FvH4_2g02540

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011518
Physical Location & Seq
Forward (+)
2024803 .. 2028479
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02540.t2

Sequence Viewer

Length: 2940 bp
ATGTCGTTCCTCCGGCAACGGCAACTCCCTCCGGTTCTCCTTCTCTCTCTCCTCTTCCTCTCCTCACTCTCTCCCTCCACATCATACGAGCTCGACCAACTCTTGAAGATCAAAGCCTCACTTGAAACTTCAAACCCAACTCTGTTCAGTTCATGGAACCAAGAAACTCCCACGTGCAACTTCACTGGGATTGTCTGCAACTCAAATGAGCTCGTCTCAGAAATCAATCTTTCTCAGCAGAACCTCTCTGGCTCTCTTCCTTTCGACGCATTATGTTCCCTTCCTGAACTCAAAAAGCTCTCTTTTGGTTCAAATTTTCTACACGGAAGCCTAACAAAGGATTTGAAAAAGTGTACTAATTTGCAGGAACTTGATTTGGGTAAGAATTCTTTCACTGGAGAAGTGCCTGAGTTGGGTTCTTTGAGTCAGTTGAGGCTGCTGAGTCTGAATGCTAGTGGGGTTTCAGGGCTTTTTCCATGGAAGTCACTTGAGAATCTGACGGAGTTGGCTTTTTTGAGCCTTGGTGATAATGTATTCGATCAAAGTCCGATTACAACGGAGTTGTCAAAGCTTGACAAGCTTTACTGGCTTTACCTCTCAAACTGTAGCATCACAGGTGAAATCCCAAATGGTATTGGAAACCTGACTCTGCTTAAGAATCTGGAGCTTGCTGATAACCAATTGGTTGGTGAAATTCCTCAGAGTATTATAAATCTCAACAAGCTTTGGCAGCTTGAGCTTTATGGTAATTCTCTGAGTGGGAAAATCCCAGTTGGGTTTGGCAACCTCATAAGCCTCAGGAACTTTGATGCTTCAAGTAACAAGCTTGAGGGTGATCTCTCTGAGCTCAAGTCCTTGACAGGGCTTGTGTCTCTGCACCTGTTTGGGAATCAATTAGTCGGCGAAGTCCCGGAAGAGTTTGGAGAGTTCAAGAACCTTGTTGAGCTCTCCCTTTACACCAACAAGCTCACTGGCTCTCTTCCTCAGAAGCTTGGTTCTTGGTCTGGCATGGAGTACATTGATGTTTCAGAGAACTATTTGACTGGTCCTATACCCCCTGGGATGTGCAAGAATGATAGAATGATTGATATTCTTCTGCTGCAGAACAATTTCACAGGTGGGATTCCTGAATCCTATGCAGATTGCAAGTCTTTGGTCCGGTTACGTGTGAATAACAATTCACTTTCGGGTACTGTCCCTGCTGGGATTTGGAGCTTGCCAAATGTTACTATTCTTGATCTCTCCATGAATCAATTTGAAGGTCCCCTCGCTTCAGATGTTGGTAAGGCTAATTCTCTGTCATTATTACTGTTAAATAATAATCGATTTTCGGGTGAACTGCCTGCTGCTTTGTCTGAAGCTACATCATTGGTTACAATTCAGCTTAGTGAGAACCAATTTGAGGGTCAAATTCCATTAACAATTGGGAACTTGAAGAAATTAGGTAGTCTTCATTTAGAGCATAATATGTTGTCTGGTGCCATACCGGACTCACTAGGCTCATGTGTTAGGATTTCTGAAATCAACCTTGCCCATAACATTCTCTCTGGCCAAATCCCACCAAGTCTTGGCTCATTGCCTAACTTGAACTCCTTGAACTTATCTGGAAACCAGCTTTCTAGTGAAGTTCCTGCAACTTTGTCATCTCTCAAACTCAGCCTTCTTGATCTTACAAACAACCAGTTGTCTGGCCGTGTGCCCCAGTCACTATCCATTGCAGCTTTTAATGAAAGCTTTGCTGGAAATCCTGGTTTGTGCAGCCAAACCATGAAGAATTTGCGGCCTTGTTCTTCAAATTCGAACTCTCCTAGTCACCCAAGGATATTTTTATCTTCTTTTATTGCTGGAGTACTTCTTCTGCTTATTTTGCTTGGTGTGTTCTTGCTATGGAAGCTAAGGAGGAGTGATCTTGATCAACCATTGAAGAGTAATTCATGGACTATGAAGCAGTACCATGTGTTAAGCTTCACAGAGAATGAAATCCTAGAGTCAATCAAAGCTGAAAACTTGATTGGCAAAGGAGGGTCAGGAAATGTGTACAAAGTACAACTGAGAGATGGGAAAGCACTTGCTGTGAAGCACATCTGGACCCCAGCAGACTCCGCTAACCAGAAAACTTATCGAAGCACTACTTCCATTTTGAAGAAGCCTAAAACAAGCTCATCGGAGTATGATGCCGAGGTGGCCACACTCAGCTCATTGAGGCATGTGAATGTGGTGAAGCTATACTGCAGCATAAGTAGTGAGGACAGCAAGCTGCTGGTTTACGAGTACTTTCCTAATGGGAGCTTATGGGATCAGCTGCACATGAATAAGAAGGCCAAGATGGGGTGGGAAGTGAGGTATGAGATTGCTTTTGGGGCTGCAAGAGGATTGGAGTATCTCCACCATGGTAGTCACAGACCTGTTATACATAGAGATGTGAAGTCTAGTAATATTCTGCTAGATGGAGATTGGAAGCCTAGAATTGCTGATTTTGGGCTTGCCAAGATTGTGCAGGCTGGTGGTGAAATGACTCATGTCATTGCTGGGACACATGGCTACATAGCTCCTGAATATGCATATACGTACAAGGTGAATGAGAAGAGTGATGTGTATAGCTTTGGGGTGGTCTTGATGGAGTTGGTGACAGGAAAGAGGCCAGTGGAGCCTGAGTTTGGGGAGAACAAGGACATAGTGAGCTGGGTTTGCAGTAAAATAACTTGCAAAGATACTGAGCTTGAATCGGTGGACTCGAGCATCGAAGAGGCTCTAAAAGAAGACGCCCTCAACGTTTTGAGCATTGCAATTCGCTGCACCGCTAGGGTTCCGGTGCTAAGACCCTCGATGAGAATGGTGGTTCAAATGCTGGAAGAGGCACAACCTTGTCAGCTCACCAGCATAAGCATCACCAAGGAAGGTGAAAGTAACCCGAAAATGGAGCAGAAATTCCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

980

Amino Acids

107.46

Weight (kDa)

5.77

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 67 9e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 166 - 306 2.9e-13 Leucine-rich repeat region
LRR_14 PF23598 407 - 546 4.3e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 666 - 834 1.3e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 668 - 851 1.5e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 1478
AccB7I CCANNNNNTGG 1 cut(s) 1568
AciI CCGC 3 cut(s) 1780, 2103, 2799
AclI AACGTT 1 cut(s) 2772
AclWI GGATC 1 cut(s) 2304
AcoI YGGCCR 3 cut(s) 1549, 1690, 2184
AcsI RAATTY 7 cut(s) 313, 385, 693, 1410, 1774, 1795, 2927
AcuI CTGAAG 2 cut(s) 1257, 1377
AcvI CACGTG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 2763
AfaI GTAC 9 cut(s) 355, 1016, 1192, 1851, 1952, 2039, 2046, 2273, 2570
AfiI CCNNNNNNNGG 8 cut(s) 337, 413, 861, 1402, 1568, 2328, 2657, 2917
AflII CTTAAG 1 cut(s) 653
AflIII ACRYGT 1 cut(s) 1165
AjnI CCWGG 2 cut(s) 1057, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 1995, 2027
Alw21I GWGCWC 4 cut(s) 93, 213, 849, 948
Alw26I GTCTC 2 cut(s) 220, 876
AlwI GGATC 1 cut(s) 2304
Ama87I CYCGRG 1 cut(s) 2734
AoxI GGCC 6 cut(s) 1549, 1690, 1781, 2184, 2319, 2639
ApoI RAATTY 7 cut(s) 313, 385, 693, 1410, 1774, 1795, 2927
Asp700I GAANNNNTTC 2 cut(s) 389, 1109
AspS9I GGNCC 4 cut(s) 1046, 1156, 1262, 2088
AsuC2I CCSGG 1 cut(s) 911
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 1 cut(s) 2734
AvaII GGWCC 4 cut(s) 1046, 1156, 1262, 2088
AxyI CCTNAGG 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 1701
BalI TGGCCA 2 cut(s) 1551, 2186
