MD05G1112600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011518
Physical Location & Seq
Reverse (-)
23120485 .. 23124018
3534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1112600.v1.1.491

Sequence Viewer

Length: 2922 bp
ATGTCTTTCCGGCAGAGATTACCTCCTCCTCCGACAACCATCCTCCTCCCTCTGCTCTTCCTCTTCCTCTCCCTGTTTTCTTCATCCACATCCGACGAGCTTCAACCTCTCTTAAATCTCAAGCAATCCCTTGAAACCTCAAACCCAAGTCTTTTCACTACTTGGGCACCGCCCAATGGGGTCTGCAACTTCACCGGGATCGTCTGCGACTCCGACGGCTTTGTCTCCGAAATCAACCTCTCCCAGCAAAACCTTTCCGGGTTTCTTCCGTTGCACGCAATCTGCTCGCTTCCATCGTTAAAAAAGCTTTCTTTGGGCTCCAATGGATTGCACGGAAGCCTCACGGATGACCTGAAAAACTGCTCGAGCTTGGAGCAGTTGGATTTGGGTAAAAATTCGTTTACCGGAAGAGTTCCAGACTTGTCTTCTTTGACAAAATTAACCCTCCTGAGTCTCAATGGCAGCGGATTTTCCGGGAAATTTCCATGGAAATCGCTGGAAAATCTCACCAGCTTAGCTTTCTTGAGCCTCGGCGACAACCCTTTTGATCAAACTCCATTTCCTGAAGAAGTTGTGAAGCTTGAAAAGCTTTACTGGCTGTACCTCACAAATTGCAGCATCGCCGGAAAAATCCCAGAGGAGATCGGAAACCTCACTCTGCTCGAAAACCTCGAGCTTTCGGACAACCAACTGTTGGGACAAATTCCCCAGAGAATTTCAAATCTCGGGAAGCTATGGCAGCTCGAGCTTTACAACAATTCCCTGACCGGAAAAATCCCGGTTGGATTCGGCAACCTCACAAGCCTTGTCAATTTTGATGCCTCAGCTAACAAGCTTGAAGGTGGCCTCTCTGAGCTGAGGTTTTTGACAAGGCTTGAGTCTCTGCAATTGTTCGAAAACCGGTTAGAAGGCGAGGTTCCGGCGGAGTTTGGGGAGTTGAAGAGCCTTTCCAAGCTCTCACTTTACACGAACAAGCTCACCGGTACACTTCCTCAAAAGCTCGGTTCTTGGGCGGGATTGGATTACATTGACGTGTCGGAGAACTATCTGACTGGTGCTATACCGCCCGGAATGTGCAACAATGGGAAGATGATTGATTTTCTCCTTCTGCAGAACAATTTCACAGGTGGGATTCCTGAGAGCTATGCCAATTGTAAGTCTTTGGTTCGGATTCGTGTGAGCAAAAATTCGCTTTGGGGCACTGTTCCTGCTGGATTATGGAGCTTGCCAAATGTTGTTCTCATTGATCTTTCTATGAATCAATTTGAAGGTCCTCTGGCACCAGAGATTGGTAAGGCAAACTCTTTGTCACTATTACTTTTAGCGAATAATCGATTTTCGGGTGAATTGCCTGATGCCCTGTCTGAAGCTACCTCATTGATCGCAATTCAAATGAGTGTGAACCAATTTAGTGGGAAAATCCCAGAAGCAATTGGGAAATTGACAAAATTAAGCAGTCTTGATTTGGACCAAAATATGTTGTCTGGTACAATACCAGAATCATTAGGCTCATGTTTTGGTATTTCTGAAATAAACCTTGCCCACAATTATATTTCTGGTGAAATCCCATCAAGTCTTGGATCATTGCATAATCTGAATTCCTTGAACTTATCGGCAAACCGCCTTTCCAGCGAAATTCCTGCTACTTTTTCGTCACTAAAACTCAGCCTTCTTGATCTGACAAACAACCAGTTGACTGGTCGAATCCCCGAATCACTGTCCATTCAGGCCTTTAATGAAAGCTTTGATGGAAATCCGGGTTTGTGCAGTCAGAACATGGAAAATGTGCGGTCTTGTTCCTCAAGTCCTGGCACTTCTAGCCGGCCCCGAATTTTCCTGTCCAGTTTCATTGCATCAATACTTGTGCTGCTTGTTGTGCTTGGTTTGCTCTTGCTGTTGAAGCTGAGGAAGAGCAAACTTGACCGTCCGCTAAAGAGTGATTCGTGGACTATGAAGCAATACCATGTGCTAAGCTTCACAGAGAAGGAAATCTTAGATTCAGTGAAAGCTGAGAACTTGATTGGCAAGGGGGGGTCAGGGAATGTATACAAAGCTGTGCTAAGTGATGGGAGAGAATTTGCTGTAAAGCACATTTGGACCACTTCAGACTCCGGTGACAGAAAAAGTTACCGAAGCAGCGCTTCCATCTTGAAGAAGACTAAATCCGGGTCATCAGAGTACGGTGCAGAAGTGGCCACACTGAGCTCTCTGAGGCATGTGAATGTGGTGAAGCTCTACTGCAGCATATCCAGTGAGGACAGCAACCTGCTGGTTTACGAGTACTTTCCAAACGGGAGCTTATATGATCAGCTACACACAAGTAAAAAGATGGAGATGGGGTGGGAAGTAAGGTATGAGATTGCGTTGGGGGCTGCAAGGGGTTTGGAGTATCTTCACCATGGAAGCAGTCATAGACCTGTGATACACAGGGATGTGAAGTCTAGTAATATTCTGCTGGATGGAGATTGGAAGCCAAGAATTGCTGATTTTGGACTTGCAAAGATTATGCAGGCTGGTGTAGATTGCACGCATGTCATTGCCGGGACACTTGGGTACATAGCTCCAGAGTATGCATATACCTGCAAGATCAATGAGAAGAGTGACGTTTATAGCTTCGGGGTGGTGTTGATGGAGTTGGTCACCGGGAAGAGGCCAGTTGAGGCTGAGTTCGGAGAGAACACGGATATAGTGAGCTGGGTCTGCAGTAAAATGCGGTGCGTTGATAGTGAGCTTGAATTGGTGGACAGGAGCATTTCTGACGATCTAAAACAAGATGCCATCAATGTTTTGACCATAGCAGTCCGCTGCACTGCCAAGGTGCCGGTTATAAGACCCTCCATGAGAATGGTGGTTCAAATGCTGGAAGAGGCGGAGCCTTATAAACTCAACTGCATAAGCATTGCCAAAGAAGCTGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

106.49

Weight (kDa)

5.67

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 190 - 310 2.2e-12 Leucine-rich repeat region
LRR_8 PF13855 509 - 565 3.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 669 - 953 8.1e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 671 - 952 2.7e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2828, 2880
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 2274, 2588
AccB1I GGYRCC 3 cut(s) 166, 1279, 2818
AccB7I CCANNNNNTGG 2 cut(s) 694, 1289
AccI GTMKAC 1 cut(s) 2046
AclWI GGATC 2 cut(s) 206, 1588
AcoI YGGCCR 1 cut(s) 2193
AcsI RAATTY 9 cut(s) 394, 479, 702, 714, 1186, 1597, 1635, 1830, 2075
AcuI CTGAAG 3 cut(s) 585, 1386, 2088
AfaI GTAC 6 cut(s) 602, 985, 1489, 2180, 2282, 2555
AfeI AGCGCT 1 cut(s) 2140
AfiI CCNNNNNNNGG 4 cut(s) 176, 694, 1289, 2649
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 2 cut(s) 900, 980
AjiI CACGTC 1 cut(s) 1033
AjnI CCWGG 1 cut(s) 1807
Alw21I GWGCWC 1 cut(s) 2207
Alw26I GTCTC 3 cut(s) 229, 458, 885
AlwI GGATC 2 cut(s) 206, 1588
Ama87I CYCGRG 4 cut(s) 364, 671, 725, 743
