Rw6G002960

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011518
Physical Location & Seq
Forward (+)
4800603 .. 4803670
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002960.1

Sequence Viewer

Length: 2820 bp
ATGTCGTTCCTCCGGCAACGGCTTCCTCCGGTTCTTCTTCTTCTCTCCCTACTCTCTCTCTCCACATCAACCGAGCTCGACCAACTCTTGAAGCTCAAAACCTCACTTGAAAGTTCAAACCCAACTCTGTTCAGTTCTTGGAAGCAAGAAACTCCCACGTGCAACTTCACTGGGATTGTCTGCAACTCAAATGGGCTGGTCTCAGAAATCAATCTTGCTCAGAAAAACCTTTCTGGCTCTCTTCCTTTCGATGCAATATGCTCGCTCCCACAACTAGAAAAGCTTTCTTTTGGCTCAAATTTCCTGCACGGAAGTCTCACTGAGGATTTGAAGAAGTGTACTAATTTGCAGGAACTGGATTTGGCTAAGAATTCTTTCACAGGGAAAGTACCTGACTTGGGTTCTTTGAGTCAATTGAGGCTGTTGAGTCTGAATGGTAGTGGGGTATCCGGACTTTTTCCATGGAAATCACTGGAGAATCTGACAGAGTTAGCTTTTCTGAGCCTTGGTGATAATAGATTCGATGAAAGTCCGATTACAATGGAGTTGGTAAAGCTTGACAAGCTTTATTGGCTTTACCTCTCAAATTGTAGCATCACAGGTGAAATCCCAGAGGGTATAGGAAACCTGACTCTGCTTGAGAATCTGGAGCTTTCGGATAACCACTTGGTTGGTGAAATTCCTCAGAGTATTACAAGTCTCAAGAAGCTCAGGCAACTTGAGCTTTATGCTAATTCCCTGACTGGGAAAATCCCAGTTGGGGTTGGCAACCTCACAAGCCTTAGGAATTTTGATGTTTCAAATAACATGCTTGAGGGTGACCTCTCTGAGCTCAAATCCTTGACAGGGATTGAGTCTCTGCAGCTTTATGAGAATCAATTAGTTGGCGAAGTTCCAGAGGAGTTTGGAGAGTTCAAGAACCTTTATCAGCTCTCACTTTACAAGAACATGCTCACTGGCCCTCTTCCTCAGAAGCTTGGTTCTTGGTCTGGCCTGGAGTACATTGATGTTTCAGAGAACTACTTGACTGGCCCTATACCCCCTGGCATGTGCAAAAATGAGAAACTGATTGATTTTCTTCTGCTGCAGAACAATTTCACAGGTGGGATTCCTGAGTCCTATGCAAATTGCAAGTCTTTAGTTCGGTTACGTGTGAATAACAATTCACTTTCGGGTACTGTCCCTGTTGGAATTTGGAGCTTGCCACGTGTTGTAGTTATGGATCTTTCTATGAATCAGTTTGAAGGTCCTCTGACTTCAGATGTTGGTAAGGCAAATTCTCTATCATTATTACTCTTAGCTAATAATCGATTTTCGGGTGAATTGCCTCTTATGAACCAATTTGAGGGTCAAATCCCAGCAACAATTGGGAACTTGAAGAAATTAGGCAGTTTTCATTTAGAACAAAATTTGTTGTCTGGTGCTATACCGGAATCAATAGGCTCATGTGCTAGGATTTCTGAAATCAACCTTGCCCATAACAATCTTTCTGGCCAAATCCCTCCAAGTCTTGGATCATTGCCTAACTTAAACTCCTTGAACTTATCTGGAAACCAACTTTCAAGTGAAGTTCCTGCTACTTTGTCATCCCTCAAACTCAGCCTTCTTGATCTTACAAACAACCAGTTGATTGGTCGCGTGCCCGAGTTACTATCCATTGCAGCTTTTAATGAAAGCTTTGATGGAAATCCTGGTTTGTGCAGCCAGACCTTGAAGAATTTGCGGCCTTTACTTGCTCTGCTTATTTTGCTTGGTGTGTTCTTGCTTTGGAAGCTAAGGAGGAGCAGTCTTGATCAACCATTGAAGAGTAATTCATGGACTATGAAGCATTACCATGTGCTTACCTTCACAGAGAATGAAATCCTAGATTCAATCAAAGCTGAAAATTTGATTGGCAAAGGAGGGTCAGGAAATGTATACAAAGTACAACTGAGAGATGGGAAAGCACTTGCTGTGAAGCACATTTGGACTTCACCAGACTCCGCTCACCAGAAAACTTATCGAAGCACCACTTCCATTTTGAAGAAGTCTAAATCCAGCTCATCGGAATATGATGCCGAGGTGGCCACACTGAGCTCATTGAGGCATGTGAATGTGGTGAAGCTATACTGCAGCATTAGTAGTGAGGACAGCAATCTGCTGGTTTACGAGTACTTTCCCAATGGGAGCTTATGGGATCAGCTGCACATGAATAACAAGGTCAAGATGGGGTGGGAAGTGAGGTATGAGATTGCTTTGGGGGCTGCAAGAGGATTGGAGTATCTTCACCATGGTAGTCACAGACCTGTTATACACAGAGATGTGAAGTCCAGTAATATTCTGCTAGATGGAGATTGGAAGCCTAGAATTGCTGATTTTGGGCTTGCCAAGATTGTGCAGGCTGGTGGTGAAATGACTCATGTCATTGCTGGGACACTCGGCTACATAGCTCCTGAGTATGCATATACCTACAAGGTAAATGAGAAGAGTGATGTGTATAGCTTTGGGGTGGTCTTGATGGAGTTGGTGACAGGAAAGAGGCCGGTGGAGACTGATTTTGGAGAGAACAAGGACATAGTGAGCTGGGTTTGCAGCAAAATGCAGTGCAGAGAGAGTGAGCTTGAGTTGGTGGACTCAAGCATTGACGATGCTCTACAAGAAGATGCCCTCAACGTTTTGAGCATTGCAATTCGCTGCACCGCTAGGGTTCCGGTTCTAAGACCCTCGATGAGAATGGTGGTTCAAATGCTGGAAGAAGCACAACCTTGTCAACTCACCAGCATAAGCATCACCAAGGATGGTGAAAATAACCCGAAAATGGAGCAGAATTTCCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009960 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

939

Amino Acids

103.58

Weight (kDa)

5.57

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 62 3.6e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 157 - 293 2e-14 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 626 - 908 3.6e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 628 - 908 6.7e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1511
AccBSI CCGCTC 1 cut(s) 1985
AccI GTMKAC 1 cut(s) 1917
AccII CGCG 1 cut(s) 1638
AccIII TCCGGA 1 cut(s) 449
AciI CCGC 3 cut(s) 1723, 1983, 2679
AclI AACGTT 1 cut(s) 2652
AclWI GGATC 3 cut(s) 1230, 1522, 2184
AcoI YGGCCR 2 cut(s) 1492, 2064
AcuI CTGAAG 1 cut(s) 1242
AcvI CACGTG 2 cut(s) 159, 1208
AfaI GTAC 6 cut(s) 340, 390, 1001, 1177, 1926, 2153
AfiI CCNNNNNNNGG 7 cut(s) 398, 744, 760, 846, 1345, 1511, 2797
AflIII ACRYGT 2 cut(s) 1150, 1207
AjnI CCWGG 3 cut(s) 993, 1042, 1690
AjuI GAANNNNNNNTTGG 2 cut(s) 1875, 1907
Alw21I GWGCWC 3 cut(s) 78, 834, 2078
Alw26I GTCTC 5 cut(s) 205, 320, 704, 861, 2522
AlwI GGATC 3 cut(s) 1230, 1522, 2184
AlwNI CAGNNNCTG 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 1643
Aor13HI TCCGGA 1 cut(s) 449
AoxI GGCC 7 cut(s) 958, 991, 1030, 1492, 1724, 2064, 2519
ApeKI GCWGC 9 cut(s) 862, 1084, 1661, 1701, 2112, 2182, 2243, 2571, 2673
ArsI GACNNNNNNTTYG 2 cut(s) 1118, 1150
Asp700I GAANNNNTTC 2 cut(s) 374, 1094
AspS9I GGNCC 3 cut(s) 959, 1031, 1247
AvaI CYCGRG 1 cut(s) 1643
AvaII GGWCC 1 cut(s) 1247
AxyI CCTNAGG 1 cut(s) 782
BaeGI GKGCMC 1 cut(s) 1644
BalI TGGCCA 2 cut(s) 1494, 2066
