Rroxscaffold_7G00214660

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011518
Physical Location & Seq
Reverse (-)
65308158 .. 65311722
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214660.1

Sequence Viewer

Length: 2946 bp
ATGTCGTTCATCCGGCAACGGCTCCCTCCGGTTCTTCTTCTTCTCCTCTCTCTCCTCTTCCTCTCCTTACTCTCTCCCTCCACATCAACCGAGCTCGACCAACTCTTGAAGCTCAAAACCTCACTTGAAAGTTCAAACCCAACTCTGTTCAGTTCTTGGAAGCAAGAAAATCCCACGTGCAAATTCACTGGGATTGTCTGCAACTCAAATGGACTGGTCTCAGAAATCAATCTTTCTCAGCGAAACCTTGCTGGCTCTCTTCCTTTCGACGCAATATGCTCGCTTCCACAACTAGAAAAGCTTTCTTTTGGTTCAAATTTCCTGCACGGAAGTCTCACTGAGGATTTGAAGAAGTGTACTAATTTGCAGGAATTGGATTTGGCTAAGAATTCTTTCACAGGAAAAGTACCTGACTTGGGTTCTTTGAGTCAATTGAGGCTGTTGAGTCTGAATGCTAGTGGGGTGTCTGGACTTTTTCCATGGAAATCACTGGAGAATCTGACCGAGTTAGCTTTTCTGAGCCTTGGTGATAACAGATTCGATGCAAGTCCTATTGCAATGGAGTTGGGAAAGTTTGACAAGCTTTACTGGCTTTACCTCTCAAATTGTAGCATCACAGGTGAAATCCCAGAGGGTATTGGAAACCTGACTCTGCTTGAGAATCTGGAGCTTTCGGATAACCAATTGGTTGGTGAAATTCCTCAGAGTATTACAAATCTCAAGAAGCTCAGGCAACTTGAGATTTATGCTAATTTCCTGACTGGGAAAATCCCGGTTGGGATTGGCAACCTCACAAGCCTTAGGAATTTTGATGTTTCAACTAACAAGCTTGAGGGTGATCTCTCTGAGCTCAAATCCTTGACAGGGCTTGAGTCTGTGCAGCTTTATGAGAATCACTTAGTTGGCGAAGTTCCGGAGGAGTTTGGAGAGTTCAAGAACCTTTATCAGCTCTCACTTTACAAGAACAAGCTCACTGGCCCTCTTCCTCAGAAGCTTGGTTCTTGGTCTGGCATGGAGTACATTGATGTTTCAGAGAACTACTTGACAGGCCCTATACCCCCTGGCATGTGCAAAAATGAGAAACTGACTGATTTTCTGCTGCTGCAGAACAATTTCACAGGTGGGATTTCTGAGTCCTATGCAAATTGCAAGTCTTTAGTTCGGTTACGTGTGAATAACAATTCGCTTTCGGGTACTGTCCCTGTTGGAATTTGGAGCTTGCCAAGTGTTGTAGTTATGGATCTTTCTATGAATCAGTTTGAAGGTCCTCTGACTTCAGATGTTGGTAAGGCAAATTCTCTGTCATTATTACTCTTAGCTAATAATCGATTTTCGGGTGAATTACCTGCTGCTTTGTCTGAAGCTACCTCATTGGTAACGATTCAGCTTAGTATGAACCAATTTGAGGGTCAAATCCCAGCAACAATTGGGAACTTGAAGAAATTAGGCAGTTTTCATTTAGAACAAAATTTGTTGTCTGGTGCTATACCGGACTCAATAGGCTCATGTGTTAGGATTTCTGAAATCAACCTTGCCCATAACAATCTTTCTGGCCAAATCCCTCCAAGTCTTGGATCATTGCCTAACTTAAATTCCTTGAACTTATCCGGAAACCAACTTTCAAGTGAAGTTCCTGCTACTTTGTCATCCCTCAAACTCAGCCTTCTTGATCTTACAAACAACCAGTTGATTGGCCGCGTGCCTGAGTCACTATCCATCGCAGCTTTTAATGAAAGCTTTGATGGAAATCCTGGTTTGTGCAGCCAGACCATGAAGAATTTGCGGCCTTGTTCATCCAATTCGGGCTCTCCTAGCCGCCACCCACGGATATTTTTGTCTTCTTTTATTGCTGGAGTACTTGTTCTGCTTATTTTGCTTGGTGTGTTCTTGCTTTGGAAGCTAAGGAGGAGCAGTCTTGATCAACCATTGAAGAGTAATTCATGGACTCTGAAGCAGTACCATGTGCTTACCTTCACAGAGAATGAAATCCTAGATTCAATCAAAGCTGAAAATTTGATTGGCAAAGGAGGGTCAGGAAATGTATACAAAGTACAACTGAGAGATGGGAAAGCACTTGCTGTGAAGCACATTTGGACTTCACCAGACTCCGCTTACCAGAAAACTTATCGAAGCACCACTTCCATTTTGAAGAAGTCTAAATCCAGCTCATCGGAATATGATGCCGAGGTGGCCACACTGAGCTCATTGAGGCATGTGAATGTGGTGAAGCTATACTGCAGCATAAGTAGTGAGGACAGCAATCTGCTGGTTTACGAGTACTTTCCCAATGGGAGCTTATGGGATCAGCTGCACATGAATAACAAGGTCAAGATGGGGTGGGAAGTGAGGTATGAGATTGCTTTGGGGGCTGCAAGAGGATTGGAGTATCTTCACCATGGTAGTCACAGACCTGTTATACACAGAGATGTGAAGTCCAGTAATATTCTGCTAGATGGAGATTGGAAGCCTAGAATTGCTGATTTTGGGCTTGCCAAGATTGTGCAGGCTGGTGGTGAAATGACTCATGTCATTGCTGGGACACTCGGCTACATAGCTCCTGAGTATGCATATACCTACAAGGTAAATGAGAAGAGTGATGTGTATAGCTTTGGGGTGGTCTTGATGGAGTTGGTGACAGGAAAGAGGCCGGTGGAGACTGATTTTGGAGAGAACAAGGACATAGTGAGCTGGGTTTGCAGCAAAATGCAGTGCGGAGAGAGTGAGCTTGAGTTGGTGGACTCAAGCATTGACGATGCTCTACAAGAAGATGCCCTCAACGTTTTGAGCATTGCAATTCGCTGCACCGCTAGGGTTCCGGTTCTAAGACCCTCGATGAGAATGGTGGTTCAAATGCTGGAAGAAGCACAACCTTGTCAACTCACCAGCATAAGCATCACCAAGGATGGTGAAAATAACCCGAAAATGGAGCAGAATTTCCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

981

Amino Acids

107.8

Weight (kDa)

5.69

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 68 7.5e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 163 - 296 1.8e-15 Leucine-rich repeat region
LRR_14 PF23598 406 - 550 3.9e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 668 - 949 1.2e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 670 - 950 2.1e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1354
AccB7I CCANNNNNTGG 1 cut(s) 1571
AccI GTMKAC 1 cut(s) 2043
AccII CGCG 1 cut(s) 1698
AccIII TCCGGA 2 cut(s) 913, 1607
AciI CCGC 6 cut(s) 1696, 1783, 1816, 2109, 2712, 2805
AclI AACGTT 1 cut(s) 2778
AclWI GGATC 3 cut(s) 1248, 1582, 2310
AcoI YGGCCR 3 cut(s) 1552, 1693, 2190
AcuI CTGAAG 3 cut(s) 1260, 1380, 1970
AcvI CACGTG 1 cut(s) 177
AfaI GTAC 8 cut(s) 358, 408, 1019, 1195, 1857, 1958, 2052, 2279
AfiI CCNNNNNNNGG 5 cut(s) 416, 864, 1405, 1571, 2923
AflIII ACRYGT 1 cut(s) 1168
AjnI CCWGG 2 cut(s) 1060, 1750
AjuI GAANNNNNNNTTGG 2 cut(s) 2001, 2033
AloI GAACNNNNNNTCC 2 cut(s) 1845, 1877
Alw21I GWGCWC 3 cut(s) 96, 852, 2204
Alw26I GTCTC 3 cut(s) 223, 338, 2648
AlwI GGATC 3 cut(s) 1248, 1582, 2310
Aor13HI TCCGGA 2 cut(s) 913, 1607
AoxI GGCC 7 cut(s) 976, 1048, 1552, 1693, 1784, 2190, 2645
ArsI GACNNNNNNTTYG 4 cut(s) 1136, 1168, 1286, 1318
