Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002935

Physical Location & Seq

Genomic Coordinates Reverse (-)

69722680 .. 69729895

Gene Structure 7216 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0512900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGCTTCCTAGAAAGAGACCTTGTGAAGGAGAAGAAGGAGACGGTGACGTTAACAAGACCACTGGTACCAGCTCGGCGGCTTCTGCGTCTTTGATCAAGAAGCTTCGGATCGAATCCTACGTTGCCGAATCGGCCATCAACAACAGCAGCAGCAACGGTAGCGGGAGCGTGGTTGAGAACGACGTGCCGATCATGGCTTTGGGAGATTCCAACGCCGGAGATATCGATGAGGATCTGCACAGCCGGCAGCTTGCCGTCTATGGCCGGGAGACGATGAGGAGGCTCTTTGCATCCAATGTTCTGATCTCAGGGGTACAGGGCCTCGGTGCTGAGATTGCCAAAAATCTCATTCTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAGGGACTGTGGAGCTTTGGGATTTGTCCAGCAATTTTATTTTTACAGAGGATGATGTTGGTAAGAACAGGGCACTTGCTTCTGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGATTGTTCATACCTTGACAACTCCATTGACTGCGGAGCAGCTTTCTGATTTCCAGGCTGTTGTATTCACTGATATCAGTTACGAGAAAGCTATTGAATTCAATGATTATTGTCATAATCATCAGCCGCCAATCGCCTTCATAAGAACCGAAGTTAGAGGCCTTTTTGGTTCTGTATTTTGTGACTTTGGACCTGAATTCACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATTATTGCATCAATCAGCAATGACAACCCTGCTATTGTGTCATGTGTCGATGATGAAAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAAGTTCATGGAATGACAGAACTAAATGATGGAAAGCCAAGGAAGATAAAAAATGCTAGGGCTTACTCATTTACTCTTGAGGAGGATACCTCAGGTTTTGGTACCTATGTGAAAGGTGGCATTGTCACACAAGTGAAGCAGCCAAAAGTGTTGAACTTCAAGCCATTGGGGGAAGCACTCAAGAATCCTGGTGATTTTCTTCTTAGTGATTTCTCCAAGTTTGATCGCCCTCCTCTCCTACACTTGGCATTTCAAGCACTGGACAAATTTGTTTCTGAGTTGGGACGATTCCCTGTTGCTGGTTCAGAAGAGGATGCTCAGAAGCTTATATCTGTTGCTACTAACATTAATGATAAACTGGGAGATGGCAGGCTGGAAGATCTTAATCCAAAACTTTTGCGCCACTTCGCCTTTGGTGCAAAGGCAGTACTAAGTCCCATGGCTGCCATGTTTGGTGGTATTGTTGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTTCATCCACTCTTCCAGTTCTTCTACTTTGACTCAGTAGAATCACTTCCAACGGAGCCACTGGATTCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAGATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAACTGGAGGATGCCAAAGTATTCATGGTTGGATCTGGTGCACTTGGATGTGAGTTGTTGAAAAATGTTGCCCTGATGGGAGTTTCATGTGGCGACCAAGGGAAGCTAACAATCACTGATGATGATGTAATTGAGAAGAGTAACCTCAGTAGGCAGTTCCTTTTCCGTGATTGGAACATTGGGCAGGCAAAGTCTACAGTTGCCGCTTCTGCTGCTGCATCAATAAATCCCCGTCTCAACATTGTAGCGTTGCAGAATAGAGTTGGCCCTGAAACTGAGAATGTGTTTGATGATACCTACTGGGAGAATTTAAGTGTTGTTATTAATGCACTAGATAATGTCAATGCCAGGCTCTATGTTGATCAGCGGTGCTTATATTTCCAGAAGGCACTTCTTGAGTCGGGTACTCTGGGTGCGAAATGCAACACTCAGATGGTTATTCCTCACCTAACAGAAAACTATGGTGCCTCTAGAGATCCTCCAGAGAAACAAGCACCCATGTGTACTGTCCACTCATTTCCACACAACATTGATCACTGTCTGACATGGGCTCGATCTGAGTTTGAGGGTTTGCTTGAGAAAACTCCAGCGGAAGTGAATGCATATTTATCCAAGCCAAGTGAATATACTGCTGCAATGAGTAATGCTGGGGATGCACAGGCCAGGGACACCTTGGAGCGTGTTCTTGAGTGCCTTTCCAGAGAAAGATGCGAAACATTCCAAGATTGTATTGCCTGGGCGCGCCTCAAGTTTGAAGATTATTTTTCCGACCGGGTGAAACAGCTGACATATACTTTCCCTGAAGATGCTGCAACTAGTACTGGGGCTCCATTCTGGTCTGCCCCCAAGCGATTCCCCCGTGCACTGCAGTTCTCAGCTAATGATCCCGGTCATCTCCATTTTGTTATGGCAGCATCCATACTCAGGGCAGAGACATTTGGGATCCCAATCCCCGACTGGGTTAGGAACTCTAAGAAGTTGTCTGAAGCTGTTGAGAAAGTGGAAGTCCCAGACTTTCAGCCCAAGAAAGATGCAAAAATAGTGACAGATGATAAGGCCACCAATTTAACTCCTCAATCTATAGATGATGCACAGGTTATTAATGAATTGATTATCAAGTTAGAGCATTGTCGAGAGAAACTCCCACCAGGGTTCCGGATGAAACCGATTCAATTCGAAAAGGATGATGATACCAATTACCATATGGATATGATAGCTGGGCTTGCCAATATGAGGGCTAGGAATTACAGCATTCCTGAGGTGGACAAGCTGAAAGCCAAGTTTATTGCCGGTAGGATTATCCCTGCAATTGCAACCTCCACTGCAATGGCCACAGGACTTGTGTGCCTGGAACTTTACAAGGTTCTGGATGGCGGCCACAAACTGGAAGACTACAGAAATACATTTGCAAACCTAGCACTACCTTTGTTCTCCATGGCTGAGCCGGTACCTCCCAAGGTCATTAAGCACCAGGACATGAGCTGGACAGTGTGGGATAGGTGGATCCTGAGGGGCAATCCTACTCTGAGGGAACTTCTCCAGTGGCTTAAGGATAAGGGACTGAATGCTTATAGCATCTCTTGTGGAAGCTCTCTGCTTTTTAACAGCATGTTTGCTCGACACAAAGACCGAATGGACAAGAAGGTGGTGGATCTGGCTAAGGACGTGGCGAAGGTGGAAATTCCTCCATATCGTTGCCACTTGGATGTTGTTGTTGCTTGCGAGGATGATGAGGACAATGACATTGACATCCCTCTGGTGTCGGTTTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1082

Amino Acids

119.98

Weight (kDa)

5.17

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	80 - 455	1.9e-27	ThiF family
E1_FCCH	PF16190	254 - 324	7.9e-29	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	325 - 395	1.1e-25	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	477 - 969	9.4e-59	ThiF family
UBA_E1_SCCH	PF10585	664 - 917	1.2e-87	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	988 - 1077	2.2e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002935)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75430 AT1G75430
fragaria_vesca	FvH4_2g39270 FvH4_2g39270 FvH4_2g39270
malus_domestica	MD08G1024300.v1.1 MD15G1021900.v1.1
prunus_persica	Prupe.1G373700_v2.0.a1 Prupe.1G373700_v2.0.a1 Prupe.1G373700_v2.0.a1 Prupe.1G373700_v2.0.a1 Prupe.1G373700_v2.0.a1
pyrus_communis	pycom08g01910 pycom15g01820
