FvH4_2g02480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007855
Physical Location & Seq
Forward (+)
1984679 .. 1988764
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02480.t1

Sequence Viewer

Length: 1290 bp
ATGGCTCTCCACACCCTCCTTCTGATCCTCTTAGCTCTCAACCACTTTCAATCTTCTACCTCTCTAATTTCCACCCCCGATCTGAATTCTCGCTACCCGAGTCCCAGAGCCATCTCGGATTTGAAGGAGGCGATAGTGAAAGGGATGGGATTCCAAGCCGATGATCTGAAGATATCGGGGTTTGATCTGAGGGAAGCGCAGGTGGGGCATTCGGTGGCGTATGAGTTTGACGTGGAGGTAGATAACAAGGTGCTGCCTTTTAAGCTTTTGGAGGATGTTGACCGCTGGGAGTACGTTGACCTCCCGTTGTTTCGGGTCGAGGATGAGAACGGGTTGGTCCAGAAGGAGAAGCTGGATCAAGGCTTGCCTCCGGTTTTGGCTCCGTTTCAGTTGGCCGGCCCGATGGAGCTGTGGATTCAGGACGGTAAAGACATGAGGCTATCGCTTCCGCATGATGTGGATGCTGGTGTGTTGAAGAAGGTGATTTTAGCAGAAGGGGCTGTGGTTACCGTGACGGGTGCTAGGTCAGTGAGCCTAAGGCACCCTCTTCAGCTCCCATTGCCATTGAATCGAACTAGCAGTGGTGTTGCTTCTGGGCTTGTTGCCCTAGCTGAATGGCTACGTCATGCTTCTAGAACCGAATCAGCTCCGTTGCTGTCTCTCCGTATTGTTGGTCCAACTGCTCTTACATCCCCTGCTTCAACCTCATCCTCCATGAACAACAAGCTCAAGCTTAAGCGGCTTGCTCCTGGCCTTGTGGAGTTGTCATCACCAGCTAACACAAGACCCATGTCTGTTGAGCTACAAAACCAAGAGACCACCATTTTAACTCCAAAATATTTCACAACTGTTTGGCCCCTTACTTCTGTTAATGGGTCGAATGGCAACTTGCTGGCTTTTGAGACACTGCTTTCGACTGTTCTTGGTCCCAAGGCAAACCAAAAAGGCTCCTTTAAATTGTTGAAGGCAGATGTGTCGGCGCAGACGTTTTTGAAGATTGGGTTTGGGGTGGAGAAGAAGGTGAAAGAAGGAAATGGGATAGACTGGACAGGTTTTCCAGCATGGAGGACAAAGCCTGAGACTGTAAGGATGCATTTTGAGGTGCTCGCCAAGGTTGATGGTGATAAAATTGTGCCAGAGAGAGTGGTTCCAGTTAACCCTGTCATCTTGGAGGATACTATCACACCTAATGTCCTGTCCGGGAATGTCTCCATGTCCAGGACCCCCGTAGCTCAACCTCCCCTATTCCCTTCACCTTGTAAATCGTCTTTTTTATGGGATTCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

46.78

Weight (kDa)

6.68

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 540
AciI CCGC 3 cut(s) 283, 449, 739
AclWI GGATC 2 cut(s) 19, 363
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 2 cut(s) 188, 533
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 1 cut(s) 1218
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 7 cut(s) 50, 124, 475, 568, 702, 964, 994
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 748, 1217
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 5 cut(s) 663, 809, 896, 1073, 1213
AlwI GGATC 2 cut(s) 19, 363
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 4 cut(s) 393, 397, 751, 854
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 85
ArsI GACNNNNNNTTYG 2 cut(s) 895, 927
AspLEI GCGC 2 cut(s) 199, 982
AspS9I GGNCC 6 cut(s) 337, 398, 674, 855, 926, 1221
AsuC2I CCSGG 1 cut(s) 1201
AsuHPI GGTGA 5 cut(s) 493, 762, 1033, 1133, 1245
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 4 cut(s) 337, 674, 926, 1221
AxyI CCTNAGG 1 cut(s) 536
BanI GGYRCC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 1 cut(s) 240
BccI CCATC 4 cut(s) 119, 139, 397, 1112
BcgI CGANNNNNNTGC 2 cut(s) 957, 991
BciT130I CCWGG 2 cut(s) 750, 1219
BciVI GTATCC 1 cut(s) 1168
BcnI CCSGG 1 cut(s) 1201
BcoDI GTCTC 5 cut(s) 663, 809, 896, 1073, 1213
BfaI CTAG 4 cut(s) 522, 576, 608, 633
BfrI CTTAAG 1 cut(s) 734
BfuAI ACCTGC 1 cut(s) 190
