAGG	1 cut(s)	294
Bse3DI	GCAATG	2 cut(s)	950, 2378
Bse8I	GATNNNNATC	2 cut(s)	240, 921
BseAI	TCCGGA	1 cut(s)	1495
BseBI	CCWGG	4 cut(s)	77, 152, 745, 1576
BseCI	ATCGAT	1 cut(s)	2687
BseDI	CCNNGG	6 cut(s)	151, 743, 999, 2021, 2729, 2846
BseJI	GATNNNNATC	2 cut(s)	240, 921
BseLI	CCNNNNNNNGG	7 cut(s)	76, 104, 749, 750, 1117, 1639, 2837
BseMI	GCAATG	2 cut(s)	950, 2378
BseMII	CTCAG	6 cut(s)	308, 569, 1144, 1152, 1709, 2733
BseNI	ACTGG	9 cut(s)	532, 661, 845, 1255, 1489, 1529, 1687, 2265, 2460
BseRI	GAGGAG	2 cut(s)	1482, 2606
BseXI	GCAGC	5 cut(s)	832, 1354, 1366, 2428, 2814
Bsh1236I	CGCG	2 cut(s)	669, 2848
Bsh1285I	CGRYCG	1 cut(s)	260
BshFI	GGCC	4 cut(s)	69, 299, 1193, 1559
BshNI	GGYRCC	2 cut(s)	176, 347
BshTI	ACCGGT	2 cut(s)	25, 97
BshVI	ATCGAT	1 cut(s)	2687
BsiEI	CGRYCG	1 cut(s)	260
BsiHKAI	GWGCWC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
BsiSI	CCGG	5 cut(s)	26, 98, 1496, 1624, 2729
BslFI	GGGAC	3 cut(s)	173, 1223, 1766
BslI	CCNNNNNNNGG	7 cut(s)	76, 104, 749, 750, 1117, 1639, 2837
BsmAI	GTCTC	7 cut(s)	306, 603, 1401, 1425, 1691, 2048, 2588
BsmFI	GGGAC	3 cut(s)	173, 1223, 1766
BsmI	GAATGC	2 cut(s)	1299, 2280
BsnI	GGCC	4 cut(s)	69, 299, 1193, 1559
Bso31I	GGTCTC	2 cut(s)	306, 1691
Bsp1286I	GDGCHC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
Bsp13I	TCCGGA	1 cut(s)	1495
Bsp1407I	TGTACA	1 cut(s)	2641
BspACI	CCGC	8 cut(s)	87, 667, 748, 1190, 1207, 1864, 2846, 2848
BspANI	GGCC	4 cut(s)	69, 299, 1193, 1559
BspCNI	CTCAG	6 cut(s)	307, 570, 1143, 1153, 1710, 2734
BspDI	ATCGAT	1 cut(s)	2687
BspEI	TCCGGA	1 cut(s)	1495
BspFNI	CGCG	2 cut(s)	669, 2848
BspHI	TCATGA	1 cut(s)	457
BspLI	GGNNCC	4 cut(s)	68, 91, 178, 349
BspMAI	CTGCAG	3 cut(s)	453, 850, 1372
BspMI	ACCTGC	2 cut(s)	324, 331
BspOI	GCTAGC	1 cut(s)	2204
BspPI	GGATC	4 cut(s)	211, 2003, 2449, 2770
BspT107I	GGYRCC	2 cut(s)	176, 347
BspTI	CTTAAG	1 cut(s)	2494
BspTNI	GGTCTC	2 cut(s)	306, 1691
BsrDI	GCAATG	2 cut(s)	950, 2378
BsrFI	RCCGGY	2 cut(s)	25, 97
BsrGI	TGTACA	1 cut(s)	2641
BsrI	ACTGG	9 cut(s)	532, 661, 845, 1255, 1489, 1529, 1687, 2265, 2460
