RH6DG026300

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0009647
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG026300.1

Sequence Viewer

Length: 954 bp
ATGGCTCAAATCAACAGGCCAACGATGGAGGAAGATAAAGTTCATGCTTTCTCTTCCCCCATGCCATTAATGTCAGAACATTGGAAGACTGAAGAAGGGAAGAAATCCAATCCCTCCACTTTGAGTGAGGTTTTGGGCTTGCGCGAGTTATTTTCTCCACAGGTGTGGAGAGCATCTCTAGCTGAGCTGGTTGGCACGGCAGTGCTTGTTTTTGCTATAGACGCAATTGTGATCTCCTCCATTGAGACCAATATCTCAGCACCAAACCTTGTATTGTCTATCCTGGTAGCAATCATCGCGGCGATTCTCCTAATTGCTACCAACCCGGTTTCCGGAGGCCACATCAACCCCGTCATCACCTTTTCCGCAGCACTGGTCGGCATCATTTCCTTCTCACGAGCTGCCGTGTACATTTTCATGCAATGCGTCGGAGGAGTTCTAGGTGCCTTGGCACTCAAGGCTGTGGTTAACAGCAGCCTTGAAAAAACATTCTCACTTGGAGGTTGCACCCTAACCGTGGTTACCCCGGGCCCACAAGGAGCCATCACGGTCGGGATCGAGACAGGCCAGGCCCTTTGGCTCGAGATAATATGCACATTTATTTTCCTCTTTGCTTCGATATGGGTGGCGTTCGACCACCGCCAAGCCCACGCCATGGGTAAGGTTATGGTCCTCTCTATCGTCGGAATAGTGGTGGGCGTTCTTGTGTTTATCTCGACCACTGTAACAACTGTAAAGGGCTACGCTGGGGCTGGAATGAACCCAGCTAGATGTCTCGGCCCAGCACTTGTTCATGGGGGCCACCTCTGGACCGGCCATTGGGTATTTTGGGTGGGGCCTACTATTTCTTGCTTCGCTTTCTACTTGTACGTCAAACTCATTCCCCGTCAACATTTCCATGTGGAGGGCTACAAGCATGATATCTTCAACTATGTGAAGACTCTTCCCAAGTAG

Protein Analysis

317

Amino Acids

34.07

Weight (kDa)

