RLG00000015294

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009647
Physical Location & Seq
Reverse (-)
64134729 .. 64136384
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015294

Sequence Viewer

Length: 954 bp
ATGGCTCAAATCAACAGGCCAACGATGGAGGAAGATAAAGTTCATGCTTTCTCTTCCCCCATGCCATTAATGTCAGAACATTGGAAGACTGAAGAAGGGAAGAAATCCAATCCCTCCAATTTGAGTGAGGTTTTGGGCTTGCGCGAGTTATTTTCTCCACAGGTGTGGAGAGCATCTCTAGCTGAGCTGGTTGGCACGGCAGTGCTTGTTTTTGCAATAGACGCAATTGTGATCTCCTCCATTGAGACCAATATCTCAGCACCAAACCTTGTATTGTCAATCCTGGTAGCAATCACCGTGGCGATTCTCCTGATTGCTACCAACCCGGTTTCCGGAGGCCACATCAACCCCGTCATCACCTTTTCCGCAGCACTGGTCGGCATCATTTCCTTCTCACGCGCTGCTGTGTACATTTTCATGCAATGCGTCGGAGGAGTTCTAGGTGCCTTGGCGCTCAAGGCTGTGGTTAACAGCAGCCTTGAAGAAACATTCTCGCTTGGAGGTTGCACCCTAACCGTGGTTTCTCCGGGCCCACAAGGGCCCATAACGGTCGGGATCGAGACAGGCCAGGCCCTTTGGCTCGAGATAATATGCACATTTATTTTCCTCTTTTCTTCGATATGGATGGCGTTCGACCACCGCCAAGCCCACGCCATGGGTAAGGTTATGGTCCTCTCTATCGTCGGAATAGTGGTGGGCATTCTTGTGTTTATCTCGACCTCTGTAACAACTGTGAAGGGCTACGCTGGGGCTGGAATGAACCCAGCTAGATGTCTCGGCCCAGCACTTGTTCGTGGGGGCCACCTCTGGACCGGCCACTGGGTATTTTGGGTCGGGCCTACTATTTCTTGCTTCGCTTTCTACTTGTACGTCAAGCTCATTCCCCGTCAACATTTCCATGTGGAGGGCTACAAGCATGATATCTTCAACTATGTGAAGTCTCTTCCCAAGTAG

Protein Analysis

318

Amino Acids

34.17

Weight (kDa)

