FvH4_2g03071

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017235
Physical Location & Seq
Forward (+)
2379994 .. 2381439
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03071.t1

Sequence Viewer

Length: 1446 bp
ATGATTCCAGAGTCCTATTCAGTCGAAAGTACCATTAATAATGAAGCTTGTGAAGTTGAGAATCTTCAAGCTCAAAGTGAGATGACTAATGGTGTTGCAGTGGAGTCTGTCACTGTGGAGTCCTTGCATGCTGATAACTTGACTTCTGCTATGACAGAGCAGGTGCAGGTTAATGAGCATTATGTGTTTGATTGCAGTGATTATGAAACAGAAGGAAGGACTGAGTACGTTGTGTGTGGACTTGGGAGTGTCTCTGACAATACAGCTTCTGAAGAAAAGAACAAGCCGGAGATTGGAGTATCTGACAACATTGGAGAAGTAATGGTTGATTTCAAATATGAAGAAGTTGATCAACAAGATGAAGCACCAGCCGACATTAAGGATCTGGATGAGAAGGGTCAGCTACAGACCGGAGCGTTAGTAGATTTGGAGCATCTTCATGCTCAAACTGAGATTCCTACTGGTGCTGACGTTGAGTTTGTCACTTTGGATGCCTTGCATTATGATAACATGTTTGCTTCGAAGGAGGGGGTCTGTTTTGGTCATGGAACAATGTTTGACTATGATGTATTGGGAAACACAATGGAGACTATCTCCGAAGTTGAATCTTTTGAAGAGAAGGATCAGTCACAGATTGGAGCAGCTGACGACAATGAAGAAATAGAGGTTGATCTAAACTATAAAGTTGATGAAGAGGGTGAAGCAGAAGATGGCATTGAGTCTTTGGAGGAGAAGGATCTGTCAGAAACTGAATTCAAGGTTTCCATGGTGAATGTTGTAGCAATTTCTATATTGATTTCTGCATTGATTTCTGTCTTAATCGGATCCACAGCCTCCAGTTATGTGAAGAAAGGAAACAGCTCAACCTTGAATCTTGTTATCTCCATATGCCAGCAAGCACTGACCAATAAGCAATTCAATGCAAGTCCTGATGTGCCATGTAGCACTATACACATTTTCCAGGACATACCCTCTTCATGGAACTGGATTAAAGAGCCATTGAATTCCAAACTGAGCAACTTGCAGAAGAGTTCATCGTACCGCTTGATGGGACAGGATGCATCAGCTAAACATGAAATCCAGCAAAAGAGAGGCGCTACCGCTGTATGTCAACAACCATTGCTGACTAAGTTGAATACAAGTCTCATCATGCCAGTTGGTACTAAGCACAAGATTCACAAAAGGCCCTCTTCTTGGAATTGGATCACAGAGTCGTGCCATTCTGAAATGAGCAGCTTACAGAACAGTTCATACCAAACACAAGGACTAAATGAATTATATAAATCTGAGAGCCAGGAGAAGAGTGACTCAGGAAAAACCATAGAAGAGAGGCCTTGGCTCCAATGGACTCCTCAATGGGCTCATTGTCTTTATGGGAGGTCTAAAACAATTGACAAAAACACAATCAAGAATTGTTGCTCATCAACAATGCTAATTCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

482

Amino Acids

53.38

Weight (kDa)