Aor51HI AGCGCT 1 cut(s) 2140
AoxI GGCC 5 cut(s) 844, 1728, 1823, 2193, 2651
ApeKI GCWGC 8 cut(s) 462, 615, 739, 1867, 2136, 2241, 2372, 2805
ApoI RAATTY 9 cut(s) 394, 479, 702, 714, 1186, 1597, 1635, 1830, 2075
ArsI GACNNNNNNTTYG 4 cut(s) 527, 559, 1292, 1324
AsiGI ACCGGT 2 cut(s) 900, 980
Asp700I GAANNNNTTC 1 cut(s) 1118
AspLEI GCGC 1 cut(s) 2141
AspS9I GGNCC 4 cut(s) 1271, 1468, 1824, 2097
AsuC2I CCSGG 9 cut(s) 196, 259, 475, 779, 1068, 1758, 2167, 2542, 2644
AsuHPI GGTGA 9 cut(s) 184, 499, 970, 1355, 1571, 2126, 2239, 2387, 2632
AsuII TTCGAA 1 cut(s) 894
AvaI CYCGRG 4 cut(s) 364, 671, 725, 743
AvaII GGWCC 3 cut(s) 1271, 1468, 2097
BaeGI GKGCMC 2 cut(s) 169, 1202
BalI TGGCCA 1 cut(s) 2195
BanI GGYRCC 3 cut(s) 166, 1279, 2818
BanII GRGCYC 2 cut(s) 320, 2207
BbsI GAAGAC 2 cut(s) 417, 2162
Bbv12I GWGCWC 1 cut(s) 2207
BbvCI CCTCAGC 3 cut(s) 823, 857, 1904
BbvI GCAGC 8 cut(s) 474, 627, 751, 1854, 2148, 2253, 2359, 2792
BceAI ACGGC 1 cut(s) 232
BcgI CGANNNNNNTGC 4 cut(s) 267, 301, 1622, 1656
BciT130I CCWGG 1 cut(s) 1809
BclI TGATCA 2 cut(s) 547, 2305
BcnI CCSGG 9 cut(s) 196, 259, 475, 779, 1068, 1758, 2167, 2542, 2644
BcoDI GTCTC 3 cut(s) 229, 458, 885
BfaI CTAG 2 cut(s) 1818, 2442
BfmI CTRYAG 3 cut(s) 1109, 2239, 2701
BfoI RGCGCY 1 cut(s) 2142
BfuAI ACCTGC 2 cut(s) 2274, 2588
BisI GCNGC 8 cut(s) 463, 616, 740, 1868, 2137, 2242, 2373, 2806
BlpI GCTNAGC 2 cut(s) 514, 1970
BlsI GCNGC 8 cut(s) 464, 617, 741, 1869, 2138, 2243, 2374, 2807
BmcAI AGTACT 1 cut(s) 2282
Bme18I GGWCC 3 cut(s) 1271, 1468, 2097
BmeT110I CYCGRG 4 cut(s) 364, 671, 725, 743
BmgBI CACGTC 1 cut(s) 1033
BmgT120I GGNCC 4 cut(s) 1271, 1468, 1824, 2097
BmiI GGNNCC 7 cut(s) 168, 319, 918, 1281, 1826, 2820, 2874
BmsI GCATC 5 cut(s) 627, 808, 1345, 1862, 2764
BpiI GAAGAC 2 cut(s) 417, 2162
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 2547
Bpu10I CCTNAGC 3 cut(s) 823, 857, 1904
Bpu1102I GCTNAGC 2 cut(s) 514, 1970
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 4 cut(s) 104, 544, 896, 1786
BpuMI CCSGG 9 cut(s) 196, 259, 475, 779, 1068, 1758, 2167, 2542, 2644
Bsa29I ATCGAT 1 cut(s) 1333
BsaBI GATNNNNATC 1 cut(s) 1752
BsaJI CCNNGG 4 cut(s) 485, 529, 2398, 2814
