pycom10g09960

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011518
Physical Location & Seq
Reverse (-)
13213967 .. 13217090
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09960.1

Sequence Viewer

Length: 2922 bp
ATGTCGTTCAGGCAGAGACTCCGTCCGACGACGACAACCATCCTTCTCCCTCTGCTCTTCCTCTTCATCGCCCTGTTTTCTCCATCCACATCCGACGAGCTTCAACCGCTCTTAAACCTCAAGCAATACCTTGAAAACTCAAACCCAAGTCTTTTCACTTCTTGGGCACCACCAAATGGCGTCTGCAACTTCAGCGGCATCGTCTGCGGCTCCGACGGCCTCGTCTCCCAAATCAACCTCTCGCAGCAAAACCTTTCTGGGTTTCTTCCACTGCACGCAATCTGCTCGCTTCCATCGTTAAAAAAGCTTTCTTTGGGATCCAATGGCTTGTACGGAAGCCTCACGGACGACCTGAAAAACTGCACGAGCTTGGAGCAGTTGGATTTGGGTAAAAATTCGTTTACTGGAAAAGTTCCTGACTTGTCTGCTTTAACAAAATTAACCCTCCTGAGTCTCAATGGCAGCAGATTTTCCGGGAAATTTCCGTGGAAATCCCTGGAGAACCTGACCAGGTTAACTTTCTTGAGCCTCGGCGACAACCCTTTTGATCAAACTTCATTTCCTGAAGAAACTGTGAGGCTTGATAAGCTTTACTGGCTGTACCTCTCCAATTGCAGCATCGCCGGAAAAATCCCAGATGGAATCGGAAACCTCACTCTGCTCGAAAACCTCGAGCTTTCGGACAACCAACTGTCCGGCGAGATTCCCCAGAGAATTTCAAATCTCAGGAAGCTATGGCAGCTCGAGCTTTACAGAAACTTTCTGACCGGAAAAATACCGGTTGGATTCGCCAACCTCACAAGCCTTGTCAATTTTGATGCCTCAACTAACAAGCTTGAAGGTGGCCTCTCTGAGCTGAGGTTCTTGACAAAGCTCGAGTCTCTGCATTTGTTCGAAAACCGATTAGAAGGCGAGGTTCCGGCGGAGTTTGGGGAGTTGAAGAACCTTTCCGAGCTCTCACTTTACAGGAACAAGCTCACCGGTGCTCTTCCTCAAAAGCTCGGTTCTTGGGCGGGATTGGCTTACATTGACGTGTCGGAGAACTATCTGACTGGTGCTATACCGCCCGGAATGTGTAACAATGGGAAAATGATCGATTTTCTTCTGCTGCAGAACAATTTCACAGGTGGAATTCCTGACAGCTATGCCAATTGTAAGTCTTTGGTTCGGATTCGTGTGAGTAAAAATTCGCTTTCGGGTTCAATCCCTGCTGGATTATGGAGCTTGCCAAATGTTGTTGTCATTGATCTTTCTATGAATCAGTTTGAAGGTCCTCTGGCACCAGAAATTGGTAAGGCAAACTCTCTGTCACTATTGCTTTTAGCGAATAATCGATTTTCGGGTGAATTGCCAGATGCTTTGTCTGAAGCGACCTCATTGATCACTATTCAGATGAGTGTGAACCAATTTAGTGGGAAAATCCCAGAAACAATTGGGAGTTTGAAGAAATTAAGCAGTCTTGATTTGGACCAAAATATGTTGTCTGGAACTATGCCGGAATCATTAGGCTCATGTTTCGGTATTTCTGAAATAAACCTTGCCCACAACTATATTTCTGGCGAAATCCCATCAAGTCTTGGATCATTGCATAATCTGAATTCCTTGAACTTATCTGGAAACCGGCTTTCCAACGAAATTCCTGCTACTTTGTCTTCCCTAAAGCTCAGCCTTCTTGATCTGACAAATAACCAGTTGATTGGTCGAATCCCCGAAGCATTGTCAATTCAGGCCTTCAATGAAAGCTTTGATGGAAATCCGGGTTTGTGCAGTCAGAACATGGAAAATGTGAGGTCTTGTCCTTTCAAGTCTGGCACTTCTAGCCGACCCCGGATATTCTTGTCCAGTTTCATTGCTTCAGTACTTGTCGTGCTTGTTTTGCTCTGTTTGTTCTTGCTCTTGAAGCTGAGGAGGAGCAATCTTGACCATCCATTGAAGAGCAATTCATGGACTATGAAGCCGTACCATGTGCTAAGCTTTACAGAGAAGGAAATCTTAGACTCGGTGAAAGCTGAGAACTTAATTGGCAAAGGAGGGTCAGGGAATGTATACAAAGTTGTATTGAGTGATGGGAAAGAACTCGCTGTAAAGCACATTTGGACCACTTCAGACTCAGGTGACAGAAAAAGCTGCCGAAGCAGTGCTTCCATCTTGAAGAAGTCTAAATCGGGGTCATCAGAGTATGACTCAGAAGTGGCTACACTGAGCTCTCTGAGGCATGTGAATGTGGTGAAGCTTTACTGCAGCATATCAAGTGAGGACAGTAATCTTCTGGTTTACGAGTACTTTCCTAACGGGAGCTTATATGATCAGCTGCACACAAGTAAGAAGATGAAGATGGGGTGGGAGGTGAGGTATGAGATTGCGGTGGGGGCTGCGAGGGGGTTGGAGTATCTTCACCATGGTAGCAGTCATAGACCTGTGATACACAGGGATGTGAAGTCTAGTAATATTCTGCTGGATGGAGATTGGAAGCCAAGAATTGCTGATTTTGGTCTCGCGAAGATTATGCAGGCTGGTGTAGATTGCACTCAGGTCATTGCTGGGACACTTGGGTACATAGCTCCAGAGTATGCATATACCTGCAAGATCAATGAGAAGAGCGACGTTTATAGCTTCGGGGTGGTGTTGATGGAGTTGGTCACCGGGAAGAAGCCTGTTGAGGCTGTGTTCGGAGAGAACAATGATATAGTGAGCTGGGTATACAGCAAAATGCAGTGCGATGATAGTGTGCTTGAATTGGTGGACACGAGCATTTCTGACGATCTCAAAGAAGATGCCATCAATGTTTTGAGCATCGCAGTCCGCTGCACTGCCAAGATGCCGGTTCTAAGACCCTCCATGAGAATGGTGGTTCAAATGCTGGAAGAGGCAGAGCCTTATAAACTCACCTGCATAAGCACTGCAAAAGAAGTTGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

106.76

Weight (kDa)

6.1

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 193 - 323 1e-11 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 670 - 954 1.9e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 671 - 952 3.7e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2880
AarI CACCTGC 1 cut(s) 2897
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 2588, 2897
AccB1I GGYRCC 2 cut(s) 166, 1279
AccB7I CCANNNNNTGG 2 cut(s) 176, 1289
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 2 cut(s) 2046, 2700
AccII CGCG 1 cut(s) 2498
AciI CCGC 8 cut(s) 107, 195, 207, 923, 1013, 1064, 2363, 2803
AclWI GGATC 3 cut(s) 312, 325, 1588
AcsI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
AcuI CTGAAG 5 cut(s) 175, 585, 1386, 1839, 2088
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 6 cut(s) 332, 602, 1860, 1961, 2282, 2555
AfiI CCNNNNNNNGG 2 cut(s) 176, 1289
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 2 cut(s) 778, 980
AjiI CACGTC 1 cut(s) 1033
AjnI CCWGG 2 cut(s) 495, 509
Alw21I GWGCWC 3 cut(s) 957, 988, 2207
Alw26I GTCTC 5 cut(s) 10, 229, 458, 885, 2498
AlwI GGATC 3 cut(s) 312, 325, 1588
Ama87I CYCGRG 3 cut(s) 671, 743, 875
AoxI GGCC 3 cut(s) 217, 844, 1728
ApoI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
ArsI GACNNNNNNTTYG 6 cut(s) 527, 559, 863, 895, 1292, 1324
AsiGI ACCGGT 2 cut(s) 778, 980
Asp700I GAANNNNTTC 1 cut(s) 1118
AspS9I GGNCC 3 cut(s) 1271, 1468, 2097
AsuC2I CCSGG 5 cut(s) 475, 1068, 1758, 1828, 2644
AsuHPI GGTGA 9 cut(s) 970, 1355, 2014, 2126, 2239, 2359, 2387, 2632, 2878
AsuII TTCGAA 1 cut(s) 894
AvaI CYCGRG 3 cut(s) 671, 743, 875
