FvH4_2g05722

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017978
Physical Location & Seq
Forward (+)
4683370 .. 4685023
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05722.t1

Sequence Viewer

Length: 765 bp
ATGAAGTACAAAAACGGCGAGAATATATGTACCCAGGTCCCTGATTTGAAGGTCGTAGATCTCAGTAGCACTAGTATTTCGATATTACCACACTCTTTAAGTCTGCGGACCAACCTCCAGGCACTGTATTTAGATGGTTGCCGTTCATTAAGGAGTGTTTCTGTTCTAGGAGAACTGAAGAAACTTGAAATCCTTAGCCTGAGAGATTCAGCCCTTGAGGAATTACCTGTAGTAATAGGAAATCTGGTTAAGCTAAGAATGCTTGATGTTAGTGGGAGTCGGTACATTAAGAGAATTCCATTGAACTTGATATCAAGGTTGTCTAGGTTAGAAGAGTTGTACATGCAAGATAGTTTTCGGGAGTGGGGGAGTAGAGTTGAGGGAGAAGGAGAAGAGACCAATGTTGGATTTGATGAGTTGAGTGGCCTGTCATACTTGAACATTTTGGAGGTTGACATAACTGATGCAGAATGCTTGCCTCACAATGTTAGCTGCATTCCCAAATGGGGAAAGTTTAACATATGTATCAGAGGAAACAGTCGTTGGTCCTCGGAAAGCTACCTTTTGAATGAAGTTTTAGAGAGTCATAACAAGTTGATTGTTCTTCCAGAGCTCACAAGCTTCTACACCGGAAATAACCGGATCGAGTGTCCCTCATTGCAAGTGTTAAATGTGTTCAGTTGCCCTCGATTTCCAACCTCTACTAATGACTTCCACAGCAGGAACCCAGTTACAATTGAAGCTCGACATACATCATCAGCTTGA

Protein Analysis

255

Amino Acids

28.65

Weight (kDa)

