FvH4_2g02190

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009647
Physical Location & Seq
Forward (+)
1819443 .. 1821443
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02190.t2

Sequence Viewer

Length: 957 bp
ATGGCTCAAATCAACAGGCCAGCCATGGAAGAAGATAAAGTCCATGCTTTCTCTTCCCCCATGCCATTGATGTCAGAACATTGGAAGACTGAAGAAGGGAAGAAATCCAGTCGTCCCTCCTCTCTCAGTGAGGTTCTGGGTTTGCGGGAGTTATTTTGTCCCCAGGTGTGGAGAGCGTCTCTAGCTGAGCTTGTCGGCACAGCAGTGCTTGTTTTTGCTATAGACACAATTGTGATCTCCTCCATTGAGACCAATACCTCATCACCAAACCTTCTCCTCTCAATCCTTGTAGCCATCATCGTGGCGATTCTCCTTATCGCTACCAACCCGGTTTCCGGAGGCCACATCAACCCCGTCATCACCTTTTCCGCAGCAACGGTCGGCCTCATTTCCTTCTCACGCGCCGCTGTGTACATTTTCATGCAATGCGTTGGAGGGGTGCTAGGTGCCTTGGCACTCAAGGCTGTGGTCAATAGTAGCCTTCAAGATACATTCTCACTTGGAGGTTGCACCCTAACCGTGGTGGCCCCAGGGCCACAAGGACCCGTCACGATTGGGATCGAGACAGGCCAGGCCCTTTGGCTCGAGGTTATTTGCACGTTTATTTTTCTCTTTGCTTCGATATGGATAGCATTTGACCACCGTCAAGCCCATCCCATGGGTAAGGTTATGGTGCTCTCTATCGTCGGAATAGTGGTCGGTATTCTGGTGTTTATCTCCACCTCGGTAACAACCGTAAAGGGCTACGCCGGGGCTGGGATGAACCCGGCTAGATGCCTCGGCCCGGCACTTGTCCGCGGCGGCCACCTCTGGACGGGGCATTGGGTATTTTGGGTTGGTCCTACTATTGCGTGCTTGGCGTTCTACTTGTACGTCAAGCTCATACCCCGTCAACATTTTCATGTGGAGGGCTACAAGCATGATATCTTCGACTATATGAAGAATCTTCCCAAATAG

Protein Analysis

319

Amino Acids

34.26

Weight (kDa)