BanI GGYRCC 1 cut(s) 1478
BanII GRGCYC 4 cut(s) 93, 213, 849, 948
BbrPI CACGTG 1 cut(s) 174
BbsI GAAGAC 2 cut(s) 1442, 2766
Bbv12I GWGCWC 4 cut(s) 93, 213, 849, 948
BccI CCATC 4 cut(s) 2051, 2320, 2441, 2611
BceAI ACGGC 2 cut(s) 35, 1677
BciT130I CCWGG 2 cut(s) 1059, 1749
BclI TGATCA 1 cut(s) 1912
BcnI CCSGG 1 cut(s) 911
BcoDI GTCTC 2 cut(s) 220, 876
BfaI CTAG 9 cut(s) 453, 1496, 1620, 1809, 1985, 2430, 2444, 2463, 2802
BfmI CTRYAG 3 cut(s) 604, 1100, 2230
BfrI CTTAAG 1 cut(s) 653
BglI GCCNNNNNGGC 1 cut(s) 2183
BmcAI AGTACT 2 cut(s) 1851, 2273
Bme1390I CCNGG 3 cut(s) 911, 1059, 1749
Bme18I GGWCC 4 cut(s) 1046, 1156, 1262, 2088
BmeT110I CYCGRG 1 cut(s) 2734
BmgT120I GGNCC 4 cut(s) 1046, 1156, 1262, 2088
BmiI GGNNCC 6 cut(s) 158, 1264, 1480, 2090, 2649, 2808
BmrFI CCNGG 3 cut(s) 911, 1059, 1749
BmrI ACTGGG 3 cut(s) 195, 764, 1696
BmsI GCATC 5 cut(s) 618, 799, 2164, 2748, 2895
BmuI ACTGGG 3 cut(s) 195, 764, 1696
BoxI GACNNNNGTC 1 cut(s) 2519
BpiI GAAGAC 2 cut(s) 1442, 2766
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 3 cut(s) 417, 683, 1866
Bpu10I CCTNAGC 1 cut(s) 1895
Bpu14I TTCGAA 1 cut(s) 1799
BpuEI CTTGAG 4 cut(s) 509, 755, 833, 848
BpuMI CCSGG 1 cut(s) 911
Bsa29I ATCGAT 1 cut(s) 1324
BsaAI YACGTR 3 cut(s) 174, 1166, 2568
BsaBI GATNNNNATC 1 cut(s) 1911
BsaHI GRCGYC 1 cut(s) 2763
BsaJI CCNNGG 8 cut(s) 476, 520, 1057, 1058, 1817, 2178, 2389, 2892
BsaWI WCCGGW 4 cut(s) 31, 1158, 1486, 2809
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 1574, 1604, 2639, 2669
Bsc4I CCNNNNNNNGG 8 cut(s) 337, 413, 861, 1402, 1568, 2328, 2657, 2917
Bse1I ACTGG 9 cut(s) 190, 400, 590, 770, 976, 1048, 1681, 1702, 2642
Bse21I CCTNAGG 1 cut(s) 797
Bse3DI GCAATG 4 cut(s) 1574, 1713, 2523, 2781
Bse8I GATNNNNATC 1 cut(s) 1911
BseBI CCWGG 2 cut(s) 1059, 1749
BseCI ATCGAT 1 cut(s) 1324
BseDI CCNNGG 8 cut(s) 476, 520, 1057, 1058, 1817, 2178, 2389, 2892
BseGI GGATG 1 cut(s) 1068
BseJI GATNNNNATC 1 cut(s) 1911
BseLI CCNNNNNNNGG 8 cut(s) 337, 413, 861, 1402, 1568, 2328, 2657, 2917
BseMI GCAATG 4 cut(s) 1574, 1713, 2523, 2781
BseNI ACTGG 9 cut(s) 190, 400, 590, 770, 976, 1048, 1681, 1702, 2642
BseRI GAGGAG 3 cut(s) 41, 52, 1915
BseSI GKGCMC 1 cut(s) 1701
BseYI CCCAGC 4 cut(s) 1202, 2092, 2528, 2682
BsgI GTGCAG 5 cut(s) 860, 1777, 2288, 2516, 2779
BshFI GGCC 6 cut(s) 1551, 1692, 1783, 2186, 2321, 2641
BshNI GGYRCC 1 cut(s) 1478
BshVI ATCGAT 1 cut(s) 1324
BsiHKAI GWGCWC 4 cut(s) 93, 213, 849, 948
