Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0007855

Physical Location & Seq

Genomic Coordinates Reverse (-)

63787109 .. 63789478

Gene Structure 2370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015264

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGCAAATGGCTCTCCACACCCTGCTTTTGATCCTCTTAGCTCTTGCGCATTTTCAACCTTCTTCCTCTCTAATTTCGACCCCCGGTTGGAACTCTCGTTATCCCAGCCCCAGAGCCATCTCCGATTTGAAGGAGGCGATAGTGAAGGGATTGGGATTCCAAGCCGACGATCTGAAGATATCGGGATTTGATCTGAGGGACGCGCAGGTGGGGCATTCGGTGGCGTACGAGTTCGACGTGGAGGTAGATAAGAAGGTGCTTCCTTTCAAGCTTTTGGAGGACGTTGACCGCTGGGAGTACGTTGACCTGCCGGTATTTCGGGTCGAGGATGAAAGCGGGTTGGTCCCGAAAGGCAAATTGGACGACGGGTTGCCGCCCGTTTTGGCTCCGTTTCAGTTGGCCGGGCCCATGGAGCTGTGGATTCAGGACGCTAAAGACATGAGGCTCTCGCTCCCACATGATGTGGATGCTGGAGTGTTGAAGAAGGTGGTTTTAGCAGAAGGGGCTGTGGTTACTGTGACAGGTGCTAGGTCGGTGAGCCTAAGGCACCCTCTTCAGCTTCCATTGCCACTGAATCGGACCAGCAGCGGTTTTGCTTCTGGGCTCGTGACCCTAGCTGAATGGCTACGCCATGCTTCTAGAACCGAATCAGCTCCGTTGCTGTCTCTCCGCATTGTTGGTCCAACCGCTCTTACAGCCCCTGCCTCAACCTCTCCCTCCATGAACAACAAACTCAAGCTTAAGCGGCTTGCTCCTGGCCTTGTGGAGTTGTCATCACTGGCTAAAACAAGGCCCATGTCTGTTGAGCTACAAAACCAAGAGACCACCATTTTAACTCCAAAATACTTCACAACCGTTTGGCCCCTTGCTTCTGTCAATGGGTCGAACGCCAACTTGCTGGCTTTTGAGTCACTGCTTTCAACTGTTCTTGGTCCCAAGGCAAACAAAAAAGGTTCCTTTAAATTGTTGAAGGCAGATGTGTCAGCGCAGACGTTCTTGAAGATTGGGTTTGGGGTGGAGAAGAAGGTGAAAGAAGGTGATGGGATAGACTGGACAGGTTTTCCAGCATGGAGGACAAAGCCTGAGACTGTAAGGATGCATTTCGAGGTGCTTGCTAAGGTTGATGGCGATAAAATTGTGCCAGAGAGAGTGGTTCCAGTTAACCCTGCGATCTTGGAGGACACTATAGCACCTAATGTGCTGTCCGGGAATGTCTCTATGTCCACGACTCCCATAGTTCAATCTCCCCCTAATCCCTTAACCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

423

Amino Acids

45.8

Weight (kDa)

7.85

Isoelectric Point (pI)

