Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0009188

Physical Location & Seq

Genomic Coordinates Reverse (-)

7019710 .. 7034158

Gene Structure 14449 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00390950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGGTAGAATTGGGAATGAGCAAGGCCAGCAAACCGGTGAGTGTGAGCTTCAGGATACCATATTACACACAGTGGGGCCAAAGCCTGGTGGTGTGCGGCTCCGAACCGGTGCTTGGTTCATGGAATGTGAAGAGGGGTGTGCTGTTGAGTCCTGTTCACCAGGGTGATGAGCTCATATGGTTTGGCACCATTTCTGTCCCTAAAGGGTTTGGATCGTGCGAGTACAGTTACTACGTTGTGGATGATGATCGAAATGTTCTTCGATGGGAGATGGGAAACAAGCGTCGGCTTCTTTTGCCTCTCGCCATTCAGGAAGGAGAGCTGCTTCACTTCCATGACTTTTGGCAGGTTGGTGCCGATGCAATTCCTTTCAGAAGTGCCTTCAAGGACGTCATCTTTCGTAGAGAGCGGAGTTTGGAGGTTGAGAAACCTCTAGGAGTCATCCAAAATTCACTGCAGAATGATGATTCTGTTCTCGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGGAACATCGATTTACATTACTGGTAGTACCTCAAAGTTGGGGAATTGGAAGGCACAGGATGGGCTTAAACTGAGCTATTCTGGTGAATCTACTTGGCATGGAGACTGTGTCTTGCTGAAAGGGGACTTCCCGATAAAATATAAGTACTGTAAATATAGCAAAGGAGGAAACATTTCTTCTGAAACTGGTCCAAACCGCGAAATTGCTCTCGACTCGTTAAGTACTGAACCAAGATACCTTTTCCGTTCAGATGGCATGTTGCGAGAAATGCCTTGGCGGGGGGCTGGTGTTGCAATCCCAATGTTCTCTGTTAGATCAGAAACTGATCTTGGTGTTGGAGAGTTTCTTGACCTGAAATTACTTGTTGACTGGGCTGCAGAGTCTGGCTTTCATTTAGTTCAGCTTTTACCCATTAATGATACATCCGTGTATGGAATGTGGTGGGATTCATATCCTTACAGCTCACTTTCTGTATATGCATTGCATCCTTTATACTTGAGAGTACAGGCACTTTCAGAAAACATCCCCAAGGATATCAAGGTAGAGATTCAGAAGGCAAAAGAAGAATTGGAGGGAAAGGATGTTGATTACGAGGCTACCTTGACCACTAAACTCTCAATTGCAAAGAAAATATTTGCCCAAGAGAAGGATAAGATTCTCAGCTCCAATTCGTTCCAGAAATTTTTCTCTGAGAATCAGGAATGGTTAAAACCCTATGCGGCCTTCTGTTTTTTGCGGGACTTCTTTGAAACATCAGATCACAGTCAATGGGGTCGGTTTTCCCAGTTTTCAATAGAAAAGCTAGAGAAGCTCATCTCTAAAGACAGCATTCACTATTGTGAAATTTGCTTCCATTATTACATCCAATTTCATTTGCATATGCAGCTGTCGGAAGCTGCAGAATATGCAAGAAAGAAAGGAGTAGTATTGAAAGGAGACTTGCCTATTGGAGTCGGTAGAGACAGTGTAGATACATGGGTCTATCCGAATCTGTTTCGCCTGAACACATCTACTGGAGCTCCTCCAGATTATTTTGATAAAAATGGGCAGAACTGGGGTTTCCCTACTTACAATTGGGACGAGATGGCCAAAGACAATTATGCTTGGTGGCGTGCTCGATTGACACAGATGGCAAAATACTTTACAGCTTACCGAATTGACCATATATTGGGGTTCTTTCGTATCTGGGAACTCCCAGAGCATGCCATTACTGGTCTCGTTGGAAAATTTCGTCCTTCCATTCCTTTAAGTCAGGAAGAACTTGAAAGAGAGGGCATATGGGATTTTGATCGCTTGGCTCGTCCATATATCCCACAGGAAATTTTACAGATTAAGTTTGGAGATTCATGGAGTTTTATTGCTTCGAGTTTTCTGAATGAATATCAGAAGAACCGCTATGAGTTTAAGGAAGACTGCAACACAGAGAAAAAAATTGCTTCCAAGCTGAAGTCATTTTCAGGAAGATCATTGTTGCAGAATGAAGACCACATTAGGCATGAGCTCTTTGATATTTTACAGAATATAGTTCTGATAAGAGATCCAGAAAATCCAAGGAACTTCTATCCTCGCTTCAATCTCGAAGAGACTTCAAGTTTTAAGGATTTGGATGATCACTGCAAGAATGTACTGAAACGGTTATACTATGATTACTACTTCCATCGACAAGAAAATCTTTGGCGTCAAAATGCATTGAAGACACTGCCTGCTCTGCTGAACTCATCGGATATGCTAGCTTGTGGGGAAGATCTGGGGCTTATTCCATCTTGTGTTTATCCTGTGATGCAAGAACTGGGATTAATAGGTTTACGCATTCAACGTATGCCTAGTGAGCCTGATCTGGAGTTTGGTATCCCTTCTCGATACAATTATATGACAGTGTGTGCTCCATCATGCCATGACTGTTCAACGTTGCGTGCTTGGTGGGAAGAAGATGAAGAAAGGAGGCAGCGATTTTTTAGCAGTATGGTGGGATCTGACTTGTTGCCACCCAGTCGCTGTGTTCCAGAGATTGCAAATTTCATCTTAAGGCAACATTTTGAAGCTCCATCAATGTGGGCAATTTTTCCTCTTCAGGATTTGTTGATTTTGAAAGAAGAATACACAACACGCCCTGCAAGAGAGGAGACAATCAATGACCCGACAAATCCGAAGCATTATTGGAGATACCGTGTACATGTGACATTGGAGGCTTTGATCAAGGATAAAGAGCTCACATCGATCATCAAAGACCTCGTGCGTGGAAGTGGAAGATCAAATCCGGGGAAACAAGCTGAGAAACAAGTGATTCAGGAATCACCTGTTGCAACCACAGAGAAGAAGCAGATTGCCAGCAGCAAAGAAAAGGCTCTAGTGGCAACTCCACTGAACGGAGTGCCGCGGAAGGAGGCTGTTGCTGTTTCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0010297 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0030246 GO:0030247 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

CBM_20 Glyco_hydro_77

Protein Analysis

972

Amino Acids

112.3

Weight (kDa)

6.07

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	13 - 101	3.6e-21	Starch binding domain
CBM_20	PF00686	158 - 242	2.1e-15	Starch binding domain
Glyco_hydro_77	PF02446	270 - 897	5.9e-145	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40840
fragaria_vesca	FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260
malus_domestica	MD00G1025200.v1.1
prunus_persica	Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1
pyrus_communis	pycom12g09050
rosa_chinensis	RchiOBHm_Chr3g0494731
rosa_laevigata	RLG00000022663
rosa_multiflora	Rmu_sc0000198.1_g000016
rosa_roxburghii	Rroxscaffold_6G00390950
rosa_rugosa	Rorug03G0268100
rosa_samantha	Rh3AG316600 Rh3BG351900 Rh3CG349000 Rh3DG351500
rosa_wichuraiana	Rw3G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	393
Acc36I	ACCTGC	1 cut(s)	337