BsaWI WCCGGW 5 cut(s) 404, 767, 900, 980, 2111
BsaXI ACNNNNNCTCC 2 cut(s) 2324, 2354
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 694, 1289, 2649
Bse118I RCCGGY 4 cut(s) 900, 980, 1821, 2821
Bse1I ACTGG 7 cut(s) 599, 1057, 1690, 1702, 1842, 2250, 2654
Bse3DI GCAATG 4 cut(s) 1583, 1848, 2535, 2898
Bse8I GATNNNNATC 1 cut(s) 1752
BseBI CCWGG 1 cut(s) 1809
BseCI ATCGAT 1 cut(s) 1333
BseDI CCNNGG 4 cut(s) 485, 529, 2398, 2814
BseGI GGATG 6 cut(s) 39, 83, 89, 352, 2437, 2464
BseJI GATNNNNATC 1 cut(s) 1752
BseLI CCNNNNNNNGG 4 cut(s) 176, 694, 1289, 2649
BseMI GCAATG 4 cut(s) 1583, 1848, 2535, 2898
BseNI ACTGG 7 cut(s) 599, 1057, 1690, 1702, 1842, 2250, 2654
BseRI GAGGAG 4 cut(s) 15, 18, 35, 653
BseSI GKGCMC 2 cut(s) 169, 1202
BseXI GCAGC 8 cut(s) 474, 627, 751, 1854, 2148, 2253, 2359, 2792
BseYI CCCAGC 2 cut(s) 243, 2694
BsgI GTGCAG 3 cut(s) 1786, 2205, 2791
BshFI GGCC 5 cut(s) 846, 1730, 1825, 2195, 2653
BshNI GGYRCC 3 cut(s) 166, 1279, 2818
BshTI ACCGGT 2 cut(s) 900, 980
BshVI ATCGAT 1 cut(s) 1333
BsiHKAI GWGCWC 1 cut(s) 2207
BsiHKCI CYCGRG 4 cut(s) 364, 671, 725, 743
BslFI GGGAC 2 cut(s) 711, 2557
BslI CCNNNNNNNGG 4 cut(s) 176, 694, 1289, 2649
BsmAI GTCTC 3 cut(s) 229, 458, 885
BsmFI GGGAC 2 cut(s) 711, 2557
BsnI GGCC 5 cut(s) 846, 1730, 1825, 2195, 2653
BsoBI CYCGRG 4 cut(s) 364, 671, 725, 743
Bsp119I TTCGAA 1 cut(s) 894
Bsp1286I GDGCHC 4 cut(s) 169, 320, 1202, 2207
Bsp1720I GCTNAGC 2 cut(s) 514, 1970
Bsp19I CCATGG 2 cut(s) 485, 2398
BspANI GGCC 5 cut(s) 846, 1730, 1825, 2195, 2653
BspDI ATCGAT 1 cut(s) 1333
BspLI GGNNCC 7 cut(s) 168, 319, 918, 1281, 1826, 2820, 2874
BspMAI CTGCAG 3 cut(s) 1113, 2243, 2705
BspMI ACCTGC 2 cut(s) 2274, 2588
BspPI GGATC 2 cut(s) 206, 1588
BspQI GCTCTTC 3 cut(s) 62, 935, 1904
BspT104I TTCGAA 1 cut(s) 894
BspT107I GGYRCC 3 cut(s) 166, 1279, 2818
BsrDI GCAATG 4 cut(s) 1583, 1848, 2535, 2898
BsrFI RCCGGY 4 cut(s) 900, 980, 1821, 2821
BsrI ACTGG 7 cut(s) 599, 1057, 1690, 1702, 1842, 2250, 2654
BssAI RCCGGY 4 cut(s) 900, 980, 1821, 2821
BssECI CCNNGG 4 cut(s) 485, 529, 2398, 2814
BssNAI GTATAC 1 cut(s) 2047
BssT1I CCWWGG 3 cut(s) 485, 2398, 2814