36.24

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64510
fragaria_vesca	FvH4_2g02400 FvH4_2g02400 FvH4_2g02400 FvH4_2g02400 FvH4_2g02480 FvH4_2g02480
malus_domestica	MD02G1193100.v1.1 MD05G1112800.v1.1 MD10G1115400.v1.1 MD15G1348700.v1.1
prunus_persica	Prupe.8G157400_v2.0.a1
pyrus_communis	pycom10g09990
rosa_chinensis	RchiOBHm_Chr6g0246311
rosa_laevigata	RLG00000015264
rosa_multiflora	Rmu_sc0001066.1_g000010
rosa_roxburghii	Rroxscaffold_7G00214690
rosa_rugosa	Rorug05G0520200
rosa_samantha	Rh6AG034400 Rh6BG029500 Rh6CG029900 Rh6DG028800
rosa_wichuraiana	Rw6G002780 Rw6G002940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	196
Acc16I	TGCGCA	1 cut(s)	48
Acc36I	ACCTGC	2 cut(s)	196, 315
AccB1I	GGYRCC	1 cut(s)	546
AccBSI	CCGCTC	1 cut(s)	689
AccII	CGCG	1 cut(s)	203
AciI	CCGC	7 cut(s)	289, 336, 374, 588, 670, 687, 745
AclWI	GGATC	1 cut(s)	25
AcoI	YGGCCR	1 cut(s)	399
AcuI	CTGAAG	2 cut(s)	194, 539
AfaI	GTAC	2 cut(s)	227, 299
AfiI	CCNNNNNNNGG	1 cut(s)	87
AflII	CTTAAG	1 cut(s)	740
AgsI	TTSAA	8 cut(s)	56, 130, 268, 481, 921, 970, 1000, 1241
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	1 cut(s)	754
AjuI	GAANNNNNNNTTGG	2 cut(s)	341, 373
AluBI	AGCT	8 cut(s)	41, 271, 415, 559, 617, 653, 739, 808
AluI	AGCT	8 cut(s)	41, 271, 415, 559, 617, 653, 739, 808
Alw26I	GTCTC	4 cut(s)	669, 815, 1079, 1219
AlwI	GGATC	1 cut(s)	25
AlwNI	CAGNNNCTG	1 cut(s)	701
AoxI	GGCC	5 cut(s)	399, 404, 757, 791, 860
ApaI	GGGCCC	1 cut(s)	408
ApeKI	GCWGC	1 cut(s)	585
AspLEI	GCGC	3 cut(s)	49, 205, 988
AspS9I	GGNCC	8 cut(s)	343, 404, 405, 579, 680, 792, 861, 932
AsuC2I	CCSGG	3 cut(s)	84, 403, 1207
AsuHPI	GGTGA	3 cut(s)	547, 1039, 1049
AvaII	GGWCC	4 cut(s)	343, 579, 680, 932
AxyI	CCTNAGG	1 cut(s)	542
BaeGI	GKGCMC	1 cut(s)	408
BanI	GGYRCC	1 cut(s)	546
BanII	GRGCYC	2 cut(s)	408, 606
BauI	CACGAG	1 cut(s)	605
BbvI	GCAGC	1 cut(s)	597
BccI	CCATC	3 cut(s)	125, 1034, 1118
BcgI	CGANNNNNNTGC	2 cut(s)	1094, 1128
BciT130I	CCWGG	1 cut(s)	756
BcnI	CCSGG	3 cut(s)	84, 403, 1207
BcoDI	GTCTC	4 cut(s)	669, 815, 1079, 1219
BfaI	CTAG	3 cut(s)	528, 614, 639
BfmI	CTRYAG	1 cut(s)	1185
BfrI	CTTAAG	1 cut(s)	740
BfuAI	ACCTGC	2 cut(s)	196, 315
BisI	GCNGC	3 cut(s)	374, 586, 746
BlsI	GCNGC	3 cut(s)	375, 587, 747
Bme1390I	CCNGG	4 cut(s)	84, 403, 756, 1207
