FvH4_2g02400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007855
Physical Location & Seq
Forward (+)
1946249 .. 1949520
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02400.t4

Sequence Viewer

Length: 1209 bp
ATGGCTCTCCACACCCTCCTTCTGATCCTCTTAGCTCTCAACCACTTTCAATCTTCTACCTCTCTAATTTCCACCCCCGATCTGAATTCTCGCTACCCGAGTCCCAGAGCCATCTCGGATTTGAAGGAGGCGATAGTGAAAGGGATGGGATTCCAAGCCGATGATCTGAAGATATCGGGGTTTGATCTGAGGGAAGCGCAGGTGGGGCATTCGGTGGCGTATGAGTTTGACGTGGAGTACGTTGACCTCCCGTTGTTTCGGGTCGAGGATGAGAACGGGTTGGTCCAGAAGGAGAAGCTGGATCAAGGCTTGCCTCCGGTTTTGGCTCCGTTTCAGTTGGCCGGCCCGATGGAGCTGTGGATTCAGGACGGTAAAGACATGAGGCTATCGCTTCCGCATGATGTGGATGCTGGTGTGTTGAAGAAGGTGATTTTAGCAGAAGGGGCTGTGGTTACCGTGACGGGTGCTAGGTCAGTGAGCCTAAGGCACCCTCTTCAGCTCCCATTGCCATTGAATCGAACTAGCAGTGGTGTTGCTTCTGGGCTTGTTGCCCTAGCTGAATGGCTACGTCATGCTTCTAGAACCGAATCAGCTCCGTTGCTGTCTCTCCGTATTGTTGGTCCAACTGCTCTTACATCCCCTGCTTCAACCTCATCCTCCATGAACAACAAGCTCAAGCTTAAGCGGCTTGCTCCTGGCCTTGTGGAGTTGTCATCACCAGCTAACACAAGACCCATGTCTGTTGAGCTACAAAACCAAGAGACCACCATTTTAACTCCAAAATATTTCACAACTGTTTGGCCCCTTACTTCTGTTAATGGGTCGAATGGCAACTTGCTGGCTTTTGAGACACTGCTTTCGACTGTTCTTGGTCCCAAGGCAAACCAAAAAGGCTCCTTTAAATTGTTGAAGGCAGATGTGTCGGCGCAGACGTTTTTGAAGATTGGGTTTGGGGTGGAGAAGAAGGTGAAAGAAGGAAATGGGATAGACTGGACAGGTTTTCCAGCATGGAGGACAAAGCCTGAGACTGTAAGGATGCATTTTGAGGTGCTCGCCAAGGTTGATGGTGATAAAATTGTGCCAGAGAGAGTGGTTCCAGTTAACCCTGTCATCTTGGAGGATACTATCACACCTAATGTCCTGTCCGGGAATGTCTCCATGTCCAGGACCCCCGTAGCTCAACCTCCCCCTATTCCCTTCACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

43.59

Weight (kDa)

7.89

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 486
AciI CCGC 2 cut(s) 395, 685
AclWI GGATC 2 cut(s) 19, 309
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 2 cut(s) 188, 479
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 1164
AflII CTTAAG 1 cut(s) 680
AgsI TTSAA 7 cut(s) 50, 124, 421, 514, 648, 910, 940
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 694, 1163
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 5 cut(s) 609, 755, 842, 1019, 1159
AlwI GGATC 2 cut(s) 19, 309
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 4 cut(s) 339, 343, 697, 800
ApoI RAATTY 1 cut(s) 85
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
AspLEI GCGC 2 cut(s) 199, 928
AspS9I GGNCC 6 cut(s) 283, 344, 620, 801, 872, 1167
AsuC2I CCSGG 1 cut(s) 1147
AsuHPI GGTGA 5 cut(s) 439, 708, 979, 1079, 1192
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 4 cut(s) 283, 620, 872, 1167
AxyI CCTNAGG 1 cut(s) 482
BanI GGYRCC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 1053
BccI CCATC 4 cut(s) 119, 139, 343, 1058
BcgI CGANNNNNNTGC 2 cut(s) 903, 937
BciT130I CCWGG 2 cut(s) 696, 1165
BciVI GTATCC 1 cut(s) 1114
BcnI CCSGG 1 cut(s) 1147
BcoDI GTCTC 5 cut(s) 609, 755, 842, 1019, 1159
BfaI CTAG 4 cut(s) 468, 522, 554, 579
BfrI CTTAAG 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 190