BsiHKCI CYCGRG 1 cut(s) 2734
BsiSI CCGG 6 cut(s) 13, 32, 911, 1159, 1487, 2810
BslFI GGGAC 4 cut(s) 893, 1181, 1248, 2545
BslI CCNNNNNNNGG 8 cut(s) 337, 413, 861, 1402, 1568, 2328, 2657, 2917
BsmAI GTCTC 2 cut(s) 220, 876
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 4 cut(s) 893, 1181, 1248, 2545
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 6 cut(s) 1551, 1692, 1783, 2186, 2321, 2641
BsoBI CYCGRG 1 cut(s) 2734
Bsp119I TTCGAA 1 cut(s) 1799
Bsp1286I GDGCHC 5 cut(s) 93, 213, 849, 948, 1701
Bsp1407I TGTACA 1 cut(s) 2037
Bsp143I GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
Bsp19I CCATGG 2 cut(s) 476, 2389
BspACI CCGC 3 cut(s) 1780, 2103, 2799
BspANI GGCC 6 cut(s) 1551, 1692, 1783, 2186, 2321, 2641
BspDI ATCGAT 1 cut(s) 1324
BspLI GGNNCC 6 cut(s) 158, 1264, 1480, 2090, 2649, 2808
BspMAI CTGCAG 2 cut(s) 1104, 2234
BspPI GGATC 1 cut(s) 2304
BspT104I TTCGAA 1 cut(s) 1799
BspT107I GGYRCC 1 cut(s) 1478
BspTI CTTAAG 1 cut(s) 653
BsrDI GCAATG 4 cut(s) 1574, 1713, 2523, 2781
BsrGI TGTACA 1 cut(s) 2037
BsrI ACTGG 9 cut(s) 190, 400, 590, 770, 976, 1048, 1681, 1702, 2642
BssECI CCNNGG 8 cut(s) 476, 520, 1057, 1058, 1817, 2178, 2389, 2892
BssMI GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
BssNI GRCGYC 1 cut(s) 2763
BssT1I CCWWGG 5 cut(s) 476, 520, 1817, 2389, 2892
Bst2UI CCWGG 2 cut(s) 1059, 1749
Bst4CI ACNGT 3 cut(s) 605, 1195, 1311
Bst6I CTCTTC 8 cut(s) 59, 261, 909, 984, 1919, 2579, 2739, 2847
BstACI GRCGYC 1 cut(s) 2763
BstAFI CTTAAG 1 cut(s) 653
BstAUI TGTACA 1 cut(s) 2037
BstBAI YACGTR 3 cut(s) 174, 1166, 2568
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 6 cut(s) 669, 1217, 1344, 2255, 2484, 2499
BstDSI CCRYGG 2 cut(s) 476, 2389
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 1068
BstKTI GATC 8 cut(s) 111, 541, 838, 1240, 1669, 1909, 1915, 2299
BstMAI GTCTC 2 cut(s) 220, 876
BstMBI GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
BstNI CCWGG 2 cut(s) 1059, 1749
BstNSI RCATGY 1 cut(s) 2210
BstPAI GACNNNNGTC 1 cut(s) 2519
BstSCI CCNGG 3 cut(s) 909, 1057, 1747
BstSFI CTRYAG 3 cut(s) 604, 1100, 2230
BstSLI GKGCMC 1 cut(s) 1701
BstSNI TACGTA 1 cut(s) 2568
BstV2I GAAGAC 2 cut(s) 1442, 2766
BstXI CCANNNNNNTGG 2 cut(s) 686, 1688
Bsu15I ATCGAT 1 cut(s) 1324
Bsu36I CCTNAGG 1 cut(s) 797
BsuRI GGCC 6 cut(s) 1551, 1692, 1783, 2186, 2321, 2641
BsuTUI ATCGAT 1 cut(s) 1324
BtgI CCRYGG 2 cut(s) 476, 2389