8.27

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 51 - 283 5.1e-39 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 655
AccII CGCG 2 cut(s) 144, 299
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 3 cut(s) 299, 366, 640
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 2 cut(s) 410, 869
AfiI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
AgsI TTSAA 2 cut(s) 482, 928
AjnI CCWGG 2 cut(s) 282, 567
AleI CACNNNNGTG 2 cut(s) 163, 200
AluBI AGCT 4 cut(s) 182, 187, 401, 767
AluI AGCT 4 cut(s) 182, 187, 401, 767
Alw26I GTCTC 3 cut(s) 239, 554, 779
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 526, 581
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 9 cut(s) 17, 337, 529, 565, 570, 778, 799, 814, 836
ApaI GGGCCC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 368, 401, 474
AseI ATTAAT 1 cut(s) 68
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 8 cut(s) 529, 530, 571, 670, 779, 799, 810, 836
AsuC2I CCSGG 3 cut(s) 326, 527, 528
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 2 cut(s) 526, 581
AvaII GGWCC 2 cut(s) 670, 810
BaeGI GKGCMC 1 cut(s) 533
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 533
BauI CACGAG 1 cut(s) 396
BbsI GAAGAC 2 cut(s) 92, 944
BbvI GCAGC 3 cut(s) 380, 388, 486
BccI CCATC 2 cut(s) 19, 551
BceAI ACGGC 2 cut(s) 213, 389
BciT130I CCWGG 2 cut(s) 284, 569
BcnI CCSGG 3 cut(s) 326, 527, 528
BcoDI GTCTC 3 cut(s) 239, 554, 779
BfaI CTAG 3 cut(s) 179, 440, 768
BfmI CTRYAG 1 cut(s) 216
BisI GCNGC 4 cut(s) 300, 369, 402, 475
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 4 cut(s) 301, 370, 403, 476
Bme1390I CCNGG 5 cut(s) 284, 326, 527, 528, 569
Bme18I GGWCC 2 cut(s) 670, 810
BmeT110I CYCGRG 2 cut(s) 526, 581
BmgT120I GGNCC 8 cut(s) 529, 530, 571, 670, 779, 799, 810, 836
BmiI GGNNCC 5 cut(s) 445, 531, 541, 800, 837
BmrFI CCNGG 5 cut(s) 284, 326, 527, 528, 569
BmsI GCATC 2 cut(s) 182, 390
BpiI GAAGAC 2 cut(s) 92, 944
BplI GAGNNNNNCTC 2 cut(s) 160, 192
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 3 cut(s) 326, 527, 528
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 5 cut(s) 447, 516, 525, 526, 654
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 428
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 2 cut(s) 284, 569
BseDI CCNNGG 5 cut(s) 447, 516, 525, 526, 654
BseLI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
BseMI GCAATG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 174, 270
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 226, 447
BseSI GKGCMC 1 cut(s) 533
BseXI GCAGC 3 cut(s) 380, 388, 486
BseYI CCCAGC 3 cut(s) 746, 763, 781
Bsh1236I CGCG 2 cut(s) 144, 299
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 9 cut(s) 19, 339, 531, 567, 572, 780, 801, 816, 838
BshNI GGYRCC 1 cut(s) 443
BsiEI CGRYCG 1 cut(s) 552
BsiHKCI CYCGRG 2 cut(s) 526, 581
BsiSI CCGG 4 cut(s) 326, 333, 527, 813
BslI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
BsmAI GTCTC 3 cut(s) 239, 554, 779
BsnI GGCC 9 cut(s) 19, 339, 531, 567, 572, 780, 801, 816, 838
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 2 cut(s) 526, 581
Bsp120I GGGCCC 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 533
Bsp13I TCCGGA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 2 cut(s) 231, 555
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 299, 366, 640
BspANI GGCC 9 cut(s) 19, 339, 531, 567, 572, 780, 801, 816, 838
BspCNI CTCAG 2 cut(s) 175, 269