BfuI GTATCC 1 cut(s) 1168
BisI GCNGC 2 cut(s) 254, 740
BlsI GCNGC 2 cut(s) 255, 741
Bme1390I CCNGG 3 cut(s) 750, 1201, 1219
Bme18I GGWCC 4 cut(s) 337, 674, 926, 1221
BmeT110I CYCGRG 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 6 cut(s) 337, 398, 674, 855, 926, 1221
BmiI GGNNCC 7 cut(s) 381, 542, 857, 928, 949, 1149, 1223
BmrFI CCNGG 3 cut(s) 750, 1201, 1219
BmsI GCATC 2 cut(s) 451, 1080
BoxI GACNNNNGTC 1 cut(s) 790
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 1201
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 2 cut(s) 930, 1110
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 1218
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 1049, 1151
Bse21I CCTNAGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 750, 1219
BseDI CCNNGG 2 cut(s) 930, 1110
BseGI GGATG 7 cut(s) 150, 280, 328, 466, 689, 707, 1095
BseLI CCNNNNNNNGG 1 cut(s) 1218
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 2 cut(s) 179, 1068
BseNI ACTGG 2 cut(s) 1049, 1151
BseXI GCAGC 1 cut(s) 240
BseYI CCCAGC 1 cut(s) 285
BshFI GGCC 4 cut(s) 395, 399, 753, 856
BshNI GGYRCC 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 1107
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 3 cut(s) 371, 396, 1200
BslFI GGGAC 2 cut(s) 87, 912
BslI CCNNNNNNNGG 1 cut(s) 1218
BsmAI GTCTC 5 cut(s) 663, 809, 896, 1073, 1213
BsmFI GGGAC 2 cut(s) 87, 912
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 4 cut(s) 395, 399, 753, 856
Bso31I GGTCTC 1 cut(s) 809
BsoBI CYCGRG 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 1107
Bsp143I GATC 5 cut(s) 24, 79, 163, 184, 355
BspACI CCGC 3 cut(s) 283, 449, 739
BspANI GGCC 4 cut(s) 395, 399, 753, 856
BspCNI CTCAG 2 cut(s) 180, 1069
BspLI GGNNCC 7 cut(s) 381, 542, 857, 928, 949, 1149, 1223
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 2 cut(s) 19, 363
BspT107I GGYRCC 1 cut(s) 540
BspTI CTTAAG 1 cut(s) 734
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 2 cut(s) 1049, 1151
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 930, 1110
BssMI GATC 5 cut(s) 24, 79, 163, 184, 355
BssT1I CCWWGG 2 cut(s) 930, 1110
Bst2UI CCWGG 2 cut(s) 750, 1219
Bst4CI ACNGT 5 cut(s) 425, 511, 850, 919, 1084
Bst6I CTCTTC 1 cut(s) 552
BstAFI CTTAAG 1 cut(s) 734
BstC8I GCNNGC 5 cut(s) 365, 397, 744, 894, 1107
BstDEI CTNAG 4 cut(s) 31, 188, 536, 1077
BstEII GGTNACC 1 cut(s) 505
BstF5I GGATG 7 cut(s) 150, 280, 328, 466, 689, 707, 1095
BstHHI GCGC 2 cut(s) 199, 982
BstKTI GATC 5 cut(s) 27, 82, 166, 187, 358
BstMAI GTCTC 5 cut(s) 663, 809, 896, 1073, 1213
BstMBI GATC 5 cut(s) 24, 79, 163, 184, 355
BstMWI GCNNNNNNNGC 5 cut(s) 205, 262, 497, 559, 739
BstNI CCWGG 2 cut(s) 750, 1219
BstPAI GACNNNNGTC 1 cut(s) 790
BstPI GGTNACC 1 cut(s) 505
BstSCI CCNGG 3 cut(s) 748, 1199, 1217
BstV1I GCAGC 1 cut(s) 240
Bsu36I CCTNAGG 1 cut(s) 536
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 4 cut(s) 395, 399, 753, 856
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 7 cut(s) 150, 280, 328, 466, 689, 707, 1095
