MD10G1115100.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011518
Physical Location & Seq
Reverse (-)
19011837 .. 19015471
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1115100.v1.1.491

Sequence Viewer

Length: 2922 bp
ATGTCGTTCCGGCAGAGACTCCGTCCGACGACGACAACCATCCTCCTCCCTCTGCTCTTCCTCTTCATCTCCCTGTTTTCTCCATCCACATCCGACGAGCTTCAACCGCTCTTAAACCTCAAGCAATCCCTTGAAAACTCAAACCCAAGTCTTTTCACTTCTTGGGCACCACCCAATGGCGTCTGCAACTTCACCGGCATCGTCTGCAGCTCCGACGGCCTCGTCTCCCAAATCAACCTCTCGCAGCAAAACCTTTCTGGGTTTCTTCCACTGCACGCAATCTGCTCGCTTCCATCGTTAAAAAAGCTTTCTTTGGGATCCAATGGCTTGTACGGAAGCCTCACGGACGACCTGAAAAACTGCACGAGCTTGGAGCAGTTGGATTTGGGTAAAAATTCGTTTACTGGAAAAGTTCCTGACTTGTCTGCTTTAACAAAATTAACCCTCCTGAGTCTCAATGGCAGCGGATTTTCCGGGAAATTTCCGTGGAAATCCCTGGAGAACCTGACCAGGTTAACTTTCTTGAGCCTCGGCGACAACCCTTTTGATCAAACTTCATTTCCTGAAGAAACTGTGAGGCTTGATAAGCTTTACTGGCTGTACCTCTCCAATTGCAGCATCGCCGGAAAAATCCCAGATGGAATCGGAAACCTCACTCTGCTCGAAAACCTCGAGATTTCGGACAACCAACTGTCCGGAGAGATTCCCCAGAGAATTTCAAATCTCAGGAAGCTATGGCAGCTCGAGCTTTACCGAAACTTTCTGACCGGACAAATACCGGTTGGATTCGGCAACCTCACAAGCCTTGTCAATTTTGATGCCTCAGCTAACAAGCTTGAAGGTGGCCTCTCTGAGCTGAGGTTCTTGACAAAGCTCGAGTCTCTGCATTTGTTCGAAAACCGATTAGAAGGCGAGGTTCCGGCGGAGTTTGGGGAGTTGAAGAACCTTGCCAAGCTCTCACTTTACAGGAACAAGCTCGCCGGTGCTCTTCCTCAAAAGCTCGGTTCTTGGGCGGGATTGGATTACATTGACGTGTCGGAGAACTATCTGACTGGTGCTATACCGCCCGGAATGTGTAGCAATGGGAAAATGATTGATTTTCTCCTGCTGCAGAACAATTTCACAGGTGGAATTCCTGACAGCTATGCCAATTGTAAGTCTTTGGTTCGGATTCGTGTGAGTAAAAATTCGCTTTCGGGTTCTATCCCTGCTGGATTATGGAGCTTGCCAAATGTTGTTGTCCTTGATCTTTCTATGAATCAGTTTGAAGGTCCTCTGGCACCAGAAATTGGTAAGGCAAACTCTCTGTCACTATTGCTTTTAGCGAATAATCGATTTTCGGGTGAATTGCCAGATGCTTTGTCTGAAGCTACCTCATTGATCACAATTCAGATGAGTGTGAACCAATTTAGTGGGAAAATCCCAGAAACAATTGGGAGTTTGAAGAAATTAAGCAGTCTTGATTTGGACCAAAATAAGCTGTCTGGTACTATGCCGGAATCATTAGGCTCATGTTTTGGTATTTCTGAAGTAAACCTTGCCCACAACTATATTTCTGGCGAAATCCCATCAAGTCTTGGATCATTGCATAATCTGAATTCCTTGAACTTATCTGGAAACCGGCTTTCCAACGAAATTCCTGCTACTTTGTCGTCCCTAAAACTCAGCCTTCTTGATCTGACAAATAACCAGTTGATTGGTCGAATCCCCGAAGCATTGTCAATTCGGGCCTTCAATGAAAGCTTTGATGGAAATCCGGGTTTGTGCAGTCAGAACATGGAAAATGTGAGGTCTTGTCCTTTCAAGTCTGGCACTTCTAGCCGACCCCGGATATTCTTGTCCAGTTTCATTGCTTCAGTACTTGTCGTGCTTGTTTTGCTCGGTTTGTTCTTGCTCTTGAAGCTGAGGAGGAGCAATCTTGACCATTCATTGAAGAGCAATTCATGGACTATGAAGCCGTACCATGTGCTAAGCTTTACAGAGAAGGAAATCTTAGACTCGGTGAAAGCTGAGAACTTAATTGGCAAAGGAGGGTCAGGGAATGTATACAAAGTTGTATTGAGTGATGGGAAAGAACTCGCTGTAAAGCACATTTGGACCACTTCAGACTCAGGTGACAGAAAAAGCTACCGAAGCAGTGCTTCCATCTTGAAGAAGTCTAAATCGAGGTCATCAGAGTATGACTCAGAAGTGGCTACACTGAGCTCTCTGAGGCATGTGAATGTGGTGAAGCTTTACTGCAGCATATCAAGTGAGGATAGTAATCTTCTGGTTTACGAGTACTTTCCTAACGGGAGCTTATATGATCAGCTGCACACAAGTAAGAAGATGAAGATGGGGTGGGAGGTGAGGTATGAGATTGCGGTGGGGGCTGCGAGGGGGTTGGAGTATCTTCACCATGGTAGCAGTCATAGACCTGTGATACACAGGGATGTGAAGTCTAGTAATATTCTGCTGGATGGAGATTGGAAGCCAAGAATTGCTGATTTTGGTCTCGCAAAGATTATGCAGGCTGGTGTAGATTGCACTCAGGTCATTGCCGGGACACTTGGATACATAGCTCCAGAGTATGCATATACCTGCAAGATCAATGAGAAGAGTGACGTTTATAGCTTCGGGGTGGTGTTGATGGAGTTGGTCACCGGGAAGGAGCCGGTTGAGGCAGCGTTCGGAGAGAACAATGATATAGTGAGCTGGGTATACAGCAAAATGCAGTGCGATGATAGTGTGCTTGAATTGGTGGACACGAGCATTTCTGACGATCTCAAAGAAGATGCCATCAATGTTTTGAGCATCGCAGTCCGCTGCACTGCCAAGGTGCCGGTTCTAAGACCCTCCATGAGAATGGTGGTTCAAATGCTGGAAGAGGCAGAGCCTTATAAACTCACCTGCATAAGCACTGCAAAAGAAGTTGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

106.63

Weight (kDa)

6.1

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 70 4.5e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 193 - 322 3.3e-12 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 670 - 954 2.2e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 671 - 952 2.2e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2880
AarI CACCTGC 1 cut(s) 2897
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 2588, 2897
AccB1I GGYRCC 3 cut(s) 166, 1279, 2818
AccB7I CCANNNNNTGG 2 cut(s) 176, 1289
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 2 cut(s) 2046, 2700
AccIII TCCGGA 1 cut(s) 695
AciI CCGC 7 cut(s) 107, 465, 923, 1013, 1064, 2363, 2803
AclWI GGATC 3 cut(s) 312, 325, 1588
AcsI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
AcuI CTGAAG 5 cut(s) 585, 1386, 1548, 1839, 2088
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 6 cut(s) 332, 602, 1489, 1860, 1961, 2282
AfiI CCNNNNNNNGG 2 cut(s) 176, 1289
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 1 cut(s) 778
AjiI CACGTC 1 cut(s) 1033
AjnI CCWGG 2 cut(s) 495, 509
Alw21I GWGCWC 2 cut(s) 988, 2207
Alw26I GTCTC 5 cut(s) 10, 229, 458, 885, 2498
AlwI GGATC 3 cut(s) 312, 325, 1588
Ama87I CYCGRG 3 cut(s) 671, 743, 875
Aor13HI TCCGGA 1 cut(s) 695
AoxI GGCC 3 cut(s) 217, 844, 1728
ApoI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
ArsI GACNNNNNNTTYG 6 cut(s) 527, 559, 863, 895, 1292, 1324
AsiGI ACCGGT 1 cut(s) 778
Asp700I GAANNNNTTC 1 cut(s) 1118
AspS9I GGNCC 4 cut(s) 1271, 1468, 1728, 2097
AsuC2I CCSGG 6 cut(s) 475, 1068, 1758, 1828, 2542, 2644
AsuHPI GGTGA 9 cut(s) 184, 1355, 2014, 2126, 2239, 2359, 2387, 2632, 2878
AsuII TTCGAA 1 cut(s) 894
AvaI CYCGRG 3 cut(s) 671, 743, 875
AvaII GGWCC 3 cut(s) 1271, 1468, 2097
BaeGI GKGCMC 1 cut(s) 169
BamHI GGATCC 1 cut(s) 317