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 2 cut(s) 144, 299
BspLI GGNNCC 5 cut(s) 445, 531, 541, 800, 837
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 812
BsrGI TGTACA 1 cut(s) 408
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 5 cut(s) 447, 516, 525, 526, 654
BssMI GATC 2 cut(s) 231, 555
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 2 cut(s) 447, 654
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 284, 569
Bst4CI ACNGT 4 cut(s) 517, 550, 724, 733
Bst6I CTCTTC 2 cut(s) 58, 948
BstAUI TGTACA 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 2 cut(s) 183, 256
BstDSI CCRYGG 2 cut(s) 516, 654
BstEII GGTNACC 1 cut(s) 520
BstFNI CGCG 2 cut(s) 144, 299
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 2 cut(s) 234, 558
BstMAI GTCTC 3 cut(s) 239, 554, 779
BstMBI GATC 2 cut(s) 231, 555
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 4 cut(s) 179, 221, 296, 458
BstNI CCWGG 2 cut(s) 284, 569
BstPI GGTNACC 1 cut(s) 520
BstSCI CCNGG 5 cut(s) 282, 324, 525, 526, 567
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 1 cut(s) 533
BstUI CGCG 2 cut(s) 144, 299
BstV1I GCAGC 3 cut(s) 380, 388, 486
BstV2I GAAGAC 2 cut(s) 92, 944
BstXI CCANNNNNNTGG 1 cut(s) 165
BsuRI GGCC 9 cut(s) 19, 339, 531, 567, 572, 780, 801, 816, 838
BtgI CCRYGG 2 cut(s) 516, 654
BtgZI GCGATG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 207, 371, 720
Cac8I GCNNGC 1 cut(s) 140
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 8 cut(s) 529, 530, 571, 670, 779, 799, 810, 836
Cfr9I CCCGGG 1 cut(s) 526
CseI GACGC 2 cut(s) 230, 415
Csp6I GTAC 2 cut(s) 409, 868
CviAII CATG 7 cut(s) 44, 61, 418, 655, 794, 899, 917
CviQI GTAC 2 cut(s) 409, 868
DdeI CTNAG 2 cut(s) 183, 256
DpnI GATC 2 cut(s) 233, 557
DpnII GATC 2 cut(s) 231, 555
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 2 cut(s) 58, 948
EarI CTCTTC 2 cut(s) 58, 948
Eco130I CCWWGG 2 cut(s) 447, 654
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 1 cut(s) 239
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 670, 810
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 2 cut(s) 526, 581
Eco91I GGTNACC 1 cut(s) 520
EcoO109I RGGNCCY 2 cut(s) 571, 836
EcoO65I GGTNACC 1 cut(s) 520
EcoRII CCWGG 2 cut(s) 282, 567
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 2 cut(s) 447, 654
EcoT38I GRGCYC 1 cut(s) 533
ErhI CCWWGG 2 cut(s) 447, 654
FaeI CATG 7 cut(s) 47, 64, 421, 658, 797, 902, 920
FatI CATG 7 cut(s) 43, 60, 417, 654, 793, 898, 916
Fnu4HI GCNGC 4 cut(s) 300, 369, 402, 475
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 4 cut(s) 300, 369, 402, 475
FspBI CTAG 3 cut(s) 179, 440, 768
GlaI GCGC 1 cut(s) 143
GluI GCNGC 4 cut(s) 300, 369, 402, 475
GsaI CCCAGC 3 cut(s) 750, 767, 785
HaeIII GGCC 9 cut(s) 19, 339, 531, 567, 572, 780, 801, 816, 838
HapII CCGG 4 cut(s) 326, 333, 527, 813
HgaI GACGC 2 cut(s) 230, 415
HhaI GCGC 1 cut(s) 144
Hin1II CATG 7 cut(s) 47, 64, 421, 658, 797, 902, 920
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 2 cut(s) 469, 890
HindII GTYRAC 2 cut(s) 469, 890
HinfI GANTC 2 cut(s) 304, 940
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 4 cut(s) 326, 333, 527, 813
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 3 cut(s) 409, 469, 890
Hpy188I TCNGA 3 cut(s) 76, 431, 686
Hpy188III TCNNGA 7 cut(s) 333, 396, 553, 559, 583, 715, 808
Hpy8I GTNNAC 3 cut(s) 409, 469, 890
Hpy99I CGWCG 2 cut(s) 431, 686
