Rroxscaffold_7G00208470

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014154
Physical Location & Seq
Reverse (-)
58469093 .. 58472134
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208470.1

Sequence Viewer

Length: 588 bp
ATGTTGGCTACTAACCCTAGCCTCTTCTTCCCCTCCAAACCCTCCATTGCCCTCCGCTGCCGCGCCGCCGCCGGCGACCCTCCTCGAGGCGCCGCATTCCCTCGCCTGGTCCAGTTTCCCTTCGCCGCCTCCGACGTCGCCTCCGTCAGCGGCCGAATCGGCCTTGACACCGAACCCGACGGCGGCGGCGGCGCGGCTCGGCTTCGCGGCGGAAGCTCCGGCGGTGCTATTAAGGTGACGGCCAAGGAGCCGAAGTGGTCGAGGGATAGAGAGAGTTACTTGGTCGACGACGGAGACGCGCTGCCTCTCCCCATGACTTATCCAGATACGGCTCCGGTGACGCCGGAGGTGATTGACCAGAGGCTCCGGTGTGACCCTGTAGTCGAGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGTAGGAGTTGCCAGGGCTCAGGATATGCTAGTTATTATAACAAAAGAGGGAAGGAAAATGTCTGCAAATGCGTACCTTGCCAAGGAATCGGATATGTACAAAAGATAACAACTCGCACCAACATTGATGTAATGGAAGATTTGGATAACGGAAAACCACCATGA

Protein Analysis

195

Amino Acids

20.93

Weight (kDa)

8.08

Isoelectric Point (pI)