7.73

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 51 - 283 2.4e-39 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 655
AccII CGCG 2 cut(s) 144, 399
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 2 cut(s) 366, 640
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 814
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 2 cut(s) 410, 869
AfiI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
AgsI TTSAA 2 cut(s) 482, 928
AjnI CCWGG 2 cut(s) 282, 567
AleI CACNNNNGTG 2 cut(s) 163, 200
AluBI AGCT 4 cut(s) 182, 187, 767, 877
AluI AGCT 4 cut(s) 182, 187, 767, 877
Alw26I GTCTC 4 cut(s) 239, 554, 779, 945
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 581
Aor13HI TCCGGA 1 cut(s) 332
ApaI GGGCCC 2 cut(s) 533, 543
ApeKI GCWGC 3 cut(s) 368, 401, 474
AseI ATTAAT 1 cut(s) 68
AspLEI GCGC 3 cut(s) 144, 401, 454
AsuC2I CCSGG 2 cut(s) 326, 528
AsuHPI GGTGA 2 cut(s) 286, 349
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 2 cut(s) 670, 810
BaeGI GKGCMC 2 cut(s) 533, 543
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 2 cut(s) 533, 543
BbsI GAAGAC 1 cut(s) 92
BbvI GCAGC 3 cut(s) 380, 388, 486
BccI CCATC 2 cut(s) 19, 619
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 2 cut(s) 284, 569
BcnI CCSGG 2 cut(s) 326, 528
BcoDI GTCTC 4 cut(s) 239, 554, 779, 945
BfaI CTAG 3 cut(s) 179, 440, 768
BfoI RGCGCY 1 cut(s) 455
BisI GCNGC 3 cut(s) 369, 402, 475
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 3 cut(s) 370, 403, 476
Bme1390I CCNGG 4 cut(s) 284, 326, 528, 569
Bme18I GGWCC 2 cut(s) 670, 810
BmeT110I CYCGRG 1 cut(s) 581
BmiI GGNNCC 4 cut(s) 445, 531, 541, 800
BmrFI CCNGG 4 cut(s) 284, 326, 528, 569
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 2 cut(s) 182, 390
BmuI ACTGGG 1 cut(s) 829
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 160, 192
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 326, 528
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 297, 447, 516, 654
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 2 cut(s) 378, 824
Bse3DI GCAATG 1 cut(s) 428
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 2 cut(s) 284, 569
BseDI CCNNGG 4 cut(s) 297, 447, 516, 654
BseGI GGATG 1 cut(s) 630
BseLI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
BseMI GCAATG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 174, 270
BseNI ACTGG 2 cut(s) 378, 824
BseRI GAGGAG 2 cut(s) 226, 447
BseSI GKGCMC 2 cut(s) 533, 543
BseXI GCAGC 3 cut(s) 380, 388, 486
BseYI CCCAGC 3 cut(s) 746, 763, 781
Bsh1236I CGCG 2 cut(s) 144, 399
Bsh1285I CGRYCG 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 443
BsiEI CGRYCG 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 4 cut(s) 326, 333, 527, 813
BslI CCNNNNNNNGG 5 cut(s) 332, 517, 655, 819, 904
BsmAI GTCTC 4 cut(s) 239, 554, 779, 945
BsmI GAATGC 1 cut(s) 699
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 581
Bsp120I GGGCCC 2 cut(s) 529, 539
Bsp1286I GDGCHC 2 cut(s) 533, 543
Bsp13I TCCGGA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 2 cut(s) 231, 555
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 2 cut(s) 366, 640
BspCNI CTCAG 2 cut(s) 175, 269
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 2 cut(s) 144, 399
BspLI GGNNCC 4 cut(s) 445, 531, 541, 800
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 812
BsrGI TGTACA 1 cut(s) 408
BsrI ACTGG 2 cut(s) 378, 824
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 4 cut(s) 297, 447, 516, 654
BssMI GATC 2 cut(s) 231, 555
BssT1I CCWWGG 2 cut(s) 447, 654
Bst2UI CCWGG 2 cut(s) 284, 569
Bst4CI ACNGT 4 cut(s) 298, 517, 550, 733
Bst6I CTCTTC 2 cut(s) 58, 948
BstAUI TGTACA 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 2 cut(s) 183, 256
BstDSI CCRYGG 3 cut(s) 297, 516, 654
BstF5I GGATG 1 cut(s) 630
BstFNI CGCG 2 cut(s) 144, 399
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 3 cut(s) 144, 401, 454
BstKTI GATC 2 cut(s) 234, 558
BstMAI GTCTC 4 cut(s) 239, 554, 779, 945
BstMBI GATC 2 cut(s) 231, 555
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 3 cut(s) 179, 221, 458
BstNI CCWGG 2 cut(s) 284, 569
BstSCI CCNGG 4 cut(s) 282, 324, 526, 567
BstSLI GKGCMC 2 cut(s) 533, 543
BstUI CGCG 2 cut(s) 144, 399
BstV1I GCAGC 3 cut(s) 380, 388, 486
BstV2I GAAGAC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 165
BtgI CCRYGG 3 cut(s) 297, 516, 654
BtsCI GGATG 1 cut(s) 630
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 207, 371, 817
Cac8I GCNNGC 1 cut(s) 140
CfoI GCGC 3 cut(s) 144, 401, 454
Cfr10I RCCGGY 1 cut(s) 812
CseI GACGC 2 cut(s) 230, 415
Csp6I GTAC 2 cut(s) 409, 868
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 6 cut(s) 44, 61, 418, 655, 899, 917
CviQI GTAC 2 cut(s) 409, 868