Bme18I	GGWCC	4 cut(s)	343, 579, 680, 932
BmgBI	CACGTC	1 cut(s)	238
BmgT120I	GGNCC	8 cut(s)	343, 404, 405, 579, 680, 792, 861, 932
BmiI	GGNNCC	8 cut(s)	345, 387, 406, 548, 863, 934, 955, 1155
BmrFI	CCNGG	4 cut(s)	84, 403, 756, 1207
BmsI	GCATC	2 cut(s)	457, 1086
BpmI	CTGGAG	1 cut(s)	492
Bpu10I	CCTNAGC	1 cut(s)	1116
BpuEI	CTTGAG	1 cut(s)	719
BpuMI	CCSGG	3 cut(s)	84, 403, 1207
BsaI	GGTCTC	1 cut(s)	815
BsaJI	CCNNGG	3 cut(s)	82, 408, 936
Bsc4I	CCNNNNNNNGG	1 cut(s)	87
Bse118I	RCCGGY	1 cut(s)	310
Bse1I	ACTGG	3 cut(s)	783, 1055, 1157
Bse21I	CCTNAGG	1 cut(s)	542
Bse3DI	GCAATG	1 cut(s)	563
BseBI	CCWGG	1 cut(s)	756
BseDI	CCNNGG	3 cut(s)	82, 408, 936
BseGI	GGATG	3 cut(s)	334, 472, 1101
BseLI	CCNNNNNNNGG	1 cut(s)	87
BseMI	GCAATG	1 cut(s)	563
BseMII	CTCAG	2 cut(s)	185, 1074
BseNI	ACTGG	3 cut(s)	783, 1055, 1157
BseSI	GKGCMC	1 cut(s)	408
BseXI	GCAGC	1 cut(s)	597
BseYI	CCCAGC	2 cut(s)	104, 291
Bsh1236I	CGCG	1 cut(s)	203
BshFI	GGCC	5 cut(s)	401, 406, 759, 793, 862
BshNI	GGYRCC	1 cut(s)	546
BsiSI	CCGG	4 cut(s)	84, 311, 402, 1206
BsiWI	CGTACG	1 cut(s)	225
BslFI	GGGAC	3 cut(s)	212, 329, 918
BslI	CCNNNNNNNGG	1 cut(s)	87
BsmAI	GTCTC	4 cut(s)	669, 815, 1079, 1219
BsmFI	GGGAC	3 cut(s)	212, 329, 918
BsmI	GAATGC	1 cut(s)	214
BsnI	GGCC	5 cut(s)	401, 406, 759, 793, 862
Bso31I	GGTCTC	1 cut(s)	815
Bsp120I	GGGCCC	1 cut(s)	404
Bsp1286I	GDGCHC	2 cut(s)	408, 606
Bsp143I	GATC	4 cut(s)	30, 169, 190, 1170
Bsp19I	CCATGG	1 cut(s)	408
BspACI	CCGC	7 cut(s)	289, 336, 374, 588, 670, 687, 745
BspANI	GGCC	5 cut(s)	401, 406, 759, 793, 862
BspCNI	CTCAG	2 cut(s)	186, 1075
BspFNI	CGCG	1 cut(s)	203
BspLI	GGNNCC	8 cut(s)	345, 387, 406, 548, 863, 934, 955, 1155
BspMI	ACCTGC	2 cut(s)	196, 315
BspPI	GGATC	1 cut(s)	25
BspT107I	GGYRCC	1 cut(s)	546
BspTI	CTTAAG	1 cut(s)	740
BspTNI	GGTCTC	1 cut(s)	815
BsrBI	CCGCTC	1 cut(s)	689
BsrDI	GCAATG	1 cut(s)	563
BsrFI	RCCGGY	1 cut(s)	310
BsrI	ACTGG	3 cut(s)	783, 1055, 1157
BssAI	RCCGGY	1 cut(s)	310
BssECI	CCNNGG	3 cut(s)	82, 408, 936
BssMI	GATC	4 cut(s)	30, 169, 190, 1170
BssSI	CACGAG	1 cut(s)	605
BssT1I	CCWWGG	2 cut(s)	408, 936
Bst2BI	CACGAG	1 cut(s)	605
Bst2UI	CCWGG	1 cut(s)	756
Bst4CI	ACNGT	4 cut(s)	517, 856, 925, 1090
Bst6I	CTCTTC	1 cut(s)	558
BstAFI	CTTAAG	1 cut(s)	740