AccB1I	GGYRCC	2 cut(s)	185, 353
AccB7I	CCANNNNNTGG	2 cut(s)	85, 1681
AccBSI	CCGCTC	1 cut(s)	409
AccII	CGCG	2 cut(s)	711, 2890
AciI	CCGC	9 cut(s)	96, 409, 709, 790, 1232, 1249, 1906, 2888, 2890
AclI	AACGTT	1 cut(s)	2420
AclWI	GGATC	3 cut(s)	220, 2045, 2491
AcoI	YGGCCR	1 cut(s)	1599
AcsI	RAATTY	7 cut(s)	448, 489, 1193, 1356, 1739, 1833, 2527
AcuI	CTGAAG	3 cut(s)	34, 1979, 2567
AcyI	GRCGYC	2 cut(s)	390, 2191
AdeI	CACNNNGTG	1 cut(s)	72
AfaI	GTAC	7 cut(s)	224, 541, 659, 736, 1017, 2139, 2685
AfiI	CCNNNNNNNGG	6 cut(s)	85, 113, 791, 1159, 1681, 2879
AflII	CTTAAG	1 cut(s)	2536
AflIII	ACRYGT	1 cut(s)	2686
AgeI	ACCGGT	2 cut(s)	34, 106
AjnI	CCWGG	2 cut(s)	84, 159
AjuI	GAANNNNNNNTTGG	4 cut(s)	96, 128, 825, 857
AleI	CACNNNNGTG	2 cut(s)	162, 1350
Alw21I	GWGCWC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
Alw26I	GTCTC	6 cut(s)	609, 1443, 1467, 1733, 2090, 2630
AlwI	GGATC	3 cut(s)	220, 2045, 2491
AlwNI	CAGNNNCTG	3 cut(s)	836, 896, 2508
AoxI	GGCC	4 cut(s)	24, 76, 1233, 1599
ApeKI	GCWGC	6 cut(s)	322, 887, 1396, 1409, 2458, 2844
ApoI	RAATTY	7 cut(s)	448, 489, 1193, 1356, 1739, 1833, 2527
AseI	ATTAAT	2 cut(s)	927, 2309
AsiGI	ACCGGT	2 cut(s)	34, 106
Asp700I	GAANNNNTTC	2 cut(s)	685, 1196
AspS9I	GGNCC	2 cut(s)	76, 701
AsuC2I	CCSGG	1 cut(s)	2772
AsuHPI	GGTGA	5 cut(s)	49, 149, 176, 608, 2799
AsuNHI	GCTAGC	1 cut(s)	2242
AvaII	GGWCC	1 cut(s)	701
BaeI	ACNNNNGTAYC	4 cut(s)	924, 957, 1476, 1509
BalI	TGGCCA	1 cut(s)	1601
BanI	GGYRCC	2 cut(s)	185, 353
BanII	GRGCYC	4 cut(s)	174, 1534, 2016, 2724
BauI	CACGAG	1 cut(s)	2744
BbsI	GAAGAC	3 cut(s)	1929, 2001, 2213
Bbv12I	GWGCWC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
BbvI	GCAGC	6 cut(s)	309, 874, 1396, 1408, 2470, 2856
BciT130I	CCWGG	2 cut(s)	86, 161
BciVI	GTATCC	2 cut(s)	48, 2372
BclI	TGATCA	2 cut(s)	2122, 2706
BcnI	CCSGG	1 cut(s)	2772
BcoDI	GTCTC	6 cut(s)	609, 1443, 1467, 1733, 2090, 2630
BfaI	CTAG	5 cut(s)	434, 1316, 2243, 2337, 2861
BfmI	CTRYAG	3 cut(s)	455, 888, 1410
BfrI	CTTAAG	1 cut(s)	2536
BfuAI	ACCTGC	1 cut(s)	337
BfuI	GTATCC	2 cut(s)	48, 2372
BglII	AGATCT	1 cut(s)	2257
BisI	GCNGC	9 cut(s)	97, 323, 888, 1233, 1397, 1410, 2459, 2845, 2888