BanI GGYRCC 3 cut(s) 166, 1279, 2818
BanII GRGCYC 1 cut(s) 2207
BauI CACGAG 2 cut(s) 364, 2746
Bbv12I GWGCWC 2 cut(s) 988, 2207
BbvCI CCTCAGC 3 cut(s) 823, 857, 1904
BceAI ACGGC 2 cut(s) 232, 1942
BcgI CGANNNNNNTGC 4 cut(s) 267, 301, 1622, 1656
BciT130I CCWGG 2 cut(s) 497, 511
BciVI GTATCC 1 cut(s) 2546
BclI TGATCA 3 cut(s) 547, 1380, 2305
BcnI CCSGG 6 cut(s) 475, 1068, 1758, 1828, 2542, 2644
BcoDI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BfaI CTAG 2 cut(s) 1818, 2442
BfmI CTRYAG 3 cut(s) 205, 1109, 2239
BfuAI ACCTGC 2 cut(s) 2588, 2897
BfuI GTATCC 1 cut(s) 2546
BlpI GCTNAGC 1 cut(s) 1970
BmcAI AGTACT 2 cut(s) 1860, 2282
Bme1390I CCNGG 8 cut(s) 475, 497, 511, 1068, 1758, 1828, 2542, 2644
Bme18I GGWCC 3 cut(s) 1271, 1468, 2097
BmeT110I CYCGRG 3 cut(s) 671, 743, 875
BmgBI CACGTC 1 cut(s) 1033
BmgT120I GGNCC 4 cut(s) 1271, 1468, 1728, 2097
BmiI GGNNCC 6 cut(s) 168, 319, 918, 1281, 2652, 2820
BmrFI CCNGG 8 cut(s) 475, 497, 511, 1068, 1758, 1828, 2542, 2644
BmsI GCATC 6 cut(s) 207, 627, 808, 1345, 2764, 2802
BplI GAGNNNNNCTC 2 cut(s) 2168, 2200
BpmI CTGGAG 2 cut(s) 518, 2547
Bpu10I CCTNAGC 3 cut(s) 823, 857, 1904
Bpu1102I GCTNAGC 1 cut(s) 1970
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 2 cut(s) 104, 544
BpuMI CCSGG 6 cut(s) 475, 1068, 1758, 1828, 2542, 2644
Bsa29I ATCGAT 1 cut(s) 1333
BsaBI GATNNNNATC 2 cut(s) 1752, 2262
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 2498
BsaJI CCNNGG 6 cut(s) 485, 495, 529, 1826, 2398, 2814
BsaWI WCCGGW 3 cut(s) 695, 767, 778
BsaXI ACNNNNNCTCC 2 cut(s) 2336, 2366
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 1289
Bse118I RCCGGY 6 cut(s) 194, 778, 980, 1620, 2653, 2821
Bse1I ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
Bse3DI GCAATG 4 cut(s) 1087, 1583, 1848, 2535
Bse8I GATNNNNATC 2 cut(s) 1752, 2262
BseAI TCCGGA 1 cut(s) 695
BseBI CCWGG 2 cut(s) 497, 511
BseCI ATCGAT 1 cut(s) 1333
BseDI CCNNGG 6 cut(s) 485, 495, 529, 1826, 2398, 2814
BseGI GGATG 5 cut(s) 39, 83, 89, 2437, 2464
BseJI GATNNNNATC 2 cut(s) 1752, 2262
BseLI CCNNNNNNNGG 2 cut(s) 176, 1289
BseMI GCAATG 4 cut(s) 1087, 1583, 1848, 2535
BseNI ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
BseRI GAGGAG 3 cut(s) 35, 1921, 1924
BseSI GKGCMC 1 cut(s) 169
BseYI CCCAGC 1 cut(s) 2694
BsgI GTGCAG 5 cut(s) 257, 346, 1786, 2297, 2791
BshFI GGCC 3 cut(s) 219, 846, 1730
BshNI GGYRCC 3 cut(s) 166, 1279, 2818
BshTI ACCGGT 1 cut(s) 778
BshVI ATCGAT 1 cut(s) 1333
BsiHKAI GWGCWC 2 cut(s) 988, 2207
