MD02G1193100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007855
Physical Location & Seq
Reverse (-)
18315683 .. 18316567
885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1193100.v1.1.491

Sequence Viewer

Length: 297 bp
ATGGCTCTCAAACCGCTGTTTCTGATCCTCCTAACGGCACAATTCCTACCGTCTTCTCTCTCTCTGATTTCCGCGCCGGAGCTCCGCCCTCCCTACGCCACCCCCAGAGCCATCTATGATTTGATGGAGGCGATCTTGAAGGGATTAGGGTTTCAAGCGGAGGATCTAAAGGTGTCGGGGTTCGATGTGAGGGACGCGCAGGTGGGGCATTCAGTGGTGTACGAGTTTGACGTGGAGGTCGACAACAAGGTCCTATCTTTCAAGCTTCTGGAGGACGTCAACCGCCTGGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

10.91

Weight (kDa)

4.78

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 2 cut(s) 236, 248
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 74, 197
AciI CCGC 5 cut(s) 14, 72, 85, 158, 283
AclWI GGATC 2 cut(s) 19, 171
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 3 cut(s) 139, 155, 262
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 2 cut(s) 82, 265
AluI AGCT 2 cut(s) 82, 265
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 2 cut(s) 19, 171
AspLEI GCGC 2 cut(s) 76, 199
AspS9I GGNCC 1 cut(s) 250
AvaII GGWCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 84
BccI CCATC 2 cut(s) 118, 119
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 250
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 34
Bsh1236I CGCG 2 cut(s) 74, 197
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 24, 132, 163
BspACI CCGC 5 cut(s) 14, 72, 85, 158, 283
BspFNI CGCG 2 cut(s) 74, 197
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 2 cut(s) 19, 171
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 24, 132, 163
BssNI GRCGYC 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 276
BstFNI CGCG 2 cut(s) 74, 197
BstHHI GCGC 2 cut(s) 76, 199
BstKTI GATC 3 cut(s) 27, 135, 166
BstMBI GATC 3 cut(s) 24, 132, 163
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstUI CGCG 2 cut(s) 74, 197
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BtrI CACGTC 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 219
BveI ACCTGC 1 cut(s) 190
CfoI GCGC 2 cut(s) 76, 199
Cfr13I GGNCC 1 cut(s) 250
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 220
CviJI RGCY 4 cut(s) 5, 82, 110, 265
CviKI_1 RGCY 4 cut(s) 5, 82, 110, 265
CviQI GTAC 1 cut(s) 220
DpnI GATC 3 cut(s) 26, 134, 165
DpnII GATC 3 cut(s) 24, 132, 163
DrdI GACNNNNNNGTC 2 cut(s) 236, 248
DseDI GACNNNNNNGTC 2 cut(s) 236, 248
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 84
FaiI YATR 1 cut(s) 117
FaqI GGGAC 1 cut(s) 206
FblI GTMKAC 1 cut(s) 240
FriOI GRGCYC 1 cut(s) 84
GlaI GCGC 2 cut(s) 75, 198
GsuI CTGGAG 1 cut(s) 290
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 203
HhaI GCGC 2 cut(s) 76, 199
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 2 cut(s) 74, 197
HinP1I GCGC 2 cut(s) 74, 197
HincII GTYRAC 2 cut(s) 241, 280
HindII GTYRAC 2 cut(s) 241, 280
HindIII AAGCTT 1 cut(s) 263
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 220, 241, 280
Hpy188I TCNGA 2 cut(s) 24, 66
Hpy188III TCNNGA 2 cut(s) 136, 269
Hpy8I GTNNAC 3 cut(s) 220, 241, 280
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 2 cut(s) 231, 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpySE526I ACGT 2 cut(s) 231, 276
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 2 cut(s) 74, 197
Kzo9I GATC 3 cut(s) 24, 132, 163
LmnI GCTCC 2 cut(s) 79, 87
LpnPI CCDG 5 cut(s) 90, 118, 185, 254, 272
MaeII ACGT 2 cut(s) 231, 276
MalI GATC 3 cut(s) 26, 134, 165
MboI GATC 3 cut(s) 24, 132, 163
MboII GAAGA 1 cut(s) 45
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 1 cut(s) 41
MnlI CCTC 7 cut(s) 38, 99, 121, 154, 183, 229, 265
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 2 cut(s) 74, 197
MwoI GCNNNNNNNGC 1 cut(s) 205
NdeII GATC 3 cut(s) 24, 132, 163
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 237
PctI GAATGC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspPI GGNCC 1 cut(s) 250
PspPPI RGGWCCY 1 cut(s) 250
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 1 cut(s) 84
SalI GTCGAC 1 cut(s) 239
Sau3AI GATC 3 cut(s) 24, 132, 163
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 84
SetI ASST 8 cut(s) 84, 174, 204, 234, 240, 252, 267, 279
SinI GGWCC 1 cut(s) 250
Sse9I AATT 1 cut(s) 41
SsiI CCGC 5 cut(s) 14, 72, 85, 158, 283
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 2 cut(s) 234, 279
TaqI TCGA 2 cut(s) 183, 240
TasI AATT 1 cut(s) 41
TscAI CASTG 1 cut(s) 219
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 240
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.