BlsI	GCNGC	9 cut(s)	98, 324, 889, 1234, 1398, 1411, 2460, 2846, 2889
BmcAI	AGTACT	2 cut(s)	659, 736
Bme1390I	CCNGG	3 cut(s)	86, 161, 2772
Bme18I	GGWCC	1 cut(s)	701
BmgT120I	GGNCC	2 cut(s)	76, 701
BmiI	GGNNCC	4 cut(s)	77, 100, 187, 355
BmrFI	CCNGG	3 cut(s)	86, 161, 2772
BmrI	ACTGGG	5 cut(s)	892, 1291, 1576, 2312, 2496
BmsI	GCATC	3 cut(s)	349, 1006, 2283
BmtI	GCTAGC	1 cut(s)	2246
BmuI	ACTGGG	5 cut(s)	892, 1291, 1576, 2312, 2496
BpiI	GAAGAC	3 cut(s)	1929, 2001, 2213
BpmI	CTGGAG	3 cut(s)	1521, 1548, 2372
BpuEI	CTTGAG	1 cut(s)	1030
BpuMI	CCSGG	1 cut(s)	2772
Bsa29I	ATCGAT	2 cut(s)	521, 2729
BsaBI	GATNNNNATC	3 cut(s)	246, 963, 1800
BsaHI	GRCGYC	2 cut(s)	390, 2191
BsaI	GGTCTC	1 cut(s)	1733
BsaJI	CCNNGG	6 cut(s)	160, 785, 1041, 2063, 2771, 2888
BsaWI	WCCGGW	2 cut(s)	34, 106
BsaXI	ACNNNNNCTCC	6 cut(s)	606, 636, 1516, 1546, 2665, 2695
Bsc4I	CCNNNNNNNGG	6 cut(s)	85, 113, 791, 1159, 1681, 2879
Bse118I	RCCGGY	2 cut(s)	34, 106
Bse1I	ACTGG	9 cut(s)	538, 703, 887, 1297, 1531, 1571, 1729, 2307, 2502
Bse3DI	GCAATG	1 cut(s)	992
Bse8I	GATNNNNATC	3 cut(s)	246, 963, 1800
BseBI	CCWGG	2 cut(s)	86, 161
BseCI	ATCGAT	2 cut(s)	521, 2729
BseDI	CCNNGG	6 cut(s)	160, 785, 1041, 2063, 2771, 2888
BseGI	GGATG	9 cut(s)	247, 441, 577, 935, 997, 1035, 1099, 1374, 2125
BseJI	GATNNNNATC	3 cut(s)	246, 963, 1800
BseLI	CCNNNNNNNGG	6 cut(s)	85, 113, 791, 1159, 1681, 2879
BseMI	GCAATG	1 cut(s)	992
BseMII	CTCAG	4 cut(s)	575, 1186, 1194, 2775
BseNI	ACTGG	9 cut(s)	538, 703, 887, 1297, 1531, 1571, 1729, 2307, 2502
BseRI	GAGGAG	2 cut(s)	1524, 2648
BseXI	GCAGC	6 cut(s)	309, 874, 1396, 1408, 2470, 2856
Bsh1236I	CGCG	2 cut(s)	711, 2890
BshFI	GGCC	4 cut(s)	26, 78, 1235, 1601
BshNI	GGYRCC	2 cut(s)	185, 353
BshTI	ACCGGT	2 cut(s)	34, 106
BshVI	ATCGAT	2 cut(s)	521, 2729
BsiHKAI	GWGCWC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
BsiSI	CCGG	3 cut(s)	35, 107, 2771
BslFI	GGGAC	4 cut(s)	182, 650, 1265, 1604
BslI	CCNNNNNNNGG	6 cut(s)	85, 113, 791, 1159, 1681, 2879
BsmAI	GTCTC	6 cut(s)	609, 1443, 1467, 1733, 2090, 2630
BsmFI	GGGAC	4 cut(s)	182, 650, 1265, 1604
BsmI	GAATGC	2 cut(s)	1341, 2322
BsnI	GGCC	4 cut(s)	26, 78, 1235, 1601
Bso31I	GGTCTC	1 cut(s)	1733
