Rw6G008040

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014154
Physical Location & Seq
Forward (+)
13663121 .. 13665394
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008040.1

Sequence Viewer

Length: 600 bp
ATGTTGGCTACTAACCCTAGCCTCTTCTTCCCCTCCAAACCCTCCATTGCCCTCCGCTGCCGCGCCGCCGCCGGCGACCCACCTCGAGGCGCCGCATTCCCTCGCCTGGTCCAGTTTCCCTTCGCCGCCTCCGACGTCGCCTCCGTCAGCGGCCGAATCGGCCTTGACACCGAACCCGACGGCGGCGGCGCGGCTCGGCTCCGCGGCGGAAGCTCCGGCGGTGCTATTAAGGTGACGGCGAAGGAGCCGAAGTGGTCGAGGGATAGAGAGAGTTACTTGGTCGACGATGGAGACGCGCTGCCTCTCCCCATGACTTATCCAGATACGGCTCCGGTGACGCCGGAGGTGATTGACCAGAGGCTCCGGTGTGACCCTGTAGTCGAGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGTAGGAGTTGCCAGGGCTCAGGATACGCTAGTTATTATAACAAAAGAGGGAAGGAAAATGTCTGCAAATGCATACCTTGCCAAGGAATCGGTAAGATGCTTGTCGATTTTTCTGAGGTTGTATTGTTGATGGATGAAATTATGGTTTTCTTACCTTGGTTTAGCTCAGGTGCATGTTAA

Protein Analysis

199

Amino Acids

21.45

Weight (kDa)