DdeI CTNAG 2 cut(s) 183, 256
DpnI GATC 2 cut(s) 233, 557
DpnII GATC 2 cut(s) 231, 555
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 2 cut(s) 58, 948
EarI CTCTTC 2 cut(s) 58, 948
Eco130I CCWWGG 2 cut(s) 447, 654
Eco24I GRGCYC 2 cut(s) 533, 543
Eco31I GGTCTC 1 cut(s) 239
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 670, 810
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 581
EcoO109I RGGNCCY 2 cut(s) 539, 571
EcoRII CCWGG 2 cut(s) 282, 567
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 2 cut(s) 447, 654
EcoT38I GRGCYC 2 cut(s) 533, 543
ErhI CCWWGG 2 cut(s) 447, 654
FaeI CATG 6 cut(s) 47, 64, 421, 658, 902, 920
FatI CATG 6 cut(s) 43, 60, 417, 654, 898, 916
Fnu4HI GCNGC 3 cut(s) 369, 402, 475
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 2 cut(s) 533, 543
Fsp4HI GCNGC 3 cut(s) 369, 402, 475
FspBI CTAG 3 cut(s) 179, 440, 768
GlaI GCGC 3 cut(s) 143, 400, 453
GluI GCNGC 3 cut(s) 369, 402, 475
GsaI CCCAGC 3 cut(s) 750, 767, 785
HaeII RGCGCY 1 cut(s) 455
HapII CCGG 4 cut(s) 326, 333, 527, 813
HgaI GACGC 2 cut(s) 230, 415
HhaI GCGC 3 cut(s) 144, 401, 454
Hin1II CATG 6 cut(s) 47, 64, 421, 658, 902, 920
Hin6I GCGC 3 cut(s) 142, 399, 452
HinP1I GCGC 3 cut(s) 142, 399, 452
HincII GTYRAC 2 cut(s) 469, 890
HindII GTYRAC 2 cut(s) 469, 890
HinfI GANTC 1 cut(s) 304
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 4 cut(s) 326, 333, 527, 813
HphI GGTGA 2 cut(s) 286, 349
Hpy166II GTNNAC 3 cut(s) 409, 469, 890
Hpy188I TCNGA 3 cut(s) 76, 431, 686
Hpy188III TCNNGA 7 cut(s) 310, 333, 553, 559, 583, 715, 808
Hpy8I GTNNAC 3 cut(s) 409, 469, 890
Hpy99I CGWCG 2 cut(s) 431, 686
HpyAV CCTTC 3 cut(s) 89, 400, 730
HpyCH4III ACNGT 4 cut(s) 298, 517, 550, 733
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 4 cut(s) 215, 421, 507, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 221, 458
HpyF3I CTNAG 2 cut(s) 183, 256
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 6 cut(s) 47, 64, 421, 658, 902, 920
HspAI GCGC 3 cut(s) 142, 399, 452
Kpn2I TCCGGA 1 cut(s) 332
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 2 cut(s) 231, 555
Lsp1109I GCAGC 3 cut(s) 380, 388, 486
LweI GCATC 2 cut(s) 182, 390
MaeI CTAG 3 cut(s) 179, 440, 768
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 1 cut(s) 724
MalI GATC 2 cut(s) 233, 557
MboI GATC 2 cut(s) 231, 555
MboII GAAGA 9 cut(s) 44, 45, 97, 104, 112, 494, 606, 916, 935
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 533, 543
MluCI AATT 2 cut(s) 118, 225
MmeI TCCRAC 2 cut(s) 409, 664
MroI TCCGGA 1 cut(s) 332
MseI TTAA 2 cut(s) 68, 468
MslI CAYNNNNRTG 5 cut(s) 163, 200, 416, 704, 897
MspI CCGG 4 cut(s) 326, 333, 527, 813
MspR9I CCNGG 4 cut(s) 284, 326, 528, 569
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 2 cut(s) 284, 569
MvnI CGCG 2 cut(s) 144, 399
MwoI GCNNNNNNNGC 3 cut(s) 179, 221, 458
NciI CCSGG 2 cut(s) 326, 528
NcoI CCATGG 1 cut(s) 654
NdeII GATC 2 cut(s) 231, 555
NlaIII CATG 6 cut(s) 47, 64, 421, 658, 902, 920
NlaIV GGNNCC 4 cut(s) 445, 531, 541, 800
NmeAIII GCCGAG 1 cut(s) 756
OliI CACNNNNGTG 2 cut(s) 163, 200
PaeR7I CTCGAG 1 cut(s) 581
PctI GAATGC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 304
PflMI CCANNNNNTGG 1 cut(s) 655
PkrI GCNGC 3 cut(s) 370, 403, 476
PshBI ATTAAT 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 282, 567
PspFI CCCAGC 3 cut(s) 746, 763, 781
PspGI CCWGG 2 cut(s) 282, 567
PspN4I GGNNCC 4 cut(s) 445, 531, 541, 800
PspOMI GGGCCC 2 cut(s) 529, 539
RsaI GTAC 2 cut(s) 410, 869
RsaNI GTAC 2 cut(s) 409, 868
RseI CAYNNNNRTG 5 cut(s) 163, 200, 416, 704, 897
SaqAI TTAA 2 cut(s) 68, 468
SatI GCNGC 3 cut(s) 369, 402, 475
Sau3AI GATC 2 cut(s) 231, 555
ScrFI CCNGG 4 cut(s) 284, 326, 528, 569
SduI GDGCHC 2 cut(s) 533, 543
SfaNI GCATC 2 cut(s) 182, 390
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 2 cut(s) 670, 810
SlaI CTCGAG 1 cut(s) 581
SmiMI CAYNNNNRTG 5 cut(s) 163, 200, 416, 704, 897
SmlI CTYRAG 2 cut(s) 455, 581
SmoI CTYRAG 2 cut(s) 455, 581
Sse9I AATT 2 cut(s) 118, 225
SsiI CCGC 2 cut(s) 366, 640
SspMI CTAG 3 cut(s) 179, 440, 768
StyD4I CCNGG 4 cut(s) 282, 324, 526, 567
StyI CCWWGG 2 cut(s) 447, 654
TaaI ACNGT 4 cut(s) 298, 517, 550, 733
TaiI ACGT 1 cut(s) 873
TaqI TCGA 5 cut(s) 558, 582, 617, 633, 716
TasI AATT 2 cut(s) 118, 225
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 2 cut(s) 68, 468
Tru9I TTAA 2 cut(s) 68, 468
TscAI CASTG 3 cut(s) 207, 378, 824
TseI GCWGC 3 cut(s) 368, 401, 474
TspDTI ATGAA 3 cut(s) 32, 406, 773
TspRI CASTG 3 cut(s) 207, 378, 824
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 670, 810
VspI ATTAAT 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 581
XspI CTAG 3 cut(s) 179, 440, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.