BanII GRGCYC 3 cut(s) 78, 834, 2078
BbrPI CACGTG 2 cut(s) 159, 1208
Bbv12I GWGCWC 3 cut(s) 78, 834, 2078
BbvI GCAGC 9 cut(s) 874, 1071, 1673, 1713, 2124, 2169, 2230, 2583, 2660
BccI CCATC 6 cut(s) 1676, 1931, 2200, 2321, 2491, 2771
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 3 cut(s) 995, 1044, 1692
BciVI GTATCC 1 cut(s) 457
BclI TGATCA 1 cut(s) 1792
BcoDI GTCTC 5 cut(s) 205, 320, 704, 861, 2522
BfaI CTAG 6 cut(s) 275, 1452, 1865, 2324, 2343, 2682
BfmI CTRYAG 3 cut(s) 860, 1085, 2110
BfuI GTATCC 1 cut(s) 457
BglI GCCNNNNNGGC 1 cut(s) 2063
BmcAI AGTACT 1 cut(s) 2153
Bme1390I CCNGG 3 cut(s) 995, 1044, 1692
Bme18I GGWCC 1 cut(s) 1247
BmeT110I CYCGRG 1 cut(s) 1643
BmgT120I GGNCC 3 cut(s) 959, 1031, 1247
BmiI GGNNCC 1 cut(s) 2688
BmrFI CCNGG 3 cut(s) 995, 1044, 1692
BmrI ACTGGG 3 cut(s) 180, 749, 753
BmsI GCATC 6 cut(s) 241, 603, 2044, 2617, 2632, 2775
BmuI ACTGGG 3 cut(s) 180, 749, 753
BoxI GACNNNNGTC 1 cut(s) 2399
BpmI CTGGAG 3 cut(s) 494, 668, 1016
Bpu10I CCTNAGC 2 cut(s) 710, 1775
BpuEI CTTGAG 6 cut(s) 659, 686, 740, 833, 2599, 2621
Bsa29I ATCGAT 1 cut(s) 1309
BsaAI YACGTR 3 cut(s) 159, 1151, 1208
BsaBI GATNNNNATC 1 cut(s) 1686
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 6 cut(s) 461, 505, 1042, 2058, 2269, 2772
BsaWI WCCGGW 4 cut(s) 28, 449, 1429, 2689
BsaXI ACNNNNNCTCC 4 cut(s) 1517, 1547, 1771, 1801
Bsc4I CCNNNNNNNGG 7 cut(s) 398, 744, 760, 846, 1345, 1511, 2797
Bse118I RCCGGY 1 cut(s) 2521
Bse1I ACTGG 9 cut(s) 175, 360, 477, 748, 755, 961, 1033, 1624, 2310
Bse21I CCTNAGG 1 cut(s) 782
Bse3DI GCAATG 4 cut(s) 1517, 1656, 2403, 2661
Bse8I GATNNNNATC 1 cut(s) 1686
BseAI TCCGGA 1 cut(s) 449
BseBI CCWGG 3 cut(s) 995, 1044, 1692
BseCI ATCGAT 1 cut(s) 1309
BseDI CCNNGG 6 cut(s) 461, 505, 1042, 2058, 2269, 2772
BseGI GGATG 2 cut(s) 1586, 2782
BseJI GATNNNNATC 1 cut(s) 1686
BseLI CCNNNNNNNGG 7 cut(s) 398, 744, 760, 846, 1345, 1511, 2797
BseMI GCAATG 4 cut(s) 1517, 1656, 2403, 2661
BseNI ACTGG 9 cut(s) 175, 360, 477, 748, 755, 961, 1033, 1624, 2310
BseRI GAGGAG 2 cut(s) 914, 1795
BseSI GKGCMC 1 cut(s) 1644
BseXI GCAGC 9 cut(s) 874, 1071, 1673, 1713, 2124, 2169, 2230, 2583, 2660
BseYI CCCAGC 3 cut(s) 1357, 2408, 2562
BsgI GTGCAG 6 cut(s) 290, 1720, 2168, 2396, 2605, 2659
Bsh1236I CGCG 1 cut(s) 1638
BshFI GGCC 7 cut(s) 960, 993, 1032, 1494, 1726, 2066, 2521
