Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0009188

Physical Location & Seq

Genomic Coordinates Forward (+)

42033014 .. 42048083

Gene Structure 15070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG351500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2943 bp

ATGGTAGAATTGGGATTGAGCAGGGCCAGCAAACCGGTGAGTGTGAGCTTCAGAATACCATATTACACACAGTGGGGCCAAAGCCTGGTGGTGTGCGGCTCCGAACCGGTGCTTGGTTCTTGGAATGTCAAGAGGGGTCTGCAGTTGAGTCCTGTTCACCAGGGTGATGAGCTCATATGGTTTGGCACCATTTCTGTCCCCAAAGGGTTTGGATCGTGCGAGTACAGTTACTACGTTGTGGATGATGATCGAAATGTTCTCCGATGGGAGATGGGAAGCAAGCGTCGGCTTCTTTTGCCTCAGGCCATTCAGGAAGGAGAGCTGCTTCACTTCCATGACTTTTGGCAGGTTGGTGCCGATGCAATTCCGTTCAGAAGTGCCTTCAAGGACGTCATCTTTCGTGGAGACTGGAGTTTGGAGGTTGACAAACCTCTAGGAGTCATCCAAAATTCATTGCAGAATGATGATTCTGTTCTCGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGGAACATCGATTTACATTACTGGTAGTACCTCAAAGTTGGGGAATTGGAAGGCACAGGATGGGCTTAAACTGAGCTATTCTGGTGAATCTACTTGGCATAAAGACTGTGTCTTGCTGAAAGGGGACTTCCCGATAAAATATCCTTTTGGAGCCTTTAAATCTTTAAAATACTGTAAATATAGCAAAGGAGGAAACATTTCTTCTGAAACTGGTCCAAACCGCGAAATTGCTCTCGACTCGTTAAGTACTGAACCAAGATACCTTTTCCGTTCAGATGGCATGTTGCGAGAAATGCCTTGGCGGGGGGCTGGTGTTGCAATCCCAATGTTCTCTATTAGATCAGAAACTGATCTTGGTGTTGGAGAGTTTCTTGACCTGAAATTACTTGTTGACTGGGCTGCAGAGTCTGGCTTTCATTTAGTTCAGCTTTTACCCATTAATGATACATCCGTGTATGGAATGTGGTGGGATTCATATCCTTACAGCTCACTTTCTGTATATGCATTGCATCCTTTATACTTGAGAGTACAGGCACTTTCAGAAAACATCCCCAAGGATATCAAGGTAGAGATTCAGAAGGCAAAAGAAGAATTGGAGGGAAAGGATGTTGATTACGAGGCTACCTTGACCACTAAACTCTCAATTGCAAAGAAAATATTTGCCCAAGAGAAGGATAAGATTCTCAGCTCCAATTCGTTCCAGAAATTTTTCTCTGAGAATCAGGAATGGTTGAAACCCTATGCGGCCTTCTGTTTTTTGCGGGACTTCTTTGAAACATCAGATCACAGTCAATGGGGTCGGTTTTCCCAGTTTTCAATAGAAAAGCTAGAGAAACTCATCTCTAAAGACAGCATTCACTATCGTGAAATTTGCTTCCATTATTACATCCAATTTCATTTGCATATGCAGCTGTCGGAAGCTGCAGAATATGCTAGAAAGAAAGGAGTAGTATTGAAAGGAGACTTGCCTATTGGTGTCGGTAGAGACAGTGTAGATACGTGGGTCTATCCAAATCTGTTTCGCATGAACACATCTACTGGAGCTCCTCCAGATTATTTTGATAAAAATGGGCAGAACTGGGGTTTCCCTACTTACAATTGGGACGAGATGGCCAAAGACAATTATGCCTGGTGGCGTGCTCGATTGACACAGATGGCAAAATACTTTACAGCTTACCGGATTGACCATATATTGGGGTTCTTTCGTATCTGGGAACTCCCAGAGCATGCCATTACTGGTCTCGTTGGAAAATTTCGTCCTTCCATTCCTCTAAGTCAGGAAGAACTTGAAAGAGAGGGCATATGGGACTTTGATCGCTTGGCTCGTCCATATATCCCACAGGAAATTTTACAGATTAAGTTTGGAGATTCATGGACTTTTATTGCTTCGAGTTTTCTGAATGAATATCAGAAGAACCGCTATGAGTTTAAGGAAGACTGCAACACAGAGAAAAAAATTGCTTCCAAGCTGAAGTCATTTTCAGGAAGATCGTTGTTGCAGAATGAAGACCACATTAGGCATGAGCTCTTTGATATTTTACAGAATATAGTTCTGATAAGGGATCCAGAAAATCCAAGGAACTTCTATCCTCGCTTCAATCTCGAAGAGACTTCAAGTTTTATGGATTTGGATGATCACTGCAAGAATGTACTGAAACGGTTATACTATGATTACTACTTCCATCGACAAGAAAATCTTTGGCGTCAAAATGCATTGAAGACACTGCCTGCTCTGCTGAACTCATCGGATATGCTAGCTTGTGGGGAAGATCTGGGGCTTATTCCTTCTTGTGTTTATCCTGTGATGCAAGAACTTGGATTAATAGGTTTACGCATTCAACGTATGCCTAGTGAGCCTGATCTGGAGTTTGGTATCCCTTCTCAATACAATTATATGACAGTGTGTGCTCCATCATGCCATGACTGTTCAACGTTGCGTGCTTGGTGGGAAGAAGATGAAGAAAGGAGGCAGCGATTTTTTAGCAGTATGGTGGGATCTCACTTGTTGCCACCCAGTCACTGTGTTCCAGAGATTGCAAATTTCATCTTAAGGCAACATTTTGAAGCTCCATCAATGTGGGCAATTTTTCCTCTTCAGGATTTGTTGATTTTGAAAGAAGAATACACAACACGCCCTGCAAAAGAGGAGACGATCAATGACCCGACAAATCCGAAGCATTATTGGAGATACCGTGTACATGTGACATTGGAGGCTTTGATCAAGGATAAAGAGCTCACATCTATCATCAAAGACCTCGTGCGTGGAAGTGGAAGATCACATCCGGGGAAACAAGCTGAGAAACAAGTGATTCAGGAATCACCTGTTGCAACCACAGAGAAGAAGCAGATTGCCAGCAGCAAAGAAAAGGCTCAAGTGGCAACTCCACTGAACGGAGTACCGCGGAAGGAAGCTGTTGCTGTTCCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0010297 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0030246 GO:0030247 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

CBM_20 Glyco_hydro_77

Protein Analysis

980

Amino Acids

113.24

Weight (kDa)

6.09

Isoelectric Point (pI)

42.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	13 - 104	8.1e-22	Starch binding domain
CBM_20	PF00686	158 - 250	1.1e-10	Starch binding domain
Glyco_hydro_77	PF02446	278 - 905	9.8e-146	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40840
fragaria_vesca	FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260
malus_domestica	MD00G1025200.v1.1
prunus_persica	Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1
pyrus_communis	pycom12g09050
rosa_chinensis	RchiOBHm_Chr3g0494731
rosa_laevigata	RLG00000022663
rosa_multiflora	Rmu_sc0000198.1_g000016
rosa_roxburghii	Rroxscaffold_6G00390950
rosa_rugosa	Rorug03G0268100
rosa_samantha	Rh3AG316600 Rh3BG351900 Rh3CG349000 Rh3DG351500
rosa_wichuraiana	Rw3G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	393
Acc36I	ACCTGC	1 cut(s)	337
AccB1I	GGYRCC	2 cut(s)	185, 353
AccB7I	CCANNNNNTGG	2 cut(s)	85, 1705
AccII	CGCG	2 cut(s)	735, 2914
AciI	CCGC	8 cut(s)	96, 733, 814, 1256, 1273, 1930, 2912, 2914
AclI	AACGTT	1 cut(s)	2444
AclWI	GGATC	4 cut(s)	220, 2069, 2082, 2515
