MD05G1112800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007855
Physical Location & Seq
Reverse (-)
23134302 .. 23136263
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1112800.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGTCTCCCAAAACGCTGCTTTTGATCCTCCTGACGGTGCAATTCCTCCCGTCCTCTCTCTCTCTGATTTCCGCGCCGGAGCTCCGCCCTCCCTACGCCACCCCCAGAGCCATCTCCGATTTGAAGGAGGCGATTGTTAAGGGATTAGGGTTTCAAGCGGAGGATCTCAAGGTGTCGGGGTTCGATTTGAGGGACGCGCAGGTGGGGCATTCGGTGGCGTACGAGTTCGACGTGGAGGTGGACAACAAGGTCCTGCCTTTCAAGCTTCTGGAGGACGTCGACCGCTGGGACTACGTCGACTTGCCTATTTTTAGGGTCGAGGACGAAAATGGGTTGGTCCAGAAAGGCAAATTGGGTGGTGGGTTGCCTCCGGTTTTGGCTCCGTTTCAGCTGGCTGGGCCCATGGAGCTCTGGATTCAGGACGCCAAAGATATGAGGATTTCGCTTCCTCATGATGTGGATGCTGGTGTGTTGAAGAAGGTGATATTGGCAGAAGGGGCTGTGGTCACAGTGAAGGGTGCCAGGTCTGTGAGTCTTCGCCACCCTCTTCAGCTCCCATTGCCATTGAACCGAACCAACAATGGGTTTGCTTCTGGTCTTGTGACCTTGGCTGAATGGCTGCGCCGTGCTTCTCGAACCCAATCATCTCCACTGCTGTCTCTCCGCATTGTTGGTCCGACTTCCCTCGCATCACCTTCCTCGACCTCTCCCTCCGTGAACAACAAGCTCAAACTAAAGCGCCTTGCCCCAGGCCTTGTGGAGTTGTCCTCACGATCCAACACCAAGCCCGTTTCTGTTGAGCTGCAAAACCAAGAGACCACCGCCATTTTAACCCCTAGCTACTTCAGCACGGTCTGGCCCCTTGCTTCCATCAATGGCTCGAACCCTAACTTGGTAGGTTTTGAGTCACTGCTTGCGTCTGTGCTGGGTCCTAAGGCCAATGAGAAAGGATCCTTCAAGTTATTGAAGGCAGATGTGTCTGCACAGACGTTTTTGAAGATCGGGTTTGGGGTCGAGAAGAAGCTCAAAGAAGGAGATGGGATAGATTTGAAGGGTTTTCCAGATTGGAGGACAAAGCCTGAGACCGTGAGGATGCATTTTGAGGTGCTGGCTAAGGTTGACGGGGATAAGATCATTCCAGAGAGAGTGGTTCCGGTTAACCCTGTACTAGTGGAGGACACCGTGGCACCGAGTGAGCTATTGGGCAATGTCTCCATGTCCACAGTCCCCATCATTCATCCTCCGGCGAGCCCCTATACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

45.63

Weight (kDa)

7.82

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 2 cut(s) 518, 1186
AccI GTMKAC 2 cut(s) 279, 297
AccII CGCG 2 cut(s) 74, 197
AciI CCGC 6 cut(s) 72, 85, 158, 283, 664, 822
AclWI GGATC 5 cut(s) 19, 171, 768, 945, 958
AcuI CTGAAG 2 cut(s) 533, 829
AcyI GRCGYC 2 cut(s) 276, 423
AdeI CACNNNGTG 1 cut(s) 1193
AfaI GTAC 2 cut(s) 221, 1167
AfiI CCNNNNNNNGG 3 cut(s) 34, 582, 892
AgsI TTSAA 9 cut(s) 124, 155, 262, 475, 568, 958, 967, 997, 1051
AhlI ACTAGT 1 cut(s) 1168
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 521, 748
AjuI GAANNNNNNNTTGG 6 cut(s) 335, 367, 470, 502, 875, 907
Alw21I GWGCWC 2 cut(s) 84, 411
Alw26I GTCTC 5 cut(s) 9, 663, 809, 1076, 1216
AlwI GGATC 5 cut(s) 19, 171, 768, 945, 958
AoxI GGCC 4 cut(s) 398, 751, 857, 936
ApaI GGGCCC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 16, 619, 802
AspLEI GCGC 4 cut(s) 76, 199, 624, 741
AspS9I GGNCC 7 cut(s) 250, 337, 398, 399, 674, 858, 929
AsuHPI GGTGA 2 cut(s) 493, 684
AvaII GGWCC 4 cut(s) 250, 337, 674, 929