BssAI	RCCGGY	2 cut(s)	25, 97
BssECI	CCNNGG	6 cut(s)	151, 743, 999, 2021, 2729, 2846
BssNI	GRCGYC	2 cut(s)	384, 2149
BssSI	CACGAG	1 cut(s)	2702
BssT1I	CCWWGG	2 cut(s)	999, 2021
Bst2BI	CACGAG	1 cut(s)	2702
Bst2UI	CCWGG	4 cut(s)	77, 152, 745, 1576
Bst6I	CTCTTC	2 cut(s)	2046, 2544
BstACI	GRCGYC	2 cut(s)	384, 2149
BstAFI	CTTAAG	1 cut(s)	2494
BstAPI	GCANNNNNTGC	1 cut(s)	1376
BstAUI	TGTACA	1 cut(s)	2641
BstC8I	GCNNGC	6 cut(s)	1584, 1674, 2175, 2202, 2385, 2800
BstDEI	CTNAG	6 cut(s)	294, 578, 1130, 1161, 1718, 2742
BstDSI	CCRYGG	1 cut(s)	2846
BstFNI	CGCG	2 cut(s)	669, 2848
BstMAI	GTCTC	7 cut(s)	306, 603, 1401, 1425, 1691, 2048, 2588
BstMCI	CGRYCG	1 cut(s)	260
BstMWI	GCNNNNNNNGC	8 cut(s)	289, 739, 761, 1354, 1376, 2179, 2299, 2822
BstNI	CCWGG	4 cut(s)	77, 152, 745, 1576
BstNSI	RCATGY	4 cut(s)	730, 1676, 2648, 2741
BstSCI	CCNGG	5 cut(s)	75, 150, 743, 1574, 2728
BstSFI	CTRYAG	3 cut(s)	449, 846, 1368
BstUI	CGCG	2 cut(s)	669, 2848
BstV1I	GCAGC	5 cut(s)	832, 1354, 1366, 2428, 2814
BstV2I	GAAGAC	3 cut(s)	1887, 1959, 2171
BstX2I	RGATCY	3 cut(s)	2008, 2215, 2441
BstXI	CCANNNNNNTGG	1 cut(s)	2523
BstYI	RGATCY	3 cut(s)	2008, 2215, 2441
Bsu15I	ATCGAT	1 cut(s)	2687
Bsu36I	CCTNAGG	1 cut(s)	294
BsuI	GTATCC	1 cut(s)	2330
BsuRI	GGCC	4 cut(s)	69, 299, 1193, 1559
BsuTUI	ATCGAT	1 cut(s)	2687
BtgI	CCRYGG	1 cut(s)	2846
BtsI	GCAGTG	3 cut(s)	446, 2083, 2168
BtsIMutI	CAGTG	7 cut(s)	68, 446, 1441, 2083, 2168, 2352, 2831
BveI	ACCTGC	2 cut(s)	324, 331
Cac8I	GCNNGC	6 cut(s)	1584, 1674, 2175, 2202, 2385, 2800
CaiI	CAGNNNCTG	3 cut(s)	794, 854, 2466
CciI	TCATGA	1 cut(s)	457
Cfr10I	RCCGGY	2 cut(s)	25, 97
Cfr13I	GGNCC	2 cut(s)	67, 659
Cfr42I	CCGCGG	1 cut(s)	2849
ClaI	ATCGAT	1 cut(s)	2687
CseI	GACGC	2 cut(s)	266, 2138
Csp6I	GTAC	7 cut(s)	217, 534, 693, 974, 2096, 2642, 2843
CspAI	ACCGGT	2 cut(s)	25, 97
CviQI	GTAC	7 cut(s)	217, 534, 693, 974, 2096, 2642, 2843
DdeI	CTNAG	6 cut(s)	294, 578, 1130, 1161, 1718, 2742
DraIII	CACNNNGTG	1 cut(s)	63
EaeI	YGGCCR	1 cut(s)	1557
Eam1104I	CTCTTC	2 cut(s)	2046, 2544
EarI	CTCTTC	2 cut(s)	2046, 2544