Bst1107I GTATAC 1 cut(s) 2047
Bst2UI CCWGG 1 cut(s) 1809
Bst4CI ACNGT 5 cut(s) 693, 1204, 1719, 1925, 2183
Bst6I CTCTTC 8 cut(s) 62, 68, 403, 935, 1904, 2591, 2642, 2859
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 6 cut(s) 276, 287, 1226, 1823, 2511, 2528
BstDSI CCRYGG 2 cut(s) 485, 2398
BstEII GGTNACC 1 cut(s) 2638
BstF5I GGATG 6 cut(s) 39, 83, 89, 352, 2437, 2464
BstH2I RGCGCY 1 cut(s) 2142
BstHHI GCGC 1 cut(s) 2141
BstMAI GTCTC 3 cut(s) 229, 458, 885
BstNI CCWGG 1 cut(s) 1809
BstNSI RCATGY 2 cut(s) 2219, 2534
BstPI GGTNACC 1 cut(s) 2638
BstSFI CTRYAG 3 cut(s) 1109, 2239, 2701
BstSLI GKGCMC 2 cut(s) 169, 1202
BstV1I GCAGC 8 cut(s) 474, 627, 751, 1854, 2148, 2253, 2359, 2792
BstV2I GAAGAC 2 cut(s) 417, 2162
BstXI CCANNNNNNTGG 3 cut(s) 1412, 1697, 2845
BstZ17I GTATAC 1 cut(s) 2047
Bsu15I ATCGAT 1 cut(s) 1333
BsuRI GGCC 5 cut(s) 846, 1730, 1825, 2195, 2653
BsuTUI ATCGAT 1 cut(s) 1333
BtgI CCRYGG 2 cut(s) 485, 2398
BtgZI GCGATG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 1033
BtsCI GGATG 6 cut(s) 39, 83, 89, 352, 2437, 2464
BtsI GCAGTG 1 cut(s) 2808
BtsIMutI CAGTG 6 cut(s) 1200, 1715, 2007, 2198, 2257, 2808
BveI ACCTGC 2 cut(s) 2274, 2588
Cac8I GCNNGC 6 cut(s) 276, 287, 1226, 1823, 2511, 2528
CfoI GCGC 1 cut(s) 2141
Cfr10I RCCGGY 4 cut(s) 900, 980, 1821, 2821
Cfr13I GGNCC 4 cut(s) 1271, 1468, 1824, 2097
ClaI ATCGAT 1 cut(s) 1333
Csp6I GTAC 6 cut(s) 601, 984, 1488, 2179, 2281, 2554
CspAI ACCGGT 2 cut(s) 900, 980
CviAII CATG 8 cut(s) 486, 1512, 1777, 1964, 2216, 2399, 2531, 2839
CviQI GTAC 6 cut(s) 601, 984, 1488, 2179, 2281, 2554
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 2193
Eam1104I CTCTTC 8 cut(s) 62, 68, 403, 935, 1904, 2591, 2642, 2859
EarI CTCTTC 8 cut(s) 62, 68, 403, 935, 1904, 2591, 2642, 2859
EciI GGCGGA 2 cut(s) 938, 2885
Ecl136II GAGCTC 1 cut(s) 2205
Eco130I CCWWGG 3 cut(s) 485, 2398, 2814
Eco147I AGGCCT 1 cut(s) 1730
Eco24I GRGCYC 2 cut(s) 320, 2207
Eco47I GGWCC 3 cut(s) 1271, 1468, 2097
Eco47III AGCGCT 1 cut(s) 2140
Eco53kI GAGCTC 1 cut(s) 2205
Eco57I CTGAAG 3 cut(s) 585, 1386, 2088
Eco88I CYCGRG 4 cut(s) 364, 671, 725, 743
Eco91I GGTNACC 1 cut(s) 2638
EcoICRI GAGCTC 1 cut(s) 2205
