RchiOBHm_Chr6g0245191

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015238
Physical Location & Seq
Forward (+)
1114407 .. 1117087
2681 bp
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UTR
Exon/CDS
Intron
PRQ21975

Sequence Viewer

Length: 1449 bp
ATGGCGGCAGTTCAAATCTTCAAAAGCTCTCGTCTTTTCCCAGCTACAAAACCCAACCCCCTCCTCTGCCTCCCCCACTTCTCCTCCGCGCCGTCCGATCAAACCCCACCAGAACCCAACCCCCTAATCCCCTCCGTCGTCTCCATCCTAACCAACCTCCGATCCAAGTCCCGCTGGGGCTACCTCCGCTCTCTCTACCCTTCCGGCTTCACCCCAACCGACTTTTCCCAAATCGCCCTCCAAATCAAAAACAATCCCCACCTCGTCCTTCGCTTCTTCCAATGGACCCAAAACCACAACAACCCTCTCTGCTCCCACAACCTCCTCTCCTTCTCCACCACCATCCACATCCTCGCTCGCTCTCGCCTCAAATCCCAAGCCTACTCCCTCATCCGCGACGCCATTCGGGTTTCGGAGCCTCTGCAAGTGTTCGAAGCCCTGGTCAAGACTTACCGGCAATGCGGGTCGGCGCCGTTCGTGTTTGATTACCTGATCAGAGCTTGTTTGGAGTCCAAGAAAATCGACCCGGCATTGCAGATTGTTAGAATGTTGCTCAGTCGCGGCATCAATCCTGGTTTGAACACCTGCAATAGTCTGATTCGGTGCGTAATGCAGGCTCGAGGTGCGTGGGCGGGTTATGAGATTTACAGAGAGGTTTTCGGATTGGATGGTGGGGTTAGGGTTGCGAGAGCTAGGCCGAATGTGCAGACTTTCAATGAGTTGATGTTGGGGTTTTATCAGGACGGGGCGGTGGAGATGGTGGAGGAGATTTGGGACCAAATGCCGGAGTTTGGTTGCTGTGCTGATGTTTATAGTTACAGCATTTTGATGGAGACTTATTGTGAGGACGAGAAAATGAGTAATGCGGAGGAGTTGTGGGAAGAAATGAGAGGTAAGGGTGTGGAGCCTGATGCTGTTGCTTACAACACTATGATTGGGGGGTTTTGCAGAGTTGGGGAGATTGAAATGGCTGAAGAGTTTTTCAAACAAATGGGATTGAGTGGGATAGAGAGTAGTGGTGCCGCTTGCGAGCATCTGGTTAGAGGGTACTGTAAAATAGGGAAAATTGATGAAGCAATGCTAGTGTATAAGGATATGCTTAGGAAAGATTTTAGGGCCGAGAGTTTAACTGTGGATGAGTTGATTAGAGGGCTTTGTGGTGAGAGCAGGGTTTTAGAAGCCTTGGAGGTTATGAGAGCGGCAATGATAGATTATGGTTTTTGTCCGAGGGAGAAGTCGTATGAGTTTTTGATAGGAGGGTTGTGTGAGCAGGGGAAGTTGGAAGAAGCGCTTAAGCTTCAGGCTCAGATGGTTGGTAAAGGGTTTAAACCAAATTCTGAAATCTATAGTGCATTTATTAGTGGATATATGAAAGAAGGGAACAAGGAAGTAGCAGAATTGTTGAGGAATGAAATGTTGGATACTCAGGTGTGTGGGATTGCGGATTAG

Protein Analysis

482

Amino Acids

54.27

Weight (kDa)