AxyI CCTNAGG 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 402
BamHI GGATCC 1 cut(s) 950
BanI GGYRCC 2 cut(s) 518, 1186
BanII GRGCYC 4 cut(s) 84, 402, 411, 1253
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 84, 411
BbvI GCAGC 3 cut(s) 3, 606, 789
BccI CCATC 4 cut(s) 119, 878, 1031, 1238
BceAI ACGGC 1 cut(s) 609
BciT130I CCWGG 2 cut(s) 523, 750
BcoDI GTCTC 5 cut(s) 9, 663, 809, 1076, 1216
BcuI ACTAGT 1 cut(s) 1168
BfaI CTAG 2 cut(s) 837, 1169
BfoI RGCGCY 1 cut(s) 742
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 3 cut(s) 17, 620, 803
BlsI GCNGC 3 cut(s) 18, 621, 804
Bme1390I CCNGG 2 cut(s) 523, 750
Bme18I GGWCC 4 cut(s) 250, 337, 674, 929
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 7 cut(s) 250, 337, 398, 399, 674, 858, 929
BmiI GGNNCC 8 cut(s) 381, 400, 520, 860, 930, 952, 1152, 1188
BmrFI CCNGG 2 cut(s) 523, 750
BmsI GCATC 3 cut(s) 451, 698, 1083
BpiI GAAGAC 1 cut(s) 527
BplI GAGNNNNNCTC 2 cut(s) 752, 784
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 1 cut(s) 1113
BpuEI CTTGAG 1 cut(s) 152
BsaHI GRCGYC 2 cut(s) 276, 423
BsaI GGTCTC 2 cut(s) 809, 1076
BsaJI CCNNGG 4 cut(s) 402, 606, 748, 1182
BsaWI WCCGGW 2 cut(s) 370, 1153
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 582, 892
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 2 cut(s) 557, 1213
BseBI CCWGG 2 cut(s) 523, 750
BseDI CCNNGG 4 cut(s) 402, 606, 748, 1182
BseGI GGATG 3 cut(s) 466, 1098, 1237
BseLI CCNNNNNNNGG 3 cut(s) 34, 582, 892
BseMI GCAATG 2 cut(s) 557, 1213
BseMII CTCAG 1 cut(s) 1071
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 3 cut(s) 3, 606, 789
BseYI CCCAGC 3 cut(s) 285, 395, 925
BsgI GTGCAG 1 cut(s) 966
Bsh1236I CGCG 2 cut(s) 74, 197
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 4 cut(s) 400, 753, 859, 938
BshNI GGYRCC 2 cut(s) 518, 1186
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 2 cut(s) 84, 411
BsiSI CCGG 4 cut(s) 77, 371, 1154, 1244
BsiWI CGTACG 1 cut(s) 219
BslFI GGGAC 3 cut(s) 206, 302, 1211
BslI CCNNNNNNNGG 3 cut(s) 34, 582, 892
BsmAI GTCTC 5 cut(s) 9, 663, 809, 1076, 1216
BsmFI GGGAC 3 cut(s) 206, 302, 1211
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 4 cut(s) 400, 753, 859, 938
Bso31I GGTCTC 2 cut(s) 809, 1076
Bsp120I GGGCCC 1 cut(s) 398
Bsp1286I GDGCHC 4 cut(s) 84, 402, 411, 1253
Bsp143I GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 6 cut(s) 72, 85, 158, 283, 664, 822
BspANI GGCC 4 cut(s) 400, 753, 859, 938
BspCNI CTCAG 1 cut(s) 1072
BspFNI CGCG 2 cut(s) 74, 197
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 8 cut(s) 381, 400, 520, 860, 930, 952, 1152, 1188