Asp700I GAANNNNTTC 2 cut(s) 392, 1112
AspS9I GGNCC 3 cut(s) 977, 1049, 1265
AsuC2I CCSGG 1 cut(s) 773
AvaII GGWCC 1 cut(s) 1265
AxyI CCTNAGG 1 cut(s) 800
BalI TGGCCA 2 cut(s) 1554, 2192
BanII GRGCYC 4 cut(s) 96, 852, 1808, 2204
BbrPI CACGTG 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 1830
Bbv12I GWGCWC 3 cut(s) 96, 852, 2204
BccI CCATC 7 cut(s) 1724, 1736, 2057, 2326, 2447, 2617, 2897
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 261, 295
BciT130I CCWGG 2 cut(s) 1062, 1752
BclI TGATCA 1 cut(s) 1918
BcnI CCSGG 1 cut(s) 773
BcoDI GTCTC 3 cut(s) 223, 338, 2648
BfaI CTAG 7 cut(s) 293, 456, 1812, 1991, 2450, 2469, 2808
BfmI CTRYAG 2 cut(s) 1103, 2236
BfuAI ACCTGC 1 cut(s) 1354
BglI GCCNNNNNGGC 1 cut(s) 2189
BmcAI AGTACT 2 cut(s) 1857, 2279
Bme1390I CCNGG 3 cut(s) 773, 1062, 1752
Bme18I GGWCC 1 cut(s) 1265
BmgT120I GGNCC 3 cut(s) 977, 1049, 1265
BmiI GGNNCC 2 cut(s) 23, 2814
BmrFI CCNGG 3 cut(s) 773, 1062, 1752
BmrI ACTGGG 2 cut(s) 198, 771
BmsI GCATC 6 cut(s) 532, 621, 2170, 2743, 2758, 2901
BmuI ACTGGG 2 cut(s) 198, 771
BoxI GACNNNNGTC 1 cut(s) 2525
BpiI GAAGAC 1 cut(s) 1830
BpmI CTGGAG 3 cut(s) 512, 686, 1872
Bpu10I CCTNAGC 2 cut(s) 728, 1901
BpuEI CTTGAG 7 cut(s) 677, 704, 758, 851, 890, 2725, 2747
BpuMI CCSGG 1 cut(s) 773
Bsa29I ATCGAT 1 cut(s) 1327
BsaAI YACGTR 2 cut(s) 177, 1169
BsaBI GATNNNNATC 1 cut(s) 1746
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 7 cut(s) 479, 523, 1060, 1823, 2184, 2395, 2898
BsaWI WCCGGW 5 cut(s) 28, 913, 1489, 1607, 2815
BsaXI ACNNNNNCTCC 4 cut(s) 1845, 1875, 1897, 1927
Bsc4I CCNNNNNNNGG 5 cut(s) 416, 864, 1405, 1571, 2923
Bse118I RCCGGY 1 cut(s) 2647
Bse1I ACTGG 8 cut(s) 193, 219, 495, 593, 766, 979, 1684, 2436
Bse21I CCTNAGG 1 cut(s) 800
Bse3DI GCAATG 4 cut(s) 564, 1577, 2529, 2787
Bse8I GATNNNNATC 1 cut(s) 1746
BseAI TCCGGA 2 cut(s) 913, 1607
BseBI CCWGG 2 cut(s) 1062, 1752
BseCI ATCGAT 1 cut(s) 1327
BseDI CCNNGG 7 cut(s) 479, 523, 1060, 1823, 2184, 2395, 2898
BseGI GGATG 4 cut(s) 9, 1646, 1793, 2908
BseJI GATNNNNATC 1 cut(s) 1746
BseLI CCNNNNNNNGG 5 cut(s) 416, 864, 1405, 1571, 2923
BseMI GCAATG 4 cut(s) 564, 1577, 2529, 2787
BseNI ACTGG 8 cut(s) 193, 219, 495, 593, 766, 979, 1684, 2436
BseRI GAGGAG 4 cut(s) 35, 44, 932, 1921
BseYI CCCAGC 3 cut(s) 1417, 2534, 2688