BtsI GCAGTG 2 cut(s) 586, 905
BtsIMutI CAGTG 3 cut(s) 534, 586, 905
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 5 cut(s) 365, 397, 744, 894, 1107
CfoI GCGC 2 cut(s) 199, 982
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 6 cut(s) 337, 398, 674, 855, 926, 1221
Csp6I GTAC 1 cut(s) 292
CviAII CATG 7 cut(s) 433, 452, 626, 715, 790, 1062, 1213
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 4 cut(s) 31, 188, 536, 1077
DpnI GATC 5 cut(s) 26, 81, 165, 186, 357
DpnII GATC 5 cut(s) 24, 79, 163, 184, 355
DraI TTTAAA 1 cut(s) 955
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 930, 1110
Eco31I GGTCTC 1 cut(s) 809
Eco32I GATATC 1 cut(s) 174
Eco47I GGWCC 4 cut(s) 337, 674, 926, 1221
Eco57I CTGAAG 2 cut(s) 188, 533
Eco81I CCTNAGG 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 1221
EcoO65I GGTNACC 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 748, 1217
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 2 cut(s) 930, 1110
EcoT22I ATGCAT 1 cut(s) 1095
ErhI CCWWGG 2 cut(s) 930, 1110
FaeI CATG 7 cut(s) 436, 455, 629, 718, 793, 1065, 1216
FaiI YATR 9 cut(s) 222, 434, 453, 627, 716, 791, 1063, 1214, 1276
FaqI GGGAC 2 cut(s) 87, 912
FatI CATG 7 cut(s) 432, 451, 625, 714, 789, 1061, 1212
Fnu4HI GCNGC 2 cut(s) 254, 740
FokI GGATG 7 cut(s) 157, 287, 335, 473, 676, 694, 1102
FseI GGCCGGCC 1 cut(s) 399
Fsp4HI GCNGC 2 cut(s) 254, 740
FspBI CTAG 4 cut(s) 522, 576, 608, 633
GlaI GCGC 2 cut(s) 198, 981
GluI GCNGC 2 cut(s) 254, 740
GsaI CCCAGC 1 cut(s) 289
HaeIII GGCC 4 cut(s) 395, 399, 753, 856
HapII CCGG 3 cut(s) 371, 396, 1200
HhaI GCGC 2 cut(s) 199, 982
Hin1II CATG 7 cut(s) 436, 455, 629, 718, 793, 1065, 1216
Hin6I GCGC 2 cut(s) 197, 980
HinP1I GCGC 2 cut(s) 197, 980
HincII GTYRAC 3 cut(s) 280, 298, 1156
HindII GTYRAC 3 cut(s) 280, 298, 1156
HindIII AAGCTT 2 cut(s) 263, 731
HinfI GANTC 6 cut(s) 100, 150, 415, 568, 641, 1280
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 3 cut(s) 371, 396, 1200
HphI GGTGA 5 cut(s) 493, 762, 1033, 1133, 1245
Hpy166II GTNNAC 3 cut(s) 280, 298, 1156
Hpy188I TCNGA 5 cut(s) 24, 84, 118, 168, 189
Hpy188III TCNNGA 4 cut(s) 340, 419, 633, 1284
Hpy8I GTNNAC 3 cut(s) 280, 298, 1156
HpyAV CCTTC 9 cut(s) 29, 118, 337, 472, 488, 958, 1012, 1022, 1260
HpyCH4III ACNGT 5 cut(s) 425, 511, 850, 919, 1084
HpyCH4IV ACGT 4 cut(s) 231, 294, 622, 986
HpyCH4V TGCA 1 cut(s) 1093
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 262, 497, 559, 739
HpyF3I CTNAG 4 cut(s) 31, 188, 536, 1077
HpySE526I ACGT 4 cut(s) 231, 294, 622, 986
Hsp92II CATG 7 cut(s) 436, 455, 629, 718, 793, 1065, 1216
HspAI GCGC 2 cut(s) 197, 980
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 24, 79, 163, 184, 355
LmnI GCTCC 6 cut(s) 385, 406, 558, 652, 751, 953
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 2 cut(s) 451, 1080
MaeI CTAG 4 cut(s) 522, 576, 608, 633
MaeII ACGT 4 cut(s) 231, 294, 622, 986
MaeIII GTNAC 2 cut(s) 505, 511