BsiHKCI CYCGRG 3 cut(s) 671, 743, 875
BslFI GGGAC 2 cut(s) 1639, 2557
BslI CCNNNNNNNGG 2 cut(s) 176, 1289
BsmAI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 2 cut(s) 1639, 2557
BsnI GGCC 3 cut(s) 219, 846, 1730
Bso31I GGTCTC 1 cut(s) 2498
BsoBI CYCGRG 3 cut(s) 671, 743, 875
Bsp119I TTCGAA 1 cut(s) 894
Bsp1286I GDGCHC 3 cut(s) 169, 988, 2207
Bsp13I TCCGGA 1 cut(s) 695
Bsp143I GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
Bsp1720I GCTNAGC 1 cut(s) 1970
Bsp19I CCATGG 1 cut(s) 2398
BspACI CCGC 7 cut(s) 107, 465, 923, 1013, 1064, 2363, 2803
BspANI GGCC 3 cut(s) 219, 846, 1730
BspDI ATCGAT 1 cut(s) 1333
BspEI TCCGGA 1 cut(s) 695
BspLI GGNNCC 6 cut(s) 168, 319, 918, 1281, 2652, 2820
BspMAI CTGCAG 3 cut(s) 209, 1113, 2243
BspMI ACCTGC 2 cut(s) 2588, 2897
BspPI GGATC 3 cut(s) 312, 325, 1588
BspQI GCTCTTC 3 cut(s) 62, 993, 1928
BspT104I TTCGAA 1 cut(s) 894
BspT107I GGYRCC 3 cut(s) 166, 1279, 2818
BspTNI GGTCTC 1 cut(s) 2498
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 4 cut(s) 1087, 1583, 1848, 2535
BsrFI RCCGGY 6 cut(s) 194, 778, 980, 1620, 2653, 2821
BsrI ACTGG 5 cut(s) 409, 599, 1057, 1690, 1842
BssAI RCCGGY 6 cut(s) 194, 778, 980, 1620, 2653, 2821
BssECI CCNNGG 6 cut(s) 485, 495, 529, 1826, 2398, 2814
BssMI GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
BssNAI GTATAC 2 cut(s) 2047, 2701
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 2 cut(s) 364, 2746
BssT1I CCWWGG 2 cut(s) 2398, 2814
Bst1107I GTATAC 2 cut(s) 2047, 2701
Bst2BI CACGAG 2 cut(s) 364, 2746
Bst2UI CCWGG 2 cut(s) 497, 511
Bst4CI ACNGT 2 cut(s) 574, 693
Bst6I CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 5 cut(s) 276, 287, 978, 1226, 2511
BstDSI CCRYGG 2 cut(s) 485, 2398
BstEII GGTNACC 1 cut(s) 2638
BstF5I GGATG 5 cut(s) 39, 83, 89, 2437, 2464
BstKTI GATC 9 cut(s) 320, 550, 1249, 1383, 1583, 1678, 2308, 2589, 2764
BstMAI GTCTC 5 cut(s) 10, 229, 458, 885, 2498
BstMBI GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
BstNI CCWGG 2 cut(s) 497, 511
BstNSI RCATGY 1 cut(s) 2219
BstPI GGTNACC 1 cut(s) 2638
BstSCI CCNGG 8 cut(s) 473, 495, 509, 1066, 1756, 1826, 2540, 2642
BstSFI CTRYAG 3 cut(s) 205, 1109, 2239
BstSLI GKGCMC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 317
BstXI CCANNNNNNTGG 3 cut(s) 1412, 1697, 2845
BstYI RGATCY 1 cut(s) 317
BstZ17I GTATAC 2 cut(s) 2047, 2701
Bsu15I ATCGAT 1 cut(s) 1333
BsuI GTATCC 1 cut(s) 2546
BsuRI GGCC 3 cut(s) 219, 846, 1730
BsuTUI ATCGAT 1 cut(s) 1333
BtgI CCRYGG 2 cut(s) 485, 2398
BtgZI GCGATG 3 cut(s) 604, 2733, 2779