4.44

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03071
rosa_chinensis RchiOBHm_Chr6g0247941
rosa_laevigata RLG00000015171
rosa_multiflora Rmu_sc0006106.1_g000013
rosa_roxburghii Rroxscaffold_180G00433170 Rroxscaffold_7G00213570
rosa_rugosa Rorug05G0529600
rosa_samantha Rh6AG044400 Rh6BG039200 Rh6BG039300 Rh6CG038000 Rh6DG035000
rosa_wichuraiana Rw6G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc36I ACCTGC 2 cut(s) 151, 157
AciI CCGC 2 cut(s) 1042, 1101
AclWI GGATC 6 cut(s) 390, 630, 744, 819, 832, 1211
AcsI RAATTY 2 cut(s) 752, 1003
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 4 cut(s) 31, 227, 1040, 1162
AfiI CCNNNNNNNGG 4 cut(s) 293, 978, 1048, 1194
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 9 cut(s) 68, 334, 605, 614, 757, 871, 919, 1003, 1135
AjnI CCWGG 2 cut(s) 960, 1293
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 8 cut(s) 47, 71, 266, 403, 644, 861, 1067, 1236
AluI AGCT 8 cut(s) 47, 71, 266, 403, 644, 861, 1067, 1236
Alw26I GTCTC 3 cut(s) 256, 581, 1148
AlwI GGATC 6 cut(s) 390, 630, 744, 819, 832, 1211
AlwNI CAGNNNCTG 2 cut(s) 269, 749
AoxI GGCC 2 cut(s) 1184, 1331
ApeKI GCWGC 2 cut(s) 641, 1233
ApoI RAATTY 2 cut(s) 752, 1003
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AseI ATTAAT 1 cut(s) 36
AspLEI GCGC 1 cut(s) 1097
AspS9I GGNCC 1 cut(s) 1185
AsuHPI GGTGA 2 cut(s) 710, 781
AsuII TTCGAA 1 cut(s) 521
BamHI GGATCC 1 cut(s) 824
BanII GRGCYC 1 cut(s) 1363
BbvI GCAGC 2 cut(s) 653, 1245
BccI CCATC 2 cut(s) 704, 1042
BciT130I CCWGG 2 cut(s) 962, 1295
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 3 cut(s) 256, 581, 1148
BfmI CTRYAG 1 cut(s) 404
BfoI RGCGCY 1 cut(s) 1098
BfuAI ACCTGC 2 cut(s) 151, 157
BisI GCNGC 2 cut(s) 642, 1234
BlsI GCNGC 2 cut(s) 643, 1235
Bme1390I CCNGG 2 cut(s) 962, 1295
BmgT120I GGNCC 1 cut(s) 1185
BmiI GGNNCC 2 cut(s) 826, 1340
BmrFI CCNGG 2 cut(s) 962, 1295
BmsI GCATC 4 cut(s) 442, 481, 1048, 1070
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 1 cut(s) 820
Bpu14I TTCGAA 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 765, 1334
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 978, 1048, 1194
Bse1I ACTGG 4 cut(s) 466, 837, 989, 1154
Bse3DI GCAATG 1 cut(s) 1118
BseBI CCWGG 2 cut(s) 962, 1295
BseDI CCNNGG 2 cut(s) 765, 1334
BseGI GGATG 3 cut(s) 394, 496, 1063
BseLI CCNNNNNNNGG 4 cut(s) 293, 978, 1048, 1194
BseMI GCAATG 1 cut(s) 1118
BseMII CTCAG 5 cut(s) 213, 441, 1004, 1278, 1323
BseNI ACTGG 4 cut(s) 466, 837, 989, 1154
BseRI GAGGAG 2 cut(s) 743, 1341
BseXI GCAGC 2 cut(s) 653, 1245
BsgI GTGCAG 1 cut(s) 185
BshFI GGCC 2 cut(s) 1186, 1333
BsiSI CCGG 2 cut(s) 287, 411
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 4 cut(s) 293, 978, 1048, 1194
BsmAI GTCTC 3 cut(s) 256, 581, 1148
BsmFI GGGAC 1 cut(s) 1065
BsnI GGCC 2 cut(s) 1186, 1333
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 1363
Bsp143I GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 2 cut(s) 1042, 1101
BspANI GGCC 2 cut(s) 1186, 1333
BspCNI CTCAG 5 cut(s) 214, 442, 1005, 1279, 1322
BspLI GGNNCC 2 cut(s) 826, 1340
BspMI ACCTGC 2 cut(s) 151, 157
BspPI GGATC 6 cut(s) 390, 630, 744, 819, 832, 1211
BspT104I TTCGAA 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 1118
BsrI ACTGG 4 cut(s) 466, 837, 989, 1154
BssECI CCNNGG 2 cut(s) 765, 1334