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 5 cut(s) 19, 171, 768, 945, 958
BspT107I GGYRCC 2 cut(s) 518, 1186
BspTNI GGTCTC 2 cut(s) 809, 1076
BsrDI GCAATG 2 cut(s) 557, 1213
BssECI CCNNGG 4 cut(s) 402, 606, 748, 1182
BssMI GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
BssNI GRCGYC 2 cut(s) 276, 423
BssT1I CCWWGG 2 cut(s) 402, 606
Bst2UI CCWGG 2 cut(s) 523, 750
Bst4CI ACNGT 6 cut(s) 37, 511, 853, 1087, 1183, 1225
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 2 cut(s) 276, 423
BstC8I GCNNGC 4 cut(s) 393, 915, 1110, 1249
BstDEI CTNAG 3 cut(s) 933, 1080, 1113
BstDSI CCRYGG 2 cut(s) 402, 1182
BstF5I GGATG 3 cut(s) 466, 1098, 1237
BstFNI CGCG 2 cut(s) 74, 197
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 4 cut(s) 76, 199, 624, 741
BstKTI GATC 6 cut(s) 27, 166, 776, 953, 1002, 1134
BstMAI GTCTC 5 cut(s) 9, 663, 809, 1076, 1216
BstMBI GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 7 cut(s) 205, 262, 397, 406, 497, 559, 846
BstNI CCWGG 2 cut(s) 523, 750
BstSCI CCNGG 2 cut(s) 521, 748
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 2 cut(s) 74, 197
BstV1I GCAGC 3 cut(s) 3, 606, 789
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 2 cut(s) 163, 950
BstYI RGATCY 2 cut(s) 163, 950
Bsu36I CCTNAGG 1 cut(s) 933
BsuRI GGCC 4 cut(s) 400, 753, 859, 938
BtgI CCRYGG 2 cut(s) 402, 1182
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 466, 1098, 1237
BtsI GCAGTG 2 cut(s) 650, 908
BtsIMutI CAGTG 3 cut(s) 516, 650, 908
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 4 cut(s) 393, 915, 1110, 1249
CciI TCATGA 1 cut(s) 451
CfoI GCGC 4 cut(s) 76, 199, 624, 741
Cfr13I GGNCC 7 cut(s) 250, 337, 398, 399, 674, 858, 929
CseI GACGC 3 cut(s) 203, 431, 906
Csp6I GTAC 2 cut(s) 220, 1166
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 3 cut(s) 403, 452, 1216
CviQI GTAC 2 cut(s) 220, 1166
DdeI CTNAG 3 cut(s) 933, 1080, 1113
DpnI GATC 6 cut(s) 26, 165, 775, 952, 1001, 1133
DpnII GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
DraIII CACNNNGTG 1 cut(s) 1193
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 2 cut(s) 82, 409
Eco130I CCWWGG 2 cut(s) 402, 606
Eco147I AGGCCT 1 cut(s) 753
Eco24I GRGCYC 4 cut(s) 84, 402, 411, 1253
Eco31I GGTCTC 2 cut(s) 809, 1076
Eco47I GGWCC 4 cut(s) 250, 337, 674, 929
Eco53kI GAGCTC 2 cut(s) 82, 409
Eco57I CTGAAG 2 cut(s) 533, 829
Eco81I CCTNAGG 1 cut(s) 933
EcoICRI GAGCTC 2 cut(s) 82, 409
EcoO109I RGGNCCY 2 cut(s) 250, 929
EcoRII CCWGG 2 cut(s) 521, 748
EcoT14I CCWWGG 2 cut(s) 402, 606
EcoT22I ATGCAT 1 cut(s) 1098
EcoT38I GRGCYC 4 cut(s) 84, 402, 411, 1253
ErhI CCWWGG 2 cut(s) 402, 606
