Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009188

Physical Location & Seq

Genomic Coordinates Reverse (-)

4322761 .. 4333186

Gene Structure 10426 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1025200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGGTGGAATTGGGATTGTTGTCCGGAAGCAGCAGCAAGTATAGTAGTTGTAAGCCGGTGAATGTGAGCTTCAGGATACCGTATTACACTGAGTGGGGTCAAAGCCTGCTGGTGTGCGGCTCCGAGCCGGTGCTTGGTTCATGGAATATCAAAAAGGGTCTGCAACTGAGCCCTGTCCACCATGGGAAGGAGCTCATATGGTTTGGGAACATCTCTGTTCCAAGTGGCTTCAAATGTGACTACACTTACTATGTCGTCGATGAGAAGCGAAACGTTCTGAGATGGGAAATGGGAGATAAGCGTAAAGTATTATTGCCTGAGGGGATCCAAGATGCAGAGGCAGTGGAGCTTCATGACCTTTGGCAGGTGGGTGCCGATTCACTTCCTTTTAGAAGTGCGTTCAAAGACGTCATCTTTGATTCGAAATTCAGTTTGGACATTGAAGTACCTCCTGGAGTCATCCAAAACACTTTGGACCAGGATGATTCTGTTCTTGTCCATTTCAAAATCAGCTGTCCAAACATAGAAGAAGAAACAGCGATATTTATTATTGGTAACACCTCAAAGTTGGGACAGTGGAATGTTCAGAATGGGCTTAAACTAAGCTACGCTGGTGAATCCATTTGGCATGCTGACTGTGTTTTGCCGAAGAGAAACTTTTCTCCAGAAAATGGTCCAAACCGTGACCTTGTTCTTGACTCCTCAAAAACTCAACCGAGATACATTTTTCTTTCAGATGGCATGTTGCGAGAAATGCCTTGGCGGGGTGCTGGTGTTGCAATCCCAATGTTTTCTGTTAGATCAGAAAATGATCTTGGAGTTGGAGAGTTTCTTGACCTGAAATTAGTTGTTGACTGGGCTGCAGATTCTGGCTTTCATTTGGTTCAGCTTTTACCTATTAATGATACATCAGTGCATGGAATGTGGTGGGATTCATACCCCTACAGCTCACTTTCTGTATTTGCATTGCATCCTTTATACCTGAGAGTACAGGAACTTTCAGAAAGTATGTCTTCGGATATCAAGCTCGAGATTGAGAAGGCAAAAGAACAATTGGATGGAAAGGATGTTGATTATGAGGCTACCTTGACTACCAAACTTTCTATTGCGAATAAAGTATTTGCTCAAGAGAAGGATTTGATTCTTAACTCCAGTTCCTTCCAGAATTTTTTTTCTGAGAATCAGGATTGGCTAAAACCCTATGCGGCCTTCTGTTTTTTACGGGACTTCTTTGAAACATCAGATCACAGTCAATGGGGTCGGTTTTCTCATTTTTCAAAAGAAAAGCTAGAGAAACTCAGCTCCAAAGACAGCTGTCACTATGACATAATCTGCTTCCATTATTATGTCCAATTTCATTTGTATGGACAACTCTCAGAAGCTGCAGATTATGCAAGAAGGAAAGGTGTAATATTGAAAGGAGATCTACCTATTGGTGTTGACAGAAACAGTGTGGATACGTGGGTTAATCCAAATTTGTTCCGCATGAACACATCCACTGGAGCTCCTCCAGATTATTTTGATAAAAATGGGCAGAATTGGGGTTTCCCTACCTACAATTGGGAGGAGATGTCGAAAGACAACTATGCATGGTGGCGTGCTCGTTTGACACAGATGGCAAAATACTTTACCGCTTACAGAATTGATCACATTTTGGGTTTCTTTCGTATCTGGGAGCTCCCAGAACATGCCATGACTGGTCTTGTTGGGAAATTTCGACCTTCCATTCCACTAAGTCAGGAGGAACTTGAAAGAGAGGGCATATGGGATTTTGATCGCTTGAGTCGCCCATATATTCTGCAGGAATATTTACAGGATAAATTTGGAGCTTCATGGACTTTTATTGCCTCAAATTTTCTAAATGAATATCAGAAGAACCGCTATGAGTTTAAGGAGGACTGCAACACTCAGAAAAAAATTGCTTCCAAGCTGAAATCATTTGCAGAAAGGTCTCTGTTGCAGGATGAAGAGAAGATACGGCATGAACTCTTTGATCTTATACAGAACATAGTTCTCATCAGAGATCCAGAAAATCCAAGGAATTTCTATCCTCGTTTCAATCTTGAAGATACACCAAGTTTTAATGATTTGGACGACCACAGCAAAAACGTATTGAAAAGATTATACTATGATTACTACTTCCACCGGCAAGAAAATCTTTGGCGGGAGAATGCTTTGAAAACGTTACCTGCTCTGCTTAACTCATCAGATATGCTTGCCTGTGGGGAAGATCTGGGGCTTATTCCTTCTTGTGTTCATCCTGTGATGCAAGAACTAGGATTAATAGGTTTACGCATTCAGCGCATGCCCAGTGAACCTGATTTGGAGTTTGGTATTCCTTCTCAATATGGCTATATGACAGTATGTGCTCCGTCATGCCATGACTGCTCAACCTTGCGTGCTTGGTGGGAAGAAGATGAAGAAAGAAGGCAGCGATATTTTAAGAATGTGGTGGGGTCTGACATGTCACCACCTGCTCGATGTGTTCCAGAGATTGCACATTTCATCTTAAGGCAACATGTTGAAGCACCATCAATGTGGGCCATTTTTCCGCTTCAGGATTTGTTAGCGTTGAAAGAAGAATACACAACACGCCCTGCAAAAGAGGAGACGATCAATGACCCCACAAATCCCAAACACTATTGGAGATACCGTGTACATGTGACAATGGAGGCTTTGATCAAGGATAATGAACTCATATCGACCATCAAGGATCTTGTCCGTTTGAGTGGAAGATCATATCCGGCAGTACAAGCTGAAAAACAACCAAGTCAGGAATCAGCAGCAGTTGCAGGTACAGAGAAGCAGAAAATGTCCAGCAGCAAAGATAAGGTTCACCCGGCAGCGTCGTTGAACGGAGTTCCACAGGAGACCGTGGCTGTTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000023 GO:0000025 GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0004133 GO:0004134 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0005984 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009987 GO:0010297 GO:0015980 GO:0016052 GO:0016740 GO:0016757 GO:0016758 GO:0030246 GO:0030247 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0055114 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

CBM_20 Glyco_hydro_77

Protein Analysis

963

Amino Acids

110.95

Weight (kDa)

5.49

Isoelectric Point (pI)

48.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	20 - 112	8e-22	Starch binding domain
CBM_20	PF00686	164 - 217	3.1e-07	Starch binding domain
Glyco_hydro_77	PF02446	261 - 888	5.7e-145	4-alpha-glucanotransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40840
fragaria_vesca	FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260 FvH4_1g29260
malus_domestica	MD00G1025200.v1.1
prunus_persica	Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1 Prupe.7G019400_v2.0.a1
pyrus_communis	pycom12g09050
rosa_chinensis	RchiOBHm_Chr3g0494731
rosa_laevigata	RLG00000022663
rosa_multiflora	Rmu_sc0000198.1_g000016