BstC8I	GCNNGC	3 cut(s)	750, 900, 1113
BstDEI	CTNAG	5 cut(s)	37, 194, 542, 1083, 1116
BstDSI	CCRYGG	1 cut(s)	408
BstF5I	GGATG	3 cut(s)	334, 472, 1101
BstFNI	CGCG	1 cut(s)	203
BstHHI	GCGC	3 cut(s)	49, 205, 988
BstKTI	GATC	4 cut(s)	33, 172, 193, 1173
BstMAI	GTCTC	4 cut(s)	669, 815, 1079, 1219
BstMBI	GATC	4 cut(s)	30, 169, 190, 1170
BstMWI	GCNNNNNNNGC	6 cut(s)	211, 412, 503, 565, 695, 745
BstNI	CCWGG	1 cut(s)	756
BstSCI	CCNGG	4 cut(s)	82, 401, 754, 1205
BstSFI	CTRYAG	1 cut(s)	1185
BstSLI	GKGCMC	1 cut(s)	408
BstUI	CGCG	1 cut(s)	203
BstV1I	GCAGC	1 cut(s)	597
BstXI	CCANNNNNNTGG	1 cut(s)	898
Bsu36I	CCTNAGG	1 cut(s)	542
BsuRI	GGCC	5 cut(s)	401, 406, 759, 793, 862
BtgI	CCRYGG	1 cut(s)	408
BtrI	CACGTC	1 cut(s)	238
BtsCI	GGATG	3 cut(s)	334, 472, 1101
BtsI	GCAGTG	1 cut(s)	911
BtsIMutI	CAGTG	3 cut(s)	569, 776, 911
BveI	ACCTGC	2 cut(s)	196, 315
Cac8I	GCNNGC	3 cut(s)	750, 900, 1113
CaiI	CAGNNNCTG	1 cut(s)	701
CfoI	GCGC	3 cut(s)	49, 205, 988
Cfr10I	RCCGGY	1 cut(s)	310
Cfr13I	GGNCC	8 cut(s)	343, 404, 405, 579, 680, 792, 861, 932
CseI	GACGC	2 cut(s)	209, 437
Csp6I	GTAC	2 cut(s)	226, 298
CviAII	CATG	7 cut(s)	409, 439, 458, 632, 721, 796, 1068
CviQI	GTAC	2 cut(s)	226, 298
DdeI	CTNAG	5 cut(s)	37, 194, 542, 1083, 1116
DpnI	GATC	4 cut(s)	32, 171, 192, 1172
DpnII	GATC	4 cut(s)	30, 169, 190, 1170
DraI	TTTAAA	1 cut(s)	961
EaeI	YGGCCR	1 cut(s)	399
Eam1104I	CTCTTC	1 cut(s)	558
EarI	CTCTTC	1 cut(s)	558
Eco130I	CCWWGG	2 cut(s)	408, 936
Eco24I	GRGCYC	2 cut(s)	408, 606
Eco31I	GGTCTC	1 cut(s)	815
Eco32I	GATATC	1 cut(s)	180
Eco47I	GGWCC	4 cut(s)	343, 579, 680, 932
Eco57I	CTGAAG	2 cut(s)	194, 539
Eco81I	CCTNAGG	1 cut(s)	542
EcoRII	CCWGG	1 cut(s)	754
EcoRV	GATATC	1 cut(s)	180
EcoT14I	CCWWGG	2 cut(s)	408, 936
EcoT22I	ATGCAT	1 cut(s)	1101
EcoT38I	GRGCYC	2 cut(s)	408, 606
ErhI	CCWWGG	2 cut(s)	408, 936
FaeI	CATG	7 cut(s)	412, 442, 461, 635, 724, 799, 1071
FaqI	GGGAC	3 cut(s)	212, 329, 918
FatI	CATG	7 cut(s)	408, 438, 457, 631, 720, 795, 1067
FauI	CCCGC	1 cut(s)	329
Fnu4HI	GCNGC	3 cut(s)	374, 586, 746
FokI	GGATG	3 cut(s)	341, 479, 1108
FriOI	GRGCYC	2 cut(s)	408, 606
Fsp4HI	GCNGC	3 cut(s)	374, 586, 746
FspBI	CTAG	3 cut(s)	528, 614, 639
FspI	TGCGCA	1 cut(s)	48
GlaI	GCGC	3 cut(s)	48, 204, 987
GluI	GCNGC	3 cut(s)	374, 586, 746
