Rmu_sc0000659.1_g000009

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000659.1
OG0014154
Physical Location & Seq
Forward (+)
38082 .. 40675
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000659.1_g000009.1.cds

Sequence Viewer

Length: 336 bp
atgaagataggagcttgctgctcttatcgtccgcgtgacgtcagttttcttggagattcttggagagtctacccaccctcaagaagtcagttttccagcaagtccatcagagacaaacgacaccgtcataaaaaggattgcaagatagtagtgtatgaatggacaggaaagtgtaggagttgccagggctcaggatacgctagttattataacaaaagagggaaggaaaatgtctgcaaatgcataccttgccaaggaatcggatatgtacaaaagataacaactcgcaccaacattgatgtaatggaagatttggataacggaaaaccaccatga

Protein Analysis

111

Amino Acids

12.76

Weight (kDa)

9.53

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
AatII GACGTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 34
AciI CCGC 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 254
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 18
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BanII GRGCYC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 5
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 185
BciVI GTATCC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 1 cut(s) 201
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 184, 253
Bsc4I CCNNNNNNNGG 1 cut(s) 254
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 184, 253
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 268
BspACI CCGC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 184, 253
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 249
BstAUI TGTACA 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 190
BstENI CCTNNNNNAGG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 105
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstUI CGCG 1 cut(s) 34
BstV1I GCAGC 1 cut(s) 5
BsuI GTATCC 1 cut(s) 188
Cac8I GCNNGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 269
CviAII CATG 1 cut(s) 333
CviJI RGCY 2 cut(s) 14, 189
CviKI_1 RGCY 2 cut(s) 14, 189
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 1 cut(s) 336
FaiI YATR 6 cut(s) 129, 156, 210, 245, 267, 334
FatI CATG 1 cut(s) 332
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 19
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 201
GluI GCNGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 1 cut(s) 336
HinfI GANTC 3 cut(s) 56, 66, 258
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 110, 263
Hpy188III TCNNGA 2 cut(s) 81, 192
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 141, 237, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 1 cut(s) 190
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 1 cut(s) 336
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 5 cut(s) 109, 150, 170, 177, 197
Lsp1109I GCAGC 1 cut(s) 5
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 35
MboII GAAGA 2 cut(s) 16, 320
MhlI GDGCHC 1 cut(s) 191
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 2 cut(s) 88, 212
Mph1103I ATGCAT 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 34
MwoI GCNNNNNNNGC 1 cut(s) 249
NlaIII CATG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 245
PfeI GAWTC 2 cut(s) 56, 258
PflFI GACNNNGTC 1 cut(s) 123
PkrI GCNGC 1 cut(s) 20
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PsyI GACNNNGTC 1 cut(s) 123
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
SatI GCNGC 1 cut(s) 19
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 191
SetI ASST 3 cut(s) 16, 42, 250
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 1 cut(s) 42
TatI WGTACW 1 cut(s) 268
TfiI GAWTC 2 cut(s) 56, 258
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 17, 171
TspGWI ACGGA 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 123
XagI CCTNNNNNAGG 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 201
ZraI GACGTC 1 cut(s) 40
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.