8.21

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 54 - 285 2.1e-38 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 658
AccII CGCG 2 cut(s) 402, 798
AccIII TCCGGA 1 cut(s) 335
AciI CCGC 6 cut(s) 145, 369, 405, 796, 798, 801
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 802
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 2 cut(s) 413, 872
AfiI CCNNNNNNNGG 7 cut(s) 168, 335, 520, 658, 756, 784, 814
AgsI TTSAA 1 cut(s) 485
AjnI CCWGG 3 cut(s) 162, 529, 570
AleI CACNNNNGTG 2 cut(s) 203, 230
AluBI AGCT 3 cut(s) 185, 190, 880
AluI AGCT 3 cut(s) 185, 190, 880
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 3 cut(s) 183, 242, 557
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 335
AoxI GGCC 9 cut(s) 17, 340, 382, 525, 533, 568, 573, 781, 802
ApeKI GCWGC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 6 cut(s) 526, 533, 542, 574, 782, 839
AsuC2I CCSGG 4 cut(s) 329, 751, 767, 785
AsuHPI GGTGA 2 cut(s) 255, 352
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 542, 839
BanI GGYRCC 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 1 cut(s) 383
BccI CCATC 2 cut(s) 302, 660
BciT130I CCWGG 3 cut(s) 164, 531, 572
BcnI CCSGG 4 cut(s) 329, 751, 767, 785
BcoDI GTCTC 3 cut(s) 183, 242, 557
BfaI CTAG 3 cut(s) 182, 443, 771
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 4 cut(s) 372, 405, 799, 802
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 4 cut(s) 373, 406, 800, 803
Bme1390I CCNGG 7 cut(s) 164, 329, 531, 572, 751, 767, 785
Bme18I GGWCC 2 cut(s) 542, 839
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 6 cut(s) 526, 533, 542, 574, 782, 839
BmiI GGNNCC 3 cut(s) 448, 528, 544
BmrFI CCNGG 7 cut(s) 164, 329, 531, 572, 751, 767, 785
BmsI GCATC 1 cut(s) 764
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 163, 195
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 4 cut(s) 329, 751, 767, 785
BsaBI GATNNNNATC 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 335
Bsc4I CCNNNNNNNGG 7 cut(s) 168, 335, 520, 658, 756, 784, 814
Bse1I ACTGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 431
Bse8I GATNNNNATC 1 cut(s) 557
BseAI TCCGGA 1 cut(s) 335
BseBI CCWGG 3 cut(s) 164, 531, 572
BseGI GGATG 2 cut(s) 652, 765
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 7 cut(s) 168, 335, 520, 658, 756, 784, 814
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 139, 177
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 3 cut(s) 109, 229, 266
BseXI GCAGC 1 cut(s) 383
BseYI CCCAGC 1 cut(s) 755
Bsh1236I CGCG 2 cut(s) 402, 798
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 9 cut(s) 19, 342, 384, 527, 535, 570, 575, 783, 804
BshNI GGYRCC 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 678
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 5 cut(s) 329, 336, 750, 767, 785
BslFI GGGAC 2 cut(s) 99, 144
BslI CCNNNNNNNGG 7 cut(s) 168, 335, 520, 658, 756, 784, 814
BsmAI GTCTC 3 cut(s) 183, 242, 557
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 2 cut(s) 99, 144
BsnI GGCC 9 cut(s) 19, 342, 384, 527, 535, 570, 575, 783, 804
Bso31I GGTCTC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 678
Bsp13I TCCGGA 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 234, 558
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 2 cut(s) 24, 657
BspACI CCGC 6 cut(s) 145, 369, 405, 796, 798, 801
BspANI GGCC 9 cut(s) 19, 342, 384, 527, 535, 570, 575, 783, 804
BspCNI CTCAG 2 cut(s) 138, 178
BspEI TCCGGA 1 cut(s) 335
BspFNI CGCG 2 cut(s) 402, 798
BspLI GGNNCC 3 cut(s) 448, 528, 544
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 1 cut(s) 108
BssMI GATC 2 cut(s) 234, 558
BssT1I CCWWGG 3 cut(s) 24, 450, 657
Bst2UI CCWGG 3 cut(s) 164, 531, 572
Bst4CI ACNGT 4 cut(s) 379, 520, 644, 736
Bst6I CTCTTC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 411
BstC8I GCNNGC 2 cut(s) 21, 853
BstDEI CTNAG 2 cut(s) 125, 186
BstDSI CCRYGG 4 cut(s) 24, 519, 657, 796
BstF5I GGATG 2 cut(s) 652, 765
BstFNI CGCG 2 cut(s) 402, 798
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 2 cut(s) 237, 561
BstMAI GTCTC 3 cut(s) 183, 242, 557
BstMBI GATC 2 cut(s) 234, 558
BstMCI CGRYCG 1 cut(s) 381
BstMWI GCNNNNNNNGC 3 cut(s) 182, 461, 857
BstNI CCWGG 3 cut(s) 164, 531, 572
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 7 cut(s) 162, 327, 529, 570, 749, 765, 783
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 2 cut(s) 402, 798
BstV1I GCAGC 1 cut(s) 383
BstV2I GAAGAC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuRI GGCC 9 cut(s) 19, 342, 384, 527, 535, 570, 575, 783, 804
BtgI CCRYGG 4 cut(s) 24, 519, 657, 796
BtsCI GGATG 2 cut(s) 652, 765
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 133, 210
Cac8I GCNNGC 2 cut(s) 21, 853
CfoI GCGC 1 cut(s) 404
Cfr13I GGNCC 6 cut(s) 526, 533, 542, 574, 782, 839
Cfr42I CCGCGG 1 cut(s) 799
CseI GACGC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 412, 871
CviAII CATG 7 cut(s) 25, 44, 61, 421, 658, 902, 920
CviQI GTAC 2 cut(s) 412, 871
DdeI CTNAG 2 cut(s) 125, 186
DpnI GATC 2 cut(s) 236, 560
DpnII GATC 2 cut(s) 234, 558
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco130I CCWWGG 3 cut(s) 24, 450, 657
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 542, 839
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 584
EcoO109I RGGNCCY 2 cut(s) 542, 574
EcoRII CCWGG 3 cut(s) 162, 529, 570
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 24, 450, 657
ErhI CCWWGG 3 cut(s) 24, 450, 657
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 7 cut(s) 28, 47, 64, 424, 661, 905, 923
FaqI GGGAC 2 cut(s) 99, 144
FatI CATG 7 cut(s) 24, 43, 60, 420, 657, 901, 919
FauI CCCGC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 372, 405, 799, 802
FokI GGATG 2 cut(s) 639, 772
Fsp4HI GCNGC 4 cut(s) 372, 405, 799, 802
FspBI CTAG 3 cut(s) 182, 443, 771
GlaI GCGC 1 cut(s) 403
GluI GCNGC 4 cut(s) 372, 405, 799, 802
GsaI CCCAGC 1 cut(s) 759
HaeIII GGCC 9 cut(s) 19, 342, 384, 527, 535, 570, 575, 783, 804
HapII CCGG 5 cut(s) 329, 336, 750, 767, 785
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 404
Hin1II CATG 7 cut(s) 28, 47, 64, 424, 661, 905, 923
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 1 cut(s) 893
HindII GTYRAC 1 cut(s) 893
HinfI GANTC 2 cut(s) 307, 943
HpaII CCGG 5 cut(s) 329, 336, 750, 767, 785
HphI GGTGA 2 cut(s) 255, 352
Hpy166II GTNNAC 2 cut(s) 412, 893
Hpy188I TCNGA 2 cut(s) 76, 689
Hpy188III TCNNGA 5 cut(s) 336, 485, 550, 562, 811
Hpy8I GTNNAC 2 cut(s) 412, 893
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 4 cut(s) 89, 281, 403, 491
HpyCH4III ACNGT 4 cut(s) 379, 520, 644, 736