AcoI	YGGCCR	1 cut(s)	1623
AcsI	RAATTY	7 cut(s)	448, 489, 1217, 1380, 1763, 1857, 2551
AcuI	CTGAAG	3 cut(s)	34, 2003, 2591
AcyI	GRCGYC	2 cut(s)	390, 2215
AdeI	CACNNNGTG	1 cut(s)	72
AfaI	GTAC	7 cut(s)	224, 541, 760, 1041, 2163, 2709, 2910
AfiI	CCNNNNNNNGG	6 cut(s)	85, 113, 815, 1183, 1705, 2903
AflII	CTTAAG	1 cut(s)	2560
AflIII	ACRYGT	1 cut(s)	2710
AgeI	ACCGGT	2 cut(s)	34, 106
AjnI	CCWGG	3 cut(s)	84, 159, 1640
AjuI	GAANNNNNNNTTGG	4 cut(s)	96, 128, 849, 881
AleI	CACNNNNGTG	2 cut(s)	162, 1374
Alw21I	GWGCWC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
Alw26I	GTCTC	6 cut(s)	399, 1467, 1491, 1757, 2114, 2654
AlwI	GGATC	4 cut(s)	220, 2069, 2082, 2515
AlwNI	CAGNNNCTG	3 cut(s)	860, 920, 2532
AoxI	GGCC	5 cut(s)	24, 76, 303, 1257, 1623
ApeKI	GCWGC	6 cut(s)	322, 911, 1420, 1433, 2482, 2868
ApoI	RAATTY	7 cut(s)	448, 489, 1217, 1380, 1763, 1857, 2551
AseI	ATTAAT	2 cut(s)	951, 2333
AsiGI	ACCGGT	2 cut(s)	34, 106
Asp700I	GAANNNNTTC	2 cut(s)	709, 1220
AspS9I	GGNCC	3 cut(s)	24, 76, 725
AsuC2I	CCSGG	1 cut(s)	2796
AsuHPI	GGTGA	5 cut(s)	49, 149, 176, 608, 2823
AsuNHI	GCTAGC	1 cut(s)	2266
AvaII	GGWCC	1 cut(s)	725
AxyI	CCTNAGG	1 cut(s)	300
BaeI	ACNNNNGTAYC	4 cut(s)	948, 981, 1500, 1533
BalI	TGGCCA	1 cut(s)	1625
BamHI	GGATCC	1 cut(s)	2074
BanI	GGYRCC	2 cut(s)	185, 353
BanII	GRGCYC	4 cut(s)	174, 1558, 2040, 2748
BauI	CACGAG	1 cut(s)	2768
BbsI	GAAGAC	3 cut(s)	1953, 2025, 2237
Bbv12I	GWGCWC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
BbvI	GCAGC	6 cut(s)	309, 898, 1420, 1432, 2494, 2880
BccI	CCATC	9 cut(s)	258, 265, 566, 782, 1615, 1660, 2202, 2431, 2590
BciT130I	CCWGG	3 cut(s)	86, 161, 1642
BciVI	GTATCC	1 cut(s)	2396
BclI	TGATCA	2 cut(s)	2146, 2730
BcnI	CCSGG	1 cut(s)	2796
BcoDI	GTCTC	6 cut(s)	399, 1467, 1491, 1757, 2114, 2654
BfaI	CTAG	5 cut(s)	434, 1340, 1446, 2267, 2361
BfmI	CTRYAG	3 cut(s)	140, 912, 1434
BfrI	CTTAAG	1 cut(s)	2560
BfuAI	ACCTGC	1 cut(s)	337
BfuI	GTATCC	1 cut(s)	2396
BglII	AGATCT	1 cut(s)	2281
BisI	GCNGC	8 cut(s)	97, 323, 912, 1257, 1421, 1434, 2483, 2869
BlsI	GCNGC	8 cut(s)	98, 324, 913, 1258, 1422, 1435, 2484, 2870
BmcAI	AGTACT	1 cut(s)	760
Bme1390I	CCNGG	4 cut(s)	86, 161, 1642, 2796
Bme18I	GGWCC	1 cut(s)	725
BmgT120I	GGNCC	3 cut(s)	24, 76, 725
BmiI	GGNNCC	6 cut(s)	77, 100, 187, 355, 664, 2076
BmrFI	CCNGG	4 cut(s)	86, 161, 1642, 2796