HpyAV CCTTC 2 cut(s) 89, 400
HpyCH4III ACNGT 4 cut(s) 517, 550, 724, 733
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 3 cut(s) 421, 507, 594
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 221, 296, 458
HpyF3I CTNAG 2 cut(s) 183, 256
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 7 cut(s) 47, 64, 421, 658, 797, 902, 920
HspAI GCGC 1 cut(s) 142
Kpn2I TCCGGA 1 cut(s) 332
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 2 cut(s) 231, 555
LmnI GCTCC 1 cut(s) 539
Lsp1109I GCAGC 3 cut(s) 380, 388, 486
LweI GCATC 2 cut(s) 182, 390
MaeI CTAG 3 cut(s) 179, 440, 768
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 2 cut(s) 520, 724
MalI GATC 2 cut(s) 233, 557
MboI GATC 2 cut(s) 231, 555
MboII GAAGA 8 cut(s) 44, 45, 97, 104, 112, 916, 935, 949
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 533
MluCI AATT 2 cut(s) 225, 312
MlyI GAGTC 1 cut(s) 934
MmeI TCCRAC 2 cut(s) 409, 664
MroI TCCGGA 1 cut(s) 332
MseI TTAA 2 cut(s) 68, 468
MslI CAYNNNNRTG 4 cut(s) 163, 200, 416, 897
MspI CCGG 4 cut(s) 326, 333, 527, 813
MspR9I CCNGG 5 cut(s) 284, 326, 527, 528, 569
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 2 cut(s) 284, 569
MvnI CGCG 2 cut(s) 144, 299
MwoI GCNNNNNNNGC 4 cut(s) 179, 221, 296, 458
NciI CCSGG 3 cut(s) 326, 527, 528
NcoI CCATGG 1 cut(s) 654
NdeII GATC 2 cut(s) 231, 555
NlaIII CATG 7 cut(s) 47, 64, 421, 658, 797, 902, 920
NlaIV GGNNCC 5 cut(s) 445, 531, 541, 800, 837
NmeAIII GCCGAG 1 cut(s) 756
OliI CACNNNNGTG 2 cut(s) 163, 200
PaeR7I CTCGAG 1 cut(s) 581
PfeI GAWTC 1 cut(s) 304
PflMI CCANNNNNTGG 1 cut(s) 655
PkrI GCNGC 4 cut(s) 301, 370, 403, 476
PleI GAGTC 1 cut(s) 934
PpsI GAGTC 1 cut(s) 934
PshBI ATTAAT 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 282, 567
PspEI GGTNACC 1 cut(s) 520
PspFI CCCAGC 3 cut(s) 746, 763, 781
PspGI CCWGG 2 cut(s) 282, 567
PspN4I GGNNCC 5 cut(s) 445, 531, 541, 800, 837
PspOMI GGGCCC 1 cut(s) 529
PspPI GGNCC 8 cut(s) 529, 530, 571, 670, 779, 799, 810, 836
RsaI GTAC 2 cut(s) 410, 869
RsaNI GTAC 2 cut(s) 409, 868
RseI CAYNNNNRTG 4 cut(s) 163, 200, 416, 897
SaqAI TTAA 2 cut(s) 68, 468
SatI GCNGC 4 cut(s) 300, 369, 402, 475
Sau3AI GATC 2 cut(s) 231, 555
Sau96I GGNCC 8 cut(s) 529, 530, 571, 670, 779, 799, 810, 836
SchI GAGTC 1 cut(s) 934
ScrFI CCNGG 5 cut(s) 284, 326, 527, 528, 569
SduI GDGCHC 1 cut(s) 533
SfaNI GCATC 2 cut(s) 182, 390
SfcI CTRYAG 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 2 cut(s) 670, 810
SlaI CTCGAG 1 cut(s) 581
SmaI CCCGGG 1 cut(s) 528
SmiMI CAYNNNNRTG 4 cut(s) 163, 200, 416, 897
SmlI CTYRAG 2 cut(s) 455, 581
SmoI CTYRAG 2 cut(s) 455, 581
Sse9I AATT 2 cut(s) 225, 312
SsiI CCGC 3 cut(s) 299, 366, 640
SspMI CTAG 3 cut(s) 179, 440, 768
StyD4I CCNGG 5 cut(s) 282, 324, 525, 526, 567
StyI CCWWGG 2 cut(s) 447, 654
TaaI ACNGT 4 cut(s) 517, 550, 724, 733
TaiI ACGT 1 cut(s) 873
TaqI TCGA 5 cut(s) 558, 582, 617, 633, 716
TasI AATT 2 cut(s) 225, 312
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 2 cut(s) 68, 468
Tru9I TTAA 2 cut(s) 68, 468
TscAI CASTG 3 cut(s) 207, 378, 727
TseI GCWGC 3 cut(s) 368, 401, 474
TspDTI ATGAA 4 cut(s) 32, 406, 773, 782
TspMI CCCGGG 1 cut(s) 526
TspRI CASTG 3 cut(s) 207, 378, 727
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 670, 810
VspI ATTAAT 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 581
XmaI CCCGGG 1 cut(s) 526
XspI CTAG 3 cut(s) 179, 440, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.