BtrI CACGTC 1 cut(s) 1033
BtsCI GGATG 5 cut(s) 39, 83, 89, 2437, 2464
BtsI GCAGTG 5 cut(s) 269, 2143, 2720, 2808, 2898
BtsIMutI CAGTG 6 cut(s) 269, 2143, 2198, 2720, 2808, 2898
BveI ACCTGC 2 cut(s) 2588, 2897
Cac8I GCNNGC 5 cut(s) 276, 287, 978, 1226, 2511
Cfr10I RCCGGY 6 cut(s) 194, 778, 980, 1620, 2653, 2821
Cfr13I GGNCC 4 cut(s) 1271, 1468, 1728, 2097
ClaI ATCGAT 1 cut(s) 1333
CseI GACGC 1 cut(s) 169
CsiI ACCWGGT 1 cut(s) 509
Csp6I GTAC 6 cut(s) 331, 601, 1488, 1859, 1960, 2281
CspAI ACCGGT 1 cut(s) 778
CviAII CATG 7 cut(s) 1512, 1777, 1944, 1964, 2216, 2399, 2839
CviQI GTAC 6 cut(s) 331, 601, 1488, 1859, 1960, 2281
DpnI GATC 9 cut(s) 319, 549, 1248, 1382, 1582, 1677, 2307, 2588, 2763
DpnII GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
EarI CTCTTC 6 cut(s) 62, 68, 993, 1928, 2591, 2859
EciI GGCGGA 1 cut(s) 938
Ecl136II GAGCTC 1 cut(s) 2205
Eco130I CCWWGG 2 cut(s) 2398, 2814
Eco24I GRGCYC 1 cut(s) 2207
Eco31I GGTCTC 1 cut(s) 2498
Eco47I GGWCC 3 cut(s) 1271, 1468, 2097
Eco53kI GAGCTC 1 cut(s) 2205
Eco57I CTGAAG 5 cut(s) 585, 1386, 1548, 1839, 2088
Eco88I CYCGRG 3 cut(s) 671, 743, 875
Eco91I GGTNACC 1 cut(s) 2638
EcoICRI GAGCTC 1 cut(s) 2205
EcoO109I RGGNCCY 1 cut(s) 1271
EcoO65I GGTNACC 1 cut(s) 2638
EcoRI GAATTC 2 cut(s) 1131, 1597
EcoRII CCWGG 2 cut(s) 495, 509
EcoT14I CCWWGG 2 cut(s) 2398, 2814
EcoT22I ATGCAT 1 cut(s) 2575
EcoT38I GRGCYC 1 cut(s) 2207
ErhI CCWWGG 2 cut(s) 2398, 2814
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
FalI AAGNNNNNCTT 6 cut(s) 1521, 1553, 1976, 2008, 2125, 2157
FaqI GGGAC 2 cut(s) 1639, 2557
FatI CATG 7 cut(s) 1511, 1776, 1943, 1963, 2215, 2398, 2838
FauI CCCGC 1 cut(s) 1006
FbaI TGATCA 3 cut(s) 547, 1380, 2305
FblI GTMKAC 2 cut(s) 2046, 2700
FokI GGATG 5 cut(s) 26, 70, 76, 2444, 2471
FriOI GRGCYC 1 cut(s) 2207
FspBI CTAG 2 cut(s) 1818, 2442
GsaI CCCAGC 1 cut(s) 2698
GsuI CTGGAG 2 cut(s) 518, 2547
HaeIII GGCC 3 cut(s) 219, 846, 1730
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 6 cut(s) 305, 587, 833, 1741, 1972, 2231
HpaI GTTAAC 1 cut(s) 516
HphI GGTGA 9 cut(s) 184, 1355, 2014, 2126, 2239, 2359, 2387, 2632, 2878
Hpy166II GTNNAC 8 cut(s) 402, 516, 1402, 1534, 2047, 2275, 2701, 2743
Hpy8I GTNNAC 8 cut(s) 402, 516, 1402, 1534, 2047, 2275, 2701, 2743
Hpy99I CGWCG 4 cut(s) 31, 34, 98, 218
HpyAV CCTTC 7 cut(s) 833, 902, 1262, 1679, 1741, 1978, 2641
HpyCH4III ACNGT 2 cut(s) 574, 693
HpyCH4IV ACGT 2 cut(s) 1032, 2604
HpySE526I ACGT 2 cut(s) 1032, 2604
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