BmrI	ACTGGG	4 cut(s)	916, 1315, 1600, 2520
BmsI	GCATC	3 cut(s)	349, 1030, 2307
BmtI	GCTAGC	1 cut(s)	2270
BmuI	ACTGGG	4 cut(s)	916, 1315, 1600, 2520
BpiI	GAAGAC	3 cut(s)	1953, 2025, 2237
BpmI	CTGGAG	4 cut(s)	430, 1545, 1572, 2396
BpuEI	CTTGAG	2 cut(s)	1054, 2868
BpuMI	CCSGG	1 cut(s)	2796
Bsa29I	ATCGAT	1 cut(s)	521
BsaAI	YACGTR	1 cut(s)	1512
BsaBI	GATNNNNATC	2 cut(s)	246, 987
BsaHI	GRCGYC	2 cut(s)	390, 2215
BsaI	GGTCTC	1 cut(s)	1757
BsaJI	CCNNGG	6 cut(s)	160, 809, 1065, 2087, 2795, 2912
BsaWI	WCCGGW	3 cut(s)	34, 106, 1689
BsaXI	ACNNNNNCTCC	4 cut(s)	1540, 1570, 2689, 2719
Bsc4I	CCNNNNNNNGG	6 cut(s)	85, 113, 815, 1183, 1705, 2903
Bse118I	RCCGGY	2 cut(s)	34, 106
Bse1I	ACTGG	9 cut(s)	413, 538, 727, 911, 1321, 1555, 1595, 1753, 2526
Bse21I	CCTNAGG	1 cut(s)	300
Bse3DI	GCAATG	2 cut(s)	452, 1016
Bse8I	GATNNNNATC	2 cut(s)	246, 987
BseBI	CCWGG	3 cut(s)	86, 161, 1642
BseCI	ATCGAT	1 cut(s)	521
BseDI	CCNNGG	6 cut(s)	160, 809, 1065, 2087, 2795, 2912
BseJI	GATNNNNATC	2 cut(s)	246, 987
BseLI	CCNNNNNNNGG	6 cut(s)	85, 113, 815, 1183, 1705, 2903
BseMI	GCAATG	2 cut(s)	452, 1016
BseMII	CTCAG	5 cut(s)	314, 575, 1210, 1218, 2799
BseNI	ACTGG	9 cut(s)	413, 538, 727, 911, 1321, 1555, 1595, 1753, 2526
BseRI	GAGGAG	2 cut(s)	1548, 2672
BseXI	GCAGC	6 cut(s)	309, 898, 1420, 1432, 2494, 2880
Bsh1236I	CGCG	2 cut(s)	735, 2914
BshFI	GGCC	5 cut(s)	26, 78, 305, 1259, 1625
BshNI	GGYRCC	2 cut(s)	185, 353
BshTI	ACCGGT	2 cut(s)	34, 106
BshVI	ATCGAT	1 cut(s)	521
BsiHKAI	GWGCWC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
BsiSI	CCGG	4 cut(s)	35, 107, 1690, 2795
BslFI	GGGAC	5 cut(s)	182, 650, 1289, 1628, 1832
BslI	CCNNNNNNNGG	6 cut(s)	85, 113, 815, 1183, 1705, 2903
BsmAI	GTCTC	6 cut(s)	399, 1467, 1491, 1757, 2114, 2654
BsmBI	CGTCTC	1 cut(s)	2654
BsmFI	GGGAC	5 cut(s)	182, 650, 1289, 1628, 1832
BsmI	GAATGC	2 cut(s)	1365, 2346
BsnI	GGCC	5 cut(s)	26, 78, 305, 1259, 1625
Bso31I	GGTCTC	1 cut(s)	1757
Bsp1286I	GDGCHC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
Bsp1407I	TGTACA	1 cut(s)	2707
BspACI	CCGC	8 cut(s)	96, 733, 814, 1256, 1273, 1930, 2912, 2914
BspANI	GGCC	5 cut(s)	26, 78, 305, 1259, 1625
BspCNI	CTCAG	5 cut(s)	313, 576, 1209, 1219, 2800
BspDI	ATCGAT	1 cut(s)	521
BspFNI	CGCG	2 cut(s)	735, 2914
BspLI	GGNNCC	6 cut(s)	77, 100, 187, 355, 664, 2076