rosa_roxburghii	Rroxscaffold_6G00390950
rosa_rugosa	Rorug03G0268100
rosa_samantha	Rh3AG316600 Rh3BG351900 Rh3CG349000 Rh3DG351500
rosa_wichuraiana	Rw3G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	355, 2482
AatII	GACGTC	1 cut(s)	411
Acc36I	ACCTGC	4 cut(s)	355, 2197, 2482, 2783
AccB1I	GGYRCC	1 cut(s)	371
AccB7I	CCANNNNNTGG	1 cut(s)	671
AccIII	TCCGGA	1 cut(s)	23
AciI	CCGC	8 cut(s)	117, 763, 1205, 1483, 1632, 1879, 2164, 2552
AclI	AACGTT	2 cut(s)	273, 2183
AclWI	GGATC	4 cut(s)	319, 332, 2018, 2721
AcsI	RAATTY	7 cut(s)	425, 1165, 1474, 1712, 1820, 1852, 2041
AcuI	CTGAAG	2 cut(s)	55, 2540
AcyI	GRCGYC	1 cut(s)	408
AdeI	CACNNNGTG	1 cut(s)	93
AfaI	GTAC	5 cut(s)	447, 990, 2658, 2751, 2797
AfiI	CCNNNNNNNGG	6 cut(s)	134, 187, 364, 671, 764, 1560
AflII	CTTAAG	1 cut(s)	2509
AflIII	ACRYGT	3 cut(s)	2463, 2518, 2659
AjnI	CCWGG	2 cut(s)	451, 477
AjuI	GAANNNNNNNTTGG	4 cut(s)	416, 448, 798, 830
Alw21I	GWGCWC	5 cut(s)	195, 1507, 1603, 1680, 2371
Alw26I	GTCTC	3 cut(s)	1956, 2603, 2864
AlwI	GGATC	4 cut(s)	319, 332, 2018, 2721
AlwNI	CAGNNNCTG	2 cut(s)	869, 1382
Ama87I	CYCGRG	1 cut(s)	1028
Aor13HI	TCCGGA	1 cut(s)	23
AoxI	GGCC	2 cut(s)	1206, 2541
ApeKI	GCWGC	8 cut(s)	30, 33, 860, 1382, 2431, 2783, 2820, 2843
ApoI	RAATTY	7 cut(s)	425, 1165, 1474, 1712, 1820, 1852, 2041
ArsI	GACNNNNNNTTYG	2 cut(s)	398, 430
AseI	ATTAAT	2 cut(s)	900, 2282
Asp700I	GAANNNNTTC	1 cut(s)	658
AspLEI	GCGC	1 cut(s)	2304
AspS9I	GGNCC	3 cut(s)	475, 674, 2541
AsuC2I	CCSGG	1 cut(s)	2840
AsuHPI	GGTGA	4 cut(s)	70, 626, 2460, 2828
AsuII	TTCGAA	1 cut(s)	422
AvaI	CYCGRG	1 cut(s)	1028
AvaII	GGWCC	2 cut(s)	475, 674
AxyI	CCTNAGG	1 cut(s)	318
BaeI	ACNNNNGTAYC	4 cut(s)	897, 930, 1449, 1482
BamHI	GGATCC	1 cut(s)	324
BanI	GGYRCC	1 cut(s)	371
BanII	GRGCYC	4 cut(s)	173, 195, 1507, 1680
BarI	GAAGNNNNNNTAC	2 cut(s)	1959, 1991
BbsI	GAAGAC	1 cut(s)	1005
Bbv12I	GWGCWC	5 cut(s)	195, 1507, 1603, 1680, 2371
BbvI	GCAGC	8 cut(s)	42, 45, 847, 1369, 2443, 2795, 2832, 2855
BccI	CCATC	6 cut(s)	276, 731, 1052, 1609, 2539, 2714
BceAI	ACGGC	1 cut(s)	1994
BciT130I	CCWGG	2 cut(s)	453, 479
BciVI	GTATCC	2 cut(s)	69, 1450
BclI	TGATCA	2 cut(s)	1645, 2679
BcnI	CCSGG	1 cut(s)	2840
BcoDI	GTCTC	3 cut(s)	1956, 2603, 2864
BfaI	CTAG	2 cut(s)	1289, 2276