BfuI GTATCC 1 cut(s) 1114
BisI GCNGC 1 cut(s) 686
BlsI GCNGC 1 cut(s) 687
Bme1390I CCNGG 3 cut(s) 696, 1147, 1165
Bme18I GGWCC 4 cut(s) 283, 620, 872, 1167
BmeT110I CYCGRG 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 6 cut(s) 283, 344, 620, 801, 872, 1167
BmiI GGNNCC 7 cut(s) 327, 488, 803, 874, 895, 1095, 1169
BmrFI CCNGG 3 cut(s) 696, 1147, 1165
BmsI GCATC 2 cut(s) 397, 1026
BoxI GACNNNNGTC 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 2 cut(s) 876, 1056
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 1164
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 995, 1097
Bse21I CCTNAGG 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 2 cut(s) 696, 1165
BseDI CCNNGG 2 cut(s) 876, 1056
BseGI GGATG 6 cut(s) 150, 274, 412, 635, 653, 1041
BseLI CCNNNNNNNGG 1 cut(s) 1164
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 179, 1014
BseNI ACTGG 2 cut(s) 995, 1097
BshFI GGCC 4 cut(s) 341, 345, 699, 802
BshNI GGYRCC 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 1053
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 3 cut(s) 317, 342, 1146
BslFI GGGAC 2 cut(s) 87, 858
BslI CCNNNNNNNGG 1 cut(s) 1164
BsmAI GTCTC 5 cut(s) 609, 755, 842, 1019, 1159
BsmFI GGGAC 2 cut(s) 87, 858
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 4 cut(s) 341, 345, 699, 802
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 1053
Bsp143I GATC 5 cut(s) 24, 79, 163, 184, 301
BspACI CCGC 2 cut(s) 395, 685
BspANI GGCC 4 cut(s) 341, 345, 699, 802
BspCNI CTCAG 2 cut(s) 180, 1015
BspLI GGNNCC 7 cut(s) 327, 488, 803, 874, 895, 1095, 1169
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 2 cut(s) 19, 309
BspT107I GGYRCC 1 cut(s) 486
BspTI CTTAAG 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 2 cut(s) 995, 1097
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 876, 1056
BssMI GATC 5 cut(s) 24, 79, 163, 184, 301
BssT1I CCWWGG 2 cut(s) 876, 1056
Bst2UI CCWGG 2 cut(s) 696, 1165
Bst4CI ACNGT 5 cut(s) 371, 457, 796, 865, 1030
Bst6I CTCTTC 1 cut(s) 498
BstAFI CTTAAG 1 cut(s) 680
BstC8I GCNNGC 5 cut(s) 311, 343, 690, 840, 1053
BstDEI CTNAG 4 cut(s) 31, 188, 482, 1023
BstEII GGTNACC 1 cut(s) 451
BstF5I GGATG 6 cut(s) 150, 274, 412, 635, 653, 1041
BstHHI GCGC 2 cut(s) 199, 928
BstKTI GATC 5 cut(s) 27, 82, 166, 187, 304
BstMAI GTCTC 5 cut(s) 609, 755, 842, 1019, 1159
BstMBI GATC 5 cut(s) 24, 79, 163, 184, 301
BstMWI GCNNNNNNNGC 4 cut(s) 205, 443, 505, 685
BstNI CCWGG 2 cut(s) 696, 1165
BstPAI GACNNNNGTC 1 cut(s) 736
BstPI GGTNACC 1 cut(s) 451
BstSCI CCNGG 3 cut(s) 694, 1145, 1163
Bsu36I CCTNAGG 1 cut(s) 482
BsuI GTATCC 1 cut(s) 1114
BsuRI GGCC 4 cut(s) 341, 345, 699, 802
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 6 cut(s) 150, 274, 412, 635, 653, 1041
BtsI GCAGTG 2 cut(s) 532, 851
BtsIMutI CAGTG 3 cut(s) 480, 532, 851