BssMI GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
BssT1I CCWWGG 2 cut(s) 765, 1334
Bst2UI CCWGG 2 cut(s) 962, 1295
Bst4CI ACNGT 2 cut(s) 115, 1247
Bst6I CTCTTC 7 cut(s) 609, 687, 979, 1022, 1195, 1295, 1320
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 3 cut(s) 129, 893, 897
BstDEI CTNAG 7 cut(s) 222, 450, 1013, 1128, 1164, 1287, 1309
BstDSI CCRYGG 1 cut(s) 765
BstF5I GGATG 3 cut(s) 394, 496, 1063
BstH2I RGCGCY 1 cut(s) 1098
BstHHI GCGC 1 cut(s) 1097
BstKTI GATC 7 cut(s) 352, 385, 625, 673, 739, 827, 1206
BstMAI GTCTC 3 cut(s) 256, 581, 1148
BstMBI GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
BstNI CCWGG 2 cut(s) 962, 1295
BstNSI RCATGY 2 cut(s) 131, 514
BstSCI CCNGG 2 cut(s) 960, 1293
BstSFI CTRYAG 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 653, 1245
BstX2I RGATCY 3 cut(s) 382, 736, 824
BstYI RGATCY 3 cut(s) 382, 736, 824
BsuRI GGCC 2 cut(s) 1186, 1333
BtgI CCRYGG 1 cut(s) 765
BtsCI GGATG 3 cut(s) 394, 496, 1063
BtsI GCAGTG 2 cut(s) 105, 202
BtsIMutI CAGTG 4 cut(s) 105, 111, 202, 899
BveI ACCTGC 2 cut(s) 151, 157
Cac8I GCNNGC 3 cut(s) 129, 893, 897
CaiI CAGNNNCTG 2 cut(s) 269, 749
CfoI GCGC 1 cut(s) 1097
Cfr13I GGNCC 1 cut(s) 1185
Csp6I GTAC 4 cut(s) 30, 226, 1039, 1161
CviAII CATG 9 cut(s) 128, 440, 511, 545, 766, 939, 978, 1073, 1150
CviQI GTAC 4 cut(s) 30, 226, 1039, 1161
DdeI CTNAG 7 cut(s) 222, 450, 1013, 1128, 1164, 1287, 1309
DpnI GATC 7 cut(s) 351, 384, 624, 672, 738, 826, 1205
DpnII GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
Eam1104I CTCTTC 7 cut(s) 609, 687, 979, 1022, 1195, 1295, 1320
EarI CTCTTC 7 cut(s) 609, 687, 979, 1022, 1195, 1295, 1320
Eco130I CCWWGG 2 cut(s) 765, 1334
Eco147I AGGCCT 1 cut(s) 1333
Eco24I GRGCYC 1 cut(s) 1363
Eco57I CTGAAG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 1185
EcoRI GAATTC 2 cut(s) 752, 1003
EcoRII CCWGG 2 cut(s) 960, 1293
EcoT14I CCWWGG 2 cut(s) 765, 1334
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 1 cut(s) 1363
ErhI CCWWGG 2 cut(s) 765, 1334
FaeI CATG 9 cut(s) 131, 443, 514, 548, 769, 942, 981, 1076, 1153
FalI AAGNNNNNCTT 2 cut(s) 1174, 1206
FaqI GGGAC 1 cut(s) 1065
FatI CATG 9 cut(s) 127, 439, 510, 544, 765, 938, 977, 1072, 1149
FauNDI CATATG 1 cut(s) 887
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 642, 1234
FokI GGATG 3 cut(s) 401, 503, 1070
FriOI GRGCYC 1 cut(s) 1363
Fsp4HI GCNGC 2 cut(s) 642, 1234
GlaI GCGC 1 cut(s) 1096
GluI GCNGC 2 cut(s) 642, 1234
GsuI CTGGAG 1 cut(s) 820
HaeII RGCGCY 1 cut(s) 1098
HaeIII GGCC 2 cut(s) 1186, 1333
HapII CCGG 2 cut(s) 287, 411
HhaI GCGC 1 cut(s) 1097
Hin1II CATG 9 cut(s) 131, 443, 514, 548, 769, 942, 981, 1076, 1153
Hin6I GCGC 1 cut(s) 1095
HinP1I GCGC 1 cut(s) 1095
HincII GTYRAC 1 cut(s) 1112
HindII GTYRAC 1 cut(s) 1112
HindIII AAGCTT 1 cut(s) 45
HpaII CCGG 2 cut(s) 287, 411
HphI GGTGA 2 cut(s) 710, 781
Hpy166II GTNNAC 2 cut(s) 239, 1112
Hpy188I TCNGA 8 cut(s) 256, 271, 304, 598, 745, 824, 1225, 1288
Hpy188III TCNNGA 5 cut(s) 8, 386, 929, 1311, 1408
Hpy8I GTNNAC 2 cut(s) 239, 1112
HpyAV CCTTC 6 cut(s) 206, 210, 388, 517, 613, 727
HpyCH4III ACNGT 2 cut(s) 115, 1247
HpyCH4IV ACGT 2 cut(s) 228, 471
HpyCH4V TGCA 9 cut(s) 98, 127, 166, 195, 499, 803, 923, 1024, 1061
HpyF3I CTNAG 7 cut(s) 222, 450, 1013, 1128, 1164, 1287, 1309
HpySE526I ACGT 2 cut(s) 228, 471
