Rh6AG031100

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009647
Physical Location & Seq
Forward (+)
3844021 .. 3845942
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG031100.1

Sequence Viewer

Length: 957 bp
ATGGCTCAAATCAACAGGCCAACGATGGAGGAAGATAAAGTTCATGCTTTCTCTTCCCCCATGCCATTAATGTCAGAACATTGGAAGACTGAAGAAGGGAAGAAATCCAATCCCTCCTGTACTTTGAGTGAGGTTTTGGGCTTGCGCGAGTTATTTTCTCCACAGGTGTGGAGAGCATCTCTAGCTGAGCTGGTTGGCACGGCAGTGCTTGTTTTTGCTATAGACGCAATTGTGATCTCCTCCATTGAGACCAATATCTCAGCACCAAACCTTGTATTGTCAATCCTGGTAGCAATCATCGTGACGATTCTCCTAATTGCTACCAACCCGGTTTCTGGAGGCCACATCAACCCCGTCATCACCTTTTCCACGGCACTGGTCGGCATCATTTCCTTCTCACGAGCTGCCGTGTACATTTTCATGCAATGCGTCGGAGGAGTTCTAGGTGCCCTGGCACTCAAGGCTGTTGTTAACAGCAGCCTTGAAGAAACATTCTCACTTGGAGGTTGCACCCTAACCGTGGTTTCTCCGGGCCGACAAGGGCCCATCACGGTCGGGATCGAGACAGGCCAGGCCCTTTGGCTCGAGATAATATGCACATTTATTTTCCTCTTTGCTTCGATATGGATGGCGTTCGACCACCGCCAAGCCCACGCCATGGGTAAGGTTATGGTCCTCTCTATCGTCGGAATAGTGGTGGGCATTCTTGTGTTCATCTCGACCTCTGTAACAACTGTGAAGGGCTACGCTGGGGCTGCAATGAACCCAGCTAGATGTCTCGGCCCAGCACTTGTTCGTGGGGGCCACCTCTGGACCGGCCACTGGGTATTTTGGGTCGGGCCTACTATTTCTTGCTTCGCTTTCTACTTGTACGTCAAGCTCATTCCCCGTCAACATTTCCACGCGGAGGGCTACAAGCACGATATCTTCAACTATGTGAAGACTCTTCCCAAGTAG

Protein Analysis

318

Amino Acids

34.37

Weight (kDa)