Ecl136II	GAGCTC	4 cut(s)	163, 1490, 1972, 2680
Eco130I	CCWWGG	2 cut(s)	999, 2021
Eco24I	GRGCYC	4 cut(s)	165, 1492, 1974, 2682
Eco31I	GGTCTC	2 cut(s)	306, 1691
Eco32I	GATATC	1 cut(s)	1006
Eco47I	GGWCC	1 cut(s)	659
Eco53kI	GAGCTC	4 cut(s)	163, 1490, 1972, 2680
Eco57I	CTGAAG	3 cut(s)	25, 1937, 2525
Eco81I	CCTNAGG	1 cut(s)	294
EcoICRI	GAGCTC	4 cut(s)	163, 1490, 1972, 2680
EcoRII	CCWGG	4 cut(s)	75, 150, 743, 1574
EcoRV	GATATC	1 cut(s)	1006
EcoT14I	CCWWGG	2 cut(s)	999, 2021
EcoT22I	ATGCAT	2 cut(s)	952, 2161
EcoT38I	GRGCYC	4 cut(s)	165, 1492, 1974, 2682
ErhI	CCWWGG	2 cut(s)	999, 2021
FalI	AAGNNNNNCTT	2 cut(s)	2127, 2159
FaqI	GGGAC	3 cut(s)	173, 1223, 1766
FauI	CCCGC	2 cut(s)	741, 1200
FauNDI	CATATG	3 cut(s)	167, 1350, 1748
FbaI	TGATCA	2 cut(s)	2080, 2664
Fnu4HI	GCNGC	7 cut(s)	88, 846, 1191, 1355, 1368, 2417, 2803
FriOI	GRGCYC	4 cut(s)	165, 1492, 1974, 2682
Fsp4HI	GCNGC	7 cut(s)	88, 846, 1191, 1355, 1368, 2417, 2803
FspBI	CTAG	4 cut(s)	428, 1274, 2201, 2295
GluI	GCNGC	7 cut(s)	88, 846, 1191, 1355, 1368, 2417, 2803
GsuI	CTGGAG	2 cut(s)	1506, 2330
HaeIII	GGCC	4 cut(s)	69, 299, 1193, 1559
HapII	CCGG	5 cut(s)	26, 98, 1496, 1624, 2729
HgaI	GACGC	2 cut(s)	266, 2138
Hin1I	GRCGYC	2 cut(s)	384, 2149
HincII	GTYRAC	2 cut(s)	138, 838
HindII	GTYRAC	2 cut(s)	138, 838
HpaII	CCGG	5 cut(s)	26, 98, 1496, 1624, 2729
HphI	GGTGA	5 cut(s)	40, 140, 167, 602, 2757
Hpy166II	GTNNAC	6 cut(s)	138, 148, 838, 935, 2276, 2642
Hpy8I	GTNNAC	6 cut(s)	138, 148, 838, 935, 2276, 2642
Hpy99I	CGWCG	3 cut(s)	211, 282, 517
HpyCH4IV	ACGT	5 cut(s)	228, 249, 384, 512, 2287
HpyF10VI	GCNNNNNNNGC	8 cut(s)	289, 739, 761, 1354, 1376, 2179, 2299, 2822
HpyF3I	CTNAG	6 cut(s)	294, 578, 1130, 1161, 1718, 2742
HpySE526I	ACGT	5 cut(s)	228, 249, 384, 512, 2287
Hsp92I	GRCGYC	2 cut(s)	384, 2149
Kpn2I	TCCGGA	1 cut(s)	1495
Ksp22I	TGATCA	2 cut(s)	2080, 2664
KspI	CCGCGG	1 cut(s)	2849
LmnI	GCTCC	6 cut(s)	95, 1139, 1487, 1495, 2359, 2518
Lsp1109I	GCAGC	5 cut(s)	832, 1354, 1366, 2428, 2814
LweI	GCATC	3 cut(s)	343, 964, 2241
MaeI	CTAG	4 cut(s)	428, 1274, 2201, 2295
MaeII	ACGT	5 cut(s)	228, 249, 384, 512, 2287