BspMAI	CTGCAG	3 cut(s)	144, 916, 1438
BspMI	ACCTGC	1 cut(s)	337
BspOI	GCTAGC	1 cut(s)	2270
BspPI	GGATC	4 cut(s)	220, 2069, 2082, 2515
BspT107I	GGYRCC	2 cut(s)	185, 353
BspTI	CTTAAG	1 cut(s)	2560
BspTNI	GGTCTC	1 cut(s)	1757
BsrDI	GCAATG	2 cut(s)	452, 1016
BsrFI	RCCGGY	2 cut(s)	34, 106
BsrGI	TGTACA	1 cut(s)	2707
BsrI	ACTGG	9 cut(s)	413, 538, 727, 911, 1321, 1555, 1595, 1753, 2526
BssAI	RCCGGY	2 cut(s)	34, 106
BssECI	CCNNGG	6 cut(s)	160, 809, 1065, 2087, 2795, 2912
BssNI	GRCGYC	2 cut(s)	390, 2215
BssSI	CACGAG	1 cut(s)	2768
BssT1I	CCWWGG	3 cut(s)	809, 1065, 2087
Bst2BI	CACGAG	1 cut(s)	2768
Bst2UI	CCWGG	3 cut(s)	86, 161, 1642
Bst6I	CTCTTC	2 cut(s)	2112, 2610
BstACI	GRCGYC	2 cut(s)	390, 2215
BstAFI	CTTAAG	1 cut(s)	2560
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstAUI	TGTACA	1 cut(s)	2707
BstBAI	YACGTR	1 cut(s)	1512
BstC8I	GCNNGC	8 cut(s)	28, 281, 1650, 1740, 2241, 2268, 2451, 2866
BstDEI	CTNAG	6 cut(s)	300, 584, 1196, 1227, 1784, 2808
BstDSI	CCRYGG	1 cut(s)	2912
BstFNI	CGCG	2 cut(s)	735, 2914
BstMAI	GTCTC	6 cut(s)	399, 1467, 1491, 1757, 2114, 2654
BstMWI	GCNNNNNNNGC	9 cut(s)	27, 295, 805, 827, 1420, 1442, 2245, 2365, 2888
BstNI	CCWGG	3 cut(s)	86, 161, 1642
BstNSI	RCATGY	3 cut(s)	796, 1742, 2714
BstSCI	CCNGG	4 cut(s)	84, 159, 1640, 2794
BstSFI	CTRYAG	3 cut(s)	140, 912, 1434
BstUI	CGCG	2 cut(s)	735, 2914
BstV1I	GCAGC	6 cut(s)	309, 898, 1420, 1432, 2494, 2880
BstV2I	GAAGAC	3 cut(s)	1953, 2025, 2237
BstX2I	RGATCY	3 cut(s)	2074, 2281, 2507
BstXI	CCANNNNNNTGG	1 cut(s)	2589
BstYI	RGATCY	3 cut(s)	2074, 2281, 2507
Bsu15I	ATCGAT	1 cut(s)	521
Bsu36I	CCTNAGG	1 cut(s)	300
BsuI	GTATCC	1 cut(s)	2396
BsuRI	GGCC	5 cut(s)	26, 78, 305, 1259, 1625
BsuTUI	ATCGAT	1 cut(s)	521
BtgI	CCRYGG	1 cut(s)	2912
BtsI	GCAGTG	2 cut(s)	2149, 2234
BtsIMutI	CAGTG	7 cut(s)	77, 1507, 2149, 2234, 2418, 2530, 2897
BveI	ACCTGC	1 cut(s)	337
Cac8I	GCNNGC	8 cut(s)	28, 281, 1650, 1740, 2241, 2268, 2451, 2866
CaiI	CAGNNNCTG	3 cut(s)	860, 920, 2532
Cfr10I	RCCGGY	2 cut(s)	34, 106
Cfr13I	GGNCC	3 cut(s)	24, 76, 725
Cfr42I	CCGCGG	1 cut(s)	2915
ClaI	ATCGAT	1 cut(s)	521
CseI	GACGC	2 cut(s)	272, 2204
Csp6I	GTAC	7 cut(s)	223, 540, 759, 1040, 2162, 2708, 2909
CspAI	ACCGGT	2 cut(s)	34, 106
CviAII	CATG	9 cut(s)	335, 793, 1537, 1739, 1884, 2033, 2427, 2432, 2711
CviQI	GTAC	7 cut(s)	223, 540, 759, 1040, 2162, 2708, 2909