BsgI GTGCAG 6 cut(s) 308, 899, 1780, 2294, 2522, 2785
Bsh1236I CGCG 1 cut(s) 1698
BshFI GGCC 7 cut(s) 978, 1050, 1554, 1695, 1786, 2192, 2647
BshVI ATCGAT 1 cut(s) 1327
BsiHKAI GWGCWC 3 cut(s) 96, 852, 2204
BsiSI CCGG 8 cut(s) 13, 29, 773, 914, 1490, 1608, 2648, 2816
BslFI GGGAC 2 cut(s) 1184, 2551
BslI CCNNNNNNNGG 5 cut(s) 416, 864, 1405, 1571, 2923
BsmAI GTCTC 3 cut(s) 223, 338, 2648
BsmFI GGGAC 2 cut(s) 1184, 2551
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 7 cut(s) 978, 1050, 1554, 1695, 1786, 2192, 2647
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 4 cut(s) 96, 852, 1808, 2204
Bsp13I TCCGGA 2 cut(s) 913, 1607
Bsp143I GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
Bsp19I CCATGG 2 cut(s) 479, 2395
BspACI CCGC 6 cut(s) 1696, 1783, 1816, 2109, 2712, 2805
BspANI GGCC 7 cut(s) 978, 1050, 1554, 1695, 1786, 2192, 2647
BspDI ATCGAT 1 cut(s) 1327
BspEI TCCGGA 2 cut(s) 913, 1607
BspFNI CGCG 1 cut(s) 1698
BspLI GGNNCC 2 cut(s) 23, 2814
BspMAI CTGCAG 2 cut(s) 1107, 2240
BspMI ACCTGC 1 cut(s) 1354
BspPI GGATC 3 cut(s) 1248, 1582, 2310
BspTNI GGTCTC 1 cut(s) 223
BsrDI GCAATG 4 cut(s) 564, 1577, 2529, 2787
BsrFI RCCGGY 1 cut(s) 2647
BsrI ACTGG 8 cut(s) 193, 219, 495, 593, 766, 979, 1684, 2436
BssAI RCCGGY 1 cut(s) 2647
BssECI CCNNGG 7 cut(s) 479, 523, 1060, 1823, 2184, 2395, 2898
BssMI GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
BssNAI GTATAC 1 cut(s) 2044
BssT1I CCWWGG 4 cut(s) 479, 523, 2395, 2898
Bst1107I GTATAC 1 cut(s) 2044
Bst2UI CCWGG 2 cut(s) 1062, 1752
Bst4CI ACNGT 1 cut(s) 1198
Bst6I CTCTTC 5 cut(s) 62, 264, 987, 1925, 2585
BstBAI YACGTR 2 cut(s) 177, 1169
BstC8I GCNNGC 6 cut(s) 253, 281, 1220, 1700, 2490, 2505
BstDSI CCRYGG 3 cut(s) 479, 1823, 2395
BstF5I GGATG 4 cut(s) 9, 1646, 1793, 2908
BstFNI CGCG 1 cut(s) 1698
BstKTI GATC 6 cut(s) 841, 1243, 1577, 1672, 1921, 2305
BstMAI GTCTC 3 cut(s) 223, 338, 2648
BstMBI GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
BstMWI GCNNNNNNNGC 7 cut(s) 589, 1812, 1873, 1897, 2189, 2366, 2694
BstNI CCWGG 2 cut(s) 1062, 1752
BstNSI RCATGY 2 cut(s) 1069, 2216
BstPAI GACNNNNGTC 1 cut(s) 2525
BstSCI CCNGG 3 cut(s) 771, 1060, 1750
BstSFI CTRYAG 2 cut(s) 1103, 2236
BstUI CGCG 1 cut(s) 1698
BstV2I GAAGAC 1 cut(s) 1830
BstX2I RGATCY 1 cut(s) 1240
BstXI CCANNNNNNTGG 2 cut(s) 689, 1691