AvaII GGWCC 3 cut(s) 1271, 1468, 2097
BaeGI GKGCMC 1 cut(s) 169
BamHI GGATCC 1 cut(s) 317
BanI GGYRCC 2 cut(s) 166, 1279
BanII GRGCYC 2 cut(s) 957, 2207
BauI CACGAG 2 cut(s) 364, 2746
BbsI GAAGAC 1 cut(s) 1644
Bbv12I GWGCWC 3 cut(s) 957, 988, 2207
BbvCI CCTCAGC 2 cut(s) 857, 1904
BceAI ACGGC 2 cut(s) 232, 1942
BcgI CGANNNNNNTGC 6 cut(s) 267, 301, 1622, 1656, 2488, 2522
BciT130I CCWGG 2 cut(s) 497, 511
BclI TGATCA 3 cut(s) 547, 1380, 2305
BcnI CCSGG 5 cut(s) 475, 1068, 1758, 1828, 2644
BcoDI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BfaI CTAG 2 cut(s) 1818, 2442
BfmI CTRYAG 2 cut(s) 1109, 2239
BfuAI ACCTGC 2 cut(s) 2588, 2897
BlpI GCTNAGC 2 cut(s) 1664, 1970
BmcAI AGTACT 2 cut(s) 1860, 2282
Bme1390I CCNGG 7 cut(s) 475, 497, 511, 1068, 1758, 1828, 2644
Bme18I GGWCC 3 cut(s) 1271, 1468, 2097
BmeT110I CYCGRG 3 cut(s) 671, 743, 875
BmgBI CACGTC 1 cut(s) 1033
BmgT120I GGNCC 3 cut(s) 1271, 1468, 2097
BmiI GGNNCC 5 cut(s) 168, 211, 319, 918, 1281
BmrFI CCNGG 7 cut(s) 475, 497, 511, 1068, 1758, 1828, 2644
BmsI GCATC 7 cut(s) 207, 627, 808, 1345, 2764, 2802, 2808
BpiI GAAGAC 1 cut(s) 1644
BplI GAGNNNNNCTC 2 cut(s) 2168, 2200
BpmI CTGGAG 2 cut(s) 518, 2547
Bpu10I CCTNAGC 2 cut(s) 857, 1904
Bpu1102I GCTNAGC 2 cut(s) 1664, 1970
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 2 cut(s) 104, 544
BpuMI CCSGG 5 cut(s) 475, 1068, 1758, 1828, 2644
Bsa29I ATCGAT 2 cut(s) 1095, 1333
BsaBI GATNNNNATC 1 cut(s) 1752
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 2498
BsaJI CCNNGG 5 cut(s) 485, 495, 529, 1826, 2398
BsaWI WCCGGW 3 cut(s) 767, 778, 980
BsaXI ACNNNNNCTCC 2 cut(s) 2336, 2366
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 1289
Bse118I RCCGGY 4 cut(s) 778, 980, 1620, 2821
Bse1I ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
Bse3DI GCAATG 3 cut(s) 1583, 1848, 2535
Bse8I GATNNNNATC 1 cut(s) 1752
BseBI CCWGG 2 cut(s) 497, 511
BseCI ATCGAT 2 cut(s) 1095, 1333
BseDI CCNNGG 5 cut(s) 485, 495, 529, 1826, 2398
BseGI GGATG 6 cut(s) 39, 83, 89, 1924, 2437, 2464
BseJI GATNNNNATC 1 cut(s) 1752
BseLI CCNNNNNNNGG 2 cut(s) 176, 1289
BseMI GCAATG 3 cut(s) 1583, 1848, 2535
BseNI ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
BseRI GAGGAG 2 cut(s) 1921, 1924
BseSI GKGCMC 1 cut(s) 169
BseYI CCCAGC 2 cut(s) 2540, 2694
BsgI GTGCAG 5 cut(s) 257, 346, 1786, 2297, 2791
Bsh1236I CGCG 1 cut(s) 2498
BshFI GGCC 3 cut(s) 219, 846, 1730
BshNI GGYRCC 2 cut(s) 166, 1279