DdeI	CTNAG	6 cut(s)	300, 584, 1196, 1227, 1784, 2808
DraI	TTTAAA	2 cut(s)	670, 678
DraIII	CACNNNGTG	1 cut(s)	72
EaeI	YGGCCR	1 cut(s)	1623
Eam1104I	CTCTTC	2 cut(s)	2112, 2610
EarI	CTCTTC	2 cut(s)	2112, 2610
Ecl136II	GAGCTC	4 cut(s)	172, 1556, 2038, 2746
Eco130I	CCWWGG	3 cut(s)	809, 1065, 2087
Eco24I	GRGCYC	4 cut(s)	174, 1558, 2040, 2748
Eco31I	GGTCTC	1 cut(s)	1757
Eco32I	GATATC	1 cut(s)	1072
Eco47I	GGWCC	1 cut(s)	725
Eco53kI	GAGCTC	4 cut(s)	172, 1556, 2038, 2746
Eco57I	CTGAAG	3 cut(s)	34, 2003, 2591
Eco81I	CCTNAGG	1 cut(s)	300
EcoICRI	GAGCTC	4 cut(s)	172, 1556, 2038, 2746
EcoRII	CCWGG	3 cut(s)	84, 159, 1640
EcoRV	GATATC	1 cut(s)	1072
EcoT14I	CCWWGG	3 cut(s)	809, 1065, 2087
EcoT22I	ATGCAT	2 cut(s)	1018, 2227
EcoT38I	GRGCYC	4 cut(s)	174, 1558, 2040, 2748
ErhI	CCWWGG	3 cut(s)	809, 1065, 2087
Esp3I	CGTCTC	1 cut(s)	2654
FaeI	CATG	9 cut(s)	338, 796, 1540, 1742, 1887, 2036, 2430, 2435, 2714
FalI	AAGNNNNNCTT	2 cut(s)	2193, 2225
FaqI	GGGAC	5 cut(s)	182, 650, 1289, 1628, 1832
FatI	CATG	9 cut(s)	334, 792, 1536, 1738, 1883, 2032, 2426, 2431, 2710
FauI	CCCGC	2 cut(s)	807, 1266
FauNDI	CATATG	3 cut(s)	176, 1416, 1814
FbaI	TGATCA	2 cut(s)	2146, 2730
Fnu4HI	GCNGC	8 cut(s)	97, 323, 912, 1257, 1421, 1434, 2483, 2869
FriOI	GRGCYC	4 cut(s)	174, 1558, 2040, 2748
Fsp4HI	GCNGC	8 cut(s)	97, 323, 912, 1257, 1421, 1434, 2483, 2869
FspBI	CTAG	5 cut(s)	434, 1340, 1446, 2267, 2361
GluI	GCNGC	8 cut(s)	97, 323, 912, 1257, 1421, 1434, 2483, 2869
GsuI	CTGGAG	4 cut(s)	430, 1545, 1572, 2396
HaeIII	GGCC	5 cut(s)	26, 78, 305, 1259, 1625
HapII	CCGG	4 cut(s)	35, 107, 1690, 2795
HgaI	GACGC	2 cut(s)	272, 2204
Hin1I	GRCGYC	2 cut(s)	390, 2215
Hin1II	CATG	9 cut(s)	338, 796, 1540, 1742, 1887, 2036, 2430, 2435, 2714
HincII	GTYRAC	2 cut(s)	424, 904
HindII	GTYRAC	2 cut(s)	424, 904
HpaII	CCGG	4 cut(s)	35, 107, 1690, 2795
HphI	GGTGA	5 cut(s)	49, 149, 176, 608, 2823
Hpy166II	GTNNAC	5 cut(s)	157, 424, 904, 2342, 2708
Hpy8I	GTNNAC	5 cut(s)	157, 424, 904, 2342, 2708
Hpy99I	CGWCG	1 cut(s)	288
HpyCH4IV	ACGT	5 cut(s)	234, 390, 1511, 2353, 2444
HpyF10VI	GCNNNNNNNGC	9 cut(s)	27, 295, 805, 827, 1420, 1442, 2245, 2365, 2888
HpyF3I	CTNAG	6 cut(s)	300, 584, 1196, 1227, 1784, 2808
HpySE526I	ACGT	5 cut(s)	234, 390, 1511, 2353, 2444
Hsp92I	GRCGYC	2 cut(s)	390, 2215