5.55

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 233 - 281 6.9e-10 PPR repeat family
PPR_1 PF12854 265 - 297 1.5e-07 PPR repeat
PPR_2 PF13041 276 - 316 5e-11 PPR repeat family
PPR_1 PF12854 299 - 331 2.7e-11 PPR repeat
PPR_2 PF13041 303 - 337 6.4e-09 PPR repeat family
PPR PF01535 306 - 336 5.4e-08 PPR repeat
PPR_2 PF13041 343 - 386 8.6e-07 PPR repeat family
PPR_2 PF13041 413 - 446 1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15980
fragaria_vesca FvH4_2g02001 FvH4_2g02001 FvH4_2g02001
malus_domestica MD10G1084800.v1.1
prunus_persica Prupe.8G107700_v2.0.a1
pyrus_communis pycom10g06650
rosa_chinensis RchiOBHm_Chr6g0245191
rosa_laevigata RLG00000015317
rosa_multiflora Rmu_sc0027529.1_g000005 Rmu_sc0038123.1_g000001
rosa_roxburghii Rroxscaffold_7G00215370
rosa_rugosa Rorug05G0512900
rosa_samantha Rh6AG027800 Rh6BG023900
rosa_wichuraiana Rw6G002350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 469, 1019
AccBSI CCGCTC 2 cut(s) 189, 1199
AccII CGCG 3 cut(s) 89, 396, 561
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 1 cut(s) 1333
AcuI CTGAAG 2 cut(s) 993, 1283
AcyI GRCGYC 2 cut(s) 399, 470
AfaI GTAC 1 cut(s) 1049
AfeI AGCGCT 1 cut(s) 1290
AfiI CCNNNNNNNGG 2 cut(s) 784, 791
AflII CTTAAG 1 cut(s) 1292
AgsI TTSAA 6 cut(s) 14, 22, 580, 715, 965, 985
AjnI CCWGG 2 cut(s) 438, 571
AjuI GAANNNNNNNTTGG 4 cut(s) 208, 240, 1400, 1432
AluBI AGCT 5 cut(s) 27, 44, 500, 692, 1297
AluI AGCT 5 cut(s) 27, 44, 500, 692, 1297
Alw26I GTCTC 2 cut(s) 145, 827
AlwI GGATC 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 618
Aor51HI AGCGCT 1 cut(s) 1290
AoxI GGCC 2 cut(s) 695, 1116
ApoI RAATTY 1 cut(s) 1333
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AspLEI GCGC 3 cut(s) 91, 472, 1291
AspS9I GGNCC 3 cut(s) 285, 775, 1116
AsuC2I CCSGG 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 202, 1172
AsuII TTCGAA 1 cut(s) 432
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 2 cut(s) 285, 775
BaeI ACNNNNGTAYC 2 cut(s) 1413, 1446
BanI GGYRCC 2 cut(s) 469, 1019
BccI CCATC 6 cut(s) 152, 350, 662, 751, 823, 1303
BceAI ACGGC 2 cut(s) 76, 457
BciT130I CCWGG 2 cut(s) 440, 573
BciVI GTATCC 1 cut(s) 1414
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 2 cut(s) 145, 827
BfaI CTAG 2 cut(s) 693, 1082
BfmI CTRYAG 1 cut(s) 1345
BfoI RGCGCY 2 cut(s) 473, 1292
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 1 cut(s) 593
BfuI GTATCC 1 cut(s) 1414
BisI GCNGC 4 cut(s) 6, 562, 1023, 1200
BlsI GCNGC 4 cut(s) 7, 563, 1024, 1201
Bme1390I CCNGG 3 cut(s) 440, 527, 573
Bme18I GGWCC 2 cut(s) 285, 775
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 3 cut(s) 285, 775, 1116
BmiI GGNNCC 6 cut(s) 287, 417, 471, 776, 906, 1021
BmrFI CCNGG 3 cut(s) 440, 527, 573
BmsI GCATC 3 cut(s) 573, 901, 1042
Bpu10I CCTNAGC 1 cut(s) 1100
Bpu14I TTCGAA 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 527
BsaHI GRCGYC 2 cut(s) 399, 470
BsaJI CCNNGG 3 cut(s) 438, 1182, 1226
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 311, 341, 860, 890, 1223, 1253
Bsc4I CCNNNNNNNGG 2 cut(s) 784, 791
Bse118I RCCGGY 1 cut(s) 453
Bse3DI GCAATG 4 cut(s) 464, 530, 1083, 1209
BseBI CCWGG 2 cut(s) 440, 573
BseDI CCNNGG 3 cut(s) 438, 1182, 1226
BseGI GGATG 6 cut(s) 144, 342, 348, 390, 673, 1141
BseLI CCNNNNNNNGG 2 cut(s) 784, 791
BseMI GCAATG 4 cut(s) 464, 530, 1083, 1209
BseMII CTCAG 3 cut(s) 568, 1319, 1439