BtsCI GGATG 1 cut(s) 1068
BtsIMutI CAGTG 4 cut(s) 183, 393, 969, 2649
Cac8I GCNNGC 6 cut(s) 669, 1217, 1344, 2255, 2484, 2499
Cfr13I GGNCC 4 cut(s) 1046, 1156, 1262, 2088
ClaI ATCGAT 1 cut(s) 1324
CseI GACGC 2 cut(s) 275, 2771
Csp6I GTAC 9 cut(s) 354, 1015, 1191, 1850, 1951, 2038, 2045, 2272, 2569
CviQI GTAC 9 cut(s) 354, 1015, 1191, 1850, 1951, 2038, 2045, 2272, 2569
DpnI GATC 8 cut(s) 110, 540, 837, 1239, 1668, 1908, 1914, 2298
DpnII GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
EaeI YGGCCR 3 cut(s) 1549, 1690, 2184
Eam1104I CTCTTC 8 cut(s) 59, 261, 909, 984, 1919, 2579, 2739, 2847
EarI CTCTTC 8 cut(s) 59, 261, 909, 984, 1919, 2579, 2739, 2847
Ecl136II GAGCTC 4 cut(s) 91, 211, 847, 946
Eco105I TACGTA 1 cut(s) 2568
Eco130I CCWWGG 5 cut(s) 476, 520, 1817, 2389, 2892
Eco24I GRGCYC 4 cut(s) 93, 213, 849, 948
Eco47I GGWCC 4 cut(s) 1046, 1156, 1262, 2088
Eco53kI GAGCTC 4 cut(s) 91, 211, 847, 946
Eco57I CTGAAG 2 cut(s) 1257, 1377
Eco72I CACGTG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 2734
EcoICRI GAGCTC 4 cut(s) 91, 211, 847, 946
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 1262
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 2 cut(s) 1057, 1747
EcoT14I CCWWGG 5 cut(s) 476, 520, 1817, 2389, 2892
EcoT22I ATGCAT 1 cut(s) 2563
EcoT38I GRGCYC 4 cut(s) 93, 213, 849, 948
ErhI CCWWGG 5 cut(s) 476, 520, 1817, 2389, 2892
Esp3I CGTCTC 1 cut(s) 220
FalI AAGNNNNNCTT 4 cut(s) 105, 137, 2116, 2148
FaqI GGGAC 4 cut(s) 893, 1181, 1248, 2545
FbaI TGATCA 1 cut(s) 1912
FokI GGATG 1 cut(s) 1075
FriOI GRGCYC 4 cut(s) 93, 213, 849, 948
FspBI CTAG 9 cut(s) 453, 1496, 1620, 1809, 1985, 2430, 2444, 2463, 2802
GsaI CCCAGC 4 cut(s) 1206, 2096, 2532, 2686
GsuI CTGGAG 3 cut(s) 417, 683, 1866
HaeIII GGCC 6 cut(s) 1551, 1692, 1783, 2186, 2321, 2641
HapII CCGG 6 cut(s) 13, 32, 911, 1159, 1487, 2810
HgaI GACGC 2 cut(s) 275, 2771
Hin1I GRCGYC 1 cut(s) 2763
HindIII AAGCTT 7 cut(s) 569, 578, 722, 824, 989, 1732, 1963
HpaII CCGG 6 cut(s) 13, 32, 911, 1159, 1487, 2810
Hpy166II GTNNAC 5 cut(s) 354, 1337, 2038, 2266, 2731
Hpy8I GTNNAC 5 cut(s) 354, 1337, 2038, 2266, 2731
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 6 cut(s) 50, 290, 1253, 1670, 2311, 2891
HpyCH4III ACNGT 3 cut(s) 605, 1195, 1311
HpyCH4IV ACGT 4 cut(s) 173, 1165, 2567, 2772
HpySE526I ACGT 4 cut(s) 173, 1165, 2567, 2772
Hsp92I GRCGYC 1 cut(s) 2763
Ksp22I TGATCA 1 cut(s) 1912
Kzo9I GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