5.8

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 21 - 233 1.6e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 106
AclWI GGATC 1 cut(s) 650
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 4 cut(s) 8, 31, 284, 341
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 6 cut(s) 49, 188, 304, 439, 568, 740
AhlI ACTAGT 1 cut(s) 71
AjnI CCWGG 2 cut(s) 33, 117
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AluBI AGCT 7 cut(s) 253, 492, 558, 613, 621, 743, 761
AluI AGCT 7 cut(s) 253, 492, 558, 613, 621, 743, 761
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 1 cut(s) 650
AlwNI CAGNNNCTG 1 cut(s) 124
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 1 cut(s) 492
ApoI RAATTY 1 cut(s) 294
AspS9I GGNCC 3 cut(s) 37, 108, 546
AvaII GGWCC 3 cut(s) 37, 108, 546
BaeI ACNNNNGTAYC 2 cut(s) 508, 541
BanII GRGCYC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 479
BccI CCATC 1 cut(s) 128
BceAI ACGGC 2 cut(s) 31, 126
BciT130I CCWGG 2 cut(s) 35, 119
BcoDI GTCTC 1 cut(s) 389
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 3 cut(s) 72, 167, 324
BfmI CTRYAG 1 cut(s) 228
BglII AGATCT 1 cut(s) 58
BisI GCNGC 1 cut(s) 493
BlsI GCNGC 1 cut(s) 494
Bme1390I CCNGG 2 cut(s) 35, 119
Bme18I GGWCC 3 cut(s) 37, 108, 546
BmgT120I GGNCC 3 cut(s) 37, 108, 546
BmiI GGNNCC 2 cut(s) 39, 725
BmrFI CCNGG 2 cut(s) 35, 119
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 722
BplI GAGNNNNNCTC 2 cut(s) 638, 670
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 33, 549
BsaWI WCCGGW 2 cut(s) 629, 639
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse1I ACTGG 1 cut(s) 728
Bse3DI GCAATG 1 cut(s) 656
BseBI CCWGG 2 cut(s) 35, 119
BseDI CCNNGG 2 cut(s) 33, 549
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 2 cut(s) 76, 191
BseNI ACTGG 1 cut(s) 728
BseXI GCAGC 1 cut(s) 479
BshFI GGCC 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 630, 640
BslFI GGGAC 2 cut(s) 23, 636
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 23, 636
BsmI GAATGC 3 cut(s) 264, 476, 495
BsnI GGCC 1 cut(s) 426
Bso31I GGTCTC 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 615
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 58, 642
BspACI CCGC 1 cut(s) 106
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 2 cut(s) 75, 192
BspLI GGNNCC 2 cut(s) 39, 725
BspPI GGATC 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 656
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 1 cut(s) 728
BssECI CCNNGG 2 cut(s) 33, 549
BssMI GATC 2 cut(s) 58, 642
Bst2UI CCWGG 2 cut(s) 35, 119
Bst4CI ACNGT 2 cut(s) 126, 539
Bst6I CTCTTC 2 cut(s) 327, 387
BstAUI TGTACA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 4 cut(s) 62, 194, 200, 254
BstKTI GATC 2 cut(s) 61, 645
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 2 cut(s) 58, 642
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 2 cut(s) 35, 119
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 33, 117
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 122
Cac8I GCNNGC 1 cut(s) 476
CaiI CAGNNNCTG 1 cut(s) 124
Cfr13I GGNCC 3 cut(s) 37, 108, 546
Csp6I GTAC 4 cut(s) 7, 30, 283, 340
CviAII CATG 1 cut(s) 343
CviQI GTAC 4 cut(s) 7, 30, 283, 340
DdeI CTNAG 4 cut(s) 62, 194, 200, 254
DpnI GATC 2 cut(s) 60, 644
DpnII GATC 2 cut(s) 58, 642
Eam1104I CTCTTC 2 cut(s) 327, 387
EarI CTCTTC 2 cut(s) 327, 387
Ecl136II GAGCTC 1 cut(s) 613
Eco24I GRGCYC 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 3 cut(s) 37, 108, 546
Eco53kI GAGCTC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 33, 117
EcoRV GATATC 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 615
FaeI CATG 1 cut(s) 346
FaiI YATR 9 cut(s) 26, 28, 344, 433, 458, 521, 523, 588, 750
FaqI GGGAC 2 cut(s) 23, 636
FatI CATG 1 cut(s) 342
FauNDI CATATG 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 1 cut(s) 493
FspBI CTAG 3 cut(s) 72, 167, 324
GluI GCNGC 1 cut(s) 493
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 1 cut(s) 426
HapII CCGG 2 cut(s) 630, 640
Hin1II CATG 1 cut(s) 346
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 3 cut(s) 206, 277, 583
HpaII CCGG 2 cut(s) 630, 640
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 2 cut(s) 530, 553
Hpy188III TCNNGA 2 cut(s) 359, 608
Hpy8I GTNNAC 1 cut(s) 454
HpyAV CCTTC 2 cut(s) 43, 380
HpyCH4III ACNGT 2 cut(s) 126, 539
HpyCH4V TGCA 4 cut(s) 346, 467, 495, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 4 cut(s) 62, 194, 200, 254
Hsp92II CATG 1 cut(s) 346
Kzo9I GATC 2 cut(s) 58, 642
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 1 cut(s) 454
MaeI CTAG 3 cut(s) 72, 167, 324
MaeIII GTNAC 1 cut(s) 730
MalI GATC 2 cut(s) 60, 644
MboI GATC 2 cut(s) 58, 642
MboII GAAGA 4 cut(s) 190, 344, 404, 596
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 615
MluCI AATT 3 cut(s) 221, 294, 735
MlyI GAGTC 2 cut(s) 286, 592
MmeI TCCRAC 2 cut(s) 385, 719
MseI TTAA 6 cut(s) 98, 149, 249, 288, 516, 668
MspI CCGG 2 cut(s) 630, 640
MspR9I CCNGG 2 cut(s) 35, 119
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 3 cut(s) 264, 476, 495
MvaI CCWGG 2 cut(s) 35, 119
MwoI GCNNNNNNNGC 1 cut(s) 259
NdeI CATATG 1 cut(s) 521
NdeII GATC 2 cut(s) 58, 642
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 2 cut(s) 39, 725
NspI RCATGY 1 cut(s) 346
PctI GAATGC 3 cut(s) 264, 476, 495
PfeI GAWTC 1 cut(s) 206
PkrI GCNGC 1 cut(s) 494
PleI GAGTC 2 cut(s) 285, 591
PpsI GAGTC 2 cut(s) 285, 591
PpuMI RGGWCCY 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 2 cut(s) 33, 117
PspGI CCWGG 2 cut(s) 33, 117
PspN4I GGNNCC 2 cut(s) 39, 725
PspPI GGNCC 3 cut(s) 37, 108, 546
PspPPI RGGWCCY 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 4 cut(s) 8, 31, 284, 341
RsaNI GTAC 4 cut(s) 7, 30, 283, 340
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 6 cut(s) 98, 149, 249, 288, 516, 668
SatI GCNGC 1 cut(s) 493
Sau3AI GATC 2 cut(s) 58, 642
Sau96I GGNCC 3 cut(s) 37, 108, 546
SchI GAGTC 2 cut(s) 286, 592
ScrFI CCNGG 2 cut(s) 35, 119
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 3 cut(s) 37, 108, 546
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 71
Sse9I AATT 3 cut(s) 221, 294, 735
SsiI CCGC 1 cut(s) 106
SspMI CTAG 3 cut(s) 72, 167, 324
SstI GAGCTC 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 33, 117
TaaI ACNGT 2 cut(s) 126, 539
TaqI TCGA 4 cut(s) 80, 645, 688, 745
TasI AATT 3 cut(s) 221, 294, 735
TatI WGTACW 2 cut(s) 6, 339
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 6 cut(s) 98, 149, 249, 288, 516, 668
Tru9I TTAA 6 cut(s) 98, 149, 249, 288, 516, 668
TscAI CASTG 1 cut(s) 129
TseI GCWGC 1 cut(s) 492
TspDTI ATGAA 3 cut(s) 17, 135, 585
TspRI CASTG 1 cut(s) 129
VpaK11BI GGWCC 3 cut(s) 37, 108, 546
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 346
XspI CTAG 3 cut(s) 72, 167, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.