BshTI ACCGGT 2 cut(s) 778, 980
BshVI ATCGAT 2 cut(s) 1095, 1333
BsiHKAI GWGCWC 3 cut(s) 957, 988, 2207
BsiHKCI CYCGRG 3 cut(s) 671, 743, 875
BslFI GGGAC 1 cut(s) 2557
BslI CCNNNNNNNGG 2 cut(s) 176, 1289
BsmAI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 2557
BsnI GGCC 3 cut(s) 219, 846, 1730
Bso31I GGTCTC 1 cut(s) 2498
BsoBI CYCGRG 3 cut(s) 671, 743, 875
Bsp119I TTCGAA 1 cut(s) 894
Bsp1286I GDGCHC 4 cut(s) 169, 957, 988, 2207
Bsp1720I GCTNAGC 2 cut(s) 1664, 1970
Bsp19I CCATGG 1 cut(s) 2398
Bsp68I TCGCGA 1 cut(s) 2498
BspACI CCGC 8 cut(s) 107, 195, 207, 923, 1013, 1064, 2363, 2803
BspANI GGCC 3 cut(s) 219, 846, 1730
BspDI ATCGAT 2 cut(s) 1095, 1333
BspFNI CGCG 1 cut(s) 2498
BspLI GGNNCC 5 cut(s) 168, 211, 319, 918, 1281
BspMAI CTGCAG 2 cut(s) 1113, 2243
BspMI ACCTGC 2 cut(s) 2588, 2897
BspPI GGATC 3 cut(s) 312, 325, 1588
BspQI GCTCTTC 4 cut(s) 62, 993, 1928, 2591
BspT104I TTCGAA 1 cut(s) 894
BspT107I GGYRCC 2 cut(s) 166, 1279
BspTNI GGTCTC 1 cut(s) 2498
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 3 cut(s) 1583, 1848, 2535
BsrFI RCCGGY 4 cut(s) 778, 980, 1620, 2821
BsrI ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
BssAI RCCGGY 4 cut(s) 778, 980, 1620, 2821
BssECI CCNNGG 5 cut(s) 485, 495, 529, 1826, 2398
BssNAI GTATAC 2 cut(s) 2047, 2701
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 2 cut(s) 364, 2746
BssT1I CCWWGG 1 cut(s) 2398
Bst1107I GTATAC 2 cut(s) 2047, 2701
Bst2BI CACGAG 2 cut(s) 364, 2746
Bst2UI CCWGG 2 cut(s) 497, 511
Bst4CI ACNGT 3 cut(s) 574, 693, 2261
Bst6I CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 276, 287, 1226, 2511
BstDSI CCRYGG 2 cut(s) 485, 2398
BstEII GGTNACC 1 cut(s) 2638
BstF5I GGATG 6 cut(s) 39, 83, 89, 1924, 2437, 2464
BstFNI CGCG 1 cut(s) 2498
BstMAI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BstNI CCWGG 2 cut(s) 497, 511
BstNSI RCATGY 1 cut(s) 2219
BstPI GGTNACC 1 cut(s) 2638
BstSCI CCNGG 7 cut(s) 473, 495, 509, 1066, 1756, 1826, 2642
BstSFI CTRYAG 2 cut(s) 1109, 2239
BstSLI GKGCMC 1 cut(s) 169
BstUI CGCG 1 cut(s) 2498
BstV2I GAAGAC 1 cut(s) 1644
BstX2I RGATCY 1 cut(s) 317
BstXI CCANNNNNNTGG 3 cut(s) 1412, 1697, 2845
BstYI RGATCY 1 cut(s) 317
BstZ17I GTATAC 2 cut(s) 2047, 2701
Bsu15I ATCGAT 2 cut(s) 1095, 1333
BsuRI GGCC 3 cut(s) 219, 846, 1730
BsuTUI ATCGAT 2 cut(s) 1095, 1333
BtgI CCRYGG 2 cut(s) 485, 2398
BtgZI GCGATG 4 cut(s) 52, 604, 2733, 2779
BtrI CACGTC 1 cut(s) 1033
