MD10G1084800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015238
Physical Location & Seq
Forward (+)
12478672 .. 12480138
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1084800.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGCTTTTCAAATCCTCAAACGCACTCTTTCTTCAGCAAGAAAACCCAAACCCCTCTCTGTCTCCCGTTTCTCCTCCTCTTCTCCACCGTCCGATCAAGCTCCATCACAAACCAGCCCCCTAATCTCCGACGTCGTTTCCATCCTAACCCACCAGCGCTCCAAATCCCGATGGAGCTACCTTCACTCTCTCTACCCCAACGGCTTCAACCCGGACGACTTTTCCAAGATTGCCCTCCACATCAAAAACAATCCCCGTCTTGTCCTCGCCTTCTTCCTCTGGACCCAACGCAAGTCCCTCTGCAACCACAACCTCCTCTCTCACTCCACCGTAATCCACATCCTCGCCCGAGCCCGGCTCAGGTCCCAAGCTTACGACCTTATTCGAGCCGCCATTCGGGTATCCGACGAATCTGAGCCTTTGAAAGTGTTTGAGAGTCTTGTCAAGACCTATAGGCAATGTGGGTCGGCGCCCTTTGTGTTTGATTTGCTGGTCAAAGCTTGCTTGGAGTCGAAGAAAATCGACCCATCAATCCAGATTGTGAGAATGTTGCTGTCTCGTGGGATCAGCCCCGGATTGAGCATCTGCAATGCTCTGATTCGGCTTCTTTCGCAGCGTCGAGGTGCGTATGCTGGTTATGAGATTTATAGGGAGATTTTTGGATTGGATTGTGATGTTTTAGATAAAAAGGTGAAAAGGGTTGCTAGAATCAGCCCGAGTGTGCAAACTTTCAATGCATTGATGTTGGGTTTTTATCAGGATGGTGTGGTGGAGAAGGTGAAGGAGATTTGGGATCAAATGGCGGGTTTGAATTGCTGTCCAAATGGCTATAGTTATAGTATTTTGATGGCTGCTTATTGTGAGGAAGATAGAATGAGTGAAGCAGAGGAGTTGTGGGAAGAAATGAGAGCTAAGAGTGTGGTGCCTGATGTTGTTGCTTATAACACTATGATTGGTGGGTTTTGCAAAATCGGAGAAATTGAAATGGCTGAAGAGTTTTTCAAAGAAATGGGATTGAGTGGAGTAGAGAGTACTTGTGCTACGTATGATCATCTTATTATTGGGTACTGTAAAACAGGAAACATTGATTCTGCTATGCTCTTATATAAGGACATGCTTAGGAAAGATTTCAGGCCCGAGTGTTCAACAATGGATGCATTGATTCAGGGGCTTTGTGATGAGAGTAGGGTTTTAGAAGCATTGAATGTTATGAGAGGTGCAACGGTAGATTTTGGTTTTTGTTCGACCGAGAAGAGTTATGAGACTCTGATAAGAGGGTTGTGTGAGGATGAGAAGTTGGAAGAAGCACTTAAGCTTCAGGCCGAGATGGTGGGAAAAGGGTTTAAACCGAATTCTGAGGTCTATCATGCGTTCATTAGTGCGTATATGAAACAAGGGAACAAGGAAGTAGCAGAACGGTTGAAGATAGAAATGTTGGATGCTCAGATGTGTGGGAATGGGGATTAG

Protein Analysis

489

Amino Acids

54.95

Weight (kDa)

7.04

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 239 - 287 5.1e-10 PPR repeat family
PPR_1 PF12854 271 - 303 7.6e-08 PPR repeat
PPR PF01535 277 - 305 2.1e-06 PPR repeat
PPR_2 PF13041 281 - 323 1.2e-12 PPR repeat family
PPR_1 PF12854 306 - 337 6.5e-11 PPR repeat
PPR_2 PF13041 309 - 353 3.2e-12 PPR repeat family
PPR PF01535 312 - 342 2e-08 PPR repeat
PPR_2 PF13041 347 - 393 7.8e-10 PPR repeat family
PPR PF01535 348 - 376 9.1e-06 PPR repeat
PPR_2 PF13041 419 - 454 4.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15980
fragaria_vesca FvH4_2g02001 FvH4_2g02001 FvH4_2g02001
malus_domestica MD10G1084800.v1.1