Bsp1286I	GDGCHC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
Bsp1407I	TGTACA	1 cut(s)	2683
BspACI	CCGC	9 cut(s)	96, 409, 709, 790, 1232, 1249, 1906, 2888, 2890
BspANI	GGCC	4 cut(s)	26, 78, 1235, 1601
BspCNI	CTCAG	4 cut(s)	576, 1185, 1195, 2776
BspDI	ATCGAT	2 cut(s)	521, 2729
BspFNI	CGCG	2 cut(s)	711, 2890
BspLI	GGNNCC	4 cut(s)	77, 100, 187, 355
BspMAI	CTGCAG	3 cut(s)	459, 892, 1414
BspMI	ACCTGC	1 cut(s)	337
BspOI	GCTAGC	1 cut(s)	2246
BspPI	GGATC	3 cut(s)	220, 2045, 2491
BspT107I	GGYRCC	2 cut(s)	185, 353
BspTI	CTTAAG	1 cut(s)	2536
BspTNI	GGTCTC	1 cut(s)	1733
BsrBI	CCGCTC	1 cut(s)	409
BsrDI	GCAATG	1 cut(s)	992
BsrFI	RCCGGY	2 cut(s)	34, 106
BsrGI	TGTACA	1 cut(s)	2683
BsrI	ACTGG	9 cut(s)	538, 703, 887, 1297, 1531, 1571, 1729, 2307, 2502
BssAI	RCCGGY	2 cut(s)	34, 106
BssECI	CCNNGG	6 cut(s)	160, 785, 1041, 2063, 2771, 2888
BssNI	GRCGYC	2 cut(s)	390, 2191
BssSI	CACGAG	1 cut(s)	2744
BssT1I	CCWWGG	3 cut(s)	785, 1041, 2063
Bst2BI	CACGAG	1 cut(s)	2744
Bst2UI	CCWGG	2 cut(s)	86, 161
Bst6I	CTCTTC	3 cut(s)	125, 2088, 2586
BstACI	GRCGYC	2 cut(s)	390, 2191
BstAFI	CTTAAG	1 cut(s)	2536
BstAPI	GCANNNNNTGC	1 cut(s)	1418
BstAUI	TGTACA	1 cut(s)	2683
BstC8I	GCNNGC	7 cut(s)	28, 1626, 1716, 2217, 2244, 2427, 2842
BstDEI	CTNAG	4 cut(s)	584, 1172, 1203, 2784
BstDSI	CCRYGG	1 cut(s)	2888
BstF5I	GGATG	9 cut(s)	247, 441, 577, 935, 997, 1035, 1099, 1374, 2125
BstFNI	CGCG	2 cut(s)	711, 2890
BstMAI	GTCTC	6 cut(s)	609, 1443, 1467, 1733, 2090, 2630
BstNI	CCWGG	2 cut(s)	86, 161
BstNSI	RCATGY	3 cut(s)	772, 1718, 2690
BstSCI	CCNGG	3 cut(s)	84, 159, 2770
BstSFI	CTRYAG	3 cut(s)	455, 888, 1410
BstUI	CGCG	2 cut(s)	711, 2890
BstV1I	GCAGC	6 cut(s)	309, 874, 1396, 1408, 2470, 2856
BstV2I	GAAGAC	3 cut(s)	1929, 2001, 2213
BstX2I	RGATCY	3 cut(s)	2050, 2257, 2483
BstXI	CCANNNNNNTGG	1 cut(s)	2565
BstYI	RGATCY	3 cut(s)	2050, 2257, 2483
Bsu15I	ATCGAT	2 cut(s)	521, 2729
BsuI	GTATCC	2 cut(s)	48, 2372
BsuRI	GGCC	4 cut(s)	26, 78, 1235, 1601
BsuTUI	ATCGAT	2 cut(s)	521, 2729
BtgI	CCRYGG	1 cut(s)	2888
BtsCI	GGATG	9 cut(s)	247, 441, 577, 935, 997, 1035, 1099, 1374, 2125
BtsI	GCAGTG	3 cut(s)	452, 2125, 2210
BtsIMutI	CAGTG	7 cut(s)	77, 452, 1483, 2125, 2210, 2394, 2873
BveI	ACCTGC	1 cut(s)	337