Hsp92II	CATG	9 cut(s)	338, 796, 1540, 1742, 1887, 2036, 2430, 2435, 2714
Ksp22I	TGATCA	2 cut(s)	2146, 2730
KspI	CCGCGG	1 cut(s)	2915
LmnI	GCTCC	7 cut(s)	104, 662, 1205, 1553, 1561, 2425, 2584
Lsp1109I	GCAGC	6 cut(s)	309, 898, 1420, 1432, 2494, 2880
LweI	GCATC	3 cut(s)	349, 1030, 2307
MaeI	CTAG	5 cut(s)	434, 1340, 1446, 2267, 2361
MaeII	ACGT	5 cut(s)	234, 390, 1511, 2353, 2444
MaeIII	GTNAC	3 cut(s)	227, 2528, 2713
MfeI	CAATTG	2 cut(s)	1155, 1609
MflI	RGATCY	3 cut(s)	2074, 2281, 2507
MhlI	GDGCHC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
MlsI	TGGCCA	1 cut(s)	1625
MluNI	TGGCCA	1 cut(s)	1625
MlyI	GAGTC	4 cut(s)	157, 447, 743, 926
MmeI	TCCRAC	3 cut(s)	853, 1407, 1738
Mox20I	TGGCCA	1 cut(s)	1625
Mph1103I	ATGCAT	2 cut(s)	1018, 2227
MroXI	GAANNNNTTC	2 cut(s)	709, 1220
MscI	TGGCCA	1 cut(s)	1625
MslI	CAYNNNNRTG	4 cut(s)	162, 333, 1374, 2587
Msp20I	TGGCCA	1 cut(s)	1625
MspA1I	CMGCKG	2 cut(s)	1423, 2914
MspCI	CTTAAG	1 cut(s)	2560
MspI	CCGG	4 cut(s)	35, 107, 1690, 2795
MspR9I	CCNGG	4 cut(s)	86, 161, 1642, 2796
MunI	CAATTG	2 cut(s)	1155, 1609
Mva1269I	GAATGC	2 cut(s)	1365, 2346
MvaI	CCWGG	3 cut(s)	86, 161, 1642
MvnI	CGCG	2 cut(s)	735, 2914
MwoI	GCNNNNNNNGC	9 cut(s)	27, 295, 805, 827, 1420, 1442, 2245, 2365, 2888
NciI	CCSGG	1 cut(s)	2796
NdeI	CATATG	3 cut(s)	176, 1416, 1814
NheI	GCTAGC	1 cut(s)	2266
NlaIII	CATG	9 cut(s)	338, 796, 1540, 1742, 1887, 2036, 2430, 2435, 2714
NlaIV	GGNNCC	6 cut(s)	77, 100, 187, 355, 664, 2076
NmuCI	GTSAC	2 cut(s)	2528, 2713
NsiI	ATGCAT	2 cut(s)	1018, 2227
NspI	RCATGY	3 cut(s)	796, 1742, 2714
OliI	CACNNNNGTG	2 cut(s)	162, 1374
PaeI	GCATGC	1 cut(s)	1742
PciI	ACATGT	1 cut(s)	2710
PcsI	WCGNNNNNNNCGW	2 cut(s)	1834, 2350
PctI	GAATGC	2 cut(s)	1365, 2346
PdmI	GAANNNNTTC	2 cut(s)	709, 1220
PfeI	GAWTC	9 cut(s)	467, 599, 983, 1084, 1192, 1231, 1880, 2821, 2828
PflFI	GACNNNGTC	1 cut(s)	620
PflMI	CCANNNNNTGG	2 cut(s)	85, 1705
PinAI	ACCGGT	2 cut(s)	34, 106
PkrI	GCNGC	8 cut(s)	98, 324, 913, 1258, 1422, 1435, 2484, 2870
PleI	GAGTC	4 cut(s)	156, 446, 743, 925
PpsI	GAGTC	4 cut(s)	156, 446, 743, 925
Ppu21I	YACGTR	1 cut(s)	1512
PscI	ACATGT	1 cut(s)	2710
PshBI	ATTAAT	2 cut(s)	951, 2333
Psp124BI	GAGCTC	4 cut(s)	174, 1558, 2040, 2748
Psp1406I	AACGTT	1 cut(s)	2444
Psp6I	CCWGG	3 cut(s)	84, 159, 1640
PspGI	CCWGG	3 cut(s)	84, 159, 1640