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 5 cut(s) 311, 343, 690, 840, 1053
CfoI GCGC 2 cut(s) 199, 928
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 6 cut(s) 283, 344, 620, 801, 872, 1167
Csp6I GTAC 1 cut(s) 238
CviAII CATG 7 cut(s) 379, 398, 572, 661, 736, 1008, 1159
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 4 cut(s) 31, 188, 482, 1023
DpnI GATC 5 cut(s) 26, 81, 165, 186, 303
DpnII GATC 5 cut(s) 24, 79, 163, 184, 301
DraI TTTAAA 1 cut(s) 901
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Eco130I CCWWGG 2 cut(s) 876, 1056
Eco31I GGTCTC 1 cut(s) 755
Eco32I GATATC 1 cut(s) 174
Eco47I GGWCC 4 cut(s) 283, 620, 872, 1167
Eco57I CTGAAG 2 cut(s) 188, 479
Eco81I CCTNAGG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 1167
EcoO65I GGTNACC 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 694, 1163
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 2 cut(s) 876, 1056
EcoT22I ATGCAT 1 cut(s) 1041
ErhI CCWWGG 2 cut(s) 876, 1056
FaeI CATG 7 cut(s) 382, 401, 575, 664, 739, 1011, 1162
FaiI YATR 8 cut(s) 222, 380, 399, 573, 662, 737, 1009, 1160
FaqI GGGAC 2 cut(s) 87, 858
FatI CATG 7 cut(s) 378, 397, 571, 660, 735, 1007, 1158
Fnu4HI GCNGC 1 cut(s) 686
FokI GGATG 6 cut(s) 157, 281, 419, 622, 640, 1048
FseI GGCCGGCC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 686
FspBI CTAG 4 cut(s) 468, 522, 554, 579
GlaI GCGC 2 cut(s) 198, 927
GluI GCNGC 1 cut(s) 686
HaeIII GGCC 4 cut(s) 341, 345, 699, 802
HapII CCGG 3 cut(s) 317, 342, 1146
HhaI GCGC 2 cut(s) 199, 928
Hin1II CATG 7 cut(s) 382, 401, 575, 664, 739, 1011, 1162
Hin6I GCGC 2 cut(s) 197, 926
HinP1I GCGC 2 cut(s) 197, 926
HincII GTYRAC 2 cut(s) 244, 1102
HindII GTYRAC 2 cut(s) 244, 1102
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 5 cut(s) 100, 150, 361, 514, 587
HpaI GTTAAC 1 cut(s) 1102
HpaII CCGG 3 cut(s) 317, 342, 1146
HphI GGTGA 5 cut(s) 439, 708, 979, 1079, 1192
Hpy166II GTNNAC 2 cut(s) 244, 1102
Hpy188I TCNGA 5 cut(s) 24, 84, 118, 168, 189
Hpy188III TCNNGA 3 cut(s) 286, 365, 579
Hpy8I GTNNAC 2 cut(s) 244, 1102
HpyAV CCTTC 9 cut(s) 29, 118, 283, 418, 434, 904, 958, 968, 1207
HpyCH4III ACNGT 5 cut(s) 371, 457, 796, 865, 1030
HpyCH4IV ACGT 4 cut(s) 231, 240, 568, 932
HpyCH4V TGCA 1 cut(s) 1039
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 443, 505, 685
HpyF3I CTNAG 4 cut(s) 31, 188, 482, 1023
HpySE526I ACGT 4 cut(s) 231, 240, 568, 932
Hsp92II CATG 7 cut(s) 382, 401, 575, 664, 739, 1011, 1162
HspAI GCGC 2 cut(s) 197, 926
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
KspAI GTTAAC 1 cut(s) 1102
Kzo9I GATC 5 cut(s) 24, 79, 163, 184, 301
LmnI GCTCC 6 cut(s) 331, 352, 504, 598, 697, 899
LweI GCATC 2 cut(s) 397, 1026
MaeI CTAG 4 cut(s) 468, 522, 554, 579
MaeII ACGT 4 cut(s) 231, 240, 568, 932
MaeIII GTNAC 2 cut(s) 451, 457
MalI GATC 5 cut(s) 26, 81, 165, 186, 303
