FvH4_2g02001

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015238
Physical Location & Seq
Forward (+)
1697082 .. 1702581
5500 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02001.t3

Sequence Viewer

Length: 1482 bp
ATGGCAGCAATTCAAATCTTCAATACCTCTCGTCCTTTCCCAACCACAAAACCCAAACCCCTTTTCTTCCTCTGCTACTCCTCCTTTTCTTCCTCACCGTCCGACGAAAGTCCATCAGAGCCCAACCCTCTAATCCCATCCGTCGTCTCTATCCTAACCCAGCTCCGATCCAAGTCCCGTTGGGGCTACCTCCGCACTTTATATCCTTCCGGCTTCACCCCAAACGACTTTTCTCAAATCTCCCTCCAAATAAAAAACAATCCTCATCTCGTCCTTCGCTTCTTCCAGTGGACCCAGAACAAGAACAACTCTCTCTGCGCCCACAACCTCCTCTCCTACTCTACCATCATCCACATCCTCGCCCGATCTCGCCTCAAATCCCAAGCTTATTCCCTTATTGGAGACGCCATTTGGGTCTGGGAGCCGTTGGAAGTGTTTGAAACTCTGGTCAAGACTTACCGACAATGTGGGTCGGCCCCATTTGTGTTTAATTATCTGATCAAAGCTTGCTTGGAGTCGAAGAAAATTGACCCGGCAATACAGATTGTTAGAATGATACTCAGTCGCGGGATTAGTCCTGGTTTGAGCACCTGCAATAGTTTGATTCGGTGCGTAATGCAGCGTCAAGGTGCGTATGCAGGTTATGAGATTTATAGAGAGGTTTTTGGATTGGATGGTAGGGTCTTAGACGATAATGCAAAAAGGGTTGTGAGAACTAGTCCCAATGTGCAAACCTTCAATGAATTGATGTTGGGGTTTTATCAGGATGGGGCAGTAGAGATGGTGAAGGAGATTTGGGACCAAATGGCGGACTTCAGTTGCTGTCCTGATGTTTATAGTTATTGTATTTTGATGGAGACTTATTGTGAGGATGAGACAATGAGTAAAGCGGAGGAGTTGTGGGAAGAAATGAGAGCAAAGGGTGTGGAGCCTGATGCTGTTGCTTATAACACTATGATTGGAGGATTTTGCAAAGTTGGAAAGATGGAAATGGCTGAAGAGTTTTTCAAACAAATGGGATTGAGTGGAATAGAGAGTACTAACGCCACTTGTGAGCAACTTGTTAGAGGGTACTGTAAAATTGGAAAAATTGATTCAGCAATACTAGTGTATAAGGATATGCTTAGGAAAAATTTCAGGGCTGAGAGTTTAACTGTAGAAGAGTTGATTAGAGGGCTTTGTGATGAGAATAGGGTTTTGGAAGCCTTGGAAGTTATGCGAGCTGCAATGGTAGATTATGGTTTCTGTCCAAGAGAGAAGTCGTATGAGTTTTTGATAAGAGGGTTGTGTGAGCAGGGAAAGTTGGAAGAAGCACTTAAGCTTCAGGCTCAGATGGTGGGAAAAGGGTTTAAACCGAATTCTGAAATCTATGGTGCATTTATTAGTGGCTATATGAAAGAAGGAAACATGGAAGTAGCAGAATTGTTGAGGAATGAAATGTTGGATACGGAGCTGTGTGGGATGGGGATAAATAATCAATGA

Protein Analysis

494

Amino Acids

55.98

Weight (kDa)