EcoO109I RGGNCCY 1 cut(s) 1271
EcoO65I GGTNACC 1 cut(s) 2638
EcoRI GAATTC 1 cut(s) 1597
EcoRII CCWGG 1 cut(s) 1807
EcoT14I CCWWGG 3 cut(s) 485, 2398, 2814
EcoT22I ATGCAT 1 cut(s) 2575
EcoT38I GRGCYC 2 cut(s) 320, 2207
ErhI CCWWGG 3 cut(s) 485, 2398, 2814
FaeI CATG 8 cut(s) 489, 1515, 1780, 1967, 2219, 2402, 2534, 2842
FalI AAGNNNNNCTT 4 cut(s) 1976, 2008, 2125, 2157
FaqI GGGAC 2 cut(s) 711, 2557
FatI CATG 8 cut(s) 485, 1511, 1776, 1963, 2215, 2398, 2530, 2838
FauI CCCGC 1 cut(s) 1006
FbaI TGATCA 2 cut(s) 547, 2305
FblI GTMKAC 1 cut(s) 2046
Fnu4HI GCNGC 8 cut(s) 463, 616, 740, 1868, 2137, 2242, 2373, 2806
FokI GGATG 6 cut(s) 26, 70, 76, 359, 2444, 2471
FriOI GRGCYC 2 cut(s) 320, 2207
Fsp4HI GCNGC 8 cut(s) 463, 616, 740, 1868, 2137, 2242, 2373, 2806
FspBI CTAG 2 cut(s) 1818, 2442
GlaI GCGC 1 cut(s) 2140
GluI GCNGC 8 cut(s) 463, 616, 740, 1868, 2137, 2242, 2373, 2806
GsaI CCCAGC 2 cut(s) 247, 2698
GsuI CTGGAG 1 cut(s) 2547
HaeII RGCGCY 1 cut(s) 2142
HaeIII GGCC 5 cut(s) 846, 1730, 1825, 2195, 2653
HhaI GCGC 1 cut(s) 2141
Hin1II CATG 8 cut(s) 489, 1515, 1780, 1967, 2219, 2402, 2534, 2842
Hin6I GCGC 1 cut(s) 2139
HinP1I GCGC 1 cut(s) 2139
HincII GTYRAC 1 cut(s) 1695
HindII GTYRAC 1 cut(s) 1695
HindIII AAGCTT 6 cut(s) 305, 578, 587, 833, 1741, 1972
HphI GGTGA 9 cut(s) 184, 499, 970, 1355, 1571, 2126, 2239, 2387, 2632
Hpy166II GTNNAC 8 cut(s) 402, 986, 1402, 1695, 1947, 2047, 2275, 2743
Hpy8I GTNNAC 8 cut(s) 402, 986, 1402, 1695, 1947, 2047, 2275, 2743
Hpy99I CGWCG 2 cut(s) 98, 218
HpyAV CCTTC 6 cut(s) 833, 902, 1115, 1262, 1679, 1978
HpyCH4III ACNGT 5 cut(s) 693, 1204, 1719, 1925, 2183
HpyCH4IV ACGT 2 cut(s) 1032, 2604
HpySE526I ACGT 2 cut(s) 1032, 2604
Hsp92II CATG 8 cut(s) 489, 1515, 1780, 1967, 2219, 2402, 2534, 2842
HspAI GCGC 1 cut(s) 2139
KroI GCCGGC 1 cut(s) 1821
KroNI GCCGGC 1 cut(s) 1823
Ksp22I TGATCA 2 cut(s) 547, 2305
LguI GCTCTTC 3 cut(s) 62, 935, 1904
LmnI GCTCC 7 cut(s) 323, 373, 1221, 2295, 2566, 2748, 2872
Lsp1109I GCAGC 8 cut(s) 474, 627, 751, 1854, 2148, 2253, 2359, 2792
LweI GCATC 5 cut(s) 627, 808, 1345, 1862, 2764
MaeI CTAG 2 cut(s) 1818, 2442
MaeII ACGT 2 cut(s) 1032, 2604