GsaI	CCCAGC	2 cut(s)	108, 295
GsuI	CTGGAG	1 cut(s)	492
HaeIII	GGCC	5 cut(s)	401, 406, 759, 793, 862
HapII	CCGG	4 cut(s)	84, 311, 402, 1206
HgaI	GACGC	2 cut(s)	209, 437
HhaI	GCGC	3 cut(s)	49, 205, 988
Hin1II	CATG	7 cut(s)	412, 442, 461, 635, 724, 799, 1071
Hin6I	GCGC	3 cut(s)	47, 203, 986
HinP1I	GCGC	3 cut(s)	47, 203, 986
HincII	GTYRAC	3 cut(s)	286, 304, 1162
HindII	GTYRAC	3 cut(s)	286, 304, 1162
HindIII	AAGCTT	2 cut(s)	269, 737
HinfI	GANTC	6 cut(s)	156, 421, 574, 647, 908, 1228
HpaI	GTTAAC	1 cut(s)	1162
HpaII	CCGG	4 cut(s)	84, 311, 402, 1206
HphI	GGTGA	3 cut(s)	547, 1039, 1049
Hpy166II	GTNNAC	4 cut(s)	286, 304, 1162, 1224
Hpy188I	TCNGA	4 cut(s)	124, 174, 195, 579
Hpy188III	TCNNGA	6 cut(s)	183, 346, 425, 607, 639, 997
Hpy8I	GTNNAC	4 cut(s)	286, 304, 1162, 1224
Hpy99I	CGWCG	3 cut(s)	170, 239, 368
HpyAV	CCTTC	9 cut(s)	69, 124, 139, 247, 478, 494, 964, 1018, 1028
HpyCH4III	ACNGT	4 cut(s)	517, 856, 925, 1090
HpyCH4IV	ACGT	4 cut(s)	237, 282, 300, 992
HpyCH4V	TGCA	2 cut(s)	4, 1099
HpyF10VI	GCNNNNNNNGC	6 cut(s)	211, 412, 503, 565, 695, 745
HpyF3I	CTNAG	5 cut(s)	37, 194, 542, 1083, 1116
HpySE526I	ACGT	4 cut(s)	237, 282, 300, 992
Hsp92II	CATG	7 cut(s)	412, 442, 461, 635, 724, 799, 1071
HspAI	GCGC	3 cut(s)	47, 203, 986
KspAI	GTTAAC	1 cut(s)	1162
Kzo9I	GATC	4 cut(s)	30, 169, 190, 1170
LmnI	GCTCC	5 cut(s)	391, 412, 456, 658, 757
Lsp1109I	GCAGC	1 cut(s)	597
LweI	GCATC	2 cut(s)	457, 1086
MaeI	CTAG	3 cut(s)	528, 614, 639
MaeII	ACGT	4 cut(s)	237, 282, 300, 992
MaeIII	GTNAC	4 cut(s)	511, 517, 607, 909
MalI	GATC	4 cut(s)	32, 171, 192, 1172
MbiI	CCGCTC	1 cut(s)	689
MboI	GATC	4 cut(s)	30, 169, 190, 1170
MboII	GAAGA	6 cut(s)	54, 187, 493, 545, 1012, 1033
MhlI	GDGCHC	2 cut(s)	408, 606
MluCI	AATT	4 cut(s)	72, 356, 962, 1134
MlyI	GAGTC	2 cut(s)	917, 1222
MmeI	TCCRAC	2 cut(s)	68, 707
Mph1103I	ATGCAT	1 cut(s)	1101
MseI	TTAA	5 cut(s)	741, 833, 960, 1161, 1259
MspA1I	CMGCKG	2 cut(s)	291, 588
MspCI	CTTAAG	1 cut(s)	740
MspI	CCGG	4 cut(s)	84, 311, 402, 1206
MspR9I	CCNGG	4 cut(s)	84, 403, 756, 1207
Mva1269I	GAATGC	1 cut(s)	214
MvaI	CCWGG	1 cut(s)	756
MvnI	CGCG	1 cut(s)	203
MwoI	GCNNNNNNNGC	6 cut(s)	211, 412, 503, 565, 695, 745
NciI	CCSGG	3 cut(s)	84, 403, 1207
NcoI	CCATGG	1 cut(s)	408
NdeII	GATC	4 cut(s)	30, 169, 190, 1170