BfmI	CTRYAG	4 cut(s)	861, 943, 1383, 1799
BfrI	CTTAAG	1 cut(s)	2509
BfuAI	ACCTGC	4 cut(s)	355, 2197, 2482, 2783
BfuI	GTATCC	2 cut(s)	69, 1450
BglII	AGATCT	2 cut(s)	1423, 2230
Bme1390I	CCNGG	3 cut(s)	453, 479, 2840
Bme18I	GGWCC	2 cut(s)	475, 674
BmeT110I	CYCGRG	1 cut(s)	1028
BmgT120I	GGNCC	3 cut(s)	475, 674, 2541
BmiI	GGNNCC	3 cut(s)	121, 326, 373
BmrFI	CCNGG	3 cut(s)	453, 479, 2840
BmrI	ACTGGG	2 cut(s)	865, 2304
BmsI	GCATC	3 cut(s)	322, 979, 2256
BmuI	ACTGGG	2 cut(s)	865, 2304
BoxI	GACNNNNGTC	1 cut(s)	1314
BpiI	GAAGAC	1 cut(s)	1005
BpmI	CTGGAG	5 cut(s)	474, 648, 1135, 1494, 1521
Bpu14I	TTCGAA	1 cut(s)	422
BpuEI	CTTGAG	2 cut(s)	1110, 1801
BpuMI	CCSGG	1 cut(s)	2840
BsaAI	YACGTR	1 cut(s)	1461
BsaBI	GATNNNNATC	1 cut(s)	1773
BsaHI	GRCGYC	1 cut(s)	408
BsaI	GGTCTC	2 cut(s)	1956, 2864
BsaJI	CCNNGG	4 cut(s)	181, 758, 2036, 2874
BsaWI	WCCGGW	1 cut(s)	23
BsaXI	ACNNNNNCTCC	6 cut(s)	1489, 1519, 1556, 1586, 2638, 2668
Bsc4I	CCNNNNNNNGG	6 cut(s)	134, 187, 364, 671, 764, 1560
Bse118I	RCCGGY	3 cut(s)	55, 127, 2145
Bse1I	ACTGG	5 cut(s)	860, 1152, 1504, 1702, 2310
Bse21I	CCTNAGG	1 cut(s)	318
Bse3DI	GCAATG	1 cut(s)	965
Bse8I	GATNNNNATC	1 cut(s)	1773
BseAI	TCCGGA	1 cut(s)	23
BseBI	CCWGG	2 cut(s)	453, 479
BseDI	CCNNGG	4 cut(s)	181, 758, 2036, 2874
BseGI	GGATG	8 cut(s)	459, 487, 970, 1063, 1072, 1493, 1969, 2257
BseJI	GATNNNNATC	1 cut(s)	1773
BseLI	CCNNNNNNNGG	6 cut(s)	134, 187, 364, 671, 764, 1560
BseMI	GCAATG	1 cut(s)	965
BseMII	CTCAG	9 cut(s)	81, 158, 269, 309, 974, 1167, 1312, 1389, 1922
BseNI	ACTGG	5 cut(s)	860, 1152, 1504, 1702, 2310
BseRI	GAGGAG	4 cut(s)	691, 1497, 1580, 2621
BseXI	GCAGC	8 cut(s)	42, 45, 847, 1369, 2443, 2795, 2832, 2855
BshFI	GGCC	2 cut(s)	1208, 2543
BshNI	GGYRCC	1 cut(s)	371
BsiHKAI	GWGCWC	5 cut(s)	195, 1507, 1603, 1680, 2371
BsiHKCI	CYCGRG	1 cut(s)	1028
BsiSI	CCGG	6 cut(s)	24, 56, 128, 2146, 2744, 2840
BslFI	GGGAC	2 cut(s)	585, 1238
BslI	CCNNNNNNNGG	6 cut(s)	134, 187, 364, 671, 764, 1560
BsmAI	GTCTC	3 cut(s)	1956, 2603, 2864
BsmBI	CGTCTC	1 cut(s)	2603
BsmFI	GGGAC	2 cut(s)	585, 1238
BsmI	GAATGC	2 cut(s)	2176, 2295
BsnI	GGCC	2 cut(s)	1208, 2543
Bso31I	GGTCTC	2 cut(s)	1956, 2864
BsoBI	CYCGRG	1 cut(s)	1028
Bsp119I	TTCGAA	1 cut(s)	422