Hsp92II CATG 9 cut(s) 131, 443, 514, 548, 769, 942, 981, 1076, 1153
HspAI GCGC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
LmnI GCTCC 4 cut(s) 413, 430, 638, 1344
Lsp1109I GCAGC 2 cut(s) 653, 1245
LweI GCATC 4 cut(s) 442, 481, 1048, 1070
MaeII ACGT 2 cut(s) 228, 471
MaeIII GTNAC 4 cut(s) 109, 481, 627, 1304
MalI GATC 7 cut(s) 351, 384, 624, 672, 738, 826, 1205
MboI GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
MfeI CAATTG 1 cut(s) 1389
MflI RGATCY 3 cut(s) 382, 736, 824
MhlI GDGCHC 1 cut(s) 1363
MluCI AATT 9 cut(s) 752, 783, 914, 1003, 1198, 1274, 1389, 1411, 1434
MlyI GAGTC 7 cut(s) 20, 113, 128, 728, 1220, 1301, 1342
Mph1103I ATGCAT 1 cut(s) 1063
MseI TTAA 5 cut(s) 36, 171, 378, 818, 990
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 2 cut(s) 644, 1103
MspI CCGG 2 cut(s) 287, 411
MspR9I CCNGG 2 cut(s) 962, 1295
MunI CAATTG 1 cut(s) 1389
MvaI CCWGG 2 cut(s) 962, 1295
NcoI CCATGG 1 cut(s) 765
NdeI CATATG 1 cut(s) 887
NdeII GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
NlaIII CATG 9 cut(s) 131, 443, 514, 548, 769, 942, 981, 1076, 1153
NlaIV GGNNCC 2 cut(s) 826, 1340
NmuCI GTSAC 4 cut(s) 109, 481, 627, 1304
NsiI ATGCAT 1 cut(s) 1063
NspI RCATGY 2 cut(s) 131, 514
NspV TTCGAA 1 cut(s) 521
PaeI GCATGC 1 cut(s) 131
PaqCI CACCTGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 1333
PciI ACATGT 1 cut(s) 510
PfeI GAWTC 6 cut(s) 4, 61, 454, 605, 871, 1174
PfoI TCCNGGA 1 cut(s) 960
PkrI GCNGC 2 cut(s) 643, 1235
PleI GAGTC 7 cut(s) 19, 112, 127, 727, 1219, 1301, 1342
PpsI GAGTC 7 cut(s) 19, 112, 127, 727, 1219, 1301, 1342
PscI ACATGT 1 cut(s) 510
PshBI ATTAAT 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 960, 1293
PspGI CCWGG 2 cut(s) 960, 1293
PspN4I GGNNCC 2 cut(s) 826, 1340
PspPI GGNCC 1 cut(s) 1185
PsrI GAACNNNNNNTAC 2 cut(s) 1235, 1267
PstNI CAGNNNCTG 2 cut(s) 269, 749
PsuI RGATCY 3 cut(s) 382, 736, 824
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 4 cut(s) 31, 227, 1040, 1162
RsaNI GTAC 4 cut(s) 30, 226, 1039, 1161
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 5 cut(s) 36, 171, 378, 818, 990
SatI GCNGC 2 cut(s) 642, 1234
Sau3AI GATC 7 cut(s) 349, 382, 622, 670, 736, 824, 1203
Sau96I GGNCC 1 cut(s) 1185
SchI GAGTC 7 cut(s) 20, 113, 128, 728, 1220, 1301, 1342
ScrFI CCNGG 2 cut(s) 962, 1295
SduI GDGCHC 1 cut(s) 1363
SfaNI GCATC 4 cut(s) 442, 481, 1048, 1070
SfcI CTRYAG 1 cut(s) 404
SfuI TTCGAA 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 438
SphI GCATGC 1 cut(s) 131
Sse9I AATT 9 cut(s) 752, 783, 914, 1003, 1198, 1274, 1389, 1411, 1434
SseBI AGGCCT 1 cut(s) 1333
SsiI CCGC 2 cut(s) 1042, 1101
StuI AGGCCT 1 cut(s) 1333
StyD4I CCNGG 2 cut(s) 960, 1293
StyI CCWWGG 2 cut(s) 765, 1334
TaaI ACNGT 2 cut(s) 115, 1247
TaiI ACGT 2 cut(s) 231, 474
TaqI TCGA 2 cut(s) 24, 521
TasI AATT 9 cut(s) 752, 783, 914, 1003, 1198, 1274, 1389, 1411, 1434
TfiI GAWTC 6 cut(s) 4, 61, 454, 605, 871, 1174
Tru1I TTAA 5 cut(s) 36, 171, 378, 818, 990
Tru9I TTAA 5 cut(s) 36, 171, 378, 818, 990
TscAI CASTG 4 cut(s) 105, 118, 202, 906
TseFI GTSAC 4 cut(s) 109, 481, 627, 1304
TseI GCWGC 2 cut(s) 641, 1233
Tsp45I GTSAC 4 cut(s) 109, 481, 627, 1304
TspRI CASTG 4 cut(s) 105, 118, 202, 906
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 752, 1003
XceI RCATGY 2 cut(s) 131, 514
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.