rosa_chinensis	RchiOBHm_Chr6g0245211 RchiOBHm_Chr6g0301741
rosa_laevigata	RLG00000011226 RLG00000015315 RLG00000021395
rosa_multiflora	Rmu_sc0001288.1_g000006 Rmu_sc0005454.1_g000025
rosa_roxburghii	Rroxscaffold_3G00242160 Rroxscaffold_7G00166460 Rroxscaffold_7G00215350
rosa_rugosa	Rorug05G0512900 Rorug05G0512900 Rorug06G0308600 Rorug06G0308700.1 Rorug06G0308800.1 Rorug06G0308900.1
rosa_samantha	Rh6AG028000 Rh6AG028100 Rh6AG421100 Rh6BG024200 Rh6BG427800 Rh6CG434400 Rh6DG421300
rosa_wichuraiana	Rw6G036450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	3 cut(s)	65, 935, 2917
AccB1I	GGYRCC	4 cut(s)	65, 935, 1934, 2917
AccB7I	CCANNNNNTGG	1 cut(s)	2854
AccI	GTMKAC	1 cut(s)	1664
AccII	CGCG	1 cut(s)	2212
AccIII	TCCGGA	1 cut(s)	2625
AciI	CCGC	8 cut(s)	77, 162, 533, 626, 1674, 1837, 2060, 2844
AcoI	YGGCCR	4 cut(s)	132, 262, 2799, 2845
AcsI	RAATTY	6 cut(s)	596, 695, 803, 1100, 1777, 3150
AcuI	CTGAAG	2 cut(s)	2292, 2475
AfaI	GTAC	8 cut(s)	67, 315, 937, 1263, 1876, 1975, 2290, 2919
AfiI	CCNNNNNNNGG	8 cut(s)	26, 734, 1078, 1133, 2394, 2428, 2703, 2854
AflII	CTTAAG	1 cut(s)	3017
AhlI	ACTAGT	1 cut(s)	2285
AjiI	CACGTC	2 cut(s)	184, 3136
AjnI	CCWGG	8 cut(s)	552, 1021, 1817, 2132, 2204, 2617, 2817, 2940
AleI	CACNNNNGTG	2 cut(s)	965, 1969
Alw21I	GWGCWC	2 cut(s)	1513, 2335
Alw26I	GTCTC	5 cut(s)	10, 33, 263, 1709, 2396
Alw44I	GTGCAC	2 cut(s)	1509, 2331
Aor13HI	TCCGGA	1 cut(s)	2625
AoxI	GGCC	9 cut(s)	132, 262, 319, 658, 1735, 2130, 2526, 2799, 2845
ApaLI	GTGCAC	2 cut(s)	1509, 2331
ApoI	RAATTY	6 cut(s)	596, 695, 803, 1100, 1777, 3150
AscI	GGCGCGCC	1 cut(s)	2210
AseI	ATTAAT	3 cut(s)	1182, 1794, 2571
Asp700I	GAANNNNTTC	1 cut(s)	1377
Asp718I	GGTACC	3 cut(s)	65, 935, 2917
AspLEI	GCGC	3 cut(s)	1236, 2212, 2214
AspS9I	GGNCC	4 cut(s)	319, 689, 1455, 1736
AsuC2I	CCSGG	3 cut(s)	266, 2243, 2358
AsuHPI	GGTGA	4 cut(s)	56, 1037, 1907, 2257
AsuII	TTCGAA	1 cut(s)	2646
AvaII	GGWCC	2 cut(s)	689, 1455
AxyI	CCTNAGG	3 cut(s)	925, 2727, 2978
BaeGI	GKGCMC	3 cut(s)	460, 1513, 2335
BaeI	ACNNNNGTAYC	2 cut(s)	927, 960
BalI	TGGCCA	1 cut(s)	2801
BamHI	GGATCC	2 cut(s)	2412, 2973
BanI	GGYRCC	4 cut(s)	65, 935, 1934, 2917
BanII	GRGCYC	2 cut(s)	2023, 2299
BarI	GAAGNNNNNNTAC	2 cut(s)	1362, 1394
BbsI	GAAGAC	1 cut(s)	2865
Bbv12I	GWGCWC	2 cut(s)	1513, 2335