Bsp1286I	GDGCHC	6 cut(s)	173, 195, 1507, 1603, 1680, 2371
Bsp13I	TCCGGA	1 cut(s)	23
Bsp1407I	TGTACA	1 cut(s)	2656
Bsp19I	CCATGG	1 cut(s)	181
BspACI	CCGC	8 cut(s)	117, 763, 1205, 1483, 1632, 1879, 2164, 2552
BspANI	GGCC	2 cut(s)	1208, 2543
BspCNI	CTCAG	9 cut(s)	82, 159, 270, 310, 975, 1168, 1311, 1388, 1921
BspEI	TCCGGA	1 cut(s)	23
BspHI	TCATGA	1 cut(s)	352
BspLI	GGNNCC	3 cut(s)	121, 326, 373
BspMAI	CTGCAG	3 cut(s)	865, 1387, 1803
BspMI	ACCTGC	4 cut(s)	355, 2197, 2482, 2783
BspPI	GGATC	4 cut(s)	319, 332, 2018, 2721
BspT104I	TTCGAA	1 cut(s)	422
BspT107I	GGYRCC	1 cut(s)	371
BspTI	CTTAAG	1 cut(s)	2509
BspTNI	GGTCTC	2 cut(s)	1956, 2864
BsrDI	GCAATG	1 cut(s)	965
BsrFI	RCCGGY	3 cut(s)	55, 127, 2145
BsrGI	TGTACA	1 cut(s)	2656
BsrI	ACTGG	5 cut(s)	860, 1152, 1504, 1702, 2310
BssAI	RCCGGY	3 cut(s)	55, 127, 2145
BssECI	CCNNGG	4 cut(s)	181, 758, 2036, 2874
BssNI	GRCGYC	1 cut(s)	408
BssT1I	CCWWGG	3 cut(s)	181, 758, 2036
Bst2UI	CCWGG	2 cut(s)	453, 479
Bst4CI	ACNGT	9 cut(s)	81, 576, 638, 683, 1250, 1451, 2362, 2654, 2875
Bst6I	CTCTTC	2 cut(s)	644, 1962
BstACI	GRCGYC	1 cut(s)	408
BstAFI	CTTAAG	1 cut(s)	2509
BstAPI	GCANNNNNTGC	2 cut(s)	1391, 2789
BstAUI	TGTACA	1 cut(s)	2656
BstBAI	YACGTR	1 cut(s)	1461
BstBI	TTCGAA	1 cut(s)	422
BstC8I	GCNNGC	6 cut(s)	107, 630, 1599, 2217, 2306, 2400
BstDSI	CCRYGG	2 cut(s)	181, 2874
BstENI	CCTNNNNNAGG	1 cut(s)	362
BstF5I	GGATG	8 cut(s)	459, 487, 970, 1063, 1072, 1493, 1969, 2257
BstHHI	GCGC	1 cut(s)	2304
BstMAI	GTCTC	3 cut(s)	1956, 2603, 2864
BstMWI	GCNNNNNNNGC	8 cut(s)	754, 776, 1391, 1785, 2301, 2385, 2753, 2789
BstNI	CCWGG	2 cut(s)	453, 479
BstNSI	RCATGY	7 cut(s)	632, 745, 1691, 2308, 2467, 2522, 2663
BstPAI	GACNNNNGTC	1 cut(s)	1314
BstSCI	CCNGG	3 cut(s)	451, 477, 2838
BstSFI	CTRYAG	4 cut(s)	861, 943, 1383, 1799
BstV1I	GCAGC	8 cut(s)	42, 45, 847, 1369, 2443, 2795, 2832, 2855
BstV2I	GAAGAC	1 cut(s)	1005
BstX2I	RGATCY	5 cut(s)	324, 1423, 2023, 2230, 2713
BstXI	CCANNNNNNTGG	1 cut(s)	2538
BstYI	RGATCY	5 cut(s)	324, 1423, 2023, 2230, 2713
Bsu36I	CCTNAGG	1 cut(s)	318
BsuI	GTATCC	2 cut(s)	69, 1450
BsuRI	GGCC	2 cut(s)	1208, 2543
BtgI	CCRYGG	2 cut(s)	181, 2874
BtsCI	GGATG	8 cut(s)	459, 487, 970, 1063, 1072, 1493, 1969, 2257