PspN4I	GGNNCC	6 cut(s)	77, 100, 187, 355, 664, 2076
PspPI	GGNCC	3 cut(s)	24, 76, 725
PstI	CTGCAG	3 cut(s)	144, 916, 1438
PstNI	CAGNNNCTG	3 cut(s)	860, 920, 2532
PsuI	RGATCY	3 cut(s)	2074, 2281, 2507
PsyI	GACNNNGTC	1 cut(s)	620
PvuII	CAGCTG	1 cut(s)	1423
RsaI	GTAC	7 cut(s)	224, 541, 760, 1041, 2163, 2709, 2910
RsaNI	GTAC	7 cut(s)	223, 540, 759, 1040, 2162, 2708, 2909
RseI	CAYNNNNRTG	4 cut(s)	162, 333, 1374, 2587
SacI	GAGCTC	4 cut(s)	174, 1558, 2040, 2748
SacII	CCGCGG	1 cut(s)	2915
SatI	GCNGC	8 cut(s)	97, 323, 912, 1257, 1421, 1434, 2483, 2869
Sau96I	GGNCC	3 cut(s)	24, 76, 725
ScaI	AGTACT	1 cut(s)	760
SchI	GAGTC	4 cut(s)	157, 447, 743, 926
ScrFI	CCNGG	4 cut(s)	86, 161, 1642, 2796
SduI	GDGCHC	6 cut(s)	174, 1558, 1654, 2040, 2422, 2748
SfaNI	GCATC	3 cut(s)	349, 1030, 2307
SfcI	CTRYAG	3 cut(s)	140, 912, 1434
Sfr303I	CCGCGG	1 cut(s)	2915
SgrBI	CCGCGG	1 cut(s)	2915
SinI	GGWCC	1 cut(s)	725
SmiMI	CAYNNNNRTG	4 cut(s)	162, 333, 1374, 2587
SmlI	CTYRAG	3 cut(s)	1033, 2560, 2883
SmoI	CTYRAG	3 cut(s)	1033, 2560, 2883
SphI	GCATGC	1 cut(s)	1742
SsiI	CCGC	8 cut(s)	96, 733, 814, 1256, 1273, 1930, 2912, 2914
SspI	AATATT	1 cut(s)	1170
SspMI	CTAG	5 cut(s)	434, 1340, 1446, 2267, 2361
SstI	GAGCTC	4 cut(s)	174, 1558, 2040, 2748
StyD4I	CCNGG	4 cut(s)	84, 159, 1640, 2794
StyI	CCWWGG	3 cut(s)	809, 1065, 2087
TaiI	ACGT	5 cut(s)	237, 393, 1514, 2356, 2447
TaqI	TCGA	7 cut(s)	250, 521, 747, 1654, 1901, 2115, 2197
TatI	WGTACW	5 cut(s)	222, 758, 1039, 2161, 2707
TauI	GCSGC	2 cut(s)	99, 1259
TfiI	GAWTC	9 cut(s)	467, 599, 983, 1084, 1192, 1231, 1880, 2821, 2828
TscAI	CASTG	7 cut(s)	77, 1507, 2156, 2241, 2418, 2537, 2904
TseFI	GTSAC	2 cut(s)	2528, 2713
TseI	GCWGC	6 cut(s)	322, 911, 1420, 1433, 2482, 2868
Tsp45I	GTSAC	2 cut(s)	2528, 2713
TspGWI	ACGGA	4 cut(s)	357, 770, 952, 2919
TspRI	CASTG	7 cut(s)	77, 1507, 2156, 2241, 2418, 2537, 2904
Tth111I	GACNNNGTC	1 cut(s)	620
Van91I	CCANNNNNTGG	2 cut(s)	85, 1705
Vha464I	CTTAAG	1 cut(s)	2560
VpaK11BI	GGWCC	1 cut(s)	725
VspI	ATTAAT	2 cut(s)	951, 2333
XapI	RAATTY	7 cut(s)	448, 489, 1217, 1380, 1763, 1857, 2551
XceI	RCATGY	3 cut(s)	796, 1742, 2714
XmnI	GAANNNNTTC	2 cut(s)	709, 1220
XspI	CTAG	5 cut(s)	434, 1340, 1446, 2267, 2361
ZraI	GACGTC	1 cut(s)	391
ZrmI	AGTACT	1 cut(s)	760
Zsp2I	ATGCAT	2 cut(s)	1018, 2227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.