LmnI GCTCC 6 cut(s) 664, 1212, 2286, 2554, 2647, 2920
LweI GCATC 5 cut(s) 618, 799, 2164, 2748, 2895
MaeI CTAG 9 cut(s) 453, 1496, 1620, 1809, 1985, 2430, 2444, 2463, 2802
MaeII ACGT 4 cut(s) 173, 1165, 2567, 2772
MalI GATC 8 cut(s) 110, 540, 837, 1239, 1668, 1908, 1914, 2298
MboI GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
MfeI CAATTG 2 cut(s) 680, 1422
MhlI GDGCHC 5 cut(s) 93, 213, 849, 948, 1701
MlsI TGGCCA 2 cut(s) 1551, 2186
MluNI TGGCCA 2 cut(s) 1551, 2186
MlyI GAGTC 8 cut(s) 433, 451, 640, 1484, 1997, 2093, 2509, 2726
Mox20I TGGCCA 2 cut(s) 1551, 2186
Mph1103I ATGCAT 1 cut(s) 2563
MroXI GAANNNNTTC 2 cut(s) 389, 1109
MscI TGGCCA 2 cut(s) 1551, 2186
MseI TTAA 5 cut(s) 654, 1313, 1418, 1725, 1961
MslI CAYNNNNRTG 2 cut(s) 2211, 2418
Msp20I TGGCCA 2 cut(s) 1551, 2186
MspA1I CMGCKG 1 cut(s) 2302
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 6 cut(s) 13, 32, 911, 1159, 1487, 2810
MspR9I CCNGG 3 cut(s) 911, 1059, 1749
MunI CAATTG 2 cut(s) 680, 1422
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 2 cut(s) 1059, 1749
NciI CCSGG 1 cut(s) 911
NcoI CCATGG 2 cut(s) 476, 2389
NdeII GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
NlaIV GGNNCC 6 cut(s) 158, 1264, 1480, 2090, 2649, 2808
NmeAIII GCCGAG 1 cut(s) 2203
NmuCI GTSAC 5 cut(s) 483, 1704, 1811, 2396, 2626
NsiI ATGCAT 1 cut(s) 2563
NspI RCATGY 1 cut(s) 2210
NspV TTCGAA 1 cut(s) 1799
PaeR7I CTCGAG 1 cut(s) 2734
PasI CCCWGGG 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 2 cut(s) 2732, 2769
PctI GAATGC 1 cut(s) 454
PdmI GAANNNNTTC 2 cut(s) 389, 1109
PfeI GAWTC 7 cut(s) 493, 658, 889, 1123, 1130, 1249, 2723
PflMI CCANNNNNTGG 1 cut(s) 1568
PfoI TCCNGGA 1 cut(s) 909
PleI GAGTC 8 cut(s) 432, 450, 640, 1484, 1996, 2093, 2509, 2726
PmaCI CACGTG 1 cut(s) 174
PmlI CACGTG 1 cut(s) 174
PpsI GAGTC 8 cut(s) 432, 450, 640, 1484, 1996, 2093, 2509, 2726
Ppu21I YACGTR 3 cut(s) 174, 1166, 2568
PpuMI RGGWCCY 1 cut(s) 1262
PshAI GACNNNNGTC 1 cut(s) 2519
PsiI TTATAA 1 cut(s) 710
Psp124BI GAGCTC 4 cut(s) 93, 213, 849, 948
Psp1406I AACGTT 1 cut(s) 2772
Psp5II RGGWCCY 1 cut(s) 1262
Psp6I CCWGG 2 cut(s) 1057, 1747
PspCI CACGTG 1 cut(s) 174
PspFI CCCAGC 4 cut(s) 1202, 2092, 2528, 2682
PspGI CCWGG 2 cut(s) 1057, 1747
PspN4I GGNNCC 6 cut(s) 158, 1264, 1480, 2090, 2649, 2808
PspPI GGNCC 4 cut(s) 1046, 1156, 1262, 2088
PspPPI RGGWCCY 1 cut(s) 1262
PspXI VCTCGAGB 1 cut(s) 2734