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	7 cut(s)	87, 348, 581, 918, 1456, 1497, 2317
BveI	ACCTGC	4 cut(s)	355, 2197, 2482, 2783
Cac8I	GCNNGC	6 cut(s)	107, 630, 1599, 2217, 2306, 2400
CaiI	CAGNNNCTG	2 cut(s)	869, 1382
CciI	TCATGA	1 cut(s)	352
CfoI	GCGC	1 cut(s)	2304
Cfr10I	RCCGGY	3 cut(s)	55, 127, 2145
Cfr13I	GGNCC	3 cut(s)	475, 674, 2541
CseI	GACGC	1 cut(s)	2835
Csp6I	GTAC	5 cut(s)	446, 989, 2657, 2750, 2796
CviQI	GTAC	5 cut(s)	446, 989, 2657, 2750, 2796
DraIII	CACNNNGTG	1 cut(s)	93
Eam1104I	CTCTTC	2 cut(s)	644, 1962
EarI	CTCTTC	2 cut(s)	644, 1962
Ecl136II	GAGCTC	3 cut(s)	193, 1505, 1678
Eco130I	CCWWGG	3 cut(s)	181, 758, 2036
Eco24I	GRGCYC	4 cut(s)	173, 195, 1507, 1680
Eco31I	GGTCTC	2 cut(s)	1956, 2864
Eco32I	GATATC	1 cut(s)	1021
Eco47I	GGWCC	2 cut(s)	475, 674
Eco53kI	GAGCTC	3 cut(s)	193, 1505, 1678
Eco57I	CTGAAG	2 cut(s)	55, 2540
Eco81I	CCTNAGG	1 cut(s)	318
Eco88I	CYCGRG	1 cut(s)	1028
EcoICRI	GAGCTC	3 cut(s)	193, 1505, 1678
EcoNI	CCTNNNNNAGG	1 cut(s)	362
EcoRII	CCWGG	2 cut(s)	451, 477
EcoRV	GATATC	1 cut(s)	1021
EcoT14I	CCWWGG	3 cut(s)	181, 758, 2036
EcoT22I	ATGCAT	1 cut(s)	1591
EcoT38I	GRGCYC	4 cut(s)	173, 195, 1507, 1680
ErhI	CCWWGG	3 cut(s)	181, 758, 2036
Esp3I	CGTCTC	1 cut(s)	2603
FalI	AAGNNNNNCTT	6 cut(s)	641, 673, 997, 1029, 2142, 2174
FaqI	GGGAC	2 cut(s)	585, 1238
FauI	CCCGC	2 cut(s)	756, 2157
FauNDI	CATATG	2 cut(s)	197, 1763
FbaI	TGATCA	2 cut(s)	1645, 2679
FokI	GGATG	8 cut(s)	446, 494, 957, 1070, 1079, 1480, 1976, 2244
FriOI	GRGCYC	4 cut(s)	173, 195, 1507, 1680
FspBI	CTAG	2 cut(s)	1289, 2276
GlaI	GCGC	1 cut(s)	2303
GsuI	CTGGAG	5 cut(s)	474, 648, 1135, 1494, 1521
HaeIII	GGCC	2 cut(s)	1208, 2543
HapII	CCGG	6 cut(s)	24, 56, 128, 2146, 2744, 2840
HgaI	GACGC	1 cut(s)	2835
HhaI	GCGC	1 cut(s)	2304
Hin1I	GRCGYC	1 cut(s)	408
Hin6I	GCGC	1 cut(s)	2302
HinP1I	GCGC	1 cut(s)	2302
HincII	GTYRAC	2 cut(s)	853, 1441
HindII	GTYRAC	2 cut(s)	853, 1441
HpaII	CCGG	6 cut(s)	24, 56, 128, 2146, 2744, 2840
HphI	GGTGA	4 cut(s)	70, 626, 2460, 2828
Hpy166II	GTNNAC	7 cut(s)	178, 853, 1441, 2291, 2315, 2657, 2836
Hpy8I	GTNNAC	7 cut(s)	178, 853, 1441, 2291, 2315, 2657, 2836
Hpy99I	CGWCG	2 cut(s)	260, 2851
HpyCH4III	ACNGT	9 cut(s)	81, 576, 638, 683, 1250, 1451, 2362, 2654, 2875