Kpn2I TCCGGA 1 cut(s) 695
Ksp22I TGATCA 3 cut(s) 547, 1380, 2305
KspAI GTTAAC 1 cut(s) 516
Kzo9I GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
LguI GCTCTTC 3 cut(s) 62, 993, 1928
LmnI GCTCC 7 cut(s) 215, 373, 1221, 1911, 2295, 2566, 2650
LweI GCATC 6 cut(s) 207, 627, 808, 1345, 2764, 2802
MabI ACCWGGT 1 cut(s) 509
MaeI CTAG 2 cut(s) 1818, 2442
MaeII ACGT 2 cut(s) 1032, 2604
MaeIII GTNAC 4 cut(s) 1308, 2114, 2600, 2638
MalI GATC 9 cut(s) 319, 549, 1248, 1382, 1582, 1677, 2307, 2588, 2763
MbiI CCGCTC 1 cut(s) 109
MboI GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
MfeI CAATTG 3 cut(s) 610, 1150, 1431
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 3 cut(s) 169, 988, 2207
MlyI GAGTC 6 cut(s) 12, 460, 887, 1991, 2102, 2177
MmeI TCCRAC 8 cut(s) 50, 117, 237, 360, 763, 1017, 1653, 2364
Mph1103I ATGCAT 1 cut(s) 2575
MroI TCCGGA 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 1118
MseI TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
MslI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
MspA1I CMGCKG 3 cut(s) 465, 2311, 2805
MspR9I CCNGG 8 cut(s) 475, 497, 511, 1068, 1758, 1828, 2542, 2644
MunI CAATTG 3 cut(s) 610, 1150, 1431
MvaI CCWGG 2 cut(s) 497, 511
NciI CCSGG 6 cut(s) 475, 1068, 1758, 1828, 2542, 2644
NcoI CCATGG 1 cut(s) 2398
NdeII GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
NlaIII CATG 7 cut(s) 1515, 1780, 1947, 1967, 2219, 2402, 2842
NlaIV GGNNCC 6 cut(s) 168, 319, 918, 1281, 2652, 2820
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 4 cut(s) 1308, 2114, 2600, 2638
NsiI ATGCAT 1 cut(s) 2575
NspI RCATGY 1 cut(s) 2219
NspV TTCGAA 1 cut(s) 894
PaeR7I CTCGAG 3 cut(s) 671, 743, 875
PaqCI CACCTGC 1 cut(s) 2897
PciSI GCTCTTC 3 cut(s) 62, 993, 1928
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 669
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 7 cut(s) 642, 703, 786, 1171, 1258, 1499, 1704
PflFI GACNNNGTC 1 cut(s) 21
PflMI CCANNNNNTGG 2 cut(s) 176, 1289
PfoI TCCNGGA 1 cut(s) 473
PinAI ACCGGT 1 cut(s) 778
PleI GAGTC 6 cut(s) 12, 459, 886, 1991, 2102, 2177
PpsI GAGTC 6 cut(s) 12, 459, 886, 1991, 2102, 2177
PpuMI RGGWCCY 1 cut(s) 1271
PsiI TTATAA 1 cut(s) 2880
Psp124BI GAGCTC 1 cut(s) 2207
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 2 cut(s) 495, 509
PspEI GGTNACC 1 cut(s) 2638
PspFI CCCAGC 1 cut(s) 2694
PspGI CCWGG 2 cut(s) 495, 509
PspN4I GGNNCC 6 cut(s) 168, 319, 918, 1281, 2652, 2820
PspPI GGNCC 4 cut(s) 1271, 1468, 1728, 2097
PspPPI RGGWCCY 1 cut(s) 1271