Cac8I	GCNNGC	7 cut(s)	28, 1626, 1716, 2217, 2244, 2427, 2842
CaiI	CAGNNNCTG	3 cut(s)	836, 896, 2508
Cfr10I	RCCGGY	2 cut(s)	34, 106
Cfr13I	GGNCC	2 cut(s)	76, 701
Cfr42I	CCGCGG	1 cut(s)	2891
ClaI	ATCGAT	2 cut(s)	521, 2729
CseI	GACGC	2 cut(s)	272, 2180
Csp6I	GTAC	7 cut(s)	223, 540, 658, 735, 1016, 2138, 2684
CspAI	ACCGGT	2 cut(s)	34, 106
CviQI	GTAC	7 cut(s)	223, 540, 658, 735, 1016, 2138, 2684
DdeI	CTNAG	4 cut(s)	584, 1172, 1203, 2784
DraIII	CACNNNGTG	1 cut(s)	72
EaeI	YGGCCR	1 cut(s)	1599
Eam1104I	CTCTTC	3 cut(s)	125, 2088, 2586
EarI	CTCTTC	3 cut(s)	125, 2088, 2586
Ecl136II	GAGCTC	4 cut(s)	172, 1532, 2014, 2722
Eco130I	CCWWGG	3 cut(s)	785, 1041, 2063
Eco24I	GRGCYC	4 cut(s)	174, 1534, 2016, 2724
Eco31I	GGTCTC	1 cut(s)	1733
Eco32I	GATATC	1 cut(s)	1048
Eco47I	GGWCC	1 cut(s)	701
Eco53kI	GAGCTC	4 cut(s)	172, 1532, 2014, 2722
Eco57I	CTGAAG	3 cut(s)	34, 1979, 2567
EcoICRI	GAGCTC	4 cut(s)	172, 1532, 2014, 2722
EcoRII	CCWGG	2 cut(s)	84, 159
EcoRV	GATATC	1 cut(s)	1048
EcoT14I	CCWWGG	3 cut(s)	785, 1041, 2063
EcoT22I	ATGCAT	2 cut(s)	994, 2203
EcoT38I	GRGCYC	4 cut(s)	174, 1534, 2016, 2724
ErhI	CCWWGG	3 cut(s)	785, 1041, 2063
FalI	AAGNNNNNCTT	2 cut(s)	2169, 2201
FaqI	GGGAC	4 cut(s)	182, 650, 1265, 1604
FauI	CCCGC	2 cut(s)	783, 1242
FauNDI	CATATG	3 cut(s)	176, 1392, 1790
FbaI	TGATCA	2 cut(s)	2122, 2706
Fnu4HI	GCNGC	9 cut(s)	97, 323, 888, 1233, 1397, 1410, 2459, 2845, 2888
FokI	GGATG	9 cut(s)	254, 428, 584, 922, 984, 1022, 1106, 1361, 2132
FriOI	GRGCYC	4 cut(s)	174, 1534, 2016, 2724
Fsp4HI	GCNGC	9 cut(s)	97, 323, 888, 1233, 1397, 1410, 2459, 2845, 2888
FspBI	CTAG	5 cut(s)	434, 1316, 2243, 2337, 2861
GluI	GCNGC	9 cut(s)	97, 323, 888, 1233, 1397, 1410, 2459, 2845, 2888
GsuI	CTGGAG	3 cut(s)	1521, 1548, 2372
HaeIII	GGCC	4 cut(s)	26, 78, 1235, 1601
HapII	CCGG	3 cut(s)	35, 107, 2771
HgaI	GACGC	2 cut(s)	272, 2180
Hin1I	GRCGYC	2 cut(s)	390, 2191
HincII	GTYRAC	1 cut(s)	880
HindII	GTYRAC	1 cut(s)	880
HpaII	CCGG	3 cut(s)	35, 107, 2771
HphI	GGTGA	5 cut(s)	49, 149, 176, 608, 2799
Hpy166II	GTNNAC	4 cut(s)	157, 880, 2318, 2684
Hpy8I	GTNNAC	4 cut(s)	157, 880, 2318, 2684
Hpy99I	CGWCG	1 cut(s)	288
HpyCH4IV	ACGT	4 cut(s)	234, 390, 2329, 2420