8.21

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 52 - 284 3.6e-39 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 658
AccII CGCG 2 cut(s) 147, 905
AciI CCGC 2 cut(s) 643, 905
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 817
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 3 cut(s) 121, 413, 872
AfiI CCNNNNNNNGG 5 cut(s) 335, 520, 658, 822, 907
AgsI TTSAA 2 cut(s) 485, 931
AjnI CCWGG 3 cut(s) 285, 450, 570
AleI CACNNNNGTG 2 cut(s) 166, 203
AluBI AGCT 5 cut(s) 185, 190, 404, 770, 880
AluI AGCT 5 cut(s) 185, 190, 404, 770, 880
Alw26I GTCTC 3 cut(s) 242, 557, 782
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 584
ApaI GGGCCC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 404, 477, 755
AseI ATTAAT 1 cut(s) 68
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 9 cut(s) 532, 542, 543, 574, 673, 782, 802, 813, 839
AsuC2I CCSGG 2 cut(s) 329, 531
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 673, 813
BaeGI GKGCMC 2 cut(s) 451, 546
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 546
BauI CACGAG 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 92, 947
BbvI GCAGC 3 cut(s) 391, 489, 742
BccI CCATC 3 cut(s) 19, 554, 622
BceAI ACGGC 3 cut(s) 216, 387, 392
BciT130I CCWGG 3 cut(s) 287, 452, 572
BcnI CCSGG 2 cut(s) 329, 531
BcoDI GTCTC 3 cut(s) 242, 557, 782
BfaI CTAG 3 cut(s) 182, 443, 771
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 405, 478, 756
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 3 cut(s) 406, 479, 757
Bme1390I CCNGG 5 cut(s) 287, 329, 452, 531, 572
Bme18I GGWCC 2 cut(s) 673, 813
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 9 cut(s) 532, 542, 543, 574, 673, 782, 802, 813, 839
BmiI GGNNCC 3 cut(s) 448, 544, 803
BmrFI CCNGG 5 cut(s) 287, 329, 452, 531, 572
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 2 cut(s) 185, 393
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 2 cut(s) 92, 947
BplI GAGNNNNNCTC 2 cut(s) 163, 195
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 2 cut(s) 329, 531
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 4 cut(s) 369, 450, 519, 657
Bsc4I CCNNNNNNNGG 5 cut(s) 335, 520, 658, 822, 907
Bse118I RCCGGY 1 cut(s) 815
Bse1I ACTGG 2 cut(s) 381, 827
Bse3DI GCAATG 2 cut(s) 431, 765
BseBI CCWGG 3 cut(s) 287, 452, 572
BseDI CCNNGG 4 cut(s) 369, 450, 519, 657
BseGI GGATG 1 cut(s) 633
BseLI CCNNNNNNNGG 5 cut(s) 335, 520, 658, 822, 907
BseMI GCAATG 2 cut(s) 431, 765
BseMII CTCAG 2 cut(s) 177, 273
BseNI ACTGG 2 cut(s) 381, 827
BseRI GAGGAG 2 cut(s) 229, 450
BseSI GKGCMC 2 cut(s) 451, 546
BseXI GCAGC 3 cut(s) 391, 489, 742
BseYI CCCAGC 3 cut(s) 749, 766, 784
Bsh1236I CGCG 2 cut(s) 147, 905
Bsh1285I CGRYCG 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 3 cut(s) 329, 530, 816
BslI CCNNNNNNNGG 5 cut(s) 335, 520, 658, 822, 907
BsmAI GTCTC 3 cut(s) 242, 557, 782
BsmI GAATGC 1 cut(s) 702
Bso31I GGTCTC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 584
Bsp120I GGGCCC 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 451, 546
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 234, 558
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 2 cut(s) 643, 905
BspCNI CTCAG 2 cut(s) 178, 272
BspFNI CGCG 2 cut(s) 147, 905
BspLI GGNNCC 3 cut(s) 448, 544, 803
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 431, 765
BsrFI RCCGGY 1 cut(s) 815
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 2 cut(s) 381, 827
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 4 cut(s) 369, 450, 519, 657
BssMI GATC 2 cut(s) 234, 558
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 3 cut(s) 287, 452, 572
Bst4CI ACNGT 3 cut(s) 520, 553, 736
Bst6I CTCTTC 2 cut(s) 58, 951
BstAUI TGTACA 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 2 cut(s) 186, 259
BstDSI CCRYGG 3 cut(s) 369, 519, 657
BstF5I GGATG 1 cut(s) 633
BstFNI CGCG 2 cut(s) 147, 905
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 237, 561
BstMAI GTCTC 3 cut(s) 242, 557, 782
BstMBI GATC 2 cut(s) 234, 558
BstMCI CGRYCG 1 cut(s) 555
BstMWI GCNNNNNNNGC 4 cut(s) 182, 224, 461, 755
BstNI CCWGG 3 cut(s) 287, 452, 572
BstSCI CCNGG 5 cut(s) 285, 327, 450, 529, 570
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 2 cut(s) 451, 546
BstUI CGCG 2 cut(s) 147, 905
BstV1I GCAGC 3 cut(s) 391, 489, 742
BstV2I GAAGAC 2 cut(s) 92, 947
BstXI CCANNNNNNTGG 2 cut(s) 168, 376
BtgI CCRYGG 3 cut(s) 369, 519, 657
BtsCI GGATG 1 cut(s) 633
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 3 cut(s) 210, 374, 820
Cac8I GCNNGC 1 cut(s) 143
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 9 cut(s) 532, 542, 543, 574, 673, 782, 802, 813, 839
CseI GACGC 2 cut(s) 233, 418
Csp6I GTAC 3 cut(s) 120, 412, 871
CviAII CATG 4 cut(s) 44, 61, 421, 658
CviQI GTAC 3 cut(s) 120, 412, 871
DdeI CTNAG 2 cut(s) 186, 259