PspXI VCTCGAGB 2 cut(s) 743, 875
PstI CTGCAG 3 cut(s) 209, 1113, 2243
PsuI RGATCY 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 2311
RsaI GTAC 6 cut(s) 332, 602, 1489, 1860, 1961, 2282
RsaNI GTAC 6 cut(s) 331, 601, 1488, 1859, 1960, 2281
RseI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SacI GAGCTC 1 cut(s) 2207
SapI GCTCTTC 3 cut(s) 62, 993, 1928
SaqAI TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
Sau3AI GATC 9 cut(s) 317, 547, 1246, 1380, 1580, 1675, 2305, 2586, 2761
Sau96I GGNCC 4 cut(s) 1271, 1468, 1728, 2097
ScaI AGTACT 2 cut(s) 1860, 2282
SchI GAGTC 6 cut(s) 12, 460, 887, 1991, 2102, 2177
ScrFI CCNGG 8 cut(s) 475, 497, 511, 1068, 1758, 1828, 2542, 2644
SduI GDGCHC 3 cut(s) 169, 988, 2207
SexAI ACCWGGT 1 cut(s) 509
SfaNI GCATC 6 cut(s) 207, 627, 808, 1345, 2764, 2802
SfcI CTRYAG 3 cut(s) 205, 1109, 2239
Sfr274I CTCGAG 3 cut(s) 671, 743, 875
SfuI TTCGAA 1 cut(s) 894
SgrAI CRCCGGYG 1 cut(s) 980
SinI GGWCC 3 cut(s) 1271, 1468, 2097
SlaI CTCGAG 3 cut(s) 671, 743, 875
SmiMI CAYNNNNRTG 4 cut(s) 1031, 2220, 2430, 2843
SmlI CTYRAG 5 cut(s) 119, 523, 671, 743, 875
SmoI CTYRAG 5 cut(s) 119, 523, 671, 743, 875
SsiI CCGC 7 cut(s) 107, 465, 923, 1013, 1064, 2363, 2803
SspI AATATT 1 cut(s) 2449
SspMI CTAG 2 cut(s) 1818, 2442
SstI GAGCTC 1 cut(s) 2207
StyD4I CCNGG 8 cut(s) 473, 495, 509, 1066, 1756, 1826, 2540, 2642
StyI CCWWGG 2 cut(s) 2398, 2814
TaaI ACNGT 2 cut(s) 574, 693
TaiI ACGT 2 cut(s) 1035, 2607
TaqI TCGA 8 cut(s) 663, 672, 744, 876, 894, 1333, 1702, 2165
TatI WGTACW 2 cut(s) 1858, 2280
TfiI GAWTC 7 cut(s) 642, 703, 786, 1171, 1258, 1499, 1704
Tru1I TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
Tru9I TTAA 7 cut(s) 113, 299, 431, 440, 515, 1451, 2018
TscAI CASTG 6 cut(s) 276, 2143, 2205, 2720, 2815, 2905
TseFI GTSAC 4 cut(s) 1308, 2114, 2600, 2638
Tsp45I GTSAC 4 cut(s) 1308, 2114, 2600, 2638
TspDTI ATGAA 9 cut(s) 55, 546, 1271, 1752, 1837, 1917, 1932, 1967, 2345
TspGWI ACGGA 4 cut(s) 11, 348, 359, 474
TspRI CASTG 6 cut(s) 276, 2143, 2205, 2720, 2815, 2905
Tth111I GACNNNGTC 1 cut(s) 21
Van91I CCANNNNNTGG 2 cut(s) 176, 1289
VpaK11BI GGWCC 3 cut(s) 1271, 1468, 2097
XapI RAATTY 7 cut(s) 394, 479, 714, 1131, 1186, 1597, 1635
XceI RCATGY 1 cut(s) 2219
XcmI CCANNNNNNNNNTGG 1 cut(s) 2845
XhoI CTCGAG 3 cut(s) 671, 743, 875
XmiI GTMKAC 2 cut(s) 2046, 2700
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 2 cut(s) 1818, 2442
ZrmI AGTACT 2 cut(s) 1860, 2282
Zsp2I ATGCAT 1 cut(s) 2575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.