BseRI GAGGAG 5 cut(s) 53, 73, 314, 779, 884
BseYI CCCAGC 2 cut(s) 40, 174
BsgI GTGCAG 1 cut(s) 725
Bsh1236I CGCG 3 cut(s) 89, 396, 561
BshFI GGCC 2 cut(s) 697, 1118
BshNI GGYRCC 2 cut(s) 469, 1019
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 4 cut(s) 204, 454, 527, 785
BslFI GGGAC 2 cut(s) 154, 788
BslI CCNNNNNNNGG 2 cut(s) 784, 791
BsmAI GTCTC 2 cut(s) 145, 827
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 154, 788
BsnI GGCC 2 cut(s) 697, 1118
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 3 cut(s) 97, 161, 492
BspANI GGCC 2 cut(s) 697, 1118
BspCNI CTCAG 3 cut(s) 567, 1318, 1438
BspFNI CGCG 3 cut(s) 89, 396, 561
BspLI GGNNCC 6 cut(s) 287, 417, 471, 776, 906, 1021
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 1 cut(s) 156
BspT104I TTCGAA 1 cut(s) 432
BspT107I GGYRCC 2 cut(s) 469, 1019
BspTI CTTAAG 1 cut(s) 1292
BsrBI CCGCTC 2 cut(s) 189, 1199
BsrDI GCAATG 4 cut(s) 464, 530, 1083, 1209
BsrFI RCCGGY 1 cut(s) 453
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 3 cut(s) 438, 1182, 1226
BssMI GATC 3 cut(s) 97, 161, 492
BssNI GRCGYC 2 cut(s) 399, 470
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 2 cut(s) 440, 573
Bst4CI ACNGT 2 cut(s) 1052, 1132
Bst6I CTCTTC 1 cut(s) 969
BstACI GRCGYC 2 cut(s) 399, 470
BstAFI CTTAAG 1 cut(s) 1292
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 4 cut(s) 358, 615, 1027, 1031
BstDEI CTNAG 4 cut(s) 554, 1100, 1305, 1425
BstF5I GGATG 6 cut(s) 144, 342, 348, 390, 673, 1141
BstFNI CGCG 3 cut(s) 89, 396, 561
BstH2I RGCGCY 2 cut(s) 473, 1292
BstHHI GCGC 3 cut(s) 91, 472, 1291
BstKTI GATC 3 cut(s) 100, 164, 495
BstMAI GTCTC 2 cut(s) 145, 827
BstMBI GATC 3 cut(s) 97, 161, 492
BstMWI GCNNNNNNNGC 3 cut(s) 186, 623, 703
BstNI CCWGG 2 cut(s) 440, 573
BstSCI CCNGG 3 cut(s) 438, 525, 571
BstSFI CTRYAG 1 cut(s) 1345
BstUI CGCG 3 cut(s) 89, 396, 561
BsuI GTATCC 1 cut(s) 1414
BsuRI GGCC 2 cut(s) 697, 1118
BtsCI GGATG 6 cut(s) 144, 342, 348, 390, 673, 1141
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 4 cut(s) 358, 615, 1027, 1031
CfoI GCGC 3 cut(s) 91, 472, 1291
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 3 cut(s) 285, 775, 1116
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 1048
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 4 cut(s) 554, 1100, 1305, 1425
DinI GGCGCC 1 cut(s) 471
DpnI GATC 3 cut(s) 99, 163, 494
DpnII GATC 3 cut(s) 97, 161, 492
DraI TTTAAA 1 cut(s) 1327
Eam1104I CTCTTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 969
Eco130I CCWWGG 1 cut(s) 1182
Eco47I GGWCC 2 cut(s) 285, 775
Eco47III AGCGCT 1 cut(s) 1290
Eco57I CTGAAG 2 cut(s) 993, 1283
Eco88I CYCGRG 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 438, 571
EcoT14I CCWWGG 1 cut(s) 1182
EgeI GGCGCC 1 cut(s) 471
EheI GGCGCC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 1182
Esp3I CGTCTC 1 cut(s) 145
FalI AAGNNNNNCTT 2 cut(s) 1275, 1307
FaqI GGGAC 2 cut(s) 154, 788
FauI CCCGC 3 cut(s) 179, 455, 625
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 6, 562, 1023, 1200
FokI GGATG 6 cut(s) 131, 329, 335, 377, 680, 1148
Fsp4HI GCNGC 4 cut(s) 6, 562, 1023, 1200
FspBI CTAG 2 cut(s) 693, 1082
GlaI GCGC 3 cut(s) 90, 471, 1290
GluI GCNGC 4 cut(s) 6, 562, 1023, 1200
GsaI CCCAGC 2 cut(s) 44, 178
HaeII RGCGCY 2 cut(s) 473, 1292
HaeIII GGCC 2 cut(s) 697, 1118
HapII CCGG 4 cut(s) 204, 454, 527, 785
HgaI GACGC 1 cut(s) 407
HhaI GCGC 3 cut(s) 91, 472, 1291