DpnI GATC 2 cut(s) 236, 560
DpnII GATC 2 cut(s) 234, 558
EaeI YGGCCR 1 cut(s) 817
Eam1104I CTCTTC 2 cut(s) 58, 951
EarI CTCTTC 2 cut(s) 58, 951
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 1 cut(s) 546
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 2 cut(s) 673, 813
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 584
EcoO109I RGGNCCY 2 cut(s) 542, 574
EcoRII CCWGG 3 cut(s) 285, 450, 570
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 4 cut(s) 47, 64, 424, 661
FaiI YATR 9 cut(s) 45, 62, 221, 422, 595, 625, 659, 671, 936
FatI CATG 4 cut(s) 43, 60, 420, 657
Fnu4HI GCNGC 3 cut(s) 405, 478, 756
FokI GGATG 1 cut(s) 640
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 3 cut(s) 405, 478, 756
FspBI CTAG 3 cut(s) 182, 443, 771
GlaI GCGC 1 cut(s) 146
GluI GCNGC 3 cut(s) 405, 478, 756
GsaI CCCAGC 3 cut(s) 753, 770, 788
GsuI CTGGAG 1 cut(s) 357
HapII CCGG 3 cut(s) 329, 530, 816
HgaI GACGC 2 cut(s) 233, 418
HhaI GCGC 1 cut(s) 147
Hin1II CATG 4 cut(s) 47, 64, 424, 661
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 2 cut(s) 472, 893
HindII GTYRAC 2 cut(s) 472, 893
HinfI GANTC 2 cut(s) 307, 943
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 3 cut(s) 329, 530, 816
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 3 cut(s) 412, 472, 893
Hpy188I TCNGA 3 cut(s) 76, 434, 689
Hpy188III TCNNGA 8 cut(s) 301, 336, 399, 556, 562, 586, 718, 811
Hpy8I GTNNAC 3 cut(s) 412, 472, 893
Hpy99I CGWCG 2 cut(s) 434, 689
HpyAV CCTTC 3 cut(s) 89, 403, 733
HpyCH4III ACNGT 3 cut(s) 520, 553, 736
HpyCH4IV ACGT 1 cut(s) 873
HpyCH4V TGCA 4 cut(s) 424, 510, 597, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 224, 461, 755
HpyF3I CTNAG 2 cut(s) 186, 259
HpySE526I ACGT 1 cut(s) 873
Hsp92II CATG 4 cut(s) 47, 64, 424, 661
HspAI GCGC 1 cut(s) 145
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 2 cut(s) 234, 558
Lsp1109I GCAGC 3 cut(s) 391, 489, 742
LweI GCATC 2 cut(s) 185, 393
MaeI CTAG 3 cut(s) 182, 443, 771
MaeII ACGT 1 cut(s) 873
MaeIII GTNAC 2 cut(s) 301, 727
MalI GATC 2 cut(s) 236, 560
MboI GATC 2 cut(s) 234, 558
MboII GAAGA 9 cut(s) 44, 45, 97, 104, 112, 497, 919, 938, 952
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 451, 546
MluCI AATT 2 cut(s) 228, 315
MlyI GAGTC 1 cut(s) 937
MmeI TCCRAC 2 cut(s) 412, 667
MseI TTAA 2 cut(s) 68, 471
MslI CAYNNNNRTG 4 cut(s) 166, 203, 419, 707
MspI CCGG 3 cut(s) 329, 530, 816
MspR9I CCNGG 5 cut(s) 287, 329, 452, 531, 572
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 3 cut(s) 287, 452, 572
MvnI CGCG 2 cut(s) 147, 905
MwoI GCNNNNNNNGC 4 cut(s) 182, 224, 461, 755
NciI CCSGG 2 cut(s) 329, 531
NcoI CCATGG 1 cut(s) 657
NdeII GATC 2 cut(s) 234, 558
NlaIII CATG 4 cut(s) 47, 64, 424, 661
NlaIV GGNNCC 3 cut(s) 448, 544, 803
NmeAIII GCCGAG 1 cut(s) 759
NmuCI GTSAC 1 cut(s) 301
OliI CACNNNNGTG 2 cut(s) 166, 203
PaeR7I CTCGAG 1 cut(s) 584
PctI GAATGC 1 cut(s) 702
PfeI GAWTC 1 cut(s) 307
PflMI CCANNNNNTGG 1 cut(s) 658
PkrI GCNGC 3 cut(s) 406, 479, 757
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
PshBI ATTAAT 1 cut(s) 68
Psp6I CCWGG 3 cut(s) 285, 450, 570
PspFI CCCAGC 3 cut(s) 749, 766, 784
PspGI CCWGG 3 cut(s) 285, 450, 570
PspN4I GGNNCC 3 cut(s) 448, 544, 803
PspOMI GGGCCC 1 cut(s) 542
PspPI GGNCC 9 cut(s) 532, 542, 543, 574, 673, 782, 802, 813, 839
RsaI GTAC 3 cut(s) 121, 413, 872
RsaNI GTAC 3 cut(s) 120, 412, 871
RseI CAYNNNNRTG 4 cut(s) 166, 203, 419, 707
SaqAI TTAA 2 cut(s) 68, 471
SatI GCNGC 3 cut(s) 405, 478, 756
Sau3AI GATC 2 cut(s) 234, 558
Sau96I GGNCC 9 cut(s) 532, 542, 543, 574, 673, 782, 802, 813, 839
SchI GAGTC 1 cut(s) 937
ScrFI CCNGG 5 cut(s) 287, 329, 452, 531, 572
SduI GDGCHC 2 cut(s) 451, 546
SfaNI GCATC 2 cut(s) 185, 393
SfcI CTRYAG 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 584
SinI GGWCC 2 cut(s) 673, 813
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 4 cut(s) 166, 203, 419, 707
SmlI CTYRAG 2 cut(s) 458, 584
SmoI CTYRAG 2 cut(s) 458, 584
Sse9I AATT 2 cut(s) 228, 315
SsiI CCGC 2 cut(s) 643, 905
SspMI CTAG 3 cut(s) 182, 443, 771
StyD4I CCNGG 5 cut(s) 285, 327, 450, 529, 570
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 520, 553, 736
TaiI ACGT 1 cut(s) 876
TaqI TCGA 5 cut(s) 561, 585, 620, 636, 719
TasI AATT 2 cut(s) 228, 315
TatI WGTACW 2 cut(s) 119, 411
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 2 cut(s) 68, 471
Tru9I TTAA 2 cut(s) 68, 471
TscAI CASTG 3 cut(s) 210, 381, 827
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 3 cut(s) 404, 477, 755
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 4 cut(s) 32, 409, 703, 776
TspRI CASTG 3 cut(s) 210, 381, 827
Van91I CCANNNNNTGG 1 cut(s) 658
VpaK11BI GGWCC 2 cut(s) 673, 813
VspI ATTAAT 1 cut(s) 68
XhoI CTCGAG 1 cut(s) 584
XspI CTAG 3 cut(s) 182, 443, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.