prunus_persica Prupe.8G107700_v2.0.a1
pyrus_communis pycom10g06650
rosa_chinensis RchiOBHm_Chr6g0245191
rosa_laevigata RLG00000015317
rosa_multiflora Rmu_sc0027529.1_g000005 Rmu_sc0038123.1_g000001
rosa_roxburghii Rroxscaffold_7G00215370
rosa_rugosa Rorug05G0512900
rosa_samantha Rh6AG027800 Rh6BG023900
rosa_wichuraiana Rw6G002350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 942
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 2 cut(s) 469, 922
AciI CCGC 2 cut(s) 390, 803
AclWI GGATC 2 cut(s) 572, 801
AcsI RAATTY 1 cut(s) 1351
AcuI CTGAAG 3 cut(s) 18, 1011, 1301
AcyI GRCGYC 2 cut(s) 132, 470
AfaI GTAC 2 cut(s) 1033, 1067
AfeI AGCGCT 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 354, 360, 396
AflII CTTAAG 1 cut(s) 1310
AluBI AGCT 6 cut(s) 101, 177, 371, 500, 911, 1315
AluI AGCT 6 cut(s) 101, 177, 371, 500, 911, 1315
Alw26I GTCTC 3 cut(s) 67, 561, 1256
AlwI GGATC 2 cut(s) 572, 801
Ama87I CYCGRG 3 cut(s) 348, 715, 1136
Aor51HI AGCGCT 1 cut(s) 158
AoxI GGCC 2 cut(s) 1133, 1320
ApeKI GCWGC 2 cut(s) 613, 851
ApoI RAATTY 1 cut(s) 1351
Asp700I GAANNNNTTC 1 cut(s) 1127
AspLEI GCGC 2 cut(s) 159, 472
AspS9I GGNCC 3 cut(s) 282, 363, 1134
AsuC2I CCSGG 3 cut(s) 212, 355, 573
AsuHPI GGTGA 2 cut(s) 703, 790
AvaI CYCGRG 3 cut(s) 348, 715, 1136
AvaII GGWCC 2 cut(s) 282, 363
BanI GGYRCC 2 cut(s) 469, 922
BanII GRGCYC 1 cut(s) 355
BauI CACGAG 1 cut(s) 558
BbvI GCAGC 2 cut(s) 625, 838
BccI CCATC 7 cut(s) 112, 149, 165, 535, 755, 841, 1321
BceAI ACGGC 1 cut(s) 217
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 1048
BcnI CCSGG 3 cut(s) 212, 355, 573
BcoDI GTCTC 3 cut(s) 67, 561, 1256
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 2 cut(s) 451, 829
BfoI RGCGCY 2 cut(s) 160, 473
BfrI CTTAAG 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 3 cut(s) 390, 614, 852
BlsI GCNGC 3 cut(s) 391, 615, 853
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 3 cut(s) 212, 355, 573
Bme18I GGWCC 2 cut(s) 282, 363
BmeT110I CYCGRG 3 cut(s) 348, 715, 1136
BmgT120I GGNCC 3 cut(s) 282, 363, 1134
BmiI GGNNCC 4 cut(s) 284, 365, 471, 924
BmrFI CCNGG 3 cut(s) 212, 355, 573
BmsI GCATC 3 cut(s) 591, 1144, 1429
BplI GAGNNNNNCTC 2 cut(s) 342, 374
Bpu10I CCTNAGC 2 cut(s) 359, 1118
BpuMI CCSGG 3 cut(s) 212, 355, 573
BsaAI YACGTR 1 cut(s) 1044
BsaHI GRCGYC 2 cut(s) 132, 470
BsaJI CCNNGG 1 cut(s) 571
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 360, 396
Bse3DI GCAATG 2 cut(s) 464, 595