BccI	CCATC	8 cut(s)	143, 710, 806, 857, 1193, 1540, 1897, 2834
BceAI	ACGGC	2 cut(s)	239, 1410
BciT130I	CCWGG	8 cut(s)	554, 1023, 1819, 2134, 2206, 2619, 2819, 2942
BciVI	GTATCC	1 cut(s)	913
BclI	TGATCA	3 cut(s)	93, 1831, 2002
BcnI	CCSGG	3 cut(s)	266, 2243, 2358
BcoDI	GTCTC	5 cut(s)	10, 33, 263, 1709, 2396
BcuI	ACTAGT	1 cut(s)	2285
BfaI	CTAG	7 cut(s)	9, 891, 1802, 1941, 2286, 2709, 2885
BfmI	CTRYAG	5 cut(s)	1463, 1665, 2336, 2550, 2863
BfrI	CTTAAG	1 cut(s)	3017
BfuI	GTATCC	1 cut(s)	913
BglI	GCCNNNNNGGC	1 cut(s)	131
BglII	AGATCT	1 cut(s)	1213
BlpI	GCTNAGC	1 cut(s)	2910
BmcAI	AGTACT	2 cut(s)	1263, 2290
Bme18I	GGWCC	2 cut(s)	689, 1455
BmgBI	CACGTC	2 cut(s)	184, 3136
BmgT120I	GGNCC	4 cut(s)	319, 689, 1455, 1736
BmrI	ACTGGG	4 cut(s)	1202, 1780, 2301, 2437
BmuI	ACTGGG	4 cut(s)	1202, 1780, 2301, 2437
BpiI	GAAGAC	1 cut(s)	2865
BplI	GAGNNNNNCTC	4 cut(s)	908, 940, 2595, 2627
BpmI	CTGGAG	4 cut(s)	1496, 1935, 2040, 2993
Bpu10I	CCTNAGC	1 cut(s)	3129
Bpu1102I	GCTNAGC	1 cut(s)	2910
Bpu14I	TTCGAA	1 cut(s)	2646
BpuEI	CTTGAG	6 cut(s)	932, 998, 1886, 2066, 2177, 2201
BpuMI	CCSGG	3 cut(s)	266, 2243, 2358
Bsa29I	ATCGAT	1 cut(s)	225
BsaBI	GATNNNNATC	4 cut(s)	231, 816, 1218, 2417
BsaI	GGTCTC	1 cut(s)	10
BsaWI	WCCGGW	1 cut(s)	2625
BsaXI	ACNNNNNCTCC	2 cut(s)	2281, 2311
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 734, 1078, 1133, 2394, 2428, 2703, 2854
Bse118I	RCCGGY	3 cut(s)	243, 2759, 2914
Bse21I	CCTNAGG	3 cut(s)	925, 2727, 2978
Bse3DI	GCAATG	3 cut(s)	763, 2112, 2802
Bse8I	GATNNNNATC	4 cut(s)	231, 816, 1218, 2417
BseAI	TCCGGA	1 cut(s)	2625
BseBI	CCWGG	8 cut(s)	554, 1023, 1819, 2134, 2206, 2619, 2819, 2942
BseCI	ATCGAT	1 cut(s)	225
BseJI	GATNNNNATC	4 cut(s)	231, 816, 1218, 2417
BseLI	CCNNNNNNNGG	8 cut(s)	26, 734, 1078, 1133, 2394, 2428, 2703, 2854
BseMI	GCAATG	3 cut(s)	763, 2112, 2802
BsePI	GCGCGC	1 cut(s)	2210
BseRI	GAGGAG	4 cut(s)	292, 929, 1056, 2532
BseSI	GKGCMC	3 cut(s)	460, 1513, 2335
BseYI	CCCAGC	2 cut(s)	2117, 2687
BsgI	GTGCAG	1 cut(s)	221
Bsh1236I	CGCG	1 cut(s)	2212
Bsh1285I	CGRYCG	1 cut(s)	2242
BshFI	GGCC	9 cut(s)	134, 264, 321, 660, 1737, 2132, 2528, 2801, 2847
BshNI	GGYRCC	4 cut(s)	65, 935, 1934, 2917
BshVI	ATCGAT	1 cut(s)	225
BsiEI	CGRYCG	1 cut(s)	2242
BsiHKAI	GWGCWC	2 cut(s)	1513, 2335
BsiSI	CCGG	8 cut(s)	216, 244, 265, 2242, 2358, 2626, 2760, 2915