5.52

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 241 - 289 2.1e-09 PPR repeat family
PPR_1 PF12854 273 - 305 1.6e-06 PPR repeat
PPR_2 PF13041 283 - 316 3.8e-06 PPR repeat family
PPR_1 PF12854 307 - 339 1.5e-11 PPR repeat
PPR_2 PF13041 311 - 360 6.8e-14 PPR repeat family
PPR PF01535 314 - 344 1e-08 PPR repeat
PPR_2 PF13041 354 - 395 1.7e-06 PPR repeat family
PPR_2 PF13041 421 - 454 9.8e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15980
fragaria_vesca FvH4_2g02001 FvH4_2g02001 FvH4_2g02001
malus_domestica MD10G1084800.v1.1
prunus_persica Prupe.8G107700_v2.0.a1
pyrus_communis pycom10g06650
rosa_chinensis RchiOBHm_Chr6g0245191
rosa_laevigata RLG00000015317
rosa_multiflora Rmu_sc0027529.1_g000005 Rmu_sc0038123.1_g000001
rosa_roxburghii Rroxscaffold_7G00215370
rosa_rugosa Rorug05G0512900
rosa_samantha Rh6AG027800 Rh6BG023900
rosa_wichuraiana Rw6G002350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AarI CACCTGC 1 cut(s) 599
Acc36I ACCTGC 2 cut(s) 599, 629
AccII CGCG 1 cut(s) 567
AciI CCGC 4 cut(s) 193, 567, 809, 890
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 2 cut(s) 1132, 1357
AcuI CTGAAG 3 cut(s) 799, 1017, 1307
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 2 cut(s) 1039, 1073
AfiI CCNNNNNNNGG 1 cut(s) 808
AflII CTTAAG 1 cut(s) 1316
AgsI TTSAA 5 cut(s) 14, 22, 440, 739, 1009
AhlI ACTAGT 2 cut(s) 716, 1105
AjnI CCWGG 1 cut(s) 577
AjuI GAANNNNNNNTTGG 8 cut(s) 47, 79, 214, 246, 1181, 1213, 1424, 1456
AluBI AGCT 6 cut(s) 163, 386, 506, 1223, 1321, 1453
AluI AGCT 6 cut(s) 163, 386, 506, 1223, 1321, 1453
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 4 cut(s) 151, 396, 851, 869
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 822
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 3 cut(s) 5, 619, 1223
ApoI RAATTY 2 cut(s) 1132, 1357
Asp700I GAANNNNTTC 1 cut(s) 1133
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 3 cut(s) 291, 475, 799
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 3 cut(s) 87, 208, 796
AvaII GGWCC 2 cut(s) 291, 799
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 590
BbvI GCAGC 3 cut(s) 17, 631, 1210
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 579
BciVI GTATCC 1 cut(s) 1438
BclI TGATCA 1 cut(s) 498
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 4 cut(s) 151, 396, 851, 869
BcuI ACTAGT 2 cut(s) 716, 1105
BfaI CTAG 2 cut(s) 717, 1106
BfmI CTRYAG 1 cut(s) 1155
BfrI CTTAAG 1 cut(s) 1316
BfuAI ACCTGC 2 cut(s) 599, 629
BfuI GTATCC 1 cut(s) 1438
BisI GCNGC 3 cut(s) 6, 620, 1224
BlsI GCNGC 3 cut(s) 7, 621, 1225
BmcAI AGTACT 1 cut(s) 1039
Bme1390I CCNGG 2 cut(s) 533, 579
Bme18I GGWCC 2 cut(s) 291, 799
BmgT120I GGNCC 3 cut(s) 291, 475, 799
BmiI GGNNCC 5 cut(s) 293, 423, 477, 800, 930
BmrFI CCNGG 2 cut(s) 533, 579
BmsI GCATC 1 cut(s) 925
BoxI GACNNNNGTC 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 1124
BpuMI CCSGG 1 cut(s) 533
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 1206
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 884, 914
Bsc4I CCNNNNNNNGG 1 cut(s) 808
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 1233
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 1206
BseGI GGATG 7 cut(s) 137, 348, 354, 679, 772, 877, 1467
BseLI CCNNNNNNNGG 1 cut(s) 808
BseMI GCAATG 1 cut(s) 1233
BseMII CTCAG 3 cut(s) 574, 1134, 1343
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 3 cut(s) 70, 320, 908