BstYI RGATCY 1 cut(s) 1240
BstZ17I GTATAC 1 cut(s) 2044
Bsu15I ATCGAT 1 cut(s) 1327
Bsu36I CCTNAGG 1 cut(s) 800
BsuRI GGCC 7 cut(s) 978, 1050, 1554, 1695, 1786, 2192, 2647
BsuTUI ATCGAT 1 cut(s) 1327
BtgI CCRYGG 3 cut(s) 479, 1823, 2395
BtgZI GCGATG 1 cut(s) 1702
BtsCI GGATG 4 cut(s) 9, 1646, 1793, 2908
BtsI GCAGTG 1 cut(s) 2714
BtsIMutI CAGTG 6 cut(s) 186, 336, 488, 972, 2195, 2714
BveI ACCTGC 1 cut(s) 1354
Cac8I GCNNGC 6 cut(s) 253, 281, 1220, 1700, 2490, 2505
Cfr10I RCCGGY 1 cut(s) 2647
Cfr13I GGNCC 3 cut(s) 977, 1049, 1265
ClaI ATCGAT 1 cut(s) 1327
CseI GACGC 1 cut(s) 278
Csp6I GTAC 8 cut(s) 357, 407, 1018, 1194, 1856, 1957, 2051, 2278
CviQI GTAC 8 cut(s) 357, 407, 1018, 1194, 1856, 1957, 2051, 2278
DpnI GATC 6 cut(s) 840, 1242, 1576, 1671, 1920, 2304
DpnII GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
EaeI YGGCCR 3 cut(s) 1552, 1693, 2190
Eam1104I CTCTTC 5 cut(s) 62, 264, 987, 1925, 2585
EarI CTCTTC 5 cut(s) 62, 264, 987, 1925, 2585
Ecl136II GAGCTC 3 cut(s) 94, 850, 2202
Eco130I CCWWGG 4 cut(s) 479, 523, 2395, 2898
Eco24I GRGCYC 4 cut(s) 96, 852, 1808, 2204
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 1265
Eco53kI GAGCTC 3 cut(s) 94, 850, 2202
Eco57I CTGAAG 3 cut(s) 1260, 1380, 1970
Eco72I CACGTG 1 cut(s) 177
Eco81I CCTNAGG 1 cut(s) 800
EcoICRI GAGCTC 3 cut(s) 94, 850, 2202
EcoO109I RGGNCCY 2 cut(s) 1049, 1265
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 1060, 1750
EcoT14I CCWWGG 4 cut(s) 479, 523, 2395, 2898
EcoT22I ATGCAT 1 cut(s) 2569
EcoT38I GRGCYC 4 cut(s) 96, 852, 1808, 2204
ErhI CCWWGG 4 cut(s) 479, 523, 2395, 2898
FalI AAGNNNNNCTT 2 cut(s) 2122, 2154
FaqI GGGAC 2 cut(s) 1184, 2551
FbaI TGATCA 1 cut(s) 1918
FblI GTMKAC 1 cut(s) 2043
FokI GGATG 3 cut(s) 1633, 1780, 2915
FriOI GRGCYC 4 cut(s) 96, 852, 1808, 2204
FspBI CTAG 7 cut(s) 293, 456, 1812, 1991, 2450, 2469, 2808
GsaI CCCAGC 3 cut(s) 1421, 2538, 2692
GsuI CTGGAG 3 cut(s) 512, 686, 1872
HaeIII GGCC 7 cut(s) 978, 1050, 1554, 1695, 1786, 2192, 2647
HapII CCGG 8 cut(s) 13, 29, 773, 914, 1490, 1608, 2648, 2816
HgaI GACGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 2876
HindII GTYRAC 1 cut(s) 2876
HindIII AAGCTT 5 cut(s) 299, 581, 827, 992, 1735
HpaII CCGG 8 cut(s) 13, 29, 773, 914, 1490, 1608, 2648, 2816