HpyCH4IV ACGT 2 cut(s) 599, 873
HpyCH4V TGCA 3 cut(s) 424, 510, 597
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 461, 857
HpyF3I CTNAG 2 cut(s) 125, 186
HpySE526I ACGT 2 cut(s) 599, 873
Hsp92II CATG 7 cut(s) 28, 47, 64, 424, 661, 905, 923
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 335
KspI CCGCGG 1 cut(s) 799
Kzo9I GATC 2 cut(s) 234, 558
Lsp1109I GCAGC 1 cut(s) 383
LweI GCATC 1 cut(s) 764
MaeI CTAG 3 cut(s) 182, 443, 771
MaeII ACGT 2 cut(s) 599, 873
MaeIII GTNAC 2 cut(s) 547, 727
MalI GATC 2 cut(s) 236, 560
MboI GATC 2 cut(s) 234, 558
MboII GAAGA 9 cut(s) 41, 44, 45, 97, 104, 112, 919, 938, 952
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 1 cut(s) 678
MluCI AATT 1 cut(s) 228
MmeI TCCRAC 2 cut(s) 412, 667
MroI TCCGGA 1 cut(s) 335
MslI CAYNNNNRTG 5 cut(s) 203, 230, 299, 419, 900
MspA1I CMGCKG 2 cut(s) 407, 798
MspI CCGG 5 cut(s) 329, 336, 750, 767, 785
MspR9I CCNGG 7 cut(s) 164, 329, 531, 572, 751, 767, 785
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 3 cut(s) 164, 531, 572
MvnI CGCG 2 cut(s) 402, 798
MwoI GCNNNNNNNGC 3 cut(s) 182, 461, 857
NciI CCSGG 4 cut(s) 329, 751, 767, 785
NcoI CCATGG 2 cut(s) 24, 657
NdeII GATC 2 cut(s) 234, 558
NlaIII CATG 7 cut(s) 28, 47, 64, 424, 661, 905, 923
NlaIV GGNNCC 3 cut(s) 448, 528, 544
NmeAIII GCCGAG 1 cut(s) 759
NmuCI GTSAC 1 cut(s) 547
OliI CACNNNNGTG 2 cut(s) 203, 230
PaeR7I CTCGAG 1 cut(s) 584
PasI CCCWGGG 1 cut(s) 530
PfeI GAWTC 2 cut(s) 307, 943
PflMI CCANNNNNTGG 1 cut(s) 658
PkrI GCNGC 4 cut(s) 373, 406, 800, 803
PpuMI RGGWCCY 1 cut(s) 542
PshAI GACNNNNGTC 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 162, 529, 570
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 3 cut(s) 162, 529, 570
PspN4I GGNNCC 3 cut(s) 448, 528, 544
PspPI GGNCC 6 cut(s) 526, 533, 542, 574, 782, 839
PspPPI RGGWCCY 1 cut(s) 542
PspXI VCTCGAGB 1 cut(s) 584
RsaI GTAC 2 cut(s) 413, 872
RsaNI GTAC 2 cut(s) 412, 871
RseI CAYNNNNRTG 5 cut(s) 203, 230, 299, 419, 900
SacII CCGCGG 1 cut(s) 799
SatI GCNGC 4 cut(s) 372, 405, 799, 802
Sau3AI GATC 2 cut(s) 234, 558
Sau96I GGNCC 6 cut(s) 526, 533, 542, 574, 782, 839
ScrFI CCNGG 7 cut(s) 164, 329, 531, 572, 751, 767, 785
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 1 cut(s) 764
SfcI CTRYAG 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 584
Sfr303I CCGCGG 1 cut(s) 799
SgrBI CCGCGG 1 cut(s) 799
SinI GGWCC 2 cut(s) 542, 839
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 5 cut(s) 203, 230, 299, 419, 900
SmlI CTYRAG 2 cut(s) 458, 584
SmoI CTYRAG 2 cut(s) 458, 584
Sse9I AATT 1 cut(s) 228
SsiI CCGC 6 cut(s) 145, 369, 405, 796, 798, 801
SspMI CTAG 3 cut(s) 182, 443, 771
StyD4I CCNGG 7 cut(s) 162, 327, 529, 570, 749, 765, 783
StyI CCWWGG 3 cut(s) 24, 450, 657
TaaI ACNGT 4 cut(s) 379, 520, 644, 736
TaiI ACGT 2 cut(s) 602, 876
TaqI TCGA 4 cut(s) 561, 585, 620, 930
TasI AATT 1 cut(s) 228
TatI WGTACW 1 cut(s) 411
TauI GCSGC 3 cut(s) 407, 801, 804
TfiI GAWTC 2 cut(s) 307, 943
TscAI CASTG 2 cut(s) 133, 210
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 4 cut(s) 409, 776, 890, 953
TspRI CASTG 2 cut(s) 133, 210
Van91I CCANNNNNTGG 1 cut(s) 658
VpaK11BI GGWCC 2 cut(s) 542, 839
XhoI CTCGAG 1 cut(s) 584
XspI CTAG 3 cut(s) 182, 443, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.