Rroxscaffold_3G00238020

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017978
Physical Location & Seq
Forward (+)
25974406 .. 25975100
695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00238020.1

Sequence Viewer

Length: 450 bp
ATGGTGATTGGAGCTCTCACAGCGCACGGGACCCGCAAGGTTCAGCAAGCGAAGTTCTTGAGTGGAGCTAGCGATCGAGTTGAGCTTGCTCATGTCTTAGATCTCGGTTGCACTTGGTATTTCAATCTTACCGTCTTTGAGTCGGTGACCAATCTCCAGGGCATTATATTTAGACATTGCTGGTCAATAAGGGATGTATCTCGACTAGGTGAATTGAAGAAACTTGAAATTCTTAGCCCGAGATATTTAGCCCTTGGGGAATTACCCGAAGAAATAGGAAATTTGGCTAAGCTAAGAATGCTTGATCTTAGTTGGAGTCGGTACATTAAGAGAATTCTATCCACTTTGATATCAAGGTTGTCTAGGTTGGAAGAACTATACATGCAAAATAGTTTTCGGACTGGGAGAGTAAGGTTGAGCGAGAAGGAGAAGAAACCAAGGTTAGATTAA

Protein Analysis

149

Amino Acids

17.22

Weight (kDa)

9.82

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 52 - 133 9.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 34
AcsI RAATTY 3 cut(s) 228, 280, 333
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 3 cut(s) 124, 217, 227
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 4 cut(s) 14, 68, 85, 292
AluI AGCT 4 cut(s) 14, 68, 85, 292
Alw21I GWGCWC 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 238
ApoI RAATTY 3 cut(s) 228, 280, 333
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 30
AsuHPI GGTGA 3 cut(s) 16, 157, 221
AsuNHI GCTAGC 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 158
BfaI CTAG 3 cut(s) 69, 206, 363
BglII AGATCT 1 cut(s) 100
BlpI GCTNAGC 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 2 cut(s) 31, 32
BmrFI CCNGG 1 cut(s) 158
BmrI ACTGGG 1 cut(s) 411
BmtI GCTAGC 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 157, 253, 437
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 3 cut(s) 157, 253, 437
BseGI GGATG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 76
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 16
BsiHKCI CYCGRG 1 cut(s) 238
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 303
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 3 cut(s) 73, 100, 304
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 31, 32
BspOI GCTAGC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 157, 253, 437
BssMI GATC 3 cut(s) 73, 100, 304
BssT1I CCWWGG 2 cut(s) 253, 437
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 48, 70, 87
BstDEI CTNAG 5 cut(s) 97, 233, 288, 293, 308
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 1 cut(s) 199
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 3 cut(s) 76, 103, 307
BstMBI GATC 3 cut(s) 73, 100, 304
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 2 cut(s) 20, 298
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 1 cut(s) 385
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BtsCI GGATG 1 cut(s) 199
Cac8I GCNNGC 3 cut(s) 48, 70, 87
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 92, 382
CviJI RGCY 7 cut(s) 14, 68, 85, 237, 251, 287, 292
CviKI_1 RGCY 7 cut(s) 14, 68, 85, 237, 251, 287, 292
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 5 cut(s) 97, 233, 288, 293, 308
DpnI GATC 3 cut(s) 75, 102, 306
DpnII GATC 3 cut(s) 73, 100, 304
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 253, 437
Eco24I GRGCYC 1 cut(s) 16
Eco32I GATATC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 238
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 2 cut(s) 253, 437
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 253, 437
FaeI CATG 2 cut(s) 95, 385
FaiI YATR 4 cut(s) 93, 167, 379, 383
FaqI GGGAC 1 cut(s) 43
FatI CATG 2 cut(s) 91, 381
FauI CCCGC 1 cut(s) 41
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 3 cut(s) 69, 206, 363
GlaI GCGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 140
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 95, 385
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 2 cut(s) 140, 316
HphI GGTGA 3 cut(s) 16, 157, 221
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 2 cut(s) 58, 201
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 111, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 298
HpyF3I CTNAG 5 cut(s) 97, 233, 288, 293, 308
Hsp92II CATG 2 cut(s) 95, 385
HspAI GCGC 1 cut(s) 23
KflI GGGWCCC 1 cut(s) 30
Kzo9I GATC 3 cut(s) 73, 100, 304
LmnI GCTCC 2 cut(s) 11, 65
LpnPI CCDG 4 cut(s) 143, 166, 170, 387
MaeI CTAG 3 cut(s) 69, 206, 363
MaeIII GTNAC 1 cut(s) 145
MalI GATC 3 cut(s) 75, 102, 306
MboI GATC 3 cut(s) 73, 100, 304
MboII GAAGA 4 cut(s) 229, 281, 383, 442
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 5 cut(s) 212, 228, 260, 280, 333
MlyI GAGTC 2 cut(s) 149, 325
MmeI TCCRAC 2 cut(s) 293, 348
MseI TTAA 2 cut(s) 327, 448
MspR9I CCNGG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 20, 298
NdeII GATC 3 cut(s) 73, 100, 304
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 95, 385
NlaIV GGNNCC 2 cut(s) 31, 32
NmuCI GTSAC 1 cut(s) 145
NspI RCATGY 1 cut(s) 385
PctI GAATGC 1 cut(s) 303
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 2 cut(s) 148, 324
PpsI GAGTC 2 cut(s) 148, 324
PpuMI RGGWCCY 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 156
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 31, 32
PspPI GGNCC 1 cut(s) 30
PspPPI RGGWCCY 1 cut(s) 30
PsuI RGATCY 1 cut(s) 100
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SacI GAGCTC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 327, 448
Sau3AI GATC 3 cut(s) 73, 100, 304
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 2 cut(s) 149, 325
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 16
SinI GGWCC 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 5 cut(s) 212, 228, 260, 280, 333
SsiI CCGC 1 cut(s) 34
SspMI CTAG 3 cut(s) 69, 206, 363
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 2 cut(s) 253, 437
TaaI ACNGT 1 cut(s) 133
TaqI TCGA 2 cut(s) 76, 202
TasI AATT 5 cut(s) 212, 228, 260, 280, 333
Tru1I TTAA 2 cut(s) 327, 448
Tru9I TTAA 2 cut(s) 327, 448
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 30
XapI RAATTY 3 cut(s) 228, 280, 333
XceI RCATGY 1 cut(s) 385
XspI CTAG 3 cut(s) 69, 206, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.