FaeI CATG 3 cut(s) 406, 455, 1219
FaiI YATR 5 cut(s) 404, 434, 453, 1217, 1257
FaqI GGGAC 3 cut(s) 206, 302, 1211
FatI CATG 3 cut(s) 402, 451, 1215
FblI GTMKAC 2 cut(s) 279, 297
Fnu4HI GCNGC 3 cut(s) 17, 620, 803
FokI GGATG 3 cut(s) 473, 1105, 1224
FriOI GRGCYC 4 cut(s) 84, 402, 411, 1253
Fsp4HI GCNGC 3 cut(s) 17, 620, 803
FspBI CTAG 2 cut(s) 837, 1169
GlaI GCGC 4 cut(s) 75, 198, 623, 740
GluI GCNGC 3 cut(s) 17, 620, 803
GsaI CCCAGC 3 cut(s) 289, 399, 929
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 4 cut(s) 400, 753, 859, 938
HapII CCGG 4 cut(s) 77, 371, 1154, 1244
HgaI GACGC 3 cut(s) 203, 431, 906
HhaI GCGC 4 cut(s) 76, 199, 624, 741
Hin1I GRCGYC 2 cut(s) 276, 423
Hin1II CATG 3 cut(s) 406, 455, 1219
Hin6I GCGC 4 cut(s) 74, 197, 622, 739
HinP1I GCGC 4 cut(s) 74, 197, 622, 739
HincII GTYRAC 4 cut(s) 280, 298, 1120, 1159
HindII GTYRAC 4 cut(s) 280, 298, 1120, 1159
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 415, 532, 905
HpaI GTTAAC 1 cut(s) 1159
HpaII CCGG 4 cut(s) 77, 371, 1154, 1244
HphI GGTGA 2 cut(s) 493, 684
Hpy166II GTNNAC 7 cut(s) 241, 280, 298, 718, 1120, 1159, 1221
Hpy188I TCNGA 3 cut(s) 66, 118, 678
Hpy8I GTNNAC 7 cut(s) 241, 280, 298, 718, 1120, 1159, 1221
Hpy99I CGWCG 3 cut(s) 233, 281, 299
HpyAV CCTTC 9 cut(s) 118, 472, 488, 508, 705, 961, 964, 1025, 1045
HpyCH4III ACNGT 6 cut(s) 37, 511, 853, 1087, 1183, 1225
HpyCH4IV ACGT 4 cut(s) 231, 276, 294, 989
HpyCH4V TGCA 4 cut(s) 40, 805, 983, 1096
HpyF10VI GCNNNNNNNGC 7 cut(s) 205, 262, 397, 406, 497, 559, 846
HpyF3I CTNAG 3 cut(s) 933, 1080, 1113
HpySE526I ACGT 4 cut(s) 231, 276, 294, 989
Hsp92I GRCGYC 2 cut(s) 276, 423
Hsp92II CATG 3 cut(s) 406, 455, 1219
HspAI GCGC 4 cut(s) 74, 197, 622, 739
KspAI GTTAAC 1 cut(s) 1159
Kzo9I GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
LmnI GCTCC 5 cut(s) 79, 87, 385, 406, 558
Lsp1109I GCAGC 3 cut(s) 3, 606, 789
LweI GCATC 3 cut(s) 451, 698, 1083
MaeI CTAG 2 cut(s) 837, 1169
MaeII ACGT 4 cut(s) 231, 276, 294, 989
MaeIII GTNAC 3 cut(s) 505, 601, 906
MalI GATC 6 cut(s) 26, 165, 775, 952, 1001, 1133
MboI GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
MboII GAAGA 5 cut(s) 487, 527, 539, 1009, 1030
MflI RGATCY 2 cut(s) 163, 950
MhlI GDGCHC 4 cut(s) 84, 402, 411, 1253
MluCI AATT 2 cut(s) 41, 350
MlyI GAGTC 2 cut(s) 541, 914
MmeI TCCRAC 2 cut(s) 701, 801
Mph1103I ATGCAT 1 cut(s) 1098
MseI TTAA 3 cut(s) 138, 830, 1158
MspA1I CMGCKG 2 cut(s) 285, 391
MspI CCGG 4 cut(s) 77, 371, 1154, 1244
MspR9I CCNGG 2 cut(s) 523, 750
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 2 cut(s) 523, 750
MvnI CGCG 2 cut(s) 74, 197