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 6 cut(s) 141, 339, 766, 1159, 1294, 1444
BseLI CCNNNNNNNGG 3 cut(s) 354, 360, 396
BseMI GCAATG 2 cut(s) 464, 595
BseMII CTCAG 4 cut(s) 373, 405, 1347, 1457
BseRI GAGGAG 4 cut(s) 64, 67, 305, 902
BseXI GCAGC 2 cut(s) 625, 838
Bsh1285I CGRYCG 1 cut(s) 1248
BshFI GGCC 2 cut(s) 1135, 1322
BshNI GGYRCC 2 cut(s) 469, 922
BsiEI CGRYCG 1 cut(s) 1248
BsiHKCI CYCGRG 3 cut(s) 348, 715, 1136
BsiSI CCGG 3 cut(s) 212, 355, 573
BslFI GGGAC 2 cut(s) 280, 349
BslI CCNNNNNNNGG 3 cut(s) 354, 360, 396
BsmAI GTCTC 3 cut(s) 67, 561, 1256
BsmFI GGGAC 2 cut(s) 280, 349
BsnI GGCC 2 cut(s) 1135, 1322
BsoBI CYCGRG 3 cut(s) 348, 715, 1136
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 4 cut(s) 94, 564, 793, 1048
BspACI CCGC 2 cut(s) 390, 803
BspANI GGCC 2 cut(s) 1135, 1322
BspCNI CTCAG 4 cut(s) 372, 406, 1348, 1456
BspLI GGNNCC 4 cut(s) 284, 365, 471, 924
BspPI GGATC 2 cut(s) 572, 801
BspT107I GGYRCC 2 cut(s) 469, 922
BspTI CTTAAG 1 cut(s) 1310
BsrDI GCAATG 2 cut(s) 464, 595
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 4 cut(s) 94, 564, 793, 1048
BssNI GRCGYC 2 cut(s) 132, 470
BssSI CACGAG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 558
Bst4CI ACNGT 5 cut(s) 90, 331, 1070, 1225, 1419
Bst6I CTCTTC 3 cut(s) 85, 987, 1247
BstACI GRCGYC 2 cut(s) 132, 470
BstAFI CTTAAG 1 cut(s) 1310
BstBAI YACGTR 1 cut(s) 1044
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 6 cut(s) 359, 414, 912, 1118, 1356, 1443
BstF5I GGATG 6 cut(s) 141, 339, 766, 1159, 1294, 1444
BstH2I RGCGCY 2 cut(s) 160, 473
BstHHI GCGC 2 cut(s) 159, 472
BstKTI GATC 4 cut(s) 97, 567, 796, 1051
BstMAI GTCTC 3 cut(s) 67, 561, 1256
BstMBI GATC 4 cut(s) 94, 564, 793, 1048
BstMCI CGRYCG 1 cut(s) 1248
BstMWI GCNNNNNNNGC 1 cut(s) 610
BstNSI RCATGY 1 cut(s) 1117
BstSCI CCNGG 3 cut(s) 210, 353, 571
BstSFI CTRYAG 2 cut(s) 451, 829
BstSNI TACGTA 1 cut(s) 1044
BstV1I GCAGC 2 cut(s) 625, 838
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 2 cut(s) 1135, 1322
BtsCI GGATG 6 cut(s) 141, 339, 766, 1159, 1294, 1444
Cac8I GCNNGC 1 cut(s) 502
CfoI GCGC 2 cut(s) 159, 472
Cfr13I GGNCC 3 cut(s) 282, 363, 1134
CseI GACGC 1 cut(s) 605
Csp6I GTAC 2 cut(s) 1032, 1066
CviAII CATG 2 cut(s) 1114, 1367
CviQI GTAC 2 cut(s) 1032, 1066
DdeI CTNAG 6 cut(s) 359, 414, 912, 1118, 1356, 1443
DinI GGCGCC 1 cut(s) 471
DpnI GATC 4 cut(s) 96, 566, 795, 1050
DpnII GATC 4 cut(s) 94, 564, 793, 1048