BseXI GCAGC 3 cut(s) 17, 631, 1210
BseYI CCCAGC 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 2 cut(s) 210, 533
BslFI GGGAC 3 cut(s) 160, 705, 812
BslI CCNNNNNNNGG 1 cut(s) 808
BsmAI GTCTC 4 cut(s) 151, 396, 851, 869
BsmBI CGTCTC 2 cut(s) 151, 396
BsmFI GGGAC 3 cut(s) 160, 705, 812
BsnI GGCC 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 123, 590
Bsp143I GATC 3 cut(s) 167, 365, 498
BspACI CCGC 4 cut(s) 193, 567, 809, 890
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 3 cut(s) 573, 1135, 1342
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 5 cut(s) 293, 423, 477, 800, 930
BspMI ACCTGC 2 cut(s) 599, 629
BspPI GGATC 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 1316
BsrDI GCAATG 1 cut(s) 1233
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 1206
BssMI GATC 3 cut(s) 167, 365, 498
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 1206
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 3 cut(s) 99, 1076, 1156
Bst6I CTCTTC 2 cut(s) 993, 1155
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 1316
BstC8I GCNNGC 2 cut(s) 508, 1221
BstDEI CTNAG 5 cut(s) 560, 685, 1124, 1143, 1329
BstF5I GGATG 7 cut(s) 137, 348, 354, 679, 772, 877, 1467
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 3 cut(s) 170, 368, 501
BstMAI GTCTC 4 cut(s) 151, 396, 851, 869
BstMBI GATC 3 cut(s) 167, 365, 498
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 579
BstPAI GACNNNNGTC 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 531, 577
BstSFI CTRYAG 1 cut(s) 1155
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 17, 631, 1210
BsuI GTATCC 1 cut(s) 1438
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 7 cut(s) 137, 348, 354, 679, 772, 877, 1467
BtsIMutI CAGTG 1 cut(s) 293
BveI ACCTGC 2 cut(s) 599, 629
Cac8I GCNNGC 2 cut(s) 508, 1221
CaiI CAGNNNCTG 1 cut(s) 822
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 291, 475, 799
CseI GACGC 2 cut(s) 413, 611
Csp6I GTAC 2 cut(s) 1038, 1072
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
CviAII CATG 1 cut(s) 1408
CviQI GTAC 2 cut(s) 1038, 1072
DdeI CTNAG 5 cut(s) 560, 685, 1124, 1143, 1329
DpnI GATC 3 cut(s) 169, 367, 500
DpnII GATC 3 cut(s) 167, 365, 498
DraI TTTAAA 1 cut(s) 1351
Eam1104I CTCTTC 2 cut(s) 993, 1155
EarI CTCTTC 2 cut(s) 993, 1155
EciI GGCGGA 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 1206
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 291, 799
Eco57I CTGAAG 3 cut(s) 799, 1017, 1307
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 1 cut(s) 577
EcoT14I CCWWGG 1 cut(s) 1206
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 1206
Esp3I CGTCTC 2 cut(s) 151, 396
FaeI CATG 1 cut(s) 1411
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 3 cut(s) 160, 705, 812
FatI CATG 1 cut(s) 1407
FauI CCCGC 1 cut(s) 560
FbaI TGATCA 1 cut(s) 498
Fnu4HI GCNGC 3 cut(s) 6, 620, 1224
FokI GGATG 7 cut(s) 124, 335, 341, 686, 779, 884, 1474
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 3 cut(s) 6, 620, 1224
FspBI CTAG 2 cut(s) 717, 1106
GlaI GCGC 1 cut(s) 319
GluI GCNGC 3 cut(s) 6, 620, 1224
GsaI CCCAGC 1 cut(s) 163
HaeIII GGCC 1 cut(s) 476
HapII CCGG 2 cut(s) 210, 533
HgaI GACGC 2 cut(s) 413, 611
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 1 cut(s) 1411
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HindIII AAGCTT 3 cut(s) 384, 504, 1319