BslFI	GGGAC	6 cut(s)	403, 1131, 1254, 2150, 2462, 3042
BslI	CCNNNNNNNGG	8 cut(s)	26, 734, 1078, 1133, 2394, 2428, 2703, 2854
BsmAI	GTCTC	5 cut(s)	10, 33, 263, 1709, 2396
BsmBI	CGTCTC	3 cut(s)	33, 263, 1709
BsmFI	GGGAC	6 cut(s)	403, 1131, 1254, 2150, 2462, 3042
BsmI	GAATGC	3 cut(s)	2074, 2721, 3040
BsnI	GGCC	9 cut(s)	134, 264, 321, 660, 1737, 2132, 2528, 2801, 2847
Bso31I	GGTCTC	1 cut(s)	10
Bsp119I	TTCGAA	1 cut(s)	2646
Bsp1286I	GDGCHC	5 cut(s)	460, 1513, 2023, 2299, 2335
Bsp13I	TCCGGA	1 cut(s)	2625
Bsp1720I	GCTNAGC	1 cut(s)	2910
Bsp19I	CCATGG	2 cut(s)	1272, 2904
BspACI	CCGC	8 cut(s)	77, 162, 533, 626, 1674, 1837, 2060, 2844
BspANI	GGCC	9 cut(s)	134, 264, 321, 660, 1737, 2132, 2528, 2801, 2847
BspDI	ATCGAT	1 cut(s)	225
BspEI	TCCGGA	1 cut(s)	2625
BspFNI	CGCG	1 cut(s)	2212
BspMAI	CTGCAG	1 cut(s)	2340
BspT104I	TTCGAA	1 cut(s)	2646
BspT107I	GGYRCC	4 cut(s)	65, 935, 1934, 2917
BspTI	CTTAAG	1 cut(s)	3017
BspTNI	GGTCTC	1 cut(s)	10
BsrDI	GCAATG	3 cut(s)	763, 2112, 2802
BsrFI	RCCGGY	3 cut(s)	243, 2759, 2914
BssAI	RCCGGY	3 cut(s)	243, 2759, 2914
BssHII	GCGCGC	1 cut(s)	2210
BssT1I	CCWWGG	6 cut(s)	872, 1272, 1567, 2142, 2904, 2925
Bst2UI	CCWGG	8 cut(s)	554, 1023, 1819, 2134, 2206, 2619, 2819, 2942
Bst4CI	ACNGT	8 cut(s)	44, 158, 394, 703, 1669, 1978, 2009, 2959
Bst6I	CTCTTC	3 cut(s)	1137, 1349, 1601
BstAFI	CTTAAG	1 cut(s)	3017
BstBI	TTCGAA	1 cut(s)	2646
BstC8I	GCNNGC	8 cut(s)	245, 252, 1205, 1321, 1656, 2212, 2694, 3190
BstDSI	CCRYGG	2 cut(s)	1272, 2904
BstENI	CCTNNNNNAGG	1 cut(s)	24
BstFNI	CGCG	1 cut(s)	2212
BstHHI	GCGC	3 cut(s)	1236, 2212, 2214
BstMAI	GTCTC	5 cut(s)	10, 33, 263, 1709, 2396
BstMCI	CGRYCG	1 cut(s)	2242
BstNI	CCWGG	8 cut(s)	554, 1023, 1819, 2134, 2206, 2619, 2819, 2942
BstNSI	RCATGY	1 cut(s)	3082
BstSFI	CTRYAG	5 cut(s)	1463, 1665, 2336, 2550, 2863
BstSLI	GKGCMC	3 cut(s)	460, 1513, 2335
BstUI	CGCG	1 cut(s)	2212
BstV2I	GAAGAC	1 cut(s)	2865
BstX2I	RGATCY	8 cut(s)	232, 817, 1213, 1502, 1945, 2412, 2973, 3121
BstXI	CCANNNNNNTGG	1 cut(s)	2797
BstYI	RGATCY	8 cut(s)	232, 817, 1213, 1502, 1945, 2412, 2973, 3121
Bsu15I	ATCGAT	1 cut(s)	225
Bsu36I	CCTNAGG	3 cut(s)	925, 2727, 2978
BsuI	GTATCC	1 cut(s)	913
BsuRI	GGCC	9 cut(s)	134, 264, 321, 660, 1737, 2132, 2528, 2801, 2847
BsuTUI	ATCGAT	1 cut(s)	225
BtgI	CCRYGG	2 cut(s)	1272, 2904
BtrI	CACGTC	2 cut(s)	184, 3136