HpyF3I	CTNAG	4 cut(s)	584, 1172, 1203, 2784
HpySE526I	ACGT	4 cut(s)	234, 390, 2329, 2420
Hsp92I	GRCGYC	2 cut(s)	390, 2191
Ksp22I	TGATCA	2 cut(s)	2122, 2706
KspI	CCGCGG	1 cut(s)	2891
LmnI	GCTCC	6 cut(s)	104, 1181, 1529, 1537, 2401, 2560
Lsp1109I	GCAGC	6 cut(s)	309, 874, 1396, 1408, 2470, 2856
LweI	GCATC	3 cut(s)	349, 1006, 2283
MaeI	CTAG	5 cut(s)	434, 1316, 2243, 2337, 2861
MaeII	ACGT	4 cut(s)	234, 390, 2329, 2420
MaeIII	GTNAC	2 cut(s)	227, 2689
MbiI	CCGCTC	1 cut(s)	409
MfeI	CAATTG	2 cut(s)	1131, 1585
MflI	RGATCY	3 cut(s)	2050, 2257, 2483
MhlI	GDGCHC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
MlsI	TGGCCA	1 cut(s)	1601
MluNI	TGGCCA	1 cut(s)	1601
MlyI	GAGTC	5 cut(s)	157, 447, 719, 902, 1473
MmeI	TCCRAC	3 cut(s)	829, 1383, 1714
Mox20I	TGGCCA	1 cut(s)	1601
Mph1103I	ATGCAT	2 cut(s)	994, 2203
MroXI	GAANNNNTTC	2 cut(s)	685, 1196
MscI	TGGCCA	1 cut(s)	1601
MslI	CAYNNNNRTG	4 cut(s)	162, 333, 1350, 2563
Msp20I	TGGCCA	1 cut(s)	1601
MspA1I	CMGCKG	2 cut(s)	1399, 2890
MspCI	CTTAAG	1 cut(s)	2536
MspI	CCGG	3 cut(s)	35, 107, 2771
MspR9I	CCNGG	3 cut(s)	86, 161, 2772
MunI	CAATTG	2 cut(s)	1131, 1585
Mva1269I	GAATGC	2 cut(s)	1341, 2322
MvaI	CCWGG	2 cut(s)	86, 161
MvnI	CGCG	2 cut(s)	711, 2890
NciI	CCSGG	1 cut(s)	2772
NdeI	CATATG	3 cut(s)	176, 1392, 1790
NheI	GCTAGC	1 cut(s)	2242
NlaIV	GGNNCC	4 cut(s)	77, 100, 187, 355
NmuCI	GTSAC	1 cut(s)	2689
NsiI	ATGCAT	2 cut(s)	994, 2203
NspI	RCATGY	3 cut(s)	772, 1718, 2690
OliI	CACNNNNGTG	2 cut(s)	162, 1350
PaeI	GCATGC	1 cut(s)	1718
PciI	ACATGT	1 cut(s)	2686
PcsI	WCGNNNNNNNCGW	2 cut(s)	1810, 2326
PctI	GAATGC	2 cut(s)	1341, 2322
PdmI	GAANNNNTTC	2 cut(s)	685, 1196
PflFI	GACNNNGTC	1 cut(s)	620
PflMI	CCANNNNNTGG	2 cut(s)	85, 1681
PinAI	ACCGGT	2 cut(s)	34, 106
PkrI	GCNGC	9 cut(s)	98, 324, 889, 1234, 1398, 1411, 2460, 2846, 2889
PleI	GAGTC	5 cut(s)	156, 446, 719, 901, 1472
PpsI	GAGTC	5 cut(s)	156, 446, 719, 901, 1472
PscI	ACATGT	1 cut(s)	2686
PshBI	ATTAAT	2 cut(s)	927, 2309
Psp124BI	GAGCTC	4 cut(s)	174, 1534, 2016, 2724
Psp1406I	AACGTT	1 cut(s)	2420
Psp6I	CCWGG	2 cut(s)	84, 159
PspGI	CCWGG	2 cut(s)	84, 159
PspN4I	GGNNCC	4 cut(s)	77, 100, 187, 355
PspPI	GGNCC	2 cut(s)	76, 701
