RchiOBHm_Chr6g0245821

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009647
Physical Location & Seq
Forward (+)
1568068 .. 1568736
669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22033

Sequence Viewer

Length: 669 bp
ATGATCTGTAGGAAAGCTAGGAGGGAAATAAGGGAGTATGATGACATGGCTAACAACATCAACCCCGTCATCACCTTTTCCGCGGCACTAGTCGGCATCATTTCCTTCTCACGAGCTGCCGTGTACATTTTCATGCAATGCGTGGGAGGAGTTCTAGGTGCCTTGGCACTCAAGGCTGTGATTAACAGCAGCCTTGAAGAAACATTCTCACTTGGAGGTTGCACCCTAACCGTGGTTTCTCCTGACCCACAAGGGCCCATCACGGTCAGGATCGGGACAGGCCAGGCCCTTTGGCTCGAGATAATATGCACATTTATTTCCCTCCTTGCGTCGATATGGATGGCCTTCGACCACCGCCAAGCCCACTCCATGGGTAAGGTTATGGTCCTCTCTATTGTCGGAATAGTGGTGGGCATTCTTGTGTTTATCTCGACCTTTGTAACAGCTGTGAAGGGCTACGCTGGGCCTGGAATAAACCCAGTTAGATGCCTCGGCCCAGCACTTGTTCGTGGGGGCCACCTCTGGATTGGCCACTGGGTTTTTTGGGTTGGGCCTACTATTTCTTGCTTCGCTTTCTACTTATACGTCAAGCTCATTCCCCGTCAACATTTCCATGTGGAGGGCTGCAAGCATGATATCTTCGACTATGTGAAGACTCTTCCCAAGTAG

Protein Analysis

222

Amino Acids

24.33

Weight (kDa)