BtsI	GCAGTG	2 cut(s)	2333, 2790
Cac8I	GCNNGC	8 cut(s)	245, 252, 1205, 1321, 1656, 2212, 2694, 3190
CfoI	GCGC	3 cut(s)	1236, 2212, 2214
Cfr10I	RCCGGY	3 cut(s)	243, 2759, 2914
Cfr13I	GGNCC	4 cut(s)	319, 689, 1455, 1736
ClaI	ATCGAT	1 cut(s)	225
CseI	GACGC	1 cut(s)	75
Csp6I	GTAC	8 cut(s)	66, 314, 936, 1262, 1875, 1974, 2289, 2918
CspCI	CAANNNNNGTGG	2 cut(s)	2791, 2826
CviQI	GTAC	8 cut(s)	66, 314, 936, 1262, 1875, 1974, 2289, 2918
EaeI	YGGCCR	4 cut(s)	132, 262, 2799, 2845
Eam1104I	CTCTTC	3 cut(s)	1137, 1349, 1601
EarI	CTCTTC	3 cut(s)	1137, 1349, 1601
Eco130I	CCWWGG	6 cut(s)	872, 1272, 1567, 2142, 2904, 2925
Eco147I	AGGCCT	1 cut(s)	660
Eco24I	GRGCYC	2 cut(s)	2023, 2299
Eco31I	GGTCTC	1 cut(s)	10
Eco32I	GATATC	2 cut(s)	223, 574
Eco47I	GGWCC	2 cut(s)	689, 1455
Eco57I	CTGAAG	2 cut(s)	2292, 2475
Eco81I	CCTNAGG	3 cut(s)	925, 2727, 2978
EcoNI	CCTNNNNNAGG	1 cut(s)	24
EcoO109I	RGGNCCY	1 cut(s)	319
EcoRI	GAATTC	2 cut(s)	596, 695
EcoRII	CCWGG	8 cut(s)	552, 1021, 1817, 2132, 2204, 2617, 2817, 2940
EcoRV	GATATC	2 cut(s)	223, 574
EcoT14I	CCWWGG	6 cut(s)	872, 1272, 1567, 2142, 2904, 2925
EcoT22I	ATGCAT	1 cut(s)	2074
EcoT38I	GRGCYC	2 cut(s)	2023, 2299
ErhI	CCWWGG	6 cut(s)	872, 1272, 1567, 2142, 2904, 2925
Esp3I	CGTCTC	3 cut(s)	33, 263, 1709
FaqI	GGGAC	6 cut(s)	403, 1131, 1254, 2150, 2462, 3042
FauI	CCCGC	1 cut(s)	155
FauNDI	CATATG	1 cut(s)	2673
FbaI	TGATCA	3 cut(s)	93, 1831, 2002
FblI	GTMKAC	1 cut(s)	1664
FriOI	GRGCYC	2 cut(s)	2023, 2299
FspBI	CTAG	7 cut(s)	9, 891, 1802, 1941, 2286, 2709, 2885
GlaI	GCGC	3 cut(s)	1235, 2211, 2213
GsaI	CCCAGC	2 cut(s)	2121, 2691
GsuI	CTGGAG	4 cut(s)	1496, 1935, 2040, 2993
HaeIII	GGCC	9 cut(s)	134, 264, 321, 660, 1737, 2132, 2528, 2801, 2847
HapII	CCGG	8 cut(s)	216, 244, 265, 2242, 2358, 2626, 2760, 2915
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	3 cut(s)	1236, 2212, 2214
Hin6I	GCGC	3 cut(s)	1234, 2210, 2212
HinP1I	GCGC	3 cut(s)	1234, 2210, 2212
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	3 cut(s)	101, 1157, 1317
HpaI	GTTAAC	1 cut(s)	52
HpaII	CCGG	8 cut(s)	216, 244, 265, 2242, 2358, 2626, 2760, 2915
HphI	GGTGA	4 cut(s)	56, 1037, 1907, 2257
Hpy166II	GTNNAC	8 cut(s)	52, 1511, 1665, 1974, 1981, 2333, 2734, 3238
Hpy8I	GTNNAC	8 cut(s)	52, 1511, 1665, 1974, 1981, 2333, 2734, 3238
Hpy99I	CGWCG	1 cut(s)	185