PstI CTGCAG 2 cut(s) 1104, 2234
PvuII CAGCTG 1 cut(s) 2302
RsaI GTAC 9 cut(s) 355, 1016, 1192, 1851, 1952, 2039, 2046, 2273, 2570
RsaNI GTAC 9 cut(s) 354, 1015, 1191, 1850, 1951, 2038, 2045, 2272, 2569
RseI CAYNNNNRTG 2 cut(s) 2211, 2418
SacI GAGCTC 4 cut(s) 93, 213, 849, 948
SaqAI TTAA 5 cut(s) 654, 1313, 1418, 1725, 1961
Sau3AI GATC 8 cut(s) 108, 538, 835, 1237, 1666, 1906, 1912, 2296
Sau96I GGNCC 4 cut(s) 1046, 1156, 1262, 2088
ScaI AGTACT 2 cut(s) 1851, 2273
SchI GAGTC 8 cut(s) 433, 451, 640, 1484, 1997, 2093, 2509, 2726
ScrFI CCNGG 3 cut(s) 911, 1059, 1749
SduI GDGCHC 5 cut(s) 93, 213, 849, 948, 1701
SfaNI GCATC 5 cut(s) 618, 799, 2164, 2748, 2895
SfcI CTRYAG 3 cut(s) 604, 1100, 2230
Sfr274I CTCGAG 1 cut(s) 2734
SfuI TTCGAA 1 cut(s) 1799
SinI GGWCC 4 cut(s) 1046, 1156, 1262, 2088
SlaI CTCGAG 1 cut(s) 2734
SmiMI CAYNNNNRTG 2 cut(s) 2211, 2418
SmlI CTYRAG 6 cut(s) 488, 653, 734, 827, 848, 2734
SmoI CTYRAG 6 cut(s) 488, 653, 734, 827, 848, 2734
SnaBI TACGTA 1 cut(s) 2568
SsiI CCGC 3 cut(s) 1780, 2103, 2799
SspI AATATT 1 cut(s) 2437
SspMI CTAG 9 cut(s) 453, 1496, 1620, 1809, 1985, 2430, 2444, 2463, 2802
SstI GAGCTC 4 cut(s) 93, 213, 849, 948
StyD4I CCNGG 3 cut(s) 909, 1057, 1747
StyI CCWWGG 5 cut(s) 476, 520, 1817, 2389, 2892
TaaI ACNGT 3 cut(s) 605, 1195, 1311
TaiI ACGT 4 cut(s) 176, 1168, 2570, 2775
TaqI TCGA 9 cut(s) 93, 264, 537, 1324, 1799, 2122, 2735, 2742, 2825
TatI WGTACW 6 cut(s) 353, 1014, 1849, 2037, 2044, 2271
TauI GCSGC 1 cut(s) 1783
TfiI GAWTC 7 cut(s) 493, 658, 889, 1123, 1130, 1249, 2723
Tru1I TTAA 5 cut(s) 654, 1313, 1418, 1725, 1961
Tru9I TTAA 5 cut(s) 654, 1313, 1418, 1725, 1961
TscAI CASTG 4 cut(s) 190, 400, 976, 2649
TseFI GTSAC 5 cut(s) 483, 1704, 1811, 2396, 2626
Tsp45I GTSAC 5 cut(s) 483, 1704, 1811, 2396, 2626
TspDTI ATGAA 9 cut(s) 141, 1262, 1442, 1743, 1784, 1923, 1958, 1992, 2324
TspGWI ACGGA 3 cut(s) 339, 515, 572
TspRI CASTG 4 cut(s) 190, 400, 976, 2649
Van91I CCANNNNNTGG 1 cut(s) 1568
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 4 cut(s) 1046, 1156, 1262, 2088
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 7 cut(s) 313, 385, 693, 1410, 1774, 1795, 2927
XceI RCATGY 1 cut(s) 2210
XhoI CTCGAG 1 cut(s) 2734
XmnI GAANNNNTTC 2 cut(s) 389, 1109
XspI CTAG 9 cut(s) 453, 1496, 1620, 1809, 1985, 2430, 2444, 2463, 2802
ZrmI AGTACT 2 cut(s) 1851, 2273
Zsp2I ATGCAT 1 cut(s) 2563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.