BshVI ATCGAT 1 cut(s) 1309
BsiHKAI GWGCWC 3 cut(s) 78, 834, 2078
BsiHKCI CYCGRG 1 cut(s) 1643
BsiSI CCGG 6 cut(s) 13, 29, 450, 1430, 2522, 2690
BslFI GGGAC 2 cut(s) 1166, 2425
BslI CCNNNNNNNGG 7 cut(s) 398, 744, 760, 846, 1345, 1511, 2797
BsmAI GTCTC 5 cut(s) 205, 320, 704, 861, 2522
BsmFI GGGAC 2 cut(s) 1166, 2425
BsnI GGCC 7 cut(s) 960, 993, 1032, 1494, 1726, 2066, 2521
Bso31I GGTCTC 1 cut(s) 205
BsoBI CYCGRG 1 cut(s) 1643
Bsp1286I GDGCHC 4 cut(s) 78, 834, 1644, 2078
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
Bsp19I CCATGG 2 cut(s) 461, 2269
BspACI CCGC 3 cut(s) 1723, 1983, 2679
BspANI GGCC 7 cut(s) 960, 993, 1032, 1494, 1726, 2066, 2521
BspDI ATCGAT 1 cut(s) 1309
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 1 cut(s) 1638
BspLI GGNNCC 1 cut(s) 2688
BspMAI CTGCAG 3 cut(s) 864, 1089, 2114
BspPI GGATC 3 cut(s) 1230, 1522, 2184
BspTNI GGTCTC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 1985
BsrDI GCAATG 4 cut(s) 1517, 1656, 2403, 2661
BsrFI RCCGGY 1 cut(s) 2521
BsrI ACTGG 9 cut(s) 175, 360, 477, 748, 755, 961, 1033, 1624, 2310
BssAI RCCGGY 1 cut(s) 2521
BssECI CCNNGG 6 cut(s) 461, 505, 1042, 2058, 2269, 2772
BssMI GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
BssNAI GTATAC 1 cut(s) 1918
BssT1I CCWWGG 4 cut(s) 461, 505, 2269, 2772
Bst1107I GTATAC 1 cut(s) 1918
Bst2UI CCWGG 3 cut(s) 995, 1044, 1692
Bst4CI ACNGT 1 cut(s) 1180
Bst6I CTCTTC 4 cut(s) 246, 969, 1799, 2459
BstBAI YACGTR 3 cut(s) 159, 1151, 1208
BstC8I GCNNGC 5 cut(s) 263, 1202, 1640, 2364, 2379
BstDSI CCRYGG 2 cut(s) 461, 2269
BstEII GGTNACC 1 cut(s) 818
BstF5I GGATG 2 cut(s) 1586, 2782
BstFNI CGCG 1 cut(s) 1638
BstKTI GATC 5 cut(s) 1225, 1517, 1612, 1795, 2179
BstMAI GTCTC 5 cut(s) 205, 320, 704, 861, 2522
BstMBI GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
BstMWI GCNNNNNNNGC 8 cut(s) 562, 571, 721, 1747, 1771, 2063, 2240, 2568
BstNI CCWGG 3 cut(s) 995, 1044, 1692
BstNSI RCATGY 4 cut(s) 811, 952, 1051, 2090
BstPAI GACNNNNGTC 1 cut(s) 2399
BstPI GGTNACC 1 cut(s) 818
BstSCI CCNGG 3 cut(s) 993, 1042, 1690
BstSFI CTRYAG 3 cut(s) 860, 1085, 2110
BstSLI GKGCMC 1 cut(s) 1644
BstUI CGCG 1 cut(s) 1638
BstV1I GCAGC 9 cut(s) 874, 1071, 1673, 1713, 2124, 2169, 2230, 2583, 2660
BstX2I RGATCY 1 cut(s) 1222
BstXI CCANNNNNNTGG 2 cut(s) 671, 1631
BstYI RGATCY 1 cut(s) 1222