MaeIII GTNAC 6 cut(s) 1308, 1653, 2114, 2126, 2600, 2638
MfeI CAATTG 3 cut(s) 887, 1150, 1431
MhlI GDGCHC 4 cut(s) 169, 320, 1202, 2207
MlsI TGGCCA 1 cut(s) 2195
MluNI TGGCCA 1 cut(s) 2195
MlyI GAGTC 4 cut(s) 203, 460, 887, 2102
MmeI TCCRAC 6 cut(s) 56, 117, 237, 360, 763, 1017
Mox20I TGGCCA 1 cut(s) 2195
Mph1103I ATGCAT 1 cut(s) 2575
MroNI GCCGGC 1 cut(s) 1821
MroXI GAANNNNTTC 1 cut(s) 1118
MscI TGGCCA 1 cut(s) 2195
MseI TTAA 5 cut(s) 113, 299, 440, 1451, 1734
MslI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
Msp20I TGGCCA 1 cut(s) 2195
MspA1I CMGCKG 2 cut(s) 465, 2805
MunI CAATTG 3 cut(s) 887, 1150, 1431
MvaI CCWGG 1 cut(s) 1809
NaeI GCCGGC 1 cut(s) 1823
NciI CCSGG 9 cut(s) 196, 259, 475, 779, 1068, 1758, 2167, 2542, 2644
NcoI CCATGG 2 cut(s) 485, 2398
NgoMIV GCCGGC 1 cut(s) 1821
NlaIII CATG 8 cut(s) 489, 1515, 1780, 1967, 2219, 2402, 2534, 2842
NlaIV GGNNCC 7 cut(s) 168, 319, 918, 1281, 1826, 2820, 2874
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 5 cut(s) 1308, 1653, 2114, 2600, 2638
NsiI ATGCAT 1 cut(s) 2575
NspI RCATGY 2 cut(s) 2219, 2534
NspV TTCGAA 1 cut(s) 894
PaeR7I CTCGAG 3 cut(s) 364, 671, 743
PceI AGGCCT 1 cut(s) 1730
PciSI GCTCTTC 3 cut(s) 62, 935, 1904
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 669
PdiI GCCGGC 1 cut(s) 1823
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 9 cut(s) 786, 1132, 1171, 1258, 1499, 1704, 1712, 1940, 1997
PflMI CCANNNNNTGG 2 cut(s) 694, 1289
PfoI TCCNGGA 1 cut(s) 473
PinAI ACCGGT 2 cut(s) 900, 980
PkrI GCNGC 8 cut(s) 464, 617, 741, 1869, 2138, 2243, 2374, 2807
PleI GAGTC 4 cut(s) 203, 459, 886, 2102
PpsI GAGTC 4 cut(s) 203, 459, 886, 2102
PpuMI RGGWCCY 1 cut(s) 1271
PsiI TTATAA 2 cut(s) 2828, 2880
Psp124BI GAGCTC 1 cut(s) 2207
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 1 cut(s) 1807
PspEI GGTNACC 1 cut(s) 2638
PspFI CCCAGC 2 cut(s) 243, 2694
PspGI CCWGG 1 cut(s) 1807
PspN4I GGNNCC 7 cut(s) 168, 319, 918, 1281, 1826, 2820, 2874
PspPI GGNCC 4 cut(s) 1271, 1468, 1824, 2097
PspPPI RGGWCCY 1 cut(s) 1271
PspXI VCTCGAGB 3 cut(s) 364, 671, 743
PstI CTGCAG 3 cut(s) 1113, 2243, 2705
RsaI GTAC 6 cut(s) 602, 985, 1489, 2180, 2282, 2555