HpyAV	CCTTC	7 cut(s)	20, 29, 380, 646, 1849, 3106, 3136
HpyCH4III	ACNGT	8 cut(s)	44, 158, 394, 703, 1669, 1978, 2009, 2959
HpyCH4IV	ACGT	4 cut(s)	48, 120, 183, 3135
HpySE526I	ACGT	4 cut(s)	48, 120, 183, 3135
HspAI	GCGC	3 cut(s)	1234, 2210, 2212
Kpn2I	TCCGGA	1 cut(s)	2625
KpnI	GGTACC	3 cut(s)	69, 939, 2921
KroI	GCCGGC	1 cut(s)	243
KroNI	GCCGGC	1 cut(s)	245
Ksp22I	TGATCA	3 cut(s)	93, 1831, 2002
KspAI	GTTAAC	1 cut(s)	52
LmnI	GCTCC	6 cut(s)	165, 397, 535, 1387, 2146, 2302
MaeI	CTAG	7 cut(s)	9, 891, 1802, 1941, 2286, 2709, 2885
MaeII	ACGT	4 cut(s)	48, 120, 183, 3135
MaeIII	GTNAC	7 cut(s)	44, 370, 578, 680, 958, 1610, 2512
MfeI	CAATTG	1 cut(s)	2778
MflI	RGATCY	8 cut(s)	232, 817, 1213, 1502, 1945, 2412, 2973, 3121
MhlI	GDGCHC	5 cut(s)	460, 1513, 2023, 2299, 2335
MlsI	TGGCCA	1 cut(s)	2801
MluNI	TGGCCA	1 cut(s)	2801
MlyI	GAGTC	2 cut(s)	1358, 1877
MmeI	TCCRAC	4 cut(s)	234, 1406, 1480, 2262
Mox20I	TGGCCA	1 cut(s)	2801
Mph1103I	ATGCAT	1 cut(s)	2074
MroI	TCCGGA	1 cut(s)	2625
MroNI	GCCGGC	1 cut(s)	243
MroXI	GAANNNNTTC	1 cut(s)	1377
MscI	TGGCCA	1 cut(s)	2801
MslI	CAYNNNNRTG	6 cut(s)	965, 1516, 1901, 1969, 2795, 3174
Msp20I	TGGCCA	1 cut(s)	2801
MspA1I	CMGCKG	3 cut(s)	1837, 2060, 2254
MspCI	CTTAAG	1 cut(s)	3017
MspI	CCGG	8 cut(s)	216, 244, 265, 2242, 2358, 2626, 2760, 2915
MunI	CAATTG	1 cut(s)	2778
Mva1269I	GAATGC	3 cut(s)	2074, 2721, 3040
MvaI	CCWGG	8 cut(s)	554, 1023, 1819, 2134, 2206, 2619, 2819, 2942
MvnI	CGCG	1 cut(s)	2212
NaeI	GCCGGC	1 cut(s)	245
NciI	CCSGG	3 cut(s)	266, 2243, 2358
NcoI	CCATGG	2 cut(s)	1272, 2904
NdeI	CATATG	1 cut(s)	2673
NgoMIV	GCCGGC	1 cut(s)	243
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	5 cut(s)	44, 370, 680, 958, 2512
NsiI	ATGCAT	1 cut(s)	2074
NspI	RCATGY	1 cut(s)	3082
NspV	TTCGAA	1 cut(s)	2646
OliI	CACNNNNGTG	2 cut(s)	965, 1969
PalAI	GGCGCGCC	1 cut(s)	2210
PauI	GCGCGC	1 cut(s)	2210
PceI	AGGCCT	1 cut(s)	660
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	3 cut(s)	2074, 2721, 3040
PdiI	GCCGGC	1 cut(s)	245
PdmI	GAANNNNTTC	1 cut(s)	1377
PfeI	GAWTC	9 cut(s)	113, 128, 206, 1018, 1122, 1373, 1397, 2322, 2638
PflMI	CCANNNNNTGG	1 cut(s)	2854
PleI	GAGTC	2 cut(s)	1358, 1876
PpsI	GAGTC	2 cut(s)	1358, 1876
PshBI	ATTAAT	3 cut(s)	1182, 1794, 2571
Psp6I	CCWGG	8 cut(s)	552, 1021, 1817, 2132, 2204, 2617, 2817, 2940