BstZ17I GTATAC 1 cut(s) 1918
Bsu15I ATCGAT 1 cut(s) 1309
Bsu36I CCTNAGG 1 cut(s) 782
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 7 cut(s) 960, 993, 1032, 1494, 1726, 2066, 2521
BsuTUI ATCGAT 1 cut(s) 1309
BtgI CCRYGG 2 cut(s) 461, 2269
BtsCI GGATG 2 cut(s) 1586, 2782
BtsI GCAGTG 1 cut(s) 2588
BtsIMutI CAGTG 6 cut(s) 168, 318, 470, 954, 2069, 2588
Cac8I GCNNGC 5 cut(s) 263, 1202, 1640, 2364, 2379
CaiI CAGNNNCTG 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 2521
Cfr13I GGNCC 3 cut(s) 959, 1031, 1247
ClaI ATCGAT 1 cut(s) 1309
Csp6I GTAC 6 cut(s) 339, 389, 1000, 1176, 1925, 2152
CviQI GTAC 6 cut(s) 339, 389, 1000, 1176, 1925, 2152
DpnI GATC 5 cut(s) 1224, 1516, 1611, 1794, 2178
DpnII GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
EaeI YGGCCR 2 cut(s) 1492, 2064
Eam1104I CTCTTC 4 cut(s) 246, 969, 1799, 2459
EarI CTCTTC 4 cut(s) 246, 969, 1799, 2459
Ecl136II GAGCTC 3 cut(s) 76, 832, 2076
Eco130I CCWWGG 4 cut(s) 461, 505, 2269, 2772
Eco24I GRGCYC 3 cut(s) 78, 834, 2078
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 1247
Eco53kI GAGCTC 3 cut(s) 76, 832, 2076
Eco57I CTGAAG 1 cut(s) 1242
Eco72I CACGTG 2 cut(s) 159, 1208
Eco81I CCTNAGG 1 cut(s) 782
Eco88I CYCGRG 1 cut(s) 1643
Eco91I GGTNACC 1 cut(s) 818
EcoICRI GAGCTC 3 cut(s) 76, 832, 2076
EcoO109I RGGNCCY 1 cut(s) 1247
EcoO65I GGTNACC 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 993, 1042, 1690
EcoT14I CCWWGG 4 cut(s) 461, 505, 2269, 2772
EcoT22I ATGCAT 1 cut(s) 2443
EcoT38I GRGCYC 3 cut(s) 78, 834, 2078
ErhI CCWWGG 4 cut(s) 461, 505, 2269, 2772
FalI AAGNNNNNCTT 2 cut(s) 1996, 2028
FaqI GGGAC 2 cut(s) 1166, 2425
FbaI TGATCA 1 cut(s) 1792
FblI GTMKAC 1 cut(s) 1917
FokI GGATG 2 cut(s) 1573, 2789
FriOI GRGCYC 3 cut(s) 78, 834, 2078
FspBI CTAG 6 cut(s) 275, 1452, 1865, 2324, 2343, 2682
GsaI CCCAGC 3 cut(s) 1361, 2412, 2566
GsuI CTGGAG 3 cut(s) 494, 668, 1016
HaeIII GGCC 7 cut(s) 960, 993, 1032, 1494, 1726, 2066, 2521
HapII CCGG 6 cut(s) 13, 29, 450, 1430, 2522, 2690
HincII GTYRAC 1 cut(s) 2750
HindII GTYRAC 1 cut(s) 2750
HindIII AAGCTT 5 cut(s) 281, 554, 563, 974, 1675
HpaII CCGG 6 cut(s) 13, 29, 450, 1430, 2522, 2690
Hpy166II GTNNAC 5 cut(s) 339, 1918, 2146, 2611, 2750
Hpy8I GTNNAC 5 cut(s) 339, 1918, 2146, 2611, 2750
HpyAV CCTTC 3 cut(s) 1238, 1613, 1855
HpyCH4III ACNGT 1 cut(s) 1180
HpyCH4IV ACGT 4 cut(s) 158, 1150, 1207, 2652