6.32

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AasI GACNNNNNNGTC 1 cut(s) 377
AatII GACGTC 1 cut(s) 138
AbsI CCTCGAGG 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 282
AccII CGCG 4 cut(s) 63, 191, 204, 296
AcoI YGGCCR 1 cut(s) 151
AcyI GRCGYC 3 cut(s) 90, 135, 338
AfiI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
AjnI CCWGG 2 cut(s) 105, 432
AluBI AGCT 2 cut(s) 213, 585
AluI AGCT 2 cut(s) 213, 585
Alw26I GTCTC 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 2 cut(s) 151, 160
ApeKI GCWGC 2 cut(s) 57, 298
AspLEI GCGC 4 cut(s) 65, 92, 191, 298
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 3 cut(s) 244, 346, 358
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 1 cut(s) 109
BaeI ACNNNNGTAYC 2 cut(s) 436, 469
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 44, 285
BccI CCATC 2 cut(s) 281, 544
BceAI ACGGC 3 cut(s) 196, 252, 342
BciT130I CCWGG 2 cut(s) 107, 434
BciVI GTATCC 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 2 cut(s) 18, 450
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 93
BfuI GTATCC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 107, 434
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 5 cut(s) 91, 200, 246, 330, 362
BmrFI CCNGG 2 cut(s) 107, 434
BmsI GCATC 1 cut(s) 507
Bpu10I CCTNAGC 2 cut(s) 439, 586
BsaHI GRCGYC 3 cut(s) 90, 135, 338
BsaJI CCNNGG 4 cut(s) 202, 433, 502, 575
BsaWI WCCGGW 2 cut(s) 331, 363
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 236, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 107, 434
BseDI CCNNGG 4 cut(s) 202, 433, 502, 575
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 453, 525, 600
BseNI ACTGG 1 cut(s) 112
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 44, 285
Bsh1236I CGCG 4 cut(s) 63, 191, 204, 296
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 153, 162
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 5 cut(s) 72, 216, 332, 341, 364
BslI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 503
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 153, 162
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 440
BspANI GGCC 2 cut(s) 153, 162
BspCNI CTCAG 3 cut(s) 452, 526, 599
BspFNI CGCG 4 cut(s) 63, 191, 204, 296
BspLI GGNNCC 5 cut(s) 91, 200, 246, 330, 362
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 4 cut(s) 202, 433, 502, 575
BssNI GRCGYC 3 cut(s) 90, 135, 338
BssT1I CCWWGG 2 cut(s) 502, 575
Bst2UI CCWGG 2 cut(s) 107, 434
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 3 cut(s) 90, 135, 338
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 3 cut(s) 439, 534, 586
BstDSI CCRYGG 1 cut(s) 202
BstENI CCTNNNNNAGG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 4 cut(s) 63, 191, 204, 296
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 4 cut(s) 65, 92, 191, 298
BstMAI GTCTC 1 cut(s) 285
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 159, 210, 498
BstNI CCWGG 2 cut(s) 107, 434
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 2 cut(s) 105, 432
BstSFI CTRYAG 1 cut(s) 375
BstUI CGCG 4 cut(s) 63, 191, 204, 296
BstV1I GCAGC 2 cut(s) 44, 285
BstZI CGGCCG 1 cut(s) 151
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 2 cut(s) 153, 162
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 4 cut(s) 65, 92, 191, 298
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 205
CseI GACGC 2 cut(s) 302, 346
CviAII CATG 2 cut(s) 310, 594
DdeI CTNAG 3 cut(s) 439, 534, 586
DinI GGCGCC 1 cut(s) 91
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 151
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 502, 575
Eco24I GRGCYC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 109
Eco52I CGGCCG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 105, 432
EcoT14I CCWWGG 2 cut(s) 502, 575
EcoT22I ATGCAT 1 cut(s) 494
EcoT38I GRGCYC 1 cut(s) 440
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 2 cut(s) 502, 575
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 2 cut(s) 313, 597
FaiI YATR 6 cut(s) 311, 405, 459, 494, 563, 595
FatI CATG 2 cut(s) 309, 593
FblI GTMKAC 1 cut(s) 282
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 440
FspBI CTAG 2 cut(s) 18, 450
GlaI GCGC 4 cut(s) 64, 91, 190, 297
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 153, 162
HapII CCGG 5 cut(s) 72, 216, 332, 341, 364
HgaI GACGC 2 cut(s) 302, 346
HhaI GCGC 4 cut(s) 65, 92, 191, 298
Hin1I GRCGYC 3 cut(s) 90, 135, 338
Hin1II CATG 2 cut(s) 313, 597
Hin6I GCGC 4 cut(s) 63, 90, 189, 296
HinP1I GCGC 4 cut(s) 63, 90, 189, 296
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 2 cut(s) 156, 507
HpaII CCGG 5 cut(s) 72, 216, 332, 341, 364
HphI GGTGA 3 cut(s) 244, 346, 358
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 133, 535
Hpy188III TCNNGA 2 cut(s) 320, 441
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 4 cut(s) 137, 140, 182, 287
HpyAV CCTTC 3 cut(s) 130, 235, 466
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 390, 486, 492, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 210, 498
HpyF3I CTNAG 3 cut(s) 439, 534, 586
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 3 cut(s) 90, 135, 338
Hsp92II CATG 2 cut(s) 313, 597
HspAI GCGC 4 cut(s) 63, 90, 189, 296
KasI GGCGCC 1 cut(s) 89
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
KspI CCGCGG 1 cut(s) 205
LmnI GCTCC 5 cut(s) 204, 218, 244, 334, 366
Lsp1109I GCAGC 2 cut(s) 44, 285
LweI GCATC 1 cut(s) 507
MaeI CTAG 2 cut(s) 18, 450
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 4 cut(s) 232, 272, 334, 368
MboII GAAGA 2 cut(s) 16, 19
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 1 cut(s) 494
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 2 cut(s) 228, 598
MspA1I CMGCKG 3 cut(s) 57, 150, 204
MspI CCGG 5 cut(s) 72, 216, 332, 341, 364
MspR9I CCNGG 2 cut(s) 107, 434
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 2 cut(s) 107, 434
MvnI CGCG 4 cut(s) 63, 191, 204, 296
MwoI GCNNNNNNNGC 3 cut(s) 159, 210, 498
NaeI GCCGGC 1 cut(s) 73
NarI GGCGCC 1 cut(s) 90
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 313, 597
NlaIV GGNNCC 5 cut(s) 91, 200, 246, 330, 362
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 3 cut(s) 232, 334, 368
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 1 cut(s) 597
PaeR7I CTCGAG 1 cut(s) 84
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 141
PctI GAATGC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 156, 507
PluTI GGCGCC 1 cut(s) 93
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 105, 432
PspGI CCWGG 2 cut(s) 105, 432
PspN4I GGNNCC 5 cut(s) 91, 200, 246, 330, 362
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 84
SacII CCGCGG 1 cut(s) 205
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 228, 598
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 107, 434
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 1 cut(s) 507
SfcI CTRYAG 1 cut(s) 375
SfiI GGCCNNNNNGGCC 1 cut(s) 159
SfoI GGCGCC 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 84
Sfr303I CCGCGG 1 cut(s) 205
SgrAI CRCCGGYG 1 cut(s) 71
SgrBI CCGCGG 1 cut(s) 205
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 1 cut(s) 558
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 2 cut(s) 18, 450
StyD4I CCNGG 2 cut(s) 105, 432
StyI CCWWGG 2 cut(s) 502, 575
TaiI ACGT 1 cut(s) 138
TaqI TCGA 5 cut(s) 85, 257, 282, 381, 525
TasI AATT 1 cut(s) 558
TfiI GAWTC 2 cut(s) 156, 507
Tru1I TTAA 2 cut(s) 228, 598
Tru9I TTAA 2 cut(s) 228, 598
TseFI GTSAC 3 cut(s) 232, 334, 368
TseI GCWGC 2 cut(s) 57, 298
Tsp45I GTSAC 3 cut(s) 232, 334, 368
TspDTI ATGAA 2 cut(s) 420, 570
TspGWI ACGGA 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 501
XceI RCATGY 1 cut(s) 597
XhoI CTCGAG 1 cut(s) 84
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 2 cut(s) 18, 450
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.