MalI GATC 5 cut(s) 26, 81, 165, 186, 357
MboI GATC 5 cut(s) 24, 79, 163, 184, 355
MboII GAAGA 6 cut(s) 45, 181, 487, 539, 1006, 1027
MhlI GDGCHC 1 cut(s) 1107
MluCI AATT 4 cut(s) 66, 85, 956, 1128
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 701
Mph1103I ATGCAT 1 cut(s) 1095
MroNI GCCGGC 1 cut(s) 395
MseI TTAA 6 cut(s) 261, 735, 827, 870, 954, 1155
MspA1I CMGCKG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 3 cut(s) 371, 396, 1200
MspR9I CCNGG 3 cut(s) 750, 1201, 1219
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 2 cut(s) 750, 1219
MwoI GCNNNNNNNGC 5 cut(s) 205, 262, 497, 559, 739
NaeI GCCGGC 1 cut(s) 397
NciI CCSGG 1 cut(s) 1201
NdeII GATC 5 cut(s) 24, 79, 163, 184, 355
NgoMIV GCCGGC 1 cut(s) 395
NlaIII CATG 7 cut(s) 436, 455, 629, 718, 793, 1065, 1216
NlaIV GGNNCC 7 cut(s) 381, 542, 857, 928, 949, 1149, 1223
NmuCI GTSAC 1 cut(s) 511
NsiI ATGCAT 1 cut(s) 1095
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 1 cut(s) 983
PctI GAATGC 1 cut(s) 208
PdiI GCCGGC 1 cut(s) 397
PfeI GAWTC 5 cut(s) 150, 415, 568, 641, 1280
PfoI TCCNGGA 2 cut(s) 1199, 1217
PkrI GCNGC 2 cut(s) 255, 741
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
PpuMI RGGWCCY 1 cut(s) 1221
PshAI GACNNNNGTC 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 748, 1217
PspEI GGTNACC 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 2 cut(s) 748, 1217
PspN4I GGNNCC 7 cut(s) 381, 542, 857, 928, 949, 1149, 1223
PspPI GGNCC 6 cut(s) 337, 398, 674, 855, 926, 1221
PspPPI RGGWCCY 1 cut(s) 1221
RigI GGCCGGCC 1 cut(s) 399
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SaqAI TTAA 6 cut(s) 261, 735, 827, 870, 954, 1155
SatI GCNGC 2 cut(s) 254, 740
Sau3AI GATC 5 cut(s) 24, 79, 163, 184, 355
Sau96I GGNCC 6 cut(s) 337, 398, 674, 855, 926, 1221
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 3 cut(s) 750, 1201, 1219
SduI GDGCHC 1 cut(s) 1107
SfaNI GCATC 2 cut(s) 451, 1080
SinI GGWCC 4 cut(s) 337, 674, 926, 1221
SmlI CTYRAG 2 cut(s) 728, 734
SmoI CTYRAG 2 cut(s) 728, 734
Sse9I AATT 4 cut(s) 66, 85, 956, 1128
SsiI CCGC 3 cut(s) 283, 449, 739
SspI AATATT 1 cut(s) 839
SspMI CTAG 4 cut(s) 522, 576, 608, 633
StyD4I CCNGG 3 cut(s) 748, 1199, 1217
StyI CCWWGG 2 cut(s) 930, 1110
TaaI ACNGT 5 cut(s) 425, 511, 850, 919, 1084
TaiI ACGT 4 cut(s) 234, 297, 625, 989
TaqI TCGA 4 cut(s) 318, 571, 878, 914
TasI AATT 4 cut(s) 66, 85, 956, 1128
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 5 cut(s) 150, 415, 568, 641, 1280
Tru1I TTAA 6 cut(s) 261, 735, 827, 870, 954, 1155
Tru9I TTAA 6 cut(s) 261, 735, 827, 870, 954, 1155
TscAI CASTG 3 cut(s) 534, 586, 912
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 1 cut(s) 731
TspGWI ACGGA 3 cut(s) 372, 639, 653
TspRI CASTG 3 cut(s) 534, 586, 912
Vha464I CTTAAG 1 cut(s) 734
VpaK11BI GGWCC 4 cut(s) 337, 674, 926, 1221
XapI RAATTY 1 cut(s) 85
XbaI TCTAGA 1 cut(s) 632
XspI CTAG 4 cut(s) 522, 576, 608, 633
Zsp2I ATGCAT 1 cut(s) 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.