9.03

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 20 - 188 5.5e-26 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 81, 83, 355
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 529
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 232, 370, 619
AgsI TTSAA 1 cut(s) 197
AhlI ACTAGT 1 cut(s) 88
AjnI CCWGG 2 cut(s) 282, 466
AluBI AGCT 4 cut(s) 17, 116, 446, 592
AluI AGCT 4 cut(s) 17, 116, 446, 592
AlwI GGATC 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 9 cut(s) 254, 280, 285, 342, 464, 493, 514, 529, 551
ApaI GGGCCC 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 116, 189, 624
AspS9I GGNCC 8 cut(s) 254, 255, 286, 385, 464, 494, 514, 551
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 385
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 531
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 258
BauI CACGAG 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 659
BbvI GCAGC 3 cut(s) 103, 201, 611
BccI CCATC 2 cut(s) 266, 334
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 2 cut(s) 284, 468
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 3 cut(s) 18, 89, 155
BfmI CTRYAG 1 cut(s) 7
BisI GCNGC 4 cut(s) 84, 117, 190, 625
BlsI GCNGC 4 cut(s) 85, 118, 191, 626
Bme1390I CCNGG 2 cut(s) 284, 468
Bme18I GGWCC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 8 cut(s) 254, 255, 286, 385, 464, 494, 514, 551
BmiI GGNNCC 3 cut(s) 160, 256, 515
BmrFI CCNGG 2 cut(s) 284, 468
BmrI ACTGGG 2 cut(s) 473, 544
BmsI GCATC 2 cut(s) 105, 476
BmuI ACTGGG 2 cut(s) 473, 544
BpiI GAAGAC 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 5 cut(s) 81, 162, 231, 369, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 370, 619
Bse1I ACTGG 2 cut(s) 479, 539
Bse3DI GCAATG 1 cut(s) 143
BseBI CCWGG 2 cut(s) 284, 468
BseDI CCNNGG 5 cut(s) 81, 162, 231, 369, 490
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 232, 370, 619
BseMI GCAATG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 479, 539
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 3 cut(s) 103, 201, 611
BseYI CCCAGC 2 cut(s) 461, 496
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 9 cut(s) 256, 282, 287, 344, 466, 495, 516, 531, 553
BshNI GGYRCC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 3 cut(s) 232, 370, 619
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 9 cut(s) 256, 282, 287, 344, 466, 495, 516, 531, 553
BsoBI CYCGRG 1 cut(s) 296
Bsp120I GGGCCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 3, 270
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 3 cut(s) 81, 83, 355
BspANI GGCC 9 cut(s) 256, 282, 287, 344, 466, 495, 516, 531, 553
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 160, 256, 515
BspPI GGATC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 479, 539
BssECI CCNNGG 5 cut(s) 81, 162, 231, 369, 490
BssMI GATC 2 cut(s) 3, 270
BssSI CACGAG 1 cut(s) 111
BssT1I CCWWGG 2 cut(s) 162, 369
Bst2BI CACGAG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 284, 468
Bst4CI ACNGT 2 cut(s) 232, 265
Bst6I CTCTTC 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 629
BstDSI CCRYGG 3 cut(s) 81, 231, 369
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 2 cut(s) 6, 273
BstMBI GATC 2 cut(s) 3, 270
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 2 cut(s) 284, 468
BstSCI CCNGG 2 cut(s) 282, 466
BstSFI CTRYAG 1 cut(s) 7
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 103, 201, 611
BstV2I GAAGAC 1 cut(s) 659
BsuRI GGCC 9 cut(s) 256, 282, 287, 344, 466, 495, 516, 531, 553
BtgI CCRYGG 3 cut(s) 81, 231, 369
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 1 cut(s) 629
Cfr13I GGNCC 8 cut(s) 254, 255, 286, 385, 464, 494, 514, 551
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 124
CviAII CATG 5 cut(s) 46, 133, 370, 614, 632
CviQI GTAC 1 cut(s) 124
DpnI GATC 2 cut(s) 5, 272
DpnII GATC 2 cut(s) 3, 270
EaeI YGGCCR 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 2 cut(s) 162, 369
Eco24I GRGCYC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 296
EcoO109I RGGNCCY 2 cut(s) 254, 286
EcoRII CCWGG 2 cut(s) 282, 466
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 162, 369
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 2 cut(s) 162, 369
FaeI CATG 5 cut(s) 49, 136, 373, 617, 635
FaqI GGGAC 1 cut(s) 289
FatI CATG 5 cut(s) 45, 132, 369, 613, 631
Fnu4HI GCNGC 4 cut(s) 84, 117, 190, 625
FokI GGATG 1 cut(s) 352
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 84, 117, 190, 625
FspBI CTAG 3 cut(s) 18, 89, 155
GluI GCNGC 4 cut(s) 84, 117, 190, 625
GsaI CCCAGC 2 cut(s) 465, 500
HaeIII GGCC 9 cut(s) 256, 282, 287, 344, 466, 495, 516, 531, 553
HgaI GACGC 1 cut(s) 318
Hin1II CATG 5 cut(s) 49, 136, 373, 617, 635
HincII GTYRAC 1 cut(s) 605
HindII GTYRAC 1 cut(s) 605
HinfI GANTC 1 cut(s) 655
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 124, 605
Hpy188I TCNGA 1 cut(s) 401
Hpy188III TCNNGA 7 cut(s) 111, 242, 268, 274, 298, 430, 523
Hpy8I GTNNAC 2 cut(s) 124, 605
Hpy99I CGWCG 1 cut(s) 334
HpyAV CCTTC 3 cut(s) 115, 355, 445
HpyCH4III ACNGT 2 cut(s) 232, 265
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 4 cut(s) 136, 222, 309, 627
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 5 cut(s) 49, 136, 373, 617, 635
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 2 cut(s) 3, 270
Lsp1109I GCAGC 3 cut(s) 103, 201, 611
LweI GCATC 2 cut(s) 105, 476
MaeI CTAG 3 cut(s) 18, 89, 155
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 439
MalI GATC 2 cut(s) 5, 272
MboI GATC 2 cut(s) 3, 270
MboII GAAGA 4 cut(s) 209, 631, 650, 664
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 8 cut(s) 15, 140, 209, 332, 398, 500, 530, 613
Mox20I TGGCCA 1 cut(s) 531
MscI TGGCCA 1 cut(s) 531
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 3 cut(s) 131, 419, 612
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 83, 446
MspR9I CCNGG 2 cut(s) 284, 468
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 2 cut(s) 284, 468
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 369
NdeII GATC 2 cut(s) 3, 270
NlaIII CATG 5 cut(s) 49, 136, 373, 617, 635
NlaIV GGNNCC 3 cut(s) 160, 256, 515
NmeAIII GCCGAG 1 cut(s) 471
PaeR7I CTCGAG 1 cut(s) 296
PctI GAATGC 1 cut(s) 414
PflMI CCANNNNNTGG 1 cut(s) 370
PkrI GCNGC 4 cut(s) 85, 118, 191, 626
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 282, 466
PspFI CCCAGC 2 cut(s) 461, 496
PspGI CCWGG 2 cut(s) 282, 466
PspN4I GGNNCC 3 cut(s) 160, 256, 515
PspOMI GGGCCC 1 cut(s) 254
PspPI GGNCC 8 cut(s) 254, 255, 286, 385, 464, 494, 514, 551
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 3 cut(s) 131, 419, 612
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 4 cut(s) 84, 117, 190, 625
Sau3AI GATC 2 cut(s) 3, 270
Sau96I GGNCC 8 cut(s) 254, 255, 286, 385, 464, 494, 514, 551
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 284, 468
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 2 cut(s) 105, 476
SfcI CTRYAG 1 cut(s) 7
Sfr274I CTCGAG 1 cut(s) 296
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 1 cut(s) 385
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 3 cut(s) 131, 419, 612
SmlI CTYRAG 2 cut(s) 170, 296
SmoI CTYRAG 2 cut(s) 170, 296
SpeI ACTAGT 1 cut(s) 88
SsiI CCGC 3 cut(s) 81, 83, 355
SspMI CTAG 3 cut(s) 18, 89, 155
StyD4I CCNGG 2 cut(s) 282, 466
StyI CCWWGG 2 cut(s) 162, 369
TaaI ACNGT 2 cut(s) 232, 265
TaiI ACGT 1 cut(s) 588
TaqI TCGA 5 cut(s) 297, 332, 348, 431, 642
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 86
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 539
TseI GCWGC 3 cut(s) 116, 189, 624
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 1 cut(s) 539
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 524
XhoI CTCGAG 1 cut(s) 296
XspI CTAG 3 cut(s) 18, 89, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.