MaeIII	GTNAC	4 cut(s)	221, 614, 1143, 2647
MfeI	CAATTG	2 cut(s)	1089, 1543
MflI	RGATCY	3 cut(s)	2008, 2215, 2441
MhlI	GDGCHC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
MlsI	TGGCCA	1 cut(s)	1559
MluNI	TGGCCA	1 cut(s)	1559
MlyI	GAGTC	5 cut(s)	133, 441, 677, 860, 1729
MmeI	TCCRAC	3 cut(s)	787, 1341, 1672
Mox20I	TGGCCA	1 cut(s)	1559
Mph1103I	ATGCAT	2 cut(s)	952, 2161
MroI	TCCGGA	1 cut(s)	1495
MroXI	GAANNNNTTC	2 cut(s)	643, 1154
MscI	TGGCCA	1 cut(s)	1559
MslI	CAYNNNNRTG	4 cut(s)	153, 327, 1308, 2521
Msp20I	TGGCCA	1 cut(s)	1559
MspA1I	CMGCKG	2 cut(s)	1357, 2848
MspCI	CTTAAG	1 cut(s)	2494
MspI	CCGG	5 cut(s)	26, 98, 1496, 1624, 2729
MspR9I	CCNGG	5 cut(s)	77, 152, 745, 1576, 2730
MunI	CAATTG	2 cut(s)	1089, 1543
Mva1269I	GAATGC	2 cut(s)	1299, 2280
MvaI	CCWGG	4 cut(s)	77, 152, 745, 1576
MvnI	CGCG	2 cut(s)	669, 2848
MwoI	GCNNNNNNNGC	8 cut(s)	289, 739, 761, 1354, 1376, 2179, 2299, 2822
NciI	CCSGG	1 cut(s)	2730
NdeI	CATATG	3 cut(s)	167, 1350, 1748
NheI	GCTAGC	1 cut(s)	2200
NlaIV	GGNNCC	4 cut(s)	68, 91, 178, 349
NmuCI	GTSAC	1 cut(s)	2647
NsiI	ATGCAT	2 cut(s)	952, 2161
NspI	RCATGY	4 cut(s)	730, 1676, 2648, 2741
OliI	CACNNNNGTG	2 cut(s)	153, 1308
PaeI	GCATGC	1 cut(s)	1676
PagI	TCATGA	1 cut(s)	457
PciI	ACATGT	1 cut(s)	2644
PcsI	WCGNNNNNNNCGW	1 cut(s)	2284
PctI	GAATGC	2 cut(s)	1299, 2280
PdmI	GAANNNNTTC	2 cut(s)	643, 1154
PfeI	GAWTC	9 cut(s)	454, 461, 593, 917, 1018, 1126, 1165, 1814, 2755
PflMI	CCANNNNNTGG	2 cut(s)	76, 1639
PinAI	ACCGGT	2 cut(s)	25, 97
PkrI	GCNGC	7 cut(s)	89, 847, 1192, 1356, 1369, 2418, 2804
Ple19I	CGATCG	1 cut(s)	260
PleI	GAGTC	5 cut(s)	133, 440, 677, 859, 1728
PpsI	GAGTC	5 cut(s)	133, 440, 677, 859, 1728
PscI	ACATGT	1 cut(s)	2644
PshBI	ATTAAT	2 cut(s)	885, 2267
Psp124BI	GAGCTC	4 cut(s)	165, 1492, 1974, 2682
Psp1406I	AACGTT	1 cut(s)	249
Psp6I	CCWGG	4 cut(s)	75, 150, 743, 1574
PspGI	CCWGG	4 cut(s)	75, 150, 743, 1574
PspN4I	GGNNCC	4 cut(s)	68, 91, 178, 349
PspPI	GGNCC	2 cut(s)	67, 659
PstI	CTGCAG	3 cut(s)	453, 850, 1372
PstNI	CAGNNNCTG	3 cut(s)	794, 854, 2466