PspFI	CCCAGC	2 cut(s)	2117, 2687
PspGI	CCWGG	8 cut(s)	552, 1021, 1817, 2132, 2204, 2617, 2817, 2940
PspPI	GGNCC	4 cut(s)	319, 689, 1455, 1736
PstI	CTGCAG	1 cut(s)	2340
PsuI	RGATCY	8 cut(s)	232, 817, 1213, 1502, 1945, 2412, 2973, 3121
PteI	GCGCGC	1 cut(s)	2210
PvuII	CAGCTG	1 cut(s)	2254
RsaI	GTAC	8 cut(s)	67, 315, 937, 1263, 1876, 1975, 2290, 2919
RsaNI	GTAC	8 cut(s)	66, 314, 936, 1262, 1875, 1974, 2289, 2918
RseI	CAYNNNNRTG	6 cut(s)	965, 1516, 1901, 1969, 2795, 3174
Sau96I	GGNCC	4 cut(s)	319, 689, 1455, 1736
ScaI	AGTACT	2 cut(s)	1263, 2290
SchI	GAGTC	2 cut(s)	1358, 1877
SduI	GDGCHC	5 cut(s)	460, 1513, 2023, 2299, 2335
SfcI	CTRYAG	5 cut(s)	1463, 1665, 2336, 2550, 2863
SfuI	TTCGAA	1 cut(s)	2646
SgsI	GGCGCGCC	1 cut(s)	2210
SinI	GGWCC	2 cut(s)	689, 1455
SmiMI	CAYNNNNRTG	6 cut(s)	965, 1516, 1901, 1969, 2795, 3174
SmlI	CTYRAG	7 cut(s)	911, 1013, 1865, 2045, 2156, 2216, 3017
SmoI	CTYRAG	7 cut(s)	911, 1013, 1865, 2045, 2156, 2216, 3017
SpeI	ACTAGT	1 cut(s)	2285
SseBI	AGGCCT	1 cut(s)	660
SsiI	CCGC	8 cut(s)	77, 162, 533, 626, 1674, 1837, 2060, 2844
SspMI	CTAG	7 cut(s)	9, 891, 1802, 1941, 2286, 2709, 2885
StuI	AGGCCT	1 cut(s)	660
StyI	CCWWGG	6 cut(s)	872, 1272, 1567, 2142, 2904, 2925
TaaI	ACNGT	8 cut(s)	44, 158, 394, 703, 1669, 1978, 2009, 2959
TaiI	ACGT	4 cut(s)	51, 123, 186, 3138
TaqI	TCGA	7 cut(s)	111, 225, 786, 2023, 2602, 2646, 3088
TaqII	GACCGA	1 cut(s)	3114
TatI	WGTACW	3 cut(s)	1261, 1973, 2288
TauI	GCSGC	4 cut(s)	80, 628, 1676, 2847
TfiI	GAWTC	9 cut(s)	113, 128, 206, 1018, 1122, 1373, 1397, 2322, 2638
TseFI	GTSAC	5 cut(s)	44, 370, 680, 958, 2512
Tsp45I	GTSAC	5 cut(s)	44, 370, 680, 958, 2512
TspGWI	ACGGA	3 cut(s)	1400, 1625, 3233
Van91I	CCANNNNNTGG	1 cut(s)	2854
Vha464I	CTTAAG	1 cut(s)	3017
VneI	GTGCAC	2 cut(s)	1509, 2331
VpaK11BI	GGWCC	2 cut(s)	689, 1455
VspI	ATTAAT	3 cut(s)	1182, 1794, 2571
XagI	CCTNNNNNAGG	1 cut(s)	24
XapI	RAATTY	6 cut(s)	596, 695, 803, 1100, 1777, 3150
XbaI	TCTAGA	1 cut(s)	1940
XceI	RCATGY	1 cut(s)	3082
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1244, 1492, 2140, 2424, 2671
XmiI	GTMKAC	1 cut(s)	1664
XmnI	GAANNNNTTC	1 cut(s)	1377
XspI	CTAG	7 cut(s)	9, 891, 1802, 1941, 2286, 2709, 2885
ZrmI	AGTACT	2 cut(s)	1263, 2290
Zsp2I	ATGCAT	1 cut(s)	2074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.