HpyCH4IV	ACGT	5 cut(s)	273, 408, 1460, 2109, 2183
HpyF10VI	GCNNNNNNNGC	8 cut(s)	754, 776, 1391, 1785, 2301, 2385, 2753, 2789
HpySE526I	ACGT	5 cut(s)	273, 408, 1460, 2109, 2183
Hsp92I	GRCGYC	1 cut(s)	408
HspAI	GCGC	1 cut(s)	2302
Kpn2I	TCCGGA	1 cut(s)	23
Ksp22I	TGATCA	2 cut(s)	1645, 2679
Lsp1109I	GCAGC	8 cut(s)	42, 45, 847, 1369, 2443, 2795, 2832, 2855
LweI	GCATC	3 cut(s)	322, 979, 2256
MaeI	CTAG	2 cut(s)	1289, 2276
MaeII	ACGT	5 cut(s)	273, 408, 1460, 2109, 2183
MaeIII	GTNAC	7 cut(s)	236, 554, 683, 1316, 2184, 2466, 2662
MfeI	CAATTG	2 cut(s)	1052, 1558
MflI	RGATCY	5 cut(s)	324, 1423, 2023, 2230, 2713
MhlI	GDGCHC	6 cut(s)	173, 195, 1507, 1603, 1680, 2371
MlyI	GAGTC	3 cut(s)	465, 692, 1792
MmeI	TCCRAC	1 cut(s)	802
Mph1103I	ATGCAT	1 cut(s)	1591
MroI	TCCGGA	1 cut(s)	23
MroXI	GAANNNNTTC	1 cut(s)	658
MslI	CAYNNNNRTG	3 cut(s)	1344, 1362, 2536
MspA1I	CMGCKG	2 cut(s)	513, 1314
MspCI	CTTAAG	1 cut(s)	2509
MspI	CCGG	6 cut(s)	24, 56, 128, 2146, 2744, 2840
MspR9I	CCNGG	3 cut(s)	453, 479, 2840
MunI	CAATTG	2 cut(s)	1052, 1558
Mva1269I	GAATGC	2 cut(s)	2176, 2295
MvaI	CCWGG	2 cut(s)	453, 479
MwoI	GCNNNNNNNGC	8 cut(s)	754, 776, 1391, 1785, 2301, 2385, 2753, 2789
NciI	CCSGG	1 cut(s)	2840
NcoI	CCATGG	1 cut(s)	181
NdeI	CATATG	2 cut(s)	197, 1763
NlaIV	GGNNCC	3 cut(s)	121, 326, 373
NmuCI	GTSAC	5 cut(s)	236, 683, 1316, 2466, 2662
NsiI	ATGCAT	1 cut(s)	1591
NspI	RCATGY	7 cut(s)	632, 745, 1691, 2308, 2467, 2522, 2663
NspV	TTCGAA	1 cut(s)	422
PaeI	GCATGC	2 cut(s)	632, 2308
PaeR7I	CTCGAG	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	352
PaqCI	CACCTGC	2 cut(s)	355, 2482
PciI	ACATGT	3 cut(s)	2463, 2518, 2659
PctI	GAATGC	2 cut(s)	2176, 2295
PdmI	GAANNNNTTC	1 cut(s)	658
PfeI	GAWTC	9 cut(s)	377, 419, 485, 617, 866, 932, 1141, 1180, 2777
PflMI	CCANNNNNTGG	1 cut(s)	671
PfoI	TCCNGGA	1 cut(s)	451
PleI	GAGTC	3 cut(s)	464, 692, 1791
PpsI	GAGTC	3 cut(s)	464, 692, 1791
Ppu21I	YACGTR	1 cut(s)	1461
PscI	ACATGT	3 cut(s)	2463, 2518, 2659
PshAI	GACNNNNGTC	1 cut(s)	1314
PshBI	ATTAAT	2 cut(s)	900, 2282
Psp124BI	GAGCTC	3 cut(s)	195, 1507, 1680
Psp1406I	AACGTT	2 cut(s)	273, 2183
Psp6I	CCWGG	2 cut(s)	451, 477
PspGI	CCWGG	2 cut(s)	451, 477
PspN4I	GGNNCC	3 cut(s)	121, 326, 373