PsuI	RGATCY	3 cut(s)	2008, 2215, 2441
PvuI	CGATCG	1 cut(s)	260
PvuII	CAGCTG	1 cut(s)	1357
RsaI	GTAC	7 cut(s)	218, 535, 694, 975, 2097, 2643, 2844
RsaNI	GTAC	7 cut(s)	217, 534, 693, 974, 2096, 2642, 2843
RseI	CAYNNNNRTG	4 cut(s)	153, 327, 1308, 2521
SacI	GAGCTC	4 cut(s)	165, 1492, 1974, 2682
SacII	CCGCGG	1 cut(s)	2849
SatI	GCNGC	7 cut(s)	88, 846, 1191, 1355, 1368, 2417, 2803
Sau96I	GGNCC	2 cut(s)	67, 659
SchI	GAGTC	5 cut(s)	133, 441, 677, 860, 1729
ScrFI	CCNGG	5 cut(s)	77, 152, 745, 1576, 2730
SduI	GDGCHC	6 cut(s)	165, 1492, 1588, 1974, 2356, 2682
SfaNI	GCATC	3 cut(s)	343, 964, 2241
SfcI	CTRYAG	3 cut(s)	449, 846, 1368
Sfr303I	CCGCGG	1 cut(s)	2849
SgrBI	CCGCGG	1 cut(s)	2849
SinI	GGWCC	1 cut(s)	659
SmiMI	CAYNNNNRTG	4 cut(s)	153, 327, 1308, 2521
SmlI	CTYRAG	3 cut(s)	967, 2494, 2817
SmoI	CTYRAG	3 cut(s)	967, 2494, 2817
SphI	GCATGC	1 cut(s)	1676
SsiI	CCGC	8 cut(s)	87, 667, 748, 1190, 1207, 1864, 2846, 2848
SspI	AATATT	2 cut(s)	1104, 1610
SspMI	CTAG	4 cut(s)	428, 1274, 2201, 2295
SstI	GAGCTC	4 cut(s)	165, 1492, 1974, 2682
StyD4I	CCNGG	5 cut(s)	75, 150, 743, 1574, 2728
StyI	CCWWGG	2 cut(s)	999, 2021
TaiI	ACGT	5 cut(s)	231, 252, 387, 515, 2290
TaqI	TCGA	9 cut(s)	244, 256, 515, 681, 1588, 1835, 2049, 2131, 2687
TatI	WGTACW	5 cut(s)	216, 692, 973, 2095, 2641
TauI	GCSGC	2 cut(s)	90, 1193
TfiI	GAWTC	9 cut(s)	454, 461, 593, 917, 1018, 1126, 1165, 1814, 2755
TscAI	CASTG	7 cut(s)	68, 453, 1441, 2090, 2175, 2352, 2838
TseFI	GTSAC	1 cut(s)	2647
TseI	GCWGC	5 cut(s)	845, 1354, 1367, 2416, 2802
Tsp45I	GTSAC	1 cut(s)	2647
TspGWI	ACGGA	4 cut(s)	464, 704, 886, 2853
TspRI	CASTG	7 cut(s)	68, 453, 1441, 2090, 2175, 2352, 2838
Van91I	CCANNNNNTGG	2 cut(s)	76, 1639
Vha464I	CTTAAG	1 cut(s)	2494
VpaK11BI	GGWCC	1 cut(s)	659
VspI	ATTAAT	2 cut(s)	885, 2267
XapI	RAATTY	7 cut(s)	442, 483, 1150, 1314, 1697, 1791, 2485
XceI	RCATGY	4 cut(s)	730, 1676, 2648, 2741
XmnI	GAANNNNTTC	2 cut(s)	643, 1154
XspI	CTAG	4 cut(s)	428, 1274, 2201, 2295
ZraI	GACGTC	1 cut(s)	385
Zsp2I	ATGCAT	2 cut(s)	952, 2161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.