NlaIII	CATG	7 cut(s)	412, 442, 461, 635, 724, 799, 1071
NlaIV	GGNNCC	8 cut(s)	345, 387, 406, 548, 863, 934, 955, 1155
NmuCI	GTSAC	3 cut(s)	517, 607, 909
NsbI	TGCGCA	1 cut(s)	48
NsiI	ATGCAT	1 cut(s)	1101
PaqCI	CACCTGC	1 cut(s)	196
PcsI	WCGNNNNNNNCGW	1 cut(s)	234
PctI	GAATGC	1 cut(s)	214
PfeI	GAWTC	4 cut(s)	156, 421, 574, 647
Pfl23II	CGTACG	1 cut(s)	225
PfoI	TCCNGGA	1 cut(s)	1205
PkrI	GCNGC	3 cut(s)	375, 587, 747
PleI	GAGTC	2 cut(s)	916, 1222
PpsI	GAGTC	2 cut(s)	916, 1222
Psp6I	CCWGG	1 cut(s)	754
PspFI	CCCAGC	2 cut(s)	104, 291
PspGI	CCWGG	1 cut(s)	754
PspLI	CGTACG	1 cut(s)	225
PspN4I	GGNNCC	8 cut(s)	345, 387, 406, 548, 863, 934, 955, 1155
PspOMI	GGGCCC	1 cut(s)	404
PspPI	GGNCC	8 cut(s)	343, 404, 405, 579, 680, 792, 861, 932
PstNI	CAGNNNCTG	1 cut(s)	701
RsaI	GTAC	2 cut(s)	227, 299
RsaNI	GTAC	2 cut(s)	226, 298
SaqAI	TTAA	5 cut(s)	741, 833, 960, 1161, 1259
SatI	GCNGC	3 cut(s)	374, 586, 746
Sau3AI	GATC	4 cut(s)	30, 169, 190, 1170
Sau96I	GGNCC	8 cut(s)	343, 404, 405, 579, 680, 792, 861, 932
SchI	GAGTC	2 cut(s)	917, 1222
ScrFI	CCNGG	4 cut(s)	84, 403, 756, 1207
SduI	GDGCHC	2 cut(s)	408, 606
SfaNI	GCATC	2 cut(s)	457, 1086
SfcI	CTRYAG	1 cut(s)	1185
SinI	GGWCC	4 cut(s)	343, 579, 680, 932
SmlI	CTYRAG	2 cut(s)	734, 740
SmoI	CTYRAG	2 cut(s)	734, 740
Sse9I	AATT	4 cut(s)	72, 356, 962, 1134
SsiI	CCGC	7 cut(s)	289, 336, 374, 588, 670, 687, 745
SspMI	CTAG	3 cut(s)	528, 614, 639
StyD4I	CCNGG	4 cut(s)	82, 401, 754, 1205
StyI	CCWWGG	2 cut(s)	408, 936
TaaI	ACNGT	4 cut(s)	517, 856, 925, 1090
TaiI	ACGT	4 cut(s)	240, 285, 303, 995
TaqI	TCGA	5 cut(s)	77, 234, 324, 884, 1104
TasI	AATT	4 cut(s)	72, 356, 962, 1134
TauI	GCSGC	2 cut(s)	376, 748
TfiI	GAWTC	4 cut(s)	156, 421, 574, 647
Tru1I	TTAA	5 cut(s)	741, 833, 960, 1161, 1259
Tru9I	TTAA	5 cut(s)	741, 833, 960, 1161, 1259
TscAI	CASTG	3 cut(s)	576, 783, 918
TseFI	GTSAC	3 cut(s)	517, 607, 909
TseI	GCWGC	1 cut(s)	585
Tsp45I	GTSAC	3 cut(s)	517, 607, 909
TspDTI	ATGAA	2 cut(s)	345, 737
TspGWI	ACGGA	2 cut(s)	378, 645
TspRI	CASTG	3 cut(s)	576, 783, 918
Vha464I	CTTAAG	1 cut(s)	740
VpaK11BI	GGWCC	4 cut(s)	343, 579, 680, 932
XbaI	TCTAGA	1 cut(s)	638
XspI	CTAG	3 cut(s)	528, 614, 639
Zsp2I	ATGCAT	1 cut(s)	1101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.