DraI TTTAAA 1 cut(s) 1345
Eam1104I CTCTTC 3 cut(s) 85, 987, 1247
EarI CTCTTC 3 cut(s) 85, 987, 1247
Eco105I TACGTA 1 cut(s) 1044
Eco24I GRGCYC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 282, 363
Eco47III AGCGCT 1 cut(s) 158
Eco57I CTGAAG 3 cut(s) 18, 1011, 1301
Eco88I CYCGRG 3 cut(s) 348, 715, 1136
EcoO109I RGGNCCY 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 1351
EcoT22I ATGCAT 2 cut(s) 739, 1159
EcoT38I GRGCYC 1 cut(s) 355
EgeI GGCGCC 1 cut(s) 471
EheI GGCGCC 1 cut(s) 471
FaeI CATG 2 cut(s) 1117, 1370
FalI AAGNNNNNCTT 2 cut(s) 1293, 1325
FaqI GGGAC 2 cut(s) 280, 349
FatI CATG 2 cut(s) 1113, 1366
FauI CCCGC 1 cut(s) 796
FbaI TGATCA 1 cut(s) 1048
Fnu4HI GCNGC 3 cut(s) 390, 614, 852
FokI GGATG 6 cut(s) 128, 326, 773, 1166, 1301, 1451
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 3 cut(s) 390, 614, 852
FspBI CTAG 1 cut(s) 705
GlaI GCGC 2 cut(s) 158, 471
GluI GCNGC 3 cut(s) 390, 614, 852
HaeII RGCGCY 2 cut(s) 160, 473
HaeIII GGCC 2 cut(s) 1135, 1322
HapII CCGG 3 cut(s) 212, 355, 573
HgaI GACGC 1 cut(s) 605
HhaI GCGC 2 cut(s) 159, 472
Hin1I GRCGYC 2 cut(s) 132, 470
Hin1II CATG 2 cut(s) 1117, 1370
Hin6I GCGC 2 cut(s) 157, 470
HinP1I GCGC 2 cut(s) 157, 470
HindIII AAGCTT 3 cut(s) 369, 498, 1313
HinfI GANTC 8 cut(s) 410, 436, 509, 598, 708, 1088, 1162, 1264
HpaII CCGG 3 cut(s) 212, 355, 573
HphI GGTGA 2 cut(s) 703, 790
Hpy188I TCNGA 9 cut(s) 94, 130, 406, 415, 597, 974, 1269, 1357, 1446
Hpy188III TCNNGA 5 cut(s) 168, 280, 445, 535, 758
Hpy99I CGWCG 4 cut(s) 134, 137, 410, 621
HpyAV CCTTC 4 cut(s) 191, 280, 769, 775
HpyCH4III ACNGT 5 cut(s) 90, 331, 1070, 1225, 1419
HpyCH4IV ACGT 2 cut(s) 132, 1043
HpyCH4V TGCA 7 cut(s) 303, 588, 724, 737, 966, 1157, 1220
HpyF10VI GCNNNNNNNGC 1 cut(s) 610
HpyF3I CTNAG 6 cut(s) 359, 414, 912, 1118, 1356, 1443
HpySE526I ACGT 2 cut(s) 132, 1043
Hsp92I GRCGYC 2 cut(s) 132, 470
Hsp92II CATG 2 cut(s) 1117, 1370
HspAI GCGC 2 cut(s) 157, 470
KasI GGCGCC 1 cut(s) 469
Ksp22I TGATCA 1 cut(s) 1048
Kzo9I GATC 4 cut(s) 94, 564, 793, 1048
LmnI GCTCC 3 cut(s) 106, 164, 174
Lsp1109I GCAGC 2 cut(s) 625, 838
LweI GCATC 3 cut(s) 591, 1144, 1429
MaeI CTAG 1 cut(s) 705
MaeII ACGT 2 cut(s) 132, 1043
MalI GATC 4 cut(s) 96, 566, 795, 1050
MboI GATC 4 cut(s) 94, 564, 793, 1048
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 3 cut(s) 811, 978, 1351
Mly113I GGCGCC 1 cut(s) 470
MlyI GAGTC 3 cut(s) 445, 518, 1258