HpyF10VI GCNNNNNNNGC 8 cut(s) 562, 571, 721, 1747, 1771, 2063, 2240, 2568
HpySE526I ACGT 4 cut(s) 158, 1150, 1207, 2652
Kpn2I TCCGGA 1 cut(s) 449
Ksp22I TGATCA 1 cut(s) 1792
Kzo9I GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
LmnI GCTCC 7 cut(s) 270, 649, 1197, 1782, 2166, 2434, 2800
Lsp1109I GCAGC 9 cut(s) 874, 1071, 1673, 1713, 2124, 2169, 2230, 2583, 2660
LweI GCATC 6 cut(s) 241, 603, 2044, 2617, 2632, 2775
MaeI CTAG 6 cut(s) 275, 1452, 1865, 2324, 2343, 2682
MaeII ACGT 4 cut(s) 158, 1150, 1207, 2652
MaeIII GTNAC 5 cut(s) 818, 1146, 1647, 2276, 2506
MalI GATC 5 cut(s) 1224, 1516, 1611, 1794, 2178
MbiI CCGCTC 1 cut(s) 1985
MboI GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
MfeI CAATTG 2 cut(s) 413, 1365
MflI RGATCY 1 cut(s) 1222
MhlI GDGCHC 4 cut(s) 78, 834, 1644, 2078
MlsI TGGCCA 2 cut(s) 1494, 2066
MluNI TGGCCA 2 cut(s) 1494, 2066
MlyI GAGTC 8 cut(s) 418, 436, 625, 863, 1124, 1973, 2389, 2606
MmeI TCCRAC 1 cut(s) 1168
Mox20I TGGCCA 2 cut(s) 1494, 2066
Mph1103I ATGCAT 1 cut(s) 2443
MroI TCCGGA 1 cut(s) 449
MroXI GAANNNNTTC 2 cut(s) 374, 1094
MscI TGGCCA 2 cut(s) 1494, 2066
MseI TTAA 2 cut(s) 1529, 1668
MslI CAYNNNNRTG 3 cut(s) 1833, 2091, 2298
Msp20I TGGCCA 2 cut(s) 1494, 2066
MspA1I CMGCKG 1 cut(s) 2182
MspI CCGG 6 cut(s) 13, 29, 450, 1430, 2522, 2690
MspR9I CCNGG 3 cut(s) 995, 1044, 1692
MunI CAATTG 2 cut(s) 413, 1365
MvaI CCWGG 3 cut(s) 995, 1044, 1692
MvnI CGCG 1 cut(s) 1638
MwoI GCNNNNNNNGC 8 cut(s) 562, 571, 721, 1747, 1771, 2063, 2240, 2568
NcoI CCATGG 2 cut(s) 461, 2269
NdeII GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
NlaIV GGNNCC 1 cut(s) 2688
NmeAIII GCCGAG 2 cut(s) 2083, 2397
NmuCI GTSAC 3 cut(s) 818, 2276, 2506
NsiI ATGCAT 1 cut(s) 2443
NspI RCATGY 4 cut(s) 811, 952, 1051, 2090
PdmI GAANNNNTTC 2 cut(s) 374, 1094
PfeI GAWTC 8 cut(s) 478, 519, 643, 874, 1108, 1234, 1433, 1868
PflMI CCANNNNNTGG 1 cut(s) 1511
PleI GAGTC 8 cut(s) 417, 435, 625, 862, 1123, 1973, 2389, 2606
PmaCI CACGTG 2 cut(s) 159, 1208
PmlI CACGTG 2 cut(s) 159, 1208
PpsI GAGTC 8 cut(s) 417, 435, 625, 862, 1123, 1973, 2389, 2606
Ppu21I YACGTR 3 cut(s) 159, 1151, 1208
PpuMI RGGWCCY 1 cut(s) 1247
PshAI GACNNNNGTC 1 cut(s) 2399
Psp124BI GAGCTC 3 cut(s) 78, 834, 2078
Psp1406I AACGTT 1 cut(s) 2652
Psp5II RGGWCCY 1 cut(s) 1247
Psp6I CCWGG 3 cut(s) 993, 1042, 1690