Hpy166II GTNNAC 5 cut(s) 357, 2044, 2272, 2737, 2876
Hpy8I GTNNAC 5 cut(s) 357, 2044, 2272, 2737, 2876
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 3 cut(s) 1256, 1673, 1981
HpyCH4III ACNGT 1 cut(s) 1198
HpyCH4IV ACGT 3 cut(s) 176, 1168, 2778
HpyF10VI GCNNNNNNNGC 7 cut(s) 589, 1812, 1873, 1897, 2189, 2366, 2694
HpySE526I ACGT 3 cut(s) 176, 1168, 2778
Kpn2I TCCGGA 2 cut(s) 913, 1607
Ksp22I TGATCA 1 cut(s) 1918
Kzo9I GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
LmnI GCTCC 7 cut(s) 27, 667, 1215, 1908, 2292, 2560, 2926
LweI GCATC 6 cut(s) 532, 621, 2170, 2743, 2758, 2901
MaeI CTAG 7 cut(s) 293, 456, 1812, 1991, 2450, 2469, 2808
MaeII ACGT 3 cut(s) 176, 1168, 2778
MaeIII GTNAC 5 cut(s) 1164, 1375, 1707, 2402, 2632
MalI GATC 6 cut(s) 840, 1242, 1576, 1671, 1920, 2304
MboI GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
MfeI CAATTG 3 cut(s) 431, 683, 1425
MflI RGATCY 1 cut(s) 1240
MhlI GDGCHC 4 cut(s) 96, 852, 1808, 2204
MlsI TGGCCA 2 cut(s) 1554, 2192
MluNI TGGCCA 2 cut(s) 1554, 2192
MmeI TCCRAC 1 cut(s) 1186
Mox20I TGGCCA 2 cut(s) 1554, 2192
Mph1103I ATGCAT 1 cut(s) 2569
MroI TCCGGA 2 cut(s) 913, 1607
MroXI GAANNNNTTC 2 cut(s) 392, 1112
MscI TGGCCA 2 cut(s) 1554, 2192
MseI TTAA 2 cut(s) 1589, 1728
MslI CAYNNNNRTG 2 cut(s) 2217, 2424
Msp20I TGGCCA 2 cut(s) 1554, 2192
MspA1I CMGCKG 1 cut(s) 2308
MspI CCGG 8 cut(s) 13, 29, 773, 914, 1490, 1608, 2648, 2816
MspR9I CCNGG 3 cut(s) 773, 1062, 1752
MunI CAATTG 3 cut(s) 431, 683, 1425
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 2 cut(s) 1062, 1752
MvnI CGCG 1 cut(s) 1698
MwoI GCNNNNNNNGC 7 cut(s) 589, 1812, 1873, 1897, 2189, 2366, 2694
NciI CCSGG 1 cut(s) 773
NcoI CCATGG 2 cut(s) 479, 2395
NdeII GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
NlaIV GGNNCC 2 cut(s) 23, 2814
NmeAIII GCCGAG 2 cut(s) 2209, 2523
NmuCI GTSAC 3 cut(s) 1707, 2402, 2632
NsiI ATGCAT 1 cut(s) 2569
NspI RCATGY 2 cut(s) 1069, 2216
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 2 cut(s) 392, 1112
PfeI GAWTC 7 cut(s) 496, 537, 661, 892, 1252, 1381, 1994
PflMI CCANNNNNTGG 1 cut(s) 1571
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
Ppu21I YACGTR 2 cut(s) 177, 1169
PpuMI RGGWCCY 1 cut(s) 1265
PshAI GACNNNNGTC 1 cut(s) 2525
Psp124BI GAGCTC 3 cut(s) 96, 852, 2204