MboI GATC 5 cut(s) 24, 79, 163, 184, 301
MboII GAAGA 6 cut(s) 45, 181, 433, 485, 952, 973
MhlI GDGCHC 1 cut(s) 1053
MluCI AATT 4 cut(s) 66, 85, 902, 1074
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 1041
MroNI GCCGGC 1 cut(s) 341
MseI TTAA 5 cut(s) 681, 773, 816, 900, 1101
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 3 cut(s) 317, 342, 1146
MspR9I CCNGG 3 cut(s) 696, 1147, 1165
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 2 cut(s) 696, 1165
MwoI GCNNNNNNNGC 4 cut(s) 205, 443, 505, 685
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 1147
NdeII GATC 5 cut(s) 24, 79, 163, 184, 301
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 7 cut(s) 382, 401, 575, 664, 739, 1011, 1162
NlaIV GGNNCC 7 cut(s) 327, 488, 803, 874, 895, 1095, 1169
NmuCI GTSAC 1 cut(s) 457
NsiI ATGCAT 1 cut(s) 1041
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 929
PctI GAATGC 1 cut(s) 208
PdiI GCCGGC 1 cut(s) 343
PfeI GAWTC 4 cut(s) 150, 361, 514, 587
PfoI TCCNGGA 2 cut(s) 1145, 1163
PkrI GCNGC 1 cut(s) 687
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
PpuMI RGGWCCY 1 cut(s) 1167
PshAI GACNNNNGTC 1 cut(s) 736
Psp5II RGGWCCY 1 cut(s) 1167
Psp6I CCWGG 2 cut(s) 694, 1163
PspEI GGTNACC 1 cut(s) 451
PspGI CCWGG 2 cut(s) 694, 1163
PspN4I GGNNCC 7 cut(s) 327, 488, 803, 874, 895, 1095, 1169
PspPI GGNCC 6 cut(s) 283, 344, 620, 801, 872, 1167
PspPPI RGGWCCY 1 cut(s) 1167
RigI GGCCGGCC 1 cut(s) 345
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 5 cut(s) 681, 773, 816, 900, 1101
SatI GCNGC 1 cut(s) 686
Sau3AI GATC 5 cut(s) 24, 79, 163, 184, 301
Sau96I GGNCC 6 cut(s) 283, 344, 620, 801, 872, 1167
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 3 cut(s) 696, 1147, 1165
SduI GDGCHC 1 cut(s) 1053
SfaNI GCATC 2 cut(s) 397, 1026
SinI GGWCC 4 cut(s) 283, 620, 872, 1167
SmlI CTYRAG 2 cut(s) 674, 680
SmoI CTYRAG 2 cut(s) 674, 680
Sse9I AATT 4 cut(s) 66, 85, 902, 1074
SsiI CCGC 2 cut(s) 395, 685
SspI AATATT 1 cut(s) 785
SspMI CTAG 4 cut(s) 468, 522, 554, 579
StyD4I CCNGG 3 cut(s) 694, 1145, 1163
StyI CCWWGG 2 cut(s) 876, 1056
TaaI ACNGT 5 cut(s) 371, 457, 796, 865, 1030
TaiI ACGT 4 cut(s) 234, 243, 571, 935
TaqI TCGA 4 cut(s) 264, 517, 824, 860
TasI AATT 4 cut(s) 66, 85, 902, 1074
TauI GCSGC 1 cut(s) 688
TfiI GAWTC 4 cut(s) 150, 361, 514, 587
Tru1I TTAA 5 cut(s) 681, 773, 816, 900, 1101
Tru9I TTAA 5 cut(s) 681, 773, 816, 900, 1101
TscAI CASTG 3 cut(s) 480, 532, 858
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 1 cut(s) 677
TspGWI ACGGA 3 cut(s) 318, 585, 599
TspRI CASTG 3 cut(s) 480, 532, 858
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 4 cut(s) 283, 620, 872, 1167
XapI RAATTY 1 cut(s) 85
XbaI TCTAGA 1 cut(s) 578
XspI CTAG 4 cut(s) 468, 522, 554, 579
Zsp2I ATGCAT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.