Hin1I GRCGYC 2 cut(s) 399, 470
Hin6I GCGC 3 cut(s) 89, 470, 1289
HinP1I GCGC 3 cut(s) 89, 470, 1289
HindIII AAGCTT 1 cut(s) 1295
HinfI GANTC 2 cut(s) 509, 598
HpaII CCGG 4 cut(s) 204, 454, 527, 785
HphI GGTGA 2 cut(s) 202, 1172
Hpy188I TCNGA 9 cut(s) 97, 161, 415, 497, 597, 662, 1227, 1308, 1339
Hpy188III TCNNGA 2 cut(s) 445, 740
Hpy99I CGWCG 2 cut(s) 140, 401
HpyAV CCTTC 4 cut(s) 210, 278, 340, 1370
HpyCH4III ACNGT 2 cut(s) 1052, 1132
HpyCH4V TGCA 7 cut(s) 424, 535, 588, 613, 706, 948, 1352
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 623, 703
HpyF3I CTNAG 4 cut(s) 554, 1100, 1305, 1425
Hsp92I GRCGYC 2 cut(s) 399, 470
HspAI GCGC 3 cut(s) 89, 470, 1289
KasI GGCGCC 1 cut(s) 469
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 3 cut(s) 97, 161, 492
LmnI GCTCC 3 cut(s) 317, 415, 904
LweI GCATC 3 cut(s) 573, 901, 1042
MaeI CTAG 2 cut(s) 693, 1082
MaeIII GTNAC 1 cut(s) 815
MalI GATC 3 cut(s) 99, 163, 494
MbiI CCGCTC 2 cut(s) 189, 1199
MboI GATC 3 cut(s) 97, 161, 492
MboII GAAGA 5 cut(s) 10, 268, 893, 986, 1295
MluCI AATT 3 cut(s) 1065, 1333, 1397
Mly113I GGCGCC 1 cut(s) 470
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 2 cut(s) 1260, 1398
MseI TTAA 3 cut(s) 1127, 1293, 1326
MslI CAYNNNNRTG 1 cut(s) 827
MspA1I CMGCKG 1 cut(s) 174
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 4 cut(s) 204, 454, 527, 785
MspR9I CCNGG 3 cut(s) 440, 527, 573
MssI GTTTAAAC 1 cut(s) 1327
MvaI CCWGG 2 cut(s) 440, 573
MvnI CGCG 3 cut(s) 89, 396, 561
MwoI GCNNNNNNNGC 3 cut(s) 186, 623, 703
NarI GGCGCC 1 cut(s) 470
NciI CCSGG 1 cut(s) 527
NdeII GATC 3 cut(s) 97, 161, 492
NlaIV GGNNCC 6 cut(s) 287, 417, 471, 776, 906, 1021
NmeAIII GCCGAG 1 cut(s) 1144
NspV TTCGAA 1 cut(s) 432
PaeR7I CTCGAG 1 cut(s) 618
PaqCI CACCTGC 1 cut(s) 593
PfeI GAWTC 1 cut(s) 598
PkrI GCNGC 4 cut(s) 7, 563, 1024, 1201
PleI GAGTC 1 cut(s) 517
PluTI GGCGCC 1 cut(s) 473
PmeI GTTTAAAC 1 cut(s) 1327
PpsI GAGTC 1 cut(s) 517
Psp6I CCWGG 2 cut(s) 438, 571
PspFI CCCAGC 2 cut(s) 40, 174
PspGI CCWGG 2 cut(s) 438, 571
PspN4I GGNNCC 6 cut(s) 287, 417, 471, 776, 906, 1021
PspPI GGNCC 3 cut(s) 285, 775, 1116
PspXI VCTCGAGB 1 cut(s) 618
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 1 cut(s) 827
SaqAI TTAA 3 cut(s) 1127, 1293, 1326
SatI GCNGC 4 cut(s) 6, 562, 1023, 1200
Sau3AI GATC 3 cut(s) 97, 161, 492
Sau96I GGNCC 3 cut(s) 285, 775, 1116
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 3 cut(s) 440, 527, 573
SfaNI GCATC 3 cut(s) 573, 901, 1042
SfcI CTRYAG 1 cut(s) 1345
SfoI GGCGCC 1 cut(s) 471
Sfr274I CTCGAG 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 2 cut(s) 285, 775
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 2 cut(s) 618, 1292
SmoI CTYRAG 2 cut(s) 618, 1292
Sse9I AATT 3 cut(s) 1065, 1333, 1397
SspDI GGCGCC 1 cut(s) 469
SspMI CTAG 2 cut(s) 693, 1082
StyD4I CCNGG 3 cut(s) 438, 525, 571
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 2 cut(s) 1052, 1132
TaqI TCGA 3 cut(s) 432, 522, 619
TasI AATT 3 cut(s) 1065, 1333, 1397
TauI GCSGC 4 cut(s) 8, 564, 1025, 1202
TfiI GAWTC 1 cut(s) 598
Tru1I TTAA 3 cut(s) 1127, 1293, 1326
Tru9I TTAA 3 cut(s) 1127, 1293, 1326
TspDTI ATGAA 3 cut(s) 1086, 1385, 1425
TspGWI ACGGA 1 cut(s) 124
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 2 cut(s) 285, 775
XapI RAATTY 1 cut(s) 1333
XhoI CTCGAG 1 cut(s) 618
XspI CTAG 2 cut(s) 693, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.