HinfI GANTC 3 cut(s) 515, 604, 1094
HpaII CCGG 2 cut(s) 210, 533
HphI GGTGA 3 cut(s) 87, 208, 796
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 6 cut(s) 103, 118, 167, 498, 1332, 1363
Hpy188III TCNNGA 3 cut(s) 451, 764, 827
Hpy8I GTNNAC 1 cut(s) 291
Hpy99I CGWCG 2 cut(s) 107, 146
HpyAV CCTTC 5 cut(s) 216, 284, 745, 781, 1394
HpyCH4III ACNGT 3 cut(s) 99, 1076, 1156
HpyCH4V TGCA 8 cut(s) 594, 619, 638, 698, 730, 972, 1226, 1376
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 5 cut(s) 560, 685, 1124, 1143, 1329
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 1 cut(s) 1411
HspAI GCGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 3 cut(s) 167, 365, 498
LmnI GCTCC 4 cut(s) 168, 421, 928, 1450
Lsp1109I GCAGC 3 cut(s) 17, 631, 1210
LweI GCATC 1 cut(s) 925
MaeI CTAG 2 cut(s) 717, 1106
MalI GATC 3 cut(s) 169, 367, 500
MboI GATC 3 cut(s) 167, 365, 498
MboII GAAGA 9 cut(s) 10, 58, 81, 274, 532, 917, 1010, 1172, 1319
MhlI GDGCHC 2 cut(s) 123, 590
MluCI AATT 9 cut(s) 9, 490, 525, 743, 1080, 1089, 1132, 1357, 1421
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 5 cut(s) 126, 408, 958, 1284, 1422
MroXI GAANNNNTTC 1 cut(s) 1133
MseI TTAA 4 cut(s) 489, 1151, 1317, 1350
MspCI CTTAAG 1 cut(s) 1316
MspI CCGG 2 cut(s) 210, 533
MspR9I CCNGG 2 cut(s) 533, 579
MssI GTTTAAAC 1 cut(s) 1351
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 1 cut(s) 192
NciI CCSGG 1 cut(s) 533
NdeII GATC 3 cut(s) 167, 365, 498
NlaIII CATG 1 cut(s) 1411
NlaIV GGNNCC 5 cut(s) 293, 423, 477, 800, 930
PaqCI CACCTGC 1 cut(s) 599
PdmI GAANNNNTTC 1 cut(s) 1133
PfeI GAWTC 2 cut(s) 604, 1094
PkrI GCNGC 3 cut(s) 7, 621, 1225
PleI GAGTC 1 cut(s) 523
PmeI GTTTAAAC 1 cut(s) 1351
PpsI GAGTC 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 108
PsiI TTATAA 1 cut(s) 948
Psp6I CCWGG 1 cut(s) 577
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 1 cut(s) 577
PspN4I GGNNCC 5 cut(s) 293, 423, 477, 800, 930
PspPI GGNCC 3 cut(s) 291, 475, 799
PstNI CAGNNNCTG 1 cut(s) 822
RsaI GTAC 2 cut(s) 1039, 1073
RsaNI GTAC 2 cut(s) 1038, 1072
SaqAI TTAA 4 cut(s) 489, 1151, 1317, 1350
SatI GCNGC 3 cut(s) 6, 620, 1224
Sau3AI GATC 3 cut(s) 167, 365, 498
Sau96I GGNCC 3 cut(s) 291, 475, 799
ScaI AGTACT 1 cut(s) 1039
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 533, 579
SduI GDGCHC 2 cut(s) 123, 590
SfaNI GCATC 1 cut(s) 925
SfcI CTRYAG 1 cut(s) 1155
SinI GGWCC 2 cut(s) 291, 799
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
SpeI ACTAGT 2 cut(s) 716, 1105
Sse9I AATT 9 cut(s) 9, 490, 525, 743, 1080, 1089, 1132, 1357, 1421
SsiI CCGC 4 cut(s) 193, 567, 809, 890
SspMI CTAG 2 cut(s) 717, 1106
StyD4I CCNGG 2 cut(s) 531, 577
StyI CCWWGG 1 cut(s) 1206
TaaI ACNGT 3 cut(s) 99, 1076, 1156
TaqI TCGA 1 cut(s) 518
TasI AATT 9 cut(s) 9, 490, 525, 743, 1080, 1089, 1132, 1357, 1421
TatI WGTACW 1 cut(s) 1037
TfiI GAWTC 2 cut(s) 604, 1094
Tru1I TTAA 4 cut(s) 489, 1151, 1317, 1350
Tru9I TTAA 4 cut(s) 489, 1151, 1317, 1350
TscAI CASTG 1 cut(s) 293
TseI GCWGC 3 cut(s) 5, 619, 1223
TspDTI ATGAA 3 cut(s) 756, 1409, 1449
TspGWI ACGGA 2 cut(s) 130, 1463
TspRI CASTG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 1316
VpaK11BI GGWCC 2 cut(s) 291, 799
XapI RAATTY 2 cut(s) 1132, 1357
XmnI GAANNNNTTC 1 cut(s) 1133
XspI CTAG 2 cut(s) 717, 1106
ZrmI AGTACT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.