PstI	CTGCAG	3 cut(s)	459, 892, 1414
PstNI	CAGNNNCTG	3 cut(s)	836, 896, 2508
PsuI	RGATCY	3 cut(s)	2050, 2257, 2483
PsyI	GACNNNGTC	1 cut(s)	620
PvuII	CAGCTG	1 cut(s)	1399
RsaI	GTAC	7 cut(s)	224, 541, 659, 736, 1017, 2139, 2685
RsaNI	GTAC	7 cut(s)	223, 540, 658, 735, 1016, 2138, 2684
RseI	CAYNNNNRTG	4 cut(s)	162, 333, 1350, 2563
SacI	GAGCTC	4 cut(s)	174, 1534, 2016, 2724
SacII	CCGCGG	1 cut(s)	2891
SatI	GCNGC	9 cut(s)	97, 323, 888, 1233, 1397, 1410, 2459, 2845, 2888
Sau96I	GGNCC	2 cut(s)	76, 701
ScaI	AGTACT	2 cut(s)	659, 736
SchI	GAGTC	5 cut(s)	157, 447, 719, 902, 1473
ScrFI	CCNGG	3 cut(s)	86, 161, 2772
SduI	GDGCHC	6 cut(s)	174, 1534, 1630, 2016, 2398, 2724
SfaNI	GCATC	3 cut(s)	349, 1006, 2283
SfcI	CTRYAG	3 cut(s)	455, 888, 1410
Sfr303I	CCGCGG	1 cut(s)	2891
SgrBI	CCGCGG	1 cut(s)	2891
SinI	GGWCC	1 cut(s)	701
SmiMI	CAYNNNNRTG	4 cut(s)	162, 333, 1350, 2563
SmlI	CTYRAG	2 cut(s)	1009, 2536
SmoI	CTYRAG	2 cut(s)	1009, 2536
SphI	GCATGC	1 cut(s)	1718
SsiI	CCGC	9 cut(s)	96, 409, 709, 790, 1232, 1249, 1906, 2888, 2890
SspI	AATATT	1 cut(s)	1146
SspMI	CTAG	5 cut(s)	434, 1316, 2243, 2337, 2861
SstI	GAGCTC	4 cut(s)	174, 1534, 2016, 2724
StyD4I	CCNGG	3 cut(s)	84, 159, 2770
StyI	CCWWGG	3 cut(s)	785, 1041, 2063
TaiI	ACGT	4 cut(s)	237, 393, 2332, 2423
TatI	WGTACW	6 cut(s)	222, 657, 734, 1015, 2137, 2683
TauI	GCSGC	3 cut(s)	99, 1235, 2890
TscAI	CASTG	7 cut(s)	77, 459, 1483, 2132, 2217, 2394, 2880
TseFI	GTSAC	1 cut(s)	2689
TseI	GCWGC	6 cut(s)	322, 887, 1396, 1409, 2458, 2844
Tsp45I	GTSAC	1 cut(s)	2689
TspDTI	ATGAA	9 cut(s)	108, 893, 951, 1373, 1848, 1905, 2007, 2460, 2521
TspGWI	ACGGA	3 cut(s)	746, 928, 2895
TspRI	CASTG	7 cut(s)	77, 459, 1483, 2132, 2217, 2394, 2880
Tth111I	GACNNNGTC	1 cut(s)	620
Van91I	CCANNNNNTGG	2 cut(s)	85, 1681
Vha464I	CTTAAG	1 cut(s)	2536
VpaK11BI	GGWCC	1 cut(s)	701
VspI	ATTAAT	2 cut(s)	927, 2309
XapI	RAATTY	7 cut(s)	448, 489, 1193, 1356, 1739, 1833, 2527
XceI	RCATGY	3 cut(s)	772, 1718, 2690
XmnI	GAANNNNTTC	2 cut(s)	685, 1196
XspI	CTAG	5 cut(s)	434, 1316, 2243, 2337, 2861
ZraI	GACGTC	1 cut(s)	391
ZrmI	AGTACT	2 cut(s)	659, 736
Zsp2I	ATGCAT	2 cut(s)	994, 2203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.