MwoI GCNNNNNNNGC 7 cut(s) 205, 262, 397, 406, 497, 559, 846
NcoI CCATGG 1 cut(s) 402
NdeII GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
NlaIII CATG 3 cut(s) 406, 455, 1219
NlaIV GGNNCC 8 cut(s) 381, 400, 520, 860, 930, 952, 1152, 1188
NmuCI GTSAC 3 cut(s) 505, 601, 906
NsiI ATGCAT 1 cut(s) 1098
PagI TCATGA 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 190
PceI AGGCCT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 415
Pfl23II CGTACG 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 18, 621, 804
PleI GAGTC 2 cut(s) 540, 913
PpsI GAGTC 2 cut(s) 540, 913
PpuMI RGGWCCY 2 cut(s) 250, 929
Psp124BI GAGCTC 2 cut(s) 84, 411
Psp5II RGGWCCY 2 cut(s) 250, 929
Psp6I CCWGG 2 cut(s) 521, 748
PspFI CCCAGC 3 cut(s) 285, 395, 925
PspGI CCWGG 2 cut(s) 521, 748
PspLI CGTACG 1 cut(s) 219
PspN4I GGNNCC 8 cut(s) 381, 400, 520, 860, 930, 952, 1152, 1188
PspOMI GGGCCC 1 cut(s) 398
PspPI GGNCC 7 cut(s) 250, 337, 398, 399, 674, 858, 929
PspPPI RGGWCCY 2 cut(s) 250, 929
PsuI RGATCY 2 cut(s) 163, 950
PsyI GACNNNGTC 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 2 cut(s) 221, 1167
RsaNI GTAC 2 cut(s) 220, 1166
SacI GAGCTC 2 cut(s) 84, 411
SalI GTCGAC 2 cut(s) 278, 296
SaqAI TTAA 3 cut(s) 138, 830, 1158
SatI GCNGC 3 cut(s) 17, 620, 803
Sau3AI GATC 6 cut(s) 24, 163, 773, 950, 999, 1131
Sau96I GGNCC 7 cut(s) 250, 337, 398, 399, 674, 858, 929
SchI GAGTC 2 cut(s) 541, 914
ScrFI CCNGG 2 cut(s) 523, 750
SduI GDGCHC 4 cut(s) 84, 402, 411, 1253
SfaNI GCATC 3 cut(s) 451, 698, 1083
SinI GGWCC 4 cut(s) 250, 337, 674, 929
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 1168
Sse9I AATT 2 cut(s) 41, 350
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 6 cut(s) 72, 85, 158, 283, 664, 822
SspMI CTAG 2 cut(s) 837, 1169
SstI GAGCTC 2 cut(s) 84, 411
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 2 cut(s) 521, 748
StyI CCWWGG 2 cut(s) 402, 606
TaaI ACNGT 6 cut(s) 37, 511, 853, 1087, 1183, 1225
TaiI ACGT 4 cut(s) 234, 279, 297, 992
TaqI TCGA 9 cut(s) 183, 228, 279, 297, 318, 634, 701, 881, 1014
TasI AATT 2 cut(s) 41, 350
TatI WGTACW 1 cut(s) 1165
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 3 cut(s) 138, 830, 1158
Tru9I TTAA 3 cut(s) 138, 830, 1158
TscAI CASTG 3 cut(s) 516, 657, 915
TseFI GTSAC 3 cut(s) 505, 601, 906
TseI GCWGC 3 cut(s) 16, 619, 802
Tsp45I GTSAC 3 cut(s) 505, 601, 906
TspDTI ATGAA 1 cut(s) 1226
TspGWI ACGGA 2 cut(s) 372, 703
TspRI CASTG 3 cut(s) 516, 657, 915
Tth111I GACNNNGTC 1 cut(s) 293
VpaK11BI GGWCC 4 cut(s) 250, 337, 674, 929
XmiI GTMKAC 2 cut(s) 279, 297
XspI CTAG 2 cut(s) 837, 1169
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.