PspCI CACGTG 2 cut(s) 159, 1208
PspEI GGTNACC 1 cut(s) 818
PspFI CCCAGC 3 cut(s) 1357, 2408, 2562
PspGI CCWGG 3 cut(s) 993, 1042, 1690
PspN4I GGNNCC 1 cut(s) 2688
PspPI GGNCC 3 cut(s) 959, 1031, 1247
PspPPI RGGWCCY 1 cut(s) 1247
PstI CTGCAG 3 cut(s) 864, 1089, 2114
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 1222
PvuII CAGCTG 1 cut(s) 2182
RsaI GTAC 6 cut(s) 340, 390, 1001, 1177, 1926, 2153
RsaNI GTAC 6 cut(s) 339, 389, 1000, 1176, 1925, 2152
RseI CAYNNNNRTG 3 cut(s) 1833, 2091, 2298
SacI GAGCTC 3 cut(s) 78, 834, 2078
SaqAI TTAA 2 cut(s) 1529, 1668
Sau3AI GATC 5 cut(s) 1222, 1514, 1609, 1792, 2176
Sau96I GGNCC 3 cut(s) 959, 1031, 1247
ScaI AGTACT 1 cut(s) 2153
SchI GAGTC 8 cut(s) 418, 436, 625, 863, 1124, 1973, 2389, 2606
ScrFI CCNGG 3 cut(s) 995, 1044, 1692
SduI GDGCHC 4 cut(s) 78, 834, 1644, 2078
SfaNI GCATC 6 cut(s) 241, 603, 2044, 2617, 2632, 2775
SfcI CTRYAG 3 cut(s) 860, 1085, 2110
SinI GGWCC 1 cut(s) 1247
SmiMI CAYNNNNRTG 3 cut(s) 1833, 2091, 2298
SmlI CTYRAG 6 cut(s) 638, 701, 719, 812, 2600, 2614
SmoI CTYRAG 6 cut(s) 638, 701, 719, 812, 2600, 2614
SsiI CCGC 3 cut(s) 1723, 1983, 2679
SspI AATATT 1 cut(s) 2317
SspMI CTAG 6 cut(s) 275, 1452, 1865, 2324, 2343, 2682
SstI GAGCTC 3 cut(s) 78, 834, 2078
StyD4I CCNGG 3 cut(s) 993, 1042, 1690
StyI CCWWGG 4 cut(s) 461, 505, 2269, 2772
TaaI ACNGT 1 cut(s) 1180
TaiI ACGT 4 cut(s) 161, 1153, 1210, 2655
TaqI TCGA 6 cut(s) 78, 249, 522, 1309, 2002, 2705
TatI WGTACW 4 cut(s) 338, 999, 1924, 2151
TauI GCSGC 1 cut(s) 1726
TfiI GAWTC 8 cut(s) 478, 519, 643, 874, 1108, 1234, 1433, 1868
Tru1I TTAA 2 cut(s) 1529, 1668
Tru9I TTAA 2 cut(s) 1529, 1668
TscAI CASTG 6 cut(s) 175, 325, 477, 961, 2076, 2588
TseFI GTSAC 3 cut(s) 818, 2276, 2506
TseI GCWGC 9 cut(s) 862, 1084, 1661, 1701, 2112, 2182, 2243, 2571, 2673
Tsp45I GTSAC 3 cut(s) 818, 2276, 2506
TspDTI ATGAA 9 cut(s) 540, 1247, 1349, 1385, 1686, 1803, 1838, 1872, 2204
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 6 cut(s) 175, 325, 477, 961, 2076, 2588
Van91I CCANNNNNTGG 1 cut(s) 1511
VpaK11BI GGWCC 1 cut(s) 1247
XceI RCATGY 4 cut(s) 811, 952, 1051, 2090
XmiI GTMKAC 1 cut(s) 1917
XmnI GAANNNNTTC 2 cut(s) 374, 1094
XspI CTAG 6 cut(s) 275, 1452, 1865, 2324, 2343, 2682
ZrmI AGTACT 1 cut(s) 2153
Zsp2I ATGCAT 1 cut(s) 2443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.