FvH4_2g06130

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014154
Physical Location & Seq
Forward (+)
5097993 .. 5101285
3293 bp
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UTR
Exon/CDS
Intron
FvH4_2g06130.t1

Sequence Viewer

Length: 528 bp
ATGTGGGCTACCAACCCTAGCCTCTTCTTCCCCTCCAAACCCTTCTCCCTCCGCTGCCGCGCCGCTGCCGGCGACCCTCCTCGGACCGCCGCATTCCCTCGCCTGATCCAGTTCCCCTTCGCCGCCTCCGACGTCGCCTCCGTCAGCGGCCGAATCGGCTTCGACACCGAACTCGACGGCGGCGCGGCGAGACCTCGCGGCGGAAGCTCCGGCGGTGGTACTAAGGTGACGGCGAAGGAGCCCAAGTGGTCGCGGGATAGAGAGAGTTACTTGGTGGACGATGGAGACGCGCTGCCTCTCCCCATGACTTATCCAGATACGGCTCCGGTGACGCCGGAGGTCGTTGACCAGAGGCTCCGGTGCGACCCTGTAGTTGAGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGCAGGAGTTGCCAAGGCTCAGGATATGCTAGTTATTATAACAAAAGAGGGAAGCAAAATGTATGCAAATGCATACCTTGCCAAGGAATCGCCAGGACCACGGATAATGTATAG

Protein Analysis

176

Amino Acids

18.99

Weight (kDa)

8.63

Isoelectric Point (pI)