Psp1406I AACGTT 1 cut(s) 2778
Psp5II RGGWCCY 1 cut(s) 1265
Psp6I CCWGG 2 cut(s) 1060, 1750
PspCI CACGTG 1 cut(s) 177
PspFI CCCAGC 3 cut(s) 1417, 2534, 2688
PspGI CCWGG 2 cut(s) 1060, 1750
PspN4I GGNNCC 2 cut(s) 23, 2814
PspPI GGNCC 3 cut(s) 977, 1049, 1265
PspPPI RGGWCCY 1 cut(s) 1265
PstI CTGCAG 2 cut(s) 1107, 2240
PsuI RGATCY 1 cut(s) 1240
PvuII CAGCTG 1 cut(s) 2308
RsaI GTAC 8 cut(s) 358, 408, 1019, 1195, 1857, 1958, 2052, 2279
RsaNI GTAC 8 cut(s) 357, 407, 1018, 1194, 1856, 1957, 2051, 2278
RseI CAYNNNNRTG 2 cut(s) 2217, 2424
SacI GAGCTC 3 cut(s) 96, 852, 2204
SaqAI TTAA 2 cut(s) 1589, 1728
Sau3AI GATC 6 cut(s) 838, 1240, 1574, 1669, 1918, 2302
Sau96I GGNCC 3 cut(s) 977, 1049, 1265
ScaI AGTACT 2 cut(s) 1857, 2279
ScrFI CCNGG 3 cut(s) 773, 1062, 1752
SduI GDGCHC 4 cut(s) 96, 852, 1808, 2204
SfaNI GCATC 6 cut(s) 532, 621, 2170, 2743, 2758, 2901
SfcI CTRYAG 2 cut(s) 1103, 2236
SinI GGWCC 1 cut(s) 1265
SmiMI CAYNNNNRTG 2 cut(s) 2217, 2424
SmlI CTYRAG 7 cut(s) 656, 719, 737, 830, 869, 2726, 2740
SmoI CTYRAG 7 cut(s) 656, 719, 737, 830, 869, 2726, 2740
SsiI CCGC 6 cut(s) 1696, 1783, 1816, 2109, 2712, 2805
SspI AATATT 1 cut(s) 2443
SspMI CTAG 7 cut(s) 293, 456, 1812, 1991, 2450, 2469, 2808
SstI GAGCTC 3 cut(s) 96, 852, 2204
StyD4I CCNGG 3 cut(s) 771, 1060, 1750
StyI CCWWGG 4 cut(s) 479, 523, 2395, 2898
TaaI ACNGT 1 cut(s) 1198
TaiI ACGT 3 cut(s) 179, 1171, 2781
TaqI TCGA 6 cut(s) 96, 267, 540, 1327, 2128, 2831
TaqII GACCGA 1 cut(s) 518
TatI WGTACW 5 cut(s) 356, 1017, 1855, 2050, 2277
TauI GCSGC 3 cut(s) 1698, 1786, 1818
TfiI GAWTC 7 cut(s) 496, 537, 661, 892, 1252, 1381, 1994
Tru1I TTAA 2 cut(s) 1589, 1728
Tru9I TTAA 2 cut(s) 1589, 1728
TscAI CASTG 6 cut(s) 193, 343, 495, 979, 2202, 2714
TseFI GTSAC 3 cut(s) 1707, 2402, 2632
Tsp45I GTSAC 3 cut(s) 1707, 2402, 2632
TspDTI ATGAA 9 cut(s) 1265, 1409, 1445, 1746, 1782, 1787, 1929, 1998, 2330
TspGWI ACGGA 2 cut(s) 342, 1840
TspRI CASTG 6 cut(s) 193, 343, 495, 979, 2202, 2714
Van91I CCANNNNNTGG 1 cut(s) 1571
VpaK11BI GGWCC 1 cut(s) 1265
XceI RCATGY 2 cut(s) 1069, 2216
XmiI GTMKAC 1 cut(s) 2043
XmnI GAANNNNTTC 2 cut(s) 392, 1112
XspI CTAG 7 cut(s) 293, 456, 1812, 1991, 2450, 2469, 2808
ZrmI AGTACT 2 cut(s) 1857, 2279
Zsp2I ATGCAT 1 cut(s) 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.