MmeI TCCRAC 4 cut(s) 153, 429, 1278, 1416
Mph1103I ATGCAT 2 cut(s) 739, 1159
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 2 cut(s) 1311, 1344
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 3 cut(s) 212, 355, 573
MspR9I CCNGG 3 cut(s) 212, 355, 573
MssI GTTTAAAC 1 cut(s) 1345
MwoI GCNNNNNNNGC 1 cut(s) 610
NarI GGCGCC 1 cut(s) 470
NciI CCSGG 3 cut(s) 212, 355, 573
NdeII GATC 4 cut(s) 94, 564, 793, 1048
NlaIII CATG 2 cut(s) 1117, 1370
NlaIV GGNNCC 4 cut(s) 284, 365, 471, 924
NmeAIII GCCGAG 1 cut(s) 1348
NsiI ATGCAT 2 cut(s) 739, 1159
NspI RCATGY 1 cut(s) 1117
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 5 cut(s) 410, 598, 708, 1088, 1162
PkrI GCNGC 3 cut(s) 391, 615, 853
PleI GAGTC 3 cut(s) 444, 517, 1258
PluTI GGCGCC 1 cut(s) 473
PmeI GTTTAAAC 1 cut(s) 1345
PpsI GAGTC 3 cut(s) 444, 517, 1258
Ppu21I YACGTR 1 cut(s) 1044
PpuMI RGGWCCY 1 cut(s) 363
PsiI TTATAA 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 363
PspN4I GGNNCC 4 cut(s) 284, 365, 471, 924
PspPI GGNCC 3 cut(s) 282, 363, 1134
PspPPI RGGWCCY 1 cut(s) 363
RsaI GTAC 2 cut(s) 1033, 1067
RsaNI GTAC 2 cut(s) 1032, 1066
SaqAI TTAA 2 cut(s) 1311, 1344
SatI GCNGC 3 cut(s) 390, 614, 852
Sau3AI GATC 4 cut(s) 94, 564, 793, 1048
Sau96I GGNCC 3 cut(s) 282, 363, 1134
ScaI AGTACT 1 cut(s) 1033
SchI GAGTC 3 cut(s) 445, 518, 1258
ScrFI CCNGG 3 cut(s) 212, 355, 573
SduI GDGCHC 1 cut(s) 355
SfaNI GCATC 3 cut(s) 591, 1144, 1429
SfcI CTRYAG 2 cut(s) 451, 829
SfoI GGCGCC 1 cut(s) 471
SinI GGWCC 2 cut(s) 282, 363
SmlI CTYRAG 1 cut(s) 1310
SmoI CTYRAG 1 cut(s) 1310
SnaBI TACGTA 1 cut(s) 1044
Sse9I AATT 3 cut(s) 811, 978, 1351
SsiI CCGC 2 cut(s) 390, 803
SspDI GGCGCC 1 cut(s) 469
SspMI CTAG 1 cut(s) 705
StyD4I CCNGG 3 cut(s) 210, 353, 571
TaaI ACNGT 5 cut(s) 90, 331, 1070, 1225, 1419
TaiI ACGT 2 cut(s) 135, 1046
TaqI TCGA 5 cut(s) 385, 512, 522, 619, 1244
TaqII GACCGA 1 cut(s) 1262
TasI AATT 3 cut(s) 811, 978, 1351
TatI WGTACW 1 cut(s) 1031
TauI GCSGC 1 cut(s) 392
TfiI GAWTC 5 cut(s) 410, 598, 708, 1088, 1162
Tru1I TTAA 2 cut(s) 1311, 1344
Tru9I TTAA 2 cut(s) 1311, 1344
TseI GCWGC 2 cut(s) 613, 851
TspDTI ATGAA 2 cut(s) 1363, 1403
Vha464I CTTAAG 1 cut(s) 1310
VpaK11BI GGWCC 2 cut(s) 282, 363
XapI RAATTY 1 cut(s) 1351
XceI RCATGY 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 1 cut(s) 705
ZraI GACGTC 1 cut(s) 133
ZrmI AGTACT 1 cut(s) 1033
Zsp2I ATGCAT 2 cut(s) 739, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.