31.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AatII GACGTC 1 cut(s) 135
AccII CGCG 5 cut(s) 60, 185, 198, 253, 290
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 148
AcyI GRCGYC 2 cut(s) 132, 332
AfaI GTAC 1 cut(s) 220
AfiI CCNNNNNNNGG 2 cut(s) 200, 497
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 1 cut(s) 207
AluI AGCT 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 184, 279
AlwI GGATC 1 cut(s) 100
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 3 cut(s) 54, 65, 292
AspLEI GCGC 3 cut(s) 62, 185, 292
AspS9I GGNCC 2 cut(s) 84, 510
AsuHPI GGTGA 2 cut(s) 238, 340
AvaII GGWCC 2 cut(s) 84, 510
BaeI ACNNNNGTAYC 2 cut(s) 210, 243
BanII GRGCYC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 41, 52, 279
BccI CCATC 1 cut(s) 275
BceAI ACGGC 3 cut(s) 193, 246, 336
BciT130I CCWGG 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 184, 279
BfaI CTAG 2 cut(s) 18, 444
BfmI CTRYAG 1 cut(s) 369
BglI GCCNNNNNGGC 1 cut(s) 156
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 2 cut(s) 84, 510
BmgT120I GGNCC 2 cut(s) 84, 510
BmiI GGNNCC 3 cut(s) 240, 324, 356
BmrFI CCNGG 1 cut(s) 508
Bpu10I CCTNAGC 1 cut(s) 433
BsaHI GRCGYC 2 cut(s) 132, 332
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 4 cut(s) 80, 427, 496, 513
BsaWI WCCGGW 2 cut(s) 325, 357
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 497
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 4 cut(s) 80, 427, 496, 513
BseLI CCNNNNNNNGG 2 cut(s) 200, 497
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 69
BseX3I CGGCCG 1 cut(s) 148
BseXI GCAGC 3 cut(s) 41, 52, 279
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 5 cut(s) 60, 185, 198, 253, 290
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 151
BsiSI CCGG 5 cut(s) 69, 210, 326, 335, 358
BslI CCNNNNNNNGG 2 cut(s) 200, 497
BsmAI GTCTC 2 cut(s) 184, 279
BsmBI CGTCTC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 1 cut(s) 150
Bso31I GGTCTC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 1 cut(s) 105
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 446
BspFNI CGCG 5 cut(s) 60, 185, 198, 253, 290
BspLI GGNNCC 3 cut(s) 240, 324, 356
BspPI GGATC 1 cut(s) 100
BspTNI GGTCTC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 80, 427, 496, 513
BssMI GATC 1 cut(s) 105
BssNI GRCGYC 2 cut(s) 132, 332
BssT1I CCWWGG 2 cut(s) 427, 496
Bst2UI CCWGG 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 2 cut(s) 132, 332
BstAPI GCANNNNNTGC 2 cut(s) 423, 492
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 2 cut(s) 222, 433
BstDSI CCRYGG 1 cut(s) 513
BstENI CCTNNNNNAGG 1 cut(s) 495
BstFNI CGCG 5 cut(s) 60, 185, 198, 253, 290
BstHHI GCGC 3 cut(s) 62, 185, 292
BstKTI GATC 1 cut(s) 108
BstMAI GTCTC 2 cut(s) 184, 279
BstMBI GATC 1 cut(s) 105
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 4 cut(s) 156, 204, 423, 492
BstNI CCWGG 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 369
BstUI CGCG 5 cut(s) 60, 185, 198, 253, 290
BstV1I GCAGC 3 cut(s) 41, 52, 279
BstZI CGGCCG 1 cut(s) 148
BsuRI GGCC 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 70
CfoI GCGC 3 cut(s) 62, 185, 292
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 84, 510
CpoI CGGWCCG 1 cut(s) 84
CseI GACGC 2 cut(s) 296, 340
Csp6I GTAC 1 cut(s) 219
CspI CGGWCCG 1 cut(s) 84
CviAII CATG 1 cut(s) 304
CviJI RGCY 9 cut(s) 8, 21, 150, 159, 207, 241, 323, 355, 432
CviKI_1 RGCY 9 cut(s) 8, 21, 150, 159, 207, 241, 323, 355, 432
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 2 cut(s) 222, 433
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 148
EagI CGGCCG 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 216
EclXI CGGCCG 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 427, 496
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 84, 510
Eco52I CGGCCG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 2 cut(s) 427, 496
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 2 cut(s) 427, 496
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 1 cut(s) 307
FaiI YATR 7 cut(s) 305, 399, 441, 453, 478, 488, 526
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 243
FspBI CTAG 2 cut(s) 18, 444
GlaI GCGC 3 cut(s) 61, 184, 291
HaeIII GGCC 1 cut(s) 150
HapII CCGG 5 cut(s) 69, 210, 326, 335, 358
HgaI GACGC 2 cut(s) 296, 340
HhaI GCGC 3 cut(s) 62, 185, 292
Hin1I GRCGYC 2 cut(s) 132, 332
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 3 cut(s) 60, 183, 290
HinP1I GCGC 3 cut(s) 60, 183, 290
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 153, 501
HpaII CCGG 5 cut(s) 69, 210, 326, 335, 358
HphI GGTGA 2 cut(s) 238, 340
Hpy166II GTNNAC 2 cut(s) 277, 346
Hpy188I TCNGA 2 cut(s) 84, 130
Hpy188III TCNNGA 2 cut(s) 314, 435
Hpy8I GTNNAC 2 cut(s) 277, 346
Hpy99I CGWCG 3 cut(s) 134, 137, 179
HpyAV CCTTC 3 cut(s) 52, 127, 229
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 384, 417, 480, 486
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 204, 423, 492
HpyF3I CTNAG 2 cut(s) 222, 433
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 132, 332
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 3 cut(s) 60, 183, 290
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 105
LmnI GCTCC 4 cut(s) 212, 238, 328, 360
Lsp1109I GCAGC 3 cut(s) 41, 52, 279
MaeI CTAG 2 cut(s) 18, 444
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 226, 266, 328
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 2 cut(s) 16, 19
MhlI GDGCHC 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 153
Mph1103I ATGCAT 1 cut(s) 488
MroNI GCCGGC 1 cut(s) 68
MspA1I CMGCKG 3 cut(s) 54, 65, 147
MspI CCGG 5 cut(s) 69, 210, 326, 335, 358
MspR9I CCNGG 1 cut(s) 508
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 508
MvnI CGCG 5 cut(s) 60, 185, 198, 253, 290
MwoI GCNNNNNNNGC 4 cut(s) 156, 204, 423, 492
NaeI GCCGGC 1 cut(s) 70
NdeII GATC 1 cut(s) 105
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 3 cut(s) 240, 324, 356
NmuCI GTSAC 2 cut(s) 226, 328
NsiI ATGCAT 1 cut(s) 488
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 138
PctI GAATGC 1 cut(s) 92
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 153, 501
PsiI TTATAA 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 506
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 3 cut(s) 240, 324, 356
PspPI GGNCC 2 cut(s) 84, 510
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
Sau3AI GATC 1 cut(s) 105
Sau96I GGNCC 2 cut(s) 84, 510
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 1 cut(s) 243
SetI ASST 6 cut(s) 135, 196, 209, 228, 342, 493
SfcI CTRYAG 1 cut(s) 369
SinI GGWCC 2 cut(s) 84, 510
SspMI CTAG 2 cut(s) 18, 444
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 2 cut(s) 427, 496
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 162, 174
TauI GCSGC 8 cut(s) 60, 65, 92, 125, 150, 183, 188, 201
TfiI GAWTC 2 cut(s) 153, 501
TseFI GTSAC 2 cut(s) 226, 328
TseI GCWGC 3 cut(s) 54, 65, 292
Tsp45I GTSAC 2 cut(s) 226, 328
TspDTI ATGAA 1 cut(s) 414
TspGWI ACGGA 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 84, 510
XagI CCTNNNNNAGG 1 cut(s) 495
XspI CTAG 2 cut(s) 18, 444
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.