PspPI	GGNCC	3 cut(s)	475, 674, 2541
PstI	CTGCAG	3 cut(s)	865, 1387, 1803
PstNI	CAGNNNCTG	2 cut(s)	869, 1382
PsuI	RGATCY	5 cut(s)	324, 1423, 2023, 2230, 2713
PvuII	CAGCTG	2 cut(s)	513, 1314
RsaI	GTAC	5 cut(s)	447, 990, 2658, 2751, 2797
RsaNI	GTAC	5 cut(s)	446, 989, 2657, 2750, 2796
RseI	CAYNNNNRTG	3 cut(s)	1344, 1362, 2536
SacI	GAGCTC	3 cut(s)	195, 1507, 1680
Sau96I	GGNCC	3 cut(s)	475, 674, 2541
SchI	GAGTC	3 cut(s)	465, 692, 1792
ScrFI	CCNGG	3 cut(s)	453, 479, 2840
SduI	GDGCHC	6 cut(s)	173, 195, 1507, 1603, 1680, 2371
SfaNI	GCATC	3 cut(s)	322, 979, 2256
SfcI	CTRYAG	4 cut(s)	861, 943, 1383, 1799
Sfr274I	CTCGAG	1 cut(s)	1028
SfuI	TTCGAA	1 cut(s)	422
SinI	GGWCC	2 cut(s)	475, 674
SlaI	CTCGAG	1 cut(s)	1028
SmiMI	CAYNNNNRTG	3 cut(s)	1344, 1362, 2536
SmlI	CTYRAG	4 cut(s)	1028, 1125, 1780, 2509
SmoI	CTYRAG	4 cut(s)	1028, 1125, 1780, 2509
SphI	GCATGC	2 cut(s)	632, 2308
SsiI	CCGC	8 cut(s)	117, 763, 1205, 1483, 1632, 1879, 2164, 2552
SspI	AATATT	2 cut(s)	1413, 1808
SspMI	CTAG	2 cut(s)	1289, 2276
SstI	GAGCTC	3 cut(s)	195, 1507, 1680
StyD4I	CCNGG	3 cut(s)	451, 477, 2838
StyI	CCWWGG	3 cut(s)	181, 758, 2036
TaaI	ACNGT	9 cut(s)	81, 576, 638, 683, 1250, 1451, 2362, 2654, 2875
TaiI	ACGT	5 cut(s)	276, 411, 1463, 2112, 2186
TaqI	TCGA	7 cut(s)	258, 422, 1029, 1574, 1717, 2479, 2702
TatI	WGTACW	3 cut(s)	988, 2656, 2749
TauI	GCSGC	2 cut(s)	120, 1208
TfiI	GAWTC	9 cut(s)	377, 419, 485, 617, 866, 932, 1141, 1180, 2777
TscAI	CASTG	7 cut(s)	94, 348, 581, 918, 1456, 1504, 2317
TseFI	GTSAC	5 cut(s)	236, 683, 1316, 2466, 2662
TseI	GCWGC	8 cut(s)	30, 33, 860, 1382, 2431, 2783, 2820, 2843
Tsp45I	GTSAC	5 cut(s)	236, 683, 1316, 2466, 2662
TspGWI	ACGGA	3 cut(s)	2361, 2711, 2871
TspRI	CASTG	7 cut(s)	94, 348, 581, 918, 1456, 1504, 2317
Van91I	CCANNNNNTGG	1 cut(s)	671
Vha464I	CTTAAG	1 cut(s)	2509
VpaK11BI	GGWCC	2 cut(s)	475, 674
VspI	ATTAAT	2 cut(s)	900, 2282
XagI	CCTNNNNNAGG	1 cut(s)	362
XapI	RAATTY	7 cut(s)	425, 1165, 1474, 1712, 1820, 1852, 2041
XceI	RCATGY	7 cut(s)	632, 745, 1691, 2308, 2467, 2522, 2663
XhoI	CTCGAG	1 cut(s)	1028
XmnI	GAANNNNTTC	1 cut(s)	658
XspI	CTAG	2 cut(s)	1289, 2276
ZraI	GACGTC	1 cut(s)	409
Zsp2I	ATGCAT	1 cut(s)	1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.