RsaNI GTAC 6 cut(s) 601, 984, 1488, 2179, 2281, 2554
RseI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SacI GAGCTC 1 cut(s) 2207
SapI GCTCTTC 3 cut(s) 62, 935, 1904
SaqAI TTAA 5 cut(s) 113, 299, 440, 1451, 1734
SatI GCNGC 8 cut(s) 463, 616, 740, 1868, 2137, 2242, 2373, 2806
Sau96I GGNCC 4 cut(s) 1271, 1468, 1824, 2097
ScaI AGTACT 1 cut(s) 2282
SchI GAGTC 4 cut(s) 203, 460, 887, 2102
SduI GDGCHC 4 cut(s) 169, 320, 1202, 2207
SfaNI GCATC 5 cut(s) 627, 808, 1345, 1862, 2764
SfcI CTRYAG 3 cut(s) 1109, 2239, 2701
Sfr274I CTCGAG 3 cut(s) 364, 671, 743
SfuI TTCGAA 1 cut(s) 894
SinI GGWCC 3 cut(s) 1271, 1468, 2097
SlaI CTCGAG 3 cut(s) 364, 671, 743
SmiMI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SmlI CTYRAG 7 cut(s) 119, 364, 523, 671, 743, 875, 1801
SmoI CTYRAG 7 cut(s) 119, 364, 523, 671, 743, 875, 1801
SseBI AGGCCT 1 cut(s) 1730
SspI AATATT 1 cut(s) 2449
SspMI CTAG 2 cut(s) 1818, 2442
SstI GAGCTC 1 cut(s) 2207
StuI AGGCCT 1 cut(s) 1730
StyI CCWWGG 3 cut(s) 485, 2398, 2814
TaaI ACNGT 5 cut(s) 693, 1204, 1719, 1925, 2183
TaiI ACGT 2 cut(s) 1035, 2607
TaqI TCGA 7 cut(s) 365, 663, 672, 744, 894, 1333, 1702
TatI WGTACW 1 cut(s) 2280
TfiI GAWTC 9 cut(s) 786, 1132, 1171, 1258, 1499, 1704, 1712, 1940, 1997
Tru1I TTAA 5 cut(s) 113, 299, 440, 1451, 1734
Tru9I TTAA 5 cut(s) 113, 299, 440, 1451, 1734
TscAI CASTG 6 cut(s) 1207, 1722, 2007, 2205, 2257, 2815
TseFI GTSAC 5 cut(s) 1308, 1653, 2114, 2600, 2638
TseI GCWGC 8 cut(s) 462, 615, 739, 1867, 2136, 2241, 2372, 2805
Tsp45I GTSAC 5 cut(s) 1308, 1653, 2114, 2600, 2638
TspDTI ATGAA 5 cut(s) 72, 1271, 1752, 1837, 1967
TspGWI ACGGA 4 cut(s) 258, 348, 359, 2696
TspRI CASTG 6 cut(s) 1207, 1722, 2007, 2205, 2257, 2815
Van91I CCANNNNNTGG 2 cut(s) 694, 1289
VpaK11BI GGWCC 3 cut(s) 1271, 1468, 2097
XapI RAATTY 9 cut(s) 394, 479, 702, 714, 1186, 1597, 1635, 1830, 2075
XceI RCATGY 2 cut(s) 2219, 2534
XcmI CCANNNNNNNNNTGG 1 cut(s) 2845
XhoI CTCGAG 3 cut(s) 364, 671, 743
XmiI GTMKAC 1 cut(s) 2046
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 2 cut(s) 1818, 2442
ZrmI AGTACT 1 cut(s) 2282
Zsp2I ATGCAT 1 cut(s) 2575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.