31.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19220
fragaria_vesca FvH4_2g06130 FvH4_2g06130
malus_domestica MD10G1094200.v1.1
prunus_persica Prupe.8G124000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257061
rosa_laevigata RLG00000014674
rosa_multiflora Rmu_sc0000659.1_g000009 Rmu_sc0038017.1_g000001 Rmu_sc0038018.1_g000001
rosa_roxburghii Rroxscaffold_7G00208470
rosa_rugosa Rorug05G0575500
rosa_samantha Rh6AG093500 Rh6BG084300 Rh6CG081000 Rh6DG076400
rosa_wichuraiana Rw6G008040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 462
AasI GACNNNNNNGTC 1 cut(s) 380
AatII GACGTC 1 cut(s) 138
AbsI CCTCGAGG 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 285
AccII CGCG 4 cut(s) 63, 194, 207, 299
AcoI YGGCCR 2 cut(s) 151, 240
AcyI GRCGYC 3 cut(s) 90, 135, 341
AfaI GTAC 2 cut(s) 498, 522
AfiI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 506
AjnI CCWGG 2 cut(s) 105, 435
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 288
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 3 cut(s) 151, 160, 240
ApeKI GCWGC 2 cut(s) 57, 301
AspLEI GCGC 4 cut(s) 65, 92, 194, 301
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 3 cut(s) 247, 349, 361
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 443
BbvI GCAGC 2 cut(s) 44, 288
BceAI ACGGC 3 cut(s) 196, 255, 345
BciT130I CCWGG 2 cut(s) 107, 437
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 2 cut(s) 18, 453
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 107, 437
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 4 cut(s) 91, 249, 333, 365
BmrFI CCNGG 2 cut(s) 107, 437
Bpu10I CCTNAGC 1 cut(s) 442
BsaHI GRCGYC 3 cut(s) 90, 135, 341
BsaJI CCNNGG 3 cut(s) 243, 436, 505
BsaWI WCCGGW 2 cut(s) 334, 366
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 239, 269
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 106, 182, 506
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 107, 437
BseDI CCNNGG 3 cut(s) 243, 436, 505
BseLI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 506
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 72
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 44, 288
Bsh1236I CGCG 4 cut(s) 63, 194, 207, 299
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 153, 162, 242
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 5 cut(s) 72, 219, 335, 344, 367
BslI CCNNNNNNNGG 4 cut(s) 86, 106, 182, 506
BsmAI GTCTC 1 cut(s) 288
BsmBI CGTCTC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 3 cut(s) 153, 162, 242
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 443
Bsp1407I TGTACA 1 cut(s) 520
BspANI GGCC 3 cut(s) 153, 162, 242
BspCNI CTCAG 1 cut(s) 455
BspFNI CGCG 4 cut(s) 63, 194, 207, 299
BspLI GGNNCC 4 cut(s) 91, 249, 333, 365
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 3 cut(s) 243, 436, 505
BssNI GRCGYC 3 cut(s) 90, 135, 341
BssT1I CCWWGG 2 cut(s) 243, 505
Bst2UI CCWGG 2 cut(s) 107, 437
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 3 cut(s) 90, 135, 341
BstAUI TGTACA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 442
BstENI CCTNNNNNAGG 2 cut(s) 84, 504
BstFNI CGCG 4 cut(s) 63, 194, 207, 299
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 4 cut(s) 65, 92, 194, 301
BstMAI GTCTC 1 cut(s) 288
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 4 cut(s) 159, 189, 213, 501
BstNI CCWGG 2 cut(s) 107, 437
BstSCI CCNGG 2 cut(s) 105, 435
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 4 cut(s) 63, 194, 207, 299
BstV1I GCAGC 2 cut(s) 44, 288
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 3 cut(s) 153, 162, 242
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 4 cut(s) 65, 92, 194, 301
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 2 cut(s) 305, 349
Csp6I GTAC 2 cut(s) 497, 521
CviAII CATG 2 cut(s) 313, 585
CviQI GTAC 2 cut(s) 497, 521
DdeI CTNAG 1 cut(s) 442
DinI GGCGCC 1 cut(s) 91
DrdI GACNNNNNNGTC 1 cut(s) 380
DseDI GACNNNNNNGTC 1 cut(s) 380
EaeI YGGCCR 2 cut(s) 151, 240
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 225
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 243, 505
Eco24I GRGCYC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 109
Eco52I CGGCCG 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 84
EcoNI CCTNNNNNAGG 2 cut(s) 84, 504
EcoRII CCWGG 2 cut(s) 105, 435
EcoT14I CCWWGG 2 cut(s) 243, 505
EcoT38I GRGCYC 1 cut(s) 443
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 2 cut(s) 243, 505
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 2 cut(s) 316, 588
FaiI YATR 6 cut(s) 314, 408, 450, 462, 519, 586
FatI CATG 2 cut(s) 312, 584
FblI GTMKAC 1 cut(s) 285
FriOI GRGCYC 1 cut(s) 443
FspBI CTAG 2 cut(s) 18, 453
GlaI GCGC 4 cut(s) 64, 91, 193, 300
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 153, 162, 242
HapII CCGG 5 cut(s) 72, 219, 335, 344, 367
HgaI GACGC 2 cut(s) 305, 349
HhaI GCGC 4 cut(s) 65, 92, 194, 301
Hin1I GRCGYC 3 cut(s) 90, 135, 341
Hin1II CATG 2 cut(s) 316, 588
Hin6I GCGC 4 cut(s) 63, 90, 192, 299
HinP1I GCGC 4 cut(s) 63, 90, 192, 299
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 2 cut(s) 156, 510
HpaII CCGG 5 cut(s) 72, 219, 335, 344, 367
HphI GGTGA 3 cut(s) 247, 349, 361
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 2 cut(s) 133, 515
Hpy188III TCNNGA 2 cut(s) 323, 444
Hpy8I GTNNAC 1 cut(s) 286
Hpy99I CGWCG 5 cut(s) 137, 140, 182, 290, 293
HpyAV CCTTC 2 cut(s) 130, 469
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 393, 489
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 189, 213, 501
HpyF3I CTNAG 1 cut(s) 442
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 3 cut(s) 90, 135, 341
Hsp92II CATG 2 cut(s) 316, 588
HspAI GCGC 4 cut(s) 63, 90, 192, 299
KasI GGCGCC 1 cut(s) 89
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
LmnI GCTCC 4 cut(s) 221, 247, 337, 369
Lsp1109I GCAGC 2 cut(s) 44, 288
MaeI CTAG 2 cut(s) 18, 453
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 4 cut(s) 235, 275, 337, 371
MboII GAAGA 3 cut(s) 16, 19, 572
MhlI GDGCHC 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 156
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 57, 150
MspI CCGG 5 cut(s) 72, 219, 335, 344, 367
MspR9I CCNGG 2 cut(s) 107, 437
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 2 cut(s) 107, 437
MvnI CGCG 4 cut(s) 63, 194, 207, 299
MwoI GCNNNNNNNGC 4 cut(s) 159, 189, 213, 501
NaeI GCCGGC 1 cut(s) 73
NarI GGCGCC 1 cut(s) 90
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 316, 588
NlaIV GGNNCC 4 cut(s) 91, 249, 333, 365
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 3 cut(s) 235, 337, 371
PaeR7I CTCGAG 1 cut(s) 84
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 141
PctI GAATGC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 156, 510
PluTI GGCGCC 1 cut(s) 93
PsiI TTATAA 1 cut(s) 462
Psp6I CCWGG 2 cut(s) 105, 435
PspGI CCWGG 2 cut(s) 105, 435
PspN4I GGNNCC 4 cut(s) 91, 249, 333, 365
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 84
RsaI GTAC 2 cut(s) 498, 522
RsaNI GTAC 2 cut(s) 497, 521
SalI GTCGAC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 107, 437
SduI GDGCHC 1 cut(s) 443
SetI ASST 5 cut(s) 138, 218, 237, 351, 502
SfcI CTRYAG 1 cut(s) 378
SfiI GGCCNNNNNGGCC 1 cut(s) 159
SfoI GGCGCC 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 2 cut(s) 18, 453
StyD4I CCNGG 2 cut(s) 105, 435
StyI CCWWGG 2 cut(s) 243, 505
TaiI ACGT 1 cut(s) 138
TaqI TCGA 4 cut(s) 85, 260, 285, 384
TatI WGTACW 1 cut(s) 520
TfiI GAWTC 2 cut(s) 156, 510
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseFI GTSAC 3 cut(s) 235, 337, 371
TseI GCWGC 2 cut(s) 57, 301
Tsp45I GTSAC 3 cut(s) 235, 337, 371
TspDTI ATGAA 1 cut(s) 423
TspGWI ACGGA 3 cut(s) 133, 306, 588
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 2 cut(s) 84, 504
XhoI CTCGAG 1 cut(s) 84
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 2 cut(s) 18, 453
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.