BtsCI GGATG 6 cut(s) 39, 83, 89, 1924, 2437, 2464
BtsI GCAGTG 5 cut(s) 269, 2143, 2720, 2808, 2898
BtsIMutI CAGTG 6 cut(s) 269, 2143, 2198, 2720, 2808, 2898
BtuMI TCGCGA 1 cut(s) 2498
BveI ACCTGC 2 cut(s) 2588, 2897
Cac8I GCNNGC 4 cut(s) 276, 287, 1226, 2511
Cfr10I RCCGGY 4 cut(s) 778, 980, 1620, 2821
Cfr13I GGNCC 3 cut(s) 1271, 1468, 2097
ClaI ATCGAT 2 cut(s) 1095, 1333
CseI GACGC 1 cut(s) 169
CsiI ACCWGGT 1 cut(s) 509
Csp6I GTAC 6 cut(s) 331, 601, 1859, 1960, 2281, 2554
CspAI ACCGGT 2 cut(s) 778, 980
CviAII CATG 7 cut(s) 1512, 1777, 1944, 1964, 2216, 2399, 2839
CviQI GTAC 6 cut(s) 331, 601, 1859, 1960, 2281, 2554
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
EarI CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
EciI GGCGGA 1 cut(s) 938
Ecl136II GAGCTC 2 cut(s) 955, 2205
Eco130I CCWWGG 1 cut(s) 2398
Eco147I AGGCCT 1 cut(s) 1730
Eco24I GRGCYC 2 cut(s) 957, 2207
Eco31I GGTCTC 1 cut(s) 2498
Eco47I GGWCC 3 cut(s) 1271, 1468, 2097
Eco53kI GAGCTC 2 cut(s) 955, 2205
Eco57I CTGAAG 5 cut(s) 175, 585, 1386, 1839, 2088
Eco88I CYCGRG 3 cut(s) 671, 743, 875
Eco91I GGTNACC 1 cut(s) 2638
EcoICRI GAGCTC 2 cut(s) 955, 2205
EcoO109I RGGNCCY 1 cut(s) 1271
EcoO65I GGTNACC 1 cut(s) 2638
EcoRI GAATTC 2 cut(s) 1131, 1597
EcoRII CCWGG 2 cut(s) 495, 509
EcoT14I CCWWGG 1 cut(s) 2398
EcoT22I ATGCAT 1 cut(s) 2575
EcoT38I GRGCYC 2 cut(s) 957, 2207
ErhI CCWWGG 1 cut(s) 2398
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
FalI AAGNNNNNCTT 4 cut(s) 1976, 2008, 2125, 2157
FaqI GGGAC 1 cut(s) 2557
FatI CATG 7 cut(s) 1511, 1776, 1943, 1963, 2215, 2398, 2838
FauI CCCGC 1 cut(s) 1006
FbaI TGATCA 3 cut(s) 547, 1380, 2305
FblI GTMKAC 2 cut(s) 2046, 2700
FokI GGATG 6 cut(s) 26, 70, 76, 1911, 2444, 2471
FriOI GRGCYC 2 cut(s) 957, 2207
FspBI CTAG 2 cut(s) 1818, 2442
GsaI CCCAGC 2 cut(s) 2544, 2698
GsuI CTGGAG 2 cut(s) 518, 2547
HaeIII GGCC 3 cut(s) 219, 846, 1730
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 6 cut(s) 305, 587, 833, 1741, 1972, 2231
HpaI GTTAAC 1 cut(s) 516
HphI GGTGA 9 cut(s) 970, 1355, 2014, 2126, 2239, 2359, 2387, 2632, 2878
Hpy166II GTNNAC 7 cut(s) 402, 516, 1402, 2047, 2275, 2701, 2743
Hpy8I GTNNAC 7 cut(s) 402, 516, 1402, 2047, 2275, 2701, 2743
Hpy99I CGWCG 5 cut(s) 31, 34, 98, 218, 2606
HpyAV CCTTC 7 cut(s) 53, 833, 902, 1262, 1679, 1741, 1978
HpyCH4III ACNGT 3 cut(s) 574, 693, 2261
HpyCH4IV ACGT 2 cut(s) 1032, 2604
HpySE526I ACGT 2 cut(s) 1032, 2604
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
Ksp22I TGATCA 3 cut(s) 547, 1380, 2305
KspAI GTTAAC 1 cut(s) 516
LguI GCTCTTC 4 cut(s) 62, 993, 1928, 2591
LmnI GCTCC 6 cut(s) 215, 373, 1221, 1911, 2295, 2566
LweI GCATC 7 cut(s) 207, 627, 808, 1345, 2764, 2802, 2808
MabI ACCWGGT 1 cut(s) 509
MaeI CTAG 2 cut(s) 1818, 2442
MaeII ACGT 2 cut(s) 1032, 2604
MaeIII GTNAC 4 cut(s) 1076, 1308, 2114, 2638
MbiI CCGCTC 1 cut(s) 109
MfeI CAATTG 3 cut(s) 610, 1150, 1431
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 4 cut(s) 169, 957, 988, 2207
MlyI GAGTC 6 cut(s) 12, 460, 887, 1991, 2102, 2177
MmeI TCCRAC 8 cut(s) 50, 117, 237, 360, 763, 1017, 1653, 2364
Mph1103I ATGCAT 1 cut(s) 2575
MroXI GAANNNNTTC 1 cut(s) 1118
MseI TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
MslI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
MspA1I CMGCKG 3 cut(s) 195, 2311, 2805
MspR9I CCNGG 7 cut(s) 475, 497, 511, 1068, 1758, 1828, 2644
MunI CAATTG 3 cut(s) 610, 1150, 1431
MvaI CCWGG 2 cut(s) 497, 511
MvnI CGCG 1 cut(s) 2498
NciI CCSGG 5 cut(s) 475, 1068, 1758, 1828, 2644
NcoI CCATGG 1 cut(s) 2398
NlaIII CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
NlaIV GGNNCC 5 cut(s) 168, 211, 319, 918, 1281
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 3 cut(s) 1308, 2114, 2638
NruI TCGCGA 1 cut(s) 2498
NsiI ATGCAT 1 cut(s) 2575
NspI RCATGY 1 cut(s) 2219
NspV TTCGAA 1 cut(s) 894
PaeR7I CTCGAG 3 cut(s) 671, 743, 875
PaqCI CACCTGC 1 cut(s) 2897
PceI AGGCCT 1 cut(s) 1730
PciSI GCTCTTC 4 cut(s) 62, 993, 1928, 2591
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 669
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 7 cut(s) 642, 703, 786, 1171, 1258, 1499, 1704
PflFI GACNNNGTC 1 cut(s) 21
PflMI CCANNNNNTGG 2 cut(s) 176, 1289
PfoI TCCNGGA 1 cut(s) 473
PinAI ACCGGT 2 cut(s) 778, 980
PleI GAGTC 6 cut(s) 12, 459, 886, 1991, 2102, 2177
PpsI GAGTC 6 cut(s) 12, 459, 886, 1991, 2102, 2177
PpuMI RGGWCCY 1 cut(s) 1271
PsiI TTATAA 1 cut(s) 2880
Psp124BI GAGCTC 2 cut(s) 957, 2207
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 2 cut(s) 495, 509
PspEI GGTNACC 1 cut(s) 2638
PspFI CCCAGC 2 cut(s) 2540, 2694
PspGI CCWGG 2 cut(s) 495, 509
PspN4I GGNNCC 5 cut(s) 168, 211, 319, 918, 1281
PspPI GGNCC 3 cut(s) 1271, 1468, 2097
PspPPI RGGWCCY 1 cut(s) 1271
PspXI VCTCGAGB 3 cut(s) 671, 743, 875
PstI CTGCAG 2 cut(s) 1113, 2243
PsuI RGATCY 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 2311
RruI TCGCGA 1 cut(s) 2498
RsaI GTAC 6 cut(s) 332, 602, 1860, 1961, 2282, 2555
RsaNI GTAC 6 cut(s) 331, 601, 1859, 1960, 2281, 2554
RseI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SacI GAGCTC 2 cut(s) 957, 2207
SapI GCTCTTC 4 cut(s) 62, 993, 1928, 2591
SaqAI TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
Sau96I GGNCC 3 cut(s) 1271, 1468, 2097
ScaI AGTACT 2 cut(s) 1860, 2282
SchI GAGTC 6 cut(s) 12, 460, 887, 1991, 2102, 2177
ScrFI CCNGG 7 cut(s) 475, 497, 511, 1068, 1758, 1828, 2644
SduI GDGCHC 4 cut(s) 169, 957, 988, 2207
SexAI ACCWGGT 1 cut(s) 509
SfaNI GCATC 7 cut(s) 207, 627, 808, 1345, 2764, 2802, 2808
SfcI CTRYAG 2 cut(s) 1109, 2239
Sfr274I CTCGAG 3 cut(s) 671, 743, 875
SfuI TTCGAA 1 cut(s) 894
SgrAI CRCCGGYG 1 cut(s) 980
SinI GGWCC 3 cut(s) 1271, 1468, 2097
SlaI CTCGAG 3 cut(s) 671, 743, 875
SmiMI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SmlI CTYRAG 5 cut(s) 119, 523, 671, 743, 875
SmoI CTYRAG 5 cut(s) 119, 523, 671, 743, 875
SseBI AGGCCT 1 cut(s) 1730
SsiI CCGC 8 cut(s) 107, 195, 207, 923, 1013, 1064, 2363, 2803
SspI AATATT 1 cut(s) 2449
SspMI CTAG 2 cut(s) 1818, 2442
SstI GAGCTC 2 cut(s) 957, 2207
StuI AGGCCT 1 cut(s) 1730
StyD4I CCNGG 7 cut(s) 473, 495, 509, 1066, 1756, 1826, 2642
StyI CCWWGG 1 cut(s) 2398
TaaI ACNGT 3 cut(s) 574, 693, 2261
TaiI ACGT 2 cut(s) 1035, 2607
TaqI TCGA 8 cut(s) 663, 672, 744, 876, 894, 1095, 1333, 1702
TatI WGTACW 2 cut(s) 1858, 2280
TauI GCSGC 2 cut(s) 198, 210
TfiI GAWTC 7 cut(s) 642, 703, 786, 1171, 1258, 1499, 1704
Tru1I TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
Tru9I TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
TscAI CASTG 6 cut(s) 276, 2143, 2205, 2720, 2815, 2905
TseFI GTSAC 3 cut(s) 1308, 2114, 2638
Tsp45I GTSAC 3 cut(s) 1308, 2114, 2638
TspDTI ATGAA 8 cut(s) 55, 546, 1271, 1752, 1837, 1932, 1967, 2345
TspGWI ACGGA 4 cut(s) 11, 348, 359, 474
TspRI CASTG 6 cut(s) 276, 2143, 2205, 2720, 2815, 2905
Tth111I GACNNNGTC 1 cut(s) 21
Van91I CCANNNNNTGG 2 cut(s) 176, 1289
VpaK11BI GGWCC 3 cut(s) 1271, 1468, 2097
XapI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
XceI RCATGY 1 cut(s) 2219
XcmI CCANNNNNNNNNTGG 1 cut(s) 2845
XhoI CTCGAG 3 cut(s) 671, 743, 875
XmiI GTMKAC 2 cut(s) 2046, 2700
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 2 cut(s) 1818, 2442
ZrmI AGTACT 2 cut(s) 1860, 